研究者詳細 - 河野　洋治

論文 - 河野　洋治

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Stem Cell Factors BAM1 and WOX1 Suppressing Longitudinal Cell Division of Margin Cells Evoked by Low-Concentration Auxin in Young Cotyledon of Arabidopsis

Yuli Jiang, Jian Liang, Chunyan Wang, Li Tan, Yoji Kawano, Shingo Nagawa

International Journal of Molecular Sciences 26 ( 10 ) 4724 - 4724 2025年5月

掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

Highly differentiated tissues and organs play essential biological functions in multicellular organisms. Coordination of organ developmental process with tissue differentiation is necessary to achieve proper development of mature organs, but mechanisms for such coordination are not well understood. We used cotyledon margin cells from Arabidopsis plant as a new model system to investigate cell elongation and cell division during organ growth and found that margin cells endured a developmental phase transition from the “elongation” phase to the “elongation and division” phase at the early stage in germinating seedlings. We also discovered that the stem cell factors BARELY ANY MERISTEM 1 (BAM1) and WUSCHEL-related homeobox1 (WOX1) are involved in the regulation of margin cell developmental phase transition. Furthermore, exogenous auxin treatment (1 nanomolar,nM) promotes cell division, especially longitudinal cell division. This promotion of cell division did not occur in bam1 and wox1 mutants. Based on these findings, we hypothesized a new “moderate auxin concentration” model which emphasizes that a moderate auxin concentration is the key to triggering the developmental transition of meristematic cells.

DOI： 10.3390/ijms26104724

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A PRA-Rab trafficking machinery modulates NLR immune receptor plasma membrane microdomain anchoring and blast resistance in rice 査読

Di Liang, Dongyong Yang, Tai Li, Zhe Zhu, Bingxiao Yan, Yang He, Xiaoyuan Li, Keran Zhai, Jiyun Liu, Yoji Kawano, Yiwen Deng, Xu Na Wu, Junzhong Liu, Zuhua He

Science Bulletin 2024年12月

The mutual regulation between the pattern recognition receptor OsCERK1 and the E3 ubiquitin ligase OsCIE1 controls induction and homeostasis of immunity 査読

Qiong Wang, Yoji Kawano

Science Bulletin 2024年7月

An NLR paralog Pit2 generated from tandem duplication of Pit1 fine-tunes Pit1 localization and function. 査読国際誌

Yuying Li, Qiong Wang, Huimin Jia, Kazuya Ishikawa, Ken-Ichi Kosami, Takahiro Ueba, Atsumi Tsujimoto, Miki Yamanaka, Yasuyuki Yabumoto, Daisuke Miki, Eriko Sasaki, Yoichiro Fukao, Masayuki Fujiwara, Takako Kaneko-Kawano, Li Tan, Chojiro Kojima, Rod A Wing, Alfino Sebastian, Hideki Nishimura, Fumi Fukada, Qingfeng Niu, Motoki Shimizu, Kentaro Yoshida, Ryohei Terauchi, Ko Shimamoto, Yoji Kawano

Nature Communications 15 ( 1 ) 4610 - 4610 2024年5月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

NLR family proteins act as intracellular receptors. Gene duplication amplifies the number of NLR genes, and subsequent mutations occasionally provide modifications to the second gene that benefits immunity. However, evolutionary processes after gene duplication and functional relationships between duplicated NLRs remain largely unclear. Here, we report that the rice NLR protein Pit1 is associated with its paralogue Pit2. The two are required for the resistance to rice blast fungus but have different functions: Pit1 induces cell death, while Pit2 competitively suppresses Pit1-mediated cell death. During evolution, the suppression of Pit1 by Pit2 was probably generated through positive selection on two fate-determining residues in the NB-ARC domain of Pit2, which account for functional differences between Pit1 and Pit2. Consequently, Pit2 lost its plasma membrane localization but acquired a new function to interfere with Pit1 in the cytosol. These findings illuminate the evolutionary trajectory of tandemly duplicated NLR genes after gene duplication.

DOI： 10.1038/s41467-024-48943-5

PubMed

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The secreted immune response peptide 1 functions as a phytocytokine in rice immunity 査読

Pingyu Wang, Huimin Jia, Ting Guo, Yuanyuan Zhang, Wanqing Wang, Hideki Nishimura, Zhengguo Li, Yoji Kawano

Journal of Experimental Botany 74 ( 3 ) 1059 - 1073 2023年2月

担当区分：最終著者,　責任著者掲載種別：研究論文（学術雑誌）出版者・発行元：Oxford University Press (OUP)

