Updated on 2024/02/07

写真a

 
TOYODA Kazuhiro
 
Organization
Faculty of Environmental, Life, Natural Science and Technology Professor
Position
Professor
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Degree

  • (BLANK) ( Okayama University )

Research Interests

  • 分子植物病理学

  • Molecular Plant Pathology

Research Areas

  • Environmental Science/Agriculture Science / Plant protection science

Education

  • Okayama University    

    - 1994

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  • Okayama University   自然科学研究科   生物開発科学

    - 1994

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    Country: Japan

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  • Mie University   農学部   農学

    - 1989

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    Country: Japan

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  • Mie University    

    - 1989

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Professional Memberships

  • 日本植物生理学会

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  • 日本植物病理学会

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  • 植物微生物研究会

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  • 米国植物生理学会(American Society of Plant Physiologists)

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  • 国際植物一微生物相互作用学会(International Society for Molecular Plant-Microbe Interactions)

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Papers

  • Rhizoviticin is an alphaproteobacterial tailocin that mediates biocontrol of grapevine crown gall disease

    Tomoya Ishii, Natsuki Tsuchida, Niarsi Merry Hemelda, Kirara Saito, Jiyuan Bao, Megumi Watanabe, Atsushi Toyoda, Takehiro Matsubara, Mayuko Sato, Kiminori Toyooka, Nobuaki Ishihama, Ken Shirasu, Hidenori Matsui, Kazuhiro Toyoda, Yuki Ichinose, Tetsuya Hayashi, Akira Kawaguchi, Yoshiteru Noutoshi

    The ISME Journal   18 ( 1 )   2024.1

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    Publishing type:Research paper (scientific journal)   Publisher:Oxford University Press (OUP)  

    Abstract

    Tailocins are headless phage tail structures that mediate interbacterial antagonism. Although the prototypical tailocins, R- and F-pyocins, in Pseudomonas aeruginosa, and other predominantly R-type tailocins have been studied, their presence in Alphaproteobacteria remains unexplored. Here, we report the first alphaproteobacterial F-type tailocin, named rhizoviticin, as a determinant of the biocontrol activity of Allorhizobium vitis VAR03-1 against crown gall. Rhizoviticin is encoded by a chimeric prophage genome, one providing transcriptional regulators and the other contributing to tail formation and cell lysis, but lacking head formation genes. The rhizoviticin genome retains a nearly intact early phage region containing an integrase remnant and replication-related genes critical for downstream gene transcription, suggesting an ongoing transition of this locus from a prophage to a tailocin-coding region. Rhizoviticin is responsible for the most antagonistic activity in VAR03-1 culture supernatant against pathogenic A. vitis strain, and rhizoviticin deficiency resulted in a significant reduction in the antitumorigenic activity in planta. We identified the rhizoviticin-coding locus in eight additional A. vitis strains from diverse geographical locations, highlighting a unique survival strategy of certain Rhizobiales bacteria in the rhizosphere. These findings advance our understanding of the evolutionary dynamics of tailocins and provide a scientific foundation for employing rhizoviticin-producing strains in plant disease control.

    DOI: 10.1093/ismejo/wrad003

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  • CEP ペプチドを介した植物免疫の制御機構 Invited

    豊田和弘, 白石友紀

    細胞   55 ( 9 )   78 - 82   2023.8

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    Authorship:Lead author   Language:Japanese   Publishing type:Research paper (scientific journal)  

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  • Positive chemotaxis to plant apoplastic fluids of Pseudomonas syringae pv. tabaci 6605 and metabolome analysis Reviewed

    Yuta Watanabe, Stephany Angelia Tumewu, Hajime Yamada, Hidenori Matsui, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda, Yuki Ichinose

    Journal of General Plant Pathology   89 ( 4 )   219 - 223   2023.6

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    Abstract

    Pseudomonas syringae pv. tabaci 6605 (Pta6605) is a causal agent of wildfire disease in host tobacco plants. Although chemotaxis has been shown to be necessary for Pta6605 in tobacco infection, the chemoattractants at the site of infection are unclear. Pta6605 was attracted to the apoplastic fluid from not only host tobacco leaves but also non-host plant leaves, indicating that Pta6605 is attracted to common plant metabolites. Metabolome analysis of apoplastic fluid from tobacco leaves revealed that amino acids including γ-aminobutyric acid and organic acids are abundant, suggesting that these compounds are potential chemoattractants.

    DOI: 10.1007/s10327-023-01126-4

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    Other Link: https://link.springer.com/article/10.1007/s10327-023-01126-4/fulltext.html

  • CEP peptide, a family of conserved, secreted small peptides acts as an endogenous suppressor in Arabidopsis Invited Reviewed

    Toyoda, K, Fitrianti, A.N, Itoh, C, Hasegawa, H, Matsui, H, Noutoshi, Y, Yamamoto, M, Ichinose, Y, Shiraishi, T

    125   102019   2023.4

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.pmpp.2023.102019

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  • Requirement of chemotaxis and aerotaxis in host tobacco infection by Pseudomonas syringae pv. tabaci 6605 Reviewed

    Yuki Ichinose, Yuta Watanabe, Stephany Angelia Tumewu, Hidenori Matsui, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda

    Physiological and Molecular Plant Pathology   124   101970 - 101970   2023.3

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    Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.pmpp.2023.101970

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  • Time-series transcriptome of Brachypodium distachyon during bacterial flagellin-induced pattern-triggered immunity

    Tsubasa Ogasahara, Yusuke Kouzai, Megumi Watanabe, Akihiro Takahashi, Kotaro Takahagi, June-Sik Kim, Hidenori Matsui, Mikihiro Yamamoto, Kazuhiro Toyoda, Yuki Ichinose, Keiichi Mochida, Yoshiteru Noutoshi

    Frontiers in Plant Science   13   2022.9

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    Publishing type:Research paper (scientific journal)   Publisher:Frontiers Media {SA}  

    <jats:p>Plants protect themselves from microorganisms by inducing pattern-triggered immunity (PTI) <jats:italic>via</jats:italic> recognizing microbe-associated molecular patterns (MAMPs), conserved across many microbes. Although the MAMP perception mechanism and initial events during PTI have been well-characterized, knowledge of the transcriptomic changes in plants, especially monocots, is limited during the intermediate and terminal stages of PTI. Here, we report a time-series high-resolution RNA-sequencing (RNA-seq) analysis during PTI in the leaf disks of <jats:italic>Brachypodium distachyon</jats:italic>. We identified 6,039 differentially expressed genes (DEGs) in leaves sampled at 0, 0.5, 1, 3, 6, and 12 hours after treatment (hat) with the bacterial flagellin peptide flg22. The k-means clustering method classified these DEGs into 10 clusters (6 upregulated and 4 downregulated). Based on the results, we selected 10 PTI marker genes in <jats:italic>B. distachyon</jats:italic>. Gene ontology (GO) analysis suggested a tradeoff between defense responses and photosynthesis during PTI. The data indicated the recovery of photosynthesis started at least at 12 hat. Over-representation analysis of transcription factor genes and cis-regulatory elements in DEG promoters implied the contribution of 12 WRKY transcription factors in plant defense at the early stage of PTI induction.</jats:p>

    DOI: 10.3389/fpls.2022.1004184

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  • CEP peptide induces susceptibility of Arabidopsis thaliana to non-adapted pathogens Reviewed

    Aprilia Nur Fitrianti, Thanh Luan Mai, Le Thi Phuong, Hiyori Monden, Norika Shiiba, Hidenori Matsui, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi, Kazuhiro Toyoda

    Journal of General Plant Pathology   88 ( 5 )   287 - 292   2022.9

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s10327-022-01077-2

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    Other Link: https://link.springer.com/article/10.1007/s10327-022-01077-2/fulltext.html

  • HopAZ1, a type III effector of Pseudomonas amygdali pv. tabaci , induces a hypersensitive response in tobacco wildfire‐resistant Nicotiana tabacum ‘N509’

    Sachi Kashihara, Takafumi Nishimura, Yoshiteru Noutoshi, Mikihiro Yamamoto, Kazuhiro Toyoda, Yuki Ichinose, Hidenori Matsui

    Molecular Plant Pathology   23 ( 6 )   885 - 894   2022.6

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    Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1111/mpp.13198

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    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/mpp.13198

  • Surveillance of Pathogenicity of Rhizoctonia solani Japanese Isolates with Varied Anastomosis Groups and Subgroups on Arabidopsis thaliana

    Mai Mohsen Ahmed Abdelghany, Maria Kurikawa, Megumi Watanabe, Hidenori Matsui, Mikihiro Yamamoto, Yuki Ichinose, Kazuhiro Toyoda, Yusuke Kouzai, Yoshiteru Noutoshi

    Life   12 ( 1 )   76 - 76   2022.1

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    Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    Rhizoctonia solani is a necrotrophic plant pathogen with a wide host range. R. solani is a species complex consisting of thirteen anastomosis groups (AGs) defined by compatibility of hyphal fusion reaction and subgroups based on cultural morphology. The relationship between such classifications and host specificity remains elusive. Here, we investigated the pathogenicity of seventeen R. solani isolates (AG-1 to 7) in Japan towards Arabidopsis thaliana using leaf and soil inoculations. The tested AGs, except AG-3 and AG-6, induced symptoms in both methods with variations in pathogenicity. The virulence levels differed even within the same AG and subgroup. Some isolates showed tissue-specific infection behavior. Thus, the AGs and their subgroups are suggested to be not enough to define the virulence (host and tissue specificity) of R. solani. We also evaluated the virulence of the isolates on Arabidopsis plants pretreated with salicylic acid, jasmonic acid, and ethylene. No obvious effects were detected on the symptom formation by the virulence isolates, but ethylene and salicylic acid slightly enhanced the susceptibility to the weak and nonvirulent isolates. R. solani seems to be able to overcome the induced defense by these phytohormones in the infection to Arabidopsis.

    DOI: 10.3390/life12010076

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  • Identification of Aerotaxis Receptor Proteins Involved in Host Plant Infection by Pseudomonas syringae pv. tabaci 6605.

    Stephany Angelia Tumewu, Yuta Watanabe, Hidenori Matsui, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda, Yuki Ichinose

    Microbes and environments   37 ( 1 )   2022

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    Language:English   Publishing type:Research paper (scientific journal)  

    Pseudomonas syringae pv. tabaci 6605 (Pta6605) is a foliar plant pathogen that causes wildfire disease on tobacco plants. It requires chemotaxis to enter plants and establish infection. While chemotactic signals appear to be the main mechanism by which Pta6605 performs directional movement, the involvement of aerotaxis or energy taxis by this foliar pathogen is currently unknown. Based on domain structures and similarity with more than 50 previously identified putative methyl-accepting chemotaxis proteins (MCPs), the genome of Pta6605 encodes three potential aerotaxis transducers. We identified AerA as the main aerotaxis transducer and found that it possesses a taxis-to-serine-and-repellent (Tsr)-like domain structure that supports a periplasmic 4HB-type ligand-binding domain (LBD). The secondary aerotaxis transducer, AerB, possesses a cytosolic PAS-type LBD, similar to the Aer of Escherichia coli and Pseudomonas aeruginosa. Aerotaxis ability by single and double mutant strains of aerA and aerB was weaker than that by wild-type Pta6605. On the other hand, another cytosolic PAS-type LBD containing MCP did not make a major contribution to Pta6605 aerotaxis in our assay system. Furthermore, mutations in aerotaxis transducer genes did not affect surface motility or chemotactic attraction to yeast extract. Single and double mutant strains of aerA and aerB showed less colonization in the early stage of host plant infection and lower biofilm production than wild-type Pta6605. These results demonstrate the presence of aerotaxis transducers and their contribution to host plant infection by Pta6605.

    DOI: 10.1264/jsme2.ME21076

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  • Role of Trehalose Synthesis in Ralstonia syzygii subsp. indonesiensis PW1001 in Inducing Hypersensitive Response on Eggplant (Solanum melongena cv. Senryo-nigou)

    Nur Laili, Takafumi Mukaihara, Hidenori Matsui, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda, Yuki Ichinose

    The Plant Pathology Journal   37 ( 6 )   566 - 579   2021.12

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    Publishing type:Research paper (scientific journal)   Publisher:Korean Society of Plant Pathology  

    Ralstonia syzygii subsp. indonesiensis (Rsi, former name: Ralstonia solanacearum phylotype IV) PW1001, a causal agent of potato wilt disease, induces hypersensitive response (HR) on its non-host eggplant (Solanum melongena cv. Senryo-nigou). The disaccharide trehalose is involved in abiotic and biotic stress tolerance in many organisms. We found that trehalose is required for eliciting HR on eggplant by plant pathogen Rsi PW1001. In R. solanacearum, it is known that the OtsA/OtsB pathway is the dominant trehalose synthesis pathway, and otsA and otsB encode trehalose-6-phosphate (T6P) synthase and T6P phosphatase, respectively. We generated otsA and otsB mutant strains and found that these mutant strains reduced the bacterial trehalose concentration and HR induction on eggplant leaves compared to wild-type. Trehalose functions intracellularly in Rsi PW1001 because addition of exogenous trehalose did not affect the HR level and ion leakage. Requirement of trehalose in HR induction is not common in R. solanacearum species complex because mutation of otsA in Ralstonia pseudosolanacearum (former name: Ralstonia solanacearum phylotype I) RS1002 did not affect HR on the leaves of its non-host tobacco and wild eggplant Solanum torvum. Further, we also found that each otsA and otsB mutant had reduced ability to grow in a medium containing NaCl and sucrose, indicating that trehalose also has an important role in osmotic stress tolerance.

    DOI: 10.5423/ppj.oa.06.2021.0087

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    Other Link: http://ppjonline.org/journal/view.php?doi=10.5423/PPJ.OA.06.2021.0087

  • Identification of chemoreceptor proteins for amino acids involved in host plant infection in Pseudomonas syringae pv. tabaci 6605

    Stephany Angelia Tumewu, Hidenori Matsui, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda, Yuki Ichinose

    Microbiological Research   253   126869 - 126869   2021.12

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    Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.micres.2021.126869

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  • Vfr targets promoter of genes encoding methyl-accepting chemotaxis protein in Pseudomonas syringae pv. tabaci 6605

    Keisuke Ogura, Hidenori Matsui, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda, Fumiko Taguchi, Yuki Ichinose

    Biochemistry and Biophysics Reports   26   100944 - 100944   2021.7

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    Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.bbrep.2021.100944

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  • Complete Genome Sequence of Pseudomonas amygdali pv. tabaci Strain 6605, a Causal Agent of Tobacco Wildfire Disease

    Hidenori Matsui, Takafumi Nishimura, Shuta Asai, Sachiko Masuda, Ken Shirasu, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda, Yuki Ichinose

    MICROBIOLOGY RESOURCE ANNOUNCEMENTS   10 ( 28 )   2021.7

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:AMER SOC MICROBIOLOGY  

    Pseudomonas amygdali pv. tabaci strain 6605 is the bacterial pathogen causing tobacco wildfire disease that has been used as a model for elucidating virulence mechanisms. Here, we present the complete genome sequence of P. amygdali pv. tabaci 6605 as a circular chromosome from reads using a PacBio sequencer.

    DOI: 10.1128/MRA.00405-21

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  • Cluster II che genes of Pseudomonas syringae pv. tabaci 6605, orthologs of cluster I in Pseudomonas aeruginosa, are required for chemotaxis and virulence

    Stephany Angelia Tumewu, Yujiro Ogawa, Takumi Okamoto, Yuka Sugihara, Hajime Yamada, Fumiko Taguchi, Hidenori Matsui, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda, Yuki Ichinose

    Molecular Genetics and Genomics   2021.1

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    Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s00438-020-01745-y

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    Other Link: http://link.springer.com/article/10.1007/s00438-020-01745-y/fulltext.html

  • HopH1 effectors of Pseudomonas syringae pv. tomato DC3000 and pv. syringae B728a induce HR cell death in nonhost eggplant Solanum torvum

    Kamrun Nahar, Takafumi Mukaihara, Fumiko Taguchi, Hidenori Matsui, Mikihiro Yamamoto, Kazuhiro Toyoda, Yoshiteru Noutoshi, Tomonori Shiraishi, Yuki Ichinose

    Journal of General Plant Pathology   87 ( 1 )   24 - 29   2021.1

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    Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s10327-020-00961-z

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    Other Link: http://link.springer.com/article/10.1007/s10327-020-00961-z/fulltext.html

  • Identification of effector candidate genes of Rhizoctonia solani AG-1 IA expressed during infection in Brachypodium distachyon

    Sobhy S. H. Abdelsalam, Yusuke Kouzai, Megumi Watanabe, Komaki Inoue, Hidenori Matsui, Mikihiro Yamamoto, Yuki Ichinose, Kazuhiro Toyoda, Seiji Tsuge, Keiichi Mochida, Yoshiteru Noutoshi

    Scientific Reports   10 ( 1 )   2020.12

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    Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    <title>Abstract</title>
    <italic>Rhizoctonia solani</italic> is a necrotrophic phytopathogen belonging to basidiomycetes. It causes rice sheath blight which inflicts serious damage in rice production. The infection strategy of this pathogen remains unclear. We previously demonstrated that salicylic acid-induced immunity could block <italic>R. solani</italic> AG-1 IA infection in both rice and <italic>Brachypodium distachyon</italic>. <italic>R. solani</italic> may undergo biotrophic process using effector proteins to suppress host immunity before necrotrophic stage. To identify pathogen genes expressed at the early infection process, here we developed an inoculation method using <italic>B. distachyon</italic> which enables to sample an increased amount of semi-synchronous infection hyphae. Sixty-one <italic>R. solani secretory effector-like protein</italic> genes (<italic>RsSEPGs</italic>) were identified using in silico approach with the publicly available gene annotation of <italic>R. solani</italic> AG-1 IA genome and our RNA-sequencing results obtained from hyphae grown on agar medium. Expression of <italic>RsSEPGs</italic> was analyzed at 6, 10, 16, 24, and 32 h after inoculation by a quantitative reverse transcription-polymerase chain reaction and 52 genes could be detected at least on a single time point tested. Their expressions showed phase-specific patterns which were classified into 6 clusters. The 23 <italic>RsSEPGs</italic> in the cluster 1–3 and 29 <italic>RsSEPGs</italic> in the cluster 4–6 are expected to be involved in biotrophic and necrotrophic interactions, respectively.

    DOI: 10.1038/s41598-020-71968-x

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    Other Link: http://www.nature.com/articles/s41598-020-71968-x

  • Role of Two Sets of RND-Type Multidrug Efflux Pump Transporter Genes, mexAB-oprM and mexEF-oprN, in Virulence of Pseudomonas syringae pv. tabaci 6605

    Yuki Ichinose, Takafumi Nishimura, Minori Harada, Ryota Kashiwagi, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda, Fumiko Taguchi, Daigo Takemoto, Hidenori Matsui

    PLANT PATHOLOGY JOURNAL   36 ( 2 )   148 - 156   2020.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:KOREAN SOC PLANT PATHOLOGY  

    Pseudomonas syringae pv. tabaci 6605 has two multidrug resistance (MDR) efflux pump transporters, MexAB-OprM and MexEF-OprN. To understand the role of these MDR efflux pumps in virulence, we generated deletion mutants, Delta mexB, Delta mexF, and Delta mexB Delta mexF, and investigated their sensitivity to plant-derived antimicrobial compounds, antibiotics, and virulence. Growth inhibition assays with KB soft agar plate showed that growth of the wild-type (WT) was inhibited by 5 mu l of 1 M catechol and 1 M coumarin but not by other plant-derived potential antimicrobial compounds tested including phytoalexins. The sensitivity to these compounds tended to increase in Delta mexB and Delta mexB Delta mexF mutants. The Delta mexB Delta mexF mutant was also sensitive to 2 M acetovanillone. The mexAB-oprM was constitutively expressed, and activated in the Delta mexF and Delta mexB Delta mexF mutant strains. The swarming and swimming motilities were impaired in Delta mexF and Delta mexB Delta rnexF mutants. The flood inoculation test indicated that bacterial populations in all mutant strains were significantly lower than that of WT, although all mutants and WT caused similar disease symptoms. These results indicate that MexAB-OprM extrudes plant-derived catechol, acetovanillone, or coumarin, and contributes to bacterial virulence. Furthermore, MexAB-OprM and MexEF-OprN complemented each other's functions to some extent.

    DOI: 10.5423/PPJ.OA.11.2019.0273

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  • Antagonism between SA- and JA-signaling conditioned by saccharin in Arabidopsis thaliana renders resistance to a specific pathogen

    Le Thi Phuong, Aprilia Nur Fitrianti, Mai Thanh Luan, Hidenori Matsui, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi, Kazuhiro Toyoda

    JOURNAL OF GENERAL PLANT PATHOLOGY   86 ( 2 )   86 - 99   2020.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER JAPAN KK  

    Saccharin is generated from probenazole (PBZ) in plants and acts as a plant defense activator. Our study of the mechanism underlying saccharin-induced systemic acquired resistance in Arabidopsis thaliana suggests an antagonistic interaction between salicylic acid (SA)- and jasmonic acid (JA)-signaling as revealed through gene expression analyses. In wild-type plants (Col-0) exposed to saccharin, there was a consistent increase in callose deposition and in expression of SA-marker genes, PR1 and PR2, which coincided with a decrease in expression of JA-marker genes such as VSP2, LOX2 and PDF1.2. Actually, pretreatment of Col-0 with saccharin or PBZ conferred resistance to Pseudomonas syringae pv. tomato DC3000, but not to Pectobacterium carotovorum subsp. carotovorum, Botrytis cinerea, or Colletotrichum higginsianum. Enhanced expression of SA- and JA-marker genes and the augmented deposition of callose were evident after a challenge with virulent DC3000 in saccharin-pretreated plants. Consistently, pretreatment of saccharin and PBZ with SA- and JA-defective mutants led to diminished resistance in NahG-transgenic and npr1 mutant plants, but not in jar1 mutant plants, suggesting that saccharin and PBZ induce resistance in A. thaliana against Pto DC3000 mainly via activation of SA-signaling, leading to suppression of JA/ET-signaling and vice versa. Collectively, an antagonism between SA- and JA-signaling conditioned by saccharin renders resistance to a specific pathogen in Arabidopsis.

    DOI: 10.1007/s10327-019-00899-x

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  • PsyR, a transcriptional regulator in quorum sensing system, binds lux box-like sequence in psyI promoter without AHL quorum sensing molecule and activates psyI transcription with AHL in Pseudomonas syringae pv. tabaci 6605

    Yuki Ichinose, Yousuke Tasaka, Satoru Yamamoto, Yuko Inoue, Motohiro Takata, Yukiko Nakatsu, Fumiko Taguchi, Mikihiro Yamamoto, Kazuhiro Toyoda, Yoshiteru Noutoshi, Hidenori Matsui

    JOURNAL OF GENERAL PLANT PATHOLOGY   86 ( 2 )   124 - 133   2020.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER JAPAN KK  

    Quorum sensing (QS) is a mechanism for bacterial cell-cell communication using QS signals. N-acyl-homoserine lactones (AHLs), QS signals in Pseudomonas syringae pv. tabaci (Pta) 6605, are synthesized by an AHL synthase (PsyI) and recognized by the cognate transcription factor PsyR. To reveal the role of PsyR in virulence, we generated a psyR mutant and complemented strains of Pta 6605 and found that the psyR mutant is remarkably reduced in AHL production and ability to cause disease and propagate in host tobacco leaves. The phenotypes of complemented strains were restored to that of the wild type (WT). Because the psyR mutant lost nearly all AHL production, we investigated the function of PsyR in the transcription of psyI and production of AHL. Electrophoretic mobility shift assays suggested that the recombinant PsyR protein binds the promoter region of psyI but not psyR without AHL. The addition of AHL did not significantly affect this binding. The binding core sequence of this region was identified as a 20-bp lux box-like sequence. To reveal the function of PsyR and AHL on psyI transcription, we constructed a psyI promoter::lacZYA chimeric reporter gene, and inserted it into the WT and psyI mutant of Pta 6605. beta-galactosidase activity increased in a bacterial density-dependent manner in the WT and also in a psyI mutant after the addition of exogenous AHL. These results indicate that the solo PsyR binds the lux box in the psyI promoter and activates transcription in the concomitant presence of AHL.

    DOI: 10.1007/s10327-019-00893-3

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  • The plant activator saccharin induces resistance to wheat powdery mildew by activating multiple defense-related genes

    Le Thi Phuong, Lei Zhao, Aprilia Nur Fitrianti, Hidenori Matsui, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi, Kazuhiro Toyoda

    JOURNAL OF GENERAL PLANT PATHOLOGY   86 ( 2 )   107 - 113   2020.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER JAPAN KK  

    Saccharin and its parental compound probenazole (PBZ) are plant activators of effective defense responses to (hemi) biotrophic pathogens. Here, we demonstrate that pretreatment of wheat seedlings with saccharin or PBZ results in a significant reduction of powdery mildew caused by Blumeria graminis f. sp. tritici. Transcriptional analysis revealed the expression of 15 defense-related genes including PR genes, WCI genes, LOX, AOS, NPR1, PAL and WRKY genes in wheat seedlings exposed to either saccharin or PBZ. Moreover, the saccharin- and PBZ-enhanced expression of those genes during fungal infection further proved a close correlation with increased resistance in wheat.

    DOI: 10.1007/s10327-019-00900-7

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  • Endogenous suppressor(s) in Arabidopsis thaliana

    Thanh Luan Mai, Tatsuhiro Kawasaki, Aprilia Nur Fitrianti, Le Thi Phuong, Tsugumi Shiokawa, Hiroko Tada, Hidenori Matsui, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi, Kazuhiro Toyoda

    JOURNAL OF GENERAL PLANT PATHOLOGY   86 ( 2 )   100 - 106   2020.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER JAPAN KK  

    An ethyl acetate extract of Arabidopsis thaliana plants was tested for the presence of endogenous suppressor(s) (ES), and the active fraction, which partitioned into water phase contained a molecule(s) < 3000 Da based on a rough estimate using sized membrane filters. Foliar application of the ES enabled typically nonpathogenic fungi (non-adapted pathogens) to cause disease symptoms on A. thaliana. Consistently, the ES fraction severely suppressed the oxidative burst and the expression of defense-related genes such as FRK1, NHO1, WRKY22, WRKY29, PEN2, and PEN3 in plants challenged with non-adapted fungus Colletotrichum gloeosporioides or the fungal elicitor chitin.

    DOI: 10.1007/s10327-019-00897-z

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  • Requirement of γ-Aminobutyric Acid Chemotaxis for Virulence of Pseudomonas syringae pv. tabaci 6605.

    Stephany Angelia Tumewu, Hidenori Matsui, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda, Yuki Ichinose

    Microbes and environments   35 ( 4 )   2020

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    γ-Aminobutyric acid (GABA) is a widely distributed non-proteinogenic amino acid that accumulates in plants under biotic and abiotic stress conditions. Recent studies suggested that GABA also functions as an intracellular signaling molecule in plants and in signals mediating interactions between plants and phytopathogenic bacteria. However, the molecular mechanisms underlying GABA responses to bacterial pathogens remain unknown. In the present study, a GABA receptor, named McpG, was conserved in the highly motile plant-pathogenic bacteria Pseudomonas syringae pv. tabaci 6605 (Pta6605). We generated a deletion mutant of McpG to further investigate its involvement in GABA chemotaxis using quantitative capillary and qualitative plate assays. The wild-type strain of Pta6605 was attracted to GABA, while the ΔmcpG mutant abolished chemotaxis to 10‍ ‍mM GABA. However, ΔmcpG retained chemotaxis to proteinogenic amino acids and succinic semialdehyde, a structural analog of GABA. Furthermore, ΔmcpG was unable to effectively induce disease on host tobacco plants in three plant inoculation assays: flood, dip, and infiltration inoculations. These results revealed that the GABA sensing of Pta6605 is important for the interaction of Pta6605 with its host tobacco plant.

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  • A class III peroxidase PRX34 is a component of disease resistance in Arabidopsis

    Lei Zhao, Le Thi Phuong, Mai Thanh Luan, Aprilia Nur Fitrianti, Hidenori Matsui, Hirofumi Nakagami, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi, Kazuhiro Toyoda

    JOURNAL OF GENERAL PLANT PATHOLOGY   85 ( 6 )   405 - 412   2019.11

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    PRX34 mediates the oxidative burst in Arabidopsis. Here we characterized two additional Arabidopsis prx34 null mutants (prx34-2, prx34-3), besides the well-studied prx34-1. Due to a decrease in corresponding peroxidase, the activity that generates reactive oxygen species (ROS) was significantly lower in cell wall extracts of prx34-2 and prx34-3 plants. Consistently, the prx34-2 and prx34-3 exhibited reduced accumulation both of ROS and callose in Flg22-elicitor-treated leaves, leading to enhanced susceptibility to bacterial and fungal pathogens. In contrast, ectopic expression of PRX34 in the wild type caused enhanced resistance. PRX34 is thus a component for disease resistance in Arabidopsis.

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  • Specific growth inhibitors of Ralstonia solanacearum, Xanthomonas oryzae pv. oryzae, X. campestris pv. campestris, and Clavibacter michiganensis subsp. michiganensis. Reviewed

    Ombiro GS, Sawai T, Noutoshi Y, Nishina Y, Matsui H, Yamamoto M, Toyoda K, Ichinose Y

    Microbiological research   215   29 - 35   2018.10

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    Plant pathogenic bacteria cause huge yield losses in crops globally. Therefore, finding effective bactericides to these pathogens is an immediate challenge. In this study, we sought compounds that specifically inhibit the growth of Ralstonia solanacearwn. As a result, we identified one promising compound, 1-(4-bromopheny1)-6-methoxy-2,3,4,9-tetrahydro-1H-beta-carboline, which inhibited the growth of R. solanacearum (Rs1002) from a pilot library of 376 chemicals provided from RIKEN. We further obtained its structural analogues and assessed their ability to inhibit Rs1002 growth. Then we identified five compounds, named ralhibitins A to E, that specifically inhibit growth of Rs1002 at >5 mu g/mI final concentration. The most effective compounds, ralhibitins A, C, and E completely inhibited the growth of Rs1002 at 1.25 mu g/ml. In addition, ralhibitins A to E inhibited growth of Xanthomonas oryzae pv. oryzae but not the other bacteria tested at a final concentration of 10 mu g/ml. Whereas, ralhibitin E, besides inhibiting R. solanacearum and X. oryzae pv. oryzae, completely inhibited the growth of X. campestris pv. campestris and the Gram-positive bacterium Clavibacter michiganensis subsp. michiganensis at 10 mu g/ml. Growth inhibition by these compounds was stable at pH 6-9 and after autoclaving. Because Rs1002 grew in the culture medium in which ralhibitins were incubated with the ralhibitin-insensitive bacteria, the unaffected bacteria may be able to inactivate the inhibitory effect of ralhibitins. These results suggest that ralhibitins might be potential lead compounds for the specific control of phytopathogenic bacteria.

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  • MexEF-OprN multidrug efflux pump transporter negatively controls N-acyl-homoserine lactone accumulation in pseudomonas syringae pv. Tabaci 6605. Reviewed

    Sawada T, Eguchi M, Asaki S, Kashiwagi R, Shimomura K, Taguchi F, Matsui H, Yamamoto M, Noutoshi Y, Toyoda K, Ichinose Y

    Molecular genetics and genomics : MGG   293 ( 4 )   907 - 917   2018.8

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    Our previous studies revealed that flagellar-motility-defective mutants such as a dagger fliC of Pseudomonas syringae pv. tabaci 6605 (Pta6605) have remarkably reduced production of N-acyl-homoserine lactones (AHL), quorum-sensing molecules. To investigate the reason of loss of AHL production in a dagger fliC mutant, we carried out transposon mutagenesis. Among approximately 14,000 transconjugants, we found 11 AHL production-recovered (APR) strains. In these APR strains, a transposon was inserted into either mexE or mexF, genes encoding for the multidrug efflux pump transporter MexEF-OprN, and mexT, a gene encoding a putative transcriptional activator for mexEF-oprN. These results suggest that MexEF-OprN is a negative regulator of AHL production. To confirm the negative effect of MexEF-OprN on AHL production, loss- and gain-of-function experiments for mexEF-oprN were carried out. The a dagger fliCa dagger mexF and a dagger fliCa dagger mexT double mutant strains recovered AHL production, whereas the mexT overexpressing strain abolished AHL production, although the psyI, a gene encoding AHL synthase, is transcribed as wild type. Introduction of a mexF or mexT mutation into another flagellar-motility- and AHL production-defective mutant strain, a dagger motCD, also recovered the ability to produce AHL. Furthermore, introduction of the mexF mutation into other AHL production-defective mutant strains such as a dagger gacA and a dagger aefR also recovered AHL production but not to the a dagger psyI mutant. These results indicate that MexEF-OprN is a decisive negative determinant of AHL production and accumulation.

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  • Salicylic acid-dependent immunity contributes to resistance against Rhizoctonia solani, a necrotrophic fungal agent of sheath blight, in rice and Brachypodium distachyon. Reviewed

    Yusuke Kouzai, Mamiko Kimura, Megumi Watanabe, Kazuki Kusunoki, Daiki Osaka, Tomoko Suzuki, Hidenori Matsui, Mikihiro Yamamoto, Yuki Ichinose, Kazuhiro Toyoda, Takakazu Matsuura, Izumi C. Mori, Takashi Hirayama, Eiichi Miami, Yoko Nishizawa, Komaki Inoue, Yoshihiko Onda, Keiichi Mochida, Yoshiteru Noutoshi

    New Phytologist   217 ( 2 )   771 - 783   2018

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    Rhizoctonia solani is a soil-borne fungus causing sheath blight. In consistent with its necrotrophic life style, no rice cultivars fully resistant to R. solani are known, and agrochemical plant defense activators used for rice blast, which upregulate a phytohormonal salicylic acid (SA)-dependent pathway, are ineffective towards this pathogen. As a result of the unavailability of genetics, the infection process of R. solani remains unclear.We used the model monocotyledonous plants Brachypodium distachyon and rice, and evaluated the effects of phytohormone-induced resistance to R. solani by pharmacological, genetic and microscopic approaches to understand fungal pathogenicity.Pretreatment with SA, but not with plant defense activators used in agriculture, can unexpectedly induce sheath blight resistance in plants. SA treatment inhibits the advancement of R. solani to the point in the infection process in which fungal biomass shows remarkable expansion and specific infection machinery is developed. The involvement of SA in R. solani resistance is demonstrated by SA-deficient NahG transgenic rice and the sheath blight-resistant B. distachyon accessions, Bd3-1 and Gaz-4, which activate SA-dependent signaling on inoculation.Our findings suggest a hemi-biotrophic nature of R. solani, which can be targeted by SA-dependent plant immunity. Furthermore, B. distachyon provides a genetic resource that can confer disease resistance against R. solani to plants.

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  • Characterization of the suppressive effects of the biological control strain VAR03-1 of Rhizobium vitis on the virulence of tumorigenic R-vitis Reviewed

    Kirara Saito, Megumi Watanabe, Hidenori Matsui, Mikihiro Yamamoto, Yuki Ichinose, Kazuhiro Toyoda, Akira Kawaguchi, Yoshiteru Noutoshi

    JOURNAL OF GENERAL PLANT PATHOLOGY   84 ( 1 )   58 - 64   2018

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    Rhizobium vitis: strain VAR03-1 is a biological control agent that suppresses grapevine crown gall disease caused by a tumorigenic strain of R. vitis (Ti). Both acetosyringone-induced expression of a virulence gene and the growth of Ti were suppressed in vitro when it was cultivated in the VAR03-1 culture filtrate. These inhibitory effects were reduced by high-temperature treatment or incubation for 72 h. Both activities were detected in the high molecular weight fraction (> 100 kDa) of the filtrate. Our results suggest that the antagonistic effects of VAR03-1 on Ti are mediated by large particle(s) released in the culture media.

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  • Ultrastructural and Cytological Studies on Mycosphaerella pinodes Infection of the Model Legume Medicago truncatula Reviewed

    Tomoko Suzuki, Aya Maeda, Masaya Hirose, Yuki Ichinose, Tomonori Shiraishi, Kazuhiro Toyoda

    FRONTIERS IN PLANT SCIENCE   8   2017.6

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    Ascochyta (Mycosphaerella) blight on cultivated peas is primarily caused by infection through asexual spores (pycnospores) of Mycosphaerella pinodes (Berk. et Blox.) Vestergren [recently renamed Peyronellaea pinodes (Berk. & A. Bloxam) Aveskamp, Gruyter & Verkley]. Using a model pathosystem involving Medicago truncatula and Mycosphaerella pinodes strain OMP-1, we examined the histology and ultrastructure of early infection events and fungal development including penetration by appressoria, vegetative growth of infection hyphae, and host responses. On the susceptible ecotype R108-1, pycnospores germinated and grew over the surface of the epidermis, then formed an appressoria and penetrated the cuticle. Beneath the cuticle, the infection peg expanded into a hyphae that grew within the outer wall of the epidermis. Subsequently, the hyphae penetrated down within mesophyll cells and proliferated vigorously, eventually, forming asexual fruiting bodies (pycnidia). In contrast, successful penetration and subsequent growth of infection hyphae were considerably restricted in the ecotype Caliph. Detected by its reaction with cerium chloride (CeCl3) to generate electron-dense cerium perhydroxides in transmission electron micrographs, hydrogen peroxide (H2O2) accumulated in epidermal and mesophyll cells of Caliph challenged with pycnospores of M. pinodes. This intracellular localization was confirmed by energydispersive X-ray spectroscopy. Our observations thus indicate that the oxidative burst reaction leading to the generation of reactive oxygen species is associated with a local host defense response in Caliph, since no clear H2O2 accumulation was detectable in susceptible R108-1. Indeed, aberrant hyphae such as intrahyphal hyphae and dead hyphae, probably due to a local defense elicited by the fungus, were abundant in Caliph but not in R108-1. Our results on the cellular interactions between the fungus and host cells provide additional insights to understand foliar infection by M. pinodes on cultivated peas.

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  • High Voltage Power Generation on Spacecraft

    Toyoda Kazuhiro

    IEEJ Transactions on Power and Energy   137 ( 11 )   698 - 701   2017

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    <p>The spacecrafts need high-voltage power generation for high power. However, the high voltage can cause severe discharge problem on spacecrafts. This paper describes the mechanism of spacecraft charging and discharging. The mitigation methods are also mentioned.</p>

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  • Reprint of “Sequence and phylogenetic analyses of novel totivirus-like double-stranded RNAs from field-collected powdery mildew fungi” Reviewed

    Hideki Kondo, Sakae Hisano, Sotaro Chiba, Kazuyuki Maruyama, Ida Bagus Andika, Kazuhiro Toyoda, Fumihiro Fujimori, Nobuhiro Suzuki

    Virus Research   219   39 - 50   2016.7

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    The identification of mycoviruses contributes greatly to understanding of the diversity and evolutionary aspects of viruses. Powdery mildew fungi are important and widely studied obligate phytopathogenic agents, but there has been no report on mycoviruses infecting these fungi. In this study, we used a deep sequencing approach to analyze the double-stranded RNA (dsRNA) segments isolated from field-collected samples of powdery mildew fungus-infected red clover plants in Japan. Database searches identified the presence of at least ten totivirus (genus Totivirus)-like sequences, termed red clover powdery mildew-associated totiviruses (RPaTVs). The majority of these sequences shared moderate amino acid sequence identity with each other (&lt
    44%) and with other known totiviruses (&lt
    59%). Nine of these identified sequences (RPaTV1a, 1b and 2–8) resembled the genome of the prototype totivirus, Saccharomyces cerevisiae virus-L-A (ScV-L-A) in that they contained two overlapping open reading frames (ORFs) encoding a putative coat protein (CP) and an RNA dependent RNA polymerase (RdRp), while one sequence (RPaTV9) showed similarity to another totivirus, Ustilago maydis virus H1 (UmV-H1) that encodes a single polyprotein (CP-RdRp fusion). Similar to yeast totiviruses, each ScV-L-A-like RPaTV contains a –1 ribosomal frameshift site downstream of a predicted pseudoknot structure in the overlapping region of these ORFs, suggesting that the RdRp is translated as a CP-RdRp fusion. Moreover, several ScV-L-A-like sequences were also found by searches of the transcriptome shotgun assembly (TSA) libraries from rust fungi, plants and insects. Phylogenetic analyses show that nine ScV-L-A-like RPaTVs along with ScV-L-A-like sequences derived from TSA libraries are clustered with most established members of the genus Totivirus, while one RPaTV forms a new distinct clade with UmV-H1, possibly establishing an additional genus in the family. Taken together, our results indicate the presence of diverse, novel totiviruses in the powdery mildew fungus populations infecting red clover plants in the field.

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  • Expression profiling of marker genes responsive to the defence-associated phytohormones salicylic acid, jasmonic acid and ethylene in Brachypodium distachyon Reviewed

    Yusuke Kouzai, Mamiko Kimura, Yurie Yamanaka, Megumi Watanabe, Hidenori Matsui, Mikihiro Yamamoto, Yuki Ichinose, Kazuhiro Toyoda, Yoshihiko Onda, Keiichi Mochida, Yoshiteru Noutoshi

    BMC PLANT BIOLOGY   16 ( 1 )   59   2016.3

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    Background: Brachypodium distachyon is a promising model plants for grasses. Infections of Brachypodium by various pathogens that severely impair crop production have been reported, and the species accordingly provides an alternative platform for investigating molecular mechanisms of pathogen virulence and plant disease resistance. To date, we have a broad picture of plant immunity only in Arabidopsis and rice; therefore, Brachypodium may constitute a counterpart that displays the commonality and uniqueness of defence systems among plant species. Phytohormones play key roles in plant biotic stress responses, and hormone-responsive genes are used to qualitatively and quantitatively evaluate disease resistance responses during pathogen infection. For these purposes, defence-related phytohormone marker genes expressed at time points suitable for defence-response monitoring are needed. Information about their expression profiles over time as well as their response specificity is also helpful. However, useful marker genes are still rare in Brachypodium.
    Results: We selected 34 candidates for Brachypodium marker genes on the basis of protein-sequence similarity to known marker genes used in Arabidopsis and rice. Brachypodium plants were treated with the defence-related phytohormones salicylic acid, jasmonic acid and ethylene, and their transcription levels were measured 24 and 48 h after treatment. Two genes for salicylic acid, 7 for jasmonic acid and 2 for ethylene were significantly induced at either or both time points. We then focused on 11 genes encoding pathogenesis-related (PR) 1 protein and compared their expression patterns with those of Arabidopsis and rice. Phylogenetic analysis suggested that Brachypodium contains several PR1-family genes similar to rice genes. Our expression profiling revealed that regulation patterns of some PR1 genes as well as of markers identified for defence-related phytohormones are closely related to those in rice.
    Conclusion: We propose that the Brachypodium immune hormone marker genes identified in this study will be useful to plant pathologists who use Brachypodium as a model pathosystem, because the timing of their transcriptional activation matches that of the disease resistance response. Our results using Brachypodium also suggest that monocots share a characteristic immune system, defined as the common defence system, that is different from that of dicots.

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  • Sequence and phylogenetic analyses of novel totivirus-like double-stranded RNAs from field-collected powdery mildew fungi Reviewed

    Hideki Kondo, Sakae Hisano, Sotaro Chiba, Kazuyuki Maruyama, Ida Bagus Andika, Kazuhiro Toyoda, Fumihiro Fujimori, Nobuhiro Suzuki

    VIRUS RESEARCH   213   353 - 364   2016.2

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    The identification of mycoviruses contributes greatly to understanding of the diversity and evolutionary aspects of viruses. Powdery mildew fungi are important and widely studied obligate phytopathogenic agents, but there has been no report on mycoviruses infecting these fungi. In this study, we used a deep sequencing approach to analyze the double-stranded RNA (dsRNA) segments isolated from field collected samples of powdery mildew fungus-infected red clover plants in Japan. Database searches identified the presence of at least ten totivirus (genus Totivirus)-like sequences, termed red clover powdery mildew-associated totiviruses (RPaTVs). The majority of these sequences shared moderate amino acid sequence identity with each other (&lt;44%) and with other known totiviruses (&lt;59%). Nine of these identified sequences (RPaTV1a, 1b and 2-8) resembled the genome of the prototype totivirus, Saccharomyces cerevisiae virus-L-A (ScV-L-A) in that they contained two overlapping open reading frames (ORFs) encoding a putative coat protein (CP) and an RNA dependent RNA polymerase (RdRp), while one sequence (RPaTV9) showed similarity to another totivirus, Ustilago maydis virus H1 (UmV-H1) that encodes a single polyprotein (CP-RdRp fusion). Similar to yeast totiviruses, each ScV-L-A-like RPaTV contains a-1 ribosomal frameshift site downstream of a predicted pseudoknot structure in the overlapping region of these ORFs, suggesting that the RdRp is translated as a CP-RdRp fusion. Moreover, several ScV-L-A-like sequences were also found by searches of the transcriptome shotgun assembly (TSA) libraries from rust fungi, plants and insects. Phylogenetic analyses show that nine ScV-L-A-like RPaTVs along with ScV-L-A-like sequences derived from TSA libraries are clustered with most established members of the genus Totivirus, while one RPaTV forms a new distinct Glade with UmV-H1, possibly establishing an additional genus in the family. Taken together, our results indicate the presence of diverse, novel totiviruses in the powdery mildew fungus populations infecting red clover plants in the field. (C) 2015 Elsevier B.V. All rights reserved.

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  • Leucine zipper motif in RRS1 is crucial for the regulation of Arabidopsis dual resistance protein complex RPS4/RRS1 Reviewed

    Narusaka Mari, Toyoda Kazuhiro, Shiraishi Tomonori, Iuchi Satoshi, Takano Yoshitaka, Shirasu Ken, Narusaka Yoshihiro

    SCIENTIFIC REPORTS   6   2016.1

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  • The plant cell wall as a site for molecular contacts in fungal pathogenesis Invited Reviewed

    Kazuhiro Toyoda, Sachiyo Yao, Mai Takagi, Maki Uchioki, Momiji Miki, Kaori Tanaka, Tomoko Suzuki, Masashi Amano, Akinori Kiba, Toshiaki Kato, Hirotaka Takahashi, Yasuhiro Ishiga, Hidenori Matsui, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi

    PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY   95   44 - 49   2016

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    The plant cell wall, the most external layer of the plant surface, is the site where most pathogenic fungi first make contact with host cells. A plant-fungus interaction therefore commences at the interface between the plant and the spore. Our current research focusing on the plant cell wall has discovered an extracellular ecto-nucleoside triphosphate diphosphohydrolase (ecto-NTPDase/apyrase; EC3.6.1.15) as a key player in plant defense before the onset of PTI (PAMP-triggered immunity). This review focuses on our recent findings, especially the role of the plant cell wall in the extracellular defense against fungi as well as fungal strategies resulting in successful infection. (C) 2016 Elsevier Ltd. All rights reserved.

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  • Motility-mediated regulation of virulence in Pseudomonas syringae Invited Reviewed

    Yuki Ichinose, Takahiro Sawada, Hidenori Matsui, Mikihiro Yamamoto, Kazuhiro Toyoda, Yoshiteru Noutoshi, Fumiko Taguchi

    PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY   95   50 - 54   2016

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    We have investigated Pseudomonas syringae pv. tabaci-plant interactions using a large variety of virulence-related mutants. A flagellin-defective mutant, Delta fliC, lost flagellar motility and the ability to produce N-acyl homoserine lactones; it had reduced ability to cause disease symptoms, but the expression of genes encoding a multidrug efflux pump transporter, mexEFoprN, was activated. A type IV pili (T4P)-defective mutant, Delta pilA, lost swarming motility, had reduced expression of hrp-related genes and virulence toward the host tobacco plant, but expression of the genes encoding another multidrug efflux pump transporter, mexABoprM, was activated. These results suggest that the genes regulating flagella- and T4P-mediated motilities also regulate expression of other virulence-related genes. (C) 2016 Elsevier Ltd. All rights reserved.

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  • Protection induced by volatile limonene against anthracnose disease in Arabidopsis thaliana Reviewed

    Fujioka K, Gotoh H, Noumi T, Yoshida A, Noutoshi Y, Inagaki Y, Yamamoto M, Ichinose Y, Shiraishi T, Toyoda K

    Journal of General Plant Pathology   81 ( 6 )   415 - 419   2015.9

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  • Characterization of quorum sensing-controlled transcriptional regulator MarR and Rieske (2Fe-2S) cluster-containing protein (Orf5), which are involved in resistance to environmental stresses in Pseudomonas syringae pv. tabaci 6605 Reviewed International journal

    Taguchi F, Inoue Y, Suzuki T, Inagaki Y, Yamamoto M, Toyoda K, Noutoshi Y, Shiraishi T, Ichinose Y

    Molecular Plant Pathology   16 ( 4 )   376 - 387   2014.8

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    Pseudomonas syringae pv. tabaci 6605 (Pta6605) produces acyl homoserine lactones (AHLs), quorum sensing (QS) molecules that are indispensable for virulence in host tobacco infection. Genome-wide transcriptional profiling of several QS-defective mutants revealed that the expression of the genes encoding the MarR family transcriptional regulator (MarR) and a Rieske 2Fe-2S cluster-containing protein (Orf5) located adjacent to psyI, a gene encoding AHL synthetase, are significantly repressed. Exogenous application of AHL recovered the expression of both marR and orf5 genes in the ΔpsyI mutant, indicating that AHL positively regulates the expression of these genes. To investigate the role of these genes in the virulence of Pta6605, ΔmarR and Δorf5 mutants were generated. Both mutants showed decreased swimming and swarming motilities, decreased survival ability under oxidative and nitrosative stresses and, consequently, reduced virulence on host tobacco plants. Transmission electron micrographs showed that the structure of the cell membranes of ΔmarR and Δorf5 mutants was severely damaged. Furthermore, not only the ratio of dead cells, but also the amount of flagella, extracellular DNA and protein released into the culture supernatant, was significantly increased in both mutants, indicating that the disruption of marR and orf5 genes might induce structural changes in the membrane and cell lysis. Because both mutants showed partly similar expression profiles, both gene products might be involved in the same regulatory cascades that are required for QS-dependent survival under environmentally stressed conditions.

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  • Expression of Medicago truncatula ecto-apyrase MtAPY1;1 in leaves of Nicotiana benthamiana restricts necrotic lesions induced by a virulent fungus Reviewed

    Kazuhiro Toyoda, Eriko Kawakami, Hideaki Nagai, Taiki Shiobara-Komatsu, Kaori Tanaka, Yoshishige Inagaki, Yuki Ichinose, Tomonori Shiraishi

    JOURNAL OF GENERAL PLANT PATHOLOGY   80 ( 3 )   222 - 229   2014.5

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    Ecto-apyrase(s) participates in cell-wall-associated defense through ATP hydrolysis. Here we analyzed Medicago truncatula genes through cDNA screening and in silico analyses against known databases. This study revealed seven genes, five of which (MtAPY1;1 to MtAPY1;5) are members of a legume-specific family, whereas two genes (MtAPY2;1 and MtAPY2;2) are close to those in other plants. Agrobacterium-based transient expression in Nicotiana benthamiana, combined with a c-myc epitope tag technology, confirmed that the MtAPY1;1 is a secreted protein. Transient expression of MtAPY1;1 in leaves of N. benthamiana restricted disease development by a virulent fungus, suggesting a role in disease resistance.

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  • Ralstonia solanacearum type III secretion system effector Rip36 induces a hypersensitive response in the nonhost wild eggplant Solanum torvum

    Kamrun Nahar, Iyo Matsumoto, Fumiko Taguchi, Yoshishige Inagaki, Mikihiro Yamamoto, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose, Takafumi Mukaihara

    MOLECULAR PLANT PATHOLOGY   15 ( 3 )   297 - 303   2014.4

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    Ralstonia solanacearum is a Gram-negative soil-borne bacterium that causes bacterial wilt disease in more than 200 plant species, including economically important Solanaceae species. In R. solanacearum, the hypersensitive response and pathogenicity (Hrp) type III secretion system is required for both the ability to induce the hypersensitive response (HR) in nonhost plants and pathogenicity in host plants. Recently, 72 effector genes, called rip (Ralstonia protein injected into plant cells), have been identified in R. solanacearum RS1000. RS1002, a spontaneous nalixidic acid-resistant derivative of RS1000, induced strong HR in the nonhost wild eggplant Solanum torvum in an Hrp-dependent manner. An Agrobacterium-mediated transient expression system revealed that Rip36, a putative Zn-dependent protease effector of R. solanacearum, induced HR in S. torvum. A mutation in the putative Zn-binding motif (E149A) completely abolished the ability to induce HR. In agreement with this result, the RS1002-derived Delta rip36 and rip36E149A mutants lost the ability to induce HR in S. torvum. An E149A mutation had no effect on the translocation of Rip36 into plant cells. These results indicate that Rip36 is an avirulent factor that induces HR in S. torvum and that a putative Zn-dependent protease motif is essential for this activity.

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  • Ralstonia solanacearum type III secretion system effector Rip36 induces a hypersensitive response in the nonhost wild eggplant Solanum torvum. International journal

    Kamrun Nahar, Iyo Matsumoto, Fumiko Taguchi, Yoshishige Inagaki, Mikihiro Yamamoto, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose, Takafumi Mukaihara

    Molecular plant pathology   15 ( 3 )   297 - 303   2014.4

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    Ralstonia solanacearum is a Gram-negative soil-borne bacterium that causes bacterial wilt disease in more than 200 plant species, including economically important Solanaceae species. In R. solanacearum, the hypersensitive response and pathogenicity (Hrp) type III secretion system is required for both the ability to induce the hypersensitive response (HR) in nonhost plants and pathogenicity in host plants. Recently, 72 effector genes, called rip (Ralstonia protein injected into plant cells), have been identified in R. solanacearum RS1000. RS1002, a spontaneous nalixidic acid-resistant derivative of RS1000, induced strong HR in the nonhost wild eggplant Solanum torvum in an Hrp-dependent manner. An Agrobacterium-mediated transient expression system revealed that Rip36, a putative Zn-dependent protease effector of R. solanacearum, induced HR in S. torvum. A mutation in the putative Zn-binding motif (E149A) completely abolished the ability to induce HR. In agreement with this result, the RS1002-derived Δrip36 and rip36E149A mutants lost the ability to induce HR in S. torvum. An E149A mutation had no effect on the translocation of Rip36 into plant cells. These results indicate that Rip36 is an avirulent factor that induces HR in S. torvum and that a putative Zn-dependent protease motif is essential for this activity.

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  • Dynamics of signal perception, signaling and regulation of defenses in plant cell walls.

    TOYODA K., TANAKA K., INAGAKI Y., ICHINOSE Y., SHIRAISHI T.

    Japanese Journal of Phytopathology   80 ( 3 )   146 - 151   2014

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    The plant cell wall is well known to act as a physical barrier against invading pathogens. However, our recent studies have suggested that the plant cell wall can perceive pathogen signals and is involved in signaling and regulation of cell wall-based defenses. Here we will focus on a recently discovered role(s) of the plant cell wall in plant-pathogen interactions to unravel the complicated system underlying plant immunity.

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  • Evidence for negative-strand RNA virus infection in fungi Reviewed

    Hideki Kondo, Sotaro Chiba, Kazuhiro Toyoda, Nobuhiro Suzuki

    Virology   435 ( 2 )   201 - 209   2013.1

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    Fungal viruses comprise two groups: a major group of five families with double-stranded RNA genomes and a minor group with positive-sense single-stranded (ss)RNA genomes. Although many fungal viruses have been identified, no negative-stranded (-)ssRNA mycoviruses have been reported. Here we present two lines of evidence suggesting the presence of (-)ssRNA viruses in filamentous fungi based on an exhaustive search using extant (-)ssRNA viruses as queries. This revealed (-)ssRNA virus L protein-like sequences in the genome of a phytopathogenic obligate ascomycete, Erysiphe pisi. A similar search for (-)ssRNA viruses in fungal transcriptome shotgun assembly libraries demonstrated that two independent libraries from Sclerotinia homoeocarpa, another phytopathogenic ascomycete, contained several sequences considered to correspond to the entire mononegavirus L gene and likely originating from an infecting (-)ssRNA virus. These results provide strong evidence for both ancient and extant (-)ssRNA virus infections in fungi. © 2012 Elsevier Inc.

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  • Infection-inhibition activity of avenacin saponins against the fungal pathogens Blumeria graminis f. sp. hordei, Bipolaris oryzae, and Magnaporthe oryzae Reviewed

    Yoshi-Shige Inagaki, Yoshiteru Noutoshi, Keiko Fujita, Atsuko Imaoka, Sakae Arase, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    Journal of General Plant Pathology   79 ( 1 )   69 - 73   2013.1

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    Triterpenoid saponins are sugar-modified triterpene derivatives. Cereals and other grasses are generally deficient in these secondary metabolites with the exception of oat. Oat accumulates antimicrobial triterpenoid saponins in its roots. These oat-root-derived compounds, called avenacins, confer broad-spectrum resistance to soil-borne pathogens. Here, we tested the effect of avenacins on the development of infection structures of fungal pathogens Blumeria graminis f. sp. hordei and Bipolaris oryzae and Magnaporthe oryzae. We show that avenacins are able to inhibit the infection process of these phytopathogens on plant hosts. © 2012 The Phytopathological Society of Japan and Springer Japan.

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  • Plant cell walls as suppliers of potassium and sodium ions for induced resistance in pea (Pisum sativum L.) and cowpea (Vigna unguiculata L.) Reviewed

    Masashi Amano, Kazuhiro Toyoda, Akinori Kiba, Yoshishige Inagaki, Yuki Ichinose, Tomonori Shiraishi

    JOURNAL OF GENERAL PLANT PATHOLOGY   79 ( 1 )   12 - 17   2013.1

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    When an elicitor is applied to plants to induce resistance, one of the first detectable events is the efflux of ions from the treated tissue. Here we are the first to demonstrate that an elicitor from Mycosphaerella pinodes evokes leakage of Na+ and K+ ions from isolated cell walls of pea and cowpea in vitro, as observed for epicotyl tissues. Pharmacological experiments showed that this elicitor-stimulated leakage was sensitive to vanadate and N-(3-methylphenyl)biphenyl-4-sulfonamide (NGXT-191), that inhibit a cell wall-associated ATPase (apyrase). Vanadate or NGXT-191 suppressed elicitor-induced superoxide generation and expression of defense genes in vivo. On the basis of these results, we assume that the leakage of these ions, probably associated with an ATP-dependent process(es) in the cell wall, is likely associated with induced defenses of pea and cowpea.

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  • The Medicago truncatula-Mycoshaerella pinodes interaction: a new pathosystem for dissecting the fungal suppressor-mediated plant disease susceptibility in plants. Reviewed

    Toyoda K, Ikeda S, Morikawa J, Hirose M, Maeda A, Suzuki T, Inagaki Y, Ichinose Y, Shiraishi T

    J Gen Plant Pathol,   79 ( (1) )   1 - 11   2013

  • A volatile substance, beta-caryophyllen, from Talaromyces wortmannii promotes growth and tolerance to disease on several plants.

    Yamagiwa, Y, Toyoda, K, Inagaki, Y, Ichinose, Y, Hyakumachi, M, Shiraishi, T

    Sci. Rep. Fac. Agr. Okayama Univ.   102   7 - 14   2013

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  • Isolation and identification of a plant growth-promoting fungus from an agricultural field in Okayama Prefecture.

    Yamagiwa, Y, Toyoda, K, Inagaki, Y, Ichinose, Y, Shiraishi, T

    Sci. Rep. Fac. Agr. Okayama Univ.   102   1 - 6   2013

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  • Type IV pilin is glycosylated in Pseudomonas syringae pv. tabaci 6605 and required for surface motility and virulence Reviewed International journal

    Linh Chi Nguyen, Fumiko Taguchi, Quang Minh Tran, Kana Naito, Masanobu Yamamoto, Mayumi Ohnishi-Kameyama, Hiroshi Ono, Mitsuru Yoshida, Kazuhiro Chiku, Tadashi Ishii, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    Molecular Plant Pathology   13 ( 7 )   764 - 774   2012.9

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    Type IV pilin (PilA) is a major constituent of pilus and is required for bacterial biofilm formation, surface motility and virulence. It is known that mature PilA is produced by cleavage of the short leader sequence of the pilin precursor, followed by methylation of N-terminal phenylalanine. The molecular mass of the PilA mature protein from the tobacco bacterial pathogen Pseudomonas syringae pv. tabaci 6605 (Pta 6605) has been predicted to be 12 329 Da from its deduced amino acid sequence. Previously, we have detected PilA as an approximately 13-kDa protein by immunoblot analysis with anti-PilA-specific antibody. In addition, we found the putative oligosaccharide-transferase gene tfpO downstream of pilA. These findings suggest that PilA in Pta 6605 is glycosylated. The defective mutant of tfpO (ΔtfpO) shows reductions in pilin molecular mass, surface motility and virulence towards host tobacco plants. Thus, pilin glycan plays important roles in bacterial motility and virulence. The genetic region around pilA was compared among P. syringae pathovars. The tfpO gene exists in some strains of pathovars tabaci, syringae, lachrymans, mori, actinidiae, maculicola and P. savastanoi pv. savastanoi. However, some strains of pathovars tabaci, syringae, glycinea, tomato, aesculi and oryzae do not possess tfpO, and the existence of tfpO is independent of the classification of pathovars/strains in P. syringae. Interestingly, the PilA amino acid sequences in tfpO-possessing strains show higher homology with each other than with tfpO-nonpossessing strains. These results suggest that tfpO and pilA might co-evolve in certain specific bacterial strains.

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  • Genes expressed in tissue-cultured seedlings of mountain laurel (Kalmia latifolia L.) with colonizing Streptomyces padanus AOK30 Reviewed

    Akane Meguro, Kazuhiro Toyoda, Hiroshi Ogiyama, Sachiko Hasegawa, Tomio Nishimura, Hitoshi Kunoh, Tomonori Shiraishi

    JOURNAL OF GENERAL PLANT PATHOLOGY   78 ( 5 )   303 - 310   2012.9

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    An endophytic actinomycete, Streptomyces padanus AOK30, is capable of protecting mountain laurel against infection by Pestalotiopsis sydowiana, a causal agent of Pestalotia disease, when applied to the seedling of the plant. In this study, suppression subtractive hybridization was used to identify genes differentially expressed in seedlings of mountain laurel after application of S. padanus AOK30. Subsequent dot hybridization with independent RNA from S. padanus-colonized and control plants identified nonredundant 180 cDNAs involving 71 and 109 clones, which were up- and downregulated after inoculation with the bacteria, respectively. Comparison of the sequences with databases revealed that a number of transcripts encoding proteins or enzymes that function directly in defense or stress response and regulatory proteins were regulated differentially in the seedlings with colonizing S. padanus AOK30. Semi-quantitative RT-PCR analysis for the selected genes demonstrated that inoculation of mountain laurel seedlings with S. padanus AOK30 increased expression of defense-related genes as well as distinct classes of glutathione S-transferase, although endochitinases were exclusively suppressed. These results clearly indicate that the S. padanus-colonizing seedlings likely initiate or prime plant defense responses toward pathogen infection. Selected genes were also differentially expressed in S. padanus-colonized seedlings, compared to those solely challenged with the fungal pathogen P. sydowiana. This approach will assist in efforts not only to understand the molecular basis of the enhanced tolerance and/or enhanced disease resistance of mountain laurel, but also to define a core set of genes during colonization or association with S. padanus AOK30.

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  • Characterization of each aefR and mexT mutant in Pseudomonas syringae pv. tabaci 6605 Reviewed

    Yuichiro Kawakita, Fumiko Taguchi, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    MOLECULAR GENETICS AND GENOMICS   287 ( 6 )   473 - 484   2012.6

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    To investigate the mechanism of activation of the genes for resistance-nodulation-division (RND) family members MexE, MexF, and OprN for multidrug resistance (MDR), we mutagenized aefR and mexT, the potential regulators of mexEF/oprN transcription in Pseudomonas syringae pv. tabaci 6605 (Pta 6605). AefR is a member of the TetR transcription factors, and is known to be required for production of the quorum-sensing molecules, acyl homoserine lactones (AHL), in P. syringae. Furthermore, we found that AHL-synthesis-defective mutant strains in Pta 6605 showed enhanced expression of mexEF/oprN, and were highly tolerant to antimicrobial compounds such as chloramphenicol. MexT is a LysR-type transcription factor and is known to positively regulate transcription of mexEF/oprN in Pseudomonas aeruginosa. The a dagger aefR mutant reduced the amount of growth in in vitro culture, caused the loss of AHL production, reduced the swarming motility, virulence and expression of psyI (AHL synthase) and psyR (AHL transcriptional regulator), and enhanced mexEF/oprN expression and tolerance to chloramphenicol, whereas the a dagger mexT mutant retained the ability to produce AHL and did not show remarkable changes in in vitro growth, tolerance to antimicrobial compounds or virulence. Furthermore, unlike P. aeruginosa, the expression of mexEF/oprN is independent of MexT. These results indicate that (1) AefR is a regulator for the quorum-sensing system and MDR, and is required for swarming motility and virulence toward the host tobacco plant, and (2) MexT is not involved in the expression of mexEF/oprN in this bacterium.

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  • H2O2 production by copper amine oxidase, a component of the ecto-apyrase (ATPase)-containing protein complex(es) in the pea cell wall, is regulated by an elicitor and a suppressor from Mycosphaerella pinodes. Reviewed

    Toyoda K, Yasunaga E, Niwa M, Ohwatari Y, Nakashima A, Inagaki Y, Ichinose Y, Shiraishi T

    J Gen Plant Pathol,   78 ( (5) )   311 - 315   2012

  • Talaromyces wortmannii FS2 emits beta-caryphyllene, which promotes plant growth and induces resistance Reviewed

    Yasuo Yamagiwa, Yoshishige Inagaki, Yuki Ichinose, Kazuhiro Toyoda, Mitsuro Hyakumachi, Tomonori Shiraishi

    JOURNAL OF GENERAL PLANT PATHOLOGY   77 ( 6 )   336 - 341   2011.11

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    A plant-growth-promoting fungus (PGPF), Talaromyces sp. was isolated from an agricultural field in southwestern Japan. We found that this fungus emitted several terpenoid-like volatiles including beta-caryophyllene. Then we investigated the effect of beta-caryophyllene on promoting the growth and inducing resistance of Brassica campestris L. var. perviridis. The compound significantly enhanced the growth of seedlings and their resistance to Colletotrichum higginsianum. On the basis of these results, we discuss the role of beta-caryophyllene in the activities of PGPF.

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  • Degeneration of hrpZ gene in Pseudomonas syringae pv. tabaci to evade tobacco defence: an arms race between tobacco and its bacterial pathogen Reviewed

    Kazuhiko Tsunemi, Fumiko Taguchi, Mizuri Marutani, Megumi Watanabe-Sugimoto, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    MOLECULAR PLANT PATHOLOGY   12 ( 7 )   709 - 714   2011.9

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    The HrpZ harpin of Pseudomonas syringae is known to induce a hypersensitive response (HR) in some plants. In P. syringae pv. tabaci (Pta), the harpin gene hrpZ has been spontaneously disrupted by an internal deletion in its open reading frame and a frame shift. The loss of the ability of the recombinant harpin polypeptide of Pta to induce HR despite the high sensitivity of tobacco plants to harpin led us to investigate the meaning of the disrupted hrpZ gene in the virulence of Pta 6605. The hrpZ gene from P. syringae pv. pisi was introduced into wild-type (WT) Pta. The hrpZ-complemented Pta secreted harpin into the culture medium, but failed to cause disease symptoms by both infiltration and spray inoculation. Inoculation with the hrpZ-complemented Pta induced defence responses in tobacco plants, whereas the defence responses of tobacco plants were not prominent on inoculation with WT Pta. These results indicate that an ancestor of Pta might have disrupted hrpZ by an internal deletion to evade plant defences and confer the ability to cause disease in tobacco plants.

