研究者詳細 - 青山　絵理子

論文 - 青山　絵理子

分割表示 74 件中 1 - 20 件目／全件表示 >>

Positive Regulation of S-Adenosylmethionine on Chondrocytic Differentiation via Stimulation of Polyamine Production and the Gene Expression of Chondrogenic Differentiation Factors

Loc Dinh Hoang, Eriko Aoyama, Miki Hiasa, Hiroshi Omote, Satoshi Kubota, Takuo Kuboki, Masaharu Takigawa

International Journal of Molecular Sciences 24 ( 24 ) 17294 - 17294 2023年12月

掲載種別：研究論文（学術雑誌）出版者・発行元：MDPI AG

S-adenosylmethionine (SAM) is considered to be a useful therapeutic agent for degenerative cartilage diseases, although its mechanism is not clear. We previously found that polyamines stimulate the expression of differentiated phenotype of chondrocytes. We also found that the cellular communication network factor 2 (CCN2) played a huge role in the proliferation and differentiation of chondrocytes. Therefore, we hypothesized that polyamines and CCN2 could be involved in the chondroprotective action of SAM. In this study, we initially found that exogenous SAM enhanced proteoglycan production but not cell proliferation in human chondrocyte-like cell line-2/8 (HCS-2/8) cells. Moreover, SAM enhanced gene expression of cartilage-specific matrix (aggrecan and type II collagen), Sry-Box transcription factor 9 (SOX9), CCN2, and chondroitin sulfate biosynthetic enzymes. The blockade of the methionine adenosyltransferase 2A (MAT2A) enzyme catalyzing intracellular SAM biosynthesis restrained the effect of SAM on chondrocytes. The polyamine level in chondrocytes was higher in SAM-treated culture than control culture. Additionally, Alcian blue staining and RT-qPCR indicated that the effects of SAM on the production and gene expression of aggrecan were reduced by the inhibition of polyamine synthesis. These results suggest that the stimulation of polyamine synthesis and gene expression of chondrogenic differentiation factors, such as CCN2, account for the mechanism underlying the action of SAM on chondrocytes.

DOI： 10.3390/ijms242417294

researchmap

Evaluation of the Molecular Interaction Between CCN 2 Protein and Its Binding Partners: A Solid-Phase Binding 3 Assay and Surface Plasmon Resonance 招待査読

Eriko Aoyama, Masaharu Takigawa

Methods in Molecular Biology 2582 77 - 86 2023年

Imaging of Molecular Interaction Between CCN Protein 2 and Its Binding Partners: An In Situ Proximity Ligation 3 Assay of Interaction Between CCN2 and Rab14 4 in Chondrocytes 査読国際誌

Mitsuhiro Hoshijima, Eriko Aoyama, Hiroshi Kamioka, Masaharu Takigawa

Methods in Molecular Biology 2582 31 - 37 2023年

記述言語：英語掲載種別：研究論文（学術雑誌）

An in situ proximity ligation assay (PLA) enables visualization of protein interactions in fixed cells. It is a powerful method for investigating protein-protein binding of endogenously expressed proteins. To confirm binding between CCN2 and Rab14 GTPase (Rab14) in chondrocytes, we performed a PLA using chondrocytic HCS-2/8 cells. The protocol in this chapter introduces an optimized technique for visualizing intracellular interactions of CCN2 and Rab14 in fixed cells using a PLA.

DOI： 10.1007/978-1-0716-2744-0_4

PubMed

researchmap

The Evaluation of Meniscus Regenerative Effects 2 of LIPUS-Induced CCN Proteins: Induction by LIPUS of CCN2 3 and Meniscus-Related Genes in Cultured Meniscus Cells 4 and Meniscus Tissues

Yusuke Kamatsuki, Eriko Aoyama, Takayuki Furumatsu, Toshifumi Ozaki, Masaharu Takigawa

Methods in Molecular Biology 2582 223 - 235 2023年

高転移性癌細胞由来の細胞外小胞に搭載されたMMP3によるCtgf/Ccn2発現調節機能と癌転移促進(Extracellular vesicles enriched with moonlighting metalloproteinase are highly transmissive, Pro-tumorigenic, and trans-activates cellular communication network factor(CCN2/CTGF): CRISPR against cancer)

奥舎有加, 江口傑徳, Tran Manh T., 十川千春, 吉田賀弥, 板垣まみ, Taha Eman A., 小野喜章, 青山絵理子, 岡村裕彦, 小崎健一, Calderwood Stuart K., 滝川正春, 岡元邦彰

Journal of Oral Biosciences Supplement 2022 35 - 35 2022年9月

S-アデノシルメチオニンはポリアミン産生だけでなく増殖因子の遺伝子発現を促進することによって軟骨分化を促進する(S-adenosylmethionine induces chondrocytic differentiation not only as source of polyamine production but also by stimulating growth factor genes expression)

