2021/04/08 更新

写真a

イケガメ ミカ
池亀 美華
IKEGAME Mika
所属
医歯薬学域 准教授
職名
准教授
外部リンク

学位

  • 歯学博士 ( 新潟大学 )

研究キーワード

  • 細胞生物学

  • 微細構造

  • Bone tissue

  • Morphology

  • Cell biology

  • ultrastructure

  • 骨組織

  • 形態学

研究分野

  • ライフサイエンス / 解剖学

学歴

  • 新潟大学   Graduate School, Division of Dental Research   Oral Anatomy

    - 1988年

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  • 新潟大学    

    - 1988年

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    国名: 日本国

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  • 新潟大学   歯学部   歯学科

    - 1986年

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    国名: 日本国

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  • 新潟大学   Faculty of Dentistry  

    - 1986年

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経歴

  • - Associate Professor,Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,Okayama University

    2004年

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  • - 岡山大学医歯薬学総合研究科 准教授

    2004年

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  • - Associate Professor,Okayama Univiersity Graduate School of Medicine , Dentistry and Pharmaceutical Sciences

    2004年

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  • - 岡山大学 大学院医歯薬学総合研究科 准教授

    2004年

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  • 新潟大学

    1996年 - 2004年

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  • Research Associate,Niigata University

    1996年 - 2004年

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  • Part-time researcher for university or other academic organization, Niigata University

    1995年 - 1996年

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  • 新潟大学 大学等非常勤研究員

    1995年 - 1996年

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  • Visiting research fellow,St. Vincent''s Institute of Medical Research

    1992年 - 1995年

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  • セント・ヴィンセント医学研究所 客員研究員

    1992年 - 1995年

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  • 新潟大学

    1988年 - 1989年

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  • Research Associate,Niigata University

    1988年 - 1989年

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所属学協会

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委員歴

  • 歯科基礎医学会   評議委員  

    2008年   

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    団体区分:学協会

    歯科基礎医学会

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  • 岡山歯学会   座長  

    2005年   

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    団体区分:学協会

    岡山歯学会

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書籍等出版物

  • 平成22年度JAROS宇宙環境利用の展望

    宇宙環境利用研究開発機構(JAROS)  2011年 

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  • 平成16〜17年度科学研究費補助金(基盤C)研究成果報告書

    2006年 

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  • Study on Biomineralization in Niigata University

    Niigata University  2001年 

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  • Study on Biomineralization in Niigata University

    Niigata University  2001年 

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  • クインテッセンス Dental Implanology 8(4)

    デンタルダイヤモンド社  2001年 

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  • 新・分子骨代謝学と骨粗鬆症

    メディカルビュー社  2001年 

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  • クインテッセンス Dental Implanology 7(5)

    デンタルダイヤモンド社  2000年 

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  • クインテッセンスDental Implantology 6(5)

    デンタルダイヤモンド社  1999年 

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  • カルシトニン受容体 b.アイソフォーム

    「カルシトニン-基礎と臨床」ライフサイエンス出版 

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  • 骨に対する作用(骨吸収に対する作用) a.形態学的研究(共著)

    「カルシトニン-基礎と臨床」ライフサイエンス出版 

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  • 破骨細胞と骨芽細胞の分化・活性化における細胞間、細胞・基質間相互作用に関する形態学的見解(共著)

    「骨代謝調節因子-最近の進歩」羊土社 

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MISC

  • Effects of hyperglycemia on bone metabolism and bone matrix in goldfish scales

    Kei-ichiro Kitamura, Tadashi Andoh, Wakana Okesaku, Yuya Tazaki, Kazuhiro Ogai, Kayo Sugitani, Isao Kobayashi, Nobuo Suzuki, Wenxi Chen, Mika Ikegame, Atsuhiko Hattori

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY203   152 - 158   2017年1月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE INC  

    Increased risk of fracture associated with type 2 diabetes has been a topic of recent concern. Fracture rislc is related to a decrease in bone strength, which can be affected by bone metabolism and the quality of the bone. To investigate the cause of the increased fracture rate in patients with diabetes through analyses of bone metabolism and bone matrix protein properties, we used goldfish scales as a bone model for hyperglycemia. Using the scales of seven alloxan-treated and seven vehicle-treated control goldfish, we assessed bone metabolism by analyzing the activity of marker enzymes and mRNA expression of marker genes, and we measured the change in molecular weight of scale matrix proteins with SDS-PAGE. After only a 2-week exposure to hyperglycemia, the molecular weight of alpha- and beta-fractions of bone matrix collagen proteins changed incrementally in the regenerating scales of hyperglycemic goldfish compared with those of euglycemic goldfish. In addition, the relative ratio of the gamma-fraction significantly increased, and a delta-fraction appeared after adding glyceraldehyde a candidate for the formation of advanced glycation end products in diabetes to isolated type 1 collagen in vitro. The enzymatic activity and mRNA expression of osteoblast and osteoclast markers were not significantly different between hyperglycemic and euglycemic goldfish scales. These results indicate that hyperglycemia is likely to affect bone quality through glycation of matrix collagen from an early stage of hyperglycemia. Therefore, non-enzymatic glycation of collagen fibers in bone matrix may lead to the deterioration of bone quality from the onset of diabetes. (C) 2016 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.cbpa.2016.09.010

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  • Effects of hyperglycemia on bone metabolism and bone matrix in goldfish scales

    Kei-ichiro Kitamura, Tadashi Andoh, Wakana Okesaku, Yuya Tazaki, Kazuhiro Ogai, Kayo Sugitani, Isao Kobayashi, Nobuo Suzuki, Wenxi Chen, Mika Ikegame, Atsuhiko Hattori

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY203   152 - 158   2017年1月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE INC  

    Increased risk of fracture associated with type 2 diabetes has been a topic of recent concern. Fracture rislc is related to a decrease in bone strength, which can be affected by bone metabolism and the quality of the bone. To investigate the cause of the increased fracture rate in patients with diabetes through analyses of bone metabolism and bone matrix protein properties, we used goldfish scales as a bone model for hyperglycemia. Using the scales of seven alloxan-treated and seven vehicle-treated control goldfish, we assessed bone metabolism by analyzing the activity of marker enzymes and mRNA expression of marker genes, and we measured the change in molecular weight of scale matrix proteins with SDS-PAGE. After only a 2-week exposure to hyperglycemia, the molecular weight of alpha- and beta-fractions of bone matrix collagen proteins changed incrementally in the regenerating scales of hyperglycemic goldfish compared with those of euglycemic goldfish. In addition, the relative ratio of the gamma-fraction significantly increased, and a delta-fraction appeared after adding glyceraldehyde a candidate for the formation of advanced glycation end products in diabetes to isolated type 1 collagen in vitro. The enzymatic activity and mRNA expression of osteoblast and osteoclast markers were not significantly different between hyperglycemic and euglycemic goldfish scales. These results indicate that hyperglycemia is likely to affect bone quality through glycation of matrix collagen from an early stage of hyperglycemia. Therefore, non-enzymatic glycation of collagen fibers in bone matrix may lead to the deterioration of bone quality from the onset of diabetes. (C) 2016 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.cbpa.2016.09.010

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  • Effects of low-intensity pulsed ultrasound on osteoclasts

    Taizo Hanmoto, Yoshiaki Tabuchi, Mika Ikegame, Takashi Kondo, Kei Ichiro Kitamura, Masato Endo, Isao Kobayashi, Hiroyuki Mishima, Toshio Sekiguchi, Makoto Urata, Azusa Seki, Sachiko Yano, Atsuhiko Hattori, Nobuo Suzuki

    Biomedical Research, Biomedical Research (Japan)38 ( 1 ) 71 - 77   2017年

  • Reduction of protein phosphatase 2A Cα promotes in vivo bone formation and adipocyte differentiation

    Kaya Yoshida, Jumpei Teramachi, Kenta Uchibe, Mika Ikegame, Lihong Qiu, Di Yang, Hirohiko Okamura

    Molecular and Cellular Endocrinology   216 - 222   2017年

  • キンギョ鱗における多核破骨細胞形成に関する形態学的研究:筋肉内自家移植鱗を用いた解析

    池亀美華, 村埜淑恵, 國定勇希, 服部淳彦, 鈴木信雄, 山本敏男

    岡山歯学会雑誌35 ( 2 ) 37 - 42   2017年

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  • Determination of cell fate in skeletal muscle following BMP gene transfer by in vivo electroporation

    Mariko Kawai, Yu-Ki Ohmori, Mai Nishino, Masayo Yoshida, Kaori Tabata, Do-Saku Hirota, Ayako Ryu-Mon, Hiromitsu Yamamoto, Junya Sonobe, Yo-Hei Kataoka, Noriko Shiotsu, Mika Ikegame, Hiroki Maruyama, Toshio Yamamoto, Kazuhisa Bessho, Kiyoshi Ohura

    EUROPEAN JOURNAL OF HISTOCHEMISTRY61 ( 2 ) 65 - 70   2017年

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    記述言語:英語   出版者・発行元:PAGEPRESS PUBL  

    We previously developed a novel method for gene transfer, which combined a non-viral gene expression vector with transcutaneous in vivo electroporation. We applied this method to transfer the bone morphogenetic protein (BMP) gene and induce ectopic bone formation in rat skeletal muscles. At present, it remains unclear which types of cells can differentiate into osteogenic cells after BMP gene transfer by in vivo electroporation. Two types of stem cells in skeletal muscle can differentiate into osteogenic cells: muscle-derived stem cells, and bone marrow-derived stem cells in the blood. In the present study, we transferred the BMP gene into rat skeletal muscles. We then stained tissues for several muscle-derived stem cell markers (e.g., Pax7, M-cadherin), muscle regeneration-related markers (e.g., Myod1, myogenin), and an inflammatory cell marker (CD68) to follow cell differentiation over time. Our results indicate that, in the absence of BMP, the cell population undergoes muscle regeneration, whereas in its presence, it can differentiate into osteogenic cells. Commitment towards either muscle regeneration or induction of ectopic bone formation appears to occur five to seven days after BMP gene transfer.

