Updated on 2025/06/10

写真a

 
木羽　隆敏
 
Organization
Scheduled update Professor
Position
Professor
External link

Research Interests

  • サイトカイニン

  • 窒素

  • 植物ホルモン

  • 植物栄養

Education

  • Nagoya University   大学院生命農学研究科   生物機構・機能科学専攻

    2000.4 - 2003.3

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  • Nagoya University   生命農学研究科   生物機構・機能科学専攻

    1998.4 - 2000.3

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    Notes： 博士課程前期課程修了

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  • Nagoya University   農学部   応用生命科学科

    1994.4 - 1998.3

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Research History

  • The University of Tokyo   生産工学研究センター

    2018.4 - 2021.3

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  • 理化学研究所   客員主幹研究員

    2018.4 - 2021.3

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  • Nagoya University   Associate Professor

    2018.3

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  • The University of Tokyo   Biotechnology Research Center

    2017.4 - 2018.3

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  • RIKEN   イノベーション推進センター・ 植物新育種技術研究チーム

    2016.4 - 2018.3

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  • RIKEN   CSRS

    2014.4 - 2018.3

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  • RIKEN   CSRS

    2013.4 - 2014.3

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  • Rothamsted Research

    2010.8 - 2010.12

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  • RIKEN   PSC

    2008.10 - 2013.3

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  • The Rockefeller University   Laboratory of Plant Molecular Biology (Nam-Hai Chua lab)

    2004.8 - 2008.10

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  • Nagoya University   Graduate School of Bioagricultural Sciences

    2003.4 - 2004.7

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  • Nagoya University   Graduate School of Bioagricultural Sciences

    2000.4 - 2003.3

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Papers

  • The cytokinin efflux transporter ABCC4 participates in Arabidopsis root system development

    Takuya Uragami, Takatoshi Kiba, Mikiko Kojima, Yumiko Takebayashi, Yuzuru Tozawa, Yuki Hayashi, Toshinori Kinoshita, Hitoshi Sakakibara

    Plant Physiology   2024.12

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/plphys/kiae628

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  • A florigen-expressing subpopulation of companion cells expresses other small proteins and reveals a nitrogen-sensitiveFTrepressor

    Hiroshi Takagi, Shogo Ito, Jae Sung Shim, Akane Kubota, Andrew K. Hempton, Nayoung Lee, Takamasa Suzuki, Chansie Yang, Christine T. Nolan, Kerry L. Bubb, Cristina M. Alexandre, Daisuke Kurihara, Yoshikatsu Sato, Yasuomi Tada, Takatoshi Kiba, Jose L. Pruneda-Paz, Christine Queitsch, Josh T. Cuperus, Takato Imaizumi

    2024.8

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    Publisher：Cold Spring Harbor Laboratory  

    Abstract

    The precise onset of flowering is crucial to ensure successful plant reproduction. The geneFLOWERING LOCUS T(FT) encodes florigen, a mobile signal produced in leaves that initiates flowering at the shoot apical meristem. In response to seasonal changes,FTis induced in phloem companion cells located in distal leaf regions. Thus far, a detailed molecular characterization of theFT-expressing cells has been lacking. Here, we used bulk nuclei RNA-seq and single nuclei RNA (snRNA)-seq to investigate gene expression inFT-expressing cells and other phloem companion cells. Our bulk nuclei RNA-seq demonstrated thatFT-expressing cells in cotyledons and in true leaves differed transcriptionally. Within the true leaves, our snRNA-seq analysis revealed that companion cells with highFTexpression form a unique cluster in which many genes involved in ATP biosynthesis are highly upregulated. The cluster also expresses other genes encoding small proteins, including the flowering and stem growth inducer FPF1-LIKE PROTEIN 1 (FLP1) and the anti-florigen BROTHER OF FT AND TFL1 (BFT). In addition, we found that the promoters ofFTand the genes co-expressed withFTin the cluster were enriched for the consensus binding motifs of NITRATE-INDUCIBLE GARP-TYPE TRANSCRIPTIONAL REPRESSOR 1 (NIGT1). Overexpression of the paralogousNIGT1.2andNIGT1.4repressedFTexpression and significantly delayed flowering under nitrogen-rich conditions, consistent with NIGT1s acting as nitrogen-dependentFTrepressors. Taken together, our results demonstrate that majorFT-expressing cells show a distinct expression profile that suggests that these cells may produce multiple systemic signals to regulate plant growth and development.

    DOI： 10.1101/2024.08.17.608367

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  • The endophytic fungus Serendipita indica affects auxin distribution in Arabidopsis thaliana roots through alteration of auxin transport and conjugation to promote plant growth. International journal

    Adrián González Ortega-Villaizán, Eoghan King, Manish K Patel, Marta-Marina Pérez-Alonso, Sandra S Scholz, Hitoshi Sakakibara, Takatoshi Kiba, Mikiko Kojima, Yumiko Takebayashi, Patricio Ramos, Luis Morales-Quintana, Sarah Breitenbach, Ana Smolko, Branka Salopek-Sondi, Nataša Bauer, Jutta Ludwig-Müller, Anne Krapp, Ralf Oelmüller, Jesús Vicente-Carbajosa, Stephan Pollmann

    Plant, cell & environment   2024.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    Plants share their habitats with a multitude of different microbes. This close vicinity promoted the evolution of interorganismic interactions between plants and many different microorganisms that provide mutual growth benefits both to the plant and the microbial partner. The symbiosis of Arabidopsis thaliana with the beneficial root colonizing endophyte Serendipita indica represents a well-studied system. Colonization of Arabidopsis roots with S. indica promotes plant growth and stress tolerance of the host plant. However, until now, the molecular mechanism by which S. indica reprograms plant growth remains largely unknown. This study used comprehensive transcriptomics, metabolomics, reverse genetics, and life cell imaging to reveal the intricacies of auxin-related processes that affect root growth in the symbiosis between A. thaliana and S. indica. Our experiments revealed the sustained stimulation of auxin signalling in fungus infected Arabidopsis roots and disclosed the essential role of tightly controlled auxin conjugation in the plant-fungus interaction. It particularly highlighted the importance of two GRETCHEN HAGEN 3 (GH3) genes, GH3.5 and GH3.17, for the fungus infection-triggered stimulation of biomass production, thus broadening our knowledge about the function of GH3s in plants. Furthermore, we provide evidence for the transcriptional alteration of the PIN2 auxin transporter gene in roots of Arabidopsis seedlings infected with S. indica and demonstrate that this transcriptional adjustment affects auxin signalling in roots, which results in increased plant growth.

    DOI： 10.1111/pce.14989

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  • DNA- and Selectable-Marker-Free Genome-Editing System Using Zygotes from Recalcitrant Maize Inbred B73.

    Hajime Yamada, Norio Kato, Masako Ichikawa, Keiko Mannen, Takatoshi Kiba, Yuriko Osakabe, Hitoshi Sakakibara, Minami Matsui, Takashi Okamoto

    Plant & cell physiology   65 ( 5 )   729 - 736   2024.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    Genome-editing tools such as the clustered regularly interspaced short palindromic repeats/Cas9 (CRISPR/Cas9) system have become essential tools for increasing the efficiency and accuracy of plant breeding. Using such genome-editing tools on maize, one of the most important cereal crops of the world, will greatly benefit the agriculture and the mankind. Conventional genome-editing methods typically used for maize involve insertion of a Cas9-guide RNA expression cassette and a selectable marker in the genome DNA; however, using such methods, it is essential to eliminate the inserted DNA cassettes to avoid legislative concerns on gene-modified organisms. Another major hurdle for establishing an efficient and broadly applicable DNA-free genome-editing system for maize is presented by recalcitrant genotypes/cultivars, since cell/tissue culture and its subsequent regeneration into plantlets are crucial for producing transgenic and/or genome-edited maize. In this study, to establish a DNA-free genome-editing system for recalcitrant maize genotypes/cultivars, Cas9-gRNA ribonucleoproteins were directly delivered into zygotes isolated from the pollinated flowers of the maize-B73 cultivar. The zygotes successfully developed and were regenerated into genome-edited plantlets by co-culture with phytosulfokine, a peptide phytohormone. The method developed herein made it possible to obtain DNA- and selectable-marker-free genome-edited recalcitrant maize genotypes/cultivars with high efficiency. This method can advance the molecular breeding of maize and other important cereals, regardless of their recalcitrant characteristics.

    DOI： 10.1093/pcp/pcae010

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  • ArabidopsisABCC4encodes a cytokinin efflux transporter and is involved in root system development

    Takuya Uragami, Takatoshi Kiba, Mikiko Kojima, Yumiko Takebayashi, Yuzuru Tozawa, Yuki Hayashi, Toshinori Kinoshita, Hitoshi Sakakibara

    2024.5

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  • The Root-Colonizing Endophyte Piriformospora indica Supports Nitrogen-Starved Arabidopsis thaliana Seedlings with Nitrogen Metabolites. International journal

    Sandra S Scholz, Emanuel Barth, Gilles Clément, Anne Marmagne, Jutta Ludwig-Müller, Hitoshi Sakakibara, Takatoshi Kiba, Jesús Vicente-Carbajosa, Stephan Pollmann, Anne Krapp, Ralf Oelmüller

    International journal of molecular sciences   24 ( 20 )   2023.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    The root-colonizing endophytic fungus Piriformospora indica promotes the root and shoot growth of its host plants. We show that the growth promotion of Arabidopsis thaliana leaves is abolished when the seedlings are grown on media with nitrogen (N) limitation. The fungus neither stimulated the total N content nor did it promote 15NO3- uptake from agar plates to the leaves of the host under N-sufficient or N-limiting conditions. However, when the roots were co-cultivated with 15N-labelled P. indica, more labels were detected in the leaves of N-starved host plants but not in plants supplied with sufficient N. Amino acid and primary metabolite profiles, as well as the expression analyses of N metabolite transporter genes suggest that the fungus alleviates the adaptation of its host from the N limitation condition. P. indica alters the expression of transporter genes, which participate in the relocation of NO3-, NH4+ and N metabolites from the roots to the leaves under N limitation. We propose that P. indica participates in the plant's metabolomic adaptation against N limitation by delivering reduced N metabolites to the host, thus alleviating metabolic N starvation responses and reprogramming the expression of N metabolism-related genes.

    DOI： 10.3390/ijms242015372

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  • The trans-zeatin-type side-chain modification of cytokinins controls rice growth. International journal

    Takatoshi Kiba, Kahori Mizutani, Aimi Nakahara, Yumiko Takebayashi, Mikiko Kojima, Tokunori Hobo, Yuriko Osakabe, Keishi Osakabe, Hitoshi Sakakibara

    Plant physiology   192 ( 3 )   2457 - 2474   2023.7

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    Cytokinins (CKs), a class of phytohormones with vital roles in growth and development, occur naturally with various side-chain structures, including N6-(Δ2-isopentenyl)adenine-, cis-zeatin- and trans-zeatin (tZ)-types. Recent studies in the model dicot plant Arabidopsis (Arabidopsis thaliana) have demonstrated that tZ-type CKs are biosynthesized via cytochrome P450 monooxygenase (P450) CYP735A and have a specific function in shoot growth promotion. Although the function of some of these CKs has been demonstrated in a few dicotyledonous plant species, the importance of these variations and their biosynthetic mechanism and function in monocots and in plants with distinctive side-chain profiles other than Arabidopsis, such as rice (Oryza sativa), remain elusive. In this study, we characterized CYP735A3 and CYP735A4 to investigate the role of tZ-type CKs in rice. Complementation test of the Arabidopsis CYP735A-deficient mutant and CK profiling of loss-of-function rice mutant cyp735a3 cyp735a4 demonstrated that CYP735A3 and CYP735A4 encode P450s required for tZ-type side-chain modification in rice. CYP735As are expressed in both roots and shoots. The cyp735a3 cyp735a4 mutants exhibited growth retardation concomitant with reduction in CK activity in both roots and shoots, indicating that tZ-type CKs function in growth promotion of both organs. Expression analysis revealed that tZ-type CK biosynthesis is negatively regulated by auxin, abscisic acid, and CK and positively by dual nitrogen nutrient signals, namely glutamine-related and nitrate-specific signals. These results suggest that tZ-type CKs control the growth of both roots and shoots in response to internal and environmental cues in rice.

    DOI： 10.1093/plphys/kiad197

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  • Photosynthetic-Product-Dependent Activation of Plasma Membrane H+-ATPase and Nitrate Uptake in Arabidopsis Leaves.

    Satoru N Kinoshita, Takamasa Suzuki, Takatoshi Kiba, Hitoshi Sakakibara, Toshinori Kinoshita

    Plant & cell physiology   64 ( 2 )   191 - 203   2023.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    Plasma membrane (PM) proton-translocating adenosine triphosphatase (H+-ATPase) is a pivotal enzyme for plant growth and development that acts as a primary transporter and is activated by phosphorylation of the penultimate residue, threonine, at the C-terminus. Small Auxin-Up RNA family proteins maintain the phosphorylation level via inhibiting dephosphorylation of the residue by protein phosphatase 2C-D clade. Photosynthetically active radiation activates PM H+-ATPase via phosphorylation in mesophyll cells of Arabidopsis thaliana, and phosphorylation of PM H+-ATPase depends on photosynthesis and photosynthesis-related sugar supplementation, such as sucrose, fructose and glucose. However, the molecular mechanism and physiological role of photosynthesis-dependent PM H+-ATPase activation are still unknown. Analysis using sugar analogs, such as palatinose, turanose and 2-deoxy glucose, revealed that sucrose metabolites and products of glycolysis such as pyruvate induce phosphorylation of PM H+-ATPase. Transcriptome analysis showed that the novel isoform of the Small Auxin-Up RNA genes, SAUR30, is upregulated in a light- and sucrose-dependent manner. Time-course analyses of sucrose supplementation showed that the phosphorylation level of PM H+-ATPase increased within 10 min, but the expression level of SAUR30 increased later than 10 min. The results suggest that two temporal regulations may participate in the regulation of PM H+-ATPase. Interestingly, a 15NO3- uptake assay in leaves showed that light increases 15NO3- uptake and that increment of 15NO3- uptake depends on PM H+-ATPase activity. The results opened the possibility of the physiological role of photosynthesis-dependent PM H+-ATPase activation in the uptake of NO3-. We speculate that PM H+-ATPase may connect photosynthesis and nitrogen metabolism in leaves.

    DOI： 10.1093/pcp/pcac157

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  • The calcium sensor CBL7 is required for Serendipita indica-induced growth stimulation in Arabidopsis thaliana, controlling defense against the endophyte and K+ homoeostasis in the symbiosis

    Marta-Marina Perez-Alonso, Carmen Guerrero-Galan, Adrian Gonzalez Ortega-Villaizan, Paloma Ortiz-Garcia, Sandra S. Scholz, Patricio Ramos, Hitoshi Sakakibara, Takatoshi Kiba, Jutta Ludwig-Mueller, Anne Krapp, Ralf Oelmueller, Jesus Vicente-Carbajosa, Stephan Pollmann

    PLANT CELL AND ENVIRONMENT   45 ( 11 )   3367 - 3382   2022.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/pce.14420

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  • ABCG11 modulates cytokinