Research Projects -
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難治性網膜疾患の遺伝子治療を目的としたボルナウイルスベクターの開発
Grant number：23K09027 2023.04 - 2026.03
日本学術振興会 科学研究費助成事業 基盤研究(C)
塩出 雄亮, 森實 祐基, 本田 知之
Grant amount：\4810000 （ Direct expense: \3700000 、 Indirect expense：\1110000 ）
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Clarification of the mechanism for endogenous retrovirus to get involved in the antitumor immunity response
Grant number：22K19561 2022.06 - 2025.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)
豊岡 伸一, 冨樫 庸介, 本田 知之, 冨田 秀太, 山本 寛斉, 諏澤 憲, 枝園 和彦
Grant amount：\6370000 （ Direct expense: \4900000 、 Indirect expense：\1470000 ）
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コウモリにおける内在性ウイルス様配列によるフィロウイルス耐性の解明
Grant number：22K06027 2022.04 - 2025.03
日本学術振興会 科学研究費助成事業 基盤研究(C)
小川 寛人, 本田 知之
Grant amount：\4160000 （ Direct expense: \3200000 、 Indirect expense：\960000 ）
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Comprehensive analysis of roles of endogenous virome during virus infection
Grant number：21H02738 2021.04 - 2024.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B) Grant-in-Aid for Scientific Research (B)
本田 知之
Authorship：Principal investigator
Grant amount：\17420000 （ Direct expense: \13400000 、 Indirect expense：\4020000 ）
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セシウムがインフルエンザウイルス・RSウイルス感染に及ぼす影響
Grant number：20K08180 2020.04 - 2023.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)
山下 信子, 小川 寛人, 八代 将登, 難波 ひかる, 本田知之
Authorship：Coinvestigator(s)
Grant amount：\4160000 （ Direct expense: \3200000 、 Indirect expense：\960000 ）
今年度は、安定同位体セシウム（Cesium；Cs）添加時の細胞毒性評価とインフルエンザウイルス（IAV）の増殖に及ぼす影響を検討した。細胞毒性評価はMTT（3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide）assayを用いた。MEM培地にCsClを０, 0.1, 0.3，1, 3, 10, 30mMで添加した場合の吸光度（A570- A650）で評価し、A549細胞とHEK293T細胞で検討を行った。添加48時間後ではCsCl 添加 0.1～3ｍＭの生細胞率はA549細胞とHEK293T細胞のいずれも80％を超えていた（CsCl (-)を100％とした場合）。しかし、CsCl 10mMを超えると著しい細胞障害が認められた。次に、細胞障害を起こさない低濃度のＣsＣl添加時(0, 0.1, 0.5, 1ｍM)のポリメラーゼ活性への影響を検討するために、インフルエンザウイルスのミニゲノムアッセイ（pCAGGS-PB2,PB1,PA, NP (A/WSN/1933(H1N1))とNP分節NCRを持つルシフェラーゼ遺伝子をコードするpPolI-NP(0)Fluc(0) をHEK293T細胞にトランスフェクションし、転写・翻訳されるルシフェラーゼを測定）を行った。Relative Luciferase Activityは、CsCl 0ｍMを1とした場合、0.1ｍM 1.47、0.5ｍM 1.14、1ｍM 1.14（mean）であり有意差は認められなかった。このことから低濃度CsCｌ添加は、IAVのポリメラーゼ活性には影響を及ぼさないと考えられた。
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Elucidation of biological significance of interactions between retroelements and hosts
Grant number：18K19449 2018.06 - 2021.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)
Honda Tomoyuki
Grant amount：\6240000 （ Direct expense: \4800000 、 Indirect expense：\1440000 ）
In this study, we elucidated the biological significance of retroelement-host interactions. Among the retroelements, we found that an endogenous bornavirus-like element exhibits anti-bornavirus activity, an endogenous retrovirus regulates innate immunity, and retrotransposon LINE-1 regulates cell proliferation. We also demonstrated that oncoviruses may develop cancer by disrupting the retroelement-host interaction and enhancing the retrotransposition activity of LINE-1.
