Updated on 2024/10/18

写真a

 
髙原　茉莉
 
Organization
Faculty of Interdisciplinary Science and Engineering in Health Systems Assistant Professor
Position
Assistant Professor
External link

Degree

  • 博士(工学） ( 九州大学 )

Research Interests

  • Aptamer

  • Drug delivery system

  • Bioengineering

  • Peptide

Research Areas

  • Nanotechnology/Materials / Bio chemistry

  • Nanotechnology/Materials / Polymer chemistry

  • Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering

Education

  • 九州大学大学院   工学府   化学システム工学専攻

    2012.4 - 2017.3

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  • Kyushu University   工学部   物質科学工学科

    2008.4 - 2012.3

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Research History

  • Kitakyushu National College of Technology   Department of Materials Science & Chemical Engineering   Assistant Professor

    2017.4

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  • Kyushu University   Department of Applied Chemistry, Faculty of Engineering   Research Fellowship for Young Scientists (DC1)

    2014.4 - 2017.3

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  • Texas Christian University   Department of Chemistry   Visiting Scholar

    2012.8 - 2012.12

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Professional Memberships

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Committee Memberships

  • The Materials Research Society of Japan (MRS-J)   Symposium organizer of annual meeting of MRS-J  

    2019.5   

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    Committee type：Academic society

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  • International Union of Materials Research Societies (IUMRS)   Committee of International Conference of Young Researchers on Advanced Materials  

    2019.3   

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    Committee type：Academic society

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Papers

  • Extending the Half-Life of a Protein in Vivo by Enzymatic Labeling with Amphiphilic Lipopeptides Reviewed International journal

    Mari Takahara, Shinichi Mochizuki, Rie Wakabayashi, Kosuke Minamihata, Masahiro Goto, Kazuo Sakurai, Noriho Kamiya

    Bioconjugate Chemistry   Accepted ( 4 )   655 - 660   2021.3

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

    Synthesis of lipid-protein conjugates is one of the significant techniques in drug delivery systems of proteins; however, the intact conjugation of a lipid and protein is yet challenging due to the hydrophobicity of lipid molecules. In order to facilitate easy handling of the lipid moiety in conjugation, we have focused on a microbial transglutaminase (MTG) that can ligate specific lysine (K) and glutamine (Q) residues in lipopeptides and a protein of interest. As MTG substrates, monolipid- and dilipid-fused amphiphilic short lipopeptide substrates (lipid-G3S-RHK or lipid2-KG3S-RHK) were designed. These amphiphilic lipopeptides and a model protein (enhanced green fluorescent protein, EGFP) fused with LLQG (LQ-EGFP) were both water-soluble, and thus lipid-protein conjugates were efficiently obtained through the MTG reaction with a >80% conversion rate of LQ-EGFP even using cholesterol-G3S-RHK. In vitro cell adhesion and in vivo half-life stability of the successfully obtained lipid-protein conjugates were evaluated, showing that the monocholesterol-G3S-RHK modification of a protein gave the highest cell adhesion efficiency and longest half-life time by formation of a stable albumin/lipid-protein complex.

    DOI： 10.1021/acs.bioconjchem.1c00027

    PubMed

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  • Preparation of an (inorganic/organic) hybrid hydrogel from a peptide oligomer and a tubular aluminosilicate nanofiber Reviewed

    Masaru Mukai, Mari Takahara, Akihiko Takada, Astushi Takahara

    RSC Advances   11 ( 9 )   4901 - 4905   2021.1

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Royal Society of Chemistry (RSC)  

    <p>‘Imogolite’, a tubular inorganic nanotube surface, was modified with a peptide oligomer to prepare a hybrid hydrogel.</p>

    DOI： 10.1039/d0ra09514a

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  • Synthetic Strategies for Artificial Lipidation of Functional Proteins Invited Reviewed

    Mari Takahara, Noriho Kamiya

    Chemistry-A European Journal   26 ( 21 )   4645 - 4655   2020.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/chem.201904568

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  • Enzymatic cell‐surface decoration with proteins using amphiphilic lipid‐fused peptide substrates Reviewed

    Mari Takahara, Rie Wakabayashi, Naoki Fujimoto, Kosuke Minamihata, Masahiro Goto, Noriho Kamiya

    Chemistry A European Journal(Inside Cover)   25 ( 30 )   7315 - 7321   2019.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/chem.201901274

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  • Design of Lipid-Protein Conjugates Using Amphiphilic Peptide Substrates of Microbial Transglutaminase Reviewed