Abstract

Small signalling peptides play important roles in various plant processes, but information regarding their involvement in plant immunity is limited. We previously identified a novel small secreted protein in rice, called immune response peptide 1 (IRP1). Here, we studied the function of IRP1 in rice immunity. Rice plants overexpressing IRP1 enhanced resistance to the virulent rice blast fungus. Application of synthetic IRP1 to rice suspension cells triggered the expression of IRP1 itself and the defence gene phenylalanine ammonia-lyase 1 (PAL1). RNA-seq results revealed that 84% of genes up-regulated by IRP1, including 13 OsWRKY transcription factors, were also induced by a microbe-associated molecular pattern (MAMP), chitin, indicating that IRP1 and chitin share a similar signalling pathway. Co-treatment with chitin and IRP1 elevated the expression level of PAL1 and OsWRKYs in an additive manner. The increased chitin concentration arrested the induction of IRP1 and PAL1 expression by IRP1, but did not affect IRP1-triggered mitogen-activated protein kinases (MAPKs) activation. Collectively, our findings indicate that IRP1 functions as a phytocytokine in rice immunity regulating MAPKs and OsWRKYs that can amplify chitin and other signalling pathways, and provide new insights into how MAMPs and phytocytokines cooperatively regulate rice immunity.

DOI： 10.1093/jxb/erac455

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その他リンク： https://academic.oup.com/jxb/article-pdf/74/3/1059/49092088/erac455.pdf

Improving disease resistance to rice false smut without yield penalty by manipulating the expression of effector target 査読

Qiong Wang, Yoji Kawano

Molecular Plant 15 ( 12 ) 1834 - 1837 2022年12月

Three highly conserved hydrophobic residues in the predicted α2‐helix of rice NLR protein Pit contribute to its localization and immune induction 査読国際誌

Qiong Wang, Yuying Li, Ken‐ichi Kosami, Chaochao Liu, Jing Li, Dan Zhang, Daisuke Miki, Yoji Kawano

Plant, Cell & Environment 45 ( 6 ) 1876 - 1890 2022年6月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Wiley

Nucleotide-binding leucine-rich repeat (NLR) proteins work as crucial intracellular immune receptors. N-terminal domains of NLRs fall into two groups, coiled-coil (CC) and Toll-interleukin 1 receptor domains, which play critical roles in signal transduction and disease resistance. However, the activation mechanisms of NLRs, and how their N-termini function in immune induction, remain largely unknown. Here, we revealed that the CC domain of a rice NLR Pit contributes to self-association. The Pit CC domain possesses three conserved hydrophobic residues that are known to be involved in oligomer formation in two NLRs, barley MLA10 and Arabidopsis RPM1. Interestingly, the function of these residues in Pit differs from that in MLA10 and RPM1. Although three hydrophobic residues are important for Pit-induced disease resistance against rice blast fungus, they do not participate in self-association or binding to downstream signalling molecules. By homology modelling of Pit using the Arabidopsis ZAR1 structure, we tried to clarify the role of three conserved hydrophobic residues and found that they are located in the predicted α2-helix of the Pit CC domain and involved in the plasma membrane localization. Our findings provide novel insights for understanding the mechanisms of NLR activation as well as the relationship between subcellular localization and immune induction.

DOI： 10.1111/pce.14315

PubMed

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その他リンク： https://onlinelibrary.wiley.com/doi/full-xml/10.1111/pce.14315

Fine-tuning ROS homeostasis by ROD1 is a battleground between rice and Magnaporthe oryzae 査読

Yoji Kawano

Molecular Plant 14 ( 12 ) 1979 - 1981 2021年12月

NB-LRR-encoding genes conferring susceptibility to organophosphate pesticides in sorghumNB-LRR-encoding genes conferring susceptibility to organophosphate pesticides in sorghum 査読

Zihuan Jing, Fiona Wacera W., Tsuneaki Takami, Hideki Takanashi, Fumi Fukada, Yoji Kawano, Hiromi Kajiya-Kanegae, Hiroyoshi Iwata, Nobuhiro Tsutsumi, Wataru Sakamoto

Scientific Reports 11 ( 1 ) 2021年12月

The Small GTPase OsRac1 Forms Two Distinct Immune Receptor Complexes Containing the PRR OsCERK1 and the NLR Pit 査読

Akira Akamatsu, Masayuki Fujiwara, Satoshi Hamada, Megumi Wakabayashi, A i Yao, Qiong Wang, Ken-ichi Kosami, Thu Thi Dang, Takako Kaneko-Kawano, Fumi Fukada, K o Shimamoto, Yoji Kawano

Plant and Cell Physiology 62 ( 11 ) 1662 - 1675 2021年8月

Identification of endogenous small peptides involved in rice immunity through transcriptomics‐ and proteomics‐based screening 査読

Pingyu Wang, Shaolun Yao, Ken‐ichi Kosami, Ting Guo, Jing Li, Yuanyuan Zhang, Yoichiro Fukao, Takako Kaneko‐Kawano, Heng Zhang, Yi‐Min She, Pengcheng Wang, Weiman Xing, Kousuke Hanada, Renyi Liu, Yoji Kawano

