Faculty Profiles - NAKANO Keisuke

Papers - NAKANO Keisuke

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Histopathological study of matrix mineralization by osteoblastic-like and odontoblastic-like cells in diffusion chamber. Reviewed

Rodriguez AP, Borkosky SS, Han PP, Tsujigiwa H, Takagi T, Sathi GS, Lee YJ, Katase N, Okauchi M, Nakano K, Xiao J

J Hard Tissue Biol. 15 ( 1 ) 6 - 10 2006.1

Expression and localization of cartilage-specific matrix protein chondromodulin-I mRNA in salivary pleomorphic adenomas. International journal

Kimihide Kusafuka, Keisuke Nakano, Yuji Hiraki, Chisa Shukunami, Hitoshi Nagatsuka, Noriyuki Nagai, Tamiko Takemura, Yutaku Sakaguchi, Kazuichi Okazaki, Michi Kusafuka, Hiroko Hisha, Susumu Ikehara

Virchows Archiv : an international journal of pathology 446 ( 1 ) 34 - 40 2005.1

Language：English Publishing type：Research paper (scientific journal)

Pleomorphic adenoma is the most common epithelial tumor in the salivary glands. This tumor frequently exhibits "mesenchyme"-like components, including myxoid or chondroid areas. Recently, using immunohistochemical techniques, we reported that cartilage-specific matrix protein, chondromodulin-I (ChM-I), was deposited on the inter-territorial matrix of the chondroid area in salivary pleomorphic adenomas and that ChM-I, which is also a strong angio-inhibitory factor, plays an important role in the avascular nature of the chondroid area and the chondroid formation in this type of tumor. To elucidate which cells express ChM-I mRNA in pleomorphic adenomas, we examined the expression and localization of ChM-I mRNA in this type of tumor using an in situ hybridization technique. Immunoreactivity for ChM-I was observed in the inter-territorial matrix of the chondroid area, especially around the lacunae, and in the cytoplasm of neoplastic myoepithelial cells of the myxoid element of pleomorphic adenomas. On in situ hybridization analysis, strong signals for ChM-I mRNA were detected in the cytoplasm of the lacuna cells of the chondroid element, and moderate to marked signals were observed in the cytoplasm of the neoplastic myoepithelial cells of the myxoid element. Signals for ChM-I mRNA were also seen in the cytoplasm of the spindle-shaped neoplastic myoepithelial cells in the transitional areas between the myxoid and chondroid elements of this tumor. Signals for ChM-I mRNA were not seen in the inner ductal cells or the fibrous element. These findings indicate that lacuna cells and neoplastic myoepithelial cells express ChM-I mRNA and that mature ChM-I, which lacuna cells and neoplastic myoepithelial cells translate, is deposited in the chondroid matrix of pleomorphic adenomas. In conclusion, lacuna cells and neoplastic myoepithelial cells express ChM-I mRNA ectopically in pleomorphic adenoma, and this plays an important role in chondroid formation and hypovascularity in this type of tumor.

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Elemental sulphur and alkali elutable melanin detected in oral melanosis and malignant melanoma by energy-filtering transmission electron microscopy. Reviewed International journal

Noriyuki Nagai, You-Jin Lee, Noriyuki Nagaoka, Mehmet Gunduz, Keisuke Nakano, Tetsundo Nojima, Hidetsugu Tsujigiwa, Esra Gunduz, Chong Huat Siar, Hitoshi Nagatsuka

Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 31 ( 8 ) 481 - 7 2002.9

Distribution of basement membrane type IV collagen alpha chains in ameloblastoma: an immunofluorescence study. International journal

Keisuke Nakano, Chong Huat Siar, Noriyuki Nagai, Ichiro Naito, Yoshikazu Sado, Hitoshi Nagatsuka, Choufuku Hoh, Katsuhiro Kurada, Hidetsugu Tsujigiwa, Mehmet Gunduz

Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 31 ( 8 ) 494 - 9 2002.9

Language：English Publishing type：Research paper (scientific journal)

