研究者詳細 - 渡邊　彰吾

Antioxidant action of xanthine oxidase inhibitor febuxostat protects the liver and blood vasculature in SHRSP5/Dmcr rats. 査読国際誌

Mai Kakimoto, Moe Fujii, Ikumi Sato, Koki Honma, Hinako Nakayama, Sora Kirihara, Taketo Fukuoka, Shang Ran, Satoshi Hirohata, Kazuya Kitamori, Shusei Yamamoto, Shogo Watanabe

Journal of applied biomedicine 21 ( 2 ) 80 - 90 2023年6月

記述言語：英語掲載種別：研究論文（学術雑誌）

BACKGROUND: Xanthine oxidase (XO) generates reactive oxygen species during uric acid production. Therefore, XO inhibitors, which suppress oxidative stress, may effectively treat non-alcoholic steatohepatitis (NASH) and atherosclerosis via uric acid reduction. In this study, we examined the antioxidant effect of the XO inhibitor febuxostat on NASH and atherosclerosis in stroke-prone spontaneously hypertensive 5 (SHRSP5/Dmcr) rats. METHODS: SHRSP5/Dmcr rats were divided into three groups: SHRSP5/Dmcr + high-fat and high-cholesterol (HFC) diet [control group, n = 5], SHRSP5/Dmcr + HFC diet + 10% fructose (40 ml/day) [fructose group, n = 5], and SHRSP5/Dmcr + HFC diet + 10% fructose (40 ml/day) + febuxostat (1.0 mg/kg/day) [febuxostat group, n = 5]. Glucose and insulin resistance, blood biochemistry, histopathological staining, endothelial function, and oxidative stress markers were evaluated. RESULTS: Febuxostat reduced the plasma uric acid levels. Oxidative stress-related genes were downregulated, whereas antioxidant factor-related genes were upregulated in the febuxostat group compared with those in the fructose group. Febuxostat also ameliorated inflammation, fibrosis, and lipid accumulation in the liver. Mesenteric lipid deposition decreased in the arteries, and aortic endothelial function improved in the febuxostat group. CONCLUSIONS: Overall, the XO inhibitor febuxostat exerted protective effects against NASH and atherosclerosis in SHRSP5/Dmcr rats.

DOI： 10.32725/jab.2023.009

Therapeutic effect of ouabagenin, a novel liver X receptor agonist, on atherosclerosis in nonalcoholic steatohepatitis in SHRSP5/Dmcr rat model 査読

Shusei Yamamoto, Ikumi Sato, Moe Fujii, Mai Kakimoto, Koki Honma, Sora Kirihara, Hinako Nakayama, Taketo Fukuoka, Satoru Tamura, Minoru Ueda, Satoshi Hirohata, Shogo Watanabe

Canadian Journal of Physiology and Pharmacology 2023年5月

掲載種別：研究論文（学術雑誌）出版者・発行元：Canadian Science Publishing

The liver X receptor (LXR) can enhance cholesterol transporters, which could remove excess cholesterol from foam cells in atheromas. LXR has two subtypes: LXRα, which aggravates hepatic lipid accumulation, and LXRβ, which does not. In 2018, ouabagenin (OBG) was reported as a potential LXRβ-specific agonist. We aimed to examine whether OBG specifically affects LXRβ in nonalcoholic steatohepatitis (NASH); it did not aggravate hepatic steatosis and can suppress the development of atherosclerosis. SHRSP5/Dmcr rats fed a high-fat and high-cholesterol diet were divided into four groups as follows: (I) L-NAME group, (II) L-NAME/OBG group, (III) OBG (－) group, and (IV) OBG (+) group. All groups’ rats were intraperitoneally administered L-NAME. The L-NAME/OBG groups’ rats were intraperitoneally administered OBG and L-NAME simultaneously. After L-NAME administration, the OBG (+) groups’ rats were administered OBG, while the OBG (－) groups’ rats were not. Although all rats developed NASH, OBG did not exacerbate steatosis (L-NAME/OBG and OBG (+) groups). In addition, endothelial cells were protected in the L-NAME/OBG group and foam cells in the atheroma were reduced in the OBG (+) group. OBG is an LXRβ-specific agonist and has a potential therapeutic effect on atherosclerosis without developing lipid accumulation in the liver.

DOI： 10.1139/cjpp-2022-0532

SHRSP5/Dmcr rats fed a high-fat and high-cholesterol diet develop disease-induced sarcopenia as nonalcoholic steatohepatitis progresses. 査読国際誌

Shusei Yamamoto, Koki Honma, Moe Fujii, Mai Kakimoto, Sora Kirihara, Hinako Nakayama, Kazuya Kitamori, Ikumi Sato, Satoshi Hirohata, Shogo Watanabe

Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft 152104 - 152104 2023年5月

記述言語：英語掲載種別：研究論文（学術雑誌）

BACKGROUND: Secondary sarcopenia develops as a result of a bedridden state and illnesses, such as cachexia, liver disease, and diabetes. However, there is a lack of animal models to investigate the underlying mechanisms and potential treatments for secondary sarcopenia. Recently, secondary sarcopenia has been associated with the prognosis of nonalcoholic steatohepatitis. This study aimed to investigate whether stroke-prone spontaneously hypertensive rat 5 (SHRSP5/Dmcr) which developed severe nonalcoholic steatohepatitis by a high-fat and high-cholesterol (HFC; containing 2% cholic acid) diet is a useful model of secondary sarcopenia. METHODS: SHRSP5/Dmcr rats were divided into 6 groups fed with a Stroke-Prone (SP: normal chow) or HFC diets for different periods (4, 12, and 20 weeks), and WKY/Izm rats were divided into 2 groups fed an SP or HFC diet. Body weight, food intake, and muscle force were measured weekly for all rats. After the end of the diet period, skeletal muscle strength evoked by electrical stimulation was recorded, blood was collected, and organ weight was measured. The sera were used for biochemical analysis and the organs were used for histopathological analysis. RESULTS: SHRSP5/Dmcr rats fed an HFC diet developed nonalcoholic steatohepatitis, and their skeletal muscles, especially fast muscles, showed atrophy, indicating that muscle atrophy is aggravated by the progression of nonalcoholic steatohepatitis. In contrast, WKY/Izm rats fed an HFC diet did not exhibit sarcopenia. CONCLUSIONS: This study suggests that SHRSP5/Dmcr rats could be a useful novel model for investigate the mechanism of secondary sarcopenia disorder associated with nonalcoholic steatohepatitis.

DOI： 10.1016/j.aanat.2023.152104

高脂肪食が腸内細菌叢および肝臓NLRP3インフラマソームに与える影響査読

中山日菜子, 桐原空, 福岡威人, 本間宏基, 藤井萌, 廣畑聡, 山元修成, 渡辺彰吾

腸内細菌学雑誌 37 ( 2 ) 104 - 104 2023年4月

高脂肪食誘導性NASH動物モデルにおける腸内細菌叢とLeaky gutの評価査読

桐原空, 中山日菜子, 福岡威人, 本間宏基, 藤井萌, 廣畑聡, 山元修成, 渡辺彰吾

腸内細菌学雑誌 37 ( 2 ) 103 - 103 2023年4月

Increased Glycine-conjugated and Unconjugated Bile Acid Levels Associated with Aggravation of Nonalcoholic Steatohepatitis and Cardiovascular Disease in SHRSP5/Dmcr Rat. 査読

Shusei Yamamoto, Ikumi Sato, Moe Fujii, Mai Kakimoto, Koki Honma, Natsumi Akiyama, Miku Sakai, Natsuki Fukuhama, Shota Kumazaki, Satoshi Hirohata, Kazuya Kitamori, Yukio Yamori, Shogo Watanabe

Acta medica Okayama 77 ( 1 ) 29 - 36 2023年2月

記述言語：英語掲載種別：研究論文（学術雑誌）

The SHRSP5/Dmcr is a useful animal model for the development of nonalcoholic steatohepatitis (NASH) pathology when fed a high-fat, high-cholesterol diet, and further drug interventions can lead to concomitant cardiovascular disease. While SHRSP5/Dmcr rats have been used for basic research related to NASH, details of their bile acid metabolism in this condition are unknown. In this study, we aimed to clarify the changes in the serum bile acid (BA) fractions associated with NASH and found that glycine-conjugated and unconjugated bile acid increased with worsening NASH and cardiovascular disease while taurine-conjugated BA relatively decreased.

DOI： 10.18926/AMO/64358

Uric acid elevation by fructose overload exacerbates NASH and atherosclerosis via oxidative stress 査読

Fujii M, Kakimoto M, Sato I, Honma K, Kirihara S, Nakayama H, Fukuoka T, Hirohata S, Kitamori K, Ran S, Yamamoto S, Watanabe S

Current nutrition and food science 2023年

非アルコール性脂肪肝炎モデル動物であるSHRSP5/Dmcrラットは二次性サルコペニアを発症する査読

山元修成, 本間宏基, 藤井萌, 柿本麻衣, 桐原空, 中山日菜子, 佐藤生弥, 廣畑聡, 渡辺彰吾

日本サルコペニア・フレイル学会雑誌 6 ( Suppl. ) 180 - 180 2022年10月

Suppression of nitric oxide synthase aggravates non-alcoholic steatohepatitis and atherosclerosis in SHRSP5/Dmcr rat via acceleration of abnormal lipid metabolism. 査読国際誌

Ikumi Sato, Shusei Yamamoto, Mai Kakimoto, Moe Fujii, Koki Honma, Shota Kumazaki, Mami Matsui, Hinako Nakayama, Sora Kirihara, Shang Ran, Shinichi Usui, Ryoko Shinohata, Kazuya Kitamori, Satoshi Hirohata, Shogo Watanabe

