Organization
Department of Comprehensive Technical Solutions Technical Expert staff
Position
Technical Expert staff
External link
UEKI Hideo
Degree
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Master of Agricultural Science ( 2003.3 Okayama University )
Research Areas
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Life Science / Urology
Papers
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The Necroptotic Process-Related Signature Predicts Immune Infiltration and Drug Sensitivity in Kidney Renal Papillary Cell Carcinoma. International journal
Wenfeng Lin, Ruizhi Xue, Hideo Ueki, Peng Huang
Current cancer drug targets 2024.4
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Significance of UGT1A6, UGT1A9, and UGT2B7 genetic variants and their mRNA expression in the clinical outcome of renal cell carcinoma. International journal
Jun Matsumoto, Anzu Nishimoto, Shogo Watari, Hideo Ueki, Shoya Shiromizu, Naohiro Iwata, Tatsuaki Takeda, Soichiro Ushio, Makoto Kajizono, Masachika Fujiyoshi, Toshihiro Koyama, Motoo Araki, Koichiro Wada, Yoshito Zamami, Yasutomo Nasu, Noritaka Ariyoshi
Molecular and cellular biochemistry 478 ( 8 ) 1779 - 1790 2022.12
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Identification of MICALL2 as a Novel Prognostic Biomarker Correlating with Inflammation and T Cell Exhaustion of Kidney Renal Clear Cell Carcinoma
Wenfeng Lin, Wenwei Chen, Jisheng Zhong, Hideo Ueki, Abai Xu, Masami Watanabe, Motoo Araki, Chunxiao Liu, Yasutomo Nasu, Peng Huang
JOURNAL OF CANCER 13 ( 3 ) 1214 - 1228 2022
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Relevance of CYP3A5 Expression on the Clinical Outcome of Patients With Renal Cell Carcinoma. Reviewed International journal
Jun Matsumoto, Yumi Kotera, Shogo Watari, Koichi Takeuchi, Hideo Ueki, Toshihiro Koyama, Koichiro Wada, Masachika Fujiyoshi, Yasutomo Nasu, Noritaka Ariyoshi
Anticancer research 41 ( 5 ) 2511 - 2521 2021.5
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Repurposing of posaconazole as a hedgehog/SMO signaling inhibitor for embryonal rhabdomyosarcoma therapy. International journal
Jingkai Sun, Wenfeng Lin, Chaoming Li, Hideo Ueki, Ruizhi Xue, Takuya Sadahira, Hao Hu, Koichiro Wada, Na Li, Chunxiao Liu, Motoo Araki, Abai Xu, Peng Huang
American journal of cancer research 11 ( 9 ) 4528 - 4540 2021
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Tumor suppressor REIC/Dkk‑3 and its interacting protein SGTA inhibit glucocorticoid receptor to nuclear transport Reviewed
Takehiro Iwata, Takuya Sadahira, Kazuhiko Ochiai, Hideo Ueki, Takanori Sasaki, Peng Haung, Motoo Araki, Toyohiko Watanabe, Yasutomo Nasu, Masami Watanabe
Experimental and Therapeutic Medicine 2020.5
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Role of Egr1 on Pancreatic Endoderm Differentiation Reviewed
Tsugata Takako, Nikoh Naruo, Kin Tatsuya, Miyagi-Shiohira Chika, Nakashima Yoshiki, Saitoh Issei, Noguchi Yasufumi, Ueki Hideo, Watanabe Masami, Kobayashi Naoya, Shapiro Andrew, M. James, Noguchi Hirofumi
CELL MEDICINE 10 2018.5
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Induction of cells with prostate cancer stem-like properties from mouse induced pluripotent stem cells via conditioned medium. Reviewed International journal
Naijin Xu, Xiezhao Li, Masami Watanabe, Hideo Ueki, Hao Hu, Na Li, Motoo Araki, Koichiro Wada, Abai Xu, Chunxiao Liu, Yasutomo Nasu, Peng Huang