    DOI: 10.4081/ejh.2017.2772

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  • キンギョ鱗の骨組織研究への応用

    池亀美華, 服部淳彦, 鈴木信雄

    The Bone31 ( 2 ) 3 - 8   2017年

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  • Benz[a]anthracene Decreases Plasma Calcium Levels Resulting from Influence of Scale

    Nobuo Suzuki, Jun Nakano, Kimi Kawabe, Akira Toriba, Kazuichi Hayakawa, Ning Tang

    International Journal of Zoological Investigations3 ( 1 ) 72 - 81   2017年

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  • Morphological and Functional Analyses of the Tight Junction in the Palatal Epithelium of Mouse

    Noriko Shiotsu, Tadafumi Kawamoto, Mariko Kawai, Mika Ikegame, Yasuhiro Torii, Hiroyuki Sasaki, Toshio Yamamoto

    ACTA HISTOCHEMICA ET CYTOCHEMICA50 ( 4 ) 119 - 125   2017年

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    記述言語:英語   出版者・発行元:JAPAN SOC HISTOCHEMISTRY & CYTOCHEMISTRY  

    Tight junction (TJ) is one of the cell-cell junctions and known to have the barrier and fence functions between adjacent cells in both simple and stratified epithelia. We examined the distribution pattern, constitutive proteins, and permeability of TJ in the stratified squamous epithelium of the palatal mucosa of mice. Ultrastructural observations based on the ultrathin section and freeze-fracture methods revealed that poorly developed TJs are located at the upper layer of the stratum granulosum. The positive immunofluorescence of occludin (OCD), claudin (CLD)-1 and -4 were localized among the upper layer of the stratum granulosum showing a dot-like distribution pattern. And CLD-1 and -4 were localized among the stratum spinosum and the lower part of stratum granulosum additionally showed a positive reaction along the cell profiles. Western blotting of TJ constitutive proteins showed OCD, CLD-1, -2, -4, and -5 bands. The permeability test using biotin as a tracer revealed both the areas where biotin passed through beyond OCD positive points and the areas where biotin stopped at OCD positive points. These results show that poor TJs localize at the upper layer of the stratum granulosum of the palatal epithelium, and the TJs are leaky and include at least CLD-1 and -4.

    DOI: 10.1267/ahc.17006

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  • Low-intensity pulsed ultrasound induces apoptosis in osteoclasts: Fish scales are a suitable model for the analysis of bone metabolism by ultrasound

    Nobuo Suzuki, Taizo Hanmoto, Sachiko Yano, Yukihiro Furusawa, Mika Ikegame, Yoshiaki Tabuchi, Takashi Kondo, Kei-ichiro Kitamura, Masato Endo, Toshio Yamamoto, Toshio Sekiguchi, Makoto Urata, Yuko Mikuni-Takagaki, Atsuhiko Hattori

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY195   26 - 31   2016年5月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE INC  

    Using fish scales in which osteoclasts and osteoblasts coexist on the calcified bone matrix, we examined the effects of low-intensity pulsed ultrasound (LIPUS) on both osteoclasts and osteoblasts. At 3 h of incubation after LIPUS treatment, osteoclastic markers such as tartrate-resistant acid phosphatase (TRAP) and cathepsin K mRNA expressions decreased significantly while mRNA expressions of osteoblastic markers, osteocalcin, distal less homeobox 5, runt-related transcription factor 2a, and runt-related transcription factor 2b, increased significantly. At 6 and 18 h of incubation, however, both osteoclastic and osteoblastic marker mRNA expression did not change at least present conditions. Using GeneChip analysis of zebrafish scales treated with LIPUS, we found that cell death-related genes were upregulated with LIPUS treatment. Real-time PCR analysis indicated that the expression of apoptosis-related genes also increased significantly. To confirm the involvement of apoptosis in osteoclasts with LIPUS, osteoclasts were induced by autotransplanting scales in goldfish. Thereafter, the DNA fragmentation associated with apoptosis was detected in osteoclasts using the TUNEL (TdT-mediated dUTP nick end labeling) method. The multi-nuclei of TRAP-stained osteoclasts in the scales were labeled with TUNEL. TUNEL staining showed that the number of apoptotic osteoclasts in goldfish scales was significantly elevated by treatment with LIPUS at 3 h of incubation. Thus, we are the first to demonstrate that LIPUS directly functions to osteoclasts and to conclude that LIPUS directly causes apoptosis in osteoclasts shortly after exposure. (C) 2016 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.cbpa.2016.01.022

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  • Changes in Metallothionein Isoform Expression in the Bones of Ovariectomized Rats

    Chiharu Sogawa, Mika Ikegame, Ikuko Miyazaki, Toshiaki Ara, Yasuhiro Imamura, Yuka Okusha, Kazumi Ohyama, Masato Asanuma, Norio Sogawa, Toshio Yamamoto, Ken-ichi Kozaki

    JOURNAL OF HARD TISSUE BIOLOGY25 ( 1 ) 21 - 26   2016年1月

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    記述言語:英語   出版者・発行元:JOURNAL HARD TISSUE BIOLOGY  

    Metal-binding proteins, metallothioneins (MTs), may play important roles in bone metabolism. However, the contribution of MTs to bone metabolism remains obscure. In the present study, we investigated the expression of MT isoforms in bone cells and mRNA for MT isoforms in the tibiae following ovariectomy (OVX). The results obtained showed that MT-I/II and MT-III were expressed in osteoblasts, osteoclasts, and osteocytes 4 weeks after OVX. Peaks in the mRNA expression ratios (OVX/Sham) of MT-I/II and MT-III changed following OVX. The expression ratio of MT-I/II increased after 1 week, whereas that of MT-III increased 4 weeks after OVX. These results suggest that the contribution of MTs to bone metabolism may depend on the isoforms in the cell types and the stage after OVX.

    DOI: 10.2485/jhtb.25.21

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  • Effects of Low-Intensity Pulsed Ultrasound (LIPUS) on Osteoclasts and Osteoblasts: Analysis Using an Assay System With Fish Scale as a Model of Bone.

    J Orthop Trauma30 ( 8 ) S4-S4   2016年

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  • Fish Scale Study for Space Biology: Development of the therapeutic drug of the bone disease with a space experiment

      30   1 - 4   2016年

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  • 魚類のウロコを用いた宇宙生物学的研究:宇宙実験に基づいた骨疾患の治療薬の開発

    Space Utiliz Res30   1 - 4   2016年

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  • Tensile stress stimulates the expression of osteogenic cytokines/growth factors and matricellular proteins in the mouse cranial suture at the site of osteoblast differentiation

    Mika Ikegame, Yoshiaki Tabuchi, Yukihiro Furusawa, Mariko Kawai, Atsuhiko Hattori, Takashi Kondo, Toshio Yamamoto

    BIOMEDICAL RESEARCH-TOKYO37 ( 2 ) 117 - 126   2016年

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    記述言語:英語   出版者・発行元:BIOMEDICAL RESEARCH PRESS LTD  

    Mechanical stress promotes osteoblast proliferation and differentiation from mesenchymal stem cells (MSCs). Although numerous growth factors and cytokines are known to regulate this process, information regarding the differentiation of mechanically stimulated osteoblasts from MSCs in in vivo microenvironment is limited. To determine the significant factors involved in this process, we performed a global analysis of differentially expressed genes, in response to tensile stress, in the mouse cranial suture wherein osteoblasts differentiate from MSCs. We found that the gene expression levels of several components involved in bone morphogenetic protein, Wnt, and epithelial growth factor signalings were elevated with tensile stress. Moreover gene expression of some extracellular matrices (ECMs), such as cysteine rich protein 61 (Cyr61)/CCN1 and galectin-9, were upregulated. These ECMs have the ability to modulate the activities of cytokines and are known as matricellular proteins. Cyr61/CCN1 expression was prominently increased in the fibroblastic cells and preosteoblasts in the suture. Thus, for the first time we demonstrated the mechanical stimulation of Cyr61/CCN1 expression in osteogenic cells in an ex vivo system. These results suggest the importance of matricellular proteins along with the cytokine-mediated signaling for the mechanical regulation of MSC proliferation and differentiation into osteoblastic cell lineage in vivo.

    DOI: 10.2220/biomedres.37.117

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  • メラトニンが象牙質の組織構造,象牙芽細胞や成長線周期に及ぼす影響

    三島 弘幸, 尾﨑 真帆, 武内 昇平, 武市 和彦, 服部 淳彦, 鈴木 信雄, 筧 光夫, 松本 敬, 池亀 美華, 見明 康雄

    日本再生歯科医学会誌13 ( 1 ) 2 - 14   2016年

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  • 魚類のウロコを用いた宇宙生物学的研究:キンギョのウロコ及び骨疾患モデ

    鈴木信雄

    Space Utiliz Res29   87 - 90   2015年

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  • Development of Oral Epithelial Cell Line ROE2 with Differentiation Potential from Transgenic Rats Harboring Temperature-Sensitive Simian Virus 40 Large T-Antigen Gene

    Yoshiaki Tabuchi, Shigehito Wada, Mika Ikegame, Ayako Kariya, Yukihiro Furusawa, Nobuhiko Hoshi, Tatsuya Yunoki, Nobuo Suzuki, Ichiro Takasaki, Takashi Kondo, Yoshihisa Suzuki

    EXPERIMENTAL ANIMALS63 ( 1 ) 31 - 44   2014年1月

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    記述言語:英語   出版者・発行元:INT PRESS EDITING CENTRE INC  

    We have developed an immortalized oral epithelial cell line, ROE2, from fetal transgenic rats harboring temperature-sensitive simian virus 40 large T-antigen gene. The cells grew continuously at either a permissive temperature of 33 degrees C or an intermediate temperature of 37 degrees C. At the nonpermissive temperature of 39 degrees C, on the other hand, growth decreased significantly, and the Sub-G1 phase of the cell cycle increased, indicating that the cells undergo apoptosis at a nonpermissive temperature. Histological and immunocytochennical analyses revealed that ROE2 cells at 37 degrees C had a stratified epithelial-like morphology and expressed cytokeratins Krt4 and Krt13, marker proteins for oral nonkeratinized epithelial cells. Global-scale comprehensive nnicroarray analysis, coupled with bioinformatics tools, demonstrated a significant gene network that was obtained from the upregulated genes. The gene network contained 16 genes, including Cdkn1a, Fos, Krt13, and Prdm1, and was associated mainly with the biological process of skin development in the category of biological functions, organ development. These four genes were validated by quantitative real-time polymerase chain reaction, and the results were nearly consistent with the microarray data. It is therefore anticipated that this cell line will be useful as an in vitro model for studies such as physiological functions, as well as for gene expression in oral epithelial cells.