    Mari Takahara, Rie Wakabayashi, Kosuke Minamihata, Masahiro Goto, Noriho Kamiya

    ACS Applied Bio Materials   1 ( 6 )   1823 - 1829   2018.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    Copyright © 2018 American Chemical Society. Lipid modification of proteins plays a significant role in regulating the cellular environment. Mimicking natural lipidated proteins is a key technique for assessing the function of proteins modified with lipids and also to render self-assembly of lipids to a target protein. Herein, we report a facile method of conjugating proteins with lipid-fused peptides under homogeneous physiological conditions by using the microbial transglutaminase (MTG) reaction. MTG catalyzes the cross-linking reaction between a specific glutamine (Q) in a protein and a lysine (K) in newly designed lipid-fused peptides. The water-soluble peptide substrates for lipid modification, C14-X-MRHKGS, were newly synthesized, where C14, X, and MRHKGS represent myristic acid, linker peptides composed of G, P, or S, and MTG-reactive K surrounded with basic amino acids, respectively. The MTG-mediated cross-linking reaction between a protein fused with LLQG at the C-terminus and C14-X-MRHKGS (5 molar eq) dissolved in a phosphate saline solution resulted in lipid-protein conjugates with yields of 70 to 100%. The anchoring ability of the obtained lipid-protein conjugates to cell membranes was dependent on the number of G residues in the GnS linker, suggesting that self-assembly and hydrophobicity of the GnS motif serves to enhance membrane anchoring of lipid-protein conjugates.

    DOI： 10.1021/acsabm.8b00271

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  • Design of lipid-protein conjugate with a self-assembling ability on a cell membrane by using microbial transglutaminase reaction Reviewed

    Takahara Mari, Wakabayashi Rie, Minamihata Kosuke, Goto Masahiro, Kamiya Noriho

    ACS Appl. Bio Mater., accepted. DOI: 10.1021/acsabm.8b00271.   2018.8

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  • Primary Amine-Clustered DNA Aptamer for DNA-Protein Conjugation Catalyzed by Microbial Transglutaminase Reviewed

    Mari Takahara, Rie Wakabayashi, Kosuke Minamihata, Masahiro Goto, Noriho Kamiya

    BIOCONJUGATE CHEMISTRY   28 ( 12 )   2954 - 2961   2017.12

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：AMER CHEMICAL SOC  

    DNA-protein conjugates are promising biomolecules for use in areas ranging from therapeutics to analysis because of the dual functionalities of DNA and protein. Conjugation requires site-specific and efficient covalent bond formation without impairing the activity of both biomolecules. Herein, we have focused on the use of a microbial transglutaminase (MTG) that catalyzes the cross-linking reaction between a glutamine residue and a primary amine. In a model bioconjugation, a highly MTG-reactive Gln (Q)-donor peptide (FYPLQMRG, FQ) was fused to enhanced green fluorescent protein (FQEGFP) and a primary amine clustered DNA aptamer was enzymatically synthesized as a novel aryl-acceptor substrate of MTG, whose combination leads to efficient and convenient preparation of DNA-protein conjugates with high purity. Dual functionality of the obtained DNA-EGFP conjugate was evaluated by discrimination of cancer cells via c-Met receptor recognition ability of the DNA aptamer. The DNA aptamer-EGFP conjugate only showed fluorescence toward cells with c-Met overexpression, indicating the retention of the biochemical properties of the DNA and EGFP in the conjugated form.

    DOI： 10.1021/acs.bioconjchem.7b00594

    Web of Science

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  • Salt-Switchable Artificial Cellulase Regulated by a DNA Aptamer Reviewed

    Mari Takahara, Geisa Aparecida Lopes Goncalves Budinova, Hikaru Nakazawa, Yutaro Mori, Mitsuo Umetsu, Noriho Kamiya

    BIOMACROMOLECULES   17 ( 10 )   3356 - 3362   2016.10

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：AMER CHEMICAL SOC  

    A novel artificial cellulase was developed by conjugating a DNA aptamer to an endoglucanase catalytic domain, thereby substituting the natural carbohydrate-binding module. Circular dichroism spectroscopy and adsorption isotherm showed the binding motif of cellulose-binding DNA aptamer (CelApt) was G-quadruplex and stem-loop structures stabilized in the presence of salts, and CelApt binding preferred the amorphous region of the solid cellulose. By introducing the revealed salt-switchable cellulose binding nature of CelApt into a catalytic domain of a cellulase, we created CelApt-catalytic domain conjugate possessing both controllable adsorption on the solid substrates and equal enzymatic activity to the wild-type cellulase. Thus potential use of a responsive DNA aptamer for biocatalysis at a solid surface was demonstrated.

