2024/10/18 更新

写真a

バンドウ テツヤ
板東 哲哉
BANDO Tetsuya
所属
医歯薬学域 講師
職名
講師
外部リンク

学位

  • 博士(理学) ( 岡山大学 )

研究キーワード

  • Regeneration

  • 器官再生

研究分野

  • ライフサイエンス / ゲノム生物学

  • ライフサイエンス / 発生生物学

  • ライフサイエンス / システムゲノム科学

学歴

  • 岡山大学    

    - 2005年

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    国名: 日本国

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  • 岡山大学    

    - 1999年

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    国名: 日本国

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  • 岡山大学   Faculty of Science   Department of Biology

    - 1997年

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    国名: 日本国

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経歴

  • - Senior Assistant Professor,Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,Okayama University

    2016年 - 現在

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  • - 岡山大学医歯薬学総合研究科 講師

    2016年 - 現在

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  • Assistant Professor,Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,Okayama University

    2012年 - 2016年

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  • 岡山大学医歯薬学総合研究科 助教

    2012年 - 2016年

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  • Researcher

    2009年 - 2012年

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  • 徳島大学

    2009年 - 2012年

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  • Researcher

    2008年 - 2009年

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  • 科学技術振興機構 研究員

    2008年 - 2009年

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  • Researcher

    2005年 - 2008年

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  • 徳島大学

    2005年 - 2008年

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▼全件表示

所属学協会

 

論文

  • Toll signalling promotes blastema cell proliferation during cricket leg regeneration via insect macrophages 査読 国際誌

    Tetsuya Bando, Misa Okumura, Yuki Bando, Marou Hagiwara, Yoshimasa Hamada, Yoshiyasu Ishimaru, Taro Mito, Eri Kawaguchi, Takeshi Inoue, Kiyokazu Agata, Sumihare Noji, Hideyo Ohuchi

    Development   149 ( 8 )   2022年4月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:The Company of Biologists  

    <title>ABSTRACT</title>
    Hemimetabolous insects, such as the two-spotted cricket Gryllus bimaculatus, can recover lost tissues, in contrast to the limited regenerative abilities of human tissues. Following cricket leg amputation, the wound surface is covered by the wound epidermis, and plasmatocytes, which are insect macrophages, accumulate in the wound region. Here, we studied the function of Toll-related molecules identified by comparative RNA sequencing during leg regeneration. Of the 11 Toll genes in the Gryllus genome, expression of Toll2-1, Toll2-2 and Toll2-5 was upregulated during regeneration. RNA interference (RNAi) of Toll, Toll2-1, Toll2-2, Toll2-3 or Toll2-4 produced regeneration defects in more than 50% of crickets. RNAi of Toll2-2 led to a decrease in the ratio of S- and M-phase cells, reduced expression of JAK/STAT signalling genes, and reduced accumulation of plasmatocytes in the blastema. Depletion of plasmatocytes in crickets using clodronate also produced regeneration defects, as well as fewer proliferating cells in the regenerating legs. Plasmatocyte depletion also downregulated the expression of Toll and JAK/STAT signalling genes in the regenerating legs. These results suggest that Spz-Toll-related signalling in plasmatocytes promotes leg regeneration through blastema cell proliferation by regulating the Upd-JAK/STAT signalling pathway.

    DOI: 10.1242/dev.199916

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    その他リンク: https://journals.biologists.com/dev/article-pdf/doi/10.1242/dev.199916/2119087/dev199916.pdf

  • Insights into the genomic evolution of insects from cricket genomes 査読 国際共著 国際誌

    Guillem Ylla, Taro Nakamura, Takehiko Itoh, Rei Kajitani, Atsushi Toyoda, Sayuri Tomonari, Tetsuya Bando, Yoshiyasu Ishimaru, Takahito Watanabe, Masao Fuketa, Yuji Matsuoka, Austen A. Barnett, Sumihare Noji, Taro Mito, Cassandra G. Extavour

    Communications Biology   4 ( 1 )   2021年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    <title>Abstract</title>Most of our knowledge of insect genomes comes from Holometabolous species, which undergo complete metamorphosis and have genomes typically under 2 Gb with little signs of DNA methylation. In contrast, Hemimetabolous insects undergo the presumed ancestral process of incomplete metamorphosis, and have larger genomes with high levels of DNA methylation. Hemimetabolous species from the Orthopteran order (grasshoppers and crickets) have some of the largest known insect genomes. What drives the evolution of these unusual insect genome sizes, remains unknown. Here we report the sequencing, assembly and annotation of the 1.66-Gb genome of the Mediterranean field cricket <italic>Gryllus bimaculatus</italic>, and the annotation of the 1.60-Gb genome of the Hawaiian cricket <italic>Laupala kohalensis</italic>. We compare these two cricket genomes with those of 14 additional insects and find evidence that hemimetabolous genomes expanded due to transposable element activity. Based on the ratio of observed to expected CpG sites, we find higher conservation and stronger purifying selection of methylated genes than non-methylated genes. Finally, our analysis suggests an expansion of the <italic>pickpocket</italic> class V gene family in crickets, which we speculate might play a role in the evolution of cricket courtship, including their characteristic chirping.

    DOI: 10.1038/s42003-021-02197-9

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    その他リンク: http://www.nature.com/articles/s42003-021-02197-9

  • Molecular mechanisms of limb regeneration: insights from regenerating legs of the cricket Gryllus bimaculatus. 査読 国際誌

    Tetsuya Bando, Taro Mito, Yoshimasa Hamada, Yoshiyasu Ishimaru, Sumihare Noji, Hideyo Ohuchi

    The International journal of developmental biology   62 ( 6-7-8 )   559 - 569   2018年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    This review summarizes recent advances in leg regeneration research, focusing on the cricket Gryllus bimaculatus. Recent studies have revealed molecular mechanisms on blastema formation, establishment of positional information, and epigenetic regulation during leg regeneration. Especially, these studies have provided molecular bases in classical conceptual models such as the polar coordinate model, the intercalation model, the boundary model, the steepness model, etc., which were proposed to interpret regeneration processes of the cockroach legs. When a leg is amputated, a blastema is formed through the activation of the Janus-kinase (Jak)/Signal-Transduction-and-Activator-of-Transcription (STAT) pathway. Subsequently, the Hedgehog/Wingless/Decapentaplegic/Epidermal-growth-factor pathways instruct distalization in the blastema, designated as the molecular boundary model. Downstream targets of this pathway are transcription factors Distal-less (Dll) and dachshund (dac), functioning as key regulators of proximodistal pattern formation. Dll and dac specify the distal and proximal regions in the blastema, respectively, through the regulation of tarsal patterning genes. The expression of leg patterning genes during regeneration may be epigenetically controlled by histone H3K27 methylation via Enhancer-of-zeste and Ubiquitously-transcribed-tetratricopeptide-repeat-gene-X-chromosome. For the molecular mechanism of intercalation of the missing structures between the amputated position and the most distal one, Dachsous/Fat (Ds/Ft) steepness model has been proposed, in which the Ds/Ft pathway maintains positional information and determines leg size through dac expression. This model was theoretically verified to interpret the experimental results obtained with cricket legs. Availability of whole-genome sequence information, regeneration-dependent RNA interference, and genome editing technique will have the cricket be an ideal model system to reveal gene functions in leg regeneration.

    DOI: 10.1387/ijdb.180048ho

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  • Leg formation and regeneration 査読

    Tetsuya Bando, Yoshimasa Hamada, Sumihare Noji

    The Cricket as a Model Organism: Development, Regeneration, and Behavior   31 - 48   2017年1月

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    掲載種別:論文集(書籍)内論文  

    © Springer Japan KK 2017. All rights reserved. In contrast to higher vertebrates, orthopteran nymphs have remarkable regenerative capacity for regrowing complex morphological structures and organs. In this review, we summarize the molecular basis of tissue regeneration in the cricket Gryllus bimaculatus. In this species, the lost part of a leg can be regenerated epimorphically from blastema cells, a population of dedifferentiated proliferating cells. Blastema cell proliferation is regulated by JAK/STAT and Salvador/Warts/Hippo signaling pathways. The positional information for leg regrowth, which includes the recognition of amputated position and proper regeneration, is maintained by Dachsous/Fat signaling. The regrowth of lost leg segments is reconstructed through the expressions of genes in the hedgehog, wingless, decapentaplegic, and Egf signaling pathways and epigenetic modifiers E(z) and Utx. The insights obtained reveal the high level of conservation between insects and vertebrates, suggesting that Gryllus may be a suitable model for human regenerative medicine studies.

    DOI: 10.1007/978-4-431-56478-2_3

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  • Enhancer of zeste plays an important role in photoperiodic modulation of locomotor rhythm in the cricket, Gryllus bimaculatus. 国際誌

    Yoshimasa Hamada, Atsushi Tokuoka, Tetsuya Bando, Hideyo Ohuchi, Kenji Tomioka

    Zoological letters   2   5 - 5   2016年

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    記述言語:英語  

    INTRODUCTION: Insects show daily behavioral rhythms controlled by an endogenous oscillator, the circadian clock. The rhythm synchronizes to daily light-dark cycles (LD) and changes waveform in association with seasonal change in photoperiod. RESULTS: To explore the molecular basis of the photoperiod-dependent changes in circadian locomotor rhythm, we investigated the role of a chromatin modifier, Enhancer of zeste (Gb'E(z)), in the cricket, Gryllus bimaculatus. Under a 12 h:12 h LD (LD 12:12), Gb'E(z) was constitutively expressed in the optic lobe, the site of the biological clock; active phase (α) and rest phase (ρ) were approximately 12 h in duration, and α/ρ ratio was approximately 1.0. When transferred to LD 20:4, the α/ρ ratio decreased significantly, and the Gb'E(z) expression level was significantly reduced at 6 h and 10 h after light-on, as was reflected in the reduced level of trimethylation of histone H3 lysine 27. This change was associated with change in clock gene expression profiles. The photoperiod-dependent changes in α/ρ ratio and clock gene expression profiles were prevented by knocking down Gb'E(z) by RNAi. CONCLUSIONS: These results suggest that histone modification by Gb'E(z) is involved in photoperiodic modulation of the G. bimaculatus circadian rhythm.

    DOI: 10.1186/s40851-016-0042-7

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  • Short germ insects utilize both the ancestral and derived mode of Polycomb group-mediated epigenetic silencing of Hox genes

    Yuji Matsuoka, Tetsuya Bando, Takahito Watanabe, Yoshiyasu Ishimaru, Sumihare Noji, Aleksandar Popadic, Taro Mito

    BIOLOGY OPEN   4 ( 6 )   702 - 709   2015年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:COMPANY OF BIOLOGISTS LTD  

    In insect species that undergo long germ segmentation, such as Drosophila, all segments are specified simultaneously at the early blastoderm stage. As embryogenesis progresses, the expression boundaries of Hox genes are established by repression of gap genes, which is subsequently replaced by Polycomb group (PcG) silencing. At present, however, it is not known whether patterning occurs this way in a more ancestral (short germ) mode of embryogenesis, where segments are added gradually during posterior elongation. In this study, two members of the PcG family, Enhancer of zeste (E(z)) and Suppressor of zeste 12 (Su(z) 12), were analyzed in the short germ cricket, Gryllus bimaculatus. Results suggest that although stepwise negative regulation by gap and PcG genes is present in anterior members of the Hox cluster, it does not account for regulation of two posterior Hox genes, abdominal-A (abd-A) and Abdominal-B (Abd-B). Instead, abd-A and Abd-B are predominantly regulated by PcG genes, which is the mode present in vertebrates. These findings suggest that an intriguing transition of the PcG-mediated silencing of Hox genes may have occurred during animal evolution. The ancestral bilaterian state may have resembled the current vertebrate mode of regulation, where PcG-mediated silencing of Hox genes occurs before their expression is initiated and is responsible for the establishment of individual expression domains. Then, during insect evolution, the repression by transcription factors may have been acquired in anterior Hox genes of short germ insects, while PcG silencing was maintained in posterior Hox genes.