Plant Biotechnology Journal 18 ( 2 ) 415 - 428 2020年2月

In vivo monitoring of plant small GTPase activation using a Förster resonance energy transfer biosensor 査読国際誌

Hann Ling Wong, Akira Akamatsu, Qiong Wang, Masayuki Higuchi, Tomonori Matsuda, Jun Okuda, Ken-ichi Kosami, Noriko Inada, Tsutomu Kawasaki, Takako Kaneko-Kawano, Shingo Nagawa, Li Tan, Yoji Kawano, Ko Shimamoto

Plant Methods 14 ( 1 ) 56 - 56 2018年12月

Resistance protein Pit interacts with the GEF OsSPK1 to activate OsRac1 and trigger rice immunity 査読

Qiong Wang, Yuying Li, Kazuya Ishikawa, Ken-ichi Kosami, Kazumi Uno, Shingo Nagawa, Li Tan, Jiamu Du, Ko Shimamoto, Yoji Kawano

Proceedings of the National Academy of Sciences 201813058 2018年11月

The Intronic cis Element SE1 Recruits trans-Acting Repressor Complexes to Repress the Expression of ELONGATED UPPERMOST INTERNODE1 in Rice 査読

Yongyao Xie, Yaling Zhang, Jingluan Han, Jikai Luo, Gousi Li, Jianle Huang, Haibin Wu, Qingwei Tian, Qinlong Zhu, Yuanling Chen, Yoji Kawano, Yao-Guang Liu, Letian Chen

Molecular Plant 11 ( 5 ) 720 - 735 2018年5月

Plasma Membrane Microdomains Are Essential for Rac1-RbohB/H-Mediated Immunity in Rice 査読国際誌

Minoru Nagano, Toshiki Ishikawa, Masayuki Fujiwara, Yoichiro Fukao, Yoji Kawano, Maki Kawai-Yamada, Ko Shimamoto

The Plant Cell 28 ( 8 ) 1966 - 1983 2016年8月

Crosstalk of Signaling Mechanisms Involved in Host Defense and Symbiosis Against Microorganisms in Rice 査読

Akira Akamatsu, Ko Shimamoto, Yoji Kawano

CURRENT GENOMICS 17 ( 4 ) 297 - 307 2016年

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：BENTHAM SCIENCE PUBL LTD

Rice is one of the most important food crops, feeding about half population in the world. Rice pathogens cause enormous damage to rice production worldwide. In plant immunity research, considerable progress has recently been made in our understanding of the molecular mechanisms underlying microbe-associated molecular pattern (MAMP)-triggered immunity. Using genome sequencing and molecular techniques, a number of new MAMPs and their receptors have been identified in the past two decades. Notably, the mechanisms for chitin perception via the lysine motif (LysM) domain-containing receptor OsCERK1, as well as the mechanisms for bacterial MAMP (e.g. flg22, elf18) perception via the leucine-rich repeat (LRR) domain-containing receptors FLS2 and EFR, have been clarified in rice and Arabidopsis, respectively. In chitin signaling in rice, two direct substrates of OsCERK1, Rac/ROP GTPase guanine nucleotide exchange factor OsRacGEF1 and receptor-like cytoplasmic kinase OsRLCK185, have been identified as components of the OsCERK1 complex and are rapidly phosphorylated by OsCERK1 in response to chitin. Interestingly, OsCERK1 also participates in symbiosis with arbuscular mycorrhizal fungi (AMF) in rice and plays a role in the recognition of short-chitin molecules (CO4/5), which are symbiotic signatures included in AMF germinated spore exudates and induced by synthetic strigolactone. Thus, OsCERK1 contributes to both immunity and symbiotic responses. In this review, we describe recent studies on pathways involved in rice immunity and symbiotic signaling triggered by interactions with microorganisms. In addition, we describe recent advances in genetic engineering by using plant immune receptors and symbiotic microorganisms to enhance disease resistance of rice.

DOI： 10.2174/1389202917666160331201602

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The RhoGAP SPIN6 Associates with SPL11 and OsRac1 and Negatively Regulates Programmed Cell Death and Innate Immunity in Rice 査読

Jinling Liu, Chan Ho Park, Feng He, Minoru Nagano, Mo Wang, Maria Bellizzi, Kai Zhang, Xiaoshan Zeng, Wende Liu, Yuese Ning, Yoji Kawano, Guo-Liang Wang

PLOS PATHOGENS 11 ( 2 ) 2015年2月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：PUBLIC LIBRARY SCIENCE