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  • Two flagellar stators and their roles in motility and virulence in Pseudomonas syringae pv. tabaci 6605 Reviewed

    Eiko Kanda, Takafumi Tatsuta, Tomoko Suzuki, Fumiko Taguchi, Kana Naito, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    MOLECULAR GENETICS AND GENOMICS   285 ( 2 )   163 - 174   2011.2

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    The motor proteins around the flagellar basal body consist of two cytoplasmic membrane proteins, MotA and MotB, and function as a complex that acts as the stator to generate the torque that drives rotation. Genome analysis of several Pseudomonas syringae pathovars revealed that there are two sets of genes encoding motor proteins: motAB and motCD. Deduced amino acid sequences for MotA/B and MotC/D showed homologies to the H(+)-driven stator from Escherichia coli and Na(+)-driven stator from Vibrio alginolyticus, respectively. However, the swimming motility of P. syringae pv. tabaci (Pta) 6605 was inhibited by the protonophore carbonyl cyanide m-chlorophenylhydrazone but not by the sodium stator-specific inhibitor phenamil. To identify a gene encoding the stator protein required for motility, a dagger motAB, a dagger motCD, and a dagger motABCD mutants were generated. The a dagger motCD mutant had remarkably reduced and the a dagger motABCD mutant completely abolished swimming motilities, whereas the a dagger motAB mutant retained some degree of these abilities. The a dagger motCD and a dagger motABCD mutants did not produce N-acyl-homoserine lactones (AHLs), quorum-sensing molecules in this pathogen, and remarkably reduced the ability to cause disease in host tobacco leaves, as we previously observed in the a dagger fliC mutant strain. These results strongly indicate that both stator pairs in Pta 6605 are proton-dependent and that MotCD is important for not only flagellar motility but also for production of AHLs and the ability to cause disease in host plants.

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  • Siderophore pyoverdine of Pseudomonas syringae pv. tabaci 6605 is an intrinsic virulence factor in host tobacco infection. Reviewed

    Taguchi F, Suzuki T, Inagaki Y, Toyoda K, Shiraishi T, Ichinose Y

    J. Bacteriol.   192 ( 1 )   117 - 126   2010

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    To investigate the role of iron uptake mediated by the siderophore pyoverdine in the virulence of the plant pathogen Pseudomonas syringae pv. tabaci 6605, three predicted pyoverdine synthesis-related genes, pvdJ, pvdL, and fpvA, were mutated. The pvdJ, pvdL, and fpvA genes encode the pyoverdine side chain peptide synthetase III L-Thr-L-Ser component, the pyoverdine chromophore synthetase, and the TonB-dependent ferripyoverdine receptor, respectively. The Delta pvdJ and Delta pvdL mutants were unable to produce pyoverdine in mineral salts glucose medium, which was used for the iron-depleted condition. Furthermore, the Delta pvdJ and Delta pvdL mutants showed lower abilities to produce tabtoxin, extracellular polysaccharide, and acyl homoserine lactones (AHLs), which are quorum-sensing molecules, and consequently had reduced virulence on host tobacco plants. In contrast, all of the mutants had accelerated swarming ability and increased biosurfactant production, suggesting that swarming motility and biosurfactant production might be negatively controlled by pyoverdine. Scanning electron micrographs of the surfaces of tobacco leaves inoculated with the mutant strains revealed only small amounts of extracellular polymeric matrix around these mutants, indicating disruption of the mature biofilm. Tolerance to antibiotics was drastically increased for the Delta pvdL mutant, as for the Delta psyI mutant, which is defective in AHL production. These results demonstrated that pyoverdine synthesis and the quorum-sensing system of Pseudomonas syringae pv. tabaci 6605 are indispensable for virulence in host tobacco infection and that AHL may negatively regulate tolerance to antibiotics.

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  • Glycosylation of flagellin from Pseudomonas syringae pv. tabaci 6605 contributes to evasion of host tobacco plant surveillance system Reviewed

    Fumiko Taguchi, Tomoko Suzuki, Kasumi Takeuchi, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY   74 ( 1 )   11 - 17   2009.1

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    Pseudomonas syringae pv. tabaci (Pta) possesses a genetic region composed of two open reading frames (ORFs), fgt1 and fgt2, that are involved in glycosylation of flagellin. The deletion mutant Delta fgt1 produced non-glycosylated flagellin, and exhibited reduced ability to cause disease in the host tobacco plant. Flagellin is known to induce plant defense responses, and the recognition of flagellin by Arabidopsis thaliana is mediated by a conserved N-terminal region, flg22, in flagellin and a leucine-rich repeat domain in the FLS2 receptor. Because flg22 localizes inside the flagellum, polymerized flagellum needs to be dissociated to be recognized. Therefore, the effect of glycosylation on flagella stability was investigated. The polymerized flagella from glycosylated flagellins were more resistant to heat treatment than those from non-glycosylated flagellins, suggesting that the glycosylation of flagellin contributes to the structural stability of flagella and prevents exposure of the flg22 region. Polymerized flagella from Pta Delta fgt1 flagellin and depolymerized and glycosylated flagellin from Pta wild type induced cell death and callose deposition, and inhibited seedling growth in tobacco more effectively, whereas polymerized flagella from Pta wild-type flagellin caused a low level of these responses. These results suggest Pta might have evolved the flagellin glycosylation system to evade detection and defense response of a host by increasing flagella stability and suppressing their dissociation. (C) 2009 Elsevier Ltd. All rights reserved.

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  • Amino Acid Sequence of Bacterial Microbe-Associated Molecular Pattern flg22 Is Required for Virulence Reviewed

    Kana Naito, Fumiko Taguchi, Tomoko Suzuki, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    MOLECULAR PLANT-MICROBE INTERACTIONS   21 ( 9 )   1165 - 1174   2008.9

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    Flagellin proteins derived from Pseudomonas syringae pv. tabaci 6605 and flg22(Pa) (QRLSTGSRINSAKDDAAGLQIA), one of the microbe-associated molecular patterns (MAMP) in bacterial flagellin, induce cell death and growth inhibition in Arabidopsis thaliana. To examine the importance of aspartic acid (D) at position 43 from the N-terminus of a flagellin in its elicitor activity, D43 was replaced with valine (V) and alanine (A) in P. syringae pv. tabaci flagellin and flg22(Pta). The abilities of flagellins from P syringae pv. tabaci D43V and D43A to induce cell death and growth inhibition were reduced, whereas the abilities of flg22(Pta)D43V and flg22(Pta)D43A were abolished. These results indicate that D43 is important for elicitor activity in P. syringae pv. tabaci. When tobacco plants were inoculated with each bacterium by the spray method, both P. syringae pv. tabaci D43V and D43A mutants had remarkably reduced ability to cause disease symptoms. Both mutants had reduced or no swimming and swarming motilities and adhesion ability. In P. syringae pv. tabaci D43V, little flagellin protein was detected and few flagella were observed by electron microscopy. These results indicate that mutant flagella are unstable and that flagellar motility is impaired. Thus, the amino acid residue required for MAMP activity is important for the intrinsic flagellar function.

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  • Gac two-component system in Pseudomonas syringae pv. tabaci is required for virulence but not for hypersensitive reaction Reviewed

    Mizuri Marutani, Fumiko Taguchi, Yujiro Ogawa, Md. Mijan Hossain, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    MOLECULAR GENETICS AND GENOMICS   279 ( 4 )   313 - 322   2008.4

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    Pseudomonas syringae pv. tabaci 6605 causes wildfire disease on host tobacco plants. To investigate the regulatory mechanism of the expression of virulence, Gac two-Component system-defective mutants, Delta gacA and Delta gacS, and a double mutant, Delta gacA Delta gacS, were generated. These mutants produced smaller amounts of N-acyl homoserine lactones required for quorum sensing, had lost swarming motility, and had reduced expression of virulence-related hrp genes and the algT gene required for exopolysaccharide production. The ability of the mutants to cause disease symptoms in their host tobacco plant was remarkably reduced, while they retained the ability to induce hypersensitive reaction (HR) in the nonhost plants. These results indicated that the Gac two-component system of P. syringae pv. tabaci 6605 is indispensable for virulence on the host plant, but not for HR induction in the nonhost plants.

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  • The model plant Medicago truncatula exhibits biparental plastid inheritance Reviewed

    Ryo Matsushima, Yingchun Hu, Kazuhiro Toyoda, Sodmergen, Wataru Sakamoto

    PLANT AND CELL PHYSIOLOGY   49 ( 1 )   81 - 91   2008.1

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    The plastid, which originated from the endosymbiosis of a cyanobacterium, contains its own plastid DNA (ptDNA) that exhibits a unique mode of inheritance. Approximately 80 of angiosperms show maternal inheritance, whereas the remainder exhibit biparental inheritance of ptDNA. Here we studied ptDNA inheritance in the model legume, Medicago truncatula. Cytological analysis of mature pollen with DNA-specific fluorescent dyes suggested that M. truncatula is one of the few model plants potentially showing biparental inheritance of ptDNA. We further examined pollen by electron microscopy and revealed that the generative cell (a mother of sperm cells) indeed has many DNA-containing plastids. To confirm biparental inheritance genetically, we crossed two ecotypes (Jemalong A17 and A20), and the transmission mode of ptDNA was investigated by a PCR-assisted polymorphism. Consistent with the cytological observations, the majority of F-1 plants possessed ptDNAs from both parents. Interestingly, cotyledons of F-1 plants tended to retain a biparental ptDNA population, while later emergent leaves tended to be uniparental with either one of the parental plastid genotypes. Biparental transmission was obvious in the F-2 population, in which all plants showed homoplasmy with either a paternal or a maternal plastid genotype. Collectively, these data demonstrated that M. truncatula is biparental for ptDNA transmission and thus can be an excellent model to study plastid genetics in angiosperms.

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  • Suppression of Cdc27B expression induces plant defence responses Reviewed

    Chikako Kudo, Tomoko Suzuki, Sumie Fukuoka, Shuta Asai, Hiroko Suenaga, Michiko Sasabe, Yoshitaka Takano, Tetsuro Okuno, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose, Yoshi-Shige Inagaki

    MOLECULAR PLANT PATHOLOGY   8 ( 4 )   365 - 373   2007.7

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    Non-host resistance is the most general form of disease resistance in plants because it is effective against most phytopathogens. The importance of hypersensitive responses (HRs) in non-host resistance of Nicotiana species to the oomycete Phytophthora is clear. INF1 elicitin, an elicitor obtained from the late-blight pathogen Phytophthora infestans, is sufficient to induce a typical HR in Nicotiana species. The molecular mechanisms that underlie the non-host resistance component of plant defence responses have been investigated using differential-display polymerase chain reaction (PCR) in a model HR system between INF1 elicitin and tobacco BY-2 cells. Differential-display PCR has revealed that Cdc27B is down-regulated in tobacco BY- 2 cells after treatment with INF1 elicitin. Cdc27B is one of 13 essential components of the anaphase- promoting complex or cyclosome ( APC/ C)-type E3 ubiquitin ligase complex in yeast. This APC/C-type E3 ubiquitin ligase complex regulates G2-to-M phase transition of the cell cycle by proteolytic degradation. In this study, we investigated the roles of this gene, NbCdc27B, in plant defence responses using virus-induced gene silencing. Suppression of NbCdc27B in Nicotiana benthamiana plants induced defence responses and a gain of resistance to Colletotrichum lagenarium fungus. Elicitin-induced hypersensitive cell death (HCD) was inhibited mildly in plants silenced with tobacco rattle virus:: Cdc27B. Cdc27B could manage the signalling pathways of plant defence responses as a negative regulator without HCD.

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  • Elicitin-responsive lectin-like receptor kinase genes in BY-2 cells Reviewed

    Michiko Sasabe, Kana Naito, Hiroko Suenaga, Takako Ikeda, Kazuhiro Toyoda, Yoshishige Inagaki, Tomonori Shiraishi, Yuki Ichinose

    DNA Sequence - Journal of DNA Sequencing and Mapping   18 ( 2 )   152 - 159   2007

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    The inhibition of elicitor-induced plant defense responses by the protein kinase inhibitors K252a and staurosporine indicates that defense responses require protein phosphorylation. We isolated a cDNA clone encoding Nicotiana tabacum lectin-like receptor protein kinase 1 (NtlecRK1), an elicitor-responsive gene
    in tobacco bright yellow (BY-2) cells by a differential display method. NtlecRK forms a gene family with at least three members in tobacco. All three NtlecRK genes potentially encode the N-terminal legume lectin domain, transmembrane domain and C-terminal Ser/Thr-type protein kinase domain. Green fluorescent protein (GFP) fusion showed that the NtlecRK1 protein was located on the plasma membrane. In addition, NtlecRK1 and 3 were responsive to INF1 elicitin and the bacterial elicitor harpin. These results indicate that NtlecRKs are membrane-located protein kinases that are induced during defense responses in BY-2 cells. © 2007 Informa UK Ltd.

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  • Localization and responsiveness of a cowpea apyrase VsNTPase1 to phytopathogenic microorganisms. Reviewed

    Hirotaka Takahashi, Kazuhiro Toyoda, Yuzo Hirakawa, Kunihiko Morishita, Toshiaki Kato, Yoshishige Inagaki, Yuki Ichinose, Tomonori Shiraishi

    Journal of General Plant Pathology   72 ( 3 )   143 - 151   2006.11

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  • Identification of genes expressed during spore germination of Mycosphaerella pinodes Reviewed

    Hiroyuki Takahara, Kazuhiro Toyoda, Gento Tsuji, Yasuyuki Kubo, Yoshishige Inagaki, Yuki Ichinose, Tomonori Shiraishi

    Journal of General Plant Pathology   71 ( 3 )   190 - 195   2005.6

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    Mycosphaerella blight, caused by Mycosphaerella pinodes, is one of the major diseases of cultivated pea (Pisum sativum L.). To isolate the genes that are up- and down-regulated during spore germination, suppression subtraction hybridization (SSH) was performed between ungerminated and germinated spores. The 232 and 128 clones from forward and reverse libraries, respectively, were collected, sequenced, and analyzed with a BLASTX homology search. About 95% of the 32 selected clones were expressed during spore germination on a paper sheet and during infection of pea leaves. We discuss the applicability of the SSH libraries for analyzing M. pinodes genes involved in the early stage of infection. © The Phytopathological Society of Japan and Springer-Verlag 2005.

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  • Discrimination between tobamoviruses and their pathotypes for L-gene-mediated resistance in green pepper (Capsicum annuum L.) by reverse transcription-polymerase chain reaction Reviewed

    Shigeharu Takeuchi, Hiroyuki Hamada, Kazuhiro Toyoda, Kazumi Suzuki, Akinori Kiba, Yasufumi Hikichi, Tetsuro Okuno

    Journal of General Plant Pathology   71 ( 1 )   60 - 67   2005.2

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    A new method to discriminate between tobamoviruses and their pathotypes that infect green pepper (Capsicum annuum L.) was developed using reverse transcription-polymerase chain reaction (RT-PCR). The P0 pathotype (Tobacco mosaic virus, Tomato mosaic virus, and Tobacco mild green mosaic virus) and the P1 pathotype (Paprika mild mottle virus) were distinguished by RT-PCR using primers specific to each pathotype. However, the P1,2 and P1,2,3 pathotypes of Pepper mild mottle virus (PMMoV) could not be distinguished from each other using this procedure. The P1,2 and P1,2,3 pathotypes were differentiated by RT-nested PCR, in which a DNA fragment was first produced by RT-PCR using primers containing conserved sequences of PMMoV. The product was then used as the DNA template in a second PCR using primers specific to each pathotype. An immunocapture (IC) RT-PCR method was developed based on results from this study, which facilitated detection of tobamoviruses in pepper plants, seeds, and field soils and allowed the identification of their pathotypes. © The Phytopathological Society of Japan and Springer-Verlag 2005.

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  • Regulation of elicitin-induced ethylene production in suspension cultured tobacco BY-2 cells. Reviewed

    Schenke D, Naito K, Toyoda K, Inagaki Y, Shiraishi T, Ichinose Y

    J. Gen. Plant Pathol.   71   273 - 279   2005

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  • Agrobacterium tumefaciens-mediated transformation as a tool for random mutagenesis of Colletotrichum trifolii Reviewed

    Hiroyuki Takahara, Gento Tsuji, Gento Tsuji, Yasuyuki Kubo, Mikihiro Yamamoto, Kazuhiro Toyoda, Yoshishige Inagaki, Yuki Ichinose, Tomonori Shiraishi

    Journal of General Plant Pathology   70 ( 2 )   93 - 96   2004.4

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    We transformed Colletotrichum trifolii, the causal agent of alfalfa anthracnose, using Agrobacterium tumefaciens as a new tool for random insertional mutagenesis. Fungal spores of C. trifolii were transformed with T-DNA including the hygromycin phosphotransferase gene (hph). Southern analysis showed that every randomly selected transformant had a unique hybridization pattern of T-DNA, suggesting that the T-DNA was randomly integrated into the fungal genome. More significantly, about 75% of transformants had a single copy of the T-DNA. The results demonstrate that insertional mutagenesis via A. tumefaciens is a useful tool for studying the function of C. trifolii genes.

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  • Structure and expression of 12-oxophytodienoate reductase (subgroup I) genes in pea, and characterization of the oxidoreductase activities of their recombinant products Reviewed

    H Matsui, G Nakamura, Y Ishiga, H Toshima, Y Inagaki, K Toyoda, T Shiraishi, Y Ichinose

    MOLECULAR GENETICS AND GENOMICS   271 ( 1 )   1 - 10   2004.2

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    Recently, we observed that expression of a pea gene (S64) encoding an oxophytodienoic acid reductase (OPR) was induced by a suppressor of pea defense responses, secreted by the pea pathogen Mycosphaerella pinodes. Because it is known that OPRs are usually encoded by families of homologous genes, we screened for genomic and cDNA clones encoding members of this putative OPR family in pea. We isolated five members of the OPR gene family from a pea genomic DNA library, and amplified six cDNA clones, including S64, by RT-PCR (reverse transcriptase-PCR). Sequencing analysis revealed that S64 corresponds to PsOPR2, and the amino acid sequences of the predicted products of the six OPR-like genes shared more than 80% identity with each other. Based on their sequence similarity, all these OPR-like genes code for OPRs of subgroup I, i.e., enzymes which are not required for jasmonic acid biosynthesis. However, the genes varied in their exon/intron organization and in their promoter sequences. To investigate the expression of each individual OPR-like gene, RT-PCR was performed using gene-specific primers. The results indicated that the OPR-like gene most strongly induced by the inoculation of pea plants with a compatible pathogen and by treatment with the suppressor from M. pinodes was PsOPR2. Furthermore, the ability of the six recombinant OPR-like proteins to reduce a model substrate, 2-cyclohexen-1-one (2-CyHE), was investigated. The results indicated that PsOPR1, 4 and 6 display robust activity, and PsOPR2 has a most remarkable ability to reduce 2-CyHE, whereas PsOPR3 has little and PsOPR5 does not reduce this compound. Thus, the six OPR-like proteins can be classified into four types. Interestingly, the gene structures, expression profiles, and enzymatic activities used to classify each member of the pea OPR-like gene family are clearly correlated, indicating that each member of this OPR-like family has a distinct function.

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  • Genomic structure of the NtPDR1 gene, harboring the two miniature inverted-repeat transposable elements, NtToya1 and NtStowaway101

    SCHENKE Dirk, SASABE Michiko, TOYODA Kazuhiro, INAGAKI Yoshi-shige, SHIRAISHI Tomonori, ICHINOSE Yuki

    The Japanese Journal of Genetics   78 ( 6 )   409 - 418   2003.12

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    Here we report the genomic structure including the promoter sequence and coding region of NtPDR1 (Nicotiana tabacum Pleiotropic Drug Resistance 1), which is an elicitor-responsive gene encoding an ATP binding cassette (ABC) transporter that might be involved in the defense response in tobacco, as we reported recently. The NtPDR1 gene consists of 20 exons and 19 introns. Among the introns, the first and fifth are much larger than the others and harbor typical miniature inverted-repeat transposable elements (MITEs). One of the MITE elements in the first intron, termed NtToya1, belongs to the Toya family that was recently described in rice, while the other element in the fifth intron, termed NtStowaway101, shows high homology with the Stowaway elements of the IS630-Tc1-mariner family. Many of the genes we found to harbor Toya and Stowaway elements in Nicotiana species by BLAST search are also involved in stress responses or plant-pathogen interactions. The existence of putative cis-elements (a GCC box, three W boxes, and several JA-responsive elements) in the promoter region supports our previous finding that this gene is strongly inducible by elicitation and methyljasmonate, and that this ABC transporter might be essential for plant defense responses. Furthermore, Southern blot analysis and PCR amplification of the introns harboring the MITE-like elements from genomic DNA of three Nicotiana species suggests that NtPDR1 originated from N. sylvestris.<br>

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  • Flagellin glycosylation island in Pseudomonas syringae pv. glycinea and its role in host specificity. Reviewed International journal

    Kasumi Takeuchi, Fumiko Taguchi, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

    Journal of bacteriology   185 ( 22 )   6658 - 65   2003.11

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    The deduced amino acid sequences of the flagellins of Pseudomonas syringae pv. tabaci and P. syringae pv. glycinea are identical; however, their abilities to induce a hypersensitive reaction are clearly different. The reason for the difference seems to depend on the posttranslational modification of the flagellins. To investigate the role of this posttranslational modification in the interactions between plants and bacterial pathogens, we isolated genes that are potentially involved in the posttranslational modification of flagellin in P. syringae pv. glycinea (glycosylation island); then defective mutants with mutations in these genes were generated. There are three open reading frames in the glycosylation island, designated orf1, orf2, and orf3. orf1 and orf2 encode putative glycosyltransferases, and mutants with defects in these open reading frames, deltaorf1 and deltaorf2, secreted nonglycosylated and slightly glycosylated flagellins, respectively. Inoculation tests performed with these mutants and original nonhost tobacco leaves revealed that deltaorf1 and deltaorf2 could grow on tobacco leaves and caused symptom-like changes. In contrast, these mutants failed to cause symptoms on original host soybean leaves. These data indicate that putative glycosyltransferases encoded in the flagellin glycosylation island are strongly involved in recognition by plants and could be the specific determinants of compatibility between phytopathogenic bacteria and plant species.

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  • The Delta fliD mutant of Pseudomonas syringae pv. tabaci, which secretes flagellin monomers, induces a strong hypersensitive reaction (HR) in non-host tomato cells Reviewed

    R Shimizu, F Taguchi, M Marutani, T Mukaihara, Y Inagaki, K Toyoda, T Shiraishi, Y Ichinose

    MOLECULAR GENETICS AND GENOMICS   269 ( 1 )   21 - 30   2003.4

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    To investigate the role of flagella and monomer flagellin in the interaction between Pseudomonas syringae pv. tabaci and plants, non-polar fliC and fliD mutants were produced. The ORFs for fliC and fliD are deleted in the DeltafliC and DeltafliD mutants, respectively. Both mutants lost all flagella and were non-motile. The DeltafliC mutant did not produce flagellin, whereas the DeltafliD mutant, which lacks the HAP2 protein, secreted large amounts of monomer flagellin into the culture medium. Inoculation of non-host tomato leaves with wild-type P. syringae pv. tabaci or the DeltafliD mutant induced a hypersensitive reaction (HR), whereas the Deltaflid mutant propagated and caused characteristic symptom-like changes. In tomato cells in suspension culture, wild-type P. syringae pv. tabaci induced slight, visible HR-like changes. The DeltafliC mutant did not induce HR, but the DeltafliD mutant induced a remarkably strong HR. Expression of the hsr203J gene was rapidly and strongly induced by inoculation with the DeltafliD mutant, compared to inoculation with wild-type P. syringae pv. tabaci. Furthermore, introduction of the fliC gene into the DeltafliC mutant restored motility and HR-inducing ability in tomato. These results, together with our previous study, suggest that the flagellin monomer of pv. tabaci acts as a strong elicitor to induce HR-associated cell death in non-host tomato cells.

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  • Molecular Cloning of cDNA for a Novel Pea Dof Protein, PsDof1, and Its DNA-Binding Activity to the Promoter of PsDof1 Gene

    SEKI Hikaru, NAKAMURA Naoki, MARUTANI Mizuri, OKABE Takeharu, SANEMATSU Shiroh, INAGAKI Yoshishige, TOYODA Kazuhiro, SHIRAISHI Tomonori, YAMADA Tetsuji, ICHINOSE Yuki

    Plant tissue culture letters   19 ( 4 )   251 - 260   2002.12

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    A cDNA clone, designated PsDof1, encoding a novel member of the Dof DNA-binding protein family was isolated from pea. Random-binding-site selection and gel retardation experiments showed that the GST-PsDof1 recombinant protein bound to specific DNA sequences with an AAAG core. A metal-chelating agent, 1, 10-phenanthroline, abolished binding of GST-PsDof1 to DNA, but the addition of Zn2+ restored it, indicating that zinc ions are required for its DNA-binding activity. Interestingly, there are possible sites for binding PsDof1 in its promoter sequence. Indeed, GST-PsDof1 binds to the AAAG-containing promoter sequence of the PsDof1 gene with higher affinity. These results suggest that PsDof1 is a novel transcription factor that is involved in its own transcriptional regulation.

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  • cDNA cloning and characterization of tobacco ABC transporter: NtPDR1 is a novel elicitor-responsive gene Reviewed

    M Sasabe, K Toyoda, T Shiraishi, Y Inagaki, Y Ichinose

    FEBS LETTERS   518 ( 1-3 )   164 - 168   2002.5

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    We isolated an INF1 elicitin-inducible cDNA encoding a pleiotropic drug resistance (PDR)-type ATP-binding cassette (A-BC) transporter homolog (NtPDR1) in suspension-cultured tobacco Bright Yellow-2 (BY-2) cells by application of differential display PCR. The NtPDR1 (Nicotiana tabacum PDR protein 1) gene also encodes a 162 kDa protein that includes two putative hydrophilic domains containing the ABC signature motif and two putative hydrophobic domains. Expression of the NtPDR1 gene was rapidly and strongly activated by treatment of BY-2 cells with INF1 elicitin. Further, treatment of BY-2 cells with flagellin, a bacterial proteinaceous hypersensitive reaction elicitor, or yeast extract, a general elicitor, also induced NtPDR1 gene expression. These results indicate that NtPDR1 may be involved in the general defense response in tobacco. This is the first report that microbial elicitors induce the expression of a plant ABC transporter gene. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

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  • Contrary operations of Box-I element of pea phenylalanine ammonia-lyase gene 1 promoter for organ-specific expression Reviewed

    Y Imura, H Seki, K Toyoda, Y Ichinose, T Shiraishi, T Yamada

    PLANT PHYSIOLOGY AND BIOCHEMISTRY   39 ( 5 )   355 - 362   2001.5

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    Expression of genes (PSPAL) encoding phenylalanine ammonia-lyase from pea (Pisum sativum L.) is regulated in response to various environmental stimuli and during plant development. We examined the cis-regulatory elements in PSPAL1 promoter for organ-specific expression by determining the sequences specifically associated with nuclear proteins on its promoter in each pea organ. In vivo dimethyl sulfate (DMS) footprinting analysis showed putative protein bindings on AC-rich sequences including Box-I in particular in roots and stems in which PSPAL mRNA were highly accumulated. The potential role of the AC-rich element was investigated by fusing PSPAL1 promoter with Box-I deleted to the reporter gene P-glucuronidase (GUS) and transforming the construct into tobacco plants (Nicotiana tabacum). GUS activity controlled under this Box-I deletion promoter was significantly reduced in roots and leaves, whereas drastically increased in stems as compared with the activity of wild-type PSPALI promoter. Histochemical GUS staining indicated the activity was elevated in vascular tissues of stem by deleting Box-I. These results suggest that Box-I in PSPALI contributes to a positive regulation in root and leaf, but might also function as a negative regulator in stem, especially in xylem tissue. (C) 2001 Editions scientifiques et medicales Elsevier SAS.

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  • Molecular cloning and functional analysis of pea cDNA E86 encoding homologous protein to hypersensitivity-related hsr203J Reviewed

    Y Ichinose, Y Hisayasu, S Sanematsu, Y Ishiga, H Seki, K Toyoda, T Shiraishi, T Yamada

    PLANT SCIENCE   160 ( 5 )   997 - 1006   2001.4

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    Clone E86 was isolated as cDNA for elicitor-inducible gene From pea epicotyls by differential screening. The deduced amino acid sequence of E86 showed high homology to hypersensitivity-related protein hsr203J in tobacco and also showed significant homologies to the Ser-active hydrolases, such as mammalian hormone-sensitive lipases, bacterial lipases and esterases. E86 polypeptide possesses consensus amino acid sequence motifs (His-Gly) and (Gly-X-Ser-X-Gly) conserved in lipases and esterases and showed esterase degradation of p-nitrophenyl butyrate. Northern blot analysis revealed that the E86-transcript is abundant in roots and stems and was induced by fungal elicitor in pea epicotyls. However, elicitor-induced accumulation of E86 mRNA was significantly inhibited by the fungal suppressor. Furthermore the expression of the genes encoding E86 and phenylalanine ammonia-lyase was induced within 1 h after the inoculation of a nonpathogen. but it was delayed for 5 h by the inoculation of a compatible pathogen. These results suggest that the elicitor-induced Ser-active hydrolase derived from E86 gene might be related to the plant defense responses. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

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  • Suppressors of defense - Supprescins and plant receptor molecules Reviewed

    T Shiraishi, K Toyoda, T Yamada, Y Ichinose, A Kiba, M Sugimoto

    DELIVERY AND PERCEPTION OF PATHOGEN SIGNALS IN PLANTS   112 - 121   2001

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  • Regulation of nuclear gene expression in relation to signal molecules Reviewed

    T Yamada, Y Ichinose, T Shiraishi, K Toyoda, Y Imura, H Seki, P Sriprasertsak, A Funado

    DELIVERY AND PERCEPTION OF PATHOGEN SIGNALS IN PLANTS   164 - 173   2001

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  • Independent pathways leading to apoptotic cell death, oxidative burst and defense gene expression in response to elicitin in tobacco cell suspension culture Reviewed

    M Sasabe, K Takeuchi, S Kamoun, Y Ichinose, F Govers, K Toyoda, T Shiraishi, T Yamada

    EUROPEAN JOURNAL OF BIOCHEMISTRY   267 ( 16 )   5005 - 5013   2000.8

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    We characterized pharmacologically the hypersensitive cell death of tobacco BY-2 cells that followed treatments with Escherichia coli preparations of INF1, the major secreted elicitin of the late blight pathogen Phytophthora infestans. INF1 elicitin treatments resulted in fragmentation and 180 bp laddering of tobacco DNA as early as 3 h post-treatment. INF1 elicitin also induced rapid accumulation of H2O2 typical of oxidative burst, and the expression of defense genes such as phenylalanine ammonia-lyase (PAL) gene at 1 h and 3 h after elicitin treatment, respectively. To investigate the involvement of the oxidative burst and/or the expression of defense genes in the signal transduction pathways leading to hypersensitive cell death, we analyzed the effect of several chemical inhibitors of signal transduction pathways on the various responses. The results indicated that (a) the cell death required serine proteases, Ca2+ and protein kinases, (b) the oxidative burst was involved in Ca2+ and protein kinase mediated pathways, but elicitin-induced AOS was neither necessary nor sufficient for cell death and PAL gene expression, and (c) the signaling pathway of PAL gene expression required protein kinases. These results suggest that the three signal transduction pathways leading to cell death, oxidative burst and expression of defense genes branch in the early stages that follow elicitin recognition by tobacco cells.

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  • Regulation of Signal Transduction and Expression of Defense Responses in Pea by Elicitor and Suppressor from Mycosphaerella pinodes

    TOYODA Kazuhiro

    Annals of the Phytopathological Society of Japan   65 ( 3 )   247 - 247   1999.6

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  • Plant cell wall with the suppressor may play a crucial role in determining specificity Reviewed

    T Shiraishi, A Kiba, A Inata, T Sugiura, K Toyoda, Y Ichinose, T Yamada

    MOLECULAR GENETICS OF HOST-SPECIFIC TOXINS IN PLANT DISEASES   13   343 - 353   1998

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    The mechanism of plant host-parasite specificity is one of the most intriguing issues of contemporary plant biology. We recently found that a fungal elicitor stimulated cell wall functions such as ATP-hydrolyzing and superoxide generating activities. Furthermore, the suppressor from Mycosphaerella pinodes inhibited these activities in vitro in a strictly species-specific manner. The cell wall-bound ATPase, which is different from that of the plasma membrane in several properties, was associated with cell wall-bound peroxidase(s). In response to the elicitor, the isolated cell walls are able to produce an infection-inhibitor that may be dependent upon the superoxide generating system. On the other baud, the suppressor inhibited such production in pea cell walls and induced it in nonhost's cell walls. Thus, the effects of both fungal signals on isolated cell walls coincide with those on plant tissues. Ln this treatise, we discuss the importance of the cell wall, which is plant-specific acid the most exterior organelle, in determining host-parasite specificity.

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  • Successive Observation of Growth and Movement of Genetically lux-marked Pseudomonas cichorii and the Response of Host Tissues in the Same Lettuce Leaf.

    Yasufumi HIKICHI, Kazumi SUZUKI, Kazuhiro TOYODA, Mamoru HORIKOSHI, Takashi HIROOKA, Tetsuro OKUNO

    Japanese Journal of Phytopathology   64 ( 6 )   519 - 525   1998

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  • Association between ion fluxes and defense responses in pea and cowpea tissues Reviewed

    M Amano, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

    PLANT AND CELL PHYSIOLOGY   38 ( 6 )   698 - 706   1997.6

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    The glycopeptide elicitor from a pea pathogen, Mycosphaerella pinodes, induced rapid alkalinization and increases in levels of Na+ and K+ ions in the extracellular solution upon contact with pea and cowpea tissues, The presence of monensin, nigericin, lidocaine, quinidine or phenytoin together with the elicitor markedly inhibited these changes, whereas the presence of valinomycin, gramicidin D, tetraethylammonium, CsCl and aminopyridine did not. The production of phytoalexins in pea and cowpea tissues was also strongly inhibited by the simultaneous presence of the former reagents but not of the latter reagents. Inhibitory effects on the production of phytoalexins were diminished when monensin, nigericin or a Na+-channel blocker was applied 3 h after the start of treatment with elicitor, Furthermore, orthovanadate and neomycin, which suppress defense responses in both tissues, also inhibited the above mentioned changes. By contrast, the species-specific suppressor from M. pinodes inhibited the elicitor-induced release of Na+ and K+ ions from pea tissues, but, conversely, by itself it elicited either the defense response or the release of Na+ and K+ ions from cowpea tissues. The results indicate that these ion-related changes, in particular the efflux of Na+ and K+ ions, might be closely associated with the signal transduction system for defense responses at the tissue level.

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  • Specific response of partially purified cell wall-bound ATPases to fungal suppressor Reviewed

    A Kiba, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

    PLANT AND CELL PHYSIOLOGY   37 ( 2 )   207 - 214   1996.3

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    It was found that NTPases were bound to cell walls of pea and cowpea. The suppressor in pycnospore germination fluid of a pea pathogen, Mycosphaerella pinodes, inhibited the ATPase activity in the fraction, which was solubilized from pea cell wall with 0.5% Triton X-100, in a dose-dependent manner, but rather enhanced that from cowpea cell wall even at the concentration of 1 mu g ml(-1). Inhibition by the suppressor of pea cell wall-bound ATPase was a mixed type of competitive and noncompetitive. Triton X-100 PAGE and active staining of ATPase indicated that both Triton X-100 solubilized fractions contained plural molecules that hydrolyze ATP. The M(r)s of cell wall-bound ATPases seem to be considerably different from those of plasma membranes, and the number of cell wall-bound ATPase molecules were different between pea and cowpea. The electroeluted fractions corresponding to the bands of active-stained ATPases were also able to hydrolyze NTP and PPi. The respective electroeluted ATPases also showed the species-specific response to the suppressor. These results may confirm our previous concept that putative receptors for the suppressor might tightly bind to cell wall-bound ATPase or that the ATPase might be the receptor itself.

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  • Fungal signals regulate ATPase and polyphosphoinositide metabolism in pea plants Reviewed

    T Shiraishi, T Yamada, Y Ichinose, K Toyoda, A Kiba, T Kato

    MOLECULAR ASPECTS OF PATHOGENICITY AND RESISTANCE: REQUIREMENT FOR SIGNAL TRANSDUCTION   197 - 208   1996

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  • RAPID CHANGES IN POLYPHOSPHOINOSITIDE METABOLISM IN PEA IN RESPONSE TO FUNGAL SIGNALS Reviewed

    K TOYODA, T SHIRAISHI, T YAMADA, Y ICHINOSE, H OKU

    PLANT AND CELL PHYSIOLOGY   34 ( 5 )   729 - 735   1993.7

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    Effects of the elicitor and/or suppressor from Mycosphaerella pinodes on polyphosphoinositide metabolism (PI metabolism) in pea were examined both in vivo and in vitro. The elicitor induced a rapid and biphasic increase in levels of phosphatidylinositol-4,5-bisphosphate (PtdInsP2) and inositol 1,4,5-trisphosphate (IP3) in epicotyl tissues that was apparent within 15 min. A transient increase in levels of PtdInsP2 and IP3 was detected immediately in elicitor-treated plasma membranes. However, the concomitant presence of suppressor with elicitor resulted in inhibition of these increases both in vivo and in vitro. These findings suggest that the elicitor rapidly activates phosphatidylinositol kinase, phosphatidylinositol-4-monophosphate kinase and phospholipase C, which are involved in PI metabolism, whereas the suppressor markedly inhibits these enzymes. Neomycin, a known inhibitor of phospholipase C, blocked the elicitor-induced accumulation both of IP3 and pisatin and it also induced local susceptibility in pea tissues that resembled that of the fungal suppressor. From these results, it appears that rapid changes in PI metabolism are indispensable in the signal transduction related to defense responses of pea plants.

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  • Inhibition of ATPase activity in pea plasma membranes by fungal suppressors from mycosphaerella pinodes and their peptide moieties Reviewed

    Toshiaki Kato, Tomonori Shiraishi, Kazuhiro Toyoda, Koji Saitoh, Yoshimi Satoh, Makoto Tahara, Tetsuji Yamada, Hachiro Oku

    Plant and Cell Physiology   34 ( 3 )   439 - 445   1993.4

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    The effects of two suppressors of the defense reactions of host plants, which had been purified from the pea pathogen Mycosphaerella pinodes, as well as the effects of peptide moieties, on the ATPase activity in pea plasma membranes were examined in vitro. One of the suppressors, Supprescin B, inhibited the ATPase activity in a non-competitive manner, but the other suppressor, Supprescin A, did not. Supprescin A was observed to reduce the inhibitory effect of Supprescin B. A tripeptide, Ser-Ser-Gly, and a hexapeptide, Ser-Ser-Gly-Asp-Glu-Thr, which were the respective peptide moieties of Supprescin A and B, inhibited the ATPase activity in a competitive manner. Supprescin B and fragments of the hexapeptide, such as Asp-Glu-Thr and Gly- Asp-Glu, inhibited not only the ATPase activity but also the acid phosphatase activity of plasma membranes in vitro. These results indicate that the acidic amino-acid residues of the "Asp-Glu" moiety seem to act as inhibitors of the phosphatase activity. Thus, the peptide moiety of Supprescin B consists of at least two functional elements. © 1993. The Japanese Society of Plant Physiologists (JSPP).

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  • Elicitor activity of a fungal product assessed at the single-cell level by a novel gel-bead method Reviewed

    Kazuhiro Toyoda, Issei Kobayashi, Hitoshi Kunoh

    Plant and Cell Physiology   34 ( 5 )   775 - 780   1993

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Oxford University Press  

    Elicitor from Erysiphe pisi was incorporated into gel beads. Individual beads were placed on single cells from barley coleoptiles. The elicitor induced unusual cytoplasmic responses and temporary resistance to infection in coleoptile cells. The technique is applicable to assessment of elicitor activity at the single-cell level. © 1993. The Japanese Society of Plant Physiologists (JSPP).

    DOI: 10.1093/oxfordjournals.pcp.a078483

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  • REGULATION OF POLYPHOSPHOINOSITIDE METABOLISM IN PEA PLASMA-MEMBRANES BY ELICITOR AND SUPPRESSOR FROM A PEA PATHOGEN, MYCOSPHAERELLA-PINODES Reviewed

    K TOYODA, T SHIRAISHI, H YOSHIOKA, T YAMADA, Y ICHINOSE, H OKU

    PLANT AND CELL PHYSIOLOGY   33 ( 4 )   445 - 452   1992.6

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    Effects of the elicitor and the suppressor from a pea pathogen, Mycosphaerella pinodes, on polyphosphoinositide metabolism in pea plasma membranes were examined in vitro. Lipid phosphorylation in the isolated pea plasma membrane was drastically stimulated by the elicitor, but markedly inhibited by the suppressor. A similar inhibitory effect was observed by the treatment with orthovanadate or K-252a that blocked pisatin production induced by the elicitor. Neomycin, an aminoglycoside antibiotic that interacts with the polyphosphoinositide metabolism, also affected the lipid phosphorylation in vitro and blocked the elicitor-induced accumulation of pisatin in vivo. These results suggest that rapid changes of polyphosphoinositide metabolism in pea plasma membranes is one of indispensable processes during the elicitation of defense responses.

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  • Effect of methyl jasmonate on harpin-induced hypersensitive cell heath, generation of hydrogen peroxide and expression of PAL mRNA in tobacco suspension cultured BY-2 cells Reviewed

    S Andi, F Taguchi, K Toyoda, T Shiraishi, Y Ichinose

    PLANT AND CELL PHYSIOLOGY   42 ( 4 )   446 - 449   1992.4

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    Methyl jasmonate inhibited the harpin-induced defense responses such as cell death, H2O2 generation and gene expression encoding phenylalanine ammonia-lyase in tobacco suspension cultured BY-2 cells, These results suggest that MeJA may act as an endogenous suppressor for plant defense response including hypersensitive reaction.

    DOI: 10.1093/pcp/pce056

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  • Induced accessibility and enhanced inaccessibility at the cellular level in barley coleoptiles IX. Accessibility induced by Erysiphe graminis which promotes the subsequent infection of challenging E.graminis.:Accessibility Induced by Erysiphe graminis which Promotes the Subsequent Infection of Challenging E. graminis

    KUNOH Hitoshi, KURODA Katsutoshi, TOYODA Kazuhiro, YAMAOKA Naoto, KOBAYASHI Issei

    Jpn. J. Phytopathol.   57 ( 1 )   57 - 60   1991

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    Language:English   Publisher:The Phytopathological Society of Japan  

    DOI: 10.3186/jjphytopath.57.57

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  • Induced accessibility and enhanced inaccessibility at the cellular level in barley coleoptiles. V. Duration of stimulus by a non-pathogen in relation to enhanced inaccessibility

    Hitoshi Kunoh, Kazuhiro Toyoda, Naoto Yamaoka, Issei Kobayashi

    Physiological and Molecular Plant Pathology   35 ( 6 )   507 - 518   1989.12

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    The influence of the duration of a direct stimulus by a non-pathogen to the enhancement of inaccessibility was examined using barley coleoptile cells and Erysiphe pisi. The degree of enhanced inaccessibility was evaluated with the rate of haustorium formation (penetration efficiency, PE) of E. graminis which attempted penetration of the same coleoptile cells as E. pisi. Partially dissected coleoptiles (coleoptiles A) were inoculated with E. pisi conidia. By observing the specimens every 15 min, the time of initiation of cytoplasmic aggregation in coleoptile cells below an individual E. pisi appressorium was recorded. At 0·5, 1·0 or 3·0 h after the initiation of cytoplasmic aggregation, germlings of E. pisi were removed by a micromanipulator. Germinating conidia of E. graminis which had been inoculated onto other coleoptiles were then transferred onto coleoptile A by micromanipulation so that they could attempt to penetrate the same cells in which E. pisi had induced a cytoplasmic aggregate. When E. pisi was removed 0·5 h after the initiation of cytoplasmic aggregation, the PE of E. graminis was reduced by 35·4%, suggesting that the inaccessibility was enhanced by the presence of E. pisi. When E. pisi was removed at 1·0 and 3·0 h, the subsequent PEs of E. graminis were 24·8% and 5·8%, respectively, indicating that the prolonged presence of E. pisi further enhanced the inaccessibility. The degree of enhanced inaccessibility was influenced by the size of coleoptile cells but not by the distances between penetration sites of the two fungi within the same cell and the time intervals of the initiation of cytoplasmic aggregation induced by both fungi. It is suggested that unknown mechanism(s) unrelated to papilla formation may be involved in the enhancement of inaccessibility. © 1989.

    DOI: 10.1016/0885-5765(89)90092-1

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Books

  • Resistance and susceptibility of plants to fungal pathogens

    Transgenic Research  2002 

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  • Regulation of nuclear gene expression in relation to signal molecules.

    Delivery of Pathogen Signals to Plants(N. T. Keen Ed. )APS Press, St. Paul Minnesota, USA  2001 

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  • Suppressors of defense-Supprescins and Plant receptor molecules.

    Delivery of Pathogen Signals to Plants(N. T. Keen Ed. )APS Press, St. Paul Minnesota, USA  2001 

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  • 宿主特異性決定における植物細胞壁の役割

    東北地方における植物病理学のフロントライン(日本植物病理学会東北部会)  2000 

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  • Suppressor as a factor determining plant-pathogen specificity

    Plant-Microbe Interactions Vol. 4(Stacey, G. and Ken, N. T. eds. ), APS Press, St. Paul Minnesota, USA  1999 

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  • Fungal Signals Regulate ATPase and polyphosphoinositide me Tabolism in Pea(共著)

    Molecular Aspects of Pathogenicity and Host Resistance : Requirement for signal Transduction(Ed. by D. Mills etal)American Phytopathological Society  1996 

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  • Early signaling events required for induction of phytoalexin accumulation in pea epiocotyls:phosphoinositides and their role in signal transduction

    China Agricultural Scientech Press 

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  • Early signaling events required for induction of phytoalexin accumulation in pea epiocotyls:phosphoinositides and their role in signal transduction

    China Agricultural Scientech Press 

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MISC

  • Induction of CEP genes in Arabidopsis in response to pathogen infection and salicylic acid.

    伊藤千晶, 長谷川晴香, APRILIA Nur Fitrianti, 松井英譲, 山本幹博, 能年義輝, 一瀬勇規, 白石友紀, 豊田和弘

    日本植物病理学会報   89 ( 1 )   2023

  • Towards comprehensive identification of host factors targeted by Pta type III effectors.

    黒江香那, 樫原沙知, 西村隆史, 能年義輝, 山本幹博, 豊田和弘, 中神弘史, 一瀬勇規, 松井英譲

    日本植物病理学会報   89 ( 1 )   2023

  • Functional analysis of cytosolic chemoreceptor in virulence of Pseudomonas syringae pv. tabaci 6605.

    渡邊雄太, STEPHANY Angelia Tumewu, 松井英譲, 山本幹博, 能年義輝, 豊田和弘, 一瀬勇規

    日本植物病理学会報   89 ( 1 )   2023

  • MAMP-responsive phosphoprotein MARK2 contributes to disease resistance against Colletotrichum higginsianum.

    松井英讓, 松井英讓, 澁谷日奈, 太田和輝, 前田和輝, 晝間敬, 四井いずみ, 四井いずみ, HYON G-S., 野村有子, 能年義輝, 豊田和弘, 一瀬勇規, 中神弘史, 中神弘史

    日本植物病理学会報   88 ( 1 )   2022

  • Study on the mode of action of metominostrobin as a plant activator 7. Effects on expression of PTI- and SAR-related genes PTI in Arabidopsis.

    伊藤千晶, 小原七海, 佐藤穂高, 市成光広, 山田晶, 櫻本和生, 白石慎, 山本隆, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会報   88 ( 1 )   2022

  • Rhizoviticin, a tailocin, as a determinant of biocontrol activity of Rhizobium vitis VAR03-1 towards grapevine crown gall disease

    土田菜月, 石井智也, 渡邉恵, 齊藤昌, BAO Jiyuan, HEMELDA Merry Niarsi, 佐藤繭子, 豊岡公徳, 石濱伸明, 白須賢, 豊田敦, 松原岳大, 松井英譲, 松井英譲, 山本幹博, 山本幹博, 豊田和弘, 豊田和弘, 一瀬勇規, 一瀬勇規, 川口章, 能年義輝, 能年義輝

    日本植物病理学会大会プログラム・講演要旨予稿集   2022   2022

  • Contribution of cell wall peroxidase and NADPH oxidase to MAMP-induced immunity in Arabidopsis.

    木元菜々子, 高須瑞穂, FITRIANTI Aprilia Nur, 松井英讓, 松井英讓, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘

    日本植物病理学会報   88 ( 1 )   2022

  • Characterization of rhizoviticin, a phage tail-like particle, as a determinant of biocontrol activity of Rhizobium vitis VAR03-1 on grapevine crown gall disease.

    土田菜月, 石井智也, 渡邉恵, 齊藤晶, BAO J., 佐藤繭子, 豊岡公徳, 石濱伸明, 白須賢, 豊田敦, 松原岳大, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

    日本植物病理学会報   88 ( 1 )   2022

  • Colonization ability of Rhizobium vitis VAR03-1, a biocontrol agent for grapevine crown gall disease, in Arabidopsis thaliana.

    BAO J., 松井英讓, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

    日本植物病理学会報   88 ( 1 )   2022

  • A study of the host factors targeted by Pseudomonas syringae pv. tabaci type III effector J.

    樫原沙知, 西村隆史, 中神弘史, 能年義輝, 山本幹博, 豊田和弘, 一瀬勇規, 松井英譲

    日本植物病理学会報   88 ( 1 )   2022

  • Upregulation of CEP genes in Arabidopsis leaves challenged with avirulent and non-adapted bacteria

    APRILIA Nur Fitrianti, 伊藤千晶, 長谷川晴香, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2022   2022

  • Towards a comprehensive identification of host factors targeted by Type III effectors of the tobacco wildfire

    黒江香那, 樫原沙知, 西村隆史, 能年義輝, 山本幹博, 豊田和弘, 中神弘史, 一瀬勇規, 松井英譲

    日本植物病理学会大会プログラム・講演要旨予稿集   2022   2022

  • Functional analysis of the plant immune signaling pathway mediated by MAMP-responsive Raf-like protein kinase 1 (MRPK1) in Arabidopsis.

    藤山祐香, 松井英譲, 松井英譲, HYON G.-S., 野村有子, 能年義輝, 豊田和弘, 一瀬勇規, 中神弘史, 中神弘史

    日本植物病理学会報   88 ( 1 )   2022

  • Functional analysis of type III effector J protein of Psedomonas syringae pv. tabaci 6605

    樫原沙知, 西村隆史, 能年義輝, 山本幹博, 豊田和弘, 中神弘史, 一瀬勇規, 松井英譲

    日本植物病理学会大会プログラム・講演要旨予稿集   2022   2022

  • CEP peptides, a family of conserved, secreted peptides that negatively modulate Arabidopsis immunity: III. Potential role during PTI and ETI

    伊藤千晶, 長谷川晴香, FITRIANTI Aprilia Nur, 松井英譲, 松井英譲, 山本幹博, 山本幹博, 能年義輝, 能年義輝, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

    植物微生物研究会研究交流会講演要旨集   31st   2022

  • Colonization mechanism of Rhizobium vitis VAR03-1, a biocontrol agent for grapevine crown gall disease, on Arabidopsis thaliana

    BAO Jiyuan, HEMELDA Niarsi Merry, 土田菜月, 渡邉恵, 松井英譲, 松井英譲, 山本幹博, 山本幹博, 豊田和弘, 豊田和弘, 一瀬勇規, 一瀬勇規, 川口章, 能年義輝, 能年義輝

    日本植物病理学会大会プログラム・講演要旨予稿集   2022   2022

  • CEP peptides, a family of conserved, secreted peptides that negatively modulate Arabidopsis immunity: IV. CEP peptide attenuates SA-mediated immunity

    長谷川晴香, 伊藤千晶, FITRIANTI Aprilia Nur, 松井英譲, 松井英譲, 山本幹博, 山本幹博, 能年義輝, 能年義輝, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

    植物微生物研究会研究交流会講演要旨集   31st   2022

  • Involvement of cell wall peroxidase and NADPH oxidase in MAMP-induced oxidative burst in Arabidopsis.

    木元菜々子, 高須瑞穂, APRILIA Nur Fitrianti, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    日本植物病理学会報   87 ( 1 )   2021

  • A possible role of an NADPH oxidase RBOHD in regulating intra/extracellular redox in Arabidopsis thaliana.

    高須瑞穂, 木元菜々子, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    日本植物病理学会報   87 ( 1 )   2021

  • The involvement of chemoreceptor gene mcp on virulence in Pseudomonas syringae pv. tabaci 6605

    寺元茜, 金重慎, TUMEWU S.A., 松井英譲, 山本幹博, 能年義輝, 豊田和弘, 一瀬勇規

    日本植物病理学会大会プログラム・講演要旨予稿集   2021   2021

  • Study on the Mode of Action of Metominostrobin as a Plant Activator 6. Effect on Expression of PTI-related Genes in Arabidopsis

    小原七海, 伊藤千晶, 佐藤穂高, 市成光広, 山田晶, 櫻本和生, 白石慎, 山本隆, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2021   2021

  • Study on the mode of action of metominostrobin as a plant activator 3. Metominostrobin causes sustained activation of MAP kinases in Arabidopsis immunity.

    伊藤千晶, 小原七海, 佐藤穂高, 市成光広, 山田晶, 櫻本和生, 白石慎, 山本隆, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

    日本農薬学会大会講演要旨集   46th (CD-ROM)   2021

  • Recognition of pathogenic fungi by unknown receptor(s) in Arabidopsis thaliana.

    矢野裕奈, APRILIA Nur Fitrianti, 木元菜々子, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    日本植物病理学会報   87 ( 1 )   2021

  • Search for virulence factors in Pseudomonas syringae pv. tabaci using comparative genomic analysis

    吉岡桂, 西村隆史, 浅井秀太, 増田幸子, 白須賢, 山本幹博, 能年義輝, 豊田和弘, 一瀬勇規, 松井英譲

    日本植物病理学会大会プログラム・講演要旨予稿集   2021   2021

  • Study on the Mode of Action of Metominostrobin as a Plant Activator 5. Effect on MAP Kinases During PTI in Arabidopsis

    伊藤千晶, 小原七海, 佐藤穂高, 市成光広, 山田晶, 櫻本和生, 白石慎, 山本隆, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2021   2021

  • Identification of effectors contributing to the development of disease symptom in Tobacco wildfire pathogens.

    西村隆史, 樫原沙知, 能年義輝, 山本幹博, 豊田和弘, 一瀬勇規, 松井英譲

    日本植物病理学会報   87 ( 1 )   2021

  • Study on the mode of action of metominostrobin as a plant activator 4. Metominostrobin enhances expression of PTI-related genes in Arabidopsis.

    小原七海, 伊藤千晶, 佐藤穂高, 市成光広, 山田晶, 櫻本和生, 白石慎, 山本隆, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

    日本農薬学会大会講演要旨集   46th (CD-ROM)   2021

  • Tobacco wildfire-resistant cultivars recognize the Pta TTSS effector and induce HR.

    樫原沙知, 西村隆史, 能年義輝, 山本幹博, 豊田和弘, 一瀬勇規, 松井英譲

    日本植物病理学会報   87 ( 1 )   2021

  • Molecular mechanism on MAMP-triggered ROS burst in Arabidopsis

    木元菜々子, 高須瑞穂, FITRIANTI Aprilia Nur, 松井英讓, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    植物微生物研究会研究交流会講演要旨集   30th   2021

  • Study on the Mode of Action of Metominostrobin as a Plant Activator: 8. Effects on ROS burst, MAPK activation and expression of defense-related genes in Arabidopsis.

    伊藤千晶, 小原七海, 佐藤穂高, 市成光広, 山田晶, 櫻本和生, 白石慎, 山本隆, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

    植物微生物研究会研究交流会講演要旨集   30th   2021

  • Regulation of plant immunity by pathogen- and plant-derived suppressors.

    豊田和弘, 豊田和弘, 豊田和弘, APRILIA Nur Fitrianti, MAI Thanh Luan, 川崎達弘, 門田日陽里, 椎葉紀香, 伊藤千晶, 長谷川晴香, 白石友紀

    日本植物病理学会植物感染生理談話会論文集   ( 55 )   2021

  • Prerequisites for the oxidative burst in Arabidopsis: A role of NADPH oxidase, RBOHD in regulating intra/extracellular redox

    高須瑞穂, 木元菜々子, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020

  • c-di-GMP-related gene 1 (CDG1) contributes to the regulation of virulence in Pta 6605

    澤田春那, 岡本卓巳, 能年義輝, 山本幹博, 豊田和弘, 松井英譲, 一瀬勇規

    日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020

  • Pseudomonas syringae pv. tabaci effectors that contribute to the development of disease symptom

    西村隆史, 樫原沙知, 能年義輝, 山本幹博, 豊田和弘, 一瀬勇規, 松井英譲

    日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020

  • Wildfire disease resistant tobacco recognizes type III secretion effector of Pseudomonas syringae pv. tabaci

    樫原沙知, 西村隆史, 能年義輝, 山本幹博, 豊田和弘, 一瀬勇規, 松井英譲

    日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020

  • Studies on the mode of action of metominostrobin II. Priming against powdery mildew resistance in wheat.

    佐藤穂高, PHUONG Le Thi, 市成光広, 櫻本和生, 山田晶, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    日本植物病理学会報   86 ( 1 )   2020

  • Recognition of fungal pathogen in Arabidopsis, that does not require a known receptor

    矢野裕奈, APRILIA NUR F., 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020

  • A tailocin responsible for antagonistic activity of Rhizobium vitis VAR03-1 against grapevine crown gall disease

    石井智也, 齋藤晶, 渡邉恵, 佐藤繭子, 豊岡公徳, 石濱伸明, 白須賢, BAO J., 松井英譲, 山本幹博, 豊田和弘, 一瀬勇規, 川口章, 能年義輝

    日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020

  • Contribution of cell wall peroxidase and NADPH oxidase to the MAMP-induced oxidative burst in Arabidopsis

    木元菜々子, 高須瑞穂, APRILIA NUR F., ZHAO L., LE THI P., 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2020   2020

  • Immunity response of Brachypodium distachyon induced by microbe-associated molecular patterns.

    小笠原翼, 香西雄介, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 持田恵一, 能年義輝

    日本植物病理学会報   86 ( 1 )   2020

  • Arabidopsis class III peroxidase PRX34 plays a role in the MAMP-elicited oxidative burst.

    高須瑞穂, 木元菜々子, LEI Zhao, PHUONG Le Thi, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会報   86 ( 1 )   2020

  • Kinetics study of Arabidopsis salicylic acid glucosyltransferase inhibitors as potential plant activators.

    篠原優佳, 渡邉恵, 楠和輝, 谷川友里佳, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 熊谷和夫, 米須清明, 能年義輝

    日本植物病理学会報   86 ( 1 )   2020

  • Search of major virulence factors of Pseudomonas syringae pv. tabaci.

    西村隆史, 樫原沙知, 山本幹博, 能年義輝, 豊田和弘, 一瀬勇規, 松井英譲

    日本植物病理学会報   86 ( 1 )   2020

  • Quorum-dependent expression of rsmX and rsmY, small non-coding RNAs, in Pseudomonas syringae

    Nakatsu Yukiko, Matsui Hidenori, Yamamoto Mikihiro, Noutoshi Yoshiteru, Toyoda Kazuhiro, Ichinose Yuki

    Microbiological Research   223-225   72‐78 - 78   2019

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    Pseudomonas syringae pathovars are known to produce N-acyl-homoserine lactones (AHL) as quorum-sensing molecules. However, many isolates, including P. syringae pv. tomato DC3000 (PtoDC3000), do not produce them. In P. syringae, psyI, which encodes an AHL synthase, and psyR, which encodes the transcription factor PsyR required for activation of psyI, are convergently transcribed. In P. amygdali pv. tabaci 6605 (Pta6605), there is one nucleotide between the stop codons of both psyI and psyR. However, the canonical stop codon for psyI in PtoDC3000 was converted to the cysteine codon by one nucleotide deletion, and 23 additional amino acids extended it to a C-terminal end. This resulted in overlapping of the open reading frame (ORF) for psyI and psyR. On the other hand, stop codons in the psyR ORF of P. syringae 7 isolates, including pv. phaseolicola and pv. glycinea, were found. These results indicate that many pathovars of P. syringae have genetically lost AHL production ability by the mutation of their responsible genes. To examine whether PtoDC3000 modulates the gene expression profile in a population-dependent manner, we carried out microarray analysis using RNAs prepared from low- and high-density cells. We found the expressions of rsmX and rsmY remarkably activated in highdensity cells. The activated expressions of rsmX and rsmY were confirmed by Northern blot hybridization, but these expressions were abolished in a.gacA mutant of Pta6605. These results indicate that regardless of the ability to produce AHL, P. syringae regulates expression of the small noncoding RNAs rsmX/Y by currently unknown quorum-sensing molecules.

    DOI: 10.1016/j.micres.2019.04.004

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  • 微生物分子パターンに対するミナトカモジグサの活性酸素種生成特性の解析

    小笠原翼, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 香西雄介, 能年義輝

    日本植物病理学会報   85 ( 1 )   2019

  • Pseudomonas syringae pv.tabaciのRNAタイプ多剤排出トランスポーターの病原力における機能解析

    西村隆史, 一瀬勇規, 原田みのり, 松井英譲, 山本幹博, 能年義輝, 豊田和弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2019   2019

  • 非病原性Rhizobium vitis VAR03-1株によるブドウ根頭がんしゅ病の病原性関連遺伝子の発現抑制機構の解析

    石井智也, 渡邉恵, 齊藤晶, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

    日本植物病理学会報   85 ( 1 )   2019

  • メトミノストロビンの作用機構に関する研究

    佐藤穂高, 市成光広, 櫻本和生, 山田晶, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    日本植物病理学会報   85 ( 1 )   2019

  • タバコ野火病菌のCDG1はアシルホモセリンラクトンの蓄積を正に制御する

    澤田春那, 岡本卓巳, 能年義輝, 山本幹博, 豊田和弘, 松井英譲, 一瀬勇規

    日本植物病理学会報   85 ( 1 )   2019

  • 抵抗性誘導剤のシーズとして単離したサリチル酸配糖化酵素阻害剤の反応速度論解析

    篠原優佳, 渡邉恵, 楠和輝, 谷川友里佳, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 熊谷和夫, 米須清明, 能年義輝

    日本植物病理学会報   85 ( 1 )   2019

  • エンドウの細胞壁タンパク質に検出されるキトサン結合タンパク質

    松尾実佳, 川端真矢, 三木紅葉, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    日本植物病理学会報   85 ( 1 )   2019

  • エンドウの細胞壁画分に見出されたキトサンと結合するタンパク質複合体

    松尾実佳, 三木紅葉, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2019   2019

  • 細胞外オキシダティブバースト反応の分子機構:ペルオキシダーゼのスルフェン酸化とその役割

    高須瑞穂, 吉岡美樹, 吉岡博文, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    日本植物病理学会報   85 ( 1 )   2019

  • 植物免疫活性剤インプリマチンDがpattern-triggered immunityに与える影響

    山枡一秀, 平田奈美, 福井絢子, 渡邉恵, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 能年義輝

    日本植物病理学会大会プログラム・講演要旨予稿集   2019   2019

  • Pseudomonas syringaeの菌体密度感知機構の解析(2)

    一瀬勇規, 松井英譲, 石賀康博, 山本幹博, 能年義輝, 豊田和弘

    日本植物病理学会報   84 ( 1 )   2018

  • Pseudomonas syringaeの菌体密度感知機構の解析(4)菌体密度に応じた遺伝子発現制御

    中津有紀子, 松井英譲, 山本幹博, 能年義輝, 豊田和弘, 一瀬勇規

    日本植物病理学会大会プログラム・講演要旨予稿集   2018   2018

  • バイオコントロール細菌Rhizobium vitis VAR03-1株のブドウ根頭がんしゅ病抑制機構の解析

    齊藤晶, 渡邉恵, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

    日本植物病理学会大会プログラム・講演要旨予稿集   2018   2018

  • Pseudomonas syringaeの菌体密度感知機構の解析(3)PsyRとアシルホモセリンラクトンによる遺伝子発現制御機構

    田阪洋昌, 山本悟, 松井英譲, 山本幹博, 能年義輝, 豊田和弘, 一瀬勇規

    日本植物病理学会大会プログラム・講演要旨予稿集   2018   2018

  • エンドウの細胞壁タンパク質に検出されるキトサン結合タンパク質

    松尾実佳, 川端真矢, 三木紅葉, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    植物微生物研究会研究交流会講演要旨集   28th   2018

  • ササゲにおける細胞外オキシダティブバースト反応の分子機構

    川端真矢, 佐藤穂高, 高須瑞穂, 松尾実佳, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会報   84 ( 1 )   2018

  • 非病原性Rhizobium vitis VAR03-1株の培養上清がブドウ根頭がんしゅ病菌に及ぼす影響

    齊藤晶, 渡邉恵, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

    日本植物病理学会報   84 ( 1 )   2018

  • 細菌シグナル伝達因子CDG proteinの機能解析

    澤田春那, 岡本卓巳, 能年義輝, 山本幹博, 豊田和弘, 松井英譲, 一瀬勇規

    日本植物病理学会大会プログラム・講演要旨予稿集   2018   2018

  • デュアル抵抗性蛋白質システムを構成するRRS1およびRPS4の機能解析

    鳴坂真理, 白須賢, 豊田和弘, 高野義孝, 白石友紀, 鳴坂義弘

    日本生化学会大会(Web)   90th   ROMBUNNO.3P‐1257 (WEB ONLY)   2017

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  • エンドウから分離した細胞壁タンパク質のPAMP応答

    三木紅葉, 山崎史織, 片岡千香子, 松尾実佳, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会報   83 ( 1 )   2017

  • Pseudomonas syringae pv.tabaci6605におけるAHL合成制御機構並びにMexEFOprN多剤排出ポンプの病原力における役割の解析

    澤田貴博, 山本幹博, 松井英譲, 能年義輝, 豊田和弘, 一瀬勇規

    日本植物病理学会報   83 ( 1 )   2017

  • シロイヌナズナにおけるPAMP誘導性オキシダティブバースト反応におけるアポプラストの役割について

    片岡千香子, 山崎史織, 松尾実佳, 三木紅葉, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会報   83 ( 1 )   2017

  • シロイヌナズナにおける細胞外オキシダティブバースト反応の分子機構

    松尾実佳, 片岡千香子, 山崎史織, 三木紅葉, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会報   83 ( 1 )   2017

  • 非病原性Rhizobium vitis ARK-1株によるブドウ根頭がんしゅ病の病原性関連遺伝子の発現抑制

    齊藤晶, 渡邉恵, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

    日本植物病理学会報   83 ( 1 )   2017

  • エクト型ATPase(PsAPY1)はエンドウ葉におけるPAMP誘導性オキシダティブバースト反応を正に調節し,不適応型の病原菌に対する非宿主抵抗性に関与する

    山崎史織, 三木紅葉, 矢尾幸世, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会報   83 ( 1 )   2017

  • Pseudomonas syringaeの菌体密度感知機構の解析(1)

    山本悟, 藤山友里, 高田基弘, 松井英譲, 山本幹博, 能年義輝, 豊田和弘, 一瀬勇規

    日本植物病理学会報   83 ( 1 )   2017

  • 非病原性Rhizobium vitis VAR03-1株によるブドウ根頭がんしゅ病の病原性関連遺伝子の発現抑制

    齊藤晶, 渡邉恵, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

    日本植物病理学会大会プログラム・講演要旨予稿集   2017   2017

  • エンドウならびにシロイヌナズナにおけるPAMP誘導性細胞外オキシダティブバースト反応の分子機構

    片岡千香子, 三木紅葉, 山崎史織, 松尾実佳, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2017   2017

  • PAMP誘導性細胞外オキシダティブバースト反応の分子機構

    川端真矢, 佐藤穂高, 高須瑞穂, 松尾実佳, 松井英譲, 松井英譲, 能年義輝, 能年義輝, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

    植物微生物研究会研究交流会講演要旨集   27th   2017

  • ミナトカモジグサにおける植物免疫ホルモン応答性マーカー遺伝子の同定と発現プロファイルの解析

    香西雄介, 山中由利恵, 渡邉恵, 木村麻美子, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 恩田義彦, 持田恵一, 能年義輝

    日本植物病理学会報   83 ( 1 )   2017

  • デュアル抵抗性蛋白質システムを構成する抵抗性蛋白質RRS1の機能解析

    鳴坂真理, 白須賢, 豊田和弘, 高野義孝, 白石友紀, 鳴坂義弘

    日本植物細胞分子生物学会大会・シンポジウム講演要旨集   34th   145   2016.8

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  • デュアル抵抗性蛋白質システムを構成する抵抗性蛋白質RRS1のロイシンジッパーモチーフの機能解析

    鳴坂真理, 白須賢, 豊田和弘, 高野義孝, 白石友紀, 鳴坂義弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2016   90   2016.3

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  • デュアル抵抗性蛋白質を構成するRPS4およびRRS1の機能解析

    鳴坂真理, 白須賢, 豊田和弘, 高野義孝, 白石友紀, 鳴坂義弘

    日本植物病理学会報   82 ( 1 )   68(J‐STAGE)   2016

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  • Pseudomonas syringae pv.syringae B728aの病原力制御因子Vfrの標的遺伝子のクロマチン免疫沈降法による探索(2)

    小倉敬右, 田口富美子, 山本幹博, 松井英譲, 能年義輝, 豊田和弘, 一瀬勇規

    日本植物病理学会大会プログラム・講演要旨予稿集   2016   2016

  • エクト型ATPase(PsAPY1)はエンドウ葉におけるリポ多糖(LPS)誘導性細胞外オキシダティブバースト反応を正に調節する

    三木紅葉, 山崎史織, 矢尾幸世, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 白石友紀, 豊田和弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2016   2016

  • ナス科植物青枯病菌の病原力や増殖を制御する化合物の探索

    澤井拓, 一瀬勇規, 山本幹博, 松井英譲, 能年義輝, 豊田和弘, 向原隆文, 長田裕之

    日本植物病理学会大会プログラム・講演要旨予稿集   2016   2016

  • 非病原性Rhizobium vitis ARK-1株によるブドウ根頭がんしゅ病の発病発現抑制機構の解析

    齊藤晶, 渡邉恵, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝

    日本植物病理学会大会プログラム・講演要旨予稿集   2016   2016

  • エクト型ATPase(PsAPY1)の発現を抑制したエンドウ葉におけるジャスモン酸応答性遺伝子の発現

    矢尾幸世, 田中佳織, 奥田竜太, 三木紅葉, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 白石友紀, 豊田和弘

    日本植物病理学会報   82 ( 1 )   2016

  • ミナトカモジグサ-紋枯病菌感染系の確立と植物ホルモンによる抵抗性機構の解析

    香西雄介, 山中由利恵, 渡邉恵, 木村麻美子, 恩田義彦, 持田恵一, 山本幹博, 松井英譲, 一瀬勇規, 豊田和弘, 能年義輝

    日本植物病理学会報   82 ( 1 )   2016

  • Pseudomonas syringae pv.tabaciにおけるAHL合成制御機構の解析

    澤田貴博, 山本幹博, 松井英譲, 能年義輝, 豊田和弘, 一瀬勇規

    日本植物病理学会報   82 ( 1 )   2016

  • サリチル酸はミナトカモジグサの紋枯病菌に対する侵入後抵抗性を高める

    香西雄介, 木村麻美子, 渡邉恵, 山中由利恵, 恩田義彦, 持田恵一, 一瀬勇規, 山本幹博, 松井英譲, 豊田和弘, 能年義輝

    日本植物病理学会大会プログラム・講演要旨予稿集   2016   2016

  • 抵抗性誘導剤の同定を目的としたサリチル酸配糖化酵素阻害剤の標的ベース探索

    楠和輝, 渡邉恵, 米須清明, 熊谷和夫, 香西雄介, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 能年義輝

    日本植物病理学会大会プログラム・講演要旨予稿集   2016   2016

  • デュアル抵抗性蛋白質システムを構成するRPS4およびRRS1の機能解析

    鳴坂真理, 白須賢, 豊田和弘, 白石友紀, 鳴坂義弘

    日本植物生理学会年会要旨集   56th   339   2015.3

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  • デュアル抵抗性蛋白質RPS4およびRRS1をコアとする蛋白質複合体の機能解析

    鳴坂真理, 白須賢, 豊田和弘, 白石友紀, 鳴坂義弘

    日本植物病理学会報   81 ( 1 )   68   2015

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  • イネにおける異種β-アミリン酸化酵素遺伝子の過剰発現

    内藤優, 島谷善平, 島谷善平, 青野裕子, 平井宏太, 寺田理枝, OSBOURN Anne, 關光, 松島良, 能年義輝, 能年義輝, 豊田和弘, 豊田和弘, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 稲垣善茂, 稲垣善茂, 稲垣善茂

    イソプレノイド研究会例会講演要旨集   25th   2015

  • 異種β-アミリン酸化酵素遺伝子を過剰発現した組換え体イネにおける生理学的影響

    内藤優, 島谷善平, 島谷善平, 青野裕子, 平井宏太, 寺田理枝, OSBOURN Anne, 關光, 松島良, 能年義輝, 豊田和弘, 山本幹博, 一瀬勇規, 稲垣善茂

    日本植物生理学会年会要旨集   56th   2015

  • エクト型ATPase(PsAPY1)の発現を抑制したエンドウの非適応型アブラナ科炭疽病菌に対する応答

    矢尾幸世, 三木紅葉, 鈴木智子, 山岸紀子, 吉川信幸, 能年義輝, 能年義輝, 稲垣善茂, 稲垣善茂, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2015   2015

  • デュアル抵抗性蛋白質システムにおける蛋白質間相互作用の解析 2

    鳴坂真理, 白須賢, 豊田和弘, 白石友紀, 高野義孝, 久保康之, 鳴坂義弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2014   61   2014.5

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  • デュアル抵抗性蛋白質システムにおけるタンパク質間相互作用の解析

    鳴坂真理, 白須賢, 豊田和弘, 白石友紀, 鳴坂義弘

    日本植物生理学会年会要旨集   55th   320   2014.3

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  • エクト型ATPase(PsAPY1)高発現タバコに付与される耐病性の分子機構

    中桐萌, 木場章範, 能年義輝, 能年義輝, 稲垣善茂, 稲垣善茂, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2014   2014

  • エクト型ATPase(PsAPY1)の発現を抑制したエンドウの褐紋病菌に対する応答

    矢尾幸世, 高木麻衣, 田中佳織, 山岸紀子, 吉川信幸, 能年義輝, 能年義輝, 稲垣善茂, 稲垣善茂, 山本幹博, 山本幹博, 一瀬勇規, 一瀬勇規, 白石友紀, 白石友紀, 白石友紀, 豊田和弘, 豊田和弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2014   2014

  • Suppression of mRNAs for lipoxygenase (LOX), allene oxide synthase (AOS), allene oxide cyclase (AOC) and 12-oxo-phytodienoic acid reductase (OPR) in pea reduces sensitivity to the phytotoxin coronatine and disease development by Mycosphaerella pinodes

    TOYODA Kazuhiro, KAWANISHI Yuriko, KAWAMOTO Yuriko, KURIHARA Chiaki, YAMAGISHI Noriko, TAMURA Akihiro, YOSHIKAWA Nobuyuki, INAGAKI Yoshishige, ICHINOSE Yuki, SHIRAISHI Tomonori

    79 ( 5 )   321 - 334   2013.9

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  • 新説デュアル抵抗性蛋白質システムの作用機作の解明

    鳴坂真理, 豊田和弘, 白須賢, 白石友紀, 鳴坂義弘

    日本分子生物学会年会プログラム・要旨集(Web)   36th   1P-0488 (WEB ONLY)   2013

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  • 3S-Dp02 New Research Approach of Iron-oxidizing Bacterium, Gallionella ferruginea

    Suzuki Tomoko, Hashimoto Hideki, Ishihara Hiromichi, Matsumoto Nobuyuki, Akae Yuta, Toyoda Kazuhiro, Shiraishi Tomonori, Kunoh Hitoshi, Takada Jun

    65   181 - 181   2013

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  • 細胞外ATPによるオキシダティブバースト反応の制御

    田中佳織, 豊田和弘, 稲垣善茂, 一瀬勇規, 白石友紀

    日本植物病理学会報   79 ( 1 )   2013

  • ササゲにおけるペルオキシダーゼ依存性細胞外オキシダティブバースト反応の分子機構

    茶谷真菜美, 大河摩祐, 田中佳織, 稲垣善茂, 一瀬勇規, 豊田和弘, 白石友紀

    日本植物病理学会大会プログラム・講演要旨予稿集   2013   2013

  • シロイヌナズナ葉に存在する内生サプレッサー

    川崎達弘, 安倍幸子, 能年義輝, 稲垣善茂, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘, 豊田和弘

    植物微生物研究会研究交流会講演要旨集   23rd   2013

  • A volatile substance from Talaromyces sp promotes the plant growth and blocks the disease development on several plants

    T. Shiraishi, Y. Yamagiwa, P. T. Le, K. Maeda, Y. Inagaki, Y. Ichinose, M. Hyakumachi, K. Toyoda

    PHYTOPATHOLOGY   101 ( 6 )   S165 - S166   2011.6

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  • Metabolic analysis of transgenic rice introduced Avena strigosa beta-Amyrin Shynthase 1

    Ikeda Kousuke, Geisler Katrin, Aono Yuuko, Osbourn Anne, Toyoda Kazuhiro, Shiraishi Tomonori, Ichinose Yuki, Inagaki Yoshishige

    Plant and Cell Physiology Supplement   2011   0763 - 0763   2011

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    Triterpenoid saponins are sugar-modified triterpene derivatives that have various functions, anti-cancer, anti-oxidant, and anti-microbial. Cereals and grasses are generally deficient in this secondary metabolite except for oats. Our final goal is that the anti-microbial saponin is available for the rice. We reported already that 1) we found twelve Oxidosqualene cyclase homologues in rice genome using knowledge in oats, but it was likely that no one was involved in the triterpenoid biosynthesis pathway, 2) we also found twelve CYP51 sterol demethylase homologues in rice genome. It was likely that one is for rice steroids biosynthesis pathway, and another is for rice triterpeneoids biosynthesis pathway. Here, we report that 1) we made several transgenic rice lines introduced Avena strigosa beta-Amyrin Synthase 1 gene, 2) analyzed the triterpenoids and their intermediates extracted from the transgenic lines by GC/MS, 3) and demonstrated the accumulation of only beta-Amyrin in the transgenic rice without the other intermediates and/or the triterpenoids.

    DOI: 10.14841/jspp.2011.0.0763.0

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  • デュアルR‐遺伝子システムによる病原菌認識機構の解明

    鳴坂真理, 白須賢, 久保康之, 豊田和弘, 白石友紀, 岩渕雅樹, 鳴坂義弘

    日本植物病理学会大会プログラム・講演要旨予稿集   2010   81   2010.3

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  • Effect of FFC ceramic water on the infection process of a fungal pathogen

    Scientific Reports of the Faculty of Agriculture, Okayama University   99   27 - 34   2010.2

  • Enhancement of growth and yield of barley by the soil conditioner FFC-ace

    Scientific Reports of the Faculty of Agriculture, Okayama University   99   13 - 20   2010.2

  • Bacterial DNA activates immunity in Arabidopsis thaliana.

    Yakushiji, S, Ishiga, Y, Inagaki, Y, Toyoda, K, Shiraishi, T, Ichinose, Y

    J. Gen. Plant Pathol.   75 ( 3 )   227 - 234   2009

  • Molecular analysis for CYP51 sterol demethylase in rice

    Inagaki Yoshishige, Toyoda Kazuhiro, Shiraishi Tomonori, Ichinose Yuki, Osbourn Anne

    Plant and Cell Physiology Supplement   2009   0206 - 0206   2009

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    Triterpenoid saponins are suger-modified triterpene derivatives that have various functions, anti-cancer, anti-oxidant, and anti-microbial. Cereals and grasses are generally deficient in this secondary metabolite with the exception of oats. Our final goal is that the anti-microbial saponin is available for rice. We reported already that 1) triterpenoid saponins and the intermediates were not detected in rice young seedling by LC/MS and GC/MS analyses, 2) we found twelve Oxidosqualene cyclase homologues in rice genome using knowledge in oats, but it was likely that no one was involved in the triterpeneoid biosynthesis pathway. Here, we report that we found twelve OsCYP51 homologues in rice genome that are in next step of the pathway, and it is likely that one is for rice steroids biosynthesis pathway, and another is for rice triterpeneoids biosynthesis pathway, by genome structure and gene expression analyses.

    DOI: 10.14841/jspp.2009.0.0206.0

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  • (129) Molecular Analysis for Oxidosqualene Cyclase in Saponin Biosynthesis Pathway of Rice(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Inagaki Y., Imaoka A., Fujita K., Arase S., Toyoda K., Shiraishi T., Ichinose Y., Osbourn Anne

    Annals of the Phytopathological Society of Japan   74 ( 3 )   201 - 201   2008.8

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  • (127) Functional Analysis of Cell Wall-associated apyrase MtAPY1; 1 in Medicago truncatula(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Shiobara T., Fujita K., Inagaki Y., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   74 ( 3 )   200 - 200   2008.8

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  • (307) Functional Analysis of Flagellar Motility and Elicitor Activity of Flagellin in Pseudomonas syringae pv. tabaci(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Ichinose Y., Naito K., Taguchi F., Suzuki T., Inagaki Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   74 ( 3 )   248 - 249   2008.8

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  • (400) Genes Expressed in Tissue-cultured Seedlings of Mountain Laurel (Kalmia latifolia L.) with Colonizing Streptomyces padanus AOK30 (II)(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Meguro A., Ogiyama H., Hasegawa S., Nishimura T., Kunoh H., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   74 ( 3 )   273 - 274   2008.8

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  • (128) Genes Expressed in Leaves of Medicago truncatula in Response to Fungal Suppressor from Mycosphaerella pinodes(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Toyoda K., Kawanishi Y., Matsuzaki M., Inagaki Y., Ichinose Y., Shiraishi T.

    Annals of the Phytopathological Society of Japan   74 ( 3 )   200 - 201   2008.8

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  • (309) Plant Recognizes Bacterial DNA and Induces Defense Responses(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Yakushiji S., Inagaki Y., Toyoda K., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   74 ( 3 )   249 - 249   2008.8

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  • (3)Mycosphaerella pinodes Infection and Differences in Host Disease Resistance among Medicago truncatula Ecotypes(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

    Minakuchi Y., Maeda A., Inagaki Y., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   74 ( 1 )   56 - 56   2008.2

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  • (4)Heterologous Expressoin of Medicago truncatula Mtapy1;1 Gene in Nicotiana benthamiana and Characterization of Potential Interactor(s) for Cell Wall Apyrase(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

    Komatsu Y., Nagai H., Kawakami E., Inagaki Y., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   74 ( 1 )   57 - 57   2008.2

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  • (81)Genes Expressed in Tissue-cultured Seedlings of Mountain Laurel (Kalmia latifolia L.) with Colonizing Endophytic Streptomyces padanus AOK30(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

    Meguro A., Ogiyama H., Hasegawa S., Nishimura T., Kunoh H., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   74 ( 1 )   77 - 77   2008.2

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  • (71)Functional Analysis of Flagellar Motility and Elicitor Activity of Flagellar Protein Flagellin in Pseudomonas syringae pv. tabaci(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

    Naito K., Taguchi F., Inagaki Y., Toyoda K., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   74 ( 1 )   75 - 75   2008.2

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  • Modulation of defense signal transduction by flagellin-induced WRKY41 transcription factor in Arabidopsis thaliana.

    Higashi, K, Ishiga, Y, Inagaki, Y, Toyoda, K, Shiraishi, T, Ichinose, Y

    Mol. Genet. Genomics   279 ( 3 )   303 - 312   2008

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  • Molecular analysis for genome structure and gene expression of Oxidosqualene Cyclase genes in rice seedling

    Yoshishige Inagaki, Yukako Mutsukado, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose, Anne Osbourn

    GENES & GENETIC SYSTEMS   82 ( 6 )   521 - 521   2007.12

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  • (364) Activity of Bacterial DNA to Induce Plant Immunity(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Yakushiji S., Ishiga Y., Toyoda K., Inagaki Y., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   73 ( 3 )   272 - 272   2007.8

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  • (134) Rer1, a Novel Gene Induced during Establishment of Susceptibility in Nicotiana benthamiana(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Tanaka Y., Yoshihiro M., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   73 ( 3 )   210 - 210   2007.8

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  • (133) Heterologous Expression and Functional Analyses of Four Medicago truncatula Apyrase Genes(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Nagai H., Kawakami E., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   73 ( 3 )   210 - 210   2007.8

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  • (365) Effect of Iron Acquisition on the Swarming Motility in Pseudomonas syringae pv. tabaci(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Kawamoto K., Taguchi F., Toyoda K., Inagaki Y., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   73 ( 3 )   272 - 272   2007.8

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  • (132) Transcnptional Profiles of Medicago truncatula Cultured Cells Treated with a Fungal Suppressor from Mycosphaerella pinodes and Their Comparison with Those of the Differentiated Leaf Tissues(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Matsuzaki M., Matsuo K., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   73 ( 3 )   209 - 210   2007.8

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  • (361) Modulation of Defense Signal Transduction by Flagellin-induced WRKY41 Transcription Factor in Arabidopsis thaliana(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Higashi K., Ishiga Y., Toyoda K., Inagaki Y., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   73 ( 3 )   271 - 271   2007.8

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  • (362) Functional Analysis of Glycosyltransferases for Flagellin Glycosylation in Pseudomonas syringae(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Tsunemi K., Taguchi F., Yasuda C., Toyoda K., Inagaki Y., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   73 ( 3 )   271 - 272   2007.8

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  • (363) Response to Pathogen in Arabidopsis Variegated var2 Mutant(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Aono M., Miura E., Kato Y., Toyoda K., Inagaki Y., Shiraishi T., Sakamoto W., Ichinose Y.

    Annals of the Phytopathological Society of Japan   73 ( 3 )   272 - 272   2007.8

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  • (360) Function of Flagellar Motor Proteins and Virulence of Motor-Defective Mutants in Pseudomonas syringae pv. tabaci(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    Kenmotsu T., Taguchi F., Suzuki T., Toyoda K., Inagaki Y., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   73 ( 3 )   271 - 271   2007.8

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  • Analysis of Flagellum Motor-defective Mutants of Pseudomonas syringae pv. tabaci(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2006)

    Kenmotsu T., Taguchi F., Toyoda K., Shiraishi T., Ichonose Y.

    Annals of the Phytopathological Society of Japan   73 ( 1 )   72 - 72   2007.2

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  • Regulation of Defense Responses in Medicago truncatula Cells by Fungal Signal Molecules from Mycosphaerella pinodes(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2006)

    Matsuo K., Matsuzaki M., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   73 ( 1 )   54 - 54   2007.2

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  • Functional Analysis of the Flagellin-induced Gene Encoding WRKY41 Transcription Factor in Arabidopsis thaliana(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2006)

    Higashi K., Ishiga Y., Toyoda K., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   73 ( 1 )   72 - 72   2007.2

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  • N-terminal domain including conserved flg22 is required for flagellin-induced hypersensitive cell death in Arabidopsis thaliana.

    Naito, K, Ishiga, Y, Toyoda, K, Shiraishi, T, Ichinose, Y

    J. Gen. Plant Pathol.   73 ( 4 )   281 - 285   2007

  • Ultrastructural features of Mycosphaerella pinodes Infection and differenced in host susceptibility responses among Medicago truncatula Ecotypes.

    Suzuki, T, Maeda, A, Hirose, M, Ichinose, Y, Toyoda, K, Shiraishi, T

    J. Electr. Microsc. Technol. Med. Biol.   20 ( 2 )   167 - 168   2007

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  • (116) Plant Cell Wall Apyrase Recognizes and Responses to Diverse Pathogen-associated Molecular Patterns (PAMPs)(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

    Takahashi H., Hirakawa Y., Morishita K., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   72 ( 4 )   232 - 232   2006.11

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  • (118) Expression of Defense Related Genes in Medicago truncatula and Its Regulation by Fungal Signal Molecules from Mycosphaerella pinodes(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

    Matsuzaki M., Maeda A., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   72 ( 4 )   233 - 233   2006.11

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  • (117) Tolerance to Bacterial Infection in Transgenic Tobacco Plants Expressing PsAPY1(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

    Nagai H., Tsujimura K., Takahashi H., Kiba K., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   72 ( 4 )   232 - 233   2006.11

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  • (404) Recognition of Pseudomonas syringae pv. tabaci Flagellin in Arabidopsis thahana(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

    Naito K., Miyoshi R., Ishiga Y., Taguchi F., Toyoda K., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   72 ( 4 )   310 - 310   2006.11

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  • (431) Analysis of Virulence Factors and Screening of Swarming-specific Genes in Pseudomonas syringae pv. tabaci(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

    Asai S., Taguchi F., Toyoda K., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   72 ( 4 )   317 - 317   2006.11

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  • (119) Cytological Analyses of Mycosphaerella pinodes Infection and Differences in Host Response among Medicago truncatula Ecotypes(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

    Suzuki T., Maeda A., Hirose M., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   72 ( 4 )   233 - 233   2006.11

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  • (120) Phenotypic Characterization of Medicago truncatula Mutants with Decreased Susceptibility to Mycosphaerella pinodes Infection(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

    Maeda A., Suzuki T., Hirose M., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   72 ( 4 )   233 - 233   2006.11

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  • (430) Effect of Chemotaxis in Motility and Virulence of Pseudomonas syringae pv. tabaci(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

    Ogawa Y., Taguchi F., Toyoda K., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   72 ( 4 )   317 - 317   2006.11

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  • (121) Pea Cell-wall-redox Enzymes and Recognition/Response to Pathogens(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

    Kasai T., Osaki O., Suzuki T., Toyoda K., Kiba A., Inagaki Y., Ichinose Y., Shiraishi T.

    Annals of the Phytopathological Society of Japan   72 ( 4 )   234 - 234   2006.11

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  • Identification of glycosylation genes and glycosylated amino acids of flagellin in Pseudomonas syringae pv. tabaci

    F Taguchi, K Takeuchi, E Katoh, K Murata, T Suzuki, M Marutani, T Kawasaki, M Eguchi, S Katoh, H Kaku, C Yasuda, Y Inagaki, K Toyoda, T Shiraishi, Y Ichinose

    CELLULAR MICROBIOLOGY   8 ( 6 )   923 - 938   2006.6

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    A glycosylation island is a genetic region required for glycosylation. The glycosylation island of flagellin in Pseudomonas syringae pv. tabaci 6605 consists of three orfs: orf1, orf2 and orf3. Orf1 and orf2 encode putative glycosyltransferases, and their deletion mutants, Delta orf1 and Delta orf2, exhibit deficient flagellin glycosylation or produce partially glycosylated flagellin respectively. Digestion of glycosylated flagellin from wild-type bacteria and non-glycosylated flagellin from Delta orf1 mutant using aspartic N-peptidase and subsequent HPLC analysis revealed candidate glycosylated amino acids. By generation of site-directed Ser/Ala-substituted mutants, all glycosylated amino acid residues were identified at positions 143, 164, 176, 183, 193 and 201. Matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (MS) analysis revealed that each glycan was about 540 Da. While all glycosylation-defective mutants retained swimming ability, swarming ability was reduced in the Delta orf1, Delta orf2 and Ser/Ala-substituted mutants. All glycosylation mutants were also found to be impaired in the ability to adhere to a polystyrene surface and in the ability to cause disease in tobacco. Based on the predicted tertiary structure of flagellin, S176 and S183 are expected to be located on most external surface of the flagellum. Thus the effect of Ala-substitution of these serines is stronger than that of other serines. These results suggest that glycosylation of flagellin in P. syringae pv. tabaci 6605 is required for bacterial virulence. It is also possible that glycosylation of flagellin may mask elicitor function of flagellin molecule.

    DOI: 10.1111/j.1462-5822.2005.0674.x

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  • Functional analysis of Gac two-component system in P syringae pv. tabaci

    M. Marutani, Y. Ogawa, F. Taguchi, Y. Inagaki, K. Toyoda, T. Shiraishi, Y. Ichinose

    PHYTOPATHOLOGY   96 ( 6 )   S74 - S74   2006.6

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  • Ultrastructural Features of Mycosphaerella pinodes Infection and Differences in Host Susceptibility Responses among of Medicago truncatula (Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

    Suzuki T., Maeda A., Hirose M., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   72 ( 1 )   65 - 66   2006.2

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  • Functional Analysis of AtOPR1 and 2 in the Interactions with Fungal Pathogens using AtOPR1-and AtOPR2-Overexpressed Transgenic Arabidopsis (2) (Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

    Matsui H., Itazaki M., Toyoda K., Inagaki Y., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   72 ( 1 )   66 - 66   2006.2

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  • Analysis of AHL-production Defective Mutant, Δorf3 of Pseudomonas syringae pv. tabaci (Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

    Taguchi F., Ogawa Y., Suzuki T., Takeuchi K., Toyoda K., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   72 ( 1 )   79 - 79   2006.2

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  • HR-inducible Domain of Pseudomonas syringae pv. tabaci Flagellin in Arabidopsis thaliana (Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

    Naito K., Ishiga Y., Taguchi F., Takeuchi K., Katoh E., Toyoda K., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   72 ( 1 )   78 - 79   2006.2

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  • Signal Transduction through the Plant Cell Wall-plasma Membrane Continuum (Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

    Hirai Y., Toyoda K., Ichinose Y., Shiraishi T.

    Annals of the Phytopathological Society of Japan   72 ( 1 )   66 - 66   2006.2

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  • A pea NTPase, PsAPY1, recognizes signal molecules from microorganisms

    KIBA Akinori, TOYODA Kazuhiro, YOSHIOKA Kazuaki, TSUJIMURA Kami, TAKAHASHI Hirotaka, ICHINOSE Yuki, TAKEDA Tadahiro, KATO Toshiaki, SHIRAISHI Tomonori

    J Gen Plant Pathol   72 ( 4 )   238 - 246   2006

  • Peas extracellular Cu/Zn-superoxide dismutase responsive to signal molecules from a fungal pathogen.

    Kasai, T, Suzuki, T, Ono, K, Ogawa, K, Inagaki, Y, Ichinose, Y, Toyoda, K, Shiraishi, T

    J. Gen. Plant Pathol.   72 ( 5 )   265 - 272   2006

  • A homologue of the 3-oxoacyl-(acyl carrier protein) synthase III gene located in the glycosylation island of Pseudomonas syringae pv. tabaci regulates virulence factors via N-acyl homoserine lactone and fatty acid synthesis

    Taguchi Fumiko, Ogawa Yujiro, Takeuchi Kasumi, Suzuki Tomoko, Toyoda Kazuhiro, Shiraishi Tomonori, Ichinose Yuki

    Journal of Bacteriology   188 ( 24 )   8376 - 8384   2006

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    DOI: 10.1128/JB.00763-06

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  • A binding protein for fungal signal molecules in cell wall of Pisum sativum.

    Kiba, A, Ohgawara, T, Toyoda, K, Inoue-Ozaki, M, Takeda, T, Rao, U.S, Kato, T, Ichinose, Y, Shiraishi, T

    J. Gen. Plant Pathol.   72 ( 4 )   228 - 237   2006

  • Induction of defense responses in pea tissues by inorganic phosphate.

    Kawahara, T, Namba, H, Toyoda, K, Kasai, T, Sugimoto, M, Inagaki, Y, Ichinose, Y, Shiraishi, T

    J. Gen. Plant Pathol.   72 ( 3 )   129 - 136   2006

  • AtOPR3高発現シロイヌナズナを用いた植物病原菌相互作用におけるOPR subgroup 2の機能解析

    松井英譲, 豊田和弘, 稲垣善茂, 白石友紀, 一瀬勇規

    日本植物病理学会報   72 ( 4 )   2006

  • (12) Esterase Activity of Extracellular Material Released from Powdery Mildew Conidia on Artificial Membrane and Plant Surfaces(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Suzuki T., Fujita K., Carver T. L., Thomas B., Toyoda K., Shiraishi T., Kunoh H.

    Annals of the Phytopathological Society of Japan   71 ( 3 )   188 - 188   2005.8

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  • (18) Rer1 Gene Homologues Expressed in Medicago truncatula and Barley Challenged with Virulent Pathogens(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Yoshihiro M., Hirose M., Fujita K., Inagaki Y., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   71 ( 3 )   190 - 190   2005.8

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  • (10) Silencing of NbCdc27B Induces Defense-Related Gene Expression and Resistance to C. lagenarium(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Kudo C., Inagaki Y. S., Fukuoka S., Suzuki T., Takano Y., Okuno T., Asai S., Suenaga H., Sasabe M., Toyoda K., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   71 ( 3 )   187 - 188   2005.8

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  • (17) Medicago truncatula Genes Expressed in Susceptible Interaction with Mycosphaerella pinodes; Potential Role of Cellulose Synthase Gene(s) in Achievement of Susceptibility to Fungal Pathogen(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Kohya N., Hirose M., Hata T., Inagaki Y., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   71 ( 3 )   189 - 190   2005.8

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  • (33) Differential Expression of Strawberry Genes Induced by Host-Specific AF-toxins Produced by the Strawberry Pathotype of Alternaria alternata (3) : Specific Gene Expression Induced by AF-toxin I and II(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Kitani K., Toyoda K., Shiraishi T., Yamamoto M.

    Annals of the Phytopathological Society of Japan   71 ( 3 )   194 - 194   2005.8

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  • (19) Functional Analysis of AtOPR1 and 2 in the Interactions with Fungal Pathogens using AtOPR1- and AtOPR2-Overexpressed Transgenic Arabidopsis(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Matsui H., Itazaki M., Toyoda K., Inagaki Y., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   71 ( 3 )   190 - 190   2005.8

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  • (13) Pea Apoplastic Cu/Zn-SOD Responds to Pathogen Signal Molecules(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Kasai T., Suzuki T., Toyoda K., Inagaki Y., Ichinose Y., Shiraishi T.

    Annals of the Phytopathological Society of Japan   71 ( 3 )   188 - 188   2005.8

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  • (14) Plant Cell Wall as the First Line of Defense Involving Perception of Pathogen Signals and the Subsequent Transcriptional Response in Pea-Mycosphaerelas pinodes Interaction(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Hirai Y., Inagaki Y., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   71 ( 3 )   188 - 189   2005.8

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  • (16) Molecular and Genetic Dissection of Plant Susceptibility to Fungal Pathogen; Toward Isolation of Plant Susceptibility Gene(s) Required for the Achievement of Successful Infection and the Subsequent Pathogen Growth(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Hirose M., Maeda A., Iwahashi K., Ikeda S., Kohya N., Yoshihiro M., Matusuo K., Nagai C., Kawakami E., Hata T., Tanaka Y., Inagaki Y., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   71 ( 3 )   189 - 189   2005.8

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  • (11) Isolation of Insensitive Mutants to INF1 Elicitin and Expression Pattern of Defense Genes in the A04 Mutant(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Asai S., Inagaki Y. S., Kudo C., Ishikawa A., Toyoda K., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   71 ( 3 )   188 - 188   2005.8

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  • (15) Modification of Plant Virulence Target Alters the Resistance and Susceptibility to Pathogens(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Tsujimura K., Kawahara T., Yoshioka K., Nagai C., Kiba K., Inagaki Y., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   71 ( 3 )   189 - 189   2005.8

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  • (406) Orf3 Gene in the Glycosylation Island of Pseudomonas syringae pv. tabaci Regulates Expression of Virulence-Related Genes Via Quorum Sensing Molecules(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Ogawa Y., Taguchi F., Marutani M., Inagaki Y., Toyoda K., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   71 ( 3 )   294 - 294   2005.8

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  • (430) Defense Responses of Arabidopsis thaliana Treated with PAMPs from Pseudomonas syringae(Abstracts of the Paper Presented at the 2005 Annual Meeting in Shizuoka)

    Ishiga Y., Suenaga H., Inagaki Y., Toyoda K., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   71 ( 3 )   300 - 300   2005.8

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  • (405) Functional Analysis of Gac Two-Component System in Pseudomonas syringae pv. tabaci(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

    Marutani M., Ogawa Y., Taguchi F., Toyoda K., Inagaki Y., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   71 ( 3 )   294 - 294   2005.8

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  • (53) Protein Expression and Enzyme Activity of AFT8, a Gene Found in the AF-toxin Biosynthesis Gene Cluster of the Alternaria alternata Strawberry Pathotype(Abstracts Presented at the Meeting of the Kansai Division, Matsuyama, October 9-10, 2004, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

    Kitani K., Ruswandi S., Takahara H., Hosaki Y., Akimitsu K., Tsuge T., Toyoda K., Shiraishi T., Yamamoto M.

    Annals of the Phytopathological Society of Japan   71 ( 1 )   66 - 66   2005.2

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  • (4) A Medicago truncatula-Mycosphaerella pinodes Interaction, a New Model Pathosystem for Genetic and Molecular Dissection of Susceptibility to Fungal Pathogen. VI : A M. truncatula Suspension Cultured Cell Line with Responsiveness to Pathogen Signal Molecules and in vitro Regeneration(Abstracts Presented at the Meeting of the Kansai Division, Matsuyama, October 9-10, 2004, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

    Matsuo K., Tani C., Inagaki Y., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   71 ( 1 )   53 - 53   2005.2

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  • (3) A Medicago truncatula-Mycosphaerella pinodes Interaction, a New Model Pathosystem for Genetic and Molecular Dissection of Susceptibility to Fungal Pathogen V. : Genes Expressed in M. truncatula Leaves during the Establishment of a Compatible Interaction with a Foliar Pathogen, M. pinodes(Abstracts Presented at the Meeting of the Kansai Division, Matsuyama, October 9-10, 2004, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

    Hirose M., Yoshihiro M., Hata T., Inagaki Y., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   71 ( 1 )   52 - 53   2005.2

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  • (1) Establishment of Gene Disruption Experiment in Mycosphaerella pinodes(Abstracts Presented at the Meeting of the Kansai Division, Matsuyama, October 9-10, 2004, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

    Takahara H., Miyake Y., Inagaki Y., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   71 ( 1 )   52 - 52   2005.2

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  • (42) Does APC Involve in INF1 Elicitin-Induced Hypersensitive Cell Death?(Abstracts Presented at the Meeting of the Kansai Division, Matsuyama, October 9-10, 2004, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

    Kudo C., Fukuoka S., Suenaga H., Sasabe M., Asai S., Toyoda K., Inagaki Y. S., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   71 ( 1 )   63 - 63   2005.2

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  • (43) Isolation of Insensitive Mutants to INF1 Elicitin in Nicotiana benthamiana(Abstracts Presented at the Meeting of the Kansai Division, Matsuyama, October 9-10, 2004, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

    Asai S., Ishikawa A., Kudo C., Toyoda K., Inagaki Y. S., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   71 ( 1 )   63 - 63   2005.2

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  • (57) Defense Responses of Arabidopsis thaliana Treated with Flagellin from Pseudomonas syringae pv. tabaci Wild Type and Oligopeptide flg22 (2)(Abstracts Presented at the Meeting of the Kansai Division, Matsuyama, October 9-10, 2004, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

    Ishiga Y., Lin Y., Taguchi F., Inagaki Y., Toyoda K., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   71 ( 1 )   67 - 67   2005.2

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  • (2) Response to Fungal Pathogen in Transgenic Tobacco Plants Overexpressing Pea Apyrase Gene (PsAPY1)(Abstracts Presented at the Meeting of the Kansai Division, Matsuyama, October 9-10, 2004, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

    Tsujimura K., Kiba A., Inagaki Y., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   71 ( 1 )   52 - 52   2005.2

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  • (5) A Medicago truncatula-Mycosphaerella pinodes Interaction, a New Model Pathosystem for Genetic and Molecular Dissection of Susceptibility to Fungal pathogen VII : Production of M. truncatula Mutant Library and Screening of Mutants with Decreased Susceptibility(Abstracts Presented at the Meeting of the Kansai Division, Matsuyama, October 9-10, 2004, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

    Toyoda K., Hirose M., Maeda A., Koya N., Matsuo K., Yoshihiro M., Tanaka Y., Kawakami E., Nagai C., Inagaki Y., Ichinose Y., Shiraishi T.

    Annals of the Phytopathological Society of Japan   71 ( 1 )   53 - 53   2005.2

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  • Pea apoplastic Cu/Zn-SOD responsive to pathogenic signal molecules

    T Kasai, K Ono, T Suzuki, K Toyoda, Y Inagaki, Y Ichinose, T Shiraishi

    PLANT AND CELL PHYSIOLOGY   46   S94 - S94   2005

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  • Defense responses of Arabidopsis thaliana inoculated with Pseudomonas syringae pv. tabaci wild type and defective mutants for flagellin (ΔfliC) and flagellin-glycosylation (Δorf1)

    ISHIGA Y

    J Gen Plant Pathol   71 ( 4 )   302 - 307   2005

  • Flagellin from Pseudomonas syringae pv. tabaci induced hrp-independent HR in tomato

    MARUTANI M

    J Gen Plant Pathol   71 ( 4 )   289 - 295   2005

  • Mechanism of flagellin-induced hypersensitive cell death in Arabidopsis thaliana

    Y Ishiga, YC Lin, M Marutani, F Taguchi, Y Inagaki, K Toyoda, T Shiraishi, Y Ichinose

    PLANT AND CELL PHYSIOLOGY   46   S94 - S94   2005

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  • (370) Defense Responses of Arabidopsis thaliana Treated with Flagellin from Pseudomonas syringae pv. tabaci Wild Type and Oligopeptide flg22(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

    Ishiga Y., Lin Y., Taguchi F., Inagaki Y., Toyoda K., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   70 ( 3 )   290 - 290   2004.8

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  • (45) Response of Cowpea Apyrase Genes to Fungal Elicitor or Suppressor from M. pinodes(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

    Takahashi H., Toyoda K., Hirakawa Y., Morishita K., Inagaki Y., Yamamoto M., Ichinose Y., Shiraishi T.

    Annals of the Phytopathological Society of Japan   70 ( 3 )   202 - 202   2004.8

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  • (46) A Medicago truncatula-Mycosphaerella pinodes Interaction, a New Model Pathosystem for Genetic Dissection of Susceptibility to Fungal Pathogen IV. : Further Efforts to Set up the Model Pathosystem for a Better Understanding of Fungal Suppressor-mediated Diseases(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

    Kawakami E., Kohya N., Hirose M., Nagai C., Matsuo K., Tani C., Hata T., Morikawa J., Inagaki Y., Yamamoto M., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   70 ( 3 )   202 - 202   2004.8

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  • (64) Apyrase Is Not Required for INF1- and Avr9-induced Cell Death in Nicotiana benthamiana(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

    Nagai C., Yoshioka H., Inagaki Y., Yamamoto M., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   70 ( 3 )   207 - 207   2004.8

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  • (74) Differential Expression of Strawberry Genes Induced by Host-specific AF-toxins Produced by the Strawberry Pathotype of Alternaria alternata : (2) Quantitative Time Course of Differentially Expressed Genes(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

    Kitani K., Toyoda K., Shiraishi T., Yamamoto M.

    Annals of the Phytopathological Society of Japan   70 ( 3 )   210 - 210   2004.8

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  • (44) Characterization of the Genes Expressed during Spore Germination of Mycosphaerella pinodes(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

    Takahara H., Toyoda K., Tsuji G., Yamamoto M., Inagaki Y., Ichinose Y., Kubo Y., Shiraishi T.

    Annals of the Phytopathological Society of Japan   70 ( 3 )   201 - 202   2004.8

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  • (12) Establishment of Transformation System for Mycosphaerella pinodes to Isolate Virulence-Related Genes (Abstracts Presented at the Meeting of the Kansai Division) (Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

    Miyake Y., Takahara H., Toyoda K., Inagaki Y., Yamamoto M., Ichinose Y., Shiraishi T.

    Annals of the Phytopathological Society of Japan   70 ( 1 )   59 - 59   2004.2

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  • (11) A Medicago truncatula-Mycosphaerella pinodes Interaction, a New Model Pathosystem for Genetic Dissection of Susceptibility to Fungal Pathogen III. : Establishment of Bioassay System (Abstracts Presented at the Meeting of the Kansai Division) (Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

    Morikawa J., Ikeda S., Inagaki Y., Yamamoto M., Ichinose Y., Toyoda K., Shiraishi T.

    Annals of the Phytopathological Society of Japan   70 ( 1 )   58 - 59   2004.2

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  • Differential regulation of MBP kinases by a glycoproptein elicitor and a polypeptide suppressor from Mycosphaerella pinodes in pea

    Uppalapati, SR, K Toyoda, Yasuhiro, I, Y Ichinose, T Shiraishi

    PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY   64 ( 1 )   17 - 25   2004.1

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    A polypeptide fungal suppressor from a pea pathogen Mycosphaerella pinodes plays a key role in pathogenesis by suppressing elicitor-induced defense response(s) in pea (Pisum sativum L). In this study, we show that treatment of pea tissues with the polysaccharide elicitor secreted by M. pinodes results in rapid increased activation of two myelin basic protein (MBP)-dependent kinases p44 (approximate to44 kDa) and p48 (approximate to48 kDa) within 15-30 min upon elicitation. Interestingly, the suppressor inhibited the elicitor-induced activation of only p44 kinase. While the defense-inducing signalling molecules, chitosan and salicylic acid (SA) activated the p44 and p48 kinases, methyl jasmonate (MeJA) did not. The abiotic stress signals, abscisic acid (ABA), NaCl and wounding activated the p48 kinase alone. These results demonstrate that MAPKs are differentially activated in response to pathogen invasion and abiotic stress in pea. Furthermore, specific inhibition of elicitor-induced p44 kinase activation by a MAPKK inhibitor, PD098059 and protein kinase inhibitor, K252a correlated with the suppression of elicitor-induced phenylalanine ammonia lyase (PAL) gene expression, supporting a role for p44 in the elicitor-induced defense response(s) in pea. Inhibition of p44 by the phosphoinositide (PI) turnover inhibitor, neomycin (a fungal suppressor mimic), and potentiation of p44 by the diacylglycerol (DAG) kinase inhibitor, R59022 indicated that p44 may be acting downstream of (PI) metabolism. Taken together, our results indicate that suppressor of defense elicitation from M. pinodes acts through inhibition of a MAPK (p44), possibly through a PI signaling pathway, facilitating the establishment of basic compatibility during infection of pea. (C) 2004 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.pmpp.2004.05.003

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  • PDR-type ABC transporter and tobacco defense response against pathogen

    Y Ichinose, D Schenke, M Sasabe, Y Inagaki, K Toyoda, T Shiraishi

    PLANT AND CELL PHYSIOLOGY   45   S1 - S1   2004

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  • Resistance in Pea Plants induced by Carpropamid and Related Compounds(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

    Matsumoto M., Akisada S., Araki Y., Sawada H., Toyoda K., Yamamoto M., Inagaki Y., Ichinose Y., Shiraishi T.

    Annals of the Phytopathological Society of Japan   69 ( 3 )   255 - 256   2003.8

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  • A Medicago truncatula-Mycosphaerella pinodes Interaction, a New Model for Genetic Dissection of Susceptibility to Fungal Pathogen II. Achievement of Susceptibilty by Fungal Suppresicins(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

    Ikeda S., Morikawa J., Toyoda K.

    Annals of the Phytopathological Society of Japan   69 ( 3 )   247 - 248   2003.8

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  • The Role of Diamineoxidase in Plant Defense 2. Regulatory Mechanism on Its Activation(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

    Iwahashi K., Ohwatari Y., Kawahara T., Toyoda K., Yamamoto M., Inagaki Y., Ichinose Y., Shiraishi T.

    Annals of the Phytopathological Society of Japan   69 ( 3 )   250 - 250   2003.8

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  • Analysis of Pea Apoplastic Cu/Zn-SOD Responsive to Pathogenic Signal Molecules(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

    Kasai T., Ono K., Toyoda K., Inagaki Y., Yamamoto M., Ichinose Y., Shiraishi T.

    Annals of the Phytopathological Society of Japan   69 ( 3 )   250 - 250   2003.8

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  • Characterization of Plasma Membrane Proteins Affinity-purified with Concanavalin A (Con A), a Potent Elicitor of Phytoalexin Accumulation(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

    Okazaki A., Toyoda K., Sugimoto M., Inagaki Y., Yamamoto M., Ichinose Y., Shiraichi T.

    Annals of the Phytopathological Society of Japan   69 ( 3 )   249 - 250   2003.8

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  • Construction of subracted cDNA Library of Strawberry Treated by AF-toxins from Alternaria alternata Strwberry Pathotype(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

    Kitani K., Toyoda K., Shiraishi T., Yamamoto M.

    Annals of the Phytopathological Society of Japan   69 ( 3 )   252 - 253   2003.8

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  • Flagellin-induced HR Cell Death Is Independent of Type III Secretion System in Pseudomonas syringae pv. tabaci(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

    Marutani M., Taguchi F., Toyoda K., Inagaki Y., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   69 ( 3 )   300 - 301   2003.8

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  • Medicago truncatula-Mycosphaerella pinodes Interaction, a New Model for the Molecular and Genetic Dissection of Plant-microbe Interaction

    Toyoda K., Ikeda S., Morikawa J., Inagaki Y., Ichinose Y., Yamamoto M., Shiraishi T.

    Annals of the Phytopathological Society of Japan   69 ( 1 )   56 - 56   2003.2

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  • Endogenous Suppressor in Arabidopsis thaliana

    Rao U.S., Matsushita H., Toyoda K., Ichinose Y., Inagaki Y., Yamamoto M., Tsuji G., Kubo Y., Shiraishi T.

    Annals of the Phytopathological Society of Japan   69 ( 1 )   56 - 56   2003.2

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  • Extracellular PO_4^- induces the expression of peroxidases in Pisum sativum

    Kawahara T., Nanba H., Toyoda K., Inagaki Y., Ichinose Y., Yamamoto M., Shiraishi T.

    Annals of the Phytopathological Society of Japan   69 ( 1 )   56 - 57   2003.2

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  • Characterization of elicitor-responsive receptor-like protein kinase in tobacco

    Sasabe M., Toyoda K., Inagaki Y., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   69 ( 1 )   56 - 56   2003.2

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  • Analysis of Suppressor-responsive Genes : (5) Expression of OPR Gene Family

    Matsui H., Nakamura G., Ishiga Y., Toyoda K., Inagaki Y., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   69 ( 1 )   57 - 57   2003.2

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  • Possible Involvement of AAAG Motif and PsDof1 in Elicitor-Induced Gene Expression in Pea

    Seki Hikaru, Marutani Mizuri, Inagaki Yoshishige, Toyoda Kazuhiro, Shiraishi Tomonori, Ichinose Yuki

    92 ( 1 )   21 - 26   2003.2

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  • Need for flagella for complete virulence of Pseudomonas syringae pv. tabaci : genetic analysis with flagella-defective mutants ΔfliC and ΔfliD in host tobacco plants

    ICHINOSE Yuki, SHIMIZU Rena, IKEDA Yoko, TAGUCHI Fumiko, MARUTANI Mizuri, MUKAIHARA Takafumi, INAGAKI Yoshishige, TOYODA Kazuhiro, SHIRAISHI Tomonori

    J. Gen. Plant Pathol.   69 ( 4 )   244 - 249   2003

  • Expression of allene oxide synthase and allene oxide cyclase in the interactions between pea and fungal pathogens

    ISHIGA Yasuhiro, INAGAKI Yoshishige, TOYODA Kazuhiro, SHIRAISHI Tomonori, ICHINOSE Yuki

    J Gen Plant Pathol   69 ( 6 )   351 - 357   2003

  • Phylogenetic Classification of Dof- type Transcription Factors in Pea (Pisum sativum)

    NAKAMURA Naoki, MARUTANI Mizuri, SANEMATSU Shiroh, TOYODA Kazuhiro, INAGAKI Yoshi-shige, SHIRAISHI Tomonori, ICHINOSE Yuki

    Plant tissue culture letters   20 ( 3 )   247 - 252   2003

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    The Dof protein family is a group of plant transcription factors carrying highly conserved 52 residues referred to as the Dof domain, and belongs to the C4 zinc-finger transcription factors. We isolated various PsDof genes by PCR using the Dof-domain nucleotide sequence of the PsDof1 gene from a cDNA library of elicitor-treated pea epicotyls, and these isolated PsDof genes were then classified phylogenetically. Since the obtained genes (PsDof1 to PsDof7) were scattered over various positions of the phylogenetic tree, they were expected to perform various functions as Dof-type transcription factors. From their positions in the tree, it is expected that the PsDof2 and PsDof5 genes are defense related, as is the PsDof1 gene.

    DOI: 10.5511/plantbiotechnology.20.247

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  • Flagella-mediated Interactions between Phytopathogenic Bacteria and Plants

    ICHINOSE Yuki, TAGUCHI Fumiko, INAGAKI Yoshishige, TOYODA Kazuhiro, SHIRAISHI Tomonori

    41 ( 8 )   511 - 516   2003

  • Post-translational modification of flagellin determines the specificity of HR induction

    TAGUCHI F

    Plant Cell Physiol   44 ( 3 )   342 - 349   2003

  • Cloning and characterization of pea apyrases: Involvent of psAPY1 in response to signal molecules from the pea pathogen, Mycosphaerella pinodes

    Journal of plant pathology   69, 33-38   2003

  • Differential effects of flagellins from Pseudomonas syringae pv. tabaci, tomato and glycinea on plant defense response

    TAGUCHI F

    Plant Physiol Biochem   41   165 - 174   2003

  • Role of flagella and flagellin in plant - Pseudomonas syringae interactions

    Y Ichinose, R Shimizu, F Taguchi, K Takeuchi, M Marutani, T Mukaihara, Y Inagaki, K Toyoda, T Shiraishi

    PSEUDOMONAS SYRINGAE AND RELATED PATHOGENS: BIOLOGY AND GENETICS   pp. 311-318.   311 - 318   2003

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    The role of flagella and monomer flagellin of Pseudomonas syringae pv. tabaci in plant-bacteria interactions was investigated by using non-polar fliC or fliD mutants. These mutants deleted the open reading frames for fliC and fliD, respectively, and both mutants lost all flagella and motility. The DeltafliC mutant did not produce flagellin, whereas DeltafliD mutant, that lost HAP2 protein, secreted a large amount of monomer flagellin in the culture medium. Inoculation of tomato leaves with wild type and DeltafliD mutant of P. s. pv. tabaci induced HR, whereas the DeltafliC mutant caused symptom-like change and propagated as P. s. pv. tomato. In tomato suspension cultured cells, wild type P. s. pv. tabaci induced visible HR-like changes. The DeltafliC mutant did not induce the HR, but the response was activated by the DeltafliD mutant. The expression of typical defence genes such as PAL and hsr203J was rapidly and strongly induced by inoculation with the DeltafliD mutant compared to inoculation with wild type P. s. pv. tabaci. On the other hand, both fliC and fliD mutants were reduced in virulence when inoculated into host tobacco leaves. Furthermore, complementation of fliC gene in DeltafliC mutant restored motility and HR-inducing ability in tomato, and virulence in tobacco. These results suggest that the monomer flagellin of P. s. pv. tabaci is an essential factor in the elicitation of HR in non host tomato cells, and flagella are required for complete virulence in host tobacco cells.

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  • Resistance and susceptibility of plants to fungal pathogens

    K Toyoda, NC Collins, A Takahashi, K Shirasu

    TRANSGENIC RESEARCH   11 ( 6 )   567 - 582   2002.12

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    Plants are under continuous threat of infection by pathogens endowed with diverse strategies to colonize their host. Comprehensive biochemical and genetic approaches are now starting to reveal the complex signaling pathways that mediate plant disease resistance. Initiation of defense signaling often involves specific recognition of invading pathogens by the products of specialized host resistance (R) genes. Potential resistance signaling components have been identified by mutational analyses to be required for specific resistance in the model Arabidopsis and some crop species. Strikingly, many of the components share similarity to that of innate immune systems in animals. Evidence is also accumulating that plant pathogens have a number of ways to evade host defenses during the early stages of infection, similar to animal pathogens. These strategies are becoming much better understood in a number of plant- pathogen interactions. In this review, we focus on the current knowledge of host factors that control plant resistance and susceptibility to fungal pathogens. The knowledge accumulated in these studies will serve a fundamental basis for combating diseases in strategic molecular agriculture.

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  • Expression of the 12-oxophytodienoic acid 10,11-reductase gene in the compatible interaction between pea and fungal pathogen

    Y Ishiga, A Funato, T Tachiki, K Toyoda, T Shiraishi, T Yamada, Y Ichinose

    PLANT AND CELL PHYSIOLOGY   43 ( 10 )   1210 - 1220   2002.10

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    Suppressors produced by Mycosphaerella pinodes are glycopeptides to block pea defense responses induced by elicitors. A clone, S64, was isolated as cDNA for suppressor-inducible gene from pea epicotyls. The treatment of pea epicotyls with suppressor alone induced an increase of S64 mRNA within 1 h, and it reached a maximum level at 3 h after treatment. The induction was not affected by application of the elicitor, indicating that the suppressor has a dominant action to regulate S64 gene expression. S64 was also induced by inoculation with a virulent pathogen, M. pinodes, but not by inoculation with a non-pathogen, Ascochyta rabiei, nor by treatment with fungal elicitor. The deduced structure of S64 showed high homology to 12-oxophytodienoic acid reductase (OPR) in Arabidopsis thaliana. A recombinant protein derived from S64 had OPR activity, suggesting compatibility-specific activation of the octadecanoid pathway in plants. Treatment with jasmonic acid (JA) or methyl jasmonic acid, end products of the octadecanoid pathway, inhibited the elicitor-induced accumulation of PAL mRNA in pea. These results indicate that the suppressor-induced S64 gene expression leads to the production of JA or related compounds, which might contribute to the establishment of compatibility by inhibiting the phenylpropanoid biosynthetic pathway.

    DOI: 10.1093/pcp/pcf144

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  • Expression of the 12-oxophytodienoic acid 10,11-reductase gene in the compatible interaction between pea and fungal pathogen

    Y Ishiga, A Funato, T Tachiki, K Toyoda, T Shiraishi, T Yamada, Y Ichinose

    PLANT AND CELL PHYSIOLOGY   43 ( 10 )   1210 - 1220   2002.10

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    Suppressors produced by Mycosphaerella pinodes are glycopeptides to block pea defense responses induced by elicitors. A clone, S64, was isolated as cDNA for suppressor-inducible gene from pea epicotyls. The treatment of pea epicotyls with suppressor alone induced an increase of S64 mRNA within 1 h, and it reached a maximum level at 3 h after treatment. The induction was not affected by application of the elicitor, indicating that the suppressor has a dominant action to regulate S64 gene expression. S64 was also induced by inoculation with a virulent pathogen, M. pinodes, but not by inoculation with a non-pathogen, Ascochyta rabiei, nor by treatment with fungal elicitor. The deduced structure of S64 showed high homology to 12-oxophytodienoic acid reductase (OPR) in Arabidopsis thaliana. A recombinant protein derived from S64 had OPR activity, suggesting compatibility-specific activation of the octadecanoid pathway in plants. Treatment with jasmonic acid (JA) or methyl jasmonic acid, end products of the octadecanoid pathway, inhibited the elicitor-induced accumulation of PAL mRNA in pea. These results indicate that the suppressor-induced S64 gene expression leads to the production of JA or related compounds, which might contribute to the establishment of compatibility by inhibiting the phenylpropanoid biosynthetic pathway.

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  • (44)Plant Recognition and Response to Pathogens-Response of Non-host Plant's NTPase to Pathogen Signals

    Takahashi H., Hirakawa Y., Morishita K., Kawahara T., Yoshioka K., Toyoda K., Inagaki Y., Yamamoto M., Ichinose Y., Shiraishi T.

    Annals of the Phytopathological Society of Japan   68 ( 2 )   168 - 168   2002.8

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  • (43)Response of Cu/Zn-SOD in Pea Apoplast to Pathogen Signals

    Kasai T., Ono K., Toyoda K., Inagaki Y., Yamamoto M., Ichinose Y., Shiraishi T.

    Annals of the Phytopathological Society of Japan   68 ( 2 )   167 - 168   2002.8

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  • Hypersensitive Response-Inducing Factors Produced by Pseudomonas syringae (4) Functional Analysis of Flagellin Using Flagella-Deficient Mutants(Abstracts Presented at the Meeting of the Kansai Division)

    Shimizu R, Taguchi F, Ikeda Y, Toyoda K, Shiraishi T, Inagaki Y, Ichinose Y

    Annals of the Phytopathological Society of Japan   68 ( 1 )   100 - 100   2002.4

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  • Analysis of Elicitin-Responsive Genes in Tobacco BY-2 Cells, ATP-Binding Cassette Transporter Gene is Induced by INF1 Elicitin(Abstracts Presented at the Meeting of the Kansai Division)

    Sasabe M., Toyoda K., Shiraishi T., Inagaki Y., Ichinose Y.

    Annals of the Phytopathological Society of Japan   68 ( 1 )   92 - 93   2002.4

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  • Pseudomonas syringae pv. tabaciの単量体Flagellinは,非宿主植物トマト細胞にHR誘導因子として働く

    清水鈴菜, 田口富美子, 池田陽子, 豊田和弘, 白石友紀, 稲垣善茂, 一瀬勇規

    日本植物生理学会年会要旨集   42nd   238   2002.3

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  • 高等植物における病害抵抗性シグナル伝達と病原菌による制御

    日本植物病理学会、植物微生物相互作用研究の現状と将来展望   67 - 76   2002

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  • Cloning and characterization of pea apyrases: Involvent of psAPY1 in response to signal molecules from the pea pathogen, Mycosphaerella pinodes

    Journal of plant pathology   69, 33-38   2002

  • Cell wall-plasma membrane continuum is involved in transduction of pathogen signals

    K Toyoda, AM Kiba, T Kawahara, M Sugimoto, RS Uppalapati, Y Inagaki, Y Ichinose, M Yamamoto, T Shiraishi

    PLANT AND CELL PHYSIOLOGY   43   S10 - S10   2002

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  • Flagellin, a constituent of a flagella filament in Pseudomonas syringae pv. tabaci, is an intense HR elicitor as a monomer molecule on nonhost tomato cells

    R Shimizu, F Taguchi, Y Ikeda, K Toyoda, T Shiraishi, Y Inagaki, Y Ichinose

    PLANT AND CELL PHYSIOLOGY   43   S201 - S201   2002

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  • Gramine increase associated with rapid and transient systemic resistance in barley seedlings induced by mechanical and biological stresses

    H Matsuo, K Taniguchi, T Hiramoto, T Yamada, Y Ichinose, K Toyoda, K Takeda, T Shiraishi

    PLANT AND CELL PHYSIOLOGY   42 ( 10 )   1103 - 1111   2001.10

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    Systemic acquired resistance (SAR) is one of the intriguing issues for studying the mechanism in signal transduction system in a whole plant. We found that SAR and increase of an antifungal compound were induced rapidly and transiently in barley (Hordeum vulgare L. cv. Goseshikoku) by mechanical and biological stresses. One of the major antifungal compounds was identified as an indole alkaloid, gramine (N,N-dimethyl-3-aminomethylindole), by mass spectrum and NMR analyses. Gramine is well known as a constitutive compound of barley, but it increased significantly in the primary and secondary leaves of barley seedlings within 12 h after pruning or inoculating with the powdery mildew fungi of barley (Blumeria graminis Esp. hordei) and wheat (B. graminis Esp. tritici). However, in the leaf detached from unwounded seedlings or in the leaf inoculated with the barley powdery mildew fungus, gramine did not increase at all. In the water droplets contacted with barley leaves, the amount of leaked gramine increased dependently upon the time after the seedling was injured mechanically. We also found a tight correlation between gramine increase and enhancement of resistance to the barley powdery mildew fungus in barley leaves treated with an endogenous elicitor. Furthermore, such a systemic resistance was not observed in a barley cultivar Morex that lacks the biosynthetic pathway of gramine. From these results, we conclude that gramine is the excellent marker in rapid and transient systemic acquired resistance in barley.

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  • Gramine increase associated with rapid and transient systemic resistance in barley seedlings induced by mechanical and biological stresses

    H Matsuo, K Taniguchi, T Hiramoto, T Yamada, Y Ichinose, K Toyoda, K Takeda, T Shiraishi

    PLANT AND CELL PHYSIOLOGY   42 ( 10 )   1103 - 1111   2001.10

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    Systemic acquired resistance (SAR) is one of the intriguing issues for studying the mechanism in signal transduction system in a whole plant. We found that SAR and increase of an antifungal compound were induced rapidly and transiently in barley (Hordeum vulgare L. cv. Goseshikoku) by mechanical and biological stresses. One of the major antifungal compounds was identified as an indole alkaloid, gramine (N,N-dimethyl-3-aminomethylindole), by mass spectrum and NMR analyses. Gramine is well known as a constitutive compound of barley, but it increased significantly in the primary and secondary leaves of barley seedlings within 12 h after pruning or inoculating with the powdery mildew fungi of barley (Blumeria graminis Esp. hordei) and wheat (B. graminis Esp. tritici). However, in the leaf detached from unwounded seedlings or in the leaf inoculated with the barley powdery mildew fungus, gramine did not increase at all. In the water droplets contacted with barley leaves, the amount of leaked gramine increased dependently upon the time after the seedling was injured mechanically. We also found a tight correlation between gramine increase and enhancement of resistance to the barley powdery mildew fungus in barley leaves treated with an endogenous elicitor. Furthermore, such a systemic resistance was not observed in a barley cultivar Morex that lacks the biosynthetic pathway of gramine. From these results, we conclude that gramine is the excellent marker in rapid and transient systemic acquired resistance in barley.

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  • Generation of hydrogen peroxide is not required for harpin-induced apoptotic cell death in tobacco BY-2 cell suspension culture

    Y Ichinose, S Andi, R Doi, R Tanaka, F Taguchi, M Sasabe, K Toyoda, T Shiraishi, T Yamada

    PLANT PHYSIOLOGY AND BIOCHEMISTRY   39 ( 9 )   771 - 776   2001.9

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    To characterize molecular and biochemical mechanisms of hypersensitive reaction (HR) in plants, a tobacco suspension culture of BY-2 was treated with the proteinaceous HR elicitor harpin from several pathovars of Pseudomonas syringae. Tobacco BY-2 cells are sensitive to harpins derived from non-pathogenic pathovars of P. syringae, such as pvs. pisi, tomato and glycinea. These three harpins induce apoptotic cell death accompanied by DNA fragmentation in BY-2. Because the cell death was also accompanied by rapid generation of hydrogen peroxide (H2O2), one of the active oxygen species, we investigated the role of H2O2 in harpin-induced cell death. Although treatment with diphenylene iodium chloride (DPI) reduced, and catalase completely abolished, the harpin-induced generation of H2O2, the frequency of cell death was not affected at all. Treatment with superoxide dismutase (SOD) enhanced the generation of H2O2, but the cell death was unaffected. These results indicate that harpin-induced apoptotic cell death does not require oxidative burst. (C) 2001 Editions scientifiques et medicales Elsevier SAS

    DOI: 10.1016/S0981-9428(01)01300-6

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  • (325)Effect of Methyl Jasmonate on Harpin-Induced Hypersensitive Reaction in Tobacco

    Andi S., Taguchi F., Toyoda K., Shiraishi T., Ichinose Y

    Annals of the Phytopathological Society of Japan   67 ( 2 )   205 - 205   2001.8

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  • Molecular Cloning and Bacterial Expression of Extracellular Peroxidase from Pisum sativum : The Role in Superoxide Generation

    Nanba H., Toyoda K., Ichinose Y., Yamamoto M., Shiraishi T.

    Annals of the Phytopathological Society of Japan   67 ( 2 )   120 - 120   2001.8

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  • Characterization of Cell Wall Proteins that Interact with Plasma Membrane

    Ozaki H., Yuasa Y., Kiba A., Toyoda K., Ichinose Y., Yamamoto M., Shiraishi T.

    Annals of the Phytopathological Society of Japan   67 ( 2 )   120 - 121   2001.8

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  • Characterization of a Membrane-associated Protein Kinase that Phosphorylates Phosphatidylinositol-4-phosphate 5-kinase(PIP5K)

    Okazaki A., Toyoda K., Ichinose Y., Yamamoto M., Shiraishi T.

    Annals of the Phytopathological Society of Japan   67 ( 2 )   121 - 121   2001.8

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  • Plant Recognition and Response to Pathogens(2). Cloning of NTPase Genes from Host and Non-host plants

    Takahashi H., Kawahara T., Miura A., Yoshioka A., Toyoda K., Ichinose Y., Yamamoto M., Shiraishi T.

    Annals of the Phytopathological Society of Japan   67 ( 2 )   120 - 120   2001.8

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  • Use of Oxygen Meter to Detect Initial Response Induced in Strawberry Leaves by AF-toxin

    Yamamoto M., Toyoda K., Ichinose Y., Shiraishi T., Arai J.

    Annals of the Phytopathological Society of Japan   67 ( 2 )   123 - 123   2001.8

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  • Molecular Characterization of Dof DNA Binding Protein in Pea

    Nakamura M., Marutani M., Seki H., Toyoda K., Shiraishi T, Ichinose Y.

    Annals of the Phytopathological Society of Japan   67 ( 2 )   206 - 206   2001.8

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  • Oxidoredox Enzymes and Recognition/Response to Pathogens in Plant Cell Wall : Ascorbate Oxidase

    Osaki O., Kiba A., Toyoda K., Yamamoto M., Ichinose Y., Shiraishi T.

    Annals of the Phytopathological Society of Japan   67 ( 2 )   120 - 120   2001.8

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  • Hypersensitive Reaction-Inducing Factors Produced in Pseudomonas syringae(4)Structural and Functional Analysis of Flagellin

    Shimizu R., Taguchi F., Toyoda K., Shiraishi T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   67 ( 2 )   205 - 206   2001.8

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  • Effect of Harpin from Four Pathovars of Pseudomonas syringae on Pea Defense Responses (Short Communication) :

    TANAKA Rui, TAGUCHI Fumiko, ICHINOSE Yuki, TOYODA Kazuhiro, SHIRAISHI Tomonori, YAMADA Tetsuji

    Journal of general plant pathology : JGPP   67 ( 2 )   148 - 151   2001

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    To investigate the role of the proteinaceous elicitor, harpin, on host and nonhost plants, we isolated the harpin-coding gene, hrpZ, from Pseudomonas syringae pvs. pisi, glycinea, tabaci and tomato. Effects of the recombinant harpin proteins on pea plants were analyzed and compared with the effects of the corresponding bacterial treatment. After inoculation of pea with pea pathogen P. syringae pv. pisi, the bacterial population increased and the accumulation of PAL-mRNA and pisatin was inhibited. The nonpathogenic pathovars, glycinea, tabaci and tomato induced both defense responses in pea. However, none of the harpins induced the hypersensitive reaction or accumulation of PAL-mRNA and pisatin in pea. Harpins from P. syringae pvs. glycinea, tomato and pisi did induce these defense responses in tobacco, however, suggesting that externally applied harpins either are not recognized or are nonfunctional in pea plants. (Received July 27, 2000 ; Accepted February 21, 2001)

    DOI: 10.1007/PL00013001

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  • Effect of methyl jasmonate on harpin-induced hypersensitive cell death, generation of hydrogen peroxide and expression of defense genes in tobacco suspension cultured BY-2 cells.