ホアンディン・ロック, 青山絵理子, 久保田聡, 窪木拓男, 滝川正春

Journal of Oral Biosciences Supplement 2022 134 - 134 2022年9月

Fibroblast Growth Factors and Cellular Communication Network Factors: Intimate Interplay by the Founding Members in Cartilage. 国際誌

Satoshi Kubota, Eriko Aoyama, Masaharu Takigawa, Takashi Nishida

International journal of molecular sciences 23 ( 15 ) 2022年8月

記述言語：英語掲載種別：研究論文（学術雑誌）

Fibroblast growth factors (FGFs) constitute a large family of signaling molecules that act in an autocrine/paracrine, endocrine, or intracrine manner, whereas the cellular communication network factors (CCN) family is composed of six members that manipulate extracellular signaling networks. FGFs and CCNs are structurally and functionally distinct, except for the common characteristics as matricellular proteins. Both play significant roles in the development of a variety of tissues and organs, including the skeletal system. In vertebrates, most of the skeletal parts are formed and grow through a process designated endochondral ossification, in which chondrocytes play the central role. The growth plate cartilage is the place where endochondral ossification occurs, and articular cartilage is left to support the locomotive function of joints. Several FGFs, including FGF-2, one of the founding members of this family, and all of the CCNs represented by CCN2, which is required for proper skeletal development, can be found therein. Research over a decade has revealed direct binding of CCN2 to FGFs and FGF receptors (FGFRs), which occasionally affect the biological outcome via FGF signaling. Moreover, a recent study uncovered an integrated regulation of FGF and CCN genes by FGF signaling. In this review, after a brief introduction of these two families, molecular and genetic interactions between CCN and FGF family members in cartilage, and their biological effects, are summarized. The molecular interplay represents the mutual involvement of the other in their molecular functions, leading to collaboration between CCN2 and FGFs during skeletal development.

DOI： 10.3390/ijms23158592

PubMed

researchmap

S-アデノシルメチオニンによる軟骨細胞分化促進作用とそのメカニズムの解析

ほあんろっく, ほあんろっく, 青山絵理子, 久保田聡, 窪木拓男, 滝川正春

日本骨代謝学会学術集会プログラム抄録集(CD-ROM) 39回 139 - 139 2021年10月

S-アデノシルメチオニンによる軟骨細胞分化促進作用とそのメカニズムの解析

ほあん・ろっく, 青山絵理子, 久保田聡, 窪木拓男, 滝川正春

日本骨代謝学会学術集会プログラム抄録集 39回 139 - 139 2021年10月

Gel-Free 3D Tumoroids with Stem Cell Properties Modeling Drug Resistance to Cisplatin and Imatinib in Metastatic Colorectal Cancer. 国際誌

Chiharu Sogawa, Takanori Eguchi, Yuri Namba, Yuka Okusha, Eriko Aoyama, Kazumi Ohyama, Kuniaki Okamoto

Cells 10 ( 2 ) 2021年2月

記述言語：英語掲載種別：研究論文（学術雑誌）

Researchers have developed several three-dimensional (3D) culture systems, including spheroids, organoids, and tumoroids with increased properties of cancer stem cells (CSCs), also called cancer-initiating cells (CICs). Drug resistance is a crucial issue involving recurrence in cancer patients. Many studies on anti-cancer drugs have been reported using 2D culture systems, whereas 3D cultured tumoroids have many advantages for assessing drug sensitivity and resistance. Here, we aimed to investigate whether Cisplatin (a DNA crosslinker), Imatinib (a multiple tyrosine kinase inhibitor), and 5-Fluorouracil (5-FU: an antimetabolite) alter the tumoroid growth of metastatic colorectal cancer (mCRC). Gene expression signatures of highly metastatic aggregative CRC (LuM1 cells) vs. low-metastatic, non-aggregative CRC (Colon26 and NM11 cells) were analyzed using microarray. To establish a 3D culture-based multiplexing reporter assay system, LuM1 was stably transfected with the Mmp9 promoter-driven ZsGreen fluorescence reporter gene, which was designated as LuM1/m9 cells and cultured in NanoCulture Plate®, a gel-free 3D culture device. LuM1 cells highly expressed mRNA encoding ABCG2 (a drug resistance pump, i.e., CSC/CIC marker), other CSC/CIC markers (DLL1, EpCAM, podoplanin, STAT3/5), pluripotent stem cell markers (Sox4/7, N-myc, GATA3, Nanog), and metastatic markers (MMPs, Integrins, EGFR), compared to the other two cell types. Hoechst efflux stem cell-like side population was increased in LuM1 (7.8%) compared with Colon26 (2.9%), both of which were markedly reduced by verapamil treatment, an ABCG2 inhibitor. Smaller cell aggregates of LuM1 were more sensitive to Cisplatin (at 10 μM), whereas larger tumoroids with increased ABCG2 expression were insensitive. Notably, Cisplatin (2 μM) and Imatinib (10 μM) at low concentrations significantly promoted tumoroid formation (cell aggregation) and increased Mmp9 promoter activity in mCRC LuM1/m9, while not cytotoxic to them. On the other hand, 5-FU significantly inhibited tumoroid growth, although not completely. Thus, drug resistance in cancer with increased stem cell properties was modeled using the gel-free 3D cultured tumoroid system. The tumoroid culture is useful and easily accessible for the assessment of drug sensitivity and resistance.