    DOI: 10.1538/expanim.63.31

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  • メラトニン投与による象牙質の組成や組織構造の変化に関する分析学的及び組織学的研究

    三島弘幸, 門田理佳, 尾碕真帆, 服部淳彦, 鈴木信雄, 筧光男, 松本敬, 池亀美華, 見明康雄

    日本再生歯科医学会誌12 ( 1 ) 11 - 22   2014年

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  • Genes responsive to low-intensity pulsed ultrasound in MC3T3-E1 preosteoblast cells

    Yoshiaki Tabuchi, Yuuki Sugahara, Mika Ikegame, Nobuo Suzuki, Kei-ichiro Kitamura, Takashi Kondo

    International Journal of Molecular Sciences14 ( 11 ) 22721 - 22740   2013年11月

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    記述言語:英語  

    Although low-intensity pulsed ultrasound (LIPUS) has been shown to enhance bone fracture healing, the underlying mechanism of LIPUS remains to be fully elucidated. Here, to better understand the molecular mechanism underlying cellular responses to LIPUS, we investigated gene expression profiles in mouse MC3T3-E1 preosteoblast cells exposed to LIPUS using high-density oligonucleotide microarrays and computational gene expression analysis tools. Although treatment of the cells with a single 20-min LIPUS (1.5 MHz, 30 mW/cm2) did not affect the cell growth or alkaline phosphatase activity, the treatment significantly increased the mRNA level of Bglap. Microarray analysis demonstrated that 38 genes were upregulated and 37 genes were downregulated by 1.5-fold or more in the cells at 24-h post-treatment. Ingenuity pathway analysis demonstrated that the gene network U (up) contained many upregulated genes that were mainly associated with bone morphology in the category of biological functions of skeletal and muscular system development and function. Moreover, the biological function of the gene network D (down), which contained downregulated genes, was associated with gene expression, the cell cycle and connective tissue development and function. These results should help to further clarify the molecular basis of the mechanisms of the LIPUS response in osteoblast cells. © 2013 by the authors
    licensee MDPI, Basel, Switzerland.

    DOI: 10.3390/ijms141122721

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  • Static and Dynamic Hypergravity Responses of Osteoblasts and Osteoclasts in Medaka Scales

    Sachiko Yano, Kei-ichiro Kitamura, Yusuke Satoh, Masaki Nakano, Atsuhiko Hattori, Toshio Sekiguchi, Mika Ikegame, Hiroshi Nakashima, Katsunori Omori, Kazuichi Hayakawa, Atsuhiko Chiba, Yuichi Sasayama, Sadakazu Ejiri, Yuko Mikuni-Takagaki, Hiroyuki Mishima, Hisayuki Funahashi, Tatsuya Sakamoto, Nobuo Suzuki

    ZOOLOGICAL SCIENCE30 ( 3 ) 217 - 223   2013年3月

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    記述言語:英語   出版者・発行元:ZOOLOGICAL SOC JAPAN  

    Fish scales are a form of calcified tissue similar to that found in human bone. In medaka scales, we detected both osteoblasts and osteoclasts and subsequently developed a new scale assay system. Using this system, we analyzed the osteoblastic and osteoclastic responses under 2-, 3-, and 4-gravity (G) loading by both centrifugation and vibration. After loading for 10 min, the scales from centrifugal and vibration loading were incubated for 6 and 24 hrs, respectively, after which the osteoblastic and osteoclastic activities were measured. Osteoblastic activity significantly increased under 2- to 4-G loading by both centrifugation and vibration. In contrast, we found that osteoclastic activity significantly decreased under 2- and 3-G loading in response to both centrifugation and vibration. Under 4-G loading, osteoclastic activity also decreased on centrifugation, but significantly increased under 4-G loading by vibration, concomitant with markedly increased osteoblastic activity. Expression of the receptor activator of the NF-kappa B ligand (RANKL), an activation factor of osteoclasts expressed in osteoblasts, increased significantly under 4-G loading by vibration but was unchanged by centrifugal loading. A protein sequence similar to osteoprotegerin (OPG), which is known as an osteoclastogenesis inhibitory factor, was found in medaka using our sequence analysis. The ratio of RANKL/OPG-like mRNAs in the vibration-loaded scales was significantly higher than that in the control scales, although there was no difference between centrifugal loaded scales and the control scales. Accordingly, medaka scales provide a useful model by which to analyze bone metabolism in response to physical strain.

    DOI: 10.2108/zsj.30.217

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  • BMP-2 gene transfer under various conditions with in vivo electroporation and bone induction

    Hiromitsu Yamamoto, Mariko Kawai, Noriko Shiotsu, Minori Watanabe, Yasuhiro Yoshida, Kazuomi Suzuki, Hiroki Maruyama, Jun-Ichi Miyazaki, Mika Ikegame, Kazuhisa Bessho, Toshio Yamamoto

    Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology24 ( 1 ) 49 - 53   2012年

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    記述言語:英語   出版者・発行元:Elsevier Ltd  

    In our previous study, wesuccessfully induced bone formation in rat skeletal muscle using a gene-transfer system, in which a non-viral BMP-2 expression vector was applied to the muscle by in vivo electroporation at 100 V. With the ultimate goal of applying this method to maxillofacial bone regeneration therapy, we sought to establish a safer system in which the gene is transferred into the target area with a lower voltage, but with the same efficiency. The LacZ or BMP-2 gene was transferred using in vivo electroporation under various conditions: 25-100 V, 50-200-ms loading time, and 8-128 pulses. The gene-transfer efficiency or bone induction was quantified by measuring β-galactosidase or alkaline phosphatase (ALP) activity and calcium content. Histological, immunohistochemical, and X-ray analyses were also used to examine the effect of the gene transfer. When the voltage for in vivo electroporation was lowered, the gene transfer efficiency was also reduced. However, by increasing the loading time or number of pulses, it was possible to improve the gene-transfer efficiency to the same level attained at 100 V, and in vivo electroporation under the lower voltage conditions successfully induced new bone. We have established a safe and efficient gene-transfer system for bone regeneration therapy using a non-viral BMP gene expression vector and in vivo electroporation. © 2011 Asian Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd.

    DOI: 10.1016/j.ajoms.2011.10.006

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  • 魚類のウロコを用いた宇宙生物学的研究:新規メラトニン誘導体のウロコ及び骨疾患ラットの骨代謝に対する作用

    鈴木信雄, 大森克徳, 井尻憲一, 北村敬一郎, 根本 鉄, 清水宣明, 笹山雄一, 西内 巧, 染井正徳, 池亀美華, 田畑 純, 中村正久, 近藤 隆, 古澤之裕, 松田恒平, 田渕圭章, 高崎一朗, 和田重人, 安東宏徳, 笠原春夫, 永瀬 睦, 久保田幸治, 土屋美和, 谷川直樹, 吉馴重徳, 大嶋一成, 鈴木 徹, 遠藤雅人, 竹内俊郎, 江尻貞一, 小萱康徳, 佐藤和彦, 渡邊竜太, 森部絢嗣, 三島弘幸, 前田斉嘉, 内田秀明, 田谷敏貴, 林明生, 中村貞夫, 杉立久仁代, 芹野 武, 嶋津 徹, 矢野幸子, 関 あずさ, 舟橋久幸, 奈良雅之, 服部淳彦

    Space Utiliz Res28   165 - 168   2012年

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  • 魚類のウロコを用いた評価系の開発と骨代謝研究への応用

    鈴木信雄, 舟橋久幸, 耿 啓達, 柿川真紀子, 山田外史, 廣田憲之, 北村敬一郎, 清水宣明, 早川和一, 三島弘幸, 岩坂正和, 上野照剛, 大森克徳, 矢野幸子, 池亀美華, 田渕圭章, 和田重人, 近藤隆, 服部淳彦

    まぐね7 ( 4 ) 174 - 178   2012年

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  • Parathyroid hormone 1 (1-34) acts on the scales and involves calcium metabolism in goldfish

    Nobuo Suzuki, Janine A. Danks, Yusuke Maruyama, Mika Ikegame, Yuichi Sasayama, Atsuhiko Hattori, Masahisa Nakamura, Makoto J. Tabata, Toshio Yamamoto, Ryo Furuya, Kiyofumi Saijoh, Hiroyuki Mishima, Ajai K. Srivastav, Yukihiro Furusawa, Takashi Kondo, Yoshiaki Tabuchi, Ichiro Takasaki, Vishwajit S. Chowdhury, Kazuichi Hayakawa, T. John Martin

    BONE48 ( 5 ) 1186 - 1193   2011年5月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE INC  

    The effect of fugu parathyroid hormone 1 (fugu PTH1) on osteoblasts and osteoclasts in teleosts was examined with an assay system using teleost scale and the following markers: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Synthetic fugu PTH1 (1-34) (100 pg/m1-10 ng/ml) significantly increased ALP activity at 6 h of incubation. High-dose (10 ng/ml) fugu PTH1 significantly increased ALP activity even after 18 h of incubation. In the case of TRAP activity, fugu PTH1 did not change at 6 h of incubation, but fugu PTH1 (100 pg/m1-10 ng/ml) significantly increased TRAP activity at 18 h. Similar results were obtained for human PTH (1-34), but there was an even greater response with fugu PTH1 than with human PTH. In vitro, we demonstrated that both the receptor activator of the NF-kappa B ligand in osteoblasts and the receptor activator NF-kappa B mRNA expression in osteoclasts increased significantly by fugu PTH1 treatment. In an in vivo experiment, fugu PTH1 induced hypercalcemia resulted from the increase of both osteoblastic and osteoclastic activities in the scale as well as the decrease of scale calcium contents after fugu PTH1 injection. In addition, an in vitro experiment with intramuscular autotransplanted scale indicated that the ratio of multinucleated osteoclasts/mononucleated osteoclasts in PTH-treated scales was significantly higher than that in the control scales. Thus, we concluded that PTH acts on osteoblasts and osteoclasts in the scales and regulates calcium metabolism in goldfish. (C) 2011 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bone.2011.02.004

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  • メラトニンの新規作用:骨に対する作用とその誘導体を用いた骨疾患治療薬の開発

    鈴木信雄, 関 あずさ, 染井正徳, 中村正久, 矢野幸子, 大森克徳, 池亀美華, 三島弘幸, 早川和一, 服部淳彦

    比較内分泌学37 ( 143 ) 194 - 203   2011年

  • 魚類のウロコを用いた宇宙生物学的研究:ウロコ及びマウスの頭蓋骨に対する重力応答

    鈴木信雄, 大森克徳, 井尻憲一, 北村敬一郎, 根本 鉄, 清水宣明, 笹山雄一, 西内 巧, 染井正徳, 池亀美華, 田畑 純, 中村正久, 近藤 隆, 古澤之裕, 松田恒平, 田渕圭章, 高崎一朗, 和田重人, 安東宏徳, 笠原春夫, 永瀬 睦, 久保田幸治, 土屋美和, 谷川直樹, 吉馴重徳, 大嶋一成, 鈴木徹, 遠藤雅人, 竹内俊郎, 江尻貞一, 小萱康徳, 前田斉嘉, 内田秀明, 田谷敏貴, 林明生, 中村貞夫, 杉立久仁代, 芹野武, 嶋津徹, 矢野幸子, 奈良雅之, 服部淳彦