Pharmacological reports : PR 74 ( 4 ) 669 - 683 2022年7月

記述言語：英語掲載種別：研究論文（学術雑誌）

BACKGROUND: Non-alcoholic steatohepatitis (NASH) is a progressive subtype of non-alcoholic fatty liver disease (NAFLD) that is closely related to cardiovascular disease (CVD). Nitric oxide (NO) plays a critical role in the control of various biological processes. Dysfunction of the NO signaling pathway is associated with various diseases such as atherosclerosis, vascular inflammatory disease, and diabetes. Recently, it has been reported that NO is related to lipid and cholesterol metabolism. Chronic NO synthase (NOS) inhibition accelerates NAFLD by increasing hepatic lipid deposition. However, the detailed relationship between NO and abnormal lipid and cholesterol metabolism in NAFLD/NASH has not been completely explained. We aimed to determine the effects of NOS inhibition by N omega-nitro-L-arginine methyl ester hydrochloride (L-NAME), a NOS inhibitor, on NASH and CVD via lipid and cholesterol metabolism. METHODS: Stroke-prone spontaneously hypertensive rats were fed a high-fat and high-cholesterol diet for 8 weeks and administered L-NAME for the last 2 weeks. Following blood and tissue sampling, biochemical analysis, histopathological staining, quantitative RT-PCR analysis, and western blotting were performed. RESULTS: L-NAME markedly increased hepatic triglyceride (TG) and cholesterol levels by promoting TG synthesis and cholesterol absorption from the diet. L-NAME increased the mRNA levels of inflammatory markers and fibrotic areas in the liver. Cholesterol secretion from the liver was promoted in rats administered L-NAME, which increased serum cholesterol. L-NAME significantly increased the level of oxidative stress marker and lipid deposition in the arteries. CONCLUSIONS: NOS inhibition simultaneously aggravates NASH and atherosclerosis via hepatic lipid and cholesterol metabolism.

DOI： 10.1007/s43440-022-00380-1

Potential of a Novel Chemical Compound Targeting Matrix Metalloprotease-13 for Early Osteoarthritis: An In Vitro Study. 査読国際誌

Junko Inagaki, Airi Nakano, Omer Faruk Hatipoglu, Yuka Ooka, Yurina Tani, Akane Miki, Kentaro Ikemura, Gabriel Opoku, Ryosuke Ando, Shintaro Kodama, Takashi Ohtsuki, Hirosuke Yamaji, Shusei Yamamoto, Eri Katsuyama, Shogo Watanabe, Satoshi Hirohata

International journal of molecular sciences 23 ( 5 ) 2022年2月

記述言語：英語掲載種別：研究論文（学術雑誌）

Osteoarthritis is a progressive disease characterized by cartilage destruction in the joints. Matrix metalloproteinases (MMPs) and a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTSs) play key roles in osteoarthritis progression. In this study, we screened a chemical compound library to identify new drug candidates that target MMP and ADAMTS using a cytokine-stimulated OUMS-27 chondrosarcoma cells. By screening PCR-based mRNA expression, we selected 2-(8-methoxy-2-methyl-4-oxoquinolin-1(4H)-yl)-N-(3-methoxyphenyl) acetamide as a potential candidate. We found that 2-(8-methoxy-2-methyl-4-oxoquinolin-1(4H)-yl)-N-(3-methoxyphenyl) acetamide attenuated IL-1β-induced MMP13 mRNA expression in a dose-dependent manner, without causing serious cytotoxicity. Signaling pathway analysis revealed that 2-(8-methoxy-2-methyl-4-oxoquinolin-1(4H)-yl)-N-(3-methoxyphenyl) acetamide attenuated ERK- and p-38-phosphorylation as well as JNK phosphorylation. We then examined the additive effect of 2-(8-methoxy-2-methyl-4-oxoquinolin-1(4H)-yl)-N-(3-methoxyphenyl) acetamide in combination with low-dose betamethasone on IL-1β-stimulated cells. Combined treatment with 2-(8-methoxy-2-methyl-4-oxoquinolin-1(4H)-yl)-N-(3-methoxyphenyl) acetamide and betamethasone significantly attenuated MMP13 and ADAMTS9 mRNA expression. In conclusion, we identified a potential compound of interest that may help attenuate matrix-degrading enzymes in the early osteoarthritis-affected joints.

DOI： 10.3390/ijms23052681

Basic characteristics between mechanomyogram and muscle force during twitch and tetanic contractions in rat skeletal muscles 査読

Ikumi Sato, Shusei Yamamoto, Mai Kakimoto, Moe Fujii, Koki Honma, Shota Kumazaki, Mami Matsui, Hinako Nakayama, Sora Kirihara, Shang Ran, Satoshi Hirohata, Shogo Watanabe

Journal of Electromyography and Kinesiology 62 ( 102627 ) 2022年1月

A high-fat/high-cholesterol diet, but not high-cholesterol alone, increases free cholesterol and apoE-rich HDL serum levels in rats and upregulates hepatic ABCA1 expression 査読

Biochimie 2022年1月

Glycine-conjugated and Unconjugated Bile Acid Levels Increased in SHRSP5/Dmcr Rat with Aggravation of Nonalcoholic Steatohepatitis and Cardiovascular Disease 査読

Shusei Yamamoto, Ikumi Sato, Moe Fujii, Mai Kakimoto, Koki Honma, Natsumi Akiyama, Miku Sakai, Natsuki Fukuhama, Shota Kumazaki, Satoshi Hirohata, Kazuya Kitamori, Yukio Yamori, Shogo Watanabe

Acta Medica Okayama 2022年

Osteopontin silencing attenuates bleomycin-induced murine pulmonary fibrosis by regulating epithelial?mesenchymal transition 査読国際誌

Biomedicine & Pharmacotherapy 139 ( 11 ) 111633 - 111633 2021年7月

ポータブル腸電位計と腹部エコーを用いた腸蠕動の可視化と看護実践のイノベーション招待査読

修文大学紀要 12 1 - 13 2021年3月

Low plasma apolipoprotein E-rich high-density lipoprotein levels in patients with metabolic syndrome. 査読国際誌