    DOI： 10.1021/acs.biomac.6b01141

    Web of Science

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  • Enzymatic conjugation of multiple proteins on a DNA aptamer in a tail-specific manner Reviewed

    Mari Takahara, Kounosuke Hayashi, Masahiro Goto, Noriho Kamiya

    BIOTECHNOLOGY JOURNAL   11 ( 6 )   814 - 823   2016.6

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY-V C H VERLAG GMBH  

    Conjugation of single-strand DNA aptamers and enzymes has been of great significance in bio-analytical and biomedical applications because of the unlimited functions provided by DNA aptamer direction. Therefore, we developed efficient tailing of a DNA aptamer, with end-specific conjugation of multiple enzymes, through enzymatic catalysis. Terminal deoxynucleotidyl transferase (TdT) added multiple Z-Gln-Gly (Z-QG) moieties to the 3'-end of a DNA aptamer via the addition of Z-QG-modified deoxyuridine triphosphate (Z-QG-dUTP) and deoxynucleoside triphosphates (dNTPs). The resultant (Z-QG)(m)-(dN)(l)-aptamer, whose Z-QGs with dN spacers served as stickers for microbial transglutaminase (MTG), were crosslinked between the Z-QGs on the DNA and a substrate peptide sequence containing lysine (K), fused to a recombinant enzyme (i.e. bacterial alkaline phosphatase; BAP) by MTG. The incorporation efficiency of Z-QG moieties on the aptamer tail and the subsequent conjugation efficiency with multiple enzyme molecules were dramatically altered by the presence of dNTPs, revealing that a combination of Z-QG-dUTP/dTTP comprised the best labeling efficiency and corresponding properties during analytical performance. Thus, a novel optimized platform for designing (BAP)(n)-(dT)(l)-DNA aptamers is demonstrated for the first time in this article, offering unique opportunities for tailoring new types of covalent protein-nucleic acid conjugates in a controllable way.

    DOI： 10.1002/biot.201500560

    Web of Science

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  • Tailing DNA aptamers with a functional protein by two-step enzymatic reaction Reviewed

    Mari Takahara, Kounosuke Hayashi, Masahiro Goto, Noriho Kamiya

    Journal of Bioscience and Bioengineering   116 ( 6 )   660 - 665   2013.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    An efficient, quantitative synthetic strategy for aptamer-enzyme conjugates was developed by using a two-step enzymatic reaction. Terminal deoxynucleotidyl transferase (TdT) was used to first incorporate a Z-Gln-Gly (QG) modified nucleotide which can act as a glutamine donor for a subsequent enzymatic reaction, to the 3'-OH of a DNA aptamer. Microbial transglutaminase (MTG) then catalyzed the cross-linking between the Z-QG modified aptamers and an enzyme tagged with an MTG-reactive lysine containing peptide. The use of a Z-QG modified dideoxynucleotide (Z-QG-ddUTP) or a deoxyuridine triphosphate (Z-QG-dUTP) in the TdT reaction enables the controlled introduction of a single or multiple MTG reactive residues. This leads to the preparation of enzyme-aptamer and (enzyme)n-aptamer conjugates with different detection limits of thrombin, a model analyte, in a sandwich enzyme-linked aptamer assay (ELAA). Since the combination of two enzymatic reactions yields high site-specificity and requires only short peptide substrates, the methodology should be useful for the labeling of DNA/RNA aptamers with proteins. © 2013 The Society for Biotechnology, Japan.

    DOI： 10.1016/j.jbiosc.2013.05.025

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Books

  • How to Prepare Artificial Lipid-protein Conjugates

    （ Role： Joint author）

    2020.7 

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  • 細胞・生体分子の固定化と機能発現

    神谷典穂, 高原 茉莉（ Role： Contributor ,  第5章 セルロース結合性アプタマーを用いた人工セルラーゼの設計）

    シーエムシー出版  2018.4 

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MISC

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Presentations

  • Enzymatic synthesis of lipid-protein conjugates using amphiphilic lipopeptide

    Mari Takahara, Rie Wakabayashi, Masahiro Goto, Noriho Kamiya

    30th Annual Meeting of MRS-J  2020.12.10 

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    Event date： 2020.12.9 - 2020.12.11

    Language：Japanese   Presentation type：Poster presentation  

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  • Artificial lipidation process of a protein using post-translational enzyme Invited