    DOI: 10.1242/bio.201411064

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  • Leg regeneration is epigenetically regulated by histone H3K27 methylation in the cricket Gryllus bimaculatus.

    Hamada Y, Bando T, Nakamura T, Ishimaru Y, Mito T, Noji S, Tomioka K, Ohuchi H

    Development   142 ( 17 )   2916 - 2927   2015年

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    記述言語:英語   出版者・発行元:Company of Biologists  

    Hemimetabolous insects such as the cricket Gryllus bimaculatus regenerate lost tissue parts using blastemal cells, a population of dedifferentiated proliferating cells. The expression of several factors that control epigenetic modification is upregulated in the blastema compared with differentiated tissue, suggesting that epigenetic changes in gene expression might control the differentiation status of blastema cells during regeneration. To clarify the molecular basis of epigenetic regulation during regeneration, we focused on the function of the Gryllus Enhancer of zeste [Gb'E(z)] and Ubiquitously transcribed tetratricopeptide repeat gene on the X chromosome (Gb'Utx) homologues, which regulate methylation and demethylation of histone H3 lysine 27 (H3K27), respectively. Methylated histone H3K27 in the regenerating leg was diminished by Gb'E(z)RNAi and was increased by Gb'UtxRNAi. Regenerated Gb'E(z)RNAi cricket legs exhibited extra leg segment formation between the tibia and tarsus, and regenerated Gb'UtxRNAi cricket legs showed leg joint formation defects in the tarsus. In the Gb'E(z)RNAi regenerating leg, the Gb'dac expression domain expanded in the tarsus. By contrast, in the Gb'UtxRNAi regenerating leg, Gb'Egfr expression in the middle of the tarsus was diminished. These results suggest that regulation of the histone H3K27 methylation state is involved in the repatterning process during leg regeneration among cricket species via the epigenetic regulation of leg patterning gene expression.

    DOI: 10.1242/dev

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  • Analysis of RNA-Seq data reveals involvement of JAK/STAT signalling during leg regeneration in the cricket Gryllus bimaculatus

    Tetsuya Bando, Yoshiyasu Ishimaru, Takuro Kida, Yoshimasa Hamada, Yuji Matsuoka, Taro Nakamura, Hideyo Ohuchi, Sumihare Noji, Taro Mito

    DEVELOPMENT   140 ( 5 )   959 - 964   2013年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:COMPANY OF BIOLOGISTS LTD  

    In the cricket Gryllus bimaculatus, missing distal parts of the amputated leg are regenerated from the blastema, a population of dedifferentiated proliferating cells that forms at the distal tip of the leg stump. To identify molecules involved in blastema formation, comparative transcriptome analysis was performed between regenerating and normal unamputated legs. Components of JAK/STAT signalling were upregulated more than twofold in regenerating legs. To verify their involvement, Gryllus homologues of the interleukin receptor Domeless (Gb'dome), the Janus kinase Hopscotch (Gb'hop) and the transcription factor STAT (Gb'Stat) were cloned, and RNAi was performed against these genes. Gb'dome(RNAi), Gb'hop(RNAi) and Gb'Stat(RNAi) crickets showed defects in leg regeneration. Blastema expression of Gb'cyclinE was decreased in the Gb'Stat(RNAi) cricket compared with that in the control. Hyperproliferation of blastema cells caused by Gb'fat(RNAi) or Gb'warts(RNAi) was suppressed by RNAi against Gb'Stat. The results suggest that JAK/STAT signalling regulates blastema cell proliferation during leg regeneration.

    DOI: 10.1242/dev.084590

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  • Lowfat, a Mammalian Lix1 Homologue, Regulates Leg Size and Growth Under the Dachsous/Fat Signaling Pathway During Tissue Regeneration 査読

    Tetsuya Bando, Yoshimasa Hamada, Kazuki Kurita, Taro Nakamura, Taro Mito, Hideyo Ohuchi, Sumihare Noji

    DEVELOPMENTAL DYNAMICS   240 ( 6 )   1440 - 1453   2011年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    In the cricket Gryllus bimaculatus, missing distal parts of amputated legs are regenerated from blastemas based on positional information. The Dachsous/Fat (Ds/Ft) signaling pathway regulates blastema cell proliferation and positional information along the longitudinal axis during leg regeneration. Herein, we show that the Gryllus homologue of Lowfat (Gb&apos;Lft), which modulates Ds/Ft signaling in Drosophila, is involved in leg regeneration. Gb&apos;lft is expressed in regenerating legs, and RNAi against Gb&apos;lft (Gb&apos;lft RNAi) suppressed blastema cell hyperproliferation caused by Gb&apos;ft(RNAi) or Gb&apos;ds(RNAi) but enhanced that caused by Gb&apos;kibra(RNAi) or Gb&apos;warts(RNAi). In Gb&apos;lft RNAi nymphs, missing parts of amputated legs were regenerated, but the length of the regenerated legs was shortened depending on the position of the amputation. Both normal and reversed intercalary regeneration occurred in Gb&apos;lft(RNAi) nymphs, suggesting that Gb&apos;Lft is involved in blastema cell proliferation and longitudinal leg regeneration under the Ds/Ft signaling pathway, but it is not required for intercalary regeneration. Developmental Dynamics 240:1440-1453, 2011. (C) 2011 Wiley-Liss, Inc.

    DOI: 10.1002/dvdy.22647

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  • Regulation of leg size and shape by the Dachsous/Fat signalling pathway during regeneration 査読

    Tetsuya Bando, Taro Mito, Yuko Maeda, Taro Nakamura, Fumiaki Ito, Takahito Watanabe, Hideyo Ohuchi, Sumihare Noji

    DEVELOPMENT   136 ( 13 )   2235 - 2245   2009年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:COMPANY OF BIOLOGISTS LTD  

    An amputated cricket leg regenerates all missing parts with normal size and shape, indicating that regenerating blastemal cells are aware of both their position and the normal size of the leg. However, the molecular mechanisms regulating this process remain elusive. Here, we use a cricket model to show that the Dachsous/Fat (Ds/Ft) signalling pathway is essential for leg regeneration. We found that knockdown of ft or ds transcripts by regeneration-dependent RNA interference (rdRNAi) suppressed proliferation of the regenerating cells along the proximodistal (PD) axis concomitantly with remodelling of the pre-existing stump, making the regenerated legs shorter than normal. By contrast, knockdown of the expanded (ex) or Merlin (Mer) transcripts induced over-proliferation of the regenerating cells, making the regenerated legs longer. These results are consistent with those obtained using rdRNAi during intercalary regeneration induced by leg transplantation. We present a model to explain our results in which the steepness of the Ds/Ft gradient controls growth along the PD axis of the regenerating leg.

    DOI: 10.1242/dev.035204

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  • The homeoproteins MAB-18 and CEH-14 insulate the dauer collagen gene col-43 from activation by the adjacent promoter of the Spermatheca gene sth-1 in Caenorhabditis elegans. 国際誌

    Tetsuya Bando, Tatsuji Ikeda, Hiroaki Kagawa

    Journal of molecular biology   348 ( 1 )   101 - 12   2005年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Genome searches in this study indicate that the nematode Caenorhabditis elegans genome has 2582 bidirectionally oriented genes that account for more than 25% of the total genes. We analyze the transcriptional repression system for one of these predicted bidirectional promoters, which controls the expression of the spermathecal gene sth-1 and collagen gene col-43. These two genes are separated by 1.3 kb and are transcribed bidirectionally. sth-1 is expressed in spermatheca after the L4 stage and col-43 is expressed in the hypodermal cells of the L2d dauer stage. The upstream regions required for the expression of sth-1 and col-43 shared an overlapped control sequence. Two homeoproteins, MAB-18 and CEH-14, were isolated by yeast one-hybrid screening as binding proteins of the overlapped region. MAB-18 bound to two homeodomain-binding sites and interacted with CEH-14 to repress col-43 expression in spermatheca. These results indicate that the two homeoproteins interact with each other to repress col-43 expression in sth-1-expressing tissues. This is the first report of bidirectional gene regulation analysis in the C.elegans genome.

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  • Involvement of a Basic Helix-Loop-Helix Gene BHLHE40 in Specification of Chicken Retinal Pigment Epithelium 査読 国際誌

    Toshiki Kinuhata, Keita Sato, Tetsuya Bando, Taro Mito, Satoru Miyaishi, Tsutomu Nohno, Hideyo Ohuchi

    Journal of Developmental Biology   10 ( 4 )   45 - 45   2022年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MDPI AG  

    The first event of differentiation and morphogenesis in the optic vesicle (OV) is specification of the neural retina (NR) and retinal pigment epithelium (RPE), separating the inner and outer layers of the optic cup, respectively. Here, we focus on a basic helix-loop-helix gene, BHLHE40, which has been shown to be expressed by the developing RPE in mice and zebrafish. Firstly, we examined the expression pattern of BHLHE40 in the developing chicken eye primordia by in situ hybridization. Secondly, BHLHE40 overexpression was performed with in ovo electroporation and its effects on optic cup morphology and expression of NR and RPE marker genes were examined. Thirdly, we examined the expression pattern of BHLHE40 in LHX1-overexpressed optic cup. BHLHE40 expression emerged in a subset of cells of the OV at Hamburger and Hamilton stage 14 and became confined to the outer layer of the OV and the ciliary marginal zone of the retina by stage 17. BHLHE40 overexpression in the prospective NR resulted in ectopic induction of OTX2 and repression of VSX2. Conversely, BHLHE40 was repressed in the second NR after LHX1 overexpression. These results suggest that emergence of BHLHE40 expression in the OV is involved in initial RPE specification and that BHLHE40 plays a role in separation of the early OV domains by maintaining OTX2 expression and antagonizing an NR developmental program.

    DOI: 10.3390/jdb10040045

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  • The role of clockwork orange in the circadian clock of the cricket Gryllus bimaculatus 査読 国際誌

    Yasuaki Tomiyama, Tsugumichi Shinohara, Mirai Matsuka, Tetsuya Bando, Taro Mito, Kenji Tomioka

    Zoological Letters   6 ( 1 )   2020年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    <title>Abstract</title>
    The circadian clock generates rhythms of approximately 24 h through periodic expression of the clock genes. In insects, the major clock genes <italic>period</italic> (<italic>per</italic>) and <italic>timeless</italic> (<italic>tim</italic>) are rhythmically expressed upon their transactivation by CLOCK/CYCLE, with peak levels in the early night. In <italic>Drosophila</italic>, <italic>clockwork orange</italic> (<italic>cwo</italic>) is known to inhibit the transcription of <italic>per</italic> and <italic>tim</italic> during the daytime to enhance the amplitude of the rhythm, but its function in other insects is largely unknown. In this study, we investigated the role of <italic>cwo</italic> in the clock mechanism of the cricket <italic>Gryllus bimaculatus</italic>. The results of quantitative RT-PCR showed that under a light/dark (LD) cycle, <italic>cwo</italic> is rhythmically expressed in the optic lobe (lamina-medulla complex) and peaks during the night. When <italic>cwo</italic> was knocked down via RNA interference (RNAi), some crickets lost their locomotor rhythm, while others maintained a rhythm but exhibited a longer free-running period under constant darkness (DD). In <italic>cwo</italic>RNAi crickets, all clock genes except for <italic>cryptochrome 2</italic> (<italic>cry2</italic>) showed arrhythmic expression under DD; under LD, some of the clock genes showed higher mRNA levels, and <italic>tim</italic> showed rhythmic expression with a delayed phase. Based on these results, we propose that <italic>cwo</italic> plays an important role in the cricket circadian clock.