Ryoko Shinohata, Yuhei Shiga, Shin-Ichiro Miura, Satoshi Hirohata, Misako Shibakura, Tomoe Ueno-Iio, Shogo Watanabe, Yujiro Arao, Shinichi Usui

Clinica chimica acta; international journal of clinical chemistry 510 531 - 536 2020年11月

記述言語：英語掲載種別：研究論文（学術雑誌）

BACKGROUND: High-density lipoprotein (HDL) containing apolipoprotein E (apoE-rich HDL) represents a small portion of plasma HDL. We recently established a method for measuring plasma apoE-rich HDL. This study aimed to investigate the relationship between metabolic syndrome (MetS) and apoE-rich HDL levels. METHODS: The apoE-rich HDL-cholesterol (HDL-C) levels and metabolic characteristics of 113 patients were analyzed. RESULTS: The MetS group (n = 58) had significantly lower apoE-rich HDL-C and a lower apoE-rich HDL-C/HDL-C ratio (apoE-HDL (%)) compared to the non-MetS group. The prevalence of MetS was increased when apoE-HDL (%) decreased. In simple regression analyses, apoE-HDL (%) was significantly inversely correlated with visceral fat area (rs = -0.370, P < 0.001) and plasma triglycerides (rs = -0.447, P < 0.001) and positively correlated with low-density lipoprotein (LDL) mean particle size (rs = 0.599, P < 0.001) and HDL mean particle size (rs = 0.512, P < 0.001). Stepwise multiple regression analysis revealed that LDL mean particle size, a component of the atherogenic lipoprotein profile, was an independent predictor of apoE-HDL (%) (adjusted R2 = 0.409). CONCLUSIONS: Plasma apoE-rich HDL levels might be a valuable indicator of MetS. These findings may help further understand HDL subfraction analysis in cardiometabolic diseases.

DOI： 10.1016/j.cca.2020.08.020

Clinical importance of respiratory muscle fatigue in patients with cardiovascular disease 査読

Taya, M., Amiya, E., Hatano, M., Saito, A., Nitta, D., Maki, H., Hosoya, Y., Minatsuki, S., Tsuji, M., Sato, T., Murakami, H., Narita, K., Konishi, Y., Watanabe, S., Yokota, K., Haga, N. & Komuro, I

Medicine 99 ( 34 ) e21794 2020年8月

Low plasma apolipoprotein E-rich high-density lipoprotein levels in patients with metabolic syndrome 査読

Ryoko Shinohata,Yuhei Shiga,Shin-Ichiro Miura,Satoshi Hirohata,Misako Shibakura,Tomoe Ueno-Iio,Shogo Watanabe,Yujiro Arao,Shinichi Usui

Clinica Chimica Acta 510 531 - 536 2020年8月

Bile acids aggravate nonalcoholic steatohepatitis and cardiovascular disease in SHRSP5/Dmcr rat model 査読国際誌

Experimental and Molecular Pathology 114 104437 - 104437 2020年6月

Bile acid metabolism is an intermediary factor between non-alcoholic steatohepatitis and ischemic heart disease in SHRSP5/Dmcr rats 査読

Journal of Nutrition and Food Sciences 09 ( 04 ) 2019年10月

Non-alcoholic steatohepatitis aggravates nitric oxide synthase inhibition-induced arteriosclerosis in SHRSP5/Dmcr rat model 査読

Shogo Watanabe, Shota Kumazaki, Shusei Yamamoto, Ikumi Sato, Kazuya Kitamori, Mari Mori, Yukio Yamori, Satoshi Hirohata

International Journal of Experimental Pathology 2019年1月

Non-alcoholic steatohepatitis aggravates nitric oxide synthase inhibition-induced arteriosclerosis in SHRSP5/Dmcr rat model. 査読国際誌

Shogo Watanabe, Shota Kumazaki, Shusei Yamamoto, Ikumi Sato, Kazuya Kitamori, Mari Mori, Yukio Yamori, Satoshi Hirohata

International journal of experimental pathology 99 ( 6 ) 282 - 294 2018年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

Non-alcoholic steatohepatitis (NASH) is linked to increased cardiovascular risk, independent of the broad spectrum of metabolic syndrome risk factors. Stroke-prone (SP) spontaneously hypertensive rats (SHRSP5/Dmcr) fed a high-fat and high-cholesterol (HFC) diet developed hepatic lesions similar to those in human NASH pathology. These rats simultaneously developed lipid deposits in the mesenteric arteries, cardiac fibrosis, endothelial dysfunction and left ventricle (LV) diastolic dysfunction. However, the intermediary factors between NASH and cardiovascular disease are still unknown. We investigated whether NASH aggravates nitric oxide (NO) synthase inhibition-induced arteriosclerosis in SHRSP5/Dmcr rats. Wistar Kyoto and SHRSP5/Dmcr rats were divided into 4 groups of 5 and fed the stroke-prone (SP) or HFC diets for 8 weeks. To induce NO synthase inhibition, Nω -nitro-L-arginine methyl ester hydrochloride (L-NAME) mixed with drinking water was administered in the final 2 weeks. The NASH+L-NAME group demonstrated the following characteristics related to arteriosclerosis and myocardial ischaemia: (a) LV systolic dysfunction with asynergy, (b) replacement fibrosis caused by the shedding of cardiomyocytes and (c) arterial lipid deposition and coronary occlusion secondary to endothelial dysfunction. These characteristics were not observed in the NASH or non-NASH+L-NAME groups. The SHRSP5/Dmcr rat model demonstrates that NASH significantly aggravates cardiovascular risk.