    Space Utiliz Res27   209 - 212   2011年

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  • Osteoblast activity in the goldfish scale responds sensitively to mechanical stress

    Kei-ichiro Kitamura, Nobuo Suzuki, Yusuke Sato, Tetsu Nemoto, Mika Ikegame, Nobuaki Shimizu, Takashi Kondo, Yukihiro Furusawa, Shigehito Wada, Atsuhiko Hattori

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY156 ( 3 ) 357 - 363   2010年7月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE INC  

    The adaptive response of bone to mechanical loading in teleosts is not well understood. We recently developed a new assay system using teleost scales, which consists of osteoblasts, osteoclasts, and bone matrix protein. In this system, alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) were used as markers of osteoblasts and osteoclasts, respectively. Using this assay system, we examined the effects of mechanical loading on ALP and TRAP activity in goldfish scales. ALP activity in the scales was significantly elevated (p<0.01) by ultrasound stimuli (1 MHz, 50% duty factor, 0.5 Hz pulse repetition frequency, 60 mW/cm(2) [I(SATA)] and 6 min) after both 18 h and 24 h of incubation while TRAP activity remained unchanged. In addition, mRNA expression of both insulin-like growth factor-I (IGF-I) and estrogen receptors (ER) increased significantly, as did ALP activity. After the goldfish had been swimming for 3 days (speed: 2 body lengths/second, duration: 3 h/day), the scales' ALP activity increased significantly (p<0.01) but TRAP activity did not change. These in vitro and in vivo results strongly suggest that osteoblasts in the goldfish scale respond sensitively to mechanical stress and may be important in promoting bone formation. (C) 2010 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.cbpa.2010.03.002

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  • Endoglin Is Involved in BMP-2-Induced Osteogenic Differentiation of Periodontal Ligament Cells through a Pathway Independent of Smad-1/5/8 Phosphorylation

    Osamu Ishibashi, Mika Ikegame, Fumio Takizawa, Tatsuya Yoshizawa, Md Ali Moksed, Futabako Iizawa, Hisashi Mera, Akio Matsuda, Hiroyuki Kawashima

    JOURNAL OF CELLULAR PHYSIOLOGY222 ( 2 ) 465 - 473   2010年2月

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    記述言語:英語   出版者・発行元:WILEY-LISS  

    The periodontal ligament (PDL), a connective tissue located between the cementum of teeth and the alveolar bone of mandibula, plays a crucial role in the maintenance and regeneration of periodontal tissues. The PDL contains fibroblastic cells of a heterogeneous cell population, from which we have established several cell lines previously. To analyze characteristics unique for PDL at a molecular level, we performed cDNA microarray analysis of the PDL cells versus MC3T3-E1 osteoblastic cells. The analysis followed by validation by reverse transcription-polymerase chain reaction and immunochemical staining revealed that endoglin, which had been shown to associate with transforming growth factor (TGF)-beta and bone morphogenetic proteins (BMPs) as signaling modulators, was abundantly expressed in PDL cells but absent in osteoblastic cells. The knockdown of endoglin greatly suppressed the BMP-2-induced osteoblastic differentiation of PDL cells and subsequent mineralization. Interestingly, the endoglin knockdown did not alter the level of Smad-1/5/8 phosphorylation induced by BMP-2, while it suppressed the BMP-2-induced expression of IdI, a representative BMP-responsive gene. Therefore, it is conceivable that endoglin regulates the expression of BMP-2-responsive genes in PDL cells at some site downstream of Smad-1/5/8 phosphorylation. Alternatively, we found that Smad-2 as well as Smad-1/5/8 was phosphorylated by BMP-2 in the PDL cells, and that the BMP-2-induced Smad-2 phosphorylation was suppressed by the endoglin knockdown. These results, taken together, raise a possibility that PDL cells respond to BMP2 via a unique signaling pathway dependent on endoglin, which is involved in the osteoblastic differentiation and mineralization of the cells. J. Cell. Physiol. 222: 465-473, 2010. (C) 2009 Wiley-Liss, Inc.

    DOI: 10.1002/jcp.21968

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  • 魚類のウロコを用いた宇宙生物学的研究: 魚類のウロコにおけるホルモン応答

    鈴木信雄, 田畑 純, 大森克徳, 井尻憲一, 北村敬一郎, 根本 鉄, 清水宣明, 笹山雄一, 染井正徳, 池亀美華, 中村正久, 近藤 隆, 古澤之裕, 松田恒平, 田渕圭章, 高崎一朗, 和田重人, 安東宏徳, 笠原春夫, 永瀬 睦, 久保田幸治, 鈴木 徹, 遠藤雅人, 竹内俊郎, 江尻貞一, 小萱康徳, 前田斉嘉, 内田秀明, 田谷敏貴, 林明生, 中村貞夫, 杉立久仁代, 芹野 武, 奈良雅之, 服部淳彦

    Space Utiliz Res   2010年

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  • 魚類のウロコを用いた宇宙生物学的研究:宇宙実験に適したウロコの培養法の検討

    鈴木信雄, 田畑 純, 大森克徳, 井尻憲一, 北村敬一郎, 根本 鉄, 清水宣明, 染井正徳, 池亀美華, 中村正久, 近藤 隆, 松田恒平, 田渕圭章, 高崎一朗, 和田重人, 安東宏徳, 笠原春夫, 永瀬 睦, 久保田幸治, 鈴木 徹, 奈良雅之, 服部淳彦

    Space Utiliz Res25   166 - 169   2009年

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  • 岡山大学歯学部戦略的計画 -求められている今後の対応策-

    松香芳三, 池亀美華, 吉田登志子, 有馬太郎, 皆木省吾, 山本敏男, 高柴正悟, 窪木拓男, 北山滋雄, 滝川正春, 松尾龍二

    岡山歯学会雑誌28 ( 2 ) 123 - 130   2009年

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  • Estrogen deficiency and its effect on the jaw bones

    Sadakazu Ejiri, Mikako Tanaka, Naoko Watanabe, Rezwana Binte Anwar, Emi Yamashita, Kazuho Yamada, Mika Ikegame

    JOURNAL OF BONE AND MINERAL METABOLISM26 ( 5 ) 409 - 415   2008年9月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:SPRINGER JAPAN KK  

    Estrogen deficiency-induced postmenopausal osteoporosis has become a worldwide problem, inducing low bone mass and microarchitectural deterioration of the bone scaffolding in the vertebrae and long bones. With the prevalence of such osteoporosis on the increase, the influence of this estrogen deficiency on the jaw bones has drawn the attention of researchers and clinicians in the field of dentistry. The aim of this article is therefore to review the microstructural changes occurring after ovariectomy in the jaw bones of animal subjects. Induced estrogen deficiency clearly led to structural changes in the jaw bones and alveolar bone of animal subjects (rats and monkeys). Severe bone loss in the rat alveolar bone was principally caused by high bone resorptive activity. This activity accelerated greatly immediately after ovariectomy, and was then followed by more moderate resorptive activity, which continued over an extended period. Additionally, occlusal hypofunction further greatly accelerated the fragility of the alveolar bone structure in ovariectomized rats. Microstructural damage also seen in the alveolar bone of ovariectomized monkeys was found to be directly connected to their systemic osteoporosis. Recent investigations of the relationship in humans between systemic osteoporosis and jaw bone loss have also suggested that a connection may exist between these two. However, more research is required to confirm this connection in humans as well.

    DOI: 10.1007/s00774-008-0870-4

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  • Estrogen deficiency and its effect on the jaw bones

    Sadakazu Ejiri, Mikako Tanaka, Naoko Watanabe, Rezwana Binte Anwar, Emi Yamashita, Kazuho Yamada, Mika Ikegame

    JOURNAL OF BONE AND MINERAL METABOLISM26 ( 5 ) 409 - 415   2008年9月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:SPRINGER JAPAN KK  

    Estrogen deficiency-induced postmenopausal osteoporosis has become a worldwide problem, inducing low bone mass and microarchitectural deterioration of the bone scaffolding in the vertebrae and long bones. With the prevalence of such osteoporosis on the increase, the influence of this estrogen deficiency on the jaw bones has drawn the attention of researchers and clinicians in the field of dentistry. The aim of this article is therefore to review the microstructural changes occurring after ovariectomy in the jaw bones of animal subjects. Induced estrogen deficiency clearly led to structural changes in the jaw bones and alveolar bone of animal subjects (rats and monkeys). Severe bone loss in the rat alveolar bone was principally caused by high bone resorptive activity. This activity accelerated greatly immediately after ovariectomy, and was then followed by more moderate resorptive activity, which continued over an extended period. Additionally, occlusal hypofunction further greatly accelerated the fragility of the alveolar bone structure in ovariectomized rats. Microstructural damage also seen in the alveolar bone of ovariectomized monkeys was found to be directly connected to their systemic osteoporosis. Recent investigations of the relationship in humans between systemic osteoporosis and jaw bone loss have also suggested that a connection may exist between these two. However, more research is required to confirm this connection in humans as well.

    DOI: 10.1007/s00774-008-0870-4

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  • Prolactin Inhibits Osteoclastic Activity in the Goldfish Scale: A Novel Direct Action of Prolactin in Teleosts

    Hideya Takahashi, Nobuo Suzuki, Chiyo Takagi, Mika Ikegame, Toshio Yamamoto, Akiyoshi Takahashi, Shunsuke Moriyama, Atsuhiko Hattori, Tatsuya Sakamoto

    ZOOLOGICAL SCIENCE25 ( 7 ) 739 - 745   2008年7月

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    記述言語:英語   出版者・発行元:ZOOLOGICAL SOC JAPAN  

    In teleosts, prolactin is involved in calcium regulation, but its role in scale/bone metabolism is unclear. Using the in-vitro system with goldfish scales developed recently, we explored the effects of teleost prolactin, growth hormone, and somatolactin on osteoclasts and osteoblasts. Addition of prolactin at concentrations of 0.01-100 ng/ml reduced osteoclastic activity, partly via osteoclast apoptosis, after 6-18 h incubation. Conversely, growth hormone and somatolactin at a concentration of 100 ng/ml increased osteoclastic activity after 18 h incubation, indicating the specificity of the inhibitory effect of prolactin on osteoclastic activity. On the other hand, these three hormones promoted osteoblastic activity at concentrations of 10-100 ng/ml. The results from this study are the first demonstration of direct effects of prolactin on scale/bone metabolism and osteoclastic activity in a teleost.