    DOI: 10.1186/s40851-020-00166-4

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    その他リンク: http://link.springer.com/article/10.1186/s40851-020-00166-4/fulltext.html

  • Functional analysis of Pdp1 and vrille in the circadian system of a cricket

    Yumina Narasaki-Funo, Yasuaki Tomiyama, Motoki Nose, Tetsuya Bando, Kenji Tomioka

    Journal of Insect Physiology   127   2020年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier Ltd  

    Circadian rhythms are generated by a circadian clock for which oscillations are based on the rhythmic expression of the so-called clock genes. The present study investigated the role of Gryllus bimaculatus vrille (Gb'vri) and Par domain protein 1 (Gb'Pdp1) in the circadian clock of the cricket Gryllus bimaculatus. Structural analysis of Gb'vri and Gb'Pdp1 cDNAs revealed that they are a member of the bZIP transcription factors. Under light/dark cycles (LD) both genes were rhythmically expressed in the clock tissue, the optic lobes, whereas the rhythm diminished under constant darkness (DD). Gb'vri and Gb'Pdp1 mRNA levels were significantly reduced by RNA interference (RNAi) of Gb'Clk and Gb'cyc, suggesting they are controlled by Gb'CLK/Gb'CYC. RNAi of Gb'vri and Gb'Pdp1 had little effect on locomotor rhythms, although their effects became visible when treated together with Gb'cycRNAi. The average free-running period of Gb'vriRNAi/Gb'cycRNAi crickets was significantly shorter than that of Gb'cycRNAi crickets. A similar period shortening was observed also when treated with Gb'Pdp1RNAi/Gb'cycRNAi. Some Gb'Pdp1RNAi/Gb'cycRNAi crickets showed rhythm splitting into two free-running components with different periods. Gb'vriRNAi and Gb'Pdp1RNAi treatments significantly altered the expression of Gb'Clk, Gb'cyc, and Gb'tim in LD. These results suggest that Gb'vri and Gb'Pdp1 play important roles in cricket circadian clocks.

    DOI: 10.1016/j.jinsphys.2020.104156

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  • Fgf10-CRISPR mosaic mutants demonstrate the gene dose-related loss of the accessory lobe and decrease in the number of alveolar type 2 epithelial cells in mouse lung 査読 国際誌

    Munenori Habuta, Akihiro Yasue, Ken-ichi T. Suzuki, Hirofumi Fujita, Keita Sato, Hitomi Kono, Ayuko Takayama, Tetsuya Bando, Satoru Miyaishi, Seiichi Oyadomari, Eiji Tanaka, Hideyo Ohuchi

    PLOS ONE   15 ( 10 )   e0240333 - e0240333   2020年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Public Library of Science (PLoS)  

    DOI: 10.1371/journal.pone.0240333

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  • Dkk3/REIC, an N-glycosylated Protein, Is a Physiological Endoplasmic Reticulum Stress Inducer in the Mouse Adrenal Gland. 査読

    Hirofumi Fujita, Tetsuya Bando, Seiichi Oyadomari, Kazuhiko Ochiai, Masami Watanabe, Hiromi Kumon, Hideyo Ohuchi

    Acta medica Okayama   74 ( 3 )   199 - 208   2020年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Dickkopf 3 (Dkk3) is a secreted protein belonging to the Dkk family and encoded by the orthologous gene of REIC. Dkk3/REIC is expressed by mouse and human adrenal glands, but the understanding of its roles in this organ is still limited. To determine the functions of Dkk3 in the mouse adrenal gland, we first identified that the mouse Dkk3 protein is N-glycosylated in the adrenal gland as well as in the brain. We performed proteome analysis on adrenal glands from Dkk3-null mice, in which exons 5 and 6 of the Dkk3 gene are deleted. Twodimensional polyacrylamide gel electrophoresis of adrenal proteins from wild-type and Dkk3-null mice revealed 5 protein spots whose intensities were altered between the 2 genotypes. Mass spectrometry analysis of these spots identified binding immunoglobulin protein (BiP), an endoplasmic reticulum (ER) chaperone. To determine whether mouse Dkk3 is involved in the unfolded protein response (UPR), we carried out a reporter assay using ER-stress responsive elements. Forced expression of Dkk3 resulted in the induction of distinct levels of reporter expression, showing the UPR initiated by the ER membrane proteins of activating transcription factor 6 (ATF6) and inositol-requring enzyme 1 (IRE1). Thus, it is possible that Dkk3 is a physiological ER stressor in the mouse adrenal gland.

    DOI: 10.18926/AMO/59950

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  • Dickkopf3 (Dkk3) is required for maintaining the integrity of secretory vesicles in the mouse adrenal medulla. 査読 国際誌

    Habuta M, Fujita H, Sato K, Bando T, Inoue J, Kondo Y, Miyaishi S, Kumon H, Ohuchi H

    Cell and tissue research   379 ( 1 )   157 - 167   2019年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/s00441-019-03113-8

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  • Localization of the ultraviolet-sensor Opn5m and its effect on myopia-related gene expression in the late-embryonic chick eye. 査読 国際誌

    Kato M, Sato K, Habuta M, Fujita H, Bando T, Morizane Y, Shiraga F, Miyaishi S, Ohuchi H

    Biochemistry and biophysics reports   19   100665 - 100665   2019年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.bbrep.2019.100665

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  • A novel photic entrainment mechanism for the circadian clock in an insect: involvement of c-fos and cryptochromes 査読

    Kutaragi Y, Tokuoka A, Tomiyama Y, Nose M, Watanabe T, Bando T, Moriyama Y, Tomioka K

    Zoological Letter   4   26   2018年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Background | Entrainment to the environmental light cycle is an essential property of the circadian clock. Although the compound eye is known to be the major photoreceptor necessary for entrainment in many insects, the molecular mechanisms of photic entrainment remain to be explored.<br />
    Results | We found that cryptochromes (crys) and c-fos mediate photic entrainment of the circadian clock in a hemimetabolous insect, the cricket Gryllus bimaculatus. We examined the effects of RNA interference (RNAi)-mediated knockdown of the cry genes, Gb’cry1 and Gb’cry2, on photic entrainment, and light-induced resetting of the circadian locomotor rhythm. Gb’cry2 RNAi accelerated entrainment for delay shifts, while Gb’cry1/ Gb’cry2 double RNAi resulted in significant lengthening of transient cycles in both advance and delay shifts, and even in entrainment failure in some crickets. Double RNAi also strongly suppressed light induced resetting. The Gb’cry-mediated phase shift or resetting of the rhythm was preceded by light-induced Gb’c-fosB expression. We also found that Gb’c-fosB, Gb’cry2 and Gb’period (Gb’per) were likely co-expressed in some optic lobe neurons.<br />
    Conclusion | Based on these results, we propose a novel model for photic entrainment of the insect circadian clock, which relies on the light information perceived by the compound eye.

    DOI: 10.1186/s40851-018-0109-8.

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  • Bone morphogenetic protein signaling in distal patterning and intercalation during leg regeneration of the cricket, Gryllus bimaculatus. 査読

    Ishimaru Y, Bando T, Ohuchi H, Noji S, Mito T

    Development, growth & differentiation   60 ( 6 )   377 - 386   2018年8月

  • Congenital eye anomalies: More mosaic than thought? 査読 国際誌

    Ohuchi H, Sato K, Habuta M, Fujita H, Bando T

    Congenital anomalies   59 ( 3 )   56 - 73   2018年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/cga.12304

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  • timeless2 plays an important role in reproduction and circadian rhythms in the cricket Gryllus bimaculatus

    Motoki Nose, Atsushi Tokuoka, Tetsuya Bando, Kenji Tomioka

    Journal of Insect Physiology   105   9 - 17   2018年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier Ltd  

    The timeless2 (tim2) gene is an insect orthologue of the mammalian clock gene Timeless (mTim). Although its functional role has been extensively studied in mammals, little is known regarding its role in insects. In the present study, we obtained tim2 cDNA (Gb'tim2) from the cricket Gryllus bimaculatus and characterized its functional role in embryonic development, egg production, and circadian rhythms. Gb'tim2 gave rise to a 1432 amino acid protein, and showed approximately 65% homology to that of Drosophila melanogaster. When treated with parental Gb'tim2RNAi, less than 2% of the treated eggs hatched. On the other hand, control eggs treated with DsRed2RNAi demonstrated a hatching rate of 70%. In most of the Gb'tim2RNAi treated embryos, development was arrested in early stages. Egg production in ovaries of adult virgin females treated with Gb'tim2RNAi was significantly reduced. In addition, while Gb'tim2RNAi crickets exhibited clear locomotor rhythm synchronized with light cycles, their light-on peak was weaker than that of control crickets. Under constant darkness, the activity rhythm of Gb'tim2RNAi crickets was often split into two components running with different periods. Molecular analysis revealed that Gb'tim2RNAi treatment downregulated mRNA levels of Gb'per and Gb'Clk, and enhanced Gb'cyc expression rhythm
    no distinct effect was found on Gb'tim expression levels. The change in Gb'per, Gb'Clk and Gb'cyc levels may underlie the altered behavioral rhythms in Gb'tim2RNAi crickets. Both Gb'ClkRNAi and Gb'cycRNAi downregulated Gb'tim2 expression, which suggested that transcription of Gb'tim2 is mediated by Gb'CLK and Gb'CYC through E-box. These results suggested that Gb'tim2 may be involved in both reproduction and circadian rhythm regulation in crickets.

    DOI: 10.1016/j.jinsphys.2017.12.007

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  • A novel photic entrainment mechanism for the circadian clock in an insect: involvement of c-fos and cryptochromes

    Yuki Kutaragi, Atsushi Tokuoka, Yasuaki Tomiyama, Motoki Nose, Takayuki Watanabe, Tetsuya Bando, Yoshiyuki Moriyama, Kenji Tomioka

    Zoological Letters   4   2018年

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  • Analysis of CRISPR genome-edited founder mice shows relationship between somatic mosaicism of Pax6 mutation and variable developmental eye abnormalities

    Akihiro Yasue, Hitomi Kono, Munenori Habuta, Tetsuya Bando, Keita Sato, Junji Inoue, Seiichi Oyadomari, Sumihare Noji, Eiji Tanaka, Hideyo Ohuchi

    MECHANISMS OF DEVELOPMENT   145   S151 - S151   2017年7月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    DOI: 10.1016/j.mod.2017.04.427

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  • cryptochrome genes form an oscillatory loop independent of the per/tim loop in the circadian clockwork of the cricket Gryllus bimaculatus

    Tokuoka, Atsushi, Itoh, Taichi Q., Hori, Shinryo, Uryu, Outa, Danbara, Yoshiki, Nose, Motoki, Bando, Tetsuya, Tanimura, Teiichi, Tomioka, Kenji

    ZOOLOGICAL LETTERS   3 ( 1 )   375 - 381   2017年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BIOMED CENTRAL LTD  

    Background: Animals exhibit circadian rhythms with a period of approximately 24 h in various physiological functions, including locomotor activity. This rhythm is controlled by an endogenous oscillatory mechanism, or circadian clock, which consists of cyclically expressed clock genes and their product proteins. cryptochrome (cry) genes are thought to be involved in the clock mechanism, and their functions have been examined extensively in holometabolous insects, but in hemimetabolous insects their role is less well understood.Results: In the present study, the role of cry genes was investigated using RNAi technology in a hemimetabolous insect, the cricket Gryllus bimaculatus. Using a molecular cloning approach, we obtained cDNAs for two cry genes: Drosophila-type cry1 (Gb ' cry1) and mammalian-type cry2 (Gb'cry2). Gb ' cry2 has six splicing variants, most of which showed rhythmic mRNA expression. Gb ' cry1(RNAi) treatment had only a limited effect at the behavioral and molecular levels, while Gb ' cry2(RNAi) had a significant effect on behavioral rhythms and molecular oscillatory machinery, alone or in combination with Gb'cry1RNAi. In Gb ' cry1/ Gb ' cry2 double-RNAi crickets, most clock genes showed arrhythmic expression, except for timeless, which retained clear rhythmic expression. Molecular analysis revealed that some combination of Gb ' cry1 and Gb ' cry2 variants suppressed CLK/CYC transcriptional activity in cultured cells.Conclusion: Based on these results, we propose a new model of the cricket's circadian clock, including a molecular oscillatory loop for Gb'cry2, which can operate independent of the Gb ' per/Gb ' tim loop.

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  • Expression analysis of Dickkopf-related protein 3 (Dkk3) suggests its pleiotropic roles for a secretory glycoprotein in adult mouse

    Junji Inoue, Hirofumi Fujita, Tetsuya Bando, Yoichi Kondo, Hiromi Kumon, Hideyo Ohuchi

    JOURNAL OF MOLECULAR HISTOLOGY   48 ( 1 )   29 - 39   2017年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Dickkopf-related protein 3 (Dkk3) is the third member of the Dkk gene family and identical to the gene, whose expression was reduced in immortalized cells. Therefore, its another name is reduced expression in immortalized cells. Since the intratumoral introduction of Dkk3 inhibits tumor growth in mouse models of cancers, Dkk3 is likely a tumor suppressor gene. However, the functions of Dkk3 in vivo remain unclear. As the first step to decipher the physiological roles of this gene, we examined the expression pattern of Dkk3 in various tissues from adult mice. In situ hybridization showed that Dkk3 mRNA was detected in the brain, retina, heart, gastrointestinal tract, adrenal glands, thymus, prostate glands, seminal vesicles, testes, and ovaries in a regionally specific manner. Furthermore, we raised anti-mouse Dkk3 antibody and performed immunohistochemistry. Cytoplasmic localization of Dkk3 protein was observed in the cells of the adrenal medulla, while Dkk3 immunoreactivity was observed in the lumen of the stomach and intestine, implying that the Dkk3 protein may be secreted into the lumen of the gastrointestinal tract. These results suggest that Dkk3 has pleiotropic roles for a secretory glycoprotein that acts primarily in the gastrointestinal tract, thymus, endocrine and reproductive organs of the mouse.

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  • Eye development and photoreception of a hemimetabolous insect, gryllus bimaculatus 査読

    Hideyo Ohuchi, Tetsuya Bando, Taro Mito, Sumihare Noji

    The Cricket as a Model Organism: Development, Regeneration, and Behavior   49 - 62   2017年1月

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    記述言語:英語   掲載種別:論文集(書籍)内論文   出版者・発行元:Springer Japan  

    The hemimetabolous insect, Gryllus bimaculatus, has two compound eyes that begin to form in the embryo and increase in size five- to sixfolds during nymphal development. Retinal stemlike cells reside in the anteroventral proliferation zone (AVPZ) of the nymphal compound eye and proliferate to increase retinal progenitors, which then differentiate to form new ommatidia in the anterior region of the eye. Here, we introduce the morphology and development of the cricket eye first, and then we focus on the roles of retinal determination genes (RDGs) such as eyes absent (eya) and sine oculis (so) in Gryllus eye formation and growth. Since the principal function of the eye is photoreception, we finally summarize opsin photopigments in this species, broadening the roles of photoreception.

    DOI: 10.1007/978-4-431-56478-2_4

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  • Relationship between somatic mosaicism of Pax6 mutation and variable developmental eye abnormalities-an analysis of CRISPR genome-edited mouse embryos

    Akihiro Yasue, Hitomi Kono, Munenori Habuta, Tetsuya Bando, Keita Sato, Junji Inoue, Seiichi Oyadomari, Sumihare Noji, Eiji Tanaka, Hideyo Ohuchi

    Scientific Reports   7 ( 1 )   1 - 13   2017年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Nature Publishing Group  

    The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-Associated protein (Cas) system is a rapid gene-Targeting technology that does not require embryonic stem cells. To demonstrate dosage effects of the Pax6 gene on eye formation, we generated Pax6-deficient mice with the CRISPR/Cas system. Eyes of founder embryos at embryonic day (E) 16.5 were examined and categorized according to macroscopic phenotype as class 1 (small eye with distinct pigmentation), class 2 (pigmentation without eye globes), or class 3 (no pigmentation and no eyes). Histologically, class 1 eyes were abnormally small in size with lens still attached to the cornea at E16.5. Class 2 eyes had no lens and distorted convoluted retinas. Class 3 eyes had only rudimentary optic vesicle-like tissues or histological anophthalmia. Genotyping of neck tissue cells from the founder embryos revealed somatic mosaicism and allelic complexity for Pax6. Relationships between eye phenotype and genotype were developed. The present results demonstrated that development of the lens from the surface ectoderm requires a higher gene dose of Pax6 than development of the retina from the optic vesicle. We further anticipate that mice with somatic mosaicism in a targeted gene generated by CRISPR/Cas-mediated genome editing will give some insights for understanding the complexity in human congenital diseases that occur in mosaic form.

    DOI: 10.1038/s41598-017-00088-w

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  • Transcriptional and nontranscriptional events are involved in photic entrainment of the circadian clock in the cricket Gryllus bimaculatus

    Yuki Kutaragi, Taiki Miki, Tetsuya Bando, Kenji Tomioka

    PHYSIOLOGICAL ENTOMOLOGY   41 ( 4 )   358 - 368   2016年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Most insects show daily activity rhythms that are controlled by endogenous circadian clocks. A basic property of the clock is entrainment to daily environmental cycles to run with an exact 24-h period. The entrainment is achieved mainly through resetting by light. The present study analyses the light resetting mechanism of the clock in first-instar nymphal and adult crickets Gryllus bimaculatus (De Geer). A 3-h light pulse given at early subjective night and late subjective night causes a phase delay and an advance, respectively, of adult locomotor rhythms. The magnitude of the shift caused by the light pulse at circadian time 12 h in constant darkness is significantly smaller than that caused by a 3-h extension of light phase. Measurement of mRNA levels during the light-induced phase shifts yielded basically similar results for nymphs and adults. When a 3-h light pulse is given at early night by light phase extension, an increase of Pdp1 occurs, which is followed by upregulation of the genes Clk and subsequently by per and tim. These changes are eliminated by RNA interference of opsin-long wavelength, which is expressed in the compound eye and encodes a green-sensitive opsin, the major photoreceptor for photic entrainment of the clock. No clear changes in mRNA levels are observed for light pulses given at late subjective night or at early subjective night after 24 h of constant darkness. These results suggest that the photic entrainment mechanism of the clock may be shared by nymphal and adult crickets, including transcriptional and nontranscriptional events.

    DOI: 10.1111/phen.12162

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  • TGF-β signaling in insects regulates metamorphosis via juvenile hormone biosynthesis

    Yoshiyasu Ishimaru, Sayuri Tomonari, Yuji Matsuoka, Takahito Watanabe, Katsuyuki Miyawaki, Tetsuya Bando, Kenji Tomioka, Hideyo Ohuchi, Sumihare Noji, Taro Mito

    Proceedings of the National Academy of Sciences of the United States of America   113 ( 20 )   5634 - 5639   2016年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1073/pnas.1600612113

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  • Autopsy Case of Bilateral Optic Nerve Aplasia with Microphthalmia: Neural Retina Formation Is Required for the Coordinated Development of Ocular Tissues

    Hideyo Ohuchi, Kaori Taniguchi, Satoru Miyaishi, Hitomi Kono, Hirofumi Fujita, Tetsuya Bando, Chiharu Fuchizawa, Yuko Ohtani, Osamu Ohtani

    ACTA MEDICA OKAYAMA   70 ( 2 )   131 - 137   2016年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    Human congenital anomalies provide information that contributes to the understanding of developmental mechanisms. Here we report bilateral optic nerve aplasia (ONA) with microphthalmia in the autopsy of the cadaver of a 70-year-old Japanese female. The gross anatomical inspection of the brain showed a cotton thread-like cord in the presumed location of the optic nerve tract or chiasm. Histologically, no neural retina, optic nerve bundle or retinal central vessels were formed in the eye globe, and the retinal pigment cells formed rosettes. The cornea, iris, and lens were also histologically abnormal. Immunohistochemically, no retinal cells expressed beta III tubulin, and Pax6-immunoreactive cells were present in the ciliary non-pigmented epithelial cells. This case of ONA could be attributed to the agenesis of retinal projection neurons as a sequel to the disruption of neural retina development. The neural retina formation would coordinate the proper development of ocular tissues.

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  • Involvement of dachshund and Distal-less in distal pattern formation of the cricket leg during regeneration

    Yoshiyasu Ishimaru, Taro Nakamura, Tetsuya Bando, Yuji Matsuoka, Hideyo Ohuchi, Sumihare Noji, Taro Mito

    SCIENTIFIC REPORTS   5   8387   2015年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Cricket nymphs have the remarkable ability to regenerate a functional leg following amputation, indicating that the regenerating blastemal cells contain information for leg morphology. However, the molecular mechanisms that underlie regeneration of leg patterns remain poorly understood. Here, we analyzed phenotypes of the tibia and tarsus (three tarsomeres) obtained by knockdown with regeneration-dependent RNA interference (rdRNAi) against Gryllus dachshund (Gb'dac) and Distal-less (Gb'Dll). We found that depletion of Gb'Dll mRNA results in loss of the tarsal segments, while rdRNAi against Gb'dac shortens the tibia at the two most distal tarsomeres. These results indicate that Gb'Dll expression is indispensable for formation of the tarsus, while Gb'dac expression is necessary for elongation of the tibia and formation of the most proximal tarsomere. These findings demonstrate that mutual transcriptional regulation between the two is indispensable for formation of the tarsomeres, whereas Gb'dac is involved in determination of tibial size through interaction with Gb'ds/Gb'ft.

    DOI: 10.1038/srep08387

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  • An extended steepness model for leg-size determination based on Dachsous/Fat trans- dimer system

    Hiroshi Yoshida, Tetsuya Bando, Taro Mito, Hideyo Ohuchi, Sumihare Noji

    SCIENTIFIC REPORTS   4   4335   2014年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    What determines organ size has been a long-standing biological question. Lawrence et al. (2008) proposed the steepness hypothesis suggesting that the protocadherin Dachsous/Fat (Ds/Ft) system may provide some measure of dimension to the cells in relation to the gradient. In this paper we extended the model as a means of interpreting experimental results in cricket leg regeneration. We assumed that (1) Ds/Ft trans-heterodimers or trans-homodimers are redistributed during cell division, and (2) growth would cease when a differential of the dimer across each cell decreases to a certain threshold. We applied our model to simulate the results obtained by leg regeneration experiments in a cricket model. The results were qualitatively consistent with the experimental data obtained for cricket legs by RNA interference methodology. Using our extended steepness model, we provided a molecular-based explanation for leg size determination even in intercalary regeneration and for organ size determination.