DOI： 10.1111/iep.12301

SHRSP5/Dmcr rat fed with high-fat and high-cholesterol diet induces cardiac fibrosis, vascular endothelial and left ventricular diastolic dysfunction 査読

Shogo Watanabe, Shota Kumazaki, Katsuhiro Kusunoki, Terumi Inoue, Yui Maeda, Shinichi Usui, Ryoko Shinohata, Takashi Ohtsuki, Satoshi Hirohata, Shozo Kusachi, Kazuya Kitamori, Mari Mori, Yukio Yamori, and Hisao Oka

Journal of Atherosclerosis and Thrombosis 25 ( 5 ) 439 - 453 2018年

Novel Mechanomyogram/electromyogram Hybrid Transducer Measurements Reflect Muscle Strength during Dynamic Exercise ̶ Pedaling of Recumbent Bicycle ̶ 査読

Shinichi Fukuhara, Shogo Watanabe, Hisao Oka

Advanced Biomedical Engineering 7 47 - 54 2018年

SHRSP5/Dmcrラットにおける循環器系の基礎病態解析査読

渡辺彰吾, 熊崎章太

SHR等疾患モデル共同研究会News letter 2018年

Estimating the minimum stimulation frequency necessary to evoke tetanic progression based on muscle twitch parameters 査読

Shogo Watanabe, Shinichi Fukuhara, Takeshi Fujinaga and Hisao Oka

Physiological Measurement 38 ( 3 ) 466 - 476 2017年3月

The developed MMG / EMG Hybrid Transducer reflects Muscle Strength during Dynamic Exercise - Pedaling of Recumbent Bicycle - 査読

Shinichi Fukuhara, Shogo Watanabe, Hisao Oka

Advanced Biomedical Engineering 7 47 - 54 2017年

5チャンネル同時計測型MMG/EMGハイブリッドセンサシステムの開発査読

福原真一, 渡辺彰吾, 岡久雄

生体医工学 55 ( 4 ) 252 - 252 2017年

記述言語：日本語出版者・発行元：公益社団法人日本生体医工学会

筋音図（Mechanomyoram : MMG）と筋電図（Electromyogram : EMG）を同時計測することにより、骨格筋収縮の正確な評価が可能となる。しかし、筋音図は身体を固定して計測しなければならない等の理由からこれまで用いられることが少なかった。そこで筆者らは、筋音図と筋電図の同時計測が可能なMMG/EMGハイブリッドセンサシステムを開発した。デバイス本体は小型軽量（47×34×24mm、34g）であり、簡便に皮膚に貼付できる。ハイブリッドセンサは、筋音／筋電センサ部、トランスデューサ部およびストレージ（SDカード）が一体となっていることから、付属装置を必要とせずに筋音／筋電計測を行うことができる。従来、センサの固定に微妙な調節が必要であったが、センサ形状に合わせた専用のベルトを用いることにより、簡単に取り付けが可能となった。また、センサと操作端末はBluetooth(R)を介して通信され、計測・制御ソフトウェアによって最大5台のハイブリッドセンサを一括制御でき、サンプリング時間や測定時間等の計測パラメータが設定できる。筋音／筋電波形はリアルタイムモニタリングされ，データはCSV形式で出力される。MMG/EMGハイブリッドセンサシステムの開発により、適用フィールドを問わず、誰もが容易に筋音／筋電計測を行えるようになった。謝辞本研究の一部は、H28年度特別電源所在県科学技術振興事業（岡山県）の助成を受けた。記して謝意を表する。

DOI： 10.11239/jsmbe.55Annual.252

Electrically induced mechanomyograms reflect inspiratory muscle strength in young or elderly subjects 査読

Shogo Watanabe, Ippei Nojima, Yuuna Agarie, Tatsunori Watanabe, Shinichi Fukuhara, Takeshi Fujinaga, Hisao Oka

Respiratory Investigation 54 ( 6 ) 436 - 444 2016年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Elsevier B.V.

Background Respiratory muscle strength has been used as a tool for evaluating respiratory rehabilitation in chronic obstructive pulmonary disease. However, mouth pressure measurement evaluated by maximum expiratory mouth pressure (PEmax) or inspiratory mouth pressure (PImax) offers an indirect method for measuring respiratory muscle strength. We demonstrated the evaluation of diaphragm contractility using a mechanomyogram (MMG), which is the mechanical signal generated by the motion of the diaphragm induced by the electric stimulation of the phrenic nerve. Methods Study participants were 21 young and 20 elderly subjects with no symptoms of respiratory disease. The elderly subjects were divided into non-smoker or smoker groups. The smoker group was defined as subjects having a Brinkman Index of greater than 300. We measured basic spirometric parameters, mouth pressure (PEmax, PImax), and diaphragmatic MMG. Results Diaphragmatic MMG showed more clear contrast between young subjects and elderly non-smoker or smoker subjects than the conventional method for respiratory muscle contraction (PEmax, PImax). In addition, the diaphragmatic MMG strongly correlated with inspiratory muscle strength. Conclusions Diaphragmatic MMG may reflect diaphragmatic contractility more directly and sensitively than the conventional method.

DOI： 10.1016/j.resinv.2016.06.001

Scopus

Enhancement of arterial pulsation during flow-mediated dilation is impaired in the presence of ischemic heart disease 査読

Eisuke Amiya,Masafumi Watanabe,Shogo Watanabe*,Munenori Takata,Issei Komuro

SpringerPlus 5 ( 1 ) 2016年7月

Enhancement of arterial pulsation during flow-mediated dilation is impaired in the presence of ischemic heart disease 査読

Eisuke Amiya,Masafumi Watanabe,Shogo Watanabe*,Munenori Takata,Issei Komuro

SpringerPlus 5 ( 1103 ) 2016年7月

横隔膜筋音図と呼吸筋力の関係査読

渡辺彰吾, 福原真一, 藤長武士, 岡久雄

電子情報通信学会技術研究報告 116 ( 170 ) 25 - 28 2016年7月

Evaluation of skeletal muscle contraction during pedaling of recumbent bicycle using MMG/EMG hybrid transducer 査読

Shinichi Fukuhara, Takeshi Fujinaga, Shogo Watanabe, Hisao Oka

BME = Bio medical engineering / henshu, Nihon ME Gakkai, Japanese Journal of Medical Electronics and Biological Engineering, Transactions of Japanese Society for Medical and Biological Engineering, BME = Bio medical engineering / henshu, Nihon ME Gakkai 54 ( PROC ) 1 - 2 2016年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：一般社団法人日本生体医工学会

Electromyogram (EMG) is recorded electrical muscle contraction, mechanomyogram (MMG) indicates cross-sectional area change of muscle, reflect mechanical muscle contraction. By simultaneously measuring both signals, it is possible to multifaceted evaluation of muscle contraction. However, MMG measurement at voluntary movement was difficult. Therefore, the authors developed MMG / EMG hybrid transducer capable of simultaneous measurement of MMG and EMG. This study evaluated MMG and EMG of rectus femoris (RF) and hamstrings using recumbent bicycle of easy load regulation. As result, dMMGbase (baseline of displacement MMG) indicated cross-sectional area change of muscle at pushing down and pulling up of pedal. In addition, EMG and dMMGbase increased with pushing down of pedal in RF, dMMGacc (acceleration dMMGbase) showing muscle contraction force was output simultaneously. Each signal of hamstrings had output that antagonize RF. By simultaneously measuring MMG and EMG at recumbent bicycle pedaling, it was possible to evaluate muscle contraction of voluntary movement.

DOI： 10.11239/jsmbe.54Annual.P2-C04-1

Blockade of glucocorticoid receptors with RU486 attenuates cardiac damage and adipose tissue inflammation in a rat model of metabolic syndrome 査読

Takeshita Y, Watanabe S, Hattori T, Nagasawa K, Matsuura N, Takahashi K, Murohara T, Nagata K

Hypertens Res 38 ( 11 ) 741 - 750 2015年11月

Restraint stress exacerbates cardiac and adipose tissue pathology via β-adrenergic signaling in rats with metabolic syndrome 査読

Matsuura N, Nagasawa K, Minagawa Y, Ito S, Sano Y, Yamada Y, Hattori T, Watanabe S, Murohara T, Nagata K

American Journal of Physiology-Heart and Circulatory Physiology 308 ( 10 ) H1275 - H1286 2015年5月

Roles of oxidative stress and the mineralocorticoid receptor in cardiac pathology in a rat model of metabolic syndrome 査読

Takahashi K, Murase T, Takatsu M, Matsuura N, Nagasawa K, Hattori T, Watanabe S, Murohara T, Nagata K

Nagoya Journal of Medical Science 77 ( 1-2 ) 275 - 289 2015年2月

Comparative effects of valsartan in combination with cilnidipine or amlodipine on cardiac remodeling and diastolic dysfunction in Dahl salt-sensitive rats 査読

Kai Nagasawa, Keiji Takahashi, Natsumi Matsuura, Miwa Takatsu, Takuya Hattori, Shogo Watanabe, Eri Harada, Kazumi Niinuma, Toyoaki Murohara, Kohzo Nagata

HYPERTENSION RESEARCH 38 ( 1 ) 39 - 47 2015年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：NATURE PUBLISHING GROUP

Angiotensin receptor blockers (ARBs) are often supplemented with calcium channel blockers (CCBs) for treatment of hypertension. We recently showed that the L/N-type CCB cilnidipine has superior cardioprotective effects compared with the L-type CCB amlodipine in Dahl salt-sensitive (DS) rats. We have now compared the effects of the ARB valsartan combined with cilnidipine or amlodipine on cardiac pathophysiology in DS rats. DS rats fed a high-salt diet from 6 weeks of age were treated with vehicle, valsartan alone (10 mg kg(-1) per day), or valsartan combined with either cilnidipine (1 mg kg(-1) per day) or amlodipine (1 mg kg(-1) per day) from 7 to 11 weeks. The salt-induced increase in systolic blood pressure apparent in the vehicle group was attenuated similarly in the three drug treatment groups. Valsartan-cilnidipine attenuated left ventricular (LV) fibrosis and diastolic dysfunction as well as cardiac oxidative stress and inflammation to a greater extent than did valsartan alone or valsartan-amlodipine. In addition, the increases in urinary excretion of dopamine and epinephrine as well as in cardiac renin-angiotensin-aldosterone-system (RAAS) gene expression apparent in vehicle-treated rats were attenuated to a greater extent by valsartan-cilnidipine than by the other two treatments. Valsartan-cilnidipine thus attenuated LV remodeling and diastolic dysfunction more effectively than did valsartan or valsartan-amlodipine in rats with salt-sensitive hypertension, and this superior cardioprotective action of valsartan-cilnidipine compared with valsartan-amlodipine is likely attributable, at least in part, to the greater antioxidant and antiinflammatory effects associated with both greater inhibition of cardiac RAAS gene expression and N-type calcium channel blockade.

DOI： 10.1038/hr.2014.136

Web of Science

New mathematical model of isometric twitch force-response curve by skeletal muscle contraction; using extended hybrid logistic model 査読

Effects of pioglitazone on cardiac and adipose tissue pathology in rats with metabolic syndrome 査読

Matsuura N, Asano C, Nagasawa K, Ito S, Sano Y, Minagawa Y, Yamada Y, Hattori T, Watanabe S, Murohara T, Nagata K

International Journal of Cardiology 179 ( 20 ) 360 - 369 2015年1月

Dietary salt restriction improves cardiac and adipose tissue pathology independently of obesity in a rat model of metabolic syndrome. 査読

Hattori T, Murase T, Takatsu M, Nagasawa K, Matsuura N, Watanabe S, Murohara T, Nagata K.

J Am Heart Assoc. 3 ( 6 ) 2014年12月

Elevated C-reactive protein levels and enhanced high frequency vasomotion in patients with ischemic heart disease during brachial flow-mediated dilation 査読

Shogo Watanabe, Eisuke Amiya, Masafumi Watanabe, Munenori Takata, Atsuko Ozeki, Aya Watanabe, Shuichi Kawarasaki, Tomoko Nakao, Yumiko Hosoya, Kohzo Nagata, Ryozo Nagai, Issei Komuro

PLoS One 9 ( 10 ) 2014年10月

Effect of Add-on Aliskiren to Type 1 Angiotensin Receptor Blocker Therapy on Endothelial Function and Autonomic Nervous System in Hypertensive Patients With Ischemic Heart Disease 査読国際誌

Journal of Clinical Hypertension 16 ( 8 ) 591 - 598 2014年8月

Generation of rat-induced pluripotent stem cells from a new model of metabolic syndrome 査読

Nana Takenaka-Ninagawa, Yuka Kawabata, Shogo Watanabe, Kohzo Nagata, Shigeko Torihashi

PLoS One 9 ( 8 ) 2014年8月

Diurnal body temperature rise is reduced in diabetes with autonomic neuropathy 査読国際誌

Clinical Autonomic Research 24 ( 2 ) 95 - 7 2014年4月

Premature cardiac senescence in DahlS.Z-Leprfa/Leprfa rats as a new animal model of metabolic syndrome 査読

Takahashi K, Takatsu M, Hattori T, Murase T, Ohura S, Takeshita Y, Watanabe S, Murohara T, Nagata K

Nagoya Journal of Medical Science 76 ( 1-2 ) 35 - 49 2014年2月

Glucocorticoids activate cardiac mineralocorticoid receptors in adrenalectomized Dahl salt-sensitive rats 査読

Ohtake M, Hattori T, Murase T, Takahashi K, Takatsu M, Ohtake M, Miyachi M, Watanabe S, Cheng XW, Murohara T, Nagata K

Nagoya Journal of Medical Science 76 ( 1-2 ) 59 - 72 2014年2月

Differences in Body Temperature Variability Between Subjects With and Without Diabetes and Predictive Value for Cardiovascular Events 査読

Eisuke Amiya, Masafumi Watanabe, Munenori Takata, Shogo Watanabe, Atsuko Ozeki, Aya Watanabe, Shuichi Kawarasaki, Tomoko Nakao, Yumiko Hosoya, Kazuko Omori, Koji Maemura, Yasunobu Hirata, Ryozo Nagai, Issei Komuro

Circulation Journal 77 ( 7 ) 1844 - 1853 2013年7月

Simultaneous heart rate variability monitoring enhances the predictive value of flow-mediated dilation in ischemic heart disease 査読

Watanabe, Shogo, Amiya, Eisuke, Watanabe, Masafumi, Takata, Munenori, Ozeki, Atusko, Watanabe, Aya, Kawarasaki, Shuichi, Hosoya, Yumiko, Nakao, Tomoko, Ohmori, Kazuko, Maemura, Koji, Komuro, Issei, Nagai, Ryozo

Circulation Journal 77 ( 4 ) 1018 - 1025 2013年

The relationship between Fusion index ? Frequency Curve of Displacement ? MMG and Fiber Composition of Skeletal Muscle 査読

渡辺彰吾, 北脇知己, 岡久雄

バイオメカニズム 20 2017 - 216 2011年

Mathemaical equation of fusion index of tetanic contraction of skeletal muscles 査読

Shogo WATANABE, Tomoki KITAWAKI, Hisao OKA

Journal of electromyography and kinesiology 2010年

変位筋音図のFusion index曲線と筋線維構成比との関係査読

渡辺彰吾, 北脇知己, 岡久雄

バイオメカニズム２０ 20 207 - 216 2010年

北脇知己, 渡辺彰吾, 岡久雄

生体医工学 47 ( 2 ) 199 - 208 2009年

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：一般社団法人日本生体医工学会

Isometric force response curve by electrically stimulated skeletal muscle contraction of twitch is important in order to judge the ability of the skeletal muscle and to distinguish disease state. In this report, we introduce a new mathematical model of the isometric force response curve, in order to estimate the muscle contraction behavior accurately. The new mathematical model of force response curve was expressed by a difference of fundamental function which was expressed by products of a step response function and a logistic function. The mathematical model was applied to the force response curve by electrically stimulated muscle contraction of twitch, using the rat gastrocnemius muscle (GC), intermediate vastus muscle (VI) and soleus muscle (SOL) . The results indicate new mathematical model showed better agreement with the force response curve of twitch than previous mathematical model in the rat skeletal muscle which has different muscle fiber type only changes the model parameters.

DOI： 10.11239/jsmbe.47.199

CiNii Books

その他リンク： http://search.jamas.or.jp/link/ui/2009345502

Estimation of skeletal muscle fatigue by a displacement MMG : in the case of isometric elbow flexion at 80% MVC 査読

石井圭, 渡辺彰吾, 岡久雄

電子情報通信学会技術研究報告(MEとバイオサイバネティックス 108 ( 479 ) 2009年

Consideration of Tetanic Contraction Properties of Different Skeletal Muscle Fibers Using Displacement MMG 査読

渡辺彰吾, 栗山雄樹, 北脇知己, 岡久雄

バイオメカニズム 19 23 - 33 2008年

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：バイオメカニズム学会

骨格筋収縮に起因する微細な振動を記録した筋音図MMG (mechanomyogram) は, 筋の機械的な収縮機能を反映しているが, 強縮時における筋の断面積方向の変位を測定した報告は少ない. 著者らはヒト大腿直筋 (type Ⅱ線維優位) および短母指外転筋 (type Ⅰ線維優位) の皮膚表面において, 30秒間に0.2 [Hz] から50 [Hz] まで連続的に電気刺激の周波数を増加させたときのMMGを, レーザ変位計によって測定し, 骨格筋の強縮特性について検討した. 実験は筋疾患のない21～25歳の男性3名, 女性1名で行った. 本実験より, (1) 異なる筋線維タイプが混在する骨格筋は, 二段階的に不完全強縮が進行し, 筋線維の構成比によって変位MMGの大きさが異なる, (2) 一般的なtype Ⅰ, type Ⅱ線維の単収縮 (筋張力) の収縮時間と弛緩時間から, 強縮過程における変曲点の周波数域を推定した結果, 最初に起こる不完全強縮はtype Ⅰ線維, 続いて起こる不完全強縮はtype Ⅱ線維の周波数域とほぼ一致する, ことがわかった.

DOI： 10.3951/biomechanisms.19.23

Development of displacement MMG sensor using a photo-reflector 査読

岡久雄, 栗山雄樹, 渡辺彰吾, 北脇知己, 岡本基

電子情報通信学会技術研究報告(MEとサイバネティックス) 107 ( 541 ) 2008年

Evaluation of muscle fatigue with the different muscle fiber type during running exercise using MMG 査読

栗山雄樹, 渡辺彰吾, 北脇知己, 岡久雄

電子情報通信学会技術研究報告(MEとサイバネティックス) 106 ( 592 ) 2007年

Consideration of Running Form Using Lower Leg Electrical Impedance 査読

中村隆夫, 北脇知己, 渡辺彰吾, 楠原俊昌, 矢野博己, 池田敏, 加藤浩, 山本尚武, 岡久雄

電子情報通信学会技術研究報告(MEとサイバネティックス) 107 ( 154 ) 2007年

Muscle contraction accelerates IL-6 mRNA expression in the rat masseter muscle 査読国際誌

Ono T, Maekawa K, Watanabe S, Oka H, Kuboki T

Archives of Oral Biology 52 ( 5 ) 479 - 86 2007年

筋内圧力および生体表面の変位に着目した筋音図発生メカニズムの検討査読

渡辺彰吾, 北脇知己, 岡久雄

バイオメカニズム 18 209 - 218 2006年

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：バイオメカニズム学会

筋音図 (MMG) は, 筋の電気的な活動を記録する筋電図とは異なり, 筋の機械的な機能を反映していると言われるが, その発生メカニズムは十分に解明されていない. 本研究ではラットの体表上に加速度センサ, レーザ変位計を設置し, 同時に光ファイバ型圧力センサを筋内に挿入して, 腓腹筋を電気刺激したときの, 加速度MMG, 表面変位, 筋内圧力を測定した. そして, 単収縮を誘発した際の加速度MMG, 表面変位, 筋内圧力の潜時, 伝播速度等の比較や, 筋小胞体からのCa^2＋放出を抑制するdantroleneを筋注したときの各波形の変化を測定し, MMGの発生メカニズムについて考察した. 本研究から, (1) 加速度センサを用いて測定されたMMGは力学的に変位に変換が可能である, (2) 加速度MMG は筋収縮に伴う筋内圧力の変化によって生じる側方へのせん断 (shear) 波の伝播, 合成によるものである, (3) 加速度MMG の潜時差より, 神経・筋接合部の所在を推定することが可能である, 等の知見が得られた.

DOI： 10.3951/biomechanisms.18.209