    DOI: 10.2108/zsj.25.739

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  • 物理的刺激に対する骨芽細胞及び破骨細胞の応答:骨のモデルであるウロコ野in vitroアッセイシステムによる骨代謝の解析

    鈴木信雄, 清水宣明, 北村敬一郎, 根本 鉄, 染井正徳, 池亀美華, 和田重人, 近藤隆, 大森克徳, 中村正久, 井尻憲一, 田畑純, 服部淳彦

    日本生体電気・物理刺激研究会誌22   31 - 37   2008年

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  • Scale osteoblasts and osteoclasts sensitively respond to low-gravity loading by centrifuge.

    Suzuki N, Omori K, Nakamura M, Tabata MJ, Ikegame M, Ijiri K, Kitamura K, Nemoto T, Shimizu N, Kondo T, Matsuda K, Ando H, Kasahara H, Nagase M, Nara M, Hattori A

    Biol Sci Space22   3 - 7   2008年

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  • 伸展刺激による骨芽細胞の分化とアクチン細胞骨格の形態変化

    池亀美華, 江尻貞一, 山本敏男

    日本実験力学会講演論文集8   46 - 48   2008年

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  • 擬似微小重力及び過重力下における骨代謝制御:培養ウロコを用いた解析

    鈴木信雄, 大森克徳, 井尻憲一, 北村敬一郎, 清水宣明, 田畑 純, 池亀美華, 中村正久, 近藤 隆, 松田恒平, 安東宏徳, 笠原春夫, 永瀬 睦, 久保田幸治, 奈良雅之, 服部淳彦

    Space Utiliz Res24   230 - 233   2008年

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  • 物理的刺激に対する骨芽細胞及び破骨細胞の応答:骨のモデルであるウロコ野in vitroアッセイシステムによる骨代謝の解析

    鈴木信雄, 清水宣明, 北村敬一郎, 根本 鉄, 染井正徳, 池亀美華, 和田重人, 近藤隆, 大森克徳, 中村正久, 井尻憲一, 田畑純, 服部淳彦

    日本生体電気・物理刺激研究会誌22   31 - 37   2008年

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  • Two osteoclastic markers expressed in multinucleate osteoclasts of goldfish scales

    KyoIchi Azuma, Masaki Kobayashi, Masahisa Nakamura, Nobuo Suzuki, Sayaka Yashima, Shawichi Iwamuro, Mika Ikegame, Toshio Yamamoto, Atsuhiko Hattori

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS362 ( 3 ) 594 - 600   2007年10月

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    記述言語:英語   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Complementary DNAs encoding two major osteoclastic markers, tartrate-resistant acid phosphatase (TRAP) and cathepsin K (Cath K) were cloned from the scales of a teleost, the goldfish. This is the first report of the full coding sequence of TRAP and Cath K molecules in fish. In the goldfish scale both TRAP and Cath K mRNAs were expressed in the multinucleate osteoclasts, which showed large numbers of mitochondria and lysosomes, and a well developed ruffled border. These characteristic features of osteoclasts in the scales are similar to those in mammals. Most teleosts use the scale as an internal calcium reservoir during the reproductive season. The expression of TRAP and Cath K mRNAs in the scale significantly increased in April, which is a reproductive season, compared with that in October, a non-reproductive season. Thus, both of these molecular markers should be useful for the study of osteoclasts in the teleost scale. (c) 2007 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2007.08.010

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  • PIASx beta is a key regulator of osterix transcriptional activity and matrix mineralization in osteoblasts

    Md. Moksed Ali, Tatsuya Yoshizawa, Osamu Ishibashi, Akio Matsuda, Junko Shimomura, Hisashi Mera, Kazuhisa Nakashima, Hiroyuki Kawashima

    JOURNAL OF CELL SCIENCE120 ( 15 ) 2565 - 2573   2007年8月

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    記述言語:英語   出版者・発行元:COMPANY OF BIOLOGISTS LTD  

    We recently reported that tensile stress induces osteoblast differentiation and osteogenesis in the mouse calvarial suture in vitro. Using this experimental system, we identified PIASx beta, a splice isoform of Pias2, as one of the genes most highly upregulated by tensile stress. Further study using cell culture revealed that this upregulation was transient and was accompanied by upregulation of other differentiation markers, including osterix, whereas expression of Runx2 was unaffected. Runx2 and osterix are the two master proteins controlling osteoblast differentiation, with Runx2 being upstream of osterix. Targeted knockdown of PIASx beta by small interfering RNA (siRNA) markedly suppressed osteoblastic differentiation and matrix mineralization, whereas transient overexpression of PIASx beta caused the exact opposite effects. Regardless of PIASx beta expression level, Runx2 expression remained constant. Reporter assays demonstrated that osterix enhanced its own promoter activity, which was further stimulated by PIASx beta but not by its sumoylation-defective mutant. NFATc1 and NFATc3 additionally increased osterix transcriptional activity when co-transfected with PIASx beta. Because osterix has no consensus motif for sumoylation, other proteins are probably involved in the PIASx beta-mediated activation and NFAT proteins may be among such targets. This study provides the first line of evidence that PIASx beta is indispensable for osteoblast differentiation and matrix mineralization, and that this signaling molecule is located between Runx2 and osterix.

    DOI: 10.1242/jcs.005090

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  • 魚類のウロコを用いた宇宙生物学的研究

    鈴木 信雄, 大森 克徳, 井尻 憲一, 北村 敬一郎, 清水 宣明, 田畑 純, 池亀美華, 中村 正久, 近藤 隆, 松田 恒平, 安東 宏徳, 笠原 春夫, 永瀬 睦, 久保田幸治, 奈良 雅之, 服部 淳彦

    Space Utiliz. Res.23   318 - 321   2007年

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  • Relationship between porotic changes in alveolar bone and spinal osteoporosis.

    Binte Anwar R, Tanaka M, Kohno S, Ikegame M, Watanabe N, Nowazesh Ali M, Ejiri S

    J Dent Res86 ( 1 ) 52 - 57   2007年

  • Histochemical and immunocytochemical study of hard tissue formation in dental pulp during the healing process in rat molars after tooth replantation

    Hiroko Tsukamoto-Tanaka, Mika Ikegame, Ritsuo Takagi, Hidemitsu Harada, Hayato Ohshima

    CELL AND TISSUE RESEARCH325 ( 2 ) 219 - 229   2006年8月

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    記述言語:英語   出版者・発行元:SPRINGER  

    Dental pulp is assumed to possess the capacity to elaborate both bone and dentin matrix under the pathological conditions following tooth injury. This study was undertaken to clarify the mechanism inducing bone formation in the dental pulp by investigating the pulpal healing process, after tooth replantation, by micro-computed tomography (mu-CT), immunocytochemistry for heat-shock protein (HSP)-25 and cathepsin K (CK), and histochemistry for both alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP). Under deep anesthesia, the upper right first molar of 4-week-old Wistar rats was extracted and immediately repositioned in the original socket. In control teeth at this age, the periphery of the coronal dental pulp showed intense ALP-positive and HSP-25-positive reactions, whereas there were no TRAP-positive or CK-positive cells. Tooth replantation weakened or terminated ALP-positive and HSP-25-positive reactions in the pulp tissue at the initial stages. At 3-7 days after operation, the ALP-positive region recovered from the root apex to the coronal pulp followed by HSP-25-positive reactions in successful cases showing tertiary dentin formation. In other cases, TRAP-positive and CK-positive cells appeared in the pulp tissue of the replanted tooth at postoperative days 5-10 and remained associated with the bone tissue after 12-60 days. Immunoelectron microscopy clearly demonstrated that CK-positive osteoclast-lineage cells made contact with mesenchymal cells with prominent nucleoli and well-developed cell organelles. These data suggest that the appearance of TRAP-positive and CK-positive cells is involved in the induction of bone tissue formation in dental pulp.

    DOI: 10.1007/s00441-005-0138-4

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  • 岡山大学歯学部戦略的計画 教育に関する提案―岡山大学歯学部の理想的な将来のためにー.

    松香芳三, 池亀美華, 有馬太郎, 出口 徹, 志茂 剛, 松尾龍二, 高柴正悟, 渡邊達夫

    岡山歯学会雑誌   2006年

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  • 連載 最新用語解説(基礎) 骨のメカセンサー.

    池亀美華

    骨粗鬆症治療5(3), 64(248)-67(251)   2006年

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  • Ultrastructural and immunohistochemical studies of medullary bone calcification, with special reference to sulphated glycosaminoglycans

    T Yamamoto, N Nagaoka, A Hirata, H Nakamura, M Inoue, M Kawai, T Yamamoto

    JOURNAL OF ELECTRON MICROSCOPY54 ( 1 ) 29 - 34   2005年1月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Histochemical, immunohistochemical and electron energy-loss spectroscopic studies were performed to examine the relationship between sulphated glycosaminoglycans and medullary bone calcification using oestrogen-injected male Japanese quail. Sulphated glycosaminoglycans, detected by high iron diamine (HID) or HID-thiocarbohydrazide-silver protein (HID-TCH-SP) methods, were distributed throughout the matrix of medullary bone, some periphery and extending tips of the trabeculae stained weakly, and the globular structures at osteoid areas were exclusively positive for HID-TCH-SP stain. Immunohistochemistry identified keratan sulphate located in the globular structures at osteoid areas and calcified matrix, but chondroitin-4 sulphate and chondroitin-6 sulphate were not detected in the matrix. Using electron spectroscopic imaging, sulphur was determined to be localized in the globular structures. These results demonstrate that medullary bone matrix accumulates keratan sulphate in the globular structures, which are the foci for calcification, and eventually in the calcified areas. This suggests that keratan sulphate containing sulphur is maintained in the calcified matrix. These results indicate a unique process of calcification exists in medullary bone.