American journal of cancer research 8 ( 8 ) 1624 - 1632 2018
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Properties of the feline tumour suppressor reduced expression in immortalized cells (REIC/Dkk-3) Reviewed
K. Ochiai, H. Oda, S. Shono, Y. Kato, S. Sugihara, S. Nakazawa, D. Azakami, M. Michishita, E. Onozawa, M. Bonkobara, T. Sako, L. Shun-Ai, H. Ueki, M. Watanabe, T. Omi
VETERINARY AND COMPARATIVE ONCOLOGY 15 ( 4 ) 1181 - 1186 2017.12
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Robust cancer-specific gene expression by a novel cassette with hTERT and CMV promoter elements Reviewed
Masakiyo Sakaguchi, Takuya Sadahira, Hideo Ueki, Rie Kinoshita, Hitoshi Murata, Ken-Ichi Yamamoto, Junichiro Futami, Yasutomo Nasu, Kazuhiko Ochiai, Hiromi Kumon, Nam-Ho Huh, Masami Watanabe
ONCOLOGY REPORTS 38 ( 2 ) 1108 - 1114 2017.8
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The Downregulation of the Expression of CD147 by Tumor Suppressor REIC/Dkk-3, and Its Implication in Human Prostate Cancer Cell Growth Inhibition Reviewed
Akihiro Mori, Masami Watanabe, Takuya Sadahira, Yasuyuki Kobayashi, Yuichi Ariyoshi, Hideo Ueki, Koichiro Wada, Kazuhiko Ochiai, Shun-Ai Li, Yasutomo Nasu
ACTA MEDICA OKAYAMA 71 ( 2 ) 135 - 142 2017.4
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Tumor suppressor REIC/DKK-3 and co-chaperone SGTA: Their interaction and roles in the androgen sensitivity Reviewed
Kazuhiko Ochiai, Masami Morimatsu, Yuiko Kato, Toshina Ishiguro-Oonuma, Chihiro Udagawa, Oumaporn Rungsuriyawiboon, Daigo Azakami, Masaki Michishita, Yuichi Ariyoshi, Hideo Ueki, Yasutomo Nasu, Hiromi Kumon, Masami Watanabe, Toshinori Omi
ONCOTARGET 7 ( 3 ) 3273 - 3286 2016.1
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A vaccine strategy with multiple prostatic acid phosphatase-fused cytokines for prostate cancer treatment Reviewed
Kei Fujio, Masami Watanabe, Hideo Ueki, Shun-Ai Li, Rie Kinoshita, Kazuhiko Ochiai, Junichiro Futami, Toyohiko Watanabe, Yasutomo Nasu, Hiromi Kumon
ONCOLOGY REPORTS 33 ( 4 ) 1585 - 1592 2015.4
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Potential Factors for the Differentiation of ESCs/iPSCs Into Insulin-Producing Cells. Reviewed
Tsugata T, Nikoh N, Kin T, Saitoh I, Noguchi Y, Ueki H, Watanabe M, James Shapiro AM, Noguchi H
Cell medicine 7 ( 2 ) 83 - 93 2015.2
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Significant association between the Axin2 rs2240308 single nucleotide polymorphism and the incidence of prostate cancer Reviewed
Chao Ma, Chunxiao Liu, Peng Huang, Haruki Kaku, Jie Chen, Kai Guo, Hideo Ueki, Akiko Sakai, Yasutomo Nasu, Hiromi Kumon, Kenji Shimizu, Masami Watanabe
ONCOLOGY LETTERS 8 ( 2 ) 789 - 794 2014.8
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Dramatic Increase in Expression of a Transgene by Insertion of Promoters Downstream of the Cargo Gene Reviewed
Masakiyo Sakaguchi, Masami Watanabe, Rie Kinoshita, Haruki Kaku, Hideo Ueki, Junichiro Futami, Hitoshi Murata, Yusuke Inoue, Shun-Ai Li, Peng Huang, Endy Widya Putranto, I. Made Winarsa Ruma, Yasutomo Nasu, Hiromi Kumon, Nam-ho Huh
MOLECULAR BIOTECHNOLOGY 56 ( 7 ) 621 - 630 2014.7
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Immunological aspects of REIC/Dkk-3 gene therapy : the mechanism of the robust anti-tumor effects. Reviewed