The ubiquitin proteasome system in plants plays important roles in plant-microbe interactions and in immune responses to pathogens. We previously demonstrated that the rice U-box E3 ligase SPL11 and its Arabidopsis ortholog PUB13 negatively regulate programmed cell death (PCD) and defense response. However, the components involved in the SPL11/PUB13-mediated PCD and immune signaling pathway remain unknown. In this study, we report that SPL11-interacting Protein 6 (SPIN6) is a Rho GTPase-activating protein (RhoGAP) that interacts with SPL11 in vitro and in vivo. SPL11 ubiquitinates SPIN6 in vitro and degrades SPIN6 in vivo via the 26S proteasome-dependent pathway. Both RNAi silencing in transgenic rice and knockout of Spin6 in a T-DNA insertion mutant lead to PCD and increased resistance to the rice blast pathogen Magnaporthe oryzae and the bacterial blight pathogen Xanthomonas oryzae pv. oryzae. The levels of reactive oxygen species and defense-related gene expression are significantly elevated in both the Spin6 RNAi and mutant plants. Strikingly, SPIN6 interacts with the small GTPase OsRac1, catalyze the GTP-bound OsRac1 into the GDP-bound state in vitro and has GAP activity towards OsRac1 in rice cells. Together, our results demonstrate that the RhoGAP SPIN6 acts as a linkage between a U-box E3 ligase-mediated ubiquitination pathway and a small GTPase-associated defensome system for plant immunity.

DOI： 10.1371/journal.ppat.1004629

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New insights into the dimerization of small GTPase Rac/ROP guanine nucleotide exchange factors in rice. 査読国際誌

Akamatsu Akira, Uno Kazumi, Kato Midori, Wong Hann Ling, Shimamoto Ko, Kawano Yoji

Plant signaling & behavior 10 ( 7 ) e1044702 2015年

The Crystal Structure of the Plant Small GTPase OsRac1 Reveals Its Mode of Binding to NADPH Oxidase 査読

Ken-ichi Kosami, Izuru Ohki, Minoru Nagano, Kyoko Furuita, Toshihiko Sugiki, Yoji Kawano, Tsutomu Kawasaki, Toshimichi Fujiwara, Atsushi Nakagawa, Ko Shimamoto, Chojiro Kojima

JOURNAL OF BIOLOGICAL CHEMISTRY 289 ( 41 ) 28569 - 28578 2014年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

Rac/Rop proteins are Rho-type small GTPases that act as molecular switches in plants. Recent studies have identified these proteins as key components in many major plant signaling pathways, such as innate immunity, pollen tube growth, and root hair formation. In rice, the Rac/Rop protein OsRac1 plays an important role in regulating the production of reactive oxygen species (ROS) by the NADPH oxidase OsRbohB during innate immunity. However, the molecular mechanism by which OsRac1 regulates OsRbohB remains unknown. Here, we report the crystal structure of OsRac1 complexed with the non-hydrolyzable GTP analog guanosine 5'-(beta,gamma-imido) triphosphate at 1.9 angstrom resolution; this represents the first active-form structure of a plant small GTPase. To elucidate the ROS production in rice cells, structural information was used to design OsRac1 mutants that displayed reduced binding to OsRbohB. Only mutations in the OsRac1 Switch I region showed attenuated interactions with OsRbohB in vitro. In particular, Tyr(39) and Asp(45) substitutions suppressed ROS production in rice cells, indicating that these residues are critical for interaction with and activation of OsRbohB. Structural comparison of active-form OsRac1 with AtRop9 in its GDP-bound inactive form showed a large conformational difference in the vicinity of these residues. Our results provide new insights into the molecular mechanism of the immune response through OsRac1 and the various cellular responses associated with plant Rac/Rop proteins.

DOI： 10.1074/jbc.M114.603282

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Rho family GTPase-dependent immunity in plants and animals 査読

Yoji Kawano, Takako Kaneko-Kawano, Ko Shimamoto

FRONTIERS IN PLANT SCIENCE 5 2014年10月

担当区分：筆頭著者,　責任著者記述言語：英語出版者・発行元：FRONTIERS RESEARCH FOUNDATION

In plants, sophisticated forms of immune systems have developed to cope with a variety of pathogens. Accumulating evidence indicates that Rac (also known as Rop), a member of the Rho family of small GTPases, is a key regulator of immunity in plants and animals. Like other small GTPases, Rac/Rop GTPases function as a molecular switch downstream of immune receptors by cycling between GDP-bound inactive and GTP-bound active forms in cells. Rac/Rop GTPases trigger various immune responses, thereby resulting in enhanced disease resistance to pathogens. In this review, we highlight recent studies that have contributed to our current understanding of the Rac/Rop family GTPases and the upstream and downstream proteins involved in plant immunity. We also compare the features of effector-triggered immunity between plants and animals, and discuss the in vivo monitoring of Rac/Rop activation.

DOI： 10.3389/fpls.2014.00522

Web of Science

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