    DOI: 10.1093/jmicro/dfh097

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  • カルシトニン 薬理作用と生理作用 骨吸収抑制作用

    池亀美華

    「臨床分子内分泌学3」―甲状腺・副甲状腺・骨内分泌代謝系―198-201   2005年

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  • Calcitonin-induced change in serum calcium levels and its relationship to osteoclast morphology and number of calcitonin receptors

    M Ikegame, S Ejiri, H Ozawa

    BONE35 ( 1 ) 27 - 33   2004年7月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE INC  

    It has been shown that, in live subjects, the ability of calcitonin (CT) to decrease serum calcium (Ca) levels can be lost in response to its continued or repeated administration. The present study investigated the relationship between such changes of in vivo serum Ca levels and the response of osteoclasts to CT administration, including the downregulation of their CT receptors (CTRs). Rats were either given a single injection of CT or repeated injections at either 6- or 24-h intervals, after which their serum Ca levels were evaluated. Their parietal holies were dissected, and the amount of I-125-labeled elcatonin (I-125-eCT) binding to their osteoclasts measured using autoradiography. Ultrastructural changes in the osteoclasts were also examined. Twenty-four hours after a single CT administration, serum Ca levels had dropped, and there was an absence of ruffled borders on the osteoclasts. Less I-125-eCT binding to the osteoclast was found than in the control group. Forty-eight and 72 h after CT administration, serum Ca levels had almost returned to control levels, and the osteoclasts showed ruffled borders once again. The amount of I-125-eCT binding to the osteoclast also recovered to control levels. When these osteoclasts were then incubated in CT, their ruffled borders once again disappeared. In the 6-h interval multiple CT administration schedule subjects, upon inspection 72 h after their first administration (6 h following the final one), serum Ca levels were found to have almost returned to control levels with the presence of osteoclast ruffled borders. The amount of I-125-eCT binding to these osteoclasts was remarkably limited, and no disappearance of the ruffled borders occurred in response to additional CT incubation. In the 24-h interval multiple administration schedule subjects, upon inspection 72 h after their first CT administration (24 h following the final one), there was less I-125-eCT binding than in the single-dose subjects tested 24 h after their injection, and the ability of CT to lower their serum Ca levels was reduced. The ability of CT to lower serum Ca levels was therefore related to the response of osteoclasts to the CT (the disappearance of the ruffled borders), and this response was related to the amount of CTRs available for binding with CT on the osteoclast surface. Furthermore, the reduced effectiveness of CT in response to repeated CT administration was found to be related to the downregulation of the CTRs on the osteoclast surface. (C) 2004 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bone.2004.03.018

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  • Expression of osteopontin in cisplatin-induced tubular injury

    S Iguchi, S Nishi, M Ikegame, K Hoshi, T Yoshizawa, H Kawashima, M Arakawa, H Ozawa, F Gejyo

    NEPHRON EXPERIMENTAL NEPHROLOGY97 ( 3 ) 96 - 105   2004年

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    記述言語:英語   出版者・発行元:KARGER  

    Osteopontin (OPN) is considered as a key protein in cell regeneration. OPN is thought to have many functions in cell-cell binding and cell-matrix binding via OPN receptors in various organs. But there is little information on the precise role of OPN. To clarify the functional role of OPN in tubular injury, we performed in situ hybridization and immunohistochemical analysis of the expression of OPN in a renal cortical necrosis model induced by cisplatin from the acute injury to late recovery phases. In the acute injury phase, both mRNA and protein of OPN were markedly induced in damaged tubular lumens with cell debris. In the late recovery phase, on the other hand, OPN protein and mRNA were observed in dilated and flattened tubular epithelial cells showing a regenerative appearance. Most of these cells were also immunostained with CD44, a receptor of OPN. PCNA staining was also co-localized with these expressions. In light of the CD44 function regulating cell proliferation, these findings suggest that OPN may contribute to regeneration of tubular epithelial cells during the acute to late recovery phases of cortical tubular damage induced by cisplatin. Copyright (C) 2004 S. Karger AG, Basel.

    DOI: 10.1159/000078643

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  • Tensile stress induces α-adaptin C production in mouse calvariae in an organ culture: Possible involvement of endocytosis in mechanical stress-stimulated osteoblast differentiation.

    Shimomura J, Ishibashi O, Ikegame M, Yoshizawa T, Ejiri S, Noda T, Kawashima H

    J Cellular Physiol195 ( 3 ) 488 - 496   2003年6月

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  • A cell line with characteristics of the periodontal ligament fibroblasts is negatively regulated for mineralization and Runx2/Cbfa1/Osf2 activity, part of which can be overcome by bone morphogenetic protein-2

    Y Saito, T Yoshizawa, F Takizawa, M Ikegame, O Ishibashi, K Okuda, K Hara, K Ishibashi, M Obinata, H Kawashima

    JOURNAL OF CELL SCIENCE115 ( 21 ) 4191 - 4200   2002年11月

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    記述言語:英語   出版者・発行元:COMPANY OF BIOLOGISTS LTD  

    The periodontal ligament (PDL) is a connective tissue located between the cementum of teeth and the alveolar bone of the mandibula. It plays an integral role in the maintenance and regeneration of periodontal tissue. The cells responsible for maintaining this tissue are thought to be fibroblasts, which can be either multipotent or composed of heterogenous cell populations. However, as no established cell lines from the PDL are available, it is difficult to assess what type of cell promotes all of these functions. As a first step to circumvent this problem, we have cloned and characterized cell lines from the PDL from mice harboring a temperature-sensitive SV 40 large T-antigen gene. RT-PCR and in situ hybridization studies demonstrated that a cell line, designated PDL-L2, mimics the gene expression of the PDL in vivo: it expresses genes such as alkaline phosphatase, type I collagen, periostin, runt-related transcription factor-2 (Runx2) and EGF receptor, but does not express genes such as bone sialoprotein and osteocalcin. Unlike osteoblastic cells and a mixed cell population from the PDL, PDL-L2 cells do not produce mineralized nodules in the minearlization medium. When PDL-L2 cells were incubated in the presence of recombinant human bone morphogenetic protein-2 alkaline phosphatase activity increased and mineralized nodules were eventually produced, although the extent of mineralization is much less than that in osteoblastic MC3T3-E1 cells. Furthermore, PDL-L2 cells appeared to have a regulatory mechanism by which the function of Runx2 is normally suppressed.

    DOI: 10.1242/jcs.00098

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  • 破骨細胞の形態とカルシトニンの作用

    池亀美華

    Clinical Calcium11(9), 39-44   2001年

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  • Tensile stress induces bone morphogenetic protein 4 (BMP-4) in preosteoblastic and fibroblastic cells, which later differentiate into osteoblasts leading to osteogenesis in the mouse calvariae in organ culture.

    Ikegame, M, Ishibashi, O, Yoshizawa, T, Shimomura, J, Komori, T, Ozawa, H, Kawashima, H

    J. Bone Miner. Res.16 ( 1 ) 24 - 32   2001年

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  • Expression of osteopontin in gentamicin-incuced acute tubular necrosis and its recovery process.

    Xie Y, Nishi S, Iguchi S, Imai N, Sakatsume M, Saito A, Ikegame M, Iino N, Shimada H, Ueno M, Kawashima H, Arakawa M, Gejyo F

    Kidney International   2001年

  • Osteopontin localization and expression in cellular cementum at the site of root resorption during physiological tooth movement.

    Sasakura, K, Ikegame, M, Kenmotu, S, Kondo, Y, Ejiri, S, Hanada, K, Ozawa, H

    Orthodontic Wave   2001年

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  • Role of stromal cells in osteoclast differentiation in bone marrow.

    Kondo, Y, Irie, K, Ikegame, M, Ejiri, S, Hanada, K, Ozawa, H

    J Bone Miner Metab   2001年

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  • メカニカルストレスと骨芽細胞の分化および骨形成

    川島博行, 池亀美華

    生体の科学51(6), 589-595   2000年

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  • Morphology of Bone Metastasis(共著)

    European Journal of Cancer34 ( 2 ) 230 - 239   1998年2月

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  • Morphology of bone metastasis

    T. Hiraga, S. Tanaka, M. Ikegame, M. Koizumi, H. Iguchi, T. Nakajima, H. Ozawa

    European Journal of Cancer34 ( 2 ) 230 - 239   1998年2月

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    記述言語:英語   出版者・発行元:Elsevier Ltd  

    DOI: 10.1016/S0959-8049(97)10131-9

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  • Morphology of Bone Metastasis(共著)

    European Journal of Cancer34 ( 2 ) 230 - 239   1998年2月

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  • Effects of continuous calcitonin treatment on osteoclast-like cell development and calcitonin receptor expression in mouse bone marrow cultures.(共著)

    Ikegame M, Rakopoulos M, Martin TJ, Findlay DM

    Journal of Bone and Mineral Research11 ( 4 ) 456 - 465   1996年4月

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    記述言語:英語  

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  • Histochemical and autoradiographic studies on Elcatonin internalization and intracellular movement in osteoclasts.(共著)

    Ikegame M, Ejiri S, Ozawa H

    Journal of Bone and Mineral Research9 ( 1 ) 25 - 37   1994年1月

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    記述言語:英語  

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▼全件表示

講演・口頭発表等

  • 卵巣摘出ラット脛骨におけるメタロチオネインアイソフォームの発現

    メタルバイオサイエンス研究会2017 ~生体金属の陰と陽~  2017年 

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  • 機械的伸展刺激によるCCN1/CYR61発現促進へのYAP/TAZ核移行シグナルの関与

    第72回解剖学会中国・四国支部学術集会  2017年 

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  • 概日リズム同調因子メラトニンによる象牙質や象牙芽細胞の組織構造への影響

    第35回化石研究会総会・学術大会  2017年 

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  • プロテインホスファターゼPP2Aは骨肉腫細胞の増殖・転移能を制御する

    第36回分子病理研究会  2017年 

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  • 機械的伸展刺激によるCCN1/CYR61の発現促進へのYAP/TAZの関与

    第9回日本CCN ファミリー研究会  2017年 

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  • 新規メカニカルストレス応答性因子KLHDC8Aの骨組織内局在

    第59回歯科基礎医学会総会  2017年 

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  • 骨芽細胞の分化におけるプロテインホスファターゼPP2A Cαの役割とその標的因子

    第59回歯科基礎医学会総会  2017年 

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  • RARγシグナリングは成長板軟骨細胞分化・成熟を制御する

    第59回歯科基礎医学会総会  2017年 

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  • Effect of circadian rhythm synchronous factor melatonin on structure, composition, and calcification of dentin and odontoblasts.

    4th International Symposiumu on Biomineralization  2017年 

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  • ヒストン脱メチル化酵素Jmjd3による骨芽細胞のアポトーシス制御機構

    第72回解剖学会中国・四国支部学術集会  2017年 

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  • メカニカルストレスによる骨形成促進機構に関する研究

    2017 Bioscience Retreat in Tosa  2017年 

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  • キンギョ高血糖モデルにおける骨代謝および糖化ウロココラーゲンの架橋解析

    日本動物学会中部支部大会  2017年 

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  • マウス骨組織におけるメラトニン受容体の局在と日内変動に関する形態学的検討

    第71回解剖学会中国・四国支部学術集会  2016年 

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  • Suppressive effects of melatonin on osteoclastic function in chick calvaria

    Joint Meeting of The 22nd International Congress of Zoology and The 87th Meeting of The Zoological Society of Japan  2016年 

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  • 低出力超音波パルスの破骨細胞及び骨芽細胞に対する作用:魚のウロコを用いた解析

    第19回超音波骨折治療研究会  2016年 

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  • 時刻情報伝達物質メラトニンが象牙質の構造や石灰化機構に及ぼす影響

    第121回 日本解剖学会総会・全国学術集会  2016年 

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  • Morphometric analysis of osteoclast activity in the goldfish scale cultured under microgravity during spaceflight.