Masami Watanabe, Peng Huang, Fernando Abarzua, Haruki Kaku, Katsumi Sasaki, Hideo Ueki, Toyohiko Wananabe, Yasutomo Nasu, Hiromi Kumon
JOURNAL OF GENE MEDICINE 16 ( 7-8 ) 226 - 227 2014.7
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A novel gene expression system strongly enhances the anticancer effects of a REIC/Dkk-3-encoding adenoviral vector Reviewed
Masami Watanabe, Masakiyo Sakaguchi, Rie Kinoshita, Haruki Kaku, Yuichi Ariyoshi, Hideo Ueki, Ryuta Tanimoto, Shin Ebara, Kazuhiko Ochiai, Junichiro Futami, Shun-Ai Li, Peng Huang, Yasutomo Nasu, Nam-Ho Huh, Hiromi Kumon
ONCOLOGY REPORTS 31 ( 3 ) 1089 - 1095 2014.3
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Cancer stem cell-like characteristics of a CD133+ subpopulation in the J82 human bladder cancer cell line. Reviewed International journal
Peng Huang, Masami Watanabe, Haruki Kaku, Hideo Ueki, Hirofumi Noguchi, Morito Sugimoto, Takeshi Hirata, Hiroshi Yamada, Kohji Takei, Shaobo Zheng, Kai Xu, Yasutomo Nasu, Yasuyuki Fujii, Chunxiao Liu, Hiromi Kumon
Molecular and clinical oncology 1 ( 1 ) 180 - 184 2013.1
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Preclinical biodistribution and safety study of reduced expression in immortalized cells/Dickkopf-3-encoding adenoviral vector for prostate cancer gene therapy Reviewed
Morito Sugimoto, Masami Watanabe, Haruki Kaku, Shun-Ai Li, Hirofumi Noguchi, Hideo Ueki, Masakiyo Sakaguchi, Nam-Ho Huh, Yasutomo Nasu, Hiromi Kumon
ONCOLOGY REPORTS 28 ( 5 ) 1645 - 1652 2012.11
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A novel gene expression system for detecting viable bladder cancer cells Reviewed
Hideo Ueki, Masami Watanabe, Haruki Kaku, Peng Huang, Shun-Ai Li, Kazuhiko Ochiai, Takeshi Hirata, Hirofumi Noguchi, Hiroshi Yamada, Kohji Takei, Yasutomo Nasu, Yuji Kashiwakura, Hiromi Kumon
INTERNATIONAL JOURNAL OF ONCOLOGY 41 ( 1 ) 135 - 140 2012.7
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Implications of transcriptional factor, OCT-4, in human bladder malignancy and tumor recurrence Reviewed
Peng Huang, Jie Chen, Lei Wang, Yanqun Na, Haruki Kaku, Hideo Ueki, Katsumi Sasaki, Ken Yamaguchi, Kai Zhang, Takashi Saika, Yasutomo Nasu, Masami Watanabe, Hiromi Kumon
MEDICAL ONCOLOGY 29 ( 2 ) 829 - 834 2012.6
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Advanced two-step transcriptional amplification as a novel method for cancer-specific gene expression and imaging Reviewed
Masami Watanabe, Hideo Ueki, Kazuhiko Ochiai, Peng Huang, Yasuyuki Kobayashi, Yasutomo Nasu, Katsumi Sasaki, Haruki Kaku, Yuji Kashiwakura, Hiromi Kumon
ONCOLOGY REPORTS 26 ( 4 ) 769 - 775 2011.10
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Tumor suppressor REIC/Dkk-3 interacts with the dynein light chain, Tctex-1 Reviewed
Kazuhiko Ochiai, Masami Watanabe, Hideo Ueki, Peng Huang, Yasuyuki Fujii, Yasutomo Nasu, Hirofumi Noguchi, Takeshi Hirata, Masakiyo Sakaguchi, Nam-ho Huh, Yuji Kashiwakura, Haruki Kaku, Hiromi Kumon
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 412 ( 2 ) 391 - 395 2011.8
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Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development Reviewed
Kai Zhang, Masami Watanabe, Yuji Kashiwakura, Shun-Al Li, Kohei Edamura, Peng Huang, Ken Yamaguchi, Yasutomo Nasu, Yasuyuki Kobayashi, Masakiyo Sakaguchi, Kazuhiko Ochiai, Hiroshi Yamada, Kohji Takei, Hideo Ueki, Nam-Ho Huh, Ming Li, Haruki Kaku, Yanqun Na, Hiromi Kumon
