2021/10/21 更新

写真a

バンドウ テツヤ
板東 哲哉
BANDO Tetsuya
所属
医歯薬学域 講師
職名
講師
外部リンク

学位

  • 博士(理学) ( 岡山大学 )

研究キーワード

  • 器官再生

  • Regeneration

研究分野

  • ライフサイエンス / ゲノム生物学

  • ライフサイエンス / 発生生物学

  • ライフサイエンス / システムゲノム科学

学歴

  • 岡山大学   Graduate School, Division of Science and Technology  

    - 2005年

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  • 岡山大学    

    - 2005年

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    国名: 日本国

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  • 岡山大学   Graduate School, Division of Natural Science  

    - 1999年

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  • 岡山大学    

    - 1999年

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    国名: 日本国

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  • 岡山大学   理学部   生物学科

    - 1997年

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    国名: 日本国

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  • 岡山大学   Faculty of Science  

    - 1997年

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経歴

  • - 岡山大学医歯薬学総合研究科 講師

    2016年

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  • - Senior Assistant Professor,Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,Okayama University

    2016年

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  • Assistant Professor,Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,Okayama University

    2012年 - 2016年

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  • 岡山大学医歯薬学総合研究科 助教

    2012年 - 2016年

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  • Researcher

    2009年 - 2012年

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  • 徳島大学

    2009年 - 2012年

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  • Researcher

    2008年 - 2009年

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  • 科学技術振興機構 研究員

    2008年 - 2009年

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  • Researcher

    2005年 - 2008年

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  • 徳島大学

    2005年 - 2008年

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所属学協会

 

論文

  • Dkk3/REIC, an N-glycosylated Protein, Is a Physiological Endoplasmic Reticulum Stress Inducer in the Mouse Adrenal Gland.

    Hirofumi Fujita, Tetsuya Bando, Seiichi Oyadomari, Kazuhiko Ochiai, Masami Watanabe, Hiromi Kumon, Hideyo Ohuchi

    Acta medica Okayama   74 ( 3 )   199 - 208   2020年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Dickkopf 3 (Dkk3) is a secreted protein belonging to the Dkk family and encoded by the orthologous gene of REIC. Dkk3/REIC is expressed by mouse and human adrenal glands, but the understanding of its roles in this organ is still limited. To determine the functions of Dkk3 in the mouse adrenal gland, we first identified that the mouse Dkk3 protein is N-glycosylated in the adrenal gland as well as in the brain. We performed proteome analysis on adrenal glands from Dkk3-null mice, in which exons 5 and 6 of the Dkk3 gene are deleted. Twodimensional polyacrylamide gel electrophoresis of adrenal proteins from wild-type and Dkk3-null mice revealed 5 protein spots whose intensities were altered between the 2 genotypes. Mass spectrometry analysis of these spots identified binding immunoglobulin protein (BiP), an endoplasmic reticulum (ER) chaperone. To determine whether mouse Dkk3 is involved in the unfolded protein response (UPR), we carried out a reporter assay using ER-stress responsive elements. Forced expression of Dkk3 resulted in the induction of distinct levels of reporter expression, showing the UPR initiated by the ER membrane proteins of activating transcription factor 6 (ATF6) and inositol-requring enzyme 1 (IRE1). Thus, it is possible that Dkk3 is a physiological ER stressor in the mouse adrenal gland.

    DOI: 10.18926/AMO/59950

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  • Dickkopf3 (Dkk3) is required for maintaining the integrity of secretory vesicles in the mouse adrenal medulla. 査読

    Habuta M, Fujita H, Sato K, Bando T, Inoue J, Kondo Y, Miyaishi S, Kumon H, Ohuchi H

    Cell and tissue research   2019年10月

  • Localization of the ultraviolet-sensor Opn5m and its effect on myopia-related gene expression in the late-embryonic chick eye. 査読

    Kato M, Sato K, Habuta M, Fujita H, Bando T, Morizane Y, Shiraga F, Miyaishi S, Ohuchi H

    Biochemistry and biophysics reports   19   100665   2019年9月

  • Bone morphogenetic protein signaling in distal patterning and intercalation during leg regeneration of the cricket, Gryllus bimaculatus. 査読

    Ishimaru Y, Bando T, Ohuchi H, Noji S, Mito T

    Development, growth & differentiation   60 ( 6 )   377 - 386   2018年8月

  • Leg formation and regeneration 査読

    Tetsuya Bando, Yoshimasa Hamada, Sumihare Noji

    The Cricket as a Model Organism: Development, Regeneration, and Behavior   31 - 48   2017年1月

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    掲載種別:論文集(書籍)内論文  

    © Springer Japan KK 2017. All rights reserved. In contrast to higher vertebrates, orthopteran nymphs have remarkable regenerative capacity for regrowing complex morphological structures and organs. In this review, we summarize the molecular basis of tissue regeneration in the cricket Gryllus bimaculatus. In this species, the lost part of a leg can be regenerated epimorphically from blastema cells, a population of dedifferentiated proliferating cells. Blastema cell proliferation is regulated by JAK/STAT and Salvador/Warts/Hippo signaling pathways. The positional information for leg regrowth, which includes the recognition of amputated position and proper regeneration, is maintained by Dachsous/Fat signaling. The regrowth of lost leg segments is reconstructed through the expressions of genes in the hedgehog, wingless, decapentaplegic, and Egf signaling pathways and epigenetic modifiers E(z) and Utx. The insights obtained reveal the high level of conservation between insects and vertebrates, suggesting that Gryllus may be a suitable model for human regenerative medicine studies.

    DOI: 10.1007/978-4-431-56478-2_3

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  • Eye development and photoreception of a hemimetabolous insect, gryllus bimaculatus 査読

    Hideyo Ohuchi, Tetsuya Bando, Taro Mito, Sumihare Noji

    The Cricket as a Model Organism: Development, Regeneration, and Behavior   49 - 62   2017年1月

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    記述言語:英語   掲載種別:論文集(書籍)内論文   出版者・発行元:Springer Japan  

    The hemimetabolous insect, Gryllus bimaculatus, has two compound eyes that begin to form in the embryo and increase in size five- to sixfolds during nymphal development. Retinal stemlike cells reside in the anteroventral proliferation zone (AVPZ) of the nymphal compound eye and proliferate to increase retinal progenitors, which then differentiate to form new ommatidia in the anterior region of the eye. Here, we introduce the morphology and development of the cricket eye first, and then we focus on the roles of retinal determination genes (RDGs) such as eyes absent (eya) and sine oculis (so) in Gryllus eye formation and growth. Since the principal function of the eye is photoreception, we finally summarize opsin photopigments in this species, broadening the roles of photoreception.

    DOI: 10.1007/978-4-431-56478-2_4

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  • Cricket body size is altered by systemic RNAi against insulin signaling components and epidermal growth factor receptor 査読

    Noha Dabour, Tetsuya Bando, Taro Nakamura, Katsuyuki Miyawaki, Taro Mito, Hideyo Ohuchi, Sumihare Noji

    DEVELOPMENT GROWTH & DIFFERENTIATION   53 ( 7 )   857 - 869   2011年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    A long-standing problem of developmental biology is how body size is determined. In Drosophila melanogaster, the insulin/insulin-like growth factor (I/IGF) and target of rapamycin (TOR) signaling pathways play important roles in this process. However, the detailed mechanisms by which insect body growth is regulated are not known. Therefore, we have attempted to utilize systemic nymphal RNA interference (nyRNAi) to knockdown expression of insulin signaling components including Insulin receptor (InR), Insulin receptor substrate (chico), Phosphatase and tensin homologue (Pten), Target of rapamycin (Tor), RPS6-p70-protein kinase (S6k), Forkhead box O (FoxO) and Epidermal growth factor receptor (Egfr) and observed the effects on body size in the Gryllus bimaculatus cricket. We found that crickets treated with double-stranded RNA (dsRNA) against Gryllus InR, chico, Tor, S6k and Egfr displayed smaller body sizes, while Gryllus FoxO nyRNAi-ed crickets exhibited larger than normal body sizes. Furthermore, RNAi against Gryllus chico and Tor displayed slow growth and RNAi against Gryllus chico displayed longer lifespan than control crickets. Since no significant difference in ability of food uptake was observed between the Gryllus chico(nyRNAi) nymphs and controls, we conclude that the adult cricket body size can be altered by knockdown of expressions of Gryllus InR, chico, Tor, S6k, FoxO and Egfr by systemic RNAi. Our results suggest that the cricket is a promising model to study mechanisms underlying controls of body size and life span with RNAi methods.

    DOI: 10.1111/j.1440-169X.2011.01291.x

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  • Lowfat, a Mammalian Lix1 Homologue, Regulates Leg Size and Growth Under the Dachsous/Fat Signaling Pathway During Tissue Regeneration 査読

    Tetsuya Bando, Yoshimasa Hamada, Kazuki Kurita, Taro Nakamura, Taro Mito, Hideyo Ohuchi, Sumihare Noji

    DEVELOPMENTAL DYNAMICS   240 ( 6 )   1440 - 1453   2011年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    In the cricket Gryllus bimaculatus, missing distal parts of amputated legs are regenerated from blastemas based on positional information. The Dachsous/Fat (Ds/Ft) signaling pathway regulates blastema cell proliferation and positional information along the longitudinal axis during leg regeneration. Herein, we show that the Gryllus homologue of Lowfat (Gb'Lft), which modulates Ds/Ft signaling in Drosophila, is involved in leg regeneration. Gb'lft is expressed in regenerating legs, and RNAi against Gb'lft (Gb'lft RNAi) suppressed blastema cell hyperproliferation caused by Gb'ft(RNAi) or Gb'ds(RNAi) but enhanced that caused by Gb'kibra(RNAi) or Gb'warts(RNAi). In Gb'lft RNAi nymphs, missing parts of amputated legs were regenerated, but the length of the regenerated legs was shortened depending on the position of the amputation. Both normal and reversed intercalary regeneration occurred in Gb'lft(RNAi) nymphs, suggesting that Gb'Lft is involved in blastema cell proliferation and longitudinal leg regeneration under the Ds/Ft signaling pathway, but it is not required for intercalary regeneration. Developmental Dynamics 240:1440-1453, 2011. (C) 2011 Wiley-Liss, Inc.

    DOI: 10.1002/dvdy.22647

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  • Regulation of Leg Size and Shape: Involvement of the Dachsous-Fat Signaling Pathway 査読

    Tetsuya Bando, Taro Mito, Taro Nakamura, Hideyo Ohuchi, Sumihare Noji

    DEVELOPMENTAL DYNAMICS   240 ( 5 )   1028 - 1041   2011年5月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    How limb size and shape is regulated is a long-standing question in developmental and regeneration biology. Recently, the protocadherin Dachsous-Fat (Ds-Ft) signaling pathway has been found to be essential for wing development of the fly and leg regeneration of the cricket. The Ds-Ft signaling pathway is linked to the Warts-Hippo (Wts-Hpo) signaling pathway, leading to cell proliferation. Several lines of evidence have suggested that the Wts-Hpo signaling pathway is involved in the control of organ size, and that this pathway is regulated by Ds-Ft and Merlin-Expanded, which are linked to morphogens such as decapentaplegic/bone morphogenic protein, Wingless/Wnt, and epidermal growth factor. Here we review recent progress in elucidating mechanisms controlling leg size and shape in insects and vertebrates, focusing on the Ds-Ft signaling pathway. We also introduce a working model, Ds-Ft steepness model, to explain how steepness of the Ds-Ft gradient controls leg size along the proximodistal axis. Developmental Dynamics 240:1028-1041, 2011. (C) 2011 Wiley-Liss, Inc.

    DOI: 10.1002/dvdy.22590

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  • Imaging of Transgenic Cricket Embryos Reveals Cell Movements Consistent with a Syncytial Patterning Mechanism 査読

    Taro Nakamura, Masato Yoshizaki, Shotaro Ogawa, Haruko Okamoto, Yohei Shinmyo, Tetsuya Bando, Hideyo Ohuchi, Sumihare Noji, Taro Mito

    CURRENT BIOLOGY   20 ( 18 )   1641 - 1647   2010年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CELL PRESS  

    The mode of insect embryogenesis varies among species, reflecting adaptations to different lite history strategies [1, 2]. In holometabolous insects, which include the model systems, such as the fruit fly and the red flour beetle, a large proportion of the blastoderm produces an embryo, whereas hemimetabolous embryos generally arise from a small region of the blastoderm [3]. Despite their importance in evolutionary studies, information of early developmental dynamics of hemimetabolous insects remains limited. Here, to clarify how maternal and gap gene products act in patterning the embryo of basal hemimetabolous insects, we analyzed the dynamic segmentation process in transgenic embryos of an intermediate-germ insect species, the cricket, Gryllus bimaculatus. Our data based on live imaging of fluorescently labeled embryonic cells and nuclei suggest that the positional specification of the cellular blastoderm may be established in the syncytiurn, where maternally derived gradients could act fundamentally in a way that is similar to that of Drosophila, namely throughout the egg. Then, the blastoderm cells move dynamically, retaining their positional information to form the posteriorly localized germ anlage. Furthermore, we find that the anterior head region of the cricket embryo is specified by orthodenticle in a cellular environment earlier than the gnathal and thoracic regions. Our findings imply that the syncytial mode of the early segmentation in long-germ insects evolved from a dynamic syncytial-to-cellular mode found in the present study, accompanied by a heterochronic shift of gap gene action.

    DOI: 10.1016/j.cub.2010.07.044

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  • Regulation of leg size and shape by the Dachsous/Fat signalling pathway during regeneration 査読

    Tetsuya Bando, Taro Mito, Yuko Maeda, Taro Nakamura, Fumiaki Ito, Takahito Watanabe, Hideyo Ohuchi, Sumihare Noji

    DEVELOPMENT   136 ( 13 )   2235 - 2245   2009年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:COMPANY OF BIOLOGISTS LTD  

    An amputated cricket leg regenerates all missing parts with normal size and shape, indicating that regenerating blastemal cells are aware of both their position and the normal size of the leg. However, the molecular mechanisms regulating this process remain elusive. Here, we use a cricket model to show that the Dachsous/Fat (Ds/Ft) signalling pathway is essential for leg regeneration. We found that knockdown of ft or ds transcripts by regeneration-dependent RNA interference (rdRNAi) suppressed proliferation of the regenerating cells along the proximodistal (PD) axis concomitantly with remodelling of the pre-existing stump, making the regenerated legs shorter than normal. By contrast, knockdown of the expanded (ex) or Merlin (Mer) transcripts induced over-proliferation of the regenerating cells, making the regenerated legs longer. These results are consistent with those obtained using rdRNAi during intercalary regeneration induced by leg transplantation. We present a model to explain our results in which the steepness of the Ds/Ft gradient controls growth along the PD axis of the regenerating leg.