    Andi, S, Taguchi, F, Toyoda, K, Shiraishi, T, Ichinose, Y

    Plant Cell Physiol.   42 ( 4 )   446 - 449   2001

  • Harpin_<psta> from Pseudomonas syringae pv. tabaci is defective and deficient in its expression and HR-inducing activity.

    TAGUCHI F.

    J. Gen. Plant Pathol.   67 ( 2 )   116 - 123   2001

  • 初期・表層シグナル伝達系と防御システム

    白石友紀, 豊田和弘, 木場章範, 一瀬勇規

    化学と生物   39 (10): 686-692 ( 10 )   686 - 692   2001

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    DOI: 10.1271/kagakutoseibutsu1962.39.686

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  • The Role of Active Oxygen Species Accompanied with HR by Elicitin in Tobacco Cell Suspension Culture(Abstracts Presented at the Meeting of the Kansai Division)

    Takeuchi K., Sasabe M., Ichinose Y., Toyoda K., Shiraishi T., Yamada T.

    Annals of the Phytopathological Society of Japan   66 ( 3 )   287 - 288   2000.12

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  • Hypersensitive Response-inducing Factors Produced in Pseudomonas syringae : (2) Induction and Suppression of Hypersensitive Reponse by Harpin(Abstracts Presented at the Meeting of the Kansai Division)

    Andi S., Taguchi F., Doi R., Tanaka R., Sasabe M., Toyoda K., Shiraishi T., Yamada T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   66 ( 3 )   292 - 293   2000.12

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  • Cell Wall NTPase in Pea Recognizes the Signal Molecules from Mycosphaerella pinodes(Abstracts Presented at the Meeting of the Kansai Division)

    Yoshioka K., Kiba A., Kawahara T., Toyoda K., Ichinose Y., Yamada T., Shiraishi T.

    Annals of the Phytopathological Society of Japan   66 ( 3 )   288 - 288   2000.12

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  • Hypersensitive Response-inducing Factors Produced in Pseudomonas syringae : (3) Isolation and Functional Analysis of Flagellin as an Elicitor Molecule(Abstracts Presented at the Meeting of the Kansai Division)

    Taguchi F., Nakajima R., Andi s., Toyoda K., Shiraishi T., Yamada T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   66 ( 3 )   293 - 293   2000.12

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  • Analysis of Suppressorresponsive Genes in Pea, : (3)Identification of S64 Protein as Oxophytodienoic Acid Reductase(Abstracts Presented at the Meeting of the Kansai Division)

    Ishiga Y., Ichinose Y., Toyoda K., Shiraisi T., Yamada T.

    Annals of the Phytopathological Society of Japan   66 ( 3 )   288 - 288   2000.12

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  • Interaction between an Integrin-like Protein(s) and a Phosphatidylinositol 4-Phosphate 5-Kinase (PIP5K) in Pea Plasma Membrane(Abstracts Presented at the Meeting of the Kansai Division)

    Toyoda K., Okazaki A., Ichinose Y., Yamada T., Shiraishi T.

    Annals of the Phytopathological Society of Japan   66 ( 3 )   288 - 288   2000.12

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  • Hypersensitive Response-Inducing Factors Produced in Pseudomonas syringae : (1) Structural and Functional Analysis of Harpin Pseudomonas syringae(Abstracts Presented at the Meeting of the Kansai Division)

    Tanaka R., Taguchi F., Andi S., Kinoshita S., Toyoda K., Shiraishi T., Yamada T., Ichinose Y.

    Annals of the Phytopathological Society of Japan   66 ( 3 )   292 - 292   2000.12

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  • Potentiation of phytoalexin accumulation in elicitor-treated epicotyls of pea (Pisum sativum) by a diacylglycerol kinase inhibitor

    K Toyoda, T Kawahara, Y Ichinose, T Yamada, T Shiraishi

    JOURNAL OF PHYTOPATHOLOGY   148 ( 11-12 )   633 - 636   2000.12

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    When epicotyl tissues of pea were treated with a diacylglycerol (DAG) kinase inhibitor (R59022), enhanced induction of the phytoalexin accumulation occurred which was induced by fungal elicitor. The marked induction was associated with a sustained accumulation of phenylalanine ammonia-lyase (PAL)-mRNA and the consequent increase in PAL activity. These results suggest that inhibition of DAG breakdown leads to increased induction of phytoalexin accumulation and support the hypothesis that DAG kinase negatively regulates the signal transduction.

    DOI: 10.1046/j.1439-0434.2000.00568.x

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  • Specific Inhibition of Cell Wall-bound ATPase by the Suppressor from Mycosphaerella pinodes(6) : Cloning and Functional Analysis of Fungal Signal-binding Protein from Pea Plant

    Kiba A., Ohgawara T., Toyoda K., Ichinose Y., Yamada T., Shiraishi T.

    Annals of the Phytopathological Society of Japan   66 ( 2 )   116 - 117   2000.8

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  • Cytochalasin A inhibits the binding of phenylalanine ammonia-lyase mRNA to ribosomes during induction of phytoalexin in pea seedlings

    M Sugimoto, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

    PLANT AND CELL PHYSIOLOGY   41 ( 2 )   234 - 238   2000.2

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    Cytochalasin A (CA) blocked the accumulation of phytoalexin and phenylalanine ammonia-lyase (PAL)-protein in pea tissues treated with a fungal elicitor but scarcely affected the PAL-mRNA content. Further analysis showed that CA decreased the PAL-mRNA bound to ribosomes. These results indicate that actin filaments are tightly associated with the translational process of the PAL gene.

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  • Genes expressed in Ascochyta rabiei-inoculated chickpea plants and elicited cell cultures as detected by differential cDNA-hybridization

    Y Ichinose, K Tiemann, C Schwenger-Erger, K Toyoda, F Hein, T Hanselle, H Cornels, W Barz

    ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES   55 ( 1-2 )   44 - 54   2000.1

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    In response to the exogenous application of elicitors and attempted invasion by pathogens, plants exhibit a wide range of defense reactions. To understand the defense mechanisms at the level of gene activation and deactivation, differential screenings were performed to isolate cDNA clones which are differentially expressed in pathogen-inoculated resistant chickpea plants and elicitor-treated cell cultures. A plenty of genes were isolated and arranged in 5 groups, namely defense-related pathways, signal transduction pathways, regulation of gene expression, catabolic pathways and primary metabolism. Most of these genes were activated although several genes were also found to be suppressed. We discuss the plausible functions of cDNA products in plant defense responses. The cDNAs provide a variety of tools to investigate molecular mechanisms of defense responses and clearly reflect the massive genomic and metabolic changes which occur during manifestation of antimicrobial defense.

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  • Importance of AC-rich element on pea phenylalanine ammonia-lyase gene 1 promoter for expression induced by nonpathogenic attack

    IMURA Yoshiyuki, IGUCHI Satoko, TOYODA Kazuhiro, ICHINOSE Yuki, SHIRAISHI Tomonori, YAMADA Tetsuji

    Journal of general Plant Pathology   66 ( 2 )   123 - 127   2000

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    Regulatory elements in the promoter of phenylalanine ammonia-lyase gene 1 of pea(PSPAL1)in response to nonpathogenic attack were identified by in vivo footprinting analysis. The footprints determined AC-rich sequences, Box-I and Box-II, that were conserved at similar positions in the phenylpropanoid gene promoters from several plants. To reveal the functions of the AC-rich sequence in nonpathogen-responsiveness, we constructed Box-I-deletion PSPAL1 promoter(dB-1)with GUS reporter gene and transformed it into tobacco plant. The dB-1 had reduced basal expression and a complete loss of nonpathogen-responsiveness. These results indicate the essentiality of Box-I for PSPAL1 activation induced by nonpathogenic attack.

    DOI: 10.1007/PL00012932

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  • PLANT CELL WALL RECOGNIZES AND RESPONDS TO SIGNALS FROM PHYTOPATHOGENS :

    KIBA Akinori, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

    Plant and cell physiology   41   s10   2000

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  • INDEPENDENT PATHWAYS OF DEFENSE RESPONSES IN ELICITIN-TREATED TOBACCO BY-2 CELLS :

    SASABE Michiko, TAKEUCHI Kasumi, KAMOUN Sophien, ICHINOSE Yuki, TOYODA Kazuhiro, SHIRAISHI Tomonori, YAMADA Tetsuji

    Plant and cell physiology   41   s85   2000

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  • STRUCTURAL AND FUNCTIONAL ANALYSES OF HARPINS FROM SEVERAL PATHOVARS OF PSEUDOMONAS SYRINGAE :

    TANAKA Rui, TAGUCHI Tomiko, KINOSHITA Sayuri, NAKADA Hideki, ICHINOSE Yuki, TOYODA Kazuhiro, SHIRAISHI Tomonori, YAMADA Tetsuji

    Plant and cell physiology   41   s84   2000

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  • Analysis of Suppressorresponsive Genes in Pea (2) : Molecular Structure and Gene Expression of S64 (Abstracts Presented at the Meeting of the Kansai Division)

    Funato A., Ishiga Y., Ichinose Y., Toyoda K., Shiraishi T., Yamada T., Barz W.

    Annals of the Phytopathological Society of Japan   65 ( 6 )   671 - 671   1999.12

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  • Induction of Resistance against Powdery Mildew in Barley Leaves by Volatiles from Tomato Seedlings (Abstracts Presented at the Meeting of the Kansai Division)

    Watanabe T., Takabayashi J., Hiramoto T., Toyoda K., Ichinose Y., Yamada T., Shiraishi T.

    Annals of the Phytopathological Society of Japan   65 ( 6 )   671 - 672   1999.12

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  • Fungal Signals and Their Perception in Plant Cell Walls : Regulation of Diamine Oxidase Activity in Isolated Cell Wall from Pea Seedlings by an Elicitor and a Suppressor from Mycosphaerella pinodes (Abstracts Presented at the Meeting of the Kansai Division)

    Ohwatari Y., Watanabe Y., Toyoda K., Ichinose Y., Yamada Y., Shiraishi T.

    Annals of the Phytopathological Society of Japan   65 ( 6 )   671 - 671   1999.12

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  • Induction of Hypersensitive Cell Death and Defense Gene Expression by Elicitin in Tobacco Cell Suspension Culture (Abstracts Presented at the Meeting of the Kansai Division)

    Sasabe M., Takeuchi K., Kamoun S., Ichinose Y., Toyoda K., Shiraishi T., Yamada T.

    Annals of the Phytopathological Society of Japan   65 ( 6 )   671 - 671   1999.12

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  • cDNA cloning and gene expression of three small GTP-binding proteins in defense response of chickpea

    Y Ichinose, K Toyoda, W Barz

    BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION   1489 ( 2-3 )   462 - 466   1999.12

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    The cDNA clones encoding rab type (INR134 and ELR19) and rac type (ELR26) small GTP-binding proteins were isolated from Ascochyta rabiei-inoculated chickpea leaves and the elicitor-treated cell cultures. Rac type ELR26 showed enhanced expression in inoculated leaves indicating correlation with the defense response. (C) 1999 Elsevier Science B.V. All rights reserved.

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  • Induction of defense responses by synthetic glycopeptides that have a partial structure of the elicitor in the spore germination fluid of Mycosphaerella pinodes

    A Kiba, T Takeda, T Kanemitsu, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

    PLANT AND CELL PHYSIOLOGY   40 ( 9 )   978 - 985   1999.9

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    A high molecular weight elicitor (&gt;70 kDa) from spore germination fluid of a pea pathogen, Mycosphaerella pinodes, has a partial structure of beta-D-Glc-(1--&gt;6)-alpha-D-Man-(1--&gt;6)-D-Man, which is O-glycosidically attached to serine in the protein moiety. To elucidate the minimum structure for the elicitor activity to pea plants, the effects of nine glycopeptides including beta-D-Glc-(1--&gt;6)-alpha-D-Man-(1--&gt;6)-D-Man-O-Ser (No. 1) to [beta-D-Glc-(1--&gt;6)-alpha-D-Man-(1--&gt;6)-D-Man](3)-O-Ser(3)-Pro(3) (No. 9) on the infection by M. pinodes, superoxide generation and ATPase activity were measured. The glycopeptides [beta-D-Glc-(1--&gt;6)-alpha-D-Man-(1--&gt;6)-D-Man]-O-Ser(2)-Pro(2) (No. 3) to No. 9 induced rejection reaction of pea tissue against M. pinodes. The glycopeptides No. 3 to No. 9 also induced superoxide generation on uninjured pea leaves. Moreover, the glycopeptides No. 3 to No. 9 induced in vitro the activation of cell wall-bound ATPase and superoxide generation system in the protein fraction solubilized from pea cell wall. The results indicate that the synthetic glycopeptides, No. 3 to No. 9, are available to analyze the signal transduction cascade leading to defense responses and the receptor for the elicitor.

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  • Isolation and Structural Analysis of Elicitor-responsive Gene in Pea : (4) : Analysis of hsr203J Homolog in Pea

    Hisayasu Y., Ichinose Y., Toyoda K., Shiraishi T., Yamada T.

    Annals of the Phytopathological Society of Japan   65 ( 3 )   325 - 325   1999.6

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  • Functional Analysis of Cis-regulatory Elements in PSPAL1 and Application to Molecular Breeding of Anti-bacterial Plants

    Imura Y., Fujiwara T., Ozawa H., Tanaka H., Iguchi S., Toyoda K., Ichinose Y., Shiraishi T., Yamada T.

    Annals of the Phytopathological Society of Japan   65 ( 3 )   372 - 373   1999.6

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  • Analysis of Dof DNA Binding Protein Which was Induced by Wounding and Elicitor in Pea

    Seki H., Ichinose Y., Toyoda K., Shiraishi T., Yamada T.

    Annals of the Phytopathological Society of Japan   65 ( 3 )   325 - 325   1999.6

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  • Involvement of Actin Filaments in Defense Response of Pea

    Sugimoto M., Toyoda K., Kiba A., Ichinose Y., Yamada T., Shiraishi T.

    Annals of the Phytopathological Society of Japan   65 ( 3 )   325 - 325   1999.6

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  • Integrin-like Protein(s) May Interact with Polyphosphoinositide Metabolism during Induction of Pea Defense Response

    Toyoda K., Kiba A., Sugimoto M., Ichinose Y., Yamada T., Shiraishi T.

    Annals of the Phytopathological Society of Japan   65 ( 3 )   324 - 325   1999.6

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  • Co-purification of plasma membrare ATPase and phosphatidylinositol kinase from pea plasma membrane

    Scientific Reports of the Faculty of Agriculture Okayama University   88   31 - 38   1999

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  • (32) Specific Inhibition of Cell Wall-bound ATPase by the Suppressor from Mycosphaerella pinodes (6) Elicitor- and Suppressor-binding Proteins in ATPase Fraction from Cowpea Cell Wall (Abstracts Presented at the Meeting of the Kansai Division, 1998)

    Yamashita H., Kiba A., Toyoda K., Ichinose Y., Yamada T., Shiraishi T.

    Annals of the Phytopathological Society of Japan   64 ( 6 )   623 - 623   1998.12

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  • (13) Induction of Superoxide-scavenging Enzyme-mRNAs in Fungal Elicitor-treated Cells of Chickpea (Cicer arietinum) (Abstracts Presented at the Meeting of the Kansai Division, 1998)

    Toyoda K., Ichinose Y., Shiraishi T., Yamada T., Barz W.

    Annals of the Phytopathological Society of Japan   64 ( 6 )   618 - 618   1998.12

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  • (8) Analysis of Signal Transmission between Cell Wall and Plasma Membrane (2) Interaction between Cell Wall and Plasma Membrane Is Implicated in Defense Responses of Pea Plant (Abstracts Presented at the Meeting of the Kansai Division, 1998)

    Kiba A., Sugimoto M., Azuma T., Yuasa Y., Toyoda K., Ichinose Y., Yamada T., Shiraishi T.

    Annals of the Phytopathological Society of Japan   64 ( 6 )   617 - 617   1998.12

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  • (9) Identification of Cis-regulatory Elements Involved in the Defense-associated and Organ-specific Expression of Pea PAL Genes by in vivo Footprinting Analysis (Abstracts Presented at the Meeting of the Kansai Division, 1998)

    Imura Y., Seki H., Toyoda K., Ichinose Y., Shiraishi T., Yamada T.

    Annals of the Phytopathological Society of Japan   64 ( 6 )   617 - 617   1998.12

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  • (12) Characterization of Genes Expressed in Ascochyta rabiei-inoculated Chickpea Plants and Elicited Cell Cultures (Abstracts Presented at the Meeting of the Kansai Division, 1998)

    Ichinose Y., Tiemann K., Schwenger-Erger C., Toyoda K., Hein F., Yamada T., Shiraishi T., Barz W.

    Annals of the Phytopathological Society of Japan   64 ( 6 )   618 - 618   1998.12

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  • (7) Involvement of Actin Filaments in Defense Response of Pea (Abstracts Presented at the Meeting of the Kansai Division, 1998)

    Sugimoto M., Toyoda K., Kiba A., Ichinose Y., Yamada T., Shiraishi T.

    Annals of the Phytopathological Society of Japan   64 ( 6 )   616 - 617   1998.12

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  • Analysis of a gene encoding D of DNA binding protein which was induced by wounding and elicitor in pea.

    SEKI H., ICHINOSE Y., OKABE T., SANEMATSU S., TOYODA K., SHIRAISHI T., YAMADA T.

    21   433 - 433   1998.12

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  • Interaction between cell wall and plasma membrane via RGD motif is implicated in plant defense responses

    A Kiba, M Sugimoto, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

    PLANT AND CELL PHYSIOLOGY   39 ( 11 )   1245 - 1249   1998.11

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    Integrin- and vitronectin-like proteins were found to exist in the pea plasma membrane and cell wall, respectively. The hexapeptide GRGDSP but not GRGESP inhibited either the binding of cell wall protein(s) to plasma membrane protein(s) or the defense response. A possible role of linkage via RGD motif in plant defenses is discussed.

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  • (123) Isolation and Structural Analysis of Elicitor-responsive Genes in Pea (3) Characterization for a Novel Putative Transcription Factor Possessing Dof Domain

    Okabe T., Ichinose Y., Seki H., Toyoda K., Shiraishi T., Yamada T.

    Annals of the Phytopathological Society of Japan   64 ( 4 )   357 - 358   1998.8

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  • (122) Specific Inhibition of Cell Wall-bound ATPase by the Suppressor from Mycosphaerella Pinodes (5) Analysis of Elicitor- and Suppressor-binding Proteins in Pea Cell Wall Fraction

    Kiba A., Sugimoto M., Toyoda K., Ichinose Y., Yamada T., Shiraishi T.

    Annals of the Phytopathological Society of Japan   64 ( 4 )   357 - 357   1998.8

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  • In vivo FOOTPRINTING ANALYSIS OF cis-REGULATORY ELEMENTS INVOLVED IN THE ELICITOR-MEDIATED ACTIVATION AND SUPPRESSOR-MEDIATED DEACTIVATION OF PEA PAL AND CHS

    SEKI Hikaru, ICHINOSE Yuki, NAGASUGI Yumi, TOYODA Kazuhiro, SHIRAISHI Tomonori, YAMADA Tetsuji

    39   S43 - S143   1998.5

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  • ISOLATION AND CHARACTERIZATION OF ELICITOR-RESPONSIVE GENES IN PEA

    ICHINOSE Yuki, SANEMATSU Shiroh, ENDOH Ai, OKABE Takeharu, HISAYASU Yumiko, SEKI Hikaru, SHIRAISHI Tomonori, TOYODA Kazuhiro, YAMADA Tetsuji

    39   S143 - S143   1998.5

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  • PURIFICATION AND ANALYSIS OF ELICITOR BINDING-PROTEIN IN CELL WALL FRACTION FROM PEA

    SUGIMOTO Megumi, KIBA Akinori, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

    39   S143 - S143   1998.5

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  • SPECIFIC REGULATION OF CELL WALL-BOUND ATPASE BY THE SUPPRESSOR FROM MYCOSPHAERELLA PINODES(3) -IDENTIFICATION OF ELICITOR- AND SUPPRESSOR-BINDING PROTEINS IN CELL WALL-BOUND ATPASE FRACTION FROM PEA PLANT-

    KIBA Akinori, SUGIMOTO Megumi, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

    39   S144 - S144   1998.5

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  • RECOGNITION OF FUNGAL SUPPRESSOR BY PEA PLANTS II

    SUGIURA Tetsuya, KIBA Akinori, AOYAGI Masaaki, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

    39   S144 - S144   1998.5

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  • Phosphorylation of Phosphatidylinositols and Production of Lysophospholipid in Pea Plasma Membrane Are Coordinately Regulated by Elicitor and Suppressor from Mycosphaerella pinodes

    Toyoda Kazuhiro, Koyama Masashi, Mizukoshi Rumi, Ichinose Yuki, Yamada Tetsuji, Shiraishi Tomonori

    87 ( 1 )   109 - 116   1998.2

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  • Molecular cloning of a cDNA encoding a putative DNA-binding zinc-finger protein in pea

    Scientific Reports of the Faculty of Agriculture Okayama University   88   25 - 30   1998

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  • Elevation of diacylglycerol during the early stage of elicitor-signal transduction in Pea(Pisum sativum)

    Kazuhiro TOYODA, Tomoharu KAWAHARA, Rumi MIZUKOSHI, Masashi KOYAMA, Yuki ICHINOSE, Tetsuji YAMADA, Tomonori SHIRAISHI

    Annals of the phytopathological Society of Japan   64 ( 5 )   485 - 487   1998

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    DOI: 10.3186/jjphytopath.64.485

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  • Plant cell wall my recognize pathogenic microorganisms

    Tomonori SHIRAISHI, Akinori KIBA, Kazuhiro TOYODA, Yuki ICHINOSE, Tetsuji YAMADA

    KASEAA   36 ( 4 )   226 - 233   1998

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    DOI: 10.1271/kagakutoseibutsu1962.36.226

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  • (9) Analysis of Suppressor-responsive Genes in Pea : (1) Isolation of the cDNAs by Differential Hybridization

    TACHIKI T., ICHINOSE Y., TOYODA K., SHIRAISHI T., YAMADA T.

    Annals of the Phytopathological Society of Japan   63 ( 6 )   509 - 509   1997.12

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  • (8) Isolation and Structural Analysis of Elicitor-responsive Genes in Pea (2)

    ICHINOSE Y., SANEMATSU S., ENDOH A., OKABE T., HISAYASU Y., SEKI H., TOYODA K., SHIRAISHI T., YAMADA T.

    Annals of the Phytopathological Society of Japan   63 ( 6 )   508 - 509   1997.12

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  • (7) Isolation and Structural Analysis of Elicitor-responsive Genes in Pea (1)

    SANEMATSU S., ICHINOSE Y., ENDOH A., TOYODA K., SHIRAISHI T., YAMADA T.

    Annals of the Phytopathological Society of Japan   63 ( 6 )   508 - 508   1997.12

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  • (6) Specific Inhibition of Cell Wrall-bound ATPase by the Suppressor from Mycospharella pinodes. : (IV) Identincation of Elicitor- and Suppressor-binding Proteins in Cell Wrall-bound ATPase-rich Fraction from Pea Plants

    KIBA A., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

    Annals of the Phytopathological Society of Japan   63 ( 6 )   508 - 508   1997.12

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  • (69) Nucleotide Sequence of the Coat Protein Gene of Tobamoviruses Isolated from Capsicum spp. Containing L3 Gene

    HASHIMURA K., TAKEUCHI S., MIZUMOTO H., HAMADA H., OKUMURA A., KURODA T., TOYODA K., SUZUKI K., HIKICHI Y., OKUNO T.

    Annals of the Phytopathological Society of Japan   63 ( 6 )   523 - 524   1997.12

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  • 特異性決定に関わる分子スイッチ

    白石 友紀, 木場 章範, 豊田 和弘, 一瀬 勇規, 山田 哲治

    日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan   61   71 - 71   1997.9

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  • Con A-binding protein in plasma membranes may participate in signal-transduction cascades that lead to defense responses in pea.

    K Toyoda, M Sugimoto, T Kawahara, Y Ichinose, T Yamada, T Shiraishi

    PLANT PHYSIOLOGY   114 ( 3 )   1502 - 1502   1997.7

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  • Specific regulation of cell wall-bound ATPase and peroxidase by the suppressor for defense response.

    A Kiba, A Inata, C Miyake, K Toyoda, Y Ichinose, T Yamada, T Shiraishi

    PLANT PHYSIOLOGY   114 ( 3 )   1176 - 1176   1997.7

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  • Mechanisms of the planet disease resistance : Focusing on the suppressor

    YAMADA Tetsuji, SHIRAISHI Tomonori, ICHINOSE Yuki, TOYODA Kazuhiro

    Chemical regulation of plants   32 ( 1 )   49 - 59   1997.6

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  • (269) Infection Route of CMV in Gentian : 6) Spread of CMV Infection in Fields(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    SUZUKI K., OKUMURA A., NASU Y., TOYODA K., HIKICHI Y.

    Annals of the Phytopathological Society of Japan   63 ( 3 )   258 - 258   1997.6

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  • (204) Synchronized Multiplication of Pseudomonas cichorii and Protein Synthesis in Lettuce Leaf(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    HIKICHI Y., HORIKOSHI M., HIROOKA T., TOYODA K., SUZUKI K., OKUNO T.

    Annals of the Phytopathological Society of Japan   63 ( 3 )   242 - 242   1997.6

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  • (207) Behavior of Bioluminescent Ralstonia solanacearum Transformed by luxCDABE in Bacterial Wilt Resistant Plants(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    NASU Y., HORIKOSHI M., HIROOKA T., TOYODA K., SUZUKI K., OKUNO T., HIKICHI Y.

    Annals of the Phytopathological Society of Japan   63 ( 3 )   242 - 243   1997.6

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  • (271) Epidemiological Studies on the Japanese Tobamovirus Strains Infecting the L^2-resistance Genotypes of Pepper, a Member of Pepper Mild Mottle Virus (PMMV) Sub-group : II. Are Infected Seeds the Initial Sources of Viral Infection?(Abstracts of the Papers Presented at the Annual Meeting of the Society)

    TOYODA K., OKUMURA A., NASU Y., HIKICHI Y., OKUNO T., SUZUKI K.

    Annals of the Phytopathological Society of Japan   63 ( 3 )   258 - 259   1997.6

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  • Orthovanadate Induces Phytoalexin Production in Pea Suspension-Cultured Cells

    Kawahara Tomoharu, Toyoda Kazuhiro, Kiba Akinori, Ichinose Yuki, Yamada Tetsuji, Shiraishi Tomonori

    Scientific reports of the Faculty of Agriculture, Okayama University   86   33 - 41   1997.2

  • The role of suppressors in determining host-parasite specificities in plant cells

    T Shiraishi, T Yamada, Y Ichinose, A Kiba, K Toyoda

    INTERNATIONAL REVIEW OF CYTOLOGY - A SURVEY OF CELL BIOLOGY, VOL 172   172   55 - 93   1997

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  • Superoxide generation in extracts from isolated plant cell walls is regulated by fungal signal molecules.(共著)

    KIBA A, MIYAKE C, TOYODA K, ICHINOSE Y, YAMADA T, SHIRAISHI T

    Phytopathology   87 ( 8 )   846 - 852   1997

  • Nucleotide Sequence of the Coat Protein Gene of Pepper Mild

    TOYODA K., OKUMURA A., NASU Y., HIKICHI Y., SUZUKI K.

    Annals of the Phytopathological Society of Japan   62 ( 6 )   598 - 598   1996.12

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  • Bioluminescence of Pseudomonas solanacearum Transformed with luxCDABEOperon in Tomato Plants

    NASU Y., HIKICHI Y., HORIKOSHI M., OKUMURA A., TOYODA K., HIROOKA T., SUZUKI K.

    Annals of the Phytopathological Society of Japan   62 ( 6 )   605 - 605   1996.12

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  • Relationship between Multiplication of Pseudomonas cichorii in Vascular Bundle of Lettuce Leaves and Symptom Development of Bacterial Rot of Lettuce

    HIKICHI Y., NASU Y., HORIKOSHI M., OKUMURA A., TOYODA K., HIROOKA T., SUZUKI K.

    Annals of the Phytopathological Society of Japan   62 ( 6 )   605 - 605   1996.12

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  • 植物の病原体認識とシグナル伝達 (植物のシグナルトランスダクション--分子機構と実験法) -- (細胞認識のシグナリング)

    豊田 和弘, 白石 友紀, 山田 哲治

    現代化学増刊   ( 30 )   185 - 194   1996.11

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  • 植物による病原菌の認識機構

    白石 友紀, 山田 哲治, 一瀬 勇規, 木場 章範, 豊田 和弘

    日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan   60   71 - 71   1996.10

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  • 植物レクチンコンカナバリンAによるエリシター結合蛋白質の分離

    豊田 和弘, 杉本 恵, 一瀬 勇規, 山田 哲治, 白石 友紀

    日本分子生物学会年会プログラム・講演要旨集   19   631 - 631   1996.8

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  • 生物発光Pseudomonas cichoriiのレタス葉における挙動

    曳地 康史, 那須 佳子, 奥村 亜子, 豊田 和弘, 鈴木 一実, 堀越 守, 廣岡 卓

    日本分子生物学会年会プログラム・講演要旨集   19   423 - 423   1996.8

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  • Involvement of Active Oxygen Species during the Plantpathogen Interaction : (II) Specific Inhibition of Superoxide Generation in Plant Cell Wall by Fungal Suppressor

    KIBA A., MIYAKE C., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

    Annals of the Phytopathological Society of Japan   62 ( 3 )   292 - 292   1996.6

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  • Recognition of Suppressor from Mycosphaerella pinodes by Pea Plants : Analysis of Supprescin A-binding Molecule (s) in Pea Cell Wall by RMD

    SUGIURA T., AOYAGI M., KIBA A., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

    Annals of the Phytopathological Society of Japan   62 ( 3 )   292 - 292   1996.6

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  • Characterization of Concanavalin A-binding Protein Complex from Pea Plasma Membranes : (III) An Actin-binding Protein in Con A-binding Protein Complex as Determined by Far Western Blotting with a Biotinylated Actin

    TOYODA K., SUGIMOTO M., ICHINOSE Y., YAMADA T., SHIRAISHI T.

    Annals of the Phytopathological Society of Japan   62 ( 3 )   290 - 290   1996.6

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  • SUPEROXIDE GENERATION IN CELL WALL DURING A PLANT-PATHOGEN INTERACTION

    KIBA Akinori, MIYAKE Chizu, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

    37   127 - 127   1996.3

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  • PURIFICATION AND CHARACTERIZATION OF A BINDING PROTEIN FOR CONCANAVALIN A THAT ELICITS PHYTOALEXIN PRODUCTION IN PEA(PISUM SATIVUM L. )

    TOYODA Kazuhiro, SUGIMOTO Megumi, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

    37   15 - 15   1996.3

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  • ASSOCIATION BETWEEN DEFENSE RESPONSES AND ION-FLUX(II)

    AMANO Masashi, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

    37   57 - 57   1996.3

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  • Recognition of Pathogens and Signal Transduction in Higher Plants

    Tokyo kagaku dojin   185 - 194   1996

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  • 植物防御応答にかかわるシグナル伝達機構-病原菌シグナルによる制御-(共著)

    植物感染生理学の現状と将来展望日本植物病理学会   131 - 140   1996

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  • 植物の病原体認識とシグナル伝達(共著)

    現代化学・増刊30植物のシグナルトランスダクション-分子機構と実験法-東京化学同人   185 - 194   1996

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  • Speciesspecific suppression of superoxide-anion generation of surfaces of pea leaves by the suppressor from Mycosphaerella pinodes

    KIBA A.

    Ann. Phytopathol. Sco. Jpn.   62 ( 5 )   508 - 512   1996

  • Signal Transduction Pathways of Higher Plants Are Regulated by Fungal Signals

    Actual and Future Perspectives in Physiological Plant Pathology, The Phytopathological Society of Japan   131 - 140   1996

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  • Regulation of Defense Responses in Pea by Fungal Singals, Elicitor and Suppressor from Mycosphaerella pinodes : 9 Involvement of diacylglycerol during Defense Responses

    KAWAHARA T., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

    Annals of the Phytopathological Society of Japan   61 ( 6 )   613 - 613   1995.12

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  • Involvement of Active Oxigen Species during the Plant-pathogen Interaction : Specific Suppression of Superoxide Generation by the Suppressor from Mycosphaerella pinodes

    KIBA A., YAMAGUCHI Y., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

    Annals of the Phytopathological Society of Japan   61 ( 6 )   612 - 612   1995.12

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  • Inhibition of a Phytoalexin Accumulation in Pea Eepicotyl Tissues by Cytochalasins

    SUGIMOTO M., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

    Annals of the Phytopathological Society of Japan   61 ( 6 )   613 - 613   1995.12

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  • The Role of Sugar Moieties of Elicitor and Suppressor in Plant-fungal Parasite Recognition : 3 Effect of Galactose on the Accumulation of Pisatin

    YOSHIMOTO Y., TOYODA K., KATO T., ICHINOSE Y., YAMADA T., SHIRAISHI T.

    Annals of the Phytopathological Society of Japan   61 ( 6 )   614 - 614   1995.12

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  • Characterization of Concanavalin A-binding protein Complex from Pea Plasma Membranes : II Phosphatidylinositol Kinase, Plasma Membrane ATPase, Small GTP-binding Proteins, Nucleoside Diphosphate Kinase G-actin and Certain Catalytic Subunits of Protein Kinase C are Associated with the Binding Protein of Concanavalin A That Elicits a Defense Response in Pea

    TOYODA K., SUGIMOTO M., KAMEI A., ICHINOSE Y., YAMADA T., SHIRAISHI T.

    Annals of the Phytopathological Society of Japan   61 ( 6 )   614 - 614   1995.12

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  • Effect of Suppressor from Mycosphaerella pinodes on the ATPase Activity in Microsomal Fraction from the Fungus

    MISE D., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

    Annals of the Phytopathological Society of Japan   61 ( 6 )   615 - 615   1995.12

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  • Relation between Defense Responses and Ion Flux : III. Rapid Change in Ion-flux on Cowpea Tissues Treated with the Suppressor from Mycosphaerella pinodes

    AMANO M., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

    Annals of the Phytopathological Society of Japan   61 ( 6 )   612 - 612   1995.12

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  • H^+-translocating Activity in Proteoliposomes Reconstituted with Pea Plasma Membrane ATPase and Its Inhibition by Fungal Suppressor from Mycosphaerella pinodes

    AMANO Masashi, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

    Annals of the Phytopathological Society of Japan   61 ( 4 )   369 - 375   1995.8

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    Effects of the elicitor and the suppressor from a pea pathogen, Mycosphaerella pinodes, on the H^+-translocating activity of ATPase in pea plasma membranes were examined. The plasma membrane ATPase was solubilized with Triton X-100 and partially purified by continuous glycerol density gradient centrifugation. The ATPase fraction was reconstituted into soybean phospholipid (asolectin) liposomes by a Triton-X 100 dilution method. Almost all of resultant proteoliposome vesicles had unimembranes in size from 50 to 200 nm. The ATP-driven H^+-pumping activity was measured by quinacrine fluorescence quenching in the presence of Mg-<2+>-ATP. The activity was sensitive to orthovanadate, dicyclohexylcar-bodiimide (DCCD), verapamil and neomycin but it was insensitive to azide and nitrate as well as ATP-hydrolyzing activity. Proton gradient was collapsed by NH_4Cl or a channel-forming ionophore, gramicidin D. The H^+-pumping activity in proteoliposomes was hardly affected by the elicitor. The finding suggests that a putative target molecule of or receptor for the elicitor might not associated with the solubilized ATPase or that the elicitor could not reach the target site. However, the suppressor markedly inhibited both activities in proteoliposomes, showing that the H^+-translocating ATPase might be inhibited directly in vitro by the suppressor as reported previously and that the fungal suppressor may disturb the regulation of pH in the host cells. Both activities were increased by the addition of phosphatidylinositolbisphosphate, even if in the presence of the suppressor. However, PIP_2 could not completely negate the effect of the suppressor. These results suggest that the H^+-pumping activity of plasma membrane ATPase is also regulated by polyphosphoinositide metabolism and that the action sites of the suppressor on the ATPase may be different from those of PIP_2.

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    Other Link: http://agriknowledge.affrc.go.jp/RN/2010521200

  • PLANT-LECTINS INDUCE THE PRODUCTION OF A PHYTOALEXIN IN PISUM-SATIVUM

    K TOYODA, K MIKI, Y ICHINOSE, T YAMADA, T SHIRAISHI

    PLANT AND CELL PHYSIOLOGY   36 ( 5 )   799 - 807   1995.7

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    The effects of several plant lectins on the production of a pea phytoalexin, pisatin, were examined. Con A, PHA, PNA and PSA each induced the production of pisatin in pea epicotyl tissues, demonstrating that plant lectins can act as elicitors. The production of pisatin in response to PHA, PNA or PSA was not affected by the simultaneous presence of the respective hapten sugars, whereas haptens specific for Con A, such as alpha-D-mannose and methyl-alpha-D-mannoside, abolished the induction of pisatin by Con A. These results indicate that the elicitor effect of Con A is attributable to its ability to bind to specific carbohydrates in pea cells. Induction of the production of pisatin by Con A was markedly inhibited by the suppressor derived from a pea pathogen. Mycosphaerella pinodes, and by several inhibitors related to signal-transduction pathways. It is suggested, therefore, that the Con A-induced production of pisatin in pea tissues might be associated with activation of a signal-transduction pathway. An additive effect on the accumulation of pisatin was observed when Con A was present with a polysaccharide elicitor from M. pinodes, suggesting that exogenous Con A does not compete with the recognition site(s) for the fungal elicitor in pea cells. The present data also indicate that Con A may be useful for characterization of the signal-transduction system that leads to the synthesis of phytoalexin in pea epicotyl tissues.

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  • SPECIFIC-INHIBITION OF CELL WALL-BOUND ATPASES BY FUNGAL SUPPRESSOR FROM MYCOSPHAERELLA-PINODES

    A KIBA, K TOYODA, Y ICHINOSE, T YAMADA, T SHIRAISHI

    PLANT AND CELL PHYSIOLOGY   36 ( 5 )   809 - 817   1995.7

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    Activities of phosphatases were found in the fractions which were solubilized from cell walls of both pea and cowpea seedlings with 0.5 M NaCl. These phosphatases hydrolyzed triphosphonucleotides in the order: UTP = CTP &gt; GTP &gt; ATP; and UTP = GTP &gt; CTP = ATP, respectively. The activities of a pyrophosphatase and a p-nitrophenylphosphatase were also detected in these fractions. The suppressor in the spore germination fluid of a pea pathogen, Mycosphaerella pinodes, inhibited all of these phosphatase activities in the fraction solubilized from pea cell walls, but it rather enhanced only the activity of the ATPase among those phosphatases from the cowpea cell wall. Hydrolysis of ATP by a cell wall fraction of pea was also markedly inhibited by the suppressor, while hydrolysis of ATP by similar fractions from cowpea, kidney bean and soybean were rather enhanced by the suppressor, as well as by the elicitor. Thus, the cell wall-bound ATPases responded to the suppressor species-specifically. These cell wall-bound ATPases seemed to be different from the plasma membrane ATPases in several respects. The results suggest that plants recognize the fungal signals not only on their plasma membranes but also on their cell walls and, moreover that putative receptors for the fungal signals might be located close to cell wall-bound ATPases or might even be these ATPases themselves.

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  • Relationship between Defense Responses and Ion Flux : (II) Papid Na^+-Flux in Pea Tissues Treated with the Elicitor

    AMANO M., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

    Annals of the Phytopathological Society of Japan   61 ( 3 )   247 - 247   1995.6

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  • Specific Inhibition of Cell Wall-Bound ATPase by the Suppressor from Pea Pathogen mYCOSHAERELLA PINODES : (3) Effect of the Suppressor on ATPase Partially Purifed from Cell Walls

    KIBA A., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

    Annals of the Phytopathological Society of Japan   61 ( 3 )   246 - 247   1995.6

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  • The Role of Sugar Moieties of Elicitor and Suppressor in Plant-Fungal Parasite Recognition : (2) Effect of Monosaccharides on the Accumulation of Pisatin

    YOSHIMOTO Y., TOYODA K., KATO T., ICHINOSE Y., YAMADA T., SHIRAISHI T.

    Annals of the Phytopathological Society of Japan   61 ( 3 )   247 - 247   1995.6

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  • Characterization of Concanavalin A-Binding Protein Complex from Pea Plasma Membranes

    TOYODA K., MIKI K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

    Annals of the Phytopathological Society of Japan   61 ( 3 )   247 - 247   1995.6

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  • RELATIONSHIP BETWEEN DEFENSE RESPONSESAND ION FLUX

    AMANO Masashi, TOYODA Kazuhiro, ICHINOSE Yuki, YAMADA Tetsuji, SHIRAISHI Tomonori

    36   S119   1995.3

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  • Mechanism on Recognition of Elicitor : Lectins Stimulate Phytoalexin Production

    MIKI K, TOYODA K, ICHINOSE Y, YAMADA T, SHIRAISHI T

    Annals of the Phytopathological Society of Japan   60 ( 6 )   767 - 767   1994.12

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  • Effect of Orthovanadate in Pea Suspension-Cultured Cells

    KAWAHARA T, TOYODA K, ISHINOSE Y, YAMADA T, SHIRAISHI T

    Annals of the Phytopathological Society of Japan   60 ( 6 )   766 - 766   1994.12

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  • Relationship between Defense Responses and Ion Flux : (I). Effect of Several Ionophores and Ionchannel Blockers on the Accumulation of Pisatin

    AMANO M, TOYODA K, YAMADA T, ICHINOSE Y, YAMADA T, SHIRAISHI T

    Annals of the Phytopathological Society of Japan   60 ( 6 )   766 - 767   1994.12

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  • SUPPRESSORS - DETERMINANTS OF SPECIFICITY PRODUCED BY PLANT-PATHOGENS

    T SHIRAISHI, T YAMADA, K SAITOH, T KATO, K TOYODA, H YOSHIOKA, HM KIM, Y ICHINOSE, M TAHARA, H OKU

    PLANT AND CELL PHYSIOLOGY   35 ( 8 )   1107 - 1119   1994.12

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    Plant pathogens secrete suppressors that delay or prevent the host defense responses, with resultant conditioning of host cells such that they become susceptible even to avirulent or nonpathogenic microorganisms. Suppressors have been characterized as glycoproteins, glycopeptides, peptides and anionic and nonanionic glucans. A suppressor itself is non-toxic to plant cells and, thus, it can be distinguished from host-specific toxins produced by certain pathogens. Suppressors disturb fundamental functions of host plasma membranes. For example, the suppressor from a pea pathogen, Mycosphaerella pinodes, inhibits both the ATPase activity and polyphosphoinositide metabolism in pea plasma membranes, causing the temporary suppression of the signal-transduction pathway that leads to the expression of defense genes, which encode key enzymes in the biosynthetic pathway to phytoalexin. In this review, evidence for the role of suppressors in the determination of plant host-parasite specificity is summarized.

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  • Specific Inhibition of ATPase Tightly Binding to Cell Wall (Cell Wall ATPase) by Fungal Suppressor-Fractionation of Cell Wall ATPase by Triton X-100 PAGE

    KIBA A., TOYODA K., YAMADA T., ICHINOSE Y., SHIRAISHI T.

    Annals of the Phytopathological Society of Japan   60 ( 3 )   315 - 315   1994.6

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  • Regulation of Defense Responses in Pea by Fungal Signals, Elicitor and Suppressor from Mycosphaerella pinodes. : (8) In vitro Production of Second Messenger-like Molecule (s)

    KOYAMA M., MIZUKOSHI R., YOSHIOKA H., TOYODA K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

    Annals of the Phytopathological Society of Japan   60 ( 3 )   315 - 315   1994.6

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  • Regulation of Defense Responses in Pea by Fungal Signals from Mycosphaerella pinodes : (VII).Association of Plasma Membrane ATPase with Several Enzymes Involved in Polyphosphoinositide Metabolism

    TOYODA K., MIKI K., ICHINOSE Y., YAMADA T., SHIRAISHI T.

    Annals of the Phytopathological Society of Japan   59 ( 6 )   752 - 753   1993.12

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  • Effect of the Suppressor from a Pea Pathogen,Mycosphaerella pinodes,on H^+-pumping Activity of Reconstituted Pea Plasma Membrane ATPase

    AMANO M., TOYODA K., HINO I., ICHINOSE Y., YAMADA T., SHIRAISHI T.

    Annals of the Phytopathological Society of Japan   59 ( 6 )   752 - 752   1993.12

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  • Regulation of Defense Responses in Pea bY Fungal Signals froM MycoSPHAERELLA pinodes : (VI). Possibility of Cross-Talk between Plasma Membrane ATPase and Polyphosphoinositide Metabolism

    HINO I., TOYODA K., KATO T., SHIRAISHI T., YAMADA T., OKU H., ICHINOSE Y.

    Annals of the Phytopathological Society of Japan   59 ( 3 )   268 - 269   1993.6

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  • Specific Action of Suppressor from Mycosphaerella pinodes on the ATPase Activity in Cell Wall Fraction from Pea Plants

    KIBA A., YAMAGUCHI Y., TOYODA K., KATO T., SHIRAISHI T., YAMADA T., OKU H.

    Annals of the Phytopathological Society of Japan   59 ( 3 )   268 - 268   1993.6

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  • Inhibitory Effects of Suppressors from Mycosphaerella pinodes and Their Peptide Moieties on the Pea Plasma Membrane ATPase Activity

    KATO T., TOYODA K., SAITO K., TAHARA M., SHIRAISHI T., YAMADA T., OKU H

    Annals of the Phytopathological Society of Japan   59 ( 1 )   85 - 85   1993.2

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  • Regulation of Defense Response Expression in Pea by Fungal Signals, Elicitor and Suppressor, from a Pea Pathogen,Mycosphaerella pinodes.Effects of Elicitor and/or Suppressor on Phospholipase C Activity in Plasma Membranes

    TOYODA K., KATO T., SHIRAISHI T., YAMADA T., OKU H., ICHINOSE Y.

    Annals of the Phytopathological Society of Japan   59 ( 1 )   85 - 85   1993.2

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  • A water-soluble extract from germlings of Erysiphe pisi suppresses infection by E. graminis in coleoptiles cells of barley(共著)

    Kazuhiro TOYODA, Tomonori SHIRAISHI, Issei KOBAYASHI, Naoto YAMAOKA, Hitoshi KUNOH

    Annals of the Phytopathological Society of Japan   59 ( 2 )   135 - 142   1993

  • Expression of Defense Responses in Pea Regulated by Two Signals from a Pathogenic Fungus : (3) Relationship between ATPase Activity and Lipid Metabolism in Isolated Pea Plasma Membranes

    TOYODA K., HINO I., KATO T., SHIRAISHI T., ICHINOSE Y., YAMADA T., OKU H.

    Annals of the Phytopathological Society of Japan   58 ( 4 )   569 - 570   1992.10

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  • In situ Inhibition of ATPase Activity in Pea Plasma Membranes by Suppressors from a Pea Pathogen,Mycosphaerella pinodes

    OKUDA J., YAMAGUCHI Y., KIM H., TOYODA K., KATO T., SHIRAISHI T., YAMADA T., ICHINOSE Y., OKU H.

    Annals of the Phytopathological Society of Japan   58 ( 4 )   570 - 570   1992.10

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  • (9) 病原菌によるエンドウ病害抵抗性の制御機構 (1) : ポリホスホイノシチド代謝系の制御 (関西部会)

    豊田 和弘, 吉岡 博文, 白石 友紀, 一瀬 勇規, 山田 哲治, 奥 八郎

    日本植物病理學會報   58 ( 1 )   126 - 126   1992.1

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  • (90) オオムギ子葉鞘細胞の認識機構 : (26) Erysiphe pisi の拒否性インデューサーによる一細胞処理と拒否性誘導との関係 (日本植物病理学会大会)

    豊田 和弘, 加藤 信也, 白石 友紀, 小林 一成, 山岡 直人, 久能 均

    日本植物病理學會報   57 ( 3 )   416 - 416   1991.7

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  • (84) オオムギ子葉鞘細胞の認識機構 (XX) 拒否性誘導因子の諸性質 (1) (関西部会)

    豊田 和弘, 白石 友紀, 小林 一成, 山岡 直人, 久能 均

    日本植物病理學會報   57 ( 1 )   113 - 113   1991.1

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  • (12) オオムギ子葉鞘細胞の認識機構 : (XV) 非病原菌Erysiphe pisiからのオオムギに対する拒否性誘導因子の検索 (関西部会講演要旨)

    豊田 和弘, 小林 一成, 山岡 直人, 久能 均, 白石 友紀

    日本植物病理學會報   56 ( 1 )   119 - 119   1990.1

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Presentations

  • CEP14 ペプチドによるシロイヌナズナの免疫応答の制御

    石田壮太, 伊藤千晶, 長谷川晴香, 澤田健太郎, 松井英譲, 能年義輝, 一瀬勇規, 白石友紀, 豊田和弘

    令和5年度日本植物病理学会関西部会  2023.9.24 

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  • シロイヌナズナにおける CEP ペプチドの成長と免疫に対する作用

    長谷川晴香, 伊藤千晶, 澤田健太郎, 石田壮太, 松井英譲, 能年義輝, 一瀬勇規, 白石友紀, 豊田和弘

    令和5年度日本植物病理学会関西部会  2023.9.24 

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  • 非親和性および不適応型病原細菌を接種したシロイヌナズナ葉における CEP 遺伝子の応答

    Aprilia Nur Fitrianti, 伊藤千晶, 長谷川晴香, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    令和4年度日本植物病理学会大会  2022.3.27 

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  • 病原体感染およびサリチル酸に応答したシロイヌナズナにおける CEP 遺伝子の誘導

    伊藤千晶, 長谷川晴香, Aprilia Nur Fitrianti, 松井英譲, 山本幹博, 能年義輝, 一瀬勇規, 白石友紀, 豊田和弘

    令和4年度日本植物病理学会関西部会  2022.9.22 

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    Event date: 2022.9.21 - 2022.9.22

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  • 保存され分泌されるペプチドのファミリーである CEP ペプチドはシロイヌナズナの免疫 を負に調節する(4) CEP ペプチドはサリチル酸を介した免疫を抑制する

    長谷川晴香, 伊藤千晶, Aprilia Nur Fitrianti, 松井英譲, 山本幹博, 能年義輝, 一, 瀬勇規, 白石友紀, 豊田和弘

    植物微生物研究会第31回研究交流会  2022.9.9 

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    Event date: 2022.9.8 - 2022.9.10

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  • 保存され分泌されるペプチドのファミリーである CEP ペプチドはシロイヌナズナの免疫を負に調節する(3) CEP ペプチドは PTI および ETI を抑制する

    伊藤千晶, 長谷川晴香, Aprilia Nur Fitrianti, 松井英譲, 山本幹博, 能年義輝, 一瀬勇規, 白石友紀, 豊田和弘

    植物微生物研究会第31回研究交流会  2022.9.9 

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  • CEP peptides, a family of conserved, secreted small peptides act as endogenous suppressors in Arabidopsis Invited

    Kazuhiro Toyoda

    12th US-Japan Seminar in Plant Pathology  2022.9.29 

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  • CEP peptides, a family of conserved, secreted peptides that negatively modulate Arabidopsis immunity I. Potential role during PTI and ETI

    Chiaki Itoh, Haruka Hasegawa, Aprilia Nur Fitrianti, Hidenori Matsui, Mikihiro Yamamoto, Yoshiteru Noutoshi, Yuki Ichinose, Tomonori Shiraishi, Kazuhiro Toyoda

    2022.8.29 

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  • CEP peptides, a family of conserved, secreted peptides that negatively modulate Arabidopsis immunity II. CEP peptide attenuates SA-mediated immunity

    Haruka Hasegawa, Chiaki Itoh, Aprilia Nur Fitrianti, Hidenori Matsui, Mikihiro Yamamoto, Yoshiteru Noutoshi, Yuki Ichinose, Tomonori Shiraishi, Kazuhiro Toyoda

    12th US-Japan Seminar in Plant Pathology  2022.8.29 

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  • CEP peptide suppresses innate immunity and promotes disease development in Arabidopsis thaliana

    Aprilia Nur Fitrianti, Chiaki Itoh, Haruka Hasegawa, Hidenori Matsui, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi, Kazuhiro Toyoda

    2021.9.21 

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    Event date: 2021.9.21 - 2021.9.22

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  • シロイヌナズナの MAMP 誘導性免疫における細胞壁ペルオキシダーゼと NADPH オキシダーゼの関 与

    木元菜々子, 高須瑞穂, Aprilia Nur Fitrianti, 松井英讓, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    令和3年度日本植物病理学会関西部会  2021.9.21 

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    Event date: 2021.9.21 - 2021.9.22

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  • メトミノストロビンの抵抗性誘導作用に関する研究 7.シロイヌナズナにおける PTI および SAR 関連 遺伝子の発現に対する影響

    伊藤千晶, 小原七海, 佐藤穂高, 市成光広, 山田 晶, 櫻本和生, 白石 慎, 山本 隆, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    令和3年度日本植物病理学会関西部会  2021.9.21 

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    Event date: 2021.9.21 - 2021.9.22

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  • シロイヌナズナにおける MAMP 誘導性 ROS バーストにおけるペルオキシダーゼの寄与

    木元菜々子, 高須瑞穂, Aprilia Nur Fitrianti, 松井英讓, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    令和 3 年度(第 5 5 回)植物感染生理談話会  2021.9.2 

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    Event date: 2021.9.1 - 2021.9.3

    Language:Japanese   Presentation type:Poster presentation  

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  • メトミノストロビンの抵抗性誘導作用に関する研究 9.シロイヌナズナにおける PTI および SAR 関連遺伝子の発現に対する影響

    伊藤千晶, 小原七海, 佐藤穂高, 市成光広, 山田 晶, 櫻本和生, 白石 慎, 山本 隆, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    令和 3 年度(第 5 5 回)植物感染生理談話会  2021.9.2 

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    Event date: 2021.9.1 - 2021.9.3

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  • 植物免疫を調節する病原体サプレッサーと内生サプレッサー Invited

    豊田和弘

    令和 3 年度(第 5 5 回)植物感染生理談話会  2021.9.2 

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    Event date: 2021.9.1 - 2021.9.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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  • メトミノストロビンの抵抗性誘導作用に関する研究 6.シロイヌナズナにおける PTI 関連遺伝子の発現に対する 影響

    小原七海, 伊藤千晶, 佐藤穂高, 市成光広, 山田 晶, 櫻本和生, 白石 慎, 山本 隆, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    令和3年度日本植物病理学会大会  2021.3.17 

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    Event date: 2021.3.17 - 2021.3.19

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  • CEP5 peptide negatively modulates defense responses in Arabidopsis thaliana

    N.F. Aprilia, M.T. Luan, H. Matsui, Y. Noutoshi, M. Yamamoto, Y. Ichinose, T. Shiraishi, K. Toyoda

    2021.3.17 

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    Event date: 2021.3.17 - 2021.3.19

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  • メトミノストロビンの抵抗性誘導作用に関する研究 5.シロイヌナズナのPTI 応答ににおける MAP キナーゼ活 性に対する影響

    伊藤千晶, 小原七海, 佐藤穂高, 市成光広, 山田 晶, 櫻本和生, 白石 慎, 山本 隆, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    令和3年度日本植物病理学会大会  2021.3.17 

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    Event date: 2021.3.17 - 2021.3.19

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  • メトミノストロビンの抵抗性誘導作用に関する研究(4) メトミノストロビンはシロイヌ ナズナにおいて PTI 関連遺伝子の発現を促進する

    小原七海, 伊藤千晶, 佐藤穂高, 市成光広, 山田 晶, 櫻本和生, 白石 慎, 山本 隆, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和弘

    日本農薬学会第46回大会  2021.3.9 

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    Event date: 2021.3.8 - 2021.3.10

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  • メトミノストロビンの抵抗性誘導作用に関する研究 (3) メトミノストロビンはシロイヌ ナズナの MAP キナーゼを活性化する

    伊藤千晶, 小原七海, 佐藤穂高, 市成光広, 山田 晶, 櫻本和生, 白石 慎, 山本 隆, 松井英譲, 能年義輝, 山本幹博, 一瀬勇規, 白石友紀, 豊田和, 弘

    日本農薬学会第46回大会  2021.3.9 

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    Event date: 2021.3.8 - 2021.3.10

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Awards

  • 財団法人農学会日本農学進歩賞

    2004  

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    Country:Japan

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  • 第1回日韓植物バイオテクノロジー合同シンポジウム最優秀ポスター賞

    1999  

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  • 日本植物病理学会 学術奨励賞

    1999  

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    Country:Japan

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Research Projects

  • New regulatory mechanism on plant immunity via CEP peptide

    Grant number:21K05597  2021.04 - 2024.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    豊田 和弘

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    Grant amount:\4160000 ( Direct expense: \3200000 、 Indirect expense:\960000 )

    植物の病原体に対する免疫応答の活性化についての研究は盛んに行われてきたが、免疫を負に調節する仕組みについては世界的にも殆ど解析されていない。このような状況の中、シロイヌナズナの健全な植物体から免疫抑制因子(内生サプレッサー)の探索と精製を進め、その1つが C-terminally encoded peptide 5 (CEP5)のフラグメントであることをすでに明らかにしている。実際、化学合成した CEP5 ペプチドのフラグメントまたは成熟型ペプチドで前処理した植物体では不適応型菌や非親和静菌による感染が成立する。当該年度は、免疫応答時における CEP ペプチドの役割について明らかにする目的で、CEP5 遺伝子を含むすべての CEP 遺伝子 (CEP1-CEP15) の病原体や植物ホルモンに対する応答について調べた。その結果、強い免疫応答を伴う病原性欠損株、非親和性菌ならびに不適応型菌の接種に応答して複数の CEP 遺伝子の発現が誘導されたが、親和性菌では CEP14 遺伝子を除いて明確な応答は認められなかった。すなわち、CEP 遺伝子は ETI や PTI に伴って誘導されること、さらに親和性の組み合わせでも一部の CEP 遺伝子が拡大抵抗性に伴って発現が誘導されるものと推測された。事実、CEP 遺伝子はサリチル酸の処理によって発現が増加することが明らかとなった。このことは、CEP 遺伝子の発現が植物ホルモンによる調節を受けていることを示唆している。以上の結果は、遺伝子応答に伴って生成する一群の CEP ペプチドが「過剰な免疫応答を未然に調節し、成長と防御の最適化を図る」という考え(仮説)を強く支持している。今後は、病原菌やホルモン応答性のすべての CEP ペプチド(成熟型)を化学合成し、これらの免疫応答に対する作用について調べるとともに、CEP 遺伝子の過剰発現体や抑制個体を用いた解析を進め、この仮説の妥当性について検証していく。

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  • Dynamism on pathogen recognition and subsequent responses through the plant cell wall

    Grant number:18K05645  2018.04 - 2021.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    TOYODA Kazuhiro

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    Grant amount:\4420000 ( Direct expense: \3400000 、 Indirect expense:\1020000 )

    In recent years, research on plant immunity (PTI) through pattern recognition receptors (PRRs) has progressed, but its relationship (interaction) with signal transduction originating from the plant cell wall remains unknown. We have previously shown that the plant cell wall directly recognizes pathogens through constituent(s) and initiates intracellular and extracellular defense responses. We also made efforts on a molecular mechanism by which pathogen- and plant-derived suppressors attenuate elicitor/MAMP-induced defense responses. In this study, we found a role of the plant cell wall during the PTI response, and demonstrated the existence of an unknown signaling pathway that does not require known molecules.

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  • 基本的親和性の破綻を利用した病害抵抗性植物の作出

    Grant number:18658019  2006 - 2008

    日本学術振興会  科学研究費助成事業  萌芽研究

    豊田 和弘

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    Grant amount:\3200000 ( Direct expense: \3200000 )

    本研究課題では、病原糸状菌との基本的親和性成立に関与する植物遺伝子について解析し、その機能の改変に基づいた新たな病害防除技術を提案することを目的としている。これまでに、エンドウ褐紋病菌が生産する病原性因子(サプレッサー)に対する宿主植物の応答遺伝子をサプレッション・サブトラクティブハイブリダイゼーション(SSH)法で解析した。シーケンスの結果、約150遺伝子が単離され、その1つに葉緑体局在型のリポキシゲナーゼ(LOX,lipoxygenase)をコードする遺伝子が含まれることが明らかとなった。一般に、葉緑体局在のLOXはジャスモン酸合成への関与が推定され、サプレッサーが宿主植物のジャスモン酸合成経路を転写レベルから活性化していることが予想された。実際、LOXとその下流で働くallene oxide synthase(AOX)、allene oxide cyclase(AOC)ならびに12-oxophytodienoic acid reductase(OPR)のサイレンシング個体では、エンドウ褐紋病菌に対する罹病性が低下し、病斑形成の抑制が認められた。この結果は、サプレッサーの作用発現にはLOXを介した応答(ジャスモン酸経路)が関与し、これと拮抗するサリチル酸経路に依存した防御応答の抑制が関連しているものと考えられた。同様に、毒性発現にジャスモン酸経路を必要とする植物細菌毒素(コロナチン)の作用を調べた結果、いずれのサイレンシング植物でも壊死斑の形成が著しく阻害(遅延)された。以上の結果は、サプレッサーを含む病原性因子の作用発現は宿主の情報伝達系や代謝に依存しており、それらに関与する宿主遺伝子の改変によって、一部の病原菌(糸状菌、細菌)に対する耐性を付与できることを示す。

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  • Mechanism in recognition of non-self sustained by the interaction between organelles in plant cell

    Grant number:15108001  2003 - 2007

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (S)

    SHIRAISHI Tomonori, ICHINOSE Yuki, INAGAKI Yoshishige, TOYODA Kazuhiro

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    Grant amount:\109720000 ( Direct expense: \84400000 、 Indirect expense:\25320000 )

    In this research, we attempted to clarify the plant defense mechanism in recognition of MAMPs, signal transmission and action of virulence effectors, mediated by the interaction between respective organelles. In this fiscal year, we showed that several molecules interacted with apyrase in plant cell wall by PAGE and TOF/MS/MS analyses. We also presented that MAMPs activated the cell-wall-bound apyrase, resulting in increasing inorganic phosphate that stimulated generation of active oxygen species and expression of defense-related genes. MAMPs also regulated the expression of NbCdc27B, which is a component of M phase in cell cycle. NbCdc27B participated in defense reaction without hypersensitive cell death Microarray analysis indicated that the transcriptional activation of WRKY41 was induced by MAMPs. Transformants highly expressed WRKY41 showed resistance to Pseudomonas syringae, but, inversely, susceptiblity to Erwinia carotovara, indicating that WRKY41 participated in SA signaling pathway. Transportation of proteins via endoplasmic reticulum may be also crucial to defense. Based on these findings, we discuss defense signaling sustained by respective organelles.

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  • Analysis of the transcriptional activators for plant defense response-related genes

    Grant number:12460023  2000 - 2002

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    ICHINOSE Yuki, TOYODA Kazuhiro

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    Grant amount:\13200000 ( Direct expense: \13200000 )

    In this study, we investigated the structure and function of novel transcription factor E84 (ERDP) that is isolated from elicitor-treated pea epicotyls. E84 is a plant specific transcription factor possessing Dof-type DNA binding domain at the N-terminus. Random site selection assay for E84 revealed that the recombinant E84 protein binds to AAAG-containing DNA fragments. DNA binding assay using a model DNA fragment, BS4/37 (TCATTTAAAGTGTTTT) that could bind to E84, revealed that not only AAAG core sequence but also 3'-TGT sequence were required for high affinity of the binding. E84 protein also bound to the DNA fragments in the promoter of the elicitor-responsive gene, PsCHS1, which contain AAAG sequence. When we carried out transient transfection assay with pea protoplasts and reporter plasmid possessing 4 repeats of "BS4/37"/CaMV 35S minimal promoter plus reporter gene, we observed that the elicitor enhanced reporter gene activity, indicating the possible involvement of AAAG sequence in elicitor-mediated transcriptional activation. We also observed that E84 protein binds to the promoter sequence of E84 gene itself, indicating possible mechanism of auto-regulation. These results show that E84 protein and its binding sequences might contribute to the elicitor-mediated transcriptional regulation. Furthermore, we also found that at least 7 members of the Dof gene family in pea. These observations provide the information to elucidate the mechanism of the transcriptional activation for plant defense-response related genes.

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  • 植物細胞における病原菌認識装置の分離とそれらの再構成に関する基礎的研究

    Grant number:11760036  1999 - 2000

    日本学術振興会  科学研究費助成事業  奨励研究(A)

    豊田 和弘

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    Grant amount:\2200000 ( Direct expense: \2200000 )

    植物-病原菌の相互作用における宿主特異性決定機構の解明は、自他識別という生命現象の根幹に迫りうる課題であり、幾多の応用的価値を秘めている。植物の感染病をモデルとすれば、感染の成立(罹病化)、または拒絶(抵抗化)が特異性決定の指標となる。これまでに、エンドウー褐紋病菌Mycosphaerella pinodesをモデルとして、病原菌に対する認識は先ず宿主の細胞壁で行われ、下流の原形質膜シグナル伝達系が制御されていることが次第に明らかとなってきた。そこで、本研究では、細胞壁における病原菌認識と、これに引き続く情報伝達の流れを明らかにすることを目的とした。すなわち、エンドウ細胞壁ならびに原形質膜可溶化タンパク質から病原菌シグナル物質(サプレッサー、エリシター)に対して応答する分子(酵素活性)を探索し、エンドウcDNAライブラリーからそれらのクローニングを行った。一方、大腸菌で発現させた組み換えタンパク質を抗原としてポリクローナル抗体を作製するとともに、ビオチン標識、またはそれらを固相化したアフィニティーゲル担体を作製し、シグナル伝達系における相互作用分子の探索を進めた。この結果、原形質膜シグナル伝達系を構成するリピッドキナーゼ(組み換えタンパク質)を直接リン酸化する原形質膜タンパク質の存在が明らかとなった。また、分離原形質膜におけるリピッドキナーゼはエリシターで活性化し、逆にサプレッサーやタンパク質リン酸化酵素阻害剤の存在下には著しく抑制されたことから、本リン酸化酵素は原形質膜から細胞内部への情報伝達に深く関与しているものと推定された。現在、本酵素の上流で働くタンパク質の精製・解析を進めてる。

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  • 病原菌の宿主特異性とその分子機構

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  • Molecular mechanism on host-parasite specificity

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  • Advances in Plant Science (2023academic year) Prophase  - 火5~6,金5~6

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  • Plant Disease and Control (2020academic year) Third semester  - 木5,木6

  • Course Seminar 3 (2020academic year) 1st and 2nd semester  - その他

  • Course Seminar 4 (2020academic year) 3rd and 4th semester  - その他

  • Molecular Plant Pathology (2020academic year) Late  - 火7,火8

  • Undergraduate's-level thesis research (2020academic year) 1st-4th semester  - その他

  • Mechanisms in Plant Infection (2020academic year) Prophase  - その他

  • Seminar in Plant Pathology (2020academic year) Prophase  - その他

  • Seminar in Plant Pathology (2020academic year) Late  - その他

  • Seminar in Plant Pathology (2020academic year) Late  - その他

  • Seminar in Plant Pathology (2020academic year) Prophase  - その他

  • Plant Pathology 1 (2020academic year) 1st semester  - 木3,木4

  • Plant Pathology 2 (2020academic year) Second semester  - 木3,木4

  • Specific Research of Science for Bio-Production (2020academic year) Year-round  - その他

  • Seminars in Special Field of Study 1 (2020academic year) 1st and 2nd semester  - その他

  • Seminars in Special Field of Study 2 (2020academic year) 3rd and 4th semester  - その他

  • Plant Disease and Control (2020academic year) Third semester  - 木5,木6

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