INTERNATIONAL JOURNAL OF ONCOLOGY 37 ( 6 ) 1495 - 1501 2010.12
MISC
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Advanced two-step transcriptional amplification as a novel method for cancer-specific gene expression and imaging
H. Kaku, H. Ueki, Y. Ariyoshi, S. Li, P. Huang, Y. Nasu, H. Kumon, M. Watanabe
HUMAN GENE THERAPY 24 ( 12 ) A154 - A154 2013.12
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山田聖, 植木英雄, 喜多洋一, 西沢けいと, 黒田昌治, 与座宏一, 北山雅彦, 石本政男
日本植物生理学会年会要旨集 46th ( 0 ) 227 - 491 2005.3
Presentations
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ダイズシストセンチュウ抵抗性遺伝子型を判別する分子マーカーの開発
植木 英雄
日本育種学会 第105回講演会 2004
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サツマイモにおけるレトロトランスポソンLTR配列の解析
植木 英雄
日本育種学会 第100回講演会 2001
Industrial property rights
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公文 裕巳, 渡部 昌実, 二見 淳一郎, 藤井 康之, 植木 英雄, 落合 和彦
Research Projects
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Oncolytic virus の多元的制癌性の解明と革新的癌治療への展開
Grant number：22K09526 2022.04 - 2025.03
日本学術振興会 科学研究費助成事業 基盤研究(C) 基盤研究(C)
植木 英雄
Grant amount：\3900000 （ Direct expense: \3000000 、 Indirect expense：\900000 ）
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骨髄由来免疫抑制細胞の抗癌免疫逃避機構の解明に基づく革新的癌創薬の探索
Grant number：21K09371 2021.04 - 2024.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)
黄 鵬
Grant amount：\4160000 （ Direct expense: \3200000 、 Indirect expense：\960000 ）
癌の研究開発において、癌免疫逃避機構に関する最近の注目すべき研究として、骨髄由来免疫抑制細胞（MDSC: myeloid-derived suppresser cell）が、癌に対する免疫監視機構や抗腫瘍免疫を負に制御して癌の悪性進展の中心的役割を担っていることが明らかにされつつある。本申請研究では、骨髄由来免疫抑制細胞の抗癌免疫逃避機構の解明に基づく革新的癌創薬の探索を目指している。本年度は、
①前立腺がん担癌マウスより末梢血を採収後FACS AriaでMDSSC細胞を分離し、MDSC細胞の表面マーカーの確認を行った。分離したMDSC細胞に対するREIC/Dkk-3タンバク質の抑制効果の観点から解析した。さらに、Ad-REICの抗癌作用に基づく免疫逃避応答に関する機序を解明し、その有効性を検証した。
②分離したMDSC細胞にREIC/Dkk-3タンパク質を作用させ、１分、30分、１時間、６時間、24時間後細胞内タンパク質を抽出し、または特異的siRNAによりノックダウンさせ、遺伝子-タンパク質発現変動をマイクロアレイやタンパク質抗体アレイ等により解析した。また、Western blot法により関連したパスウェイ内の各種タンパク質およびそのリン酸化の動態を実験した。
③細胞核内の標的遺伝子群（転写されるタンパク質群）の同定を行い、REIC/Dkk-3とMDSC細胞膜上での結合分子・受容体の同定解析を行った。 -
新規の癌抗原CD147を標的とする適応免疫最適化と尿路性器腫瘍での応用展開
2018.04 - 2022.03
日本学術振興会 科学研究費/基盤研究(B)
那須 保友
Grant type：Competitive
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制限増殖型アデノウィルスを用いた次世代遺伝子治療の基盤研究
2018.04 - 2021.03
日本学術振興会 科学研究費/基盤研究(C)
小林 泰之
Grant type：Competitive
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REIC/Dkk-3遺伝子による前立腺細胞増殖制御機構に関する研究
2018.04 - 2021.03
日本学術振興会 科学研究費/基盤研究(C)
高本 篤
Grant type：Competitive
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膀胱再生を目指した排尿平滑筋幹細胞の創出とその応用
2017.04 - 2021.03
日本学術振興会 科学研究費/基盤研究(C)
渡邉 豊彦
Grant type：Competitive
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前立腺癌幹細胞を標的とした新規遺伝子治療戦略の確立
2017.04 - 2020.03
日本学術振興会 科学研究費/基盤研究(C)
黄 鵬
Grant type：Competitive
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新規のチェックポイント阻害薬による腫瘍内免疫疲弊解除機構の解明
2017.04 - 2020.03
日本学術振興会 科学研究費/基盤研究(C)
定平 卓也
Grant type：Competitive
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Optimisation of a cancer detection and isolation technique using hTERT promoter, and establishment of its applicational foundation
Grant number：16K11004 2016.04 - 2019.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Ueki Hideo
Authorship：Principal investigator Grant type：Competitive
Grant amount：\4680000 （ Direct expense: \3600000 、 Indirect expense：\1080000 ）