    Mari Takahara, Rie Wakabayashi, Masahiro Goto, Noriho Kamiya

    MRM（Materials Research Meeting）Forum 2020  2020.12.8 

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    Event date： 2020.12.7 - 2020.12.9

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

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  • Current academic carrier after doctor course Invited

    Mari Takahara

    10th CSJ Chemistry Festa  2020.10.22 

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    Event date： 2020.10.20 - 2020.10.22

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

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  • Synthesis of CpG-(dA)m adjuvant using enzyme and polysaccharide Invited

    Mari Takahara, Takuya Matsunaga, Shinichi Mochizuki, Kazuo Sakurai

    SCEJ 51st Autumn Meeting (Alternative of SCEJ 85th Annual Meeting)  2020.9.26 

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    Event date： 2020.9.24 - 2020.9.26

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

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  • Synthesis of Hydrophilic lipid-protein conjugates in an enzymatic manner.

    Mari Takahara, Rie Wakabayashi, Masahiro Goto, Noriho Kamiya

    The 14th Bio-related chemistry symposium  2020.9.7 

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    Event date： 2020.9.7 - 2020.9.8

    Presentation type：Oral presentation (general)  

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  • Enzymatic labeling of a protein with lipopeptides for albumin interaction

    Mari Takahara, Rie Wakabayashi, Masahiro Goto, Noriho Kamiya

    ACS Fall 2020 National Meeting  2020.8.19 

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    Event date： 2020.8.17 - 2020.8.20

    Language：English   Presentation type：Oral presentation (general)  

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  • The controlled synthesis of polysaccharide/CpG-DNA complex in an enzymatic manner

    Mari Takahara, Shinichi Mochizuki, Kazuo Sakurai

    The 69th SPSJ Annual Meeting  2020.5.27 

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    Language：Japanese   Presentation type：Poster presentation  

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  • Enzymatic dual-lipidation of a protein in a site-specific manner

    Mari Takahara, Rie Wakabayashi, Masahiro Goto, Noriho Kamiya

    The 100th CSJ Annual Meeting  2020.3.25 

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    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Cell-surface decoration with lipid-protein conjugates synthesized in an enzymatic manner

    Cell-surface decoration with, lipid-protein conjugates synthesized in an enzymatic manner

    2019.11.27 

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    Language：English   Presentation type：Oral presentation (general)  

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  • 酵素反応を利用したDNA-(dA)m型アジュバントの合成

    高原 茉莉, 望月 慎一, 櫻井 和朗

    酵素工学研究会 第82回講演会  2019.11.1 

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  • In situ cell-surface decoration with a protein using amphiphilic enzyme substrates International conference

    Mari Takahara, Rie Wakabayashi, Kosuke Minamihata, Masahiro Goto, Noriho Kamiya

    The 18th Asian Pacific Confederation of Chemical Engineering Congress  2019.9.25 

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  • 細胞表面へのin situタンパク質ラベルを志向した両親媒性ペプチドの開発

    高原 茉莉, 若林 里衣, 南畑 孝介, 後藤 雅宏, 神谷 典穂

    第13回バイオ関連化学シンポジウム  2019.9.4 

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    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Transglutaminase-mediated lipid bilayer decoration with proteins using lipid-fused peptide amphiphile substrates International conference

    Mari Takahara, Rie Wakabayashi, Kosuke Minamihata, Masahiro Goto, Noriho Kamiya

    ACS Fall 2019 National Meeting  2019.8.26 

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    Language：English   Presentation type：Oral presentation (general)  

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  • Enzymatic cell-surface decoration with a functional protein using amphiphilic lipid-fused peptide substrates

    M. Takahara, N. Fujimoto, R. Wakabayash, K. Minamihta, M. Goto, N. Kamiya

    The 99th Annual meeting 2019 of CSJ  2019.3.16 

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    Language：English   Presentation type：Oral presentation (general)  

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  • 細胞表面で機能するタンパク質とその人工合成 Invited

    高原 茉莉

    高分子学会九州支部フォーラム  2019.2.16 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

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  • Design of Amphiphilic Lipid-fused Peptide Substrates for Enzymatic Protein Lipidation International conference

    M. Takahara, R. Wakabayash, K. Minamihta, M. Goto, N. Kamiya

    28th Annual Meeting of MRS-J  2018.12.18 

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    Language：English   Presentation type：Oral presentation (general)  

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  • Chemoenzymatic Synthesis of functional proteins for drug delivery system International conference

    TAKAHARA Mari

    2018.11.16 

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    Language：English   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

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  • Site-specific lipidation of a protein as a drug delivery material

    M. Takahara, R. Wakabayash, K. Minamihta, M. Goto, N. Kamiya

    SCEJ 50th Autumn Meeting  2018.9.18 

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    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Modulation of lipid-protein conjugate using amphiphilic peptide substrates.