    DOI: 10.1242/dev.035204

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  • Tissue-specific interactions of TNI isoforms with other TN subunits and tropomyosins in C. elegans: the role of the C- and N-terminal extensions. 査読

    Amin MZ, Bando T, Ruksana R, Anokye-Danso F, Takashima Y, Sakube Y, Kagawa H

    Biochim Biophys Acta   1774   456 - 465   2007年4月

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    出版者・発行元:4  

    DOI: 10.1016/j.bbapap.2007.01.003

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  • A novel non-coding DNA family in Caenorhabditis elegans 査読

    Yasuo Takashima, Tetsuya Bando, Hiroaki Kagawa

    GENE   388 ( 1-2 )   61 - 73   2007年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Many repetitive elements, for example, SINEs, LINEs, LTR-retrotransposons and other SSRs are dispersed throughout eukaryotic genomes. To understand the biological function of these repetitive elements is of great current research interest. In this study, we report on the identification of a novel non-coding DNA family, designated CE1 family, in the nematode C. elegans genome. Some CE1 elements constituted a large palindrome sequence. The CE1 elements were interspersed at 95 sites in the C. elegans genome. Most of the CE1 elements were associated with, or were within, protein-coding genes. The sequence of the CE1 elements indicated that some could form a hairpin structure. One of the CE1 family, CE1 (bs258), is located in the first intron of a novel gene, C46H11.6 which encodes a PDZ/DHR/GLGF domain protein. In gfp and lacZ reporter gene assays the CE1 (bs258) element appeared to behave as an enhancer element for the expression of C46H11.6 but no effect on the expression of the opposite direction gene, pat-10 which encodes the body-wall muscle troponin C. The CE1 (bs258) RNA transcript was detected by RT-PCR even when CE1(bs258) was located in an intron. We conclude that CE1 elements are involved in the expression of adjacent genes and are therefore selectively retained in the C. elegans genome. We discussed a biological function of the CE1(bs258) having many transcription factor-binding sites. (c) 2006 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.gene.2006.10.002

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  • Involvement of canonical Wnt/Wingless signaling in the determination of the positional values within the leg segment of the cricket Gryllus bimaculatus 査読

    Taro Nakamura, Taro Mito, Yoshihisa Tanaka, Tetsuya Bando, Hideyo Ohuchi, Sumihare Noji

    DEVELOPMENT GROWTH & DIFFERENTIATION   49 ( 2 )   79 - 88   2007年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING  

    The cricket Gryllus bimaculatus is a hemimetabolous insect whose nymphs posses the ability to regenerate amputated legs. Previously, we showed that Gryllus orthologues of Drosophila hedgehog (Gb'hh), wingless (Gb'wg) and decapentaplegic (Gb'dpp) are expressed during leg regeneration and play essential roles in the establishment of the proximal-distal axis. Here, we examined their roles during intercalary regeneration: when a distally amputated tibia with disparate positional values is placed next to a proximally amputated host, intercalary growth occurs in order to regenerate the missing part. In this process, we examined expression patterns of Gb'hh and Gb'wg. We found that expressions of Gb'hh and Gb'wg were induced in a regenerate and the host proximal to the amputated region, but not in the grafted donor distal to the regenerate. This directional induction occurs even in the reversed intercalation. Because these results are consistent with a distal-to-proximal respecification of the regenerate, Gb'wg may be involved in the re-establishment of the positional values in the regenerate. Furthermore, we found that no regeneration occurs when Gb'armadillo (the orthologue of beta-catenin) was knocked down by RNA interference. These results indicate that the canonical Wnt/Wingless signaling pathway is involved in the process of leg regeneration and determination of positional information in the leg segment.

    DOI: 10.1111/j.1440-169x.2007.00915.x

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  • Tissue expression of four troponin I genes and their molecular interactions with two troponin C isoforms in Caenorhabditis elegans. 査読

    Ruksana R, Kuroda K, Terami H, Bando T, Kitaoka S, Takaya T, Sakube Y, Kagawa H

    Genes Cells   10 ( 3 )   261-276   2005年

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MISC

  • Congenital eye anomalies: More mosaic than thought? 査読

    Ohuchi H, Sato K, Habuta M, Fujita H, Bando T

    Congenital anomalies   2018年7月

  • timeless2 plays an important role in reproduction and circadian rhythms in the cricket Gryllus bimaculatus

    Motoki Nose, Atsushi Tokuoka, Tetsuya Bando, Kenji Tomioka

    Journal of Insect Physiology   105   9 - 17   2018年2月

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    記述言語:英語   出版者・発行元:Elsevier Ltd  

    The timeless2 (tim2) gene is an insect orthologue of the mammalian clock gene Timeless (mTim). Although its functional role has been extensively studied in mammals, little is known regarding its role in insects. In the present study, we obtained tim2 cDNA (Gb'tim2) from the cricket Gryllus bimaculatus and characterized its functional role in embryonic development, egg production, and circadian rhythms. Gb'tim2 gave rise to a 1432 amino acid protein, and showed approximately 65% homology to that of Drosophila melanogaster. When treated with parental Gb'tim2RNAi, less than 2% of the treated eggs hatched. On the other hand, control eggs treated with DsRed2RNAi demonstrated a hatching rate of 70%. In most of the Gb'tim2RNAi treated embryos, development was arrested in early stages. Egg production in ovaries of adult virgin females treated with Gb'tim2RNAi was significantly reduced. In addition, while Gb'tim2RNAi crickets exhibited clear locomotor rhythm synchronized with light cycles, their light-on peak was weaker than that of control crickets. Under constant darkness, the activity rhythm of Gb'tim2RNAi crickets was often split into two components running with different periods. Molecular analysis revealed that Gb'tim2RNAi treatment downregulated mRNA levels of Gb'per and Gb'Clk, and enhanced Gb'cyc expression rhythm
    no distinct effect was found on Gb'tim expression levels. The change in Gb'per, Gb'Clk and Gb'cyc levels may underlie the altered behavioral rhythms in Gb'tim2RNAi crickets. Both Gb'ClkRNAi and Gb'cycRNAi downregulated Gb'tim2 expression, which suggested that transcription of Gb'tim2 is mediated by Gb'CLK and Gb'CYC through E-box. These results suggested that Gb'tim2 may be involved in both reproduction and circadian rhythm regulation in crickets.

    DOI: 10.1016/j.jinsphys.2017.12.007

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  • Molecular mechanisms of limb regeneration: insights from regenerating legs of the cricket Gryllus bimaculatus. 査読 国際誌

    Tetsuya Bando, Taro Mito, Yoshimasa Hamada, Yoshiyasu Ishimaru, Sumihare Noji, Hideyo Ohuchi

    The International journal of developmental biology   62 ( 6-7-8 )   559 - 569   2018年

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    記述言語:英語  

    This review summarizes recent advances in leg regeneration research, focusing on the cricket Gryllus bimaculatus. Recent studies have revealed molecular mechanisms on blastema formation, establishment of positional information, and epigenetic regulation during leg regeneration. Especially, these studies have provided molecular bases in classical conceptual models such as the polar coordinate model, the intercalation model, the boundary model, the steepness model, etc., which were proposed to interpret regeneration processes of the cockroach legs. When a leg is amputated, a blastema is formed through the activation of the Janus-kinase (Jak)/Signal-Transduction-and-Activator-of-Transcription (STAT) pathway. Subsequently, the Hedgehog/Wingless/Decapentaplegic/Epidermal-growth-factor pathways instruct distalization in the blastema, designated as the molecular boundary model. Downstream targets of this pathway are transcription factors Distal-less (Dll) and dachshund (dac), functioning as key regulators of proximodistal pattern formation. Dll and dac specify the distal and proximal regions in the blastema, respectively, through the regulation of tarsal patterning genes. The expression of leg patterning genes during regeneration may be epigenetically controlled by histone H3K27 methylation via Enhancer-of-zeste and Ubiquitously-transcribed-tetratricopeptide-repeat-gene-X-chromosome. For the molecular mechanism of intercalation of the missing structures between the amputated position and the most distal one, Dachsous/Fat (Ds/Ft) steepness model has been proposed, in which the Ds/Ft pathway maintains positional information and determines leg size through dac expression. This model was theoretically verified to interpret the experimental results obtained with cricket legs. Availability of whole-genome sequence information, regeneration-dependent RNA interference, and genome editing technique will have the cricket be an ideal model system to reveal gene functions in leg regeneration.

    DOI: 10.1387/ijdb.180048ho

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  • A novel photic entrainment mechanism for the circadian clock in an insect: involvement of c-fos and cryptochromes

    Yuki Kutaragi, Atsushi Tokuoka, Yasuaki Tomiyama, Motoki Nose, Takayuki Watanabe, Tetsuya Bando, Yoshiyuki Moriyama, Kenji Tomioka

    Zoological Letters   4   2018年

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  • Analysis of CRISPR genome-edited founder mice shows relationship between somatic mosaicism of Pax6 mutation and variable developmental eye abnormalities

    Akihiro Yasue, Hitomi Kono, Munenori Habuta, Tetsuya Bando, Keita Sato, Junji Inoue, Seiichi Oyadomari, Sumihare Noji, Eiji Tanaka, Hideyo Ohuchi

    MECHANISMS OF DEVELOPMENT   145   S151 - S151   2017年7月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ELSEVIER SCIENCE BV  

    DOI: 10.1016/j.mod.2017.04.427

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  • cryptochrome genes form an oscillatory loop independent of the per/tim loop in the circadian clockwork of the cricket Gryllus bimaculatus

    Tokuoka, Atsushi, Itoh, Taichi Q., Hori, Shinryo, Uryu, Outa, Danbara, Yoshiki, Nose, Motoki, Bando, Tetsuya, Tanimura, Teiichi, Tomioka, Kenji

    ZOOLOGICAL LETTERS   3 ( 1 )   375 - 381   2017年4月

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    記述言語:英語   出版者・発行元:BIOMED CENTRAL LTD  

    Background: Animals exhibit circadian rhythms with a period of approximately 24 h in various physiological functions, including locomotor activity. This rhythm is controlled by an endogenous oscillatory mechanism, or circadian clock, which consists of cyclically expressed clock genes and their product proteins. cryptochrome (cry) genes are thought to be involved in the clock mechanism, and their functions have been examined extensively in holometabolous insects, but in hemimetabolous insects their role is less well understood.Results: In the present study, the role of cry genes was investigated using RNAi technology in a hemimetabolous insect, the cricket Gryllus bimaculatus. Using a molecular cloning approach, we obtained cDNAs for two cry genes: Drosophila-type cry1 (Gb ' cry1) and mammalian-type cry2 (Gb'cry2). Gb ' cry2 has six splicing variants, most of which showed rhythmic mRNA expression. Gb ' cry1(RNAi) treatment had only a limited effect at the behavioral and molecular levels, while Gb ' cry2(RNAi) had a significant effect on behavioral rhythms and molecular oscillatory machinery, alone or in combination with Gb'cry1RNAi. In Gb ' cry1/ Gb ' cry2 double-RNAi crickets, most clock genes showed arrhythmic expression, except for timeless, which retained clear rhythmic expression. Molecular analysis revealed that some combination of Gb ' cry1 and Gb ' cry2 variants suppressed CLK/CYC transcriptional activity in cultured cells.Conclusion: Based on these results, we propose a new model of the cricket's circadian clock, including a molecular oscillatory loop for Gb'cry2, which can operate independent of the Gb ' per/Gb ' tim loop.

    DOI: 10.1186/s40851-017-0066-7

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  • Expression analysis of Dickkopf-related protein 3 (Dkk3) suggests its pleiotropic roles for a secretory glycoprotein in adult mouse

    Junji Inoue, Hirofumi Fujita, Tetsuya Bando, Yoichi Kondo, Hiromi Kumon, Hideyo Ohuchi

    JOURNAL OF MOLECULAR HISTOLOGY   48 ( 1 )   29 - 39   2017年2月

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    記述言語:英語   出版者・発行元:SPRINGER  

    Dickkopf-related protein 3 (Dkk3) is the third member of the Dkk gene family and identical to the gene, whose expression was reduced in immortalized cells. Therefore, its another name is reduced expression in immortalized cells. Since the intratumoral introduction of Dkk3 inhibits tumor growth in mouse models of cancers, Dkk3 is likely a tumor suppressor gene. However, the functions of Dkk3 in vivo remain unclear. As the first step to decipher the physiological roles of this gene, we examined the expression pattern of Dkk3 in various tissues from adult mice. In situ hybridization showed that Dkk3 mRNA was detected in the brain, retina, heart, gastrointestinal tract, adrenal glands, thymus, prostate glands, seminal vesicles, testes, and ovaries in a regionally specific manner. Furthermore, we raised anti-mouse Dkk3 antibody and performed immunohistochemistry. Cytoplasmic localization of Dkk3 protein was observed in the cells of the adrenal medulla, while Dkk3 immunoreactivity was observed in the lumen of the stomach and intestine, implying that the Dkk3 protein may be secreted into the lumen of the gastrointestinal tract. These results suggest that Dkk3 has pleiotropic roles for a secretory glycoprotein that acts primarily in the gastrointestinal tract, thymus, endocrine and reproductive organs of the mouse.

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  • Expression analysis of Dickkopf-related protein 3 (Dkk3) suggests its pleiotropic roles for a secretory glycoprotein in adult mouse

    Junji Inoue, Hirofumi Fujita, Tetsuya Bando, Yoichi Kondo, Hiromi Kumon, Hideyo Ohuchi

    JOURNAL OF MOLECULAR HISTOLOGY   48 ( 1 )   29 - 39   2017年2月

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    記述言語:英語   出版者・発行元:SPRINGER  

    Dickkopf-related protein 3 (Dkk3) is the third member of the Dkk gene family and identical to the gene, whose expression was reduced in immortalized cells. Therefore, its another name is reduced expression in immortalized cells. Since the intratumoral introduction of Dkk3 inhibits tumor growth in mouse models of cancers, Dkk3 is likely a tumor suppressor gene. However, the functions of Dkk3 in vivo remain unclear. As the first step to decipher the physiological roles of this gene, we examined the expression pattern of Dkk3 in various tissues from adult mice. In situ hybridization showed that Dkk3 mRNA was detected in the brain, retina, heart, gastrointestinal tract, adrenal glands, thymus, prostate glands, seminal vesicles, testes, and ovaries in a regionally specific manner. Furthermore, we raised anti-mouse Dkk3 antibody and performed immunohistochemistry. Cytoplasmic localization of Dkk3 protein was observed in the cells of the adrenal medulla, while Dkk3 immunoreactivity was observed in the lumen of the stomach and intestine, implying that the Dkk3 protein may be secreted into the lumen of the gastrointestinal tract. These results suggest that Dkk3 has pleiotropic roles for a secretory glycoprotein that acts primarily in the gastrointestinal tract, thymus, endocrine and reproductive organs of the mouse.

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  • Relationship between somatic mosaicism of Pax6 mutation and variable developmental eye abnormalities-an analysis of CRISPR genome-edited mouse embryos

    Akihiro Yasue, Hitomi Kono, Munenori Habuta, Tetsuya Bando, Keita Sato, Junji Inoue, Seiichi Oyadomari, Sumihare Noji, Eiji Tanaka, Hideyo Ohuchi

    Scientific Reports   7 ( 1 )   1 - 13   2017年

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    記述言語:英語   出版者・発行元:Nature Publishing Group  

    The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-Associated protein (Cas) system is a rapid gene-Targeting technology that does not require embryonic stem cells. To demonstrate dosage effects of the Pax6 gene on eye formation, we generated Pax6-deficient mice with the CRISPR/Cas system. Eyes of founder embryos at embryonic day (E) 16.5 were examined and categorized according to macroscopic phenotype as class 1 (small eye with distinct pigmentation), class 2 (pigmentation without eye globes), or class 3 (no pigmentation and no eyes). Histologically, class 1 eyes were abnormally small in size with lens still attached to the cornea at E16.5. Class 2 eyes had no lens and distorted convoluted retinas. Class 3 eyes had only rudimentary optic vesicle-like tissues or histological anophthalmia. Genotyping of neck tissue cells from the founder embryos revealed somatic mosaicism and allelic complexity for Pax6. Relationships between eye phenotype and genotype were developed. The present results demonstrated that development of the lens from the surface ectoderm requires a higher gene dose of Pax6 than development of the retina from the optic vesicle. We further anticipate that mice with somatic mosaicism in a targeted gene generated by CRISPR/Cas-mediated genome editing will give some insights for understanding the complexity in human congenital diseases that occur in mosaic form.

    DOI: 10.1038/s41598-017-00088-w

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  • Transcriptional and nontranscriptional events are involved in photic entrainment of the circadian clock in the cricket Gryllus bimaculatus

    Yuki Kutaragi, Taiki Miki, Tetsuya Bando, Kenji Tomioka

    PHYSIOLOGICAL ENTOMOLOGY   41 ( 4 )   358 - 368   2016年12月

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    記述言語:英語   出版者・発行元:WILEY  

    Most insects show daily activity rhythms that are controlled by endogenous circadian clocks. A basic property of the clock is entrainment to daily environmental cycles to run with an exact 24-h period. The entrainment is achieved mainly through resetting by light. The present study analyses the light resetting mechanism of the clock in first-instar nymphal and adult crickets Gryllus bimaculatus (De Geer). A 3-h light pulse given at early subjective night and late subjective night causes a phase delay and an advance, respectively, of adult locomotor rhythms. The magnitude of the shift caused by the light pulse at circadian time 12 h in constant darkness is significantly smaller than that caused by a 3-h extension of light phase. Measurement of mRNA levels during the light-induced phase shifts yielded basically similar results for nymphs and adults. When a 3-h light pulse is given at early night by light phase extension, an increase of Pdp1 occurs, which is followed by upregulation of the genes Clk and subsequently by per and tim. These changes are eliminated by RNA interference of opsin-long wavelength, which is expressed in the compound eye and encodes a green-sensitive opsin, the major photoreceptor for photic entrainment of the clock. No clear changes in mRNA levels are observed for light pulses given at late subjective night or at early subjective night after 24 h of constant darkness. These results suggest that the photic entrainment mechanism of the clock may be shared by nymphal and adult crickets, including transcriptional and nontranscriptional events.

    DOI: 10.1111/phen.12162

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  • TGF-β signaling in insects regulates metamorphosis via juvenile hormone biosynthesis

    Yoshiyasu Ishimaru, Sayuri Tomonari, Yuji Matsuoka, Takahito Watanabe, Katsuyuki Miyawaki, Tetsuya Bando, Kenji Tomioka, Hideyo Ohuchi, Sumihare Noji, Taro Mito

    Proceedings of the National Academy of Sciences of the United States of America   113 ( 20 )   5634 - 5639   2016年5月

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  • Autopsy Case of Bilateral Optic Nerve Aplasia with Microphthalmia: Neural Retina Formation Is Required for the Coordinated Development of Ocular Tissues

    Hideyo Ohuchi, Kaori Taniguchi, Satoru Miyaishi, Hitomi Kono, Hirofumi Fujita, Tetsuya Bando, Chiharu Fuchizawa, Yuko Ohtani, Osamu Ohtani

    ACTA MEDICA OKAYAMA   70 ( 2 )   131 - 137   2016年4月

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    記述言語:英語   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    Human congenital anomalies provide information that contributes to the understanding of developmental mechanisms. Here we report bilateral optic nerve aplasia (ONA) with microphthalmia in the autopsy of the cadaver of a 70-year-old Japanese female. The gross anatomical inspection of the brain showed a cotton thread-like cord in the presumed location of the optic nerve tract or chiasm. Histologically, no neural retina, optic nerve bundle or retinal central vessels were formed in the eye globe, and the retinal pigment cells formed rosettes. The cornea, iris, and lens were also histologically abnormal. Immunohistochemically, no retinal cells expressed beta III tubulin, and Pax6-immunoreactive cells were present in the ciliary non-pigmented epithelial cells. This case of ONA could be attributed to the agenesis of retinal projection neurons as a sequel to the disruption of neural retina development. The neural retina formation would coordinate the proper development of ocular tissues.

    DOI: 10.18926/AMO/54192

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  • 昆虫に学ぶ器官再生

    板東哲哉, 濱田良真, 奥村美紗, 坂東優希, 大内淑代

    ケミカルタイムズ   241   2 - 6   2016年

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  • Enhancer of zeste plays an important role in photoperiodic modulation of locomotor rhythm in the cricket, Gryllus bimaculatus. 国際誌

    Yoshimasa Hamada, Atsushi Tokuoka, Tetsuya Bando, Hideyo Ohuchi, Kenji Tomioka

    Zoological letters   2   5 - 5   2016年

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    記述言語:英語  

    INTRODUCTION: Insects show daily behavioral rhythms controlled by an endogenous oscillator, the circadian clock. The rhythm synchronizes to daily light-dark cycles (LD) and changes waveform in association with seasonal change in photoperiod. RESULTS: To explore the molecular basis of the photoperiod-dependent changes in circadian locomotor rhythm, we investigated the role of a chromatin modifier, Enhancer of zeste (Gb'E(z)), in the cricket, Gryllus bimaculatus. Under a 12 h:12 h LD (LD 12:12), Gb'E(z) was constitutively expressed in the optic lobe, the site of the biological clock; active phase (α) and rest phase (ρ) were approximately 12 h in duration, and α/ρ ratio was approximately 1.0. When transferred to LD 20:4, the α/ρ ratio decreased significantly, and the Gb'E(z) expression level was significantly reduced at 6 h and 10 h after light-on, as was reflected in the reduced level of trimethylation of histone H3 lysine 27. This change was associated with change in clock gene expression profiles. The photoperiod-dependent changes in α/ρ ratio and clock gene expression profiles were prevented by knocking down Gb'E(z) by RNAi. CONCLUSIONS: These results suggest that histone modification by Gb'E(z) is involved in photoperiodic modulation of the G. bimaculatus circadian rhythm.

    DOI: 10.1186/s40851-016-0042-7

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  • Short germ insects utilize both the ancestral and derived mode of Polycomb group-mediated epigenetic silencing of Hox genes

    Yuji Matsuoka, Tetsuya Bando, Takahito Watanabe, Yoshiyasu Ishimaru, Sumihare Noji, Aleksandar Popadic, Taro Mito

    BIOLOGY OPEN   4 ( 6 )   702 - 709   2015年6月

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    記述言語:英語   出版者・発行元:COMPANY OF BIOLOGISTS LTD  

    In insect species that undergo long germ segmentation, such as Drosophila, all segments are specified simultaneously at the early blastoderm stage. As embryogenesis progresses, the expression boundaries of Hox genes are established by repression of gap genes, which is subsequently replaced by Polycomb group (PcG) silencing. At present, however, it is not known whether patterning occurs this way in a more ancestral (short germ) mode of embryogenesis, where segments are added gradually during posterior elongation. In this study, two members of the PcG family, Enhancer of zeste (E(z)) and Suppressor of zeste 12 (Su(z) 12), were analyzed in the short germ cricket, Gryllus bimaculatus. Results suggest that although stepwise negative regulation by gap and PcG genes is present in anterior members of the Hox cluster, it does not account for regulation of two posterior Hox genes, abdominal-A (abd-A) and Abdominal-B (Abd-B). Instead, abd-A and Abd-B are predominantly regulated by PcG genes, which is the mode present in vertebrates. These findings suggest that an intriguing transition of the PcG-mediated silencing of Hox genes may have occurred during animal evolution. The ancestral bilaterian state may have resembled the current vertebrate mode of regulation, where PcG-mediated silencing of Hox genes occurs before their expression is initiated and is responsible for the establishment of individual expression domains. Then, during insect evolution, the repression by transcription factors may have been acquired in anterior Hox genes of short germ insects, while PcG silencing was maintained in posterior Hox genes.

    DOI: 10.1242/bio.201411064

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  • Involvement of dachshund and Distal-less in distal pattern formation of the cricket leg during regeneration

    Yoshiyasu Ishimaru, Taro Nakamura, Tetsuya Bando, Yuji Matsuoka, Hideyo Ohuchi, Sumihare Noji, Taro Mito

    SCIENTIFIC REPORTS   5   8387   2015年2月

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    記述言語:英語   出版者・発行元:NATURE PUBLISHING GROUP  

    Cricket nymphs have the remarkable ability to regenerate a functional leg following amputation, indicating that the regenerating blastemal cells contain information for leg morphology. However, the molecular mechanisms that underlie regeneration of leg patterns remain poorly understood. Here, we analyzed phenotypes of the tibia and tarsus (three tarsomeres) obtained by knockdown with regeneration-dependent RNA interference (rdRNAi) against Gryllus dachshund (Gb'dac) and Distal-less (Gb'Dll). We found that depletion of Gb'Dll mRNA results in loss of the tarsal segments, while rdRNAi against Gb'dac shortens the tibia at the two most distal tarsomeres. These results indicate that Gb'Dll expression is indispensable for formation of the tarsus, while Gb'dac expression is necessary for elongation of the tibia and formation of the most proximal tarsomere. These findings demonstrate that mutual transcriptional regulation between the two is indispensable for formation of the tarsomeres, whereas Gb'dac is involved in determination of tibial size through interaction with Gb'ds/Gb'ft.

    DOI: 10.1038/srep08387

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  • Leg regeneration is epigenetically regulated by histone H3K27 methylation in the cricket Gryllus bimaculatus.

    Hamada Y, Bando T, Nakamura T, Ishimaru Y, Mito T, Noji S, Tomioka K, Ohuchi H

    Development   142 ( 17 )   2916 - 2927   2015年

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  • An extended steepness model for leg-size determination based on Dachsous/Fat trans- dimer system

    Hiroshi Yoshida, Tetsuya Bando, Taro Mito, Hideyo Ohuchi, Sumihare Noji

    SCIENTIFIC REPORTS   4   4335   2014年3月

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    記述言語:英語   出版者・発行元:NATURE PUBLISHING GROUP  

    What determines organ size has been a long-standing biological question. Lawrence et al. (2008) proposed the steepness hypothesis suggesting that the protocadherin Dachsous/Fat (Ds/Ft) system may provide some measure of dimension to the cells in relation to the gradient. In this paper we extended the model as a means of interpreting experimental results in cricket leg regeneration. We assumed that (1) Ds/Ft trans-heterodimers or trans-homodimers are redistributed during cell division, and (2) growth would cease when a differential of the dimer across each cell decreases to a certain threshold. We applied our model to simulate the results obtained by leg regeneration experiments in a cricket model. The results were qualitatively consistent with the experimental data obtained for cricket legs by RNA interference methodology. Using our extended steepness model, we provided a molecular-based explanation for leg size determination even in intercalary regeneration and for organ size determination.

    DOI: 10.1038/srep04335

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  • コオロギ脚再生の分子メカニズム

    実験医学   32 ( 1 )   15 - 21   2014年

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  • The expression of LIM-homeobox genes, Lhx1 and Lhx5, in the forebrain is essential for neural retina differentiation

    Junji Inoue, Yuuki Ueda, Tetsuya Bando, Taro Mito, Sumihare Noji, Hideyo Ohuchi

    DEVELOPMENT GROWTH & DIFFERENTIATION   55 ( 7 )   668 - 675   2013年9月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    Elucidating the mechanisms underlying eye development is essential for advancing the medical treatment of eye-related disorders. The primordium of the eye is an optic vesicle (OV), which has a dual potential for generation of the developing neural retina and retinal pigment epithelium. However, the factors that regulate the differentiation of the retinal primordium remain unclear. We have previously shown that overexpression of Lhx1 and Lhx5, members of the LIM-homeobox genes, induced the formation of a second neural retina from the presumptive pigmented retina of the OV. However, the precise timing of Lhx1 expression required for neural retina differentiation has not been clarified. Moreover, RNA interference of Lhx5 has not been previously reported. Here, using a modified electroporation method, we show that, Lhx1 expression in the forebrain around stage 8 is required for neural retina formation. In addition, we have succeeded in the knockdown of Lhx5 expression, resulting in conversion of the neural retina region to a pigment vesicle-like tissue, which indicates that Lhx5 is also required for neural retina differentiation, which correlates temporally with the activity of Lhx1. These results suggest that Lhx1 and Lhx5 in the forebrain regulate neural retina differentiation by suppressing the development of the retinal pigment epithelium, before the formation of the OV.

    DOI: 10.1111/dgd.12074

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  • Analysis of RNA-Seq data reveals involvement of JAK/STAT signalling during leg regeneration in the cricket Gryllus bimaculatus

    Tetsuya Bando, Yoshiyasu Ishimaru, Takuro Kida, Yoshimasa Hamada, Yuji Matsuoka, Taro Nakamura, Hideyo Ohuchi, Sumihare Noji, Taro Mito

    DEVELOPMENT   140 ( 5 )   959 - 964   2013年3月

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    記述言語:英語   出版者・発行元:COMPANY OF BIOLOGISTS LTD  

    In the cricket Gryllus bimaculatus, missing distal parts of the amputated leg are regenerated from the blastema, a population of dedifferentiated proliferating cells that forms at the distal tip of the leg stump. To identify molecules involved in blastema formation, comparative transcriptome analysis was performed between regenerating and normal unamputated legs. Components of JAK/STAT signalling were upregulated more than twofold in regenerating legs. To verify their involvement, Gryllus homologues of the interleukin receptor Domeless (Gb'dome), the Janus kinase Hopscotch (Gb'hop) and the transcription factor STAT (Gb'Stat) were cloned, and RNAi was performed against these genes. Gb'dome(RNAi), Gb'hop(RNAi) and Gb'Stat(RNAi) crickets showed defects in leg regeneration. Blastema expression of Gb'cyclinE was decreased in the Gb'Stat(RNAi) cricket compared with that in the control. Hyperproliferation of blastema cells caused by Gb'fat(RNAi) or Gb'warts(RNAi) was suppressed by RNAi against Gb'Stat. The results suggest that JAK/STAT signalling regulates blastema cell proliferation during leg regeneration.

    DOI: 10.1242/dev.084590

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  • Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases

    Takahito Watanabe, Hiroshi Ochiai, Tetsushi Sakuma, Hadley W. Horch, Naoya Hamaguchi, Taro Nakamura, Tetsuya Bando, Hideyo Ohuchi, Takashi Yamamoto, Sumihare Noji, Taro Mito

    NATURE COMMUNICATIONS   3   2012年8月

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    記述言語:英語   出版者・発行元:NATURE PUBLISHING GROUP  

    Hemimetabolous, or incompletely metamorphosing, insects are phylogenetically relatively basal and comprise many pests. However, the absence of a sophisticated genetic model system, or targeted gene-manipulation system, has limited research on hemimetabolous species. Here we use zinc-finger nuclease and transcription activator-like effector nuclease technologies to produce genetic knockouts in the hemimetabolous insect Gryllus bimaculatus. Following the microinjection of mRNAs encoding zinc-finger nucleases or transcription activator-like effector nucleases into cricket embryos, targeting of a transgene or endogenous gene results in sequence-specific mutations. Up to 48% of founder animals transmit disrupted gene alleles after zinc-finger nucleases microinjection compared with 17% after microinjection of transcription activator-like effector nucleases. Heterozygous offspring is selected using mutation detection assays that use a Surveyor (Cel-I) nuclease, and subsequent sibling crosses create homozygous knockout crickets. This approach is independent from a mutant phenotype or the genetic tractability of the organism of interest and can potentially be applied to manage insect pests using a non-transgenic strategy.

    DOI: 10.1038/ncomms2020

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  • Expression profiles and unc-27 mutation rescue of the striated muscle type troponin I isoform-3 in Caenorhabditis elegans

    Yasuo Takashima, Shun Kitaoka, Tetsuya Bando, Hiroaki Kagawa

    GENES & GENETIC SYSTEMS   87 ( 4 )   243 - 251   2012年8月

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    記述言語:英語   出版者・発行元:GENETICS SOC JAPAN  

    Transcription control of multiple genes in tissue- and stage-specific patterns is still of major interest. We show here that troponin I (TNT) is expressed under the control of upstream non-coding sequences and had functions as an isoform of intermediate type between pharynx and body-wall of the gene. In Caenorhabditis elegans, three striated muscle TNIs are expressed in body-wall muscles and a cardiac isoform is expressed in the pharynx. We have analyzed the gene expression mechanisms of tni-3 gene and motility function of its protein product. Promoter deletion analysis of the tni-3 gene identified muscle enhancers including the head enhancer. The CBF1/Su(H)/LAG-1-binding motif was included in the head enhancer. Yeast one-hybrid screening isolated the lag-1 clone in five candidates. Functional differences between the three striated muscle TNIs were investigated by the expression of promoter-fusion genes into tni-2/unc-27(e155) null mutant animals. The results suggest that the cis-elements in the promoters of the three genes are important for their tissue-specific expression and that from the function of TNI-3, the tni-3 gene would be an intermediate in the evolution of these genes by gene duplication. Mechanisms of tni-3 expression and its molecular function may contribute to our understanding of gene evolution and developmental programs.

    DOI: 10.1266/ggs.87.243

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  • Functional analysis of the role of eyes absent and sine oculis in the developing eye of the cricket Gryllus bimaculatus

    Akira Takagi, Kazuki Kurita, Taiki Terasawa, Taro Nakamura, Tetsuya Bando, Yoshiyuki Moriyama, Taro Mito, Sumihare Noji, Hideyo Ohuchi

    DEVELOPMENT GROWTH & DIFFERENTIATION   54 ( 2 )   227 - 240   2012年2月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    In the cricket Gryllus bimaculatus, a hemimetabolous insect, the compound eyes begin to form in the embryo and increase 56 fold in size during the postembryonic development of the nymphal stage. Retinal stem cells in the anteroventral proliferation zone (AVPZ) of the nymphal eye proliferate to increase retinal progenitors, which then differentiate to form new ommatidia in the anterior region of the eye. However, mechanisms underlying this type of eye formation have not been well elucidated yet. Here, we found that the homologues of the retinal determination transcription factor genes of eyes absent (eya) and sine oculis (so) are expressed during the cricket embryonic eye formation. eya is also expressed intensely in the AVPZ of the nymphal eye. To explore their functions, we performed knockdown by RNA interference (RNAi). Knockdown of Gbeya resulted in loss of the embryonic eye. In the nymphal eye, RNAi against Gbeya or Gbso impaired retinal morphology by apparently transforming cornea structures into head cuticle. These results imply that Gbeya and Gbso are essential for the differentiation of the retinal progenitor cells and maintaining retinal structures during eye development.

    DOI: 10.1111/j.1440-169X.2011.01325.x

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  • The advent of RNA interference in Entomology

    Taro Mito, Taro Nakamura, Tetsuya Bando, Hideyo Ohuchi, Sumihare Noji

    ENTOMOLOGICAL SCIENCE   14 ( 1 )   1 - 8   2011年1月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:WILEY-BLACKWELL  

    RNA interference (RNAi) is a cellular process by which an mRNA is targeted for degradation by a small interfering RNA that contains a strand complementary to a fragment of the target mRNA, resulting in sequence specific inhibition of gene expression. The discovery of RNAi enabled the use of loss-of-function analyses in many non-model insects other than Drosophila to elucidate the roles of specific genes. The RNAi approach has been widely used on insects in several fields, including embryogenesis, pattern formation, reproduction, biosynthesis and behavior. The increasing availability of insect genomes has made the RNAi technique an indispensable technique for characterizing gene functions in insects. Here we review the current status of RNAi-based experiments in insects and the applications of RNAi for species-specific insecticides, focusing on non-drosophilid insects. We also identify future applications for RNAi-based studies in Entomology.