A novel gene expression system that dramatically increases promoter activity while maintaining cancer specificity can be used as a new technology to strongly label and detect very few free cancer cells. The optimum conditions were also examined, and data for practical use of in vitro diagnostic methods were obtained. In addition, its usefulness has been confirmed as a technique for collecting detected free cancer cells and establishing them as cell lines.
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Regeneration strategy of the functional renal corpuscle using renal stem cells
Grant number：16K15689 2016.04 - 2019.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
Watanabe Masami, NASU YASUTOMO, UEKI HIDEO
Grant amount：\3250000 （ Direct expense: \2500000 、 Indirect expense：\750000 ）
In this study, we recognized the difficulty of the construction of three-dimensional structure and organ volume-regeneration. We performed basic studies based on our originally developed renal stem cells derived from human primary kidney cells by transfecting iPS four factors. In order to establish the focal injection, diffusion, transplantation technique of the stem cells, we used the In situ permeation system. We also performed basic studies in terms of the mesenchymal stem cells which is relevant to the renal stem cells derived from the glomerular endothelial cells.
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腎組織幹細胞における病的ストレス制御機構の解明
2015.04 - 2019.03
日本学術振興会 科学研究費/基盤研究(C)
荒木 元朗
Grant type：Competitive
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前立腺癌における悪性形質およびアンドロゲン不応性の一元的制御機構の解明
2015.04 - 2019.03
日本学術振興会 科学研究費/基盤研究(B)
渡部 昌実
Grant type：Competitive
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癌増悪因子：CD147 Emmprinを標的とした新規の抗癌免疫療法の基盤の確立
2015.04 - 2018.03
日本学術振興会 科学研究費/基盤研究(C)
小林 泰之
Grant type：Competitive
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前立腺癌抗原提示能を確立させる樹状細胞内分子機構の解明と創薬への展開
2015.04 - 2018.03
日本学術振興会 科学研究費/基盤研究(B)
那須 保友
Grant type：Competitive
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腫瘍融解性Ad-REICを用いた新規癌ワクチン療法の開発
2015.04 - 2017.03
日本学術振興会 科学研究費/若手研究(B)
黄 鵬
Grant type：Competitive
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尿路平滑筋幹細胞の創出と尿道括約筋再生のための基盤的研究
2014.04 - 2017.03
日本学術振興会 科学研究費/基盤研究(C)
渡邉 豊彦
Grant type：Competitive
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アンドロゲン分泌性幹細胞の創出とその応用基盤の確立
2013.04 - 2017.03
日本学術振興会 科学研究費/基盤研究(C)
杉本 盛人
Grant type：Competitive
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Development of a novel technique for the detection and isolation of disseminated cancer cells, and establishment of its application foundation
Grant number：25462478 2013.04 - 2016.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
UEKI HIDEO, NASU Yasutomo
Authorship：Principal investigator Grant type：Competitive
Grant amount：\4810000 （ Direct expense: \3700000 、 Indirect expense：\1110000 ）
The purpose of this study is to conduct foundational research for development of a novel technique for the detection and isolation of disseminated cancer cells with our original gene expression systems. The reporter gene, GFP expression under the system was investigated in various cancer and normal cells and it was confirmed that the system can increase the cancer cell-specific gene expression. It was also confirmed that the GFP encoding plasmid system allowed in vitro selective visualization of floating human cancer cells in mixed cell culture containing 10000-fold more normal blood cells.