    第36回 日本骨形態計測学会 ミニ国際シンポジウム  2016年 

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  • RNTはARNT/HIF-1αによる血管形成とは独立して直接骨芽細胞の分化を促進する

    第34回 日本骨代謝学会学術集会  2016年 

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  • Effects of low-intensity pulsed ultrasound on osteoclasts: Analysis with goldfish scales as a model of bone

    Joint Meeting of The 22nd International Congress of Zoology and The 87th Meeting of The Zoological Society of Japan  2016年 

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  • BMP-2/7ヘテロダイマー産生プラスミドベクターを用いた歯槽骨再生モデル

    第34回 日本骨代謝学会学術集会  2016年 

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  • Correlation between the structure and composition of teeth dentin and the melatonin of circadian rhythm synchronous factor

    13th International Symposiumu on Biomineralization  2015年 

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  • 生体リズム伝達物質であるメラトニンが象牙質の組織構造に及ぼす影響

    第10回バイオミネラリゼーションワークショップ  2015年 

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  • Mechanisms of melatonin action onosteoclasts in bone tissue

    第40回日本比較内分泌学会・第37回日本比較生理生化学会 合同大会  2015年 

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  • オスウズラに形成された骨髄骨の吸収に対するプロゲステロンの影響

    第57回歯科基礎医学会学術大会・総会  2015年 

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  • 宇宙実験を基盤にした骨疾患治療薬の開発

    第15回 宇宙科学シンポジウム  2015年 

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  • 魚類のウロコを用いた宇宙生物学的研究:キンギョのウロコ及び骨疾患モデルラットの骨代謝に対するブロモメラトニンの新規作用

    第29回宇宙環境利用シンポジウム  2015年 

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  • Histological and analytical studies on the role of melatonin in the structure and composition of teeth dentin

    第120回 日本解剖学会総会・全国学術集会、第92回日本生理学会大会 合同大会  2015年 

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  • Response of osteoclasts in the regenerating scales of goldfish under microgravity during space flight

    第120回 日本解剖学会総会・全国学術集会,第92回日本生理学会大会 合同大会  2015年 

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  • Melatonin suppresses the microgravity-induced activation of osteoclasts in cultured goldfish scale

    13th Congress of the International Society of Bone Morphometry  2015年 

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  • 骨縫合部における機械的伸展刺激による骨芽細胞形成促進機構へのYAP/TAZの関与

    第57回歯科基礎医学会学術大会・総会  2015年 

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  • 口腔粘膜上皮におけるタイトジャンクション構成タンパクの局在

    第57回歯科基礎医学会学術大会・総会  2015年 

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  • in vivo electroporation を用いたBMP遺伝子導入による筋内異所性骨誘導における未分化間葉系細胞の動態について

    第57回歯科基礎医学会学術大会・総会  2015年 

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  • 機械的伸展刺激により縫合部組織において変化する細胞増殖ならびに分化関連因子の検討

    第56回歯科基礎医学会総会  2014年 

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  • 魚類及び哺乳類における新規メラトニン誘導体の骨代謝に対する作用

    日本動物学会 第85回仙台大会  2014年 

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  • 過重力及び擬似微小重力に対する破骨細胞及び骨芽細胞の応答解析

    宇宙生物科学会  2014年 

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  • MC3T3-E1 前骨芽細胞様細胞において低出力パルス超音波によって誘導される遺伝子の

    第17回 超音波骨折治療研究会  2014年 

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  • 新規メラトニン誘導体の骨代謝に対する作用:魚類及び哺乳類におけるin vivoの解析

    日本動物学会 中部支部例会  2014年 

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  • Tensile stress-responsible novel cell proliferation / differentiation factors in mouse cranial sutures

    International Symposium on Mechanobiology 2014  2014年 

     詳細を見る

  • Response of osteoclasts and receptor activator of nuclear factor kappa-B ligand expression in the regenerating scales of goldfish under microgravity.

    International Symposium on Mechanobiology 2014  2014年 

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  • 骨組織におけるメラトニンの作用機序の解明――ニワトリ胚をモデルとしてーー

    第14回日本抗加齢医学会総会  2014年 

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  • 新規メラトニン誘導体の卵巣摘出ラットに対する作用

    第38回日本比較内分泌学会大会  2013年 

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  • 擬似微小重力に対する骨モデル(ウロコ)の破骨細胞及び骨芽細胞の応答解析

    第38回日本比較内分泌学会大会  2013年 

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  • MC3T3-E1前骨芽細胞様細胞において低出力パルス超音波に応答する遺伝子の同定

    第12回日本超音波治療研究会 第2回超音波分子診断治療研究会共催  2013年 

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  • ヒトおよびニワトリ骨組織におけるメラトニン受容体の発現と局在

    日本動物学会 第84回岡山大会  2013年 

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  • 微小重力におけるウロコの破骨細胞の応答

    日本動物学会 第84回岡山大会  2013年 

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  • IDENTIFICATION OF GENES RESPONSIVE TO LOW-INTENSITY PULSED ULTRASOUND IN

    2013 ICBMU (International Conference on Biomedical Ultrasound)  2013年 

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  • 口蓋の粘膜上皮におけるタイトジャンクションについて

    第118回日本解剖学会総会・全国学術集会  2013年 

     詳細を見る

  • A comprehensive analysis of mechanical stress-regulated gene expression in mouse cranial sutures.

    2nd Joint Meeting of the International Bone and Mineral Society and the Japanese society for Bone and  2013年 

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  • ヒト骨組織におけるメラトニン受容体遺伝子の発現と局在

    第13回日本抗加齢医学会総会  2013年 

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  • 魚類ウロコ破骨細胞に対する宇宙実験と3Dクリノスタットによる微小重力の作用

    第3回宇宙メラトニン研究会  2013年 

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  • 疑似微小重力に対するキンギョ再生ウロコにおける破骨細胞の応答とRANKL発現変化

    第55回歯科医基礎医学会学術大会・総会  2013年 

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  • 過重力及び擬似微小重力に対する骨芽細胞及び破骨細胞の応答解析

    平成24年度 日本動物学会中部支部会 松本大会  2012年 

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  • LIPUSの骨芽細胞及び破骨細胞に対する作用:魚の培養ウロコを骨のモデルとした解析

    第15回超音波骨折治療研究会  2012年 

     詳細を見る

  • 魚類のウロコを用いた宇宙生物学的研究:新規メラトニン誘導体のウロコ及び骨疾患ラットの

    第28回宇宙利用シンポジウム  2012年 

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  • キンギョ・ウロコを使った宇宙実験:微小重力下における破骨細胞の超微細構造解析

    第117回日本解剖学会総会・全国学術集会  2012年 

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  • 宇宙(微小重力)における破骨細胞の亢進とメラトニンによる抑制効果――「きぼう」実験棟における研究成果

    第12回 日本抗加齢医学会総会  2012年 

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  • 宇宙空間におけるウロコの破骨細胞の形態及び細胞活性の変化

    平成24年度 日本動物学会中部支部会 松本大会  2012年 

     詳細を見る

  • in vivo electroporationを用いたBMP-2遺伝子導入による筋内異所性骨誘導における筋組織の変化

    第88回日本生理学会大会、第116回日本解剖学会総会・全国集会 合同大会  2011年 

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  • 微小重力に対する破骨細胞の応答:魚のウロコを用いた形態学的解析

    第31回 日本骨形態計測学会  2011年 

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  • キンギョのウロコに存在する破骨細胞は微小重力下で活性化する

    第29回 日本骨代謝学会学術集会  2011年 

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  • 宇宙におけるウロコの破骨細胞の形態及び細胞活性の変化

    日本動物学会中部支部大会  2011年 

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  • 骨とメラトニン

    第3回抗加齢内分泌研究会学術集会  2011年 

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  • 骨代謝に関する形態学的研究

    第72回生命科学先端研究センター 学術セミナー  2011年 

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  • 微小重力に対するウロコの破骨細胞の応答:国際宇宙ステーションにおける宇宙実験

    第53回歯科基礎医学会サテライトシンポジウム  2011年 

     詳細を見る

  • 魚類のウロコを用いた宇宙生物学的

    第27回宇宙利用シンポジウム  2011年 

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  • 宇宙実験のためのキンギョ再生鱗の培養法の開発と保冷・培養下での細胞動態の観察

    第115回 日本解剖学会・全国学術集会  2010年 

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  • BMP2によるマウス歯根膜細胞の骨芽細胞様分化の際に認められるendoglin依存的Smad-2リン酸化

    第28回 日本骨代謝学会学術集会  2010年 

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  • BMP-2/7ヘテロダイマー産生ベクターによる骨再生法

    第52回歯科基礎医学会学術大会  2010年 

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  • 魚類のウロコを用いた宇宙生物学的研究:宇宙実験に適したウロコの培養法の検討

    第25回 宇宙利用シンポジム  2009年 

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  • Actin cytoskeleton and β-catenin localization in osteogenic cells of mouse cranial suture under tensile stress.

    第2回 国際骨ミネラル学会・オーストラリア・ニュージーランド骨代謝学会合同会議  2009年 

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  • ウズラ骨髄骨における骨形成と骨吸収

    第114回 日本解剖学会総会・全国学術集会  2009年 

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  • 歯槽骨再生を目標として遺伝子導入法の試み

    第114回 日本解剖学会総会・全国学術集会  2009年 

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  • 擬似微小重力及び過重力下における骨代謝制御:培養ウロコを用いた解析

    第24回宇宙利用シンポジム  2008年 

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  • 伸展刺激による骨芽細胞の分化とアクチン細胞骨格の形態変化

    日本実験力学会2008年度年次講演会  2008年 

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  • 副甲状腺ホルモンのウロコの骨芽細胞及び破骨細胞に対する作用

    第79回日本動物学会  2008年 

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  • 再生鱗を用いた評価系の開発と加速度重力の解析

    宇宙生物科学会第22回大会  2008年 

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  • 新規ブロモメラトニン誘導体の卵巣摘出ラットおよび低カルシウム食ラットの骨代謝に及ぼす影響

    第26回日本骨代謝学会  2008年 

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  • 新規ブロモメラトニンは破骨細胞の活性を抑制し、骨芽細胞の活性を上げる:培養ウロコを用いた解析

    第26回日本骨代謝学会  2008年 

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  • 超音波の機械的刺激及び加速度重力の刺激に対する骨芽・破骨細胞の応答

    日本動物学会  2007年 

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  • 魚類のウロコを用いた宇宙生物学的研究

    第23回宇宙利用シンポジウム  2007年 

     詳細を見る

  • 自家移植ウロコにおける破骨細胞形成に関する形態学的研究

    宇宙フォーラムワーキンググループ  2007年 

     詳細を見る

  • Bisphosphonateに起因する顎骨壊死についての基礎的検討

    第26回日本骨形態計測学会  2006年 

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  • Bisphosphonate投与サル顎骨の骨細胞壊死に関する形態計測学的研究

    第48回歯科基礎医学会学術大会  2006年 

     詳細を見る

  • カルシトニンが破骨細胞のカルシトニン受容体に及ぼす作用

    第5回カルシトニン・副甲状腺ホルモン研究会  2005年 

     詳細を見る

  • Morphological changes in actin cytoskeleton induced by tensile stress in mouse cranial suture.