    DOI: 10.1038/srep04335

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  • The expression of LIM-homeobox genes, Lhx1 and Lhx5, in the forebrain is essential for neural retina differentiation

    Junji Inoue, Yuuki Ueda, Tetsuya Bando, Taro Mito, Sumihare Noji, Hideyo Ohuchi

    DEVELOPMENT GROWTH & DIFFERENTIATION   55 ( 7 )   668 - 675   2013年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Elucidating the mechanisms underlying eye development is essential for advancing the medical treatment of eye-related disorders. The primordium of the eye is an optic vesicle (OV), which has a dual potential for generation of the developing neural retina and retinal pigment epithelium. However, the factors that regulate the differentiation of the retinal primordium remain unclear. We have previously shown that overexpression of Lhx1 and Lhx5, members of the LIM-homeobox genes, induced the formation of a second neural retina from the presumptive pigmented retina of the OV. However, the precise timing of Lhx1 expression required for neural retina differentiation has not been clarified. Moreover, RNA interference of Lhx5 has not been previously reported. Here, using a modified electroporation method, we show that, Lhx1 expression in the forebrain around stage 8 is required for neural retina formation. In addition, we have succeeded in the knockdown of Lhx5 expression, resulting in conversion of the neural retina region to a pigment vesicle-like tissue, which indicates that Lhx5 is also required for neural retina differentiation, which correlates temporally with the activity of Lhx1. These results suggest that Lhx1 and Lhx5 in the forebrain regulate neural retina differentiation by suppressing the development of the retinal pigment epithelium, before the formation of the OV.

    DOI: 10.1111/dgd.12074

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  • Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases

    Takahito Watanabe, Hiroshi Ochiai, Tetsushi Sakuma, Hadley W. Horch, Naoya Hamaguchi, Taro Nakamura, Tetsuya Bando, Hideyo Ohuchi, Takashi Yamamoto, Sumihare Noji, Taro Mito

    NATURE COMMUNICATIONS   3   2012年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Hemimetabolous, or incompletely metamorphosing, insects are phylogenetically relatively basal and comprise many pests. However, the absence of a sophisticated genetic model system, or targeted gene-manipulation system, has limited research on hemimetabolous species. Here we use zinc-finger nuclease and transcription activator-like effector nuclease technologies to produce genetic knockouts in the hemimetabolous insect Gryllus bimaculatus. Following the microinjection of mRNAs encoding zinc-finger nucleases or transcription activator-like effector nucleases into cricket embryos, targeting of a transgene or endogenous gene results in sequence-specific mutations. Up to 48% of founder animals transmit disrupted gene alleles after zinc-finger nucleases microinjection compared with 17% after microinjection of transcription activator-like effector nucleases. Heterozygous offspring is selected using mutation detection assays that use a Surveyor (Cel-I) nuclease, and subsequent sibling crosses create homozygous knockout crickets. This approach is independent from a mutant phenotype or the genetic tractability of the organism of interest and can potentially be applied to manage insect pests using a non-transgenic strategy.

    DOI: 10.1038/ncomms2020

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  • Expression profiles and unc-27 mutation rescue of the striated muscle type troponin I isoform-3 in Caenorhabditis elegans

    Yasuo Takashima, Shun Kitaoka, Tetsuya Bando, Hiroaki Kagawa

    GENES & GENETIC SYSTEMS   87 ( 4 )   243 - 251   2012年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:GENETICS SOC JAPAN  

    Transcription control of multiple genes in tissue- and stage-specific patterns is still of major interest. We show here that troponin I (TNT) is expressed under the control of upstream non-coding sequences and had functions as an isoform of intermediate type between pharynx and body-wall of the gene. In Caenorhabditis elegans, three striated muscle TNIs are expressed in body-wall muscles and a cardiac isoform is expressed in the pharynx. We have analyzed the gene expression mechanisms of tni-3 gene and motility function of its protein product. Promoter deletion analysis of the tni-3 gene identified muscle enhancers including the head enhancer. The CBF1/Su(H)/LAG-1-binding motif was included in the head enhancer. Yeast one-hybrid screening isolated the lag-1 clone in five candidates. Functional differences between the three striated muscle TNIs were investigated by the expression of promoter-fusion genes into tni-2/unc-27(e155) null mutant animals. The results suggest that the cis-elements in the promoters of the three genes are important for their tissue-specific expression and that from the function of TNI-3, the tni-3 gene would be an intermediate in the evolution of these genes by gene duplication. Mechanisms of tni-3 expression and its molecular function may contribute to our understanding of gene evolution and developmental programs.

    DOI: 10.1266/ggs.87.243

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  • Functional analysis of the role of eyes absent and sine oculis in the developing eye of the cricket Gryllus bimaculatus

    Akira Takagi, Kazuki Kurita, Taiki Terasawa, Taro Nakamura, Tetsuya Bando, Yoshiyuki Moriyama, Taro Mito, Sumihare Noji, Hideyo Ohuchi

    DEVELOPMENT GROWTH & DIFFERENTIATION   54 ( 2 )   227 - 240   2012年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    In the cricket Gryllus bimaculatus, a hemimetabolous insect, the compound eyes begin to form in the embryo and increase 56 fold in size during the postembryonic development of the nymphal stage. Retinal stem cells in the anteroventral proliferation zone (AVPZ) of the nymphal eye proliferate to increase retinal progenitors, which then differentiate to form new ommatidia in the anterior region of the eye. However, mechanisms underlying this type of eye formation have not been well elucidated yet. Here, we found that the homologues of the retinal determination transcription factor genes of eyes absent (eya) and sine oculis (so) are expressed during the cricket embryonic eye formation. eya is also expressed intensely in the AVPZ of the nymphal eye. To explore their functions, we performed knockdown by RNA interference (RNAi). Knockdown of Gbeya resulted in loss of the embryonic eye. In the nymphal eye, RNAi against Gbeya or Gbso impaired retinal morphology by apparently transforming cornea structures into head cuticle. These results imply that Gbeya and Gbso are essential for the differentiation of the retinal progenitor cells and maintaining retinal structures during eye development.

    DOI: 10.1111/j.1440-169X.2011.01325.x

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  • Cricket body size is altered by systemic RNAi against insulin signaling components and epidermal growth factor receptor 査読

    Noha Dabour, Tetsuya Bando, Taro Nakamura, Katsuyuki Miyawaki, Taro Mito, Hideyo Ohuchi, Sumihare Noji

    DEVELOPMENT GROWTH & DIFFERENTIATION   53 ( 7 )   857 - 869   2011年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    A long-standing problem of developmental biology is how body size is determined. In Drosophila melanogaster, the insulin/insulin-like growth factor (I/IGF) and target of rapamycin (TOR) signaling pathways play important roles in this process. However, the detailed mechanisms by which insect body growth is regulated are not known. Therefore, we have attempted to utilize systemic nymphal RNA interference (nyRNAi) to knockdown expression of insulin signaling components including Insulin receptor (InR), Insulin receptor substrate (chico), Phosphatase and tensin homologue (Pten), Target of rapamycin (Tor), RPS6-p70-protein kinase (S6k), Forkhead box O (FoxO) and Epidermal growth factor receptor (Egfr) and observed the effects on body size in the Gryllus bimaculatus cricket. We found that crickets treated with double-stranded RNA (dsRNA) against Gryllus InR, chico, Tor, S6k and Egfr displayed smaller body sizes, while Gryllus FoxO nyRNAi-ed crickets exhibited larger than normal body sizes. Furthermore, RNAi against Gryllus chico and Tor displayed slow growth and RNAi against Gryllus chico displayed longer lifespan than control crickets. Since no significant difference in ability of food uptake was observed between the Gryllus chico(nyRNAi) nymphs and controls, we conclude that the adult cricket body size can be altered by knockdown of expressions of Gryllus InR, chico, Tor, S6k, FoxO and Egfr by systemic RNAi. Our results suggest that the cricket is a promising model to study mechanisms underlying controls of body size and life span with RNAi methods.

    DOI: 10.1111/j.1440-169X.2011.01291.x

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  • Regulation of Leg Size and Shape: Involvement of the Dachsous-Fat Signaling Pathway 査読

    Tetsuya Bando, Taro Mito, Taro Nakamura, Hideyo Ohuchi, Sumihare Noji

    DEVELOPMENTAL DYNAMICS   240 ( 5 )   1028 - 1041   2011年5月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    How limb size and shape is regulated is a long-standing question in developmental and regeneration biology. Recently, the protocadherin Dachsous-Fat (Ds-Ft) signaling pathway has been found to be essential for wing development of the fly and leg regeneration of the cricket. The Ds-Ft signaling pathway is linked to the Warts-Hippo (Wts-Hpo) signaling pathway, leading to cell proliferation. Several lines of evidence have suggested that the Wts-Hpo signaling pathway is involved in the control of organ size, and that this pathway is regulated by Ds-Ft and Merlin-Expanded, which are linked to morphogens such as decapentaplegic/bone morphogenic protein, Wingless/Wnt, and epidermal growth factor. Here we review recent progress in elucidating mechanisms controlling leg size and shape in insects and vertebrates, focusing on the Ds-Ft signaling pathway. We also introduce a working model, Ds-Ft steepness model, to explain how steepness of the Ds-Ft gradient controls leg size along the proximodistal axis. Developmental Dynamics 240:1028-1041, 2011. (C) 2011 Wiley-Liss, Inc.

    DOI: 10.1002/dvdy.22590

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  • The advent of RNA interference in Entomology

    Taro Mito, Taro Nakamura, Tetsuya Bando, Hideyo Ohuchi, Sumihare Noji

    ENTOMOLOGICAL SCIENCE   14 ( 1 )   1 - 8   2011年1月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    RNA interference (RNAi) is a cellular process by which an mRNA is targeted for degradation by a small interfering RNA that contains a strand complementary to a fragment of the target mRNA, resulting in sequence specific inhibition of gene expression. The discovery of RNAi enabled the use of loss-of-function analyses in many non-model insects other than Drosophila to elucidate the roles of specific genes. The RNAi approach has been widely used on insects in several fields, including embryogenesis, pattern formation, reproduction, biosynthesis and behavior. The increasing availability of insect genomes has made the RNAi technique an indispensable technique for characterizing gene functions in insects. Here we review the current status of RNAi-based experiments in insects and the applications of RNAi for species-specific insecticides, focusing on non-drosophilid insects. We also identify future applications for RNAi-based studies in Entomology.

