Faculty Profiles - HORIGUCHI Shigeru

Papers - HORIGUCHI Shigeru

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アンメットメディカルニーズへの挑戦当院における膵消化管神経内分泌腫瘍に対するペプチド受容体放射性核種療法(PRRT)導入後の現状

藤井佑樹, 堀口繁, 内田大輔, 松本和幸, 加藤博也

日本消化器病学会中国支部例会・日本消化器内視鏡学会中国支部例会プログラム・抄録集 118回・129回 77 - 77 2022.11

Optimization of Isolation Method for Extracellular Vesicles from Pancreatic Juice and Impact of Protease Activity. International journal

Koichiro Tsutsumi, Eijiro Ueta, Hironari Kato, Kazuyuki Matsumoto, Shigeru Horiguchi, Hiroyuki Okada

Digestive diseases and sciences 67 ( 10 ) 4797 - 4804 2022.10

Language：English Publishing type：Research paper (scientific journal)

BACKGROUNDS: Pancreatic juice (PJ) is directly associated with pancreatic lesions, including pancreatic ductal cancer and intraductal papillary mucinous neoplasm-derived cancer. Therefore, EVs secreted from these lesions into PJ can be promising biomarkers for early diagnosis. However, there are limited data from analysis of EVs in PJ samples. AIMS AND METHODS: We aimed to determine the stability of EVs in PJ collected using endoscopic naso-pancreatic drainage (ENPD) tubes as well as catheter during endoscopic retrograde cholangiography (ERCP), with or without the impact of positive protease activity, and optimize the EV isolation method. RESULTS: Size exclusion chromatography was found to be an optimal isolation method for EVs in PJ as it achieved higher recovery and purity of EVs compared with differential ultracentrifugation and polymer-based precipitation. Approximately 40% of the PJ samples collected during ERCP and more than 90% of those collected using ENPD tubes had positive protease activity. In vitro exposure to room temperature for less than 3 h was harmless to the structure of double-membrane EVs in PJ and the expression levels of TSG101, even with positive protease activity. CONCLUSIONS: We clarified the physiobiological status of EVs in PJ and optimized the EV isolation method using suitable PJ samples; these findings can be utilized to discover biomarkers for cancer diagnosis and elucidate their function.

DOI： 10.1007/s10620-021-07339-x

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人工知能を用いた膵癌におけるEUS-FNAの迅速細胞診診断

藤井佑樹, 山崎辰洋, 松本和幸, 堀口繁, 堤康一郎, 井上博文, 谷本太郁由, 加藤博也, 河原祥朗

日本消化器病学会雑誌 119 ( 臨増大会 ) A783 - A783 2022.10

胆道癌の新たな治療戦略(遺伝子パネル・分子標的・術前治療) 当院における肝内胆管癌のがんゲノム医療の現状について

堀口繁, 松本和幸, 加藤博也

日本消化器病学会雑誌 119 ( 臨増大会 ) A586 - A586 2022.10

A case of endoscopic retrograde cholangiopancreatography-related main pancreatic duct perforation salvaged by endoscopic ultrasonography-guided pancreatic duct drainage.

Ryosuke Sato, Kazuyuki Matsumoto, Akihiro Matsumi, Kosaku Morimoto, Hiroyuki Terasawa, Yuki Fujii, Tatsuhiro Yamazaki, Koichiro Tsutsumi, Shigeru Horiguchi, Hironari Kato

Clinical journal of gastroenterology 15 ( 6 ) 1179 - 1184 2022.9

Language：English Publishing type：Research paper (scientific journal)

We herein report a 78-year-old man who underwent endoscopic retrograde cholangiopancreatography (ERCP) to examine main pancreatic duct (MPD) stenosis. During ERCP, MPD perforation occurred due to the cytology brush maneuver. Endoscopic pancreatic stenting to bridge the perforated site failed because the MPD was bent and formed a loop. Thus, we placed the stent at the proximal perforated side. The patient developed retroperitoneal perforation and pancreatic fistula with infection, showing a worsening condition. Pancreatic duct drainage was not effective, so we performed endoscopic ultrasonography-guided pancreatic duct drainage. Subsequently, he gradually improved and was discharged 3 months after initial ERCP.

DOI： 10.1007/s12328-022-01699-1

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Stent-in-stent techniqueにて抜去した胆管金属ステントの2例

松三明宏, 松本和幸, 小幡泰介, 織田崇志, 菊池達也, 宮本和也, 森本光作, 寺澤裕之, 藤井佑樹, 山崎辰洋, 内田大輔, 堀口繁, 堤康一郎, 加藤博也

胆道 36 ( 3 ) 349 - 349 2022.9

生体肝移植後胆管狭窄に対する内視鏡治療

山崎辰洋, 松本和幸, 加藤博也, 松三明宏, 宮本和也, 寺澤裕之, 藤井佑樹, 内田大輔, 堀口繁, 堤康一郎

胆道 36 ( 3 ) 344 - 344 2022.9

Endoscopic ultrasonography-guided bilateral drainage with antegrade stenting in patient with malignant hilar biliary obstruction after bowel reconstruction. International journal