BACKGROUND: Type IV collagen, a heterotrimeric molecule that exists in six genetically distinct forms, alpha1(IV)-alpha6(IV) is a major structural component of basement membrane (BM) and acts as a scaffold for other BM constituents. METHODS: Indirect immunofluorescence using alpha chain-specific monoclonal antibodies was employed to clarify basement membrane (BM) collagen IV distribution in two ameloblastoma, and for comparison, on oral mucosa and tooth germ. RESULTS: Ameloblastoma BM expressed five of six genetically distinct forms of collagen IV: alpha1(IV), alpha2(IV), alpha5(IV) and alpha6(IV) chains occurred as intense linear stainings without disruption around neoplastic epithelium, and this expression pattern was fundamentally similar to oral mucosa BM; alpha4(IV) expression was rare and occurred around nests of primitive tumor cells or potentially invasive sites. The tooth germ demonstrated a stage- and position-specific collagen IV distribution: the inner enamel epithelium BM expressed alpha1(IV), alpha2(IV), and alpha4(IV) except in the cuspal predentine region; and the outer enamel epithelium BM expressed alpha1(IV), alpha2(IV), alpha5(IV), and alpha6(IV) chains. CONCLUSIONS: Results suggest that collagen IV alpha chain distribution in ameloblastoma BM plays an important role in tumor cytodifferentiation and progression.

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Localization of type IV collagen a 1 to a 6 chains in basement membrane during mouse molar germ development. Reviewed

Nagai N, Nakano K, Sado Y, Naito I, Gunduz M, Tsujigiwa H, Nagatsuka H, Ninomiya Y, Siar CH

The International journal of developmental biology 45 ( 7 ) 827 - 831 2001.10

Experimental Study on Bone Formation by Porous HAP-BMP Composite in Bone Defect with Osteointegrated Implant

OGATA Kazumasa, TAN Jun, OGATA Tetsurou, KOHNO Watari, HARUTSUGI Kentarou, KANOU Kunihiro, NAKANO Keisuke, KINUTA Yoshihiro, INOUE Masahisa

Journal of Japanese Society of Oral Implantology 10 ( 3 ) 272 - 279 1997.9

Language：Japanese Publisher：Japanese Society of Oral Implantology

Hydroxyapatite (HAP) is considered one of the best bioactive ceramics because of high bone conduction/induction in osteogenic tissue. Although it has several characteristics of an ideal bone substitute, it does not have bone induction in heterogenous tissue. On the other hand, partially-purified bone morphogenetic protein (BMP), which contains BMP-2 and can induce differentiation of mesenchymal cells into osteoblast, was extracted from bovine bone matrix. In this study, porous HAP-BMP complex was inserted into the bone defect in case of HAP-coated implant, an integral implant system for the dog mandible.<br/> The results showed significant increases in the height and the area of the new bone in the porous HAP-BMP complex experimental group as compared with the control group. From the results above, it was considered that porous HAP-BMP composite maintains the thickness of regenerated tissue of the bone defect around implant.

DOI： 10.11237/jsoi.10.272

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Characterization of Bovine extracted BMP and development of Bone Collagen I Carrier

NAGAI Noriyuki, MURATA Masaru, TSUJIGIWA Hidetsugu, INOUE Masahisa, NAGATSUKA Hitoshi, NAKANO Keisuke, AKAGI Takumi, KINUTA Yoshihiro, KANYAMA Manabu, QIN Chun-Lin, ZHANG Shao-Quan, KONOUCHI Hironobu, HAN Song, HUANG Bing-Zhen, TAN Jun, LIU Gui-Ru, GOMAH Atia, HIBI Kazumitsu

Journal of hard tissue biology 5 ( 2 ) 163 - 173 1996.9

Language：Japanese Publisher：日本硬組織研究技術学会

A modofied method was established to extracted BMP from bovine bone. SDS-PAGE and Westen blotting were apllied to analize the components of this partially purified BMP. In addition the amino acid sequence was studied using protein sequencer to analyze the unknown bands. Type I collagen derived from bone was used as a carrier whose properties and advantages were studied. The following conclusions are made from this study. 1. The limitation of molecluar weight in tbe early step of BMP purification procedure improves purification efficiency and stability, which makes the applicadon of BMP in big animalos possible. 2. In this method the purified BMP product also contains histone H3, histone 2B and other unknown proteins as well as BMP-2. 3. The atelocollagen derived from bone has proved to be an useful carrier for BMP. This carrier shows characteristics of self absorption and recalcification when embedded in tissues. 4. The minimun bone induction dose of BMP at each step (G-Ext:500μg, Hep:30μg, S200:50μg) and the optimal bone induction dose(G-Ext:5.0mg, Hep:2.0mg, S200:0.3mg) are determined, which makes the applicalion of BMP in cellular biology possible.

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アパタイト : 骨結合界面の初期石灰化とBMPによる異所性骨誘導

永井教之, 長塚仁, 井上正久, 村田勝, 赤木巧, 辻極秀次, 中野敬介, 馬渕優, 寒河江登志朗, 高木亨, 久保木芳徳

Journal of hard tissue biology = 日本硬組織研究技術学会雑誌 4 ( 3 ) 79 - 90 1995.11

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