    DOI: 10.1111/j.1479-8298.2010.00408.x

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  • Dynamics of cellular aggregation and differentiation during early development of transgenic crickets

    Taro Nakamura, Taro Mito, Masato Yoshizaki, Tetsuya Bando, Kimio Tanaka, Hideyo Ohuchi, Sumihare Noji

    MECHANISMS OF DEVELOPMENT   126   S254 - S254   2009年8月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ELSEVIER SCIENCE BV  

    DOI: 10.1016/j.mod.2009.06.666

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  • Involvement of the Hedgehog/Engrailed signaling in regeneration of the posterior structures in the amputated leg of the cricket Gryllus bimaculatus

    Katsuyuki Miyawaki, Taro Mito, Taro Nakamura, Tetsuya Bando, Kimio Tanaka, Hideyo Ohuchi, Sumihare Noji

    MECHANISMS OF DEVELOPMENT   126   S294 - S294   2009年8月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ELSEVIER SCIENCE BV  

    DOI: 10.1016/j.mod.2009.06.799

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  • Regulation of leg size and shape by the Dachsous/Fat signalling pathway during regeneration in Gryllus bimaculatus

    Tetsuya Bando, Taro Mito, Yuko Maeda, Taro Nakamura, Fumiaki Ito, Takahito Watanabe, Hideyo Ohuchi, Sumihare Noji

    MECHANISMS OF DEVELOPMENT   126   S293 - S294   2009年8月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ELSEVIER SCIENCE BV  

    DOI: 10.1016/j.mod.2009.06.797

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  • ゲノムの地理と歴史―非コード領域の生物学的機能― 査読

    香川弘昭, 板東哲哉, 高島康郎

    生物物理   49 ( 5 )   246 - 248   2009年

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  • Warts, Expanded and Dachs are required for proper regeneration of amputated legs in the cricket, Gryllus bimaculatus

    Yuko Maeda, Tetsuya Bando, Taro Nakamura, Taro Mito, Hideyo Ohuchi, Sumihare Noji

    GENES & GENETIC SYSTEMS   82 ( 6 )   532 - 532   2007年12月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:GENETICS SOC JAPAN  

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  • Fat and dachsous protocadherin are required for proper proximodistal and circumferential regeneration of amputated legs in the cricket, Gryllus bimaculatus

    Tetsuya Bando, Yuko Maeda, Taro Nakamura, Taro Mito, Hideyo Ohuchi, Sumihare Noji

    GENES & GENETIC SYSTEMS   82 ( 6 )   532 - 532   2007年12月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:GENETICS SOC JAPAN  

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講演・口頭発表等

  • 再生モデル昆虫ではToll様受容体が器官再生を促進する

    第123回 日本解剖学会総会・全国学術集会  2018年 

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  • Innate immunity signaling pathways promote leg regeneration in the cricket

    第70回日本細胞生物学会 第51回日本発生生物学会 合同大会  2018年 

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  • 再生モデル昆虫における自然免疫シグナル経路を介した器官再生の促進メカニズム

    ConBio2017  2017年 

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  • 再生モデル昆虫におけるマクロファージを介した再生開始メカニズム

    第122回 日本解剖学会総会・全国学術集会  2017年 

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  • がん関連遺伝子 Dickkopf-3(Dkk-3)の生体内における発現パターンの解析

    第57回日本組織細胞化学会総会・学術集会  2016年 

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  • 再生モデル昆虫における器官再生のメカニズム

    第121回 日本解剖学会総会・全国学術集会  2016年 

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  • フタホシコオロギ脚再生におけるマクロファージを中心とした炎症反応の役割

    第2回 ユニークな少数派実験動物を扱う若手が最先端アプローチを勉強する会  2016年 

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  • Molecular link between innate immunity and tissue regeneration

    Cricket Meeting 2016  2016年 

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  • Angiomotin regulates cell proliferation cooperatively with Expanded and Merlin during cricket leg regeneration

    第38回 日本分子生物学会年会  2015年 

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  • Cyclin B3 is involved in leg regeneration of the cricket Gryllus bimaculatus

    第120回 日本解剖学会総会・全国学術集会  2015年 

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  • Epigenetic regulation on histone H3K27 is involved in redifferentiation process during leg regeneration in the cricket Gryllus bimaculatus

    第120回 日本解剖学会総会・全国学術集会  2015年 

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  • ポリプテルス眼の発生分化と機能に関わる遺伝子群

    第5回 Tokyo Vertebrate Morphology Meeting  2015年 

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  • 再生モデル昆虫コオロギに学ぶ器官再生のメカニズム

    再生生物学シンポジウム『次世代型器官再生生物学の発展』  2015年 

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  • コオロギ脚再生において再生芽細胞の再分化はヒストンH3K27メチル化により制御される

    第37回 日本分子生物学会年会  2014年 

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  • CRISPR/CasシステムによるPax6遺伝子破壊マウスの解析

    第37回 日本分子生物学会年会  2014年 

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  • コオロギにおけるエピジェネティック因子E(z)の概日リズムおよび再生への関与

    日本動物学会 第85回大会  2014年 

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  • コオロギに学ぶ器官再生の分子メカニズム

    第119回 日本解剖学会総会・全国学術集会  2014年 

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  • Molecular basis on tissue regeneration in the cricket as revealed by RNA interference

    International Symposium on RNAi and Genome Editing Research  2014年 

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  • Mechanisms underlying regeneration of the cricket leg, based on an extended steepness model

    EMBO Conference The Molecular and cellular basis of regeneration and tissue repair  2014年 

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  • Epigenetic regulation of genes expressions via methylation on histone H3 27th lysine residue during leg regeneration

    第47回 日本発生生物学会  2014年 

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  • Epigenetic regulation of genes expressions via methylation on histone H3K27 during leg regeneration in the cricket Gryllus bimaculatus

    CDBシンポジウム2014 Regeneration of Organs: Programming and Self-Organization  2014年 

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  • 再生モデル昆虫を用いた器官再生の分子基盤の研究

    第12回日本再生医療学会総会  2013年 

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  • 再生を制御する普遍的分子機構の解明:昆虫の脚再生からヒト器官再生を目指して

    日本解剖学会 第68回中国・四国支部学術集会  2013年 

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  • フタホシコオロギ脚再生過程におけるパターン形成遺伝子のエピジェネティックな発現制御

    日本動物学会第84回大会  2013年 

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  • 器官再生において再生芽細胞の再分化を制御するエピジェネティックな機構

    日本分子生物学会第36回年会  2013年 

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  • JAK/STAT signaling promotes blastemal cell proliferation during leg regeneration in the cricket Gryllus bimaculatus

    第46回日本発生生物学会  2013年 

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  • Molecular mechanism for regulation of blastema cell proliferation during leg regeneration in Gryllus bimaculatus

    2012年 

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  • コオロギ脚再生において再生芽細胞の増殖を制御する分子メカニズムの解析

    第35回日本分子生物学会年会  2012年 

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担当授業科目

  • 人体の構造:入門 (2021年度) 特別  - その他

  • 人体構造学 (2021年度) 集中  - その他

  • 発生学 (2021年度) 特別  - その他

  • 細胞組織学 (2021年度) 特別  - その他

  • 細胞組織学実習 (2021年度) 特別  - その他

  • 細胞組織学I(演習・実習) (2021年度) 特別  - その他

  • 細胞組織学I(講義・演習) (2021年度) 特別  - その他

  • 細胞組織学II(演習・実習) (2021年度) 特別  - その他

  • 細胞組織学II(講義・演習) (2021年度) 特別  - その他

  • 人体の構造:入門 (2020年度) 特別  - その他

  • 人体構造学 (2020年度) 集中  - その他

  • 発生学 (2020年度) 特別  - その他

  • 細胞組織学 (2020年度) 特別  - その他

  • 細胞組織学実習 (2020年度) 特別  - その他

  • 細胞組織学I(演習・実習) (2020年度) 特別  - その他

  • 細胞組織学I(講義・演習) (2020年度) 特別  - その他

  • 細胞組織学II(演習・実習) (2020年度) 特別  - その他

  • 細胞組織学II(講義・演習) (2020年度) 特別  - その他

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