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DNA損傷修復系による核内ウイルス制御の普遍原理の解明
2018 - 2021
基盤研究(B)
本田 知之
Authorship：Principal investigator Grant type：Competitive
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Elucidation of interactions between paramyxoviral infection and host innate immunity
Grant number：16H05197 2016.04 - 2019.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Irie Takashi, SAKAI kouji, AMI yasushi
Grant amount：\17550000 （ Direct expense: \13500000 、 Indirect expense：\4050000 ）
Host innate immune system is one of the primal host defense mechanism against invading pathogens, which is activated by recognizing pathogen-associated molecular patterns (PAMPs). In this study, we tried to elucidate virus-host interactions, including interactions between virus and host innate immunity, by using Sendai virus, a prototype of mononegaviruses.
We demonstrated 1. detailed functional maps of SeV C and V proteins in the context of real viral infection, 2. relationship between SeV infection and cell death events, such as apoptosis and necroptosis, 3. virus-derived molecules which strongly induce host innate immune responses and a possible mechanism of their production, 4. possible applications of our novel findings for developments of improved SeV vectors and highly effective vaccine adjuvants. -
Spatial-temporal virus dynamics based on reaction-diffusion model
Grant number：16K13777 2016.04 - 2019.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
Iwami Shingo
Grant amount：\3510000 （ Direct expense: \2700000 、 Indirect expense：\810000 ）
Collaboration with theory and data analysis is establishing mainly in research using ODE. Currently, although development of measurement technology has made it possible to acquire rich spatio-temporal data, the theory based on ODE can not be applied to data including such spatial information. In this research project, we developed spatio-temporal data analysis approach using Borna virus infection experiments and numerical simulation.
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レトロトランスポゾンを介した新しい宿主-RNAウイルス間相互作用の探索と解析
2015 - 2018
基盤研究(Ｃ)
本田 知之
Authorship：Principal investigator Grant type：Competitive
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Infection memory: study on antiviral functions of endogenous viruses
Grant number：26253027 2014.04 - 2017.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
Tomonaga Keizo, HONDA Tomoyuki
Grant amount：\40560000 （ Direct expense: \31200000 、 Indirect expense：\9360000 ）
It is suggested that our life-form has a mechanism to memorize virus infections as endogenous element in the genomes and uses them as novel genes on evolution. This study was carried out to understand the functions, especially antiviral activity, of endogenous bornavirus-like elements (EBLs) in mammalian genomes, which we found in a previous report. In this study, we demonstrated the expression regulation of EBLs in human genomes, as well as function of a human EBL to regulate neighboring gene expression. Furthermore, we showed that EBLs from mouse and Thirteen-liked ground squirrel genomes express piwi-interacting RNA and protein, respectively, and could act as antiviral factors against bornavirus infection in cultured cells. Moreover, we revealed the possibility that EBLs endogenized in the genome of the Eptesicus genus bat genome encode a functional RNA dependent RNA polymerase derived from ancient bornavirus infection.
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核内ウイルスRNAに対する宿主認識・応答機構の解明
2013 - 2015
新学術領域研究(研究領域提案型)
本田 知之
Authorship：Principal investigator Grant type：Competitive
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核内RNAウイルスと宿主DNA損傷の相互作用解析
2013 - 2015
若手研究(B)
本田 知之
Authorship：Principal investigator Grant type：Competitive
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動物由来ＲＮＡウイルスが制御する非コードＲＮＡマシナリーの探索と解析
2012 - 2014
新学術領域研究(研究領域提案型)
本田 知之
Authorship：Principal investigator Grant type：Competitive
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モービリウイルスー宿主間の新しい相互作用機構の解明
2011 - 2012
若手研究（B）
本田 知之
Authorship：Principal investigator Grant type：Competitive
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ウイルスを用いた自閉症小脳病態の分子メカニズムの解明
2010 - 2011
研究活動スタート支援
本田 知之
Authorship：Principal investigator Grant type：Competitive
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ウイルス感染を用いた脳高次機能の制御機構の解明
2007 - 2010
特別研究員奨励費
本田 知之
Authorship：Principal investigator Grant type：Competitive
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Study on pathogenesis of psychiatric disorders using virus infections
2007
Grant type：Competitive
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神経回路網形成におけるシナプス結合の新しい接着機構の解明
2001 - 2003
特別研究員奨励費
本田 知之
Authorship：Principal investigator Grant type：Competitive
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Study on new adhesion mechanism at synapse
2000 - 2006
Grant type：Competitive