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腎組織幹細胞の誘導・分離に基づく腎再生研究基盤の確立
2013.04 - 2016.03
日本学術振興会 科学研究費/挑戦的萌芽研究
渡部 昌実
Grant type：Competitive
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尿路癌に対する次世代癌免疫療法の創成
2013.04 - 2015.03
日本学術振興会 科学研究費/基盤研究(C)
賀来 春紀
Grant type：Competitive
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2012.12 - 2013.11
科学技術振興機構 研究成果最適展開支援プログラムＡ-ＳＴＥＰ
黄 鵬
Grant type：Competitive
本申請課題は、PETイメージングによる遺伝子治療の可視化に基づく遺伝子治療の有効性、安全性の評価法の確立を目指したものであり、その目標を概ね達成できたと考える。腫瘍マーカーとイメージング装置IVISでin vivoでトレース可能なRM9細胞を用いて免疫正常マウスで皮下担がんモデルを作製し、その腫瘍内および静脈内にAd-HSV1-tk剤を投与、3日後、5日後、7日後に、PET・IVIS撮像を実施した。特に、PETイメージングによるがん遺伝子治療評価モデルの構築のために、遺伝子治療で用いるHSV1-tk遺伝子に特異的なPETレポータープローブ: [18F]FMAUを合成し、その遺伝子発現の可視化に成功した。今後、これらの遺伝子発現の可視化条件を最適化する研究を継続して行う。
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ＲＥＩＣ／Ｄｋｋ―３遺伝子治療による自己癌ワクチン化療法の基盤解析
2011.04 - 2014.03
日本学術振興会 科学研究費/基盤研究(B)
公文 裕巳
Grant type：Competitive
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Basic research of smooth muscle cells generated from induced pluripotent stem cells for urethral tissue engineering.
Grant number：23592369 2011.04 - 2014.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
WATANABE Toyohiko, WATANABE Masami, UEKI Hideo
Grant type：Competitive
Stress urinary incontinence is associated with a reduced quality of life. An intact urethral sphincter is mandatory to maintain urinary continence. Adult stem cell injection therapy for the regenerative repair of an impaired sphincter is currently at the forefront of research. Recently, the establishment of iPS cells has offered a novel therapeutic strategy to generate patient-specific stem cell lines. Here we have investigated whether iPS cells are able to differentiate into smooth muscle cells (SMCs) in vitro. Human iPS cell monolayers were treated with 10-5 mol/L all-trans retinoid acid (RA). After 7 days of RA treatment, we found that iPS cells highly expressed the SMC-markers including SMa-actin, SM myosin HC, myocardin, and desmin determined by immunofluorescence. Our date have established a simple iPS-SMC system to generate SMCs in vitro, which has tremendous potential to generate individualized SMCs for urethral tissue engineering and continence recover.
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がん治療遺伝子REICによるナノバイオ標的医療の創成
2010 - 2012
岡山県 特別電源所在県科学技術振興事業 研究委託事業
公文 裕巳
Grant type：Competitive
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局所がん病巣を標的とする革新的生物製剤治療システムの開発
2010 - 2011
経済産業省 地域イノベーション創出研究開発事業
公文 裕巳
Grant type：Competitive
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播種性癌細胞検出・分離のための新技術の開発
2010
両備てい園記念財団 研究助成金
渡部 昌実
Grant type：Competitive