Ryosuke Sato, Kazuyuki Matsumoto, Hiroyuki Terasawa, Yuki Fujii, Tatsuhiro Yamazaki, Shigeru Horiguchi, Hironari Kato

Endoscopy 54 ( S 02 ) E1026-E1027 2022.8

Double guidewire technique stabilization procedure for endoscopic ultrasound-guided hepaticogastrostomy involving modifying the guidewire angle at the insertion site. International journal

Yuki Fujii, Hironari Kato, Hitomi Himei, Eijiro Ueta, Taiji Ogawa, Hiroyuki Terasawa, Tatsuhiro Yamazaki, Kazuyuki Matsumoto, Shigeru Horiguchi, Koichiro Tsutsumi, Hiroyuki Okada

Surgical endoscopy 36 ( 12 ) 8981 - 8991 2022.8

Language：English Publishing type：Research paper (scientific journal)

BACKGROUND AND AIMS: Endoscopic ultrasonography-guided hepaticogastrostomy (EUS-HGS) is often performed using a single guidewire (SGW), but the efficacy of the double guidewire (DGW) technique during endoscopic ultrasonography-guided biliary drainage has been reported. We evaluated the efficacy of the DGW technique for EUS-HGS, focusing on the guidewire angle at the insertion site. METHODS: This retrospective cohort study included consecutive patients who underwent EUS-HGS between April 2012 and March 2021. We measured the guidewire angle at the insertion site using still fluoroscopic imaging. We compared the clinical outcomes of EUS-HGS with the DGW and SGW techniques. The factors associated with successful cannula insertion, need for additional fistula dilation and adverse event rate were assessed by a logistic regression multivariable analysis. RESULTS: The DGW group showed higher technical (p = 0.020) and clinical success rates (p = 0.016) than the SGW group, which showed more adverse events (p = 0.017) than the DGW group. Successful cannula insertion was associated with a guidewire angle > 137° and an uneven double-lumen cannula. The DGW technique made the guidewire angle obtuse at the insertion site (p < 0.0001). A guidewire angle ≤ 137° (OR, 35.6; 95% CI, 1.70-744; p = 0.0045) and intrahepatic bile duct diameter of the puncture site ≤ 3.0 mm (OR, 14.4; 95% CI, 1.37-152; p = 0.0056) were risk factors for needing additional fistula dilation in a multivariate analysis, and additional dilation was a significant predictive factor for adverse events (OR, 8.3; 95% CI, 0.9-77; p = 0.026). CONCLUSIONS: The DGW technique can modify the guidewire angle at the insertion site and facilitate stent deployment with few adverse events.

DOI： 10.1007/s00464-022-09350-3

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『胆膵疾患と性差』分枝型IPMNの性差による画像的特徴と長期経過の検討

宮本和也, 松本和幸, 松三明宏, 森本光作, 寺澤裕之, 藤井佑樹, 山崎辰洋, 内田大輔, 堀口繁, 堤康一郎, 加藤博也

日本高齢消化器病学会誌 25 ( 1 ) 161 - 161 2022.7

Successful removal of an uncovered metallic stent using peroral direct cholangioscopy and the stent-in-stent technique. International journal

Akihiro Matsumi, Kazuyuki Matsumoto, Tatsuhiro Yamazaki, Shigeru Horiguchi, Koichiro Tsutsumi, Hironari Kato, Hiroyuki Okada

Endoscopy 2022.6

急性膵炎を契機として発見された膵頭部mixed type SCNの1例

佐藤亮介, 松三明宏, 上田英次郎, 寺澤裕之, 姫井人美, 森本光作, 小川泰司, 藤井佑樹, 山崎辰洋, 松本和幸, 堀口繁, 堤康一郎, 加藤博也, 岡田裕之

日本消化器病学会中国支部例会プログラム・抄録集 117回 89 - 89 2022.6

膵管内乳頭粘液性腫瘍由来癌との術前鑑別が困難であった限局型自己免疫性膵炎の一例

菊池達也, 藤井佑樹, 松本和幸, 松三明宏, 森本孝作, 寺澤裕之, 山崎辰洋, 堀口繁, 堤康一郎, 加藤博也

日本消化器病学会中国支部例会プログラム・抄録集 117回 92 - 92 2022.6

多発肝腫瘤を伴う胆嚢腫瘤に対してEndosheatherを用いERCP下胆嚢生検を行った1例

森本光作, 山崎辰洋, 小幡泰介, 織田崇志, 上田英次郎, 姫井人美, 松三明宏, 小川泰司, 寺澤裕之, 藤井佑樹, 松本和幸, 堀口繁, 堤康一郎, 加藤博也, 岡田裕之

日本消化器内視鏡学会中国支部例会 128回 116 - 116 2022.5

膵癌術後肝転移再発による悪性肝門部胆管閉塞に対して超音波内視鏡下両葉肝内胆管ドレナージを行った1例

佐藤亮介, 藤井佑樹, 松本和幸, 松三明宏, 森本光作, 寺澤裕之, 山崎辰洋, 堀口繁, 加藤博也

日本消化器内視鏡学会中国支部例会 128回 119 - 119 2022.5

Laminin 511-E8 Fragment Offers Superior Adhesion Properties for Gastric Cancer Cells Compared with Full-Length Laminin 511. Reviewed International journal