    DOI: 10.1111/j.1479-8298.2010.00408.x

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  • Imaging of Transgenic Cricket Embryos Reveals Cell Movements Consistent with a Syncytial Patterning Mechanism 査読

    Taro Nakamura, Masato Yoshizaki, Shotaro Ogawa, Haruko Okamoto, Yohei Shinmyo, Tetsuya Bando, Hideyo Ohuchi, Sumihare Noji, Taro Mito

    CURRENT BIOLOGY   20 ( 18 )   1641 - 1647   2010年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CELL PRESS  

    The mode of insect embryogenesis varies among species, reflecting adaptations to different lite history strategies [1, 2]. In holometabolous insects, which include the model systems, such as the fruit fly and the red flour beetle, a large proportion of the blastoderm produces an embryo, whereas hemimetabolous embryos generally arise from a small region of the blastoderm [3]. Despite their importance in evolutionary studies, information of early developmental dynamics of hemimetabolous insects remains limited. Here, to clarify how maternal and gap gene products act in patterning the embryo of basal hemimetabolous insects, we analyzed the dynamic segmentation process in transgenic embryos of an intermediate-germ insect species, the cricket, Gryllus bimaculatus. Our data based on live imaging of fluorescently labeled embryonic cells and nuclei suggest that the positional specification of the cellular blastoderm may be established in the syncytiurn, where maternally derived gradients could act fundamentally in a way that is similar to that of Drosophila, namely throughout the egg. Then, the blastoderm cells move dynamically, retaining their positional information to form the posteriorly localized germ anlage. Furthermore, we find that the anterior head region of the cricket embryo is specified by orthodenticle in a cellular environment earlier than the gnathal and thoracic regions. Our findings imply that the syncytial mode of the early segmentation in long-germ insects evolved from a dynamic syncytial-to-cellular mode found in the present study, accompanied by a heterochronic shift of gap gene action.

    DOI: 10.1016/j.cub.2010.07.044

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  • Involvement of the Hedgehog/Engrailed signaling in regeneration of the posterior structures in the amputated leg of the cricket Gryllus bimaculatus

    Katsuyuki Miyawaki, Taro Mito, Taro Nakamura, Tetsuya Bando, Kimio Tanaka, Hideyo Ohuchi, Sumihare Noji

    MECHANISMS OF DEVELOPMENT   126   S294 - S294   2009年8月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    DOI: 10.1016/j.mod.2009.06.799

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  • Regulation of leg size and shape by the Dachsous/Fat signalling pathway during regeneration in Gryllus bimaculatus

    Tetsuya Bando, Taro Mito, Yuko Maeda, Taro Nakamura, Fumiaki Ito, Takahito Watanabe, Hideyo Ohuchi, Sumihare Noji

    MECHANISMS OF DEVELOPMENT   126   S293 - S294   2009年8月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    DOI: 10.1016/j.mod.2009.06.797

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  • Dynamics of cellular aggregation and differentiation during early development of transgenic crickets

    Taro Nakamura, Taro Mito, Masato Yoshizaki, Tetsuya Bando, Kimio Tanaka, Hideyo Ohuchi, Sumihare Noji

    MECHANISMS OF DEVELOPMENT   126   S254 - S254   2009年8月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    DOI: 10.1016/j.mod.2009.06.666

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  • Tissue-specific interactions of TNI isoforms with other TN subunits and tropomyosins in C. elegans: the role of the C- and N-terminal extensions. 査読

    Amin MZ, Bando T, Ruksana R, Anokye-Danso F, Takashima Y, Sakube Y, Kagawa H

    Biochim Biophys Acta   1774   456 - 465   2007年4月

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  • A novel non-coding DNA family in Caenorhabditis elegans 査読

    Yasuo Takashima, Tetsuya Bando, Hiroaki Kagawa

    GENE   388 ( 1-2 )   61 - 73   2007年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Many repetitive elements, for example, SINEs, LINEs, LTR-retrotransposons and other SSRs are dispersed throughout eukaryotic genomes. To understand the biological function of these repetitive elements is of great current research interest. In this study, we report on the identification of a novel non-coding DNA family, designated CE1 family, in the nematode C. elegans genome. Some CE1 elements constituted a large palindrome sequence. The CE1 elements were interspersed at 95 sites in the C. elegans genome. Most of the CE1 elements were associated with, or were within, protein-coding genes. The sequence of the CE1 elements indicated that some could form a hairpin structure. One of the CE1 family, CE1 (bs258), is located in the first intron of a novel gene, C46H11.6 which encodes a PDZ/DHR/GLGF domain protein. In gfp and lacZ reporter gene assays the CE1 (bs258) element appeared to behave as an enhancer element for the expression of C46H11.6 but no effect on the expression of the opposite direction gene, pat-10 which encodes the body-wall muscle troponin C. The CE1 (bs258) RNA transcript was detected by RT-PCR even when CE1(bs258) was located in an intron. We conclude that CE1 elements are involved in the expression of adjacent genes and are therefore selectively retained in the C. elegans genome. We discussed a biological function of the CE1(bs258) having many transcription factor-binding sites. (c) 2006 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.gene.2006.10.002

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  • Involvement of canonical Wnt/Wingless signaling in the determination of the positional values within the leg segment of the cricket Gryllus bimaculatus 査読

    Taro Nakamura, Taro Mito, Yoshihisa Tanaka, Tetsuya Bando, Hideyo Ohuchi, Sumihare Noji

    DEVELOPMENT GROWTH & DIFFERENTIATION   49 ( 2 )   79 - 88   2007年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING  

    The cricket Gryllus bimaculatus is a hemimetabolous insect whose nymphs posses the ability to regenerate amputated legs. Previously, we showed that Gryllus orthologues of Drosophila hedgehog (Gb'hh), wingless (Gb'wg) and decapentaplegic (Gb'dpp) are expressed during leg regeneration and play essential roles in the establishment of the proximal-distal axis. Here, we examined their roles during intercalary regeneration: when a distally amputated tibia with disparate positional values is placed next to a proximally amputated host, intercalary growth occurs in order to regenerate the missing part. In this process, we examined expression patterns of Gb'hh and Gb'wg. We found that expressions of Gb'hh and Gb'wg were induced in a regenerate and the host proximal to the amputated region, but not in the grafted donor distal to the regenerate. This directional induction occurs even in the reversed intercalation. Because these results are consistent with a distal-to-proximal respecification of the regenerate, Gb'wg may be involved in the re-establishment of the positional values in the regenerate. Furthermore, we found that no regeneration occurs when Gb'armadillo (the orthologue of beta-catenin) was knocked down by RNA interference. These results indicate that the canonical Wnt/Wingless signaling pathway is involved in the process of leg regeneration and determination of positional information in the leg segment.

    DOI: 10.1111/j.1440-169x.2007.00915.x

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  • Tissue expression of four troponin I genes and their molecular interactions with two troponin C isoforms in Caenorhabditis elegans. 査読

    Ruksana R, Kuroda K, Terami H, Bando T, Kitaoka S, Takaya T, Sakube Y, Kagawa H

    Genes Cells   10 ( 3 )   261-276 - 276   2005年

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書籍等出版物

  • 最先端コオロギ学 : 世界初!新しい生物学がここにある

    野地, 澄晴( 担当: 分担執筆 ,  範囲: 第6章 コオロギの切断された脚の再生メカニズム)

    北隆館  2022年4月  ( ISBN:9784832610125

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    総ページ数:vi, 242p   記述言語:日本語

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  • The Cricket as a Model Organism: Development, Regeneration, and Behavior.

    Bando, T, Hamada, Y, Noji, S( 担当: 分担執筆 ,  範囲: Leg formation and regeneration)

    Springer Japan  2017年1月 

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  • The Cricket as a Model Organism: Development, Regeneration, and Behavior.

    Ohuchi, H, Bando, T, Mito, T, Noji, S( 担当: 分担執筆 ,  範囲: Eye development and photoreception of a hemimetabolous insect, gryllus bimaculatus)

    Springer Japan  2017年1月 

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  • クロマチンと遺伝子機能制御

    板東哲哉, 香川弘昭( 担当: 分担執筆 ,  範囲: 線虫のクロマチンと遺伝子制御)

    シュプリンガー・フェアラーク東京  2003年6月 

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MISC

  • フタホシコオロギが解き明かす免疫と再生の思いがけない関係

    板東 哲哉, 奥村 美紗, 濱田 良真, 大内 淑代

    昆虫と自然   57 ( 1 )   33 - 36   2021年12月

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    担当区分:筆頭著者   記述言語:日本語  

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  • 昆虫に学ぶ器官再生

    板東哲哉, 濱田良真, 奥村美紗, 坂東優希, 大内淑代

    ケミカルタイムズ   241 ( 3 )   2 - 6   2016年

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  • コオロギ脚再生の分子メカニズム

    実験医学   32 ( 1 )   15 - 21   2014年

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  • ゲノムの地理と歴史―非コード領域の生物学的機能― 査読

    香川弘昭, 板東哲哉, 高島康郎

    生物物理   49 ( 5 )   246 - 248   2009年

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    記述言語:日本語   出版者・発行元:The Biophysical Society of Japan General Incorporated Association  

    DOI: 10.2142/biophys.49.246

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  • コオロギ脚再生におけるNADPH oxidasesにより産生される活性酸素の役割

    奥村美紗, 板東哲哉, 大内淑代

    日本分子生物学会年会プログラム・要旨集(Web)   45th   2022年

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  • Fgf10ゲノム編集モザイク変異マウスの肺と四肢の表現型解析

    土生田宗憲, 泰江章博, 鈴木賢一, 鈴木賢一, 藤田洋史, 佐藤恵太, 河野仁美, 高山鮎子, 板東哲哉, 宮石智, 親泊政一, 田中栄二, 大内淑代

    日本分子生物学会年会プログラム・要旨集(Web)   43rd   2020年

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  • Toll受容体によるコオロギ脚再生の促進メカニズム

    奥村美紗, 板東哲哉, 坂東優希, 萩原万優, 大内淑代

    日本分子生物学会年会プログラム・要旨集(Web)   42nd   2019年

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  • 紫外線受容視物質オプシン5のニワトリ胚線維芽細胞における作用

    加藤睦子, 加藤睦子, 佐藤恵太, 藤田洋史, 板東哲哉, 森實祐基, 中山正, 白神史雄, 大内淑代

    日本眼科学会雑誌   122   2018年

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  • Molecular and cellular basis of regeneration and tissue repair: Dissecting insect leg regeneration through RNA interference

    T. Nakamura, T. Mito, T. Bando, H. Ohuchi, S. Noji

    Cellular and Molecular Life Sciences   65 ( 1 )   64 - 72   2008年1月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等  

    Nymphs of hemimetabolous insects such as cockroaches and crickets exhibit a remarkable capacity for regenerating complex structures from damaged legs. Until recent years, however, approaches to elucidate the molecular mechanisms underlying the leg regeneration process have been lacking. Taking the cricket Gryllus bimaculatus as a model, we found that a phenotype related to regeneration frequently appears during leg regeneration, even though no phenotype is induced by RNA interference (RNAi) in the cricket nymph, designated as regeneration-dependent RNAi. Since then, we have investigated the functions of various genes encoding signaling factors and cellular adhesion proteins like Fat and Dachsous during leg regeneration. In this review, we summarize the classical knowledge about insect leg regeneration and introduce recent advances concerning the signaling cascades required for regenerating a leg. Our results provide clues to the mechanisms of regeneration which are relevant to vertebrate systems. © 2007 Birkhaueser.