DOI： 10.3390/cells10020344

PubMed

researchmap

Liquid-based 3D Cultured Tumoroids Modeling Resistance to Cisplatin and Imatinib in Metastatic Colorectal Cancer

Chiharu Sogawa, Takanori Eguchi, Yuri Namba, Eriko Aoyama, Kazumi Ohyama, Kuniaki Okamoto

2020年12月

出版者・発行元：MDPI AG

Researchers have developed and used several three-dimensional (3D) culture systems, including spheroids, organoids, and tumoroids. Drug resistance is a crucial issue involving recurrence in cancer patients. Many studies on anticancer drugs have been done in 2D culture systems, where-as 3D cultured tumoroids have many advantages for assessing drug sensitivity and resistance. Here, we aim to investigate whether Cisplatin (a DNA crosslinker), Imatinib (a multiple tyro-sine kinase inhibitor), and 5-Fluorouracil (5-FU: an antimetabolite) alter tumoroid growth of metastatic colorectal cancer (mCRC). To establish a liquid-based 3D multiplexing reporter assay system, LuM1 (a murine mCRC cell line) was stably transfected with the Mmp9 promoter-driven ZsGreen reporter gene, which was designated as LuM1/m9 cells and cultured in NanoCulture Plate (NCP), a 3D culture device. The larger tumoroids were not sensitive to Cisplatin and ex-pressed ABCG2 (a marker of cancer stem cells, a.k.a. a drug efflux transporter), whereas smaller cell-aggregates were more sensitive to Cisplatin. Both Imatinib and Cisplatin significantly in-creased tumoroid growth (larger than 300 &mu;m2) and Mmp9 promoter activity and were not cytotoxic to the mCRC tumoroids. On the other hand, 5-FU was cytotoxic to the tumoroids and significantly inhibited tumoroid growth, although not completely. Thus, platinum resistance and imatinib resistance in mCRC were modeled using the liquid-based 3D cultured tumoroid system. The tumoroid culture is useful and easily accessible for the assessment of drug sensitivity and resistance.

DOI： 10.20944/preprints202012.0582.v1

researchmap

Roles of Interaction between CCN2 and Rab14 in Aggrecan Production by Chondrocytes. 査読国際誌

Mitsuhiro Hoshijima, Takako Hattori, Eriko Aoyama, Takashi Nishida, Satoshi Kubota, Hiroshi Kamioka, Masaharu Takigawa

International journal of molecular sciences 21 ( 8 ) 2020年4月

記述言語：英語掲載種別：研究論文（学術雑誌）

To identify proteins that cooperate with cellular communication network factor 2 (CCN2), we carried out GAL4-based yeast two-hybrid screening using a cDNA library derived from the chondrocytic cell line HCS-2/8. Rab14 GTPase (Rab14) polypeptide was selected as a CCN2-interactive protein. The interaction between CCN2 and Rab14 in HCS-2/8 cells was confirmed using the in situ proximity ligation assay. We also found that CCN2 interacted with Rab14 through its IGFBP-like domain among the four domains in CCN2 protein. To detect the colocalization between CCN2 and Rab14 in the cells in detail, CCN2, wild-type Rab14 (Rab14WT), a constitutive active form (Rab14CA), and a dominant negative form (Rab14DN) of Rab14 were overexpressed in monkey kidney-tissue derived COS7 cells. Ectopically overexpressed Rab14 showed a diffuse cytosolic distribution in COS7 cells; however, when Rab14WT was overexpressed with CCN2, the Rab14WT distribution changed to dots that were evenly distributed within the cytosol, and both Rab14 and CCN2 showed clear colocalization. When Rab14CA was overexpressed with CCN2, Rab14CA and CCN2 also showed good localization as dots, but their distribution was more widespread within cytosol. The coexpression of Rab14DN and CCN2 also showed a dotted codistribution but was more concentrated in the perinuclear area. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis revealed that the reduction in RAB14 or CCN2 mRNA by their respective siRNA significantly enhanced the expression of ER stress markers, BIP and CHOP mRNA in HCS-2/8 chondrocytic cells, suggesting that ER and Golgi stress were induced by the inhibition of membrane vesicle transfer via the suppression of CCN2 or Rab14. Moreover, to study the effect of the interaction between CCN2 and its interactive protein Rab14 on proteoglycan synthesis, we overexpressed Rab14WT or Rab14CA or Rab14DN in HCS-2/8 cells and found that the overexpression of Rab14DN decreased the extracellular proteoglycan accumulation more than the overexpression of Rab14WT/CA did in the chondrocytic cells. These results suggest that intracellular CCN2 is associated with Rab14 on proteoglycan-containing vesicles during their transport from the Golgi apparatus to endosomes in chondrocytes and that this association may play a role in proteoglycan secretion by chondrocytes.