Masaya Iwamuro, Hidenori Shiraha, Mayu Kobashi, Shigeru Horiguchi, Hiroyuki Okada

Current issues in molecular biology 44 ( 4 ) 1539 - 1551 2022.4

Language：English Publishing type：Research paper (scientific journal)

BACKGROUND: The interaction between cancer cells and laminin (Ln) is a key event in tumor invasion and metastasis. Previously, we determined the effect of full-length Ln511 on gastric cancer cells. However, the interactions between the Ln511-E8 fragment, a truncated protein of Ln511, and gastric cancer cells have not been investigated. METHODS: We investigated the adhesion properties of gastric cancer cells to full-length Ln511 and Ln511-E8 fragments. RESULTS: The proliferation of four gastric cancer cell lines (SH-10-TC, MKN74, SC-6-JCK, and MKN45) was highest on the Ln511-E8 fragment. Further, a larger cytoplasm was observed in SH-10-TC and MKN74 cells cultured on full-length Ln511 or Ln511-E8 fragments. The percentage of adhesive cells was highest on the Ln511-E8 fragment in all four cell lines. Moreover, adhesion of the gastric cancer cells to Ln511-E8 fragment-coated plates was reduced by the Cdc42 GTPase inhibitor in a dose-dependent manner, suggesting the involvement of Cdc42 in the Ln511-E8 fragment-induced enhanced adhesion of gastric cancer cells. CONCLUSIONS: The Ln511-E8 fragment had a greater impact on the adhesion, morphology, and proliferation of gastric cancer cells than full-length laminin. Thus, the Ln511-E8 fragment is suitable for investigating the interaction between gastric cancer cells and extracellular matrices in tumor invasion and metastasis.

DOI： 10.3390/cimb44040105

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Endoscopic removal of proximally migrated stents using a double-balloon enteroscope in patients with bowel reconstruction (with video). International journal

Takashi Oda, Kazuyuki Matsumoto, Eijiro Ueta, Hitomi Himei, Taiji Ogawa, Hiroyuki Terasawa, Yuki Fujii, Tatsuhiro Yamazaki, Shigeru Horiguchi, Koichiro Tsutsumi, Hironari Kato, Hiroyuki Okada

DEN open 2 ( 1 ) e32 2022.4

Language：English

Endoscopic migrated stent removal using a balloon-assisted enteroscope is technically difficult in patients with bowel reconstruction. We report the treatment outcomes and endoscopic removal methods for migrated stents using a double-balloon enteroscope (DBE). We retrospectively studied 12 patients with stent migration into the main pancreatic duct (MPD) or bile duct who underwent bowel reconstruction between January 2012 and June 2020. The successful removal rates in the MPD (n = 3) and the bile duct (n = 9) were 66.7% (2/3) and 88.9% (8/9), respectively. The removal techniques included the indirect method (n = 3), the direct method (n = 4), and a combination of indirect and direct methods (n = 3). The removal devices included an extraction balloon catheter (n = 7), basket catheter (n = 5), biopsy forceps (n = 3), and snare (n = 2). Stent removal using a DBE was feasible and useful as the first treatment for patients with bowel reconstruction. The choice of the direct and/or indirect method according to the situation of the migrated stent is important.

DOI： 10.1002/deo2.32

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術後腸管症例におけるステント迷入に対するダブルバルン内視鏡を用いたステント抜去法

織田崇志, 松本和幸, 加藤博也, 小幡泰介, 上田英次郎, 姫井人美, 松三明宏, 森本光作, 小川泰司, 寺澤裕之, 藤井佑樹, 山崎辰洋, 堀口繁, 堤康一郎

Gastroenterological Endoscopy 64 ( Suppl.1 ) 765 - 765 2022.4

胃切後Roux-en-Y再建腸管を有する患者の総胆管結石治療におけるバルーン内視鏡下ERCPの治療戦略

小幡泰介, 堤康一郎, 加藤博也, 織田崇志, 森本光作, 姫井人美, 松三明宏, 上田英次郎, 小川泰司, 寺澤裕之, 藤井佑樹, 山崎辰洋, 松本和幸, 堀口繁, 岡田裕之

Gastroenterological Endoscopy 64 ( Suppl.1 ) 820 - 820 2022.4

胆管空腸吻合術後の肝内結石に対する直接胆道鏡の有用性の検討

松三明宏, 松本和幸, 加藤博也, 小幡泰介, 織田崇志, 上田英次郎, 姫井人美, 森本光作, 小川泰司, 寺澤裕之, 藤井佑樹, 山崎辰洋, 堀口繁, 堤康一郎, 岡田裕之

Gastroenterological Endoscopy 64 ( Suppl.1 ) 821 - 821 2022.4

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Papers - HORIGUCHI Shigeru