    M. Takahara, R. Wakabayash, K. Minamihta, M. Goto, N. Kamiya

    2018.9.9 

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    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Design of lipid-protein conjugate with a self-assembling ability on a cell membrane by using microbial transglutaminase reaction International conference

    M. Takahara, R. Wakabayash, K. Minamihta, M. Goto, N. Kamiya

    256th ACS National Meeting  2018.8.18 

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    Language：English   Presentation type：Oral presentation (general)  

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  • Design of functional nucleic acid aptamers conjugated with antibody Fc domains

    M. Takahara, K. Minamihata, R. Wakabayashi, M. Goto, T. Kusakabe, Jaeman Lee, N. Kamiya

    The 98th Annual Meeting 2018 of CSJ  2018.3.21 

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    Language：English   Presentation type：Oral presentation (general)  

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  • Development of functional DNA aptamer conjugated with constant region of antibody in a site-specific manner

    M. Takahara, K. Minamihata, R. Wakabayashi, M. Goto, T. Kusakabe, Jaeman Lee, N. Kamiya

    SCEJ 83rd annual meeting 2018  2018.3.14 

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    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Transglutaminase-mediated conjugation of DNA-(protein)n polymer using primary amine clustered substrates. International conference

    M. Takahara, R. Wakabayash, K. Minamihta, M. Goto, N. Kamiya

    2017 Kyushu-Seibu/Pusan-Gyeongnam Joint Symposium on High Polymers(18th) and Fibers(16th)  2017.12.15 

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    Language：English   Presentation type：Poster presentation  

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  • 細胞染色を指向した新規DNA-タンパク質複合化法の開発

    高原 茉莉, 若林 里衣, 後藤 雅宏, 神谷 典穂

    日本化学会第97春季年会  2017.3 

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    Language：Japanese   Presentation type：Oral presentation (general)  

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  • 酵素-DNA アプタマー複合体の高効率調製を志向した酵素反応系の構築

    高原茉莉, 林浩之輔, 後藤雅宏, 神谷典穂

    日本化学会第95春季年会  2016.3 

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    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Design of an artificial cellulase with cellulose-binding DNA aptamer International conference

    M. Takahara, Y. Mori, G. A. L Gonçalves, H. Nakazawa, M. Umetsu, N. Kamiya

    The 2015 International Chemical Congress of Pacific Basin Societies (Pacifichem)  2015.12 

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    Language：English   Presentation type：Poster presentation  

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  • Substrate-dependent tailing of DNA aptamers with multiple functional proteins International conference

    M. Takahara, K. Hayashi, M. Goto, N. Kamiya

    The 27th International Symposium on Chemical Engineering  2014.12 

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    Language：English   Presentation type：Oral presentation (general)  

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  • Site-specific conjugation of DNA aptamers and a functional protein by two-step enzymatic reaction International conference

    M. Takahara, K. Hayashi, M. Goto, N. Kamiya

    The 26th International Symposium on Chemical Engineering  2012.12 

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    Language：English   Presentation type：Oral presentation (general)  

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Industrial property rights

  • 脂質化タンパク質の製造方法、及び脂質化タンパク質

    神谷典穂, 若林里衣, 南畑孝介, 高原茉莉

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    Application no：特願2018-051367  Date applied：2018.3.20

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Awards

  • Award for Encouragement of Research in the 29th Annual Meeting of MRS-J

    2020.1   The Materials Research Society of Japan   Cell-surface decoration with lipid-protein conjugates synthesized in an enzymatic manner

    Mari Takahara

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  • 日本化学会第95春季年会 学生講演賞

    2015.4   日本化学会  

    高原 茉莉

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  • 第22回生物工学論文賞

    2014.5   生物工学会  

    M. Takahara, K. Hayashi, M.Goto, N. Kamiya

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  • 第3回生物工学学生優秀賞（飛翔賞）

    2014.5   日本生物工学会  

    高原 茉莉

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Research Projects

  • Development of antigeni modificaiton system to enhance effect of cancer vaccine

    Grant number：21H03825  2021.04 - 2024.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    望月 慎一, 高原 茉莉