    DOI: 10.1007/s00018-007-7432-0

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  • RNA干渉を用いたコオロギの遺伝子機能解析法の研究

    中村 太郎, 濱田 明日香, 板東 哲哉, 三戸 太郎, 大内 淑代, 野地 澄晴

    電子情報通信学会技術研究報告. MBE, MEとバイオサイバネティックス   107 ( 154 )   91 - 93   2007年7月

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    記述言語:日本語   出版者・発行元:一般社団法人電子情報通信学会  

    コオロギは、胚発生や脚形成の様式がショウジョウバエと異なる。また、コオロギ幼虫の脚は切断されても再生可能であることが知られている。コオロギの胚発生と脚再生過程に働く遺伝子の機能を解析するために、我々はRNAi法を用いている。これまでに、 embryonic, parentalそして、nymphal RNAi法を確立し、次の事を明らかにした。コオロギにおいては、(1)ショウジョウバエ(Bicoid)と異なる因子(Caudal)により、胚の前後の位置情報が与えられている可能性がある、(2)winglessシグナル伝達経路が再生過程で働いておりそして、再生過程における遠近軸に沿った位置情報の決定に関与している、(3)ヒト脆弱X症侯群の原因遺伝子のコオロギホモログfmrは、コオロギの鳴き方に影響を与える遺伝子である、(4)diphenol oxidaseをコードするlaccase 2はコオロギ外骨格の着色、硬化に関与している。

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  • Tissue expression of the tropomyosin gene of Caenorhabditis elegans--enhancer modification of the promoter control

    Hiroaki Kagawa, Tetsuya Bando

    Seikagaku. The Journal of Japanese Biochemical Society   74 ( 9 )   1176 - 1180   2002年

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    記述言語:日本語   掲載種別:書評論文,書評,文献紹介等  

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講演・口頭発表等

  • フタホシコオロギの脚再生と自然免疫 招待

    板東哲哉, 奥村美紗, 大内淑代

    基礎生物学研究所 共同利用研究課題 研究会 Looking back of studies on planarian regeneration and future directions  2024年8月21日 

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    開催年月日: 2024年8月20日 - 2024年8月21日

    記述言語:日本語   会議種別:口頭発表(招待・特別)  

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  • Regulation of blastema cell proliferation during cricket leg regeneration via reactive oxygen species

    Misa Okumura, Tetsuya Bando, Hideyo Ohuchi

    The 56th annual meeting of the Japanese Society of Developmental Biologists  2023年7月23日 

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    開催年月日: 2023年7月22日 - 2023年7月25日

    記述言語:英語   会議種別:ポスター発表  

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  • コオロギ脚再生におけるNADPH oxidasesにより産生される活性酸素の役割

    奥村 美紗, 板東 哲哉, 大内 淑代

    第45回 分子生物学会年会  2022年11月30日 

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    開催年月日: 2022年11月30日 - 2022年12月2日

    会議種別:ポスター発表  

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  • マクロファージと活性酸素による昆虫脚再生の制御

    板東哲哉、奥村美紗、大内淑代

    日本解剖学会 第 76 回 中国・四国支部学術集会  2022年10月30日 

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    開催年月日: 2022年10月29日 - 2022年10月30日

    会議種別:口頭発表(一般)  

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  • NADPH oxidasesによるコオロギ脚再生の制御メカニズム

    板東哲哉, 奥村美紗, 大内淑代

    第44回日本分子生物学会年会  2021年12月2日 

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    開催年月日: 2021年12月1日 - 2021年12月3日

    会議種別:ポスター発表  

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  • 昆虫脚再生におけるToll様受容体とCD36による再生芽細胞の増殖の制御

    板東哲哉, 奥村美紗, 大内淑代

    第126回日本解剖学会総会・全国学術集会 合同大会 第126回 日本解剖学会総会・全国学術集会 第98回 日本生理学会大会 合同大会  2021年3月29日 

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    開催年月日: 2021年3月28日 - 2021年3月30日

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  • Toll様受容体とスカベンジャー受容体CD36による昆虫の器官再生メカニズム

    板東哲哉, 奥村美紗, 坂東優希, 萩原万優, 大内淑代

    第125回日本解剖学会総会・全国学術集会  2020年3月26日 

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    開催年月日: 2020年3月25日 - 2020年3月27日

    会議種別:ポスター発表  

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  • Fgf10モザイク変異体における肺表現型および下肢帯表現型の形態学的解析

    土生田宗憲, 泰江章博, 鈴木賢一, 高山鮎子, 藤田洋史, 板東哲哉, 佐藤恵太, 親泊政一, 田中栄二, 大内淑代

    日本先天異常学会学術集会プログラム・抄録集  2020年 

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    開催年月日: 2020年

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  • ゲノム編集Fgf10モザイク変異体の遺伝子型と表現型解析

    土生田宗憲, 泰江章博, 鈴木賢一, 藤田洋史, 高山鮎子, 佐藤恵太, 板東哲哉, 親泊政一, 田中栄二, 大内淑代

    日本解剖学会総会・全国学術集会講演プログラム・抄録集  2020年 

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    開催年月日: 2020年

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  • Toll受容体によるコオロギ脚再生の促進メカニズム

    奥村美紗, 板東哲哉, 坂東優希, 萩原万優, 大内淑代

    第42回日本分子生物学会年会  2019年12月3日 

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    開催年月日: 2019年12月3日 - 2019年12月6日

    会議種別:ポスター発表  

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  • 神経網膜形成における転写因子Lhx1の下流遺伝子の機能解析

    衣畑俊希, 佐藤恵太, 藤田洋史, 板東哲哉, 濃野勉, 大内淑代

    日本分子生物学会年会プログラム・要旨集(Web)  2019年 

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    開催年月日: 2019年

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  • REIC/Dickkopf3(Dkk3)の副腎における機能解析

    土生田宗憲, 藤田洋史, 佐藤恵太, 板東哲哉, 公文裕巳, 大内淑代

    日本解剖学会総会・全国学術集会講演プログラム・抄録集  2019年 

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    開催年月日: 2019年

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  • 極性分子複合体はコオロギ脚再生に必要である

    板東 哲哉, 濱田 良真, 三戸 太郎, 野地 澄晴, 大内 淑代

    第41回日本分子生物学会年会  2018年11月29日 

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    開催年月日: 2018年11月28日 - 2018年11月30日

    会議種別:ポスター発表  

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  • マクロファージによる器官再生の促進:昆虫の脚再生をモデルとして

    板東哲哉, 奥村美紗, 坂東優希, 萩原万優子, 濱田良真, 大内淑代

    日本解剖学会 第73回中国・四国支部学術集会  2018年10月20日 

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    開催年月日: 2018年10月20日 - 2018年10月21日

    会議種別:口頭発表(一般)  

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  • ゲノム編集Fgf10モザイク変異体の組織学的解析

    土生田宗憲, 泰江章博, 藤田洋史, 板東哲哉, 佐藤恵太, 親泊政一, 田中栄二, 大内淑代

    日本解剖学会総会・全国学術集会講演プログラム・抄録集  2018年 

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    開催年月日: 2018年

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  • Fgf10モザイク変異体における肺表現型の形態学的解析

    土生田宗憲, 泰江章博, 藤田洋史, 板東哲哉, 佐藤恵太, 親泊政一, 田中栄二, 大内淑代

    日本先天異常学会学術集会プログラム・抄録集  2018年 

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    開催年月日: 2018年

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  • Lhx1過剰発現による二重網膜誘導モデルを用いた網膜初期発生の研究

    衣畑俊希, 佐藤恵太, 藤田洋史, 板東哲哉, 大内淑代

    日本分子生物学会年会プログラム・要旨集(Web)  2018年 

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    開催年月日: 2018年

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  • CRISPR/Cas9システムにより作製したモザイク変異マウスの組織学的解析

    土生田宗憲, 泰江章博, 藤田洋史, 板東哲哉, 佐藤恵太, 親泊政一, 田中栄二, 大内淑代

    日本生化学会大会(Web)  2017年 

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    開催年月日: 2017年

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  • Fat and dachsous protocadherin are required for proper proximodistal and circumferential regeneration of amputated legs in the cricket, Gryllus bimaculatus

    Tetsuya Bando, Yuko Maeda, Taro Nakamura, Taro Mito, Hideyo Ohuchi, Sumihare Noji

    GENES & GENETIC SYSTEMS  2007年12月  GENETICS SOC JAPAN

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    開催年月日: 2007年12月

    記述言語:英語  

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  • Warts, Expanded and Dachs are required for proper regeneration of amputated legs in the cricket, Gryllus bimaculatus

    Yuko Maeda, Tetsuya Bando, Taro Nakamura, Taro Mito, Hideyo Ohuchi, Sumihare Noji

    GENES & GENETIC SYSTEMS  2007年12月  GENETICS SOC JAPAN

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    開催年月日: 2007年12月

    記述言語:英語  

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  • Innate immunity signaling pathways promote leg regeneration in the cricket

    第70回日本細胞生物学会 第51回日本発生生物学会 合同大会  2018年 

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  • 再生モデル昆虫ではToll様受容体が器官再生を促進する

    第123回 日本解剖学会総会・全国学術集会  2018年 

     詳細を見る

  • 再生モデル昆虫における自然免疫シグナル経路を介した器官再生の促進メカニズム

    ConBio2017  2017年 

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  • 再生モデル昆虫におけるマクロファージを介した再生開始メカニズム

    第122回 日本解剖学会総会・全国学術集会  2017年 

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  • がん関連遺伝子 Dickkopf-3(Dkk-3)の生体内における発現パターンの解析

    第57回日本組織細胞化学会総会・学術集会  2016年 

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  • 再生モデル昆虫における器官再生のメカニズム

    第121回 日本解剖学会総会・全国学術集会  2016年 

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  • フタホシコオロギ脚再生におけるマクロファージを中心とした炎症反応の役割

    第2回 ユニークな少数派実験動物を扱う若手が最先端アプローチを勉強する会  2016年 

     詳細を見る

  • Molecular link between innate immunity and tissue regeneration

    Cricket Meeting 2016  2016年 

     詳細を見る

  • Angiomotin regulates cell proliferation cooperatively with Expanded and Merlin during cricket leg regeneration

    第38回 日本分子生物学会年会  2015年 

     詳細を見る

  • Cyclin B3 is involved in leg regeneration of the cricket Gryllus bimaculatus

    第120回 日本解剖学会総会・全国学術集会  2015年 

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  • Epigenetic regulation on histone H3K27 is involved in redifferentiation process during leg regeneration in the cricket Gryllus bimaculatus

    第120回 日本解剖学会総会・全国学術集会  2015年 

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  • ポリプテルス眼の発生分化と機能に関わる遺伝子群

    第5回 Tokyo Vertebrate Morphology Meeting  2015年 

     詳細を見る

  • 再生モデル昆虫コオロギに学ぶ器官再生のメカニズム

    再生生物学シンポジウム『次世代型器官再生生物学の発展』  2015年 

     詳細を見る

  • コオロギ脚再生において再生芽細胞の再分化はヒストンH3K27メチル化により制御される

    第37回 日本分子生物学会年会  2014年 

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  • CRISPR/CasシステムによるPax6遺伝子破壊マウスの解析

    第37回 日本分子生物学会年会  2014年 

     詳細を見る

  • コオロギにおけるエピジェネティック因子E(z)の概日リズムおよび再生への関与

    日本動物学会 第85回大会  2014年 

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  • コオロギに学ぶ器官再生の分子メカニズム

    第119回 日本解剖学会総会・全国学術集会  2014年 

     詳細を見る

  • Molecular basis on tissue regeneration in the cricket as revealed by RNA interference

    International Symposium on RNAi and Genome Editing Research  2014年 

     詳細を見る

  • Mechanisms underlying regeneration of the cricket leg, based on an extended steepness model

    EMBO Conference The Molecular and cellular basis of regeneration and tissue repair  2014年 

     詳細を見る

  • Epigenetic regulation of genes expressions via methylation on histone H3 27th lysine residue during leg regeneration

    第47回 日本発生生物学会  2014年 

     詳細を見る

  • Epigenetic regulation of genes expressions via methylation on histone H3K27 during leg regeneration in the cricket Gryllus bimaculatus

    CDBシンポジウム2014 Regeneration of Organs: Programming and Self-Organization  2014年 

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  • 再生モデル昆虫を用いた器官再生の分子基盤の研究

    第12回日本再生医療学会総会  2013年 

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  • 再生を制御する普遍的分子機構の解明:昆虫の脚再生からヒト器官再生を目指して