    2nd Joint Meeting of the European Calcified Tissue Society and the International Bone and Mineral Society  2005年 

     詳細を見る

  • プロラクチンはキンギョのウロコに存在する破骨細胞の活性を抑制する

    日本動物学会第76回大会  2005年 

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  • Tensile stress-induced morphological changes in actin fibers located in the mouse cranial suture.

    16th International congress of the IFAA (international Federation of Associations of Anatomists) and 109th Annual Meeting of Japanese Association of Anatomists  2004年 

     詳細を見る

  • 歯の再植後の歯髄内硬組織形成について

    H13・H15学術フロンティア推進事業合同研究集会  2004年 

     詳細を見る

  • Observation of microstructural changes in the molar alveolar bone of ovariectomized monkeys.

    111th Scientific Meeting of Japan Prosthodontic Society, 2nd Joint Meeting of JPS and KAP  2004年 

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  • エストロゲン欠乏と歯槽骨変化 ―卵巣摘出サルの犬歯部歯槽骨の解析―

    第24回日本骨形態計測学会  2004年 

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  • Estrogen deficiency-induced alveolar bone loss around monkey canine.

    1st Asian pacific congress of bone morphometry  2004年 

     詳細を見る

  • Relationship between microstructural changes of molar alveolar bone and lumbar bone mineral density in ovariectomized monkeys.

    1st Asian pacific congress of bone morphometry  2004年 

     詳細を見る

  • Effects of estrogen deficiency on monkey mandibular condyles, and its correlation with mechanical stress caused by mastication.

    1st Asian pacific congress of bone morphometry  2004年 

     詳細を見る

  • 卵巣摘出によるサル下顎頭の骨密度及び構造の変化と応力分布について

    第17回日本顎関節学会総会・学術大会  2004年 

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  • Immunocytochemical and histochemical study of hard tissue formation in the dental pulp after tooth replantation in rat molars.

    16th International congress of the IFAA (international Federation of Associations of Anatomists) and 109th Annual Meeting of Japanese Association of Anatomists  2004年 

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  • 顎骨動態へのCalcitonin及びAlendronateの影響

    第46回歯科基礎医学会学術大会  2004年 

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  • 伸展刺激による頭頂骨縫合部細胞のアクチン線維の形態変化

    日本解剖学会 第59回中国・四国地方会  2004年 

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  • ラット臼歯再植後の歯髄治癒過程における歯髄内硬組織形成メカニズムの検索

    平成16年度新潟歯学会第2回例会  2004年 

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  • Hard tissue formation in the dental pulp during the regeneration process after tooth replantation in rat molars.

    8th International Conference on Tooth Morphogenesis and Differentiation  2004年 

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  • メカニカルストレスが誘導する骨形成作用とPIASxβの機能解析

    第22回日本骨代謝学会学術集会  2004年 

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  • Alendronateとprostaglandin E receptor (EP4) agonistの顎骨粗鬆化に対する予防効果 ―加齢に伴う顎骨反応の変化に関する検討―

    第108回日本解剖学会総会・全国学術集会  2003年 

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  • カルシトニンの骨に及ぼす効果 ―形態学的検討―

    第3回カルシトニン・副甲状腺ホルモン研究会  2003年 

     詳細を見る

  • Possibility of screening for systemic osteoporosis using jaw bone data.

    1st Joing Meeting of the International Bone and Mineral Society and the Japanese Society for Bone and Mineral Research  2003年 

     詳細を見る

  • Effects of Alentronate and prostaglandin E receptor (EP4) agonist on ovariectomized monkey mandible.

    1st Joing Meeting of the International Bone and Mineral Society and the Japanese Society for Bone and Mineral Research  2003年 

     詳細を見る

  • GDF5 expression pattern of ligament/tendon cell in vivo and in vitro.

    1st Joing Meeting of the International Bone and Mineral Society and the Japanese Society for Bone and Mineral Research  2003年 

     詳細を見る

  • Alendronateは卵巣摘出後のサル顎骨粗鬆化を抑制するが、顎骨動態にも影響を 及ぼす

    第20回日本骨代謝学会  2002年 

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  • 卵巣摘出後のサル顎骨に及ぼすAlendronateの影響

    第22回日本骨形態計測学会  2002年 

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  • 卵巣摘出サルにおける顎骨骨密度と体幹骨密度との関連性に関する検討

    第22回日本骨形態計測学会  2002年 

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  • 張力刺激による骨形成促進機構の解明

    松本歯科大学歯学総合研究所セミナー  2002年 

     詳細を見る

  • The effects of estrogen deficiency on monkey mandibular condyles following ovariectomy.

    The 32nd annyal international Sun Valley hard tissue workshop  2002年 

     詳細を見る

  • Tensile stress-inducible α-adaptin C enhances endocytosis and osteoblasts differentiation in calvarial sutures.

    23th Annual Meeting of the American Society for Bone and Mineral Research  2002年 

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  • 張力刺激による骨芽細胞の分化促進過程におけるαアダプチンCの遺伝子発現と被覆小胞形成

    第44回歯科基礎医学会  2002年 

     詳細を見る

  • 顎骨粗鬆化に対するAlendronateとprostaglandin E receptor (EP4) agonist の影響

    第44回歯科基礎医学会  2002年 

     詳細を見る

  • 歯科における骨粗鬆症スクリーニングの基礎的検討

    平成14年度新潟歯学会2回例会  2002年 

     詳細を見る

  • in vivo, in vitroにおける靱帯・腱細胞のGDF5の発現状態についての検討

    平成14年度新潟歯学会2回例会  2002年 

     詳細を見る

  • In vivoにおける破骨細胞のカルシトニンへの反応性と血中カルシウム濃度

    カルシトニン・副甲状腺ホルモン研究会  2002年 

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  • Genes resposible for osteogenesis induced by mechanical stress in the mouse calvarial sutures in culture.

    The 8th International symposium on Biomineralization "Biomineralization: formation, diversity, evolution and application"  2001年 

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  • マウス頭頂骨縫合部に張力刺激を加えるとα-adaptin Cの発現が誘導される -増殖因子受容体のendocytosisが細胞分化への振り分けに関与する-

    第19回日本骨代謝学会  2001年 

     詳細を見る

  • In vivoにおける破骨細胞のカルシトニンレセプターのdownregulationおよび 回復に関する微細構造学的・ラジオオートグラフィー的研究

    第19回日本骨代謝学会  2001年 

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  • 張力刺激による骨形成促進過程の微細形態学的・細胞化学的解析

    第105回日本解剖学会総会・全国学術集会  2000年 

     詳細を見る

  • Tensile mechanical stress stimulated osteoblasts differentiation and bone formation in murine calvarial suture.

    The 105th Japanese Association of Anatomists  2000年 

     詳細を見る

  • 張力刺激により誘導される骨形成促進機構

    第12回日本理学診療医学会  2000年 

     詳細を見る

  • 張力刺激による頭頂骨縫合部の骨芽細胞分化促進過程の解析

    第18回日本骨代謝学会  2000年 

     詳細を見る

  • 張力刺激により形成促進される骨基質の形態学的解析

    第42回歯科基礎医学会  2000年 

     詳細を見る

  • 骨基質蛋白と遺伝子発現

    第8回バイオミネラリゼーション国際研究集会プレシンポジウム「硬組織・バイオミネラルのつくりと働き」  2000年 

     詳細を見る

  • The process of bone matrix formation stimulated by tensile mechanical stress.

    10th Annual Scientific Meeting of Australian and New Zealand Bone and Mineral Society, International Bone and Hormone Meeting  2000年 

     詳細を見る

  • 張力刺激によりマウス頭頂骨縫合部に誘導される遺伝子群の解析

    平成12年度新潟歯学会第2回例会  2000年 

     詳細を見る

  • 骨髄組織における間質系細胞ネットワークおよびその破骨細胞形成への関与: 組織化学的・微細構造学的研究

    平成12年度新潟歯学会第2回例会  2000年 

     詳細を見る

  • ラット臼歯の生理的遠心移動における有細胞セメント質のセメント細胞・セメント芽細胞のオステオポンチンの局在と発現

    平成12年度新潟歯学会第2回例会  2000年 

     詳細を見る

  • マウス歯根膜細胞株のクローニングとその形質:遺伝子発現の骨芽細胞との相違

    平成12年度新潟歯学会第2回例会  2000年 

     詳細を見る

  • Tensile stress markedly increased tetranectin gene expression in cranial sutures of mouse calvariae.

    21th Annual Meeting of the American Society for Bone and Mineral Research  1999年 

     詳細を見る

  • 破骨細胞の分化過程にはALP陽性の骨髄間質系細胞のネットワークが関与する

    第41回歯科基礎医学会  1999年 

     詳細を見る

  • Murine periodontal ligament cell lines: Isolation and characterization.

    21st Annual Meeting of the American Society for Bone and Mineral Research  1999年 

     詳細を見る

  • 張力刺激により惹起される骨形成促進機構の解析

    第41回歯科基礎医学会  1999年 

     詳細を見る

  • 張力刺激によりマウス頭頂骨縫合部に誘導される遺伝子群の解析

    第17回日本骨代謝学会  1999年 

     詳細を見る

  • 張力刺激により誘導される骨芽細胞の分化にはBMP-4遺伝子の発現が重要である

    第17回日本骨代謝学会  1999年 

     詳細を見る

  • マウス歯根膜細胞株のクローニングとその形質:遺伝子発現の骨芽細胞との相違

    第17回日本骨代謝学会  1999年 

     詳細を見る

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受賞

  • 優秀演題賞

    2016年  

     詳細を見る

    受賞国:日本国

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  • Zoological science award

    2014年  

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  • Fujii award

    2014年  

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  • 動物学会賞

    2014年  

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    受賞国:日本国

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  • Fujii award

    2014年  

     詳細を見る

    受賞国:日本国

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  • 優秀ポスター賞

    2014年  

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    受賞国:日本国

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  • 総合歯科医学2 (2020年度) 第2学期  - 月3,火3,水3,金1~2

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