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    Grant amount：\17810000 （ Direct expense: \13700000 、 Indirect expense：\4110000 ）

    がんワクチンは生体が自ら持つ免疫機能を活性化してがん細胞を攻撃する治療法であるが、使用されている抗原が自身由来ということもあり、その抗原性は決して高いわけではない。本研究では、がん細胞に特異的に認識される多糖を利用し、がん細胞の抗原性改変に基づく有効ながんワクチンの開発を試みる。
    がん細胞への送達分子としてヒアルロン酸（HA）を選択し、HAと抗原タンパク質のオボアルブミン（OVA）との間の縮合反応によりHA-OVAコンジュゲート体を作製した。作製されたコンジュゲート体を多角度光散乱測定器を備えたゲルろ過クロマトグラフィーで解析したところ、コンジュゲート体はHAとOVAが複数個架橋し、HA1分子と比較して分子量は増加するものの、大きさはほとんど変化ないことが分かった。これは、OVAを架橋点としてHAが折りたたまれ、非常に高密度のHAが作製されたと考えることができる。
    形態が異なるHAとなることで受容体CD44に対する親和性がどのように変化するのか、水晶発振子の振動数変化より評価した。興味深いことにHAよりもコンジュゲート体の方がCD44に強く認識されることが分かった。さらに、OVAに蛍光分子（FITC）を化学修飾し、マウス大腸がん細胞への取り込み量を評価した。OVAはほとんど細胞に取り込まれないのに対し、HA-OVAコンジュゲート体は非常に良く細胞に認識・取り込まれる様子を観察することが出来た。
    取り込まれたOVAはその後、断片化されたペプチドが細胞表面上に提示されると考えられるため、OVA免疫した得られたT細胞と混合培養することで免疫応答が誘導されるか評価する。こうした免疫応答を評価することで、本研究の基本概念となる外来抗原を人為的に送達させることで細胞の抗原性が変化することを証明する。

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  • 酵素反応と薬物融合ペプチドを基盤とした部位特異的な抗体-薬物複合体の開発

    2020.04 - 2021.03

    公益財団法人野口研究所  野口遵研究助成金  エネルギー・資源・環境の革新に寄与する新プロセスや新材料に関する研究

    高原 茉莉

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    Authorship：Principal investigator 

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  • 両親媒性ペプチドによる部位特異的抗体-薬剤複合化技術の開発

    2020.03 - 2021.03

    テルモ生命科学振興財団  研究助成  バイオマテリアル研究

    高原 茉莉

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    Authorship：Principal investigator 

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  • Transglutaminase-mediated lipid bilayer decoration with proteins using lipid-fused peptide amphiphile substrates

    2019.08

    加藤記念バイオサイエンス振興財団  2019年度 国際交流助成（上期） 

    高原 茉莉

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    Authorship：Principal investigator  Grant type：Competitive

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  • 両親媒性化抗体によるイムノリポソーム型抗がん剤の開発

    2019.04 - 2020.03

    池谷科学技術振興財団  単年度研究助成 

    高原 茉莉

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    Authorship：Principal investigator  Grant type：Competitive

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  • 部位特異的脂質化抗体によるイムノリポソーム型抗がん剤の開発

    2019.03 - 2022.04

    科学研究費補助金  若手研究 

    高原 茉莉

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    Authorship：Principal investigator  Grant type：Competitive

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  • 部位特異的脂質修飾技術によるマラリア伝搬阻止ワクチンの開発

    2018.04 - 2020.03

    The Asahi Glass Foundation  Research Grant 

    TAKAHARA Mari

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    Authorship：Principal investigator  Grant type：Competitive

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  • 部位特異的脂質化抗原と核酸からなるリポソームによるがんワクチンの開発

    2017.08 - 2019.03

    科学研究費補助金  研究活動スタート支援 

    高原 茉莉

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    Authorship：Principal investigator  Grant type：Competitive

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  • 固液界面で機能する核酸－酵素ハイブリッド分子の創製とその高度利用

    2014.04 - 2017.03

    科学研究費補助金  特別研究員奨励費 

    高原茉莉

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    Authorship：Principal investigator  Grant type：Competitive

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Class subject in charge

  • Technical English for Interdisciplinary Medical Sciences and Engineering （2024academic year） Late  - その他

  • Research Works for Interdisciplinary Medical Sciences and Engineering （2024academic year） Year-round  - その他

  • Research Works for Interdisciplinary Medical Sciences and Engineering （2024academic year） Year-round  - その他