    日本解剖学会 第68回中国・四国支部学術集会  2013年 

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  • フタホシコオロギ脚再生過程におけるパターン形成遺伝子のエピジェネティックな発現制御

    日本動物学会第84回大会  2013年 

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  • 器官再生において再生芽細胞の再分化を制御するエピジェネティックな機構

    日本分子生物学会第36回年会  2013年 

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  • JAK/STAT signaling promotes blastemal cell proliferation during leg regeneration in the cricket Gryllus bimaculatus

    第46回日本発生生物学会  2013年 

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  • コオロギ脚再生において再生芽細胞の増殖を制御する分子メカニズムの解析

    第35回日本分子生物学会年会  2012年 

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  • Molecular mechanism for regulation of blastema cell proliferation during leg regeneration in Gryllus bimaculatus

    2012年 

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▼全件表示

共同研究・競争的資金等の研究

  • Toll様受容体シグナルが促進する脱分化誘導因子のエピジェネティックな発現制御

    研究課題/領域番号:21K06121  2021年04月 - 2024年03月

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    板東 哲哉

      詳細を見る

    担当区分:研究代表者  資金種別:競争的資金

    配分額:4160000円 ( 直接経費:3200000円 、 間接経費:960000円 )

  • マクロファージにより活性化される脱分化誘導因子のエピジェネティックな発現制御

    研究課題/領域番号:18K06184  2018年04月 - 2022年03月

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    板東 哲哉

      詳細を見る

    担当区分:研究代表者  資金種別:競争的資金

    配分額:4290000円 ( 直接経費:3300000円 、 間接経費:990000円 )

  • 器官再生において再生芽細胞への脱分化を誘導するエピジェネティックな機構

    研究課題/領域番号:15K06897  2015年04月 - 2018年03月

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    板東 哲哉

      詳細を見る

    担当区分:研究代表者  資金種別:競争的資金

    配分額:5070000円 ( 直接経費:3900000円 、 間接経費:1170000円 )

  • 器官再生において再生芽細胞の脱分化と再分化を制御するエピジェネティックな機構

    研究課題/領域番号:25830133  2013年04月 - 2015年03月

    日本学術振興会  科学研究費助成事業  若手研究(B)

    板東 哲哉

      詳細を見る

    担当区分:研究代表者  資金種別:競争的資金

    配分額:4160000円 ( 直接経費:3200000円 、 間接経費:960000円 )

  • 器官再生における遺伝子発現のエピジェネティックな制御

    研究課題/領域番号:23710217  2011年04月 - 2013年03月

    日本学術振興会  科学研究費助成事業  若手研究(B)

    板東 哲哉

      詳細を見る

    担当区分:研究代表者  資金種別:競争的資金

    配分額:4550000円 ( 直接経費:3500000円 、 間接経費:1050000円 )

  • Dachsous/Fatシグナリング系を介した位置情報決定メカニズムの解明

    研究課題/領域番号:22124003  2010年04月 - 2015年03月

    日本学術振興会  科学研究費助成事業  新学術領域研究(研究領域提案型)

    野地 澄晴,大内 淑代,三戸 太郎,板東 哲哉

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    担当区分:研究分担者  資金種別:競争的資金

    配分額:98670000円 ( 直接経費:75900000円 、 間接経費:22770000円 )

  • 脳の”眼”を構成するオプシン5視細胞の機能解明とその発生分化メカニズム

    研究課題/領域番号:24500382  2012年04月 - 2015年03月

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    大内 淑代,板東 哲哉,藤田 洋史,泰江 章博,井上 順治,加藤 睦子

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    担当区分:研究分担者  資金種別:競争的資金

    配分額:5200000円 ( 直接経費:4000000円 、 間接経費:1200000円 )

  • Toll様受容体シグナルが促進する脱分化誘導因子のエピジェネティックな発現制御

    研究課題/領域番号:21K06121  2021年04月 - 2024年03月

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    板東 哲哉

      詳細を見る

    配分額:4160000円 ( 直接経費:3200000円 、 間接経費:960000円 )

    フタホシコオロギの後脚を脛節で切断すると、創傷部位がかさぶたに覆われ、創傷部周辺にToll受容体を発現するプラズマ細胞(昆虫のマクロファージ)が遊走する。プラズマ細胞はサイトカインを産生して周辺の細胞分裂を増加させ、再生芽の形成を促進する。傷口へのプラズマ細胞の遊走は、微生物等の感染ではなく、障害された細胞由来のDAMPsがきっかけであった(Bando et al., Development, 2021 (in press)、板東ら, 昆虫と自然, 2021)。
    再生時におけるプラズマ細胞の機能をより詳細に解析するため、プラズマ細胞から産生される活性酸素種(ROS)に着目した。細胞外にROSを産生するNADPH oxidasesは、コオロギではNox5とDuoxの2遺伝子にコードされている。Nox5(RNAi)個体は正常に再生したが、Duox(RNAi)個体では再生芽の肥大や幼虫成長の不全が観察された。Duox maturation factor (DuoxA)に対するRNAi個体でも再生能の低下が観察されたことから、ROSの産生は再生芽細胞の増殖制御に関わると考えられた。しかしながらNADPH oxidases阻害剤処理したコオロギでは再生芽の肥大は観察されず、再生能が低下する表現型が観察された。そこでROSに応答する転写因子複合体Keap1/Nrf2の再生過程における機能を調べた。Keap1(RNAi)個体では再生能の低下が見られたが、Nrf2(RNAi)個体は脱皮不全を起こして再生過程への影響は不明であった。Nrf2の標的遺伝子の1つcatalaseに対するRNAiを行った場合は再生能が低下したため、ROSの消去が低下して高ROS状態になっても再生能が低下すると考えられた。

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  • マクロファージにより活性化される脱分化誘導因子のエピジェネティックな発現制御

    研究課題/領域番号:18K06184  2018年04月 - 2022年03月

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    板東 哲哉

      詳細を見る

    配分額:4290000円 ( 直接経費:3300000円 、 間接経費:990000円 )

    フタホシコオロギは切断された脚を元どおりに再生できる。失われた器官の再生には再生芽の形成が必要であり、再生芽細胞は分化した細胞が脱分化した細胞であるが、再生芽の形成については不明な点が多い。コオロギの脚再生過程では、脚の切断により傷害された細胞に由来するデブリがToll受容体を発現するプラズマ細胞(昆虫のマクロファージ)に受容されていた。プラズマ細胞は創傷部位へと遊走してサイトカインを産生することでJak/STATシグナルを活性化させ、細胞増殖を亢進させて再生芽の形成を促進することを明らかにした。

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  • 器官再生において再生芽細胞への脱分化を誘導するエピジェネティックな機構

    研究課題/領域番号:15K06897  2015年04月 - 2018年03月

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    板東 哲哉, 三戸 太郎

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    配分額:5070000円 ( 直接経費:3900000円 、 間接経費:1170000円 )

    フタホシコオロギの脚を切断すると再生芽が形成され、失われた組織は再生芽細胞から再構築される。再生芽細胞は、エピジェネティックな遺伝子発現の変化により分化細胞が脱分化したものと考えられており、増殖能と多分化能を有する。ヒストンH3K27メチル化を制御するE(z)とUtxは再生芽形成に関与しなかった。一方、ヒストンH3K9アセチル化は傷口周辺に遊走してきたマクロファージによって促進され、器官再生と関連することが示唆された。マクロファージによる器官再生の促進には自然免疫経路やROSの活性化が必要であった。

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  • 器官再生において再生芽細胞の脱分化と再分化を制御するエピジェネティックな機構

    研究課題/領域番号:25830133  2013年04月 - 2015年03月

    日本学術振興会  科学研究費助成事業  若手研究(B)

    板東 哲哉

      詳細を見る

    配分額:4160000円 ( 直接経費:3200000円 、 間接経費:960000円 )

    コオロギの脚を切断すると、分化した細胞が脱分化し再生芽が形成される。再生芽ではいくつかのエピジェネティック因子の発現が上昇していた。ヒストンH3の27番目のリジン残基のメチル化を制御するE(z)とUtxに着目して解析を行ったところ、これらエピエジェネティック因子はヒストンのメチル化を制御することでパターン形成遺伝子の発現を調節し、脚再生における形態形成に寄与した。DNAメチル化を制御する因子は脚再生に貢献せず、コオロギではヒストン修飾を介したエピジェネティックな遺伝子発現調節が重要であることが分かった。

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  • 脳の”眼”を構成するオプシン5視細胞の機能解明とその発生分化メカニズム

    研究課題/領域番号:24500382  2012年04月 - 2015年03月

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    大内 淑代, 板東 哲哉, 藤田 洋史, 泰江 章博, 井上 順治, 加藤 睦子

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    配分額:5200000円 ( 直接経費:4000000円 、 間接経費:1200000円 )

    紫外光感受性視物質であるオプシン5(Opn5)発現細胞の生体内での機能と発生分化について、鳥類と哺乳類を用いて研究を行っている。本研究では、人工核酸切断酵素によるゲノム編集法を用いて、動物個体においてOpn5遺伝子を改変して、脳と眼におけるOpn5の機能と発生分化のしくみを明らかにすることを目的とした。人工核酸切断酵素として、Transcription Activator-Like Effector Nuclease (TALEN)を用いて、マウスOpn5変異マウスの作出を行った。その結果、TALEN法により遺伝子変異が効率よくおこること、Opn5変異マウスは正常に産出されることがわかった。

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  • 器官再生における遺伝子発現のエピジェネティックな制御

    研究課題/領域番号:23710217  2011年 - 2012年

    日本学術振興会  科学研究費助成事業  若手研究(B)

    板東 哲哉

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    配分額:4550000円 ( 直接経費:3500000円 、 間接経費:1050000円 )

    コオロギ幼虫の脚を切断すると、切断された領域が再生芽細胞から再生される。分化細胞と異なり、増殖能が高く多分化能を持つ再生芽細胞ではヒストン H3 の 27 番目のリジン残基のメチル化を制御するエピジェネティック因子の発現が上昇していた。RNAi によりヒストンのメチル化を阻害または亢進させると再生脚の形態が異常になり、パターン形成遺伝子の発現パターンが異常になった。ヒストンのメチル化を介したパターン形成遺伝子のエピジェネティックな発現制御が再生脚のパターン形成に重要である。

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  • Dachsous/Fatシグナリング系を介した位置情報決定メカニズムの解明

    研究課題/領域番号:22124003  2010年04月 - 2015年03月

    日本学術振興会  科学研究費助成事業  新学術領域研究(研究領域提案型)

    野地 澄晴, 大内 淑代, 三戸 太郎, 板東 哲哉

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    配分額:98670000円 ( 直接経費:75900000円 、 間接経費:22770000円 )

    昆虫の脚再生の分子メカニズムを、再生依存的RNA干渉を利用して研究した。結果から、脚再生においては、次のプロセスで、再生されると予想した。1. 脚切断により、マクロファージ様細胞が活性化。2. マ クロファージ様細胞が産生するサイトカインが未分化細胞による再生芽形成を誘導。3. 再生芽において、Wnt/BMPシグナルがEGFを誘導。エピジェネティクスを制御する遺伝子が活性化。4. EGFはdachshund/Distal-lessの発現およびDachsous/Fatシグナル系の発現制御することにより脚のパターンおよびサイズが決定。このサイズ決定機序を改変Steepnessモデルにより説明した。

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