DOI： 10.3390/ijms21082769

PubMed

researchmap

高転移性癌細胞由来の細胞外小胞に搭載されたMMP3によるCtgf/Ccn2発現調節機能と癌転移促進

奥舎有加, 奥舎有加, 江口傑徳, 江口傑徳, TRAN Manh T., 十川千春, 吉田賀弥, 板垣まみ, 板垣まみ, TAHA Eman A., TAHA Eman A., TAHA Eman A., 小野喜章, 青山絵理子, 岡村裕彦, 小崎健一, CALDERWOOD Stuart K, 滝川正春, 岡元邦彰

Journal of Oral Biosciences Supplement (Web) 12 ( 4 ) 881 - 881 2020年4月

Extracellular Oncosomes Rich in Moonlighting Metalloproteinase (MMP3) Are Transmissive, Pro-Tumorigenic, and Induces Cellular Communication Network Factor 2 (CCN2/CTGF): CRISPR against Cancer

Yuka Okusha, Takanori Eguchi, Manh Tien Tran, Chiharu Sogawa, Kaya Yoshida, Mami Itagaki, Eman Ahmed Taha, Kisho Ono, Eriko Aoyama, Hirohiko Okamura, Ken-ichi Kozaki, Stuart K. Calderwood, Masaharu Takigawa, Kuniaki Okamoto

Preprints doi: 10.20944/preprints202002.0281.v1 2020年2月

Jiadifenolide induces the expression of cellular communication network factor (CCN) genes, and CCN2 exhibits neurotrophic activity in neuronal precursor cells derived from human induced pluripotent stem cells. 査読国際誌

Shoji M(corresponding author, Ueda M, Nishioka M, Minato H, Seki M, Harada K, Kubo M, Fukuyama Y, Suzuki Y, Aoyama E, Takigawa M, Kuzuhara T

Biochemical and biophysical research communications 519 ( 2 ) 309 - 315 2019年11月

低出力パルス超音波(LIPUS)の半月板修復効果とその作用機序 CCN2/CTGFの関与

青山絵理子, 西田崇, 久保田聡, 滝川正春, 釜付祐輔, 古松毅之, 前原亜美, 尾崎敏文, 山中信康

Journal of Oral Biosciences Supplement 2019 403 - 403 2019年10月

Possible reparative effect of low-intensity pulsed ultrasound (LIPUS) on injured meniscus. 査読国際誌

Kamatsuki Y, Aoyama E, Furumatsu T, Miyazawa S, Maehara A, Yamanaka N, Nishida T, Kubota S, Ozaki T, Takigawa M

Journal of cell communication and signaling 13 ( 2 ) 193 - 207 2019年6月

Glutathione accelerates osteoclast differentiation and inflammatory bone destruction. 査読国際誌

Fujita H, Ochi M, Ono M, Aoyama E, Ogino T, Kondo Y, Ohuchi H

Free radical research 53 ( 2 ) 1 - 11 2019年3月

CCN2/CTGF binds the small leucine rich proteoglycan protein Tsukushi 査読

Ohta K, Aoyama E, Ahmad SAI, Ito N, Anam MB, Kubota S, Takigawa M

Journal of Cell Communication and Signaling 13 ( 1 ) 113 - 118 2019年3月

Depletion of Lipid Efflux Pump ABCG1 Triggers the Intracellular Accumulation of Extracellular Vesicles and Reduces Aggregation and Tumorigenesis of Metastatic Cancer Cells. 査読

Namba Y, Sogawa C, Okusha Y, Kawai H, Itagaki M, Ono K, Murakami J, Aoyama E, Ohyama K, Asaumi JI, Takigawa M, Okamoto K, Calderwood SK, Kozaki KI, Eguchi T

Frontiers in oncology 8 376 2018年

　前のページ - 次のページ　

<青山 絵理子>

論文 - 青山 絵理子

<青山　絵理子>

論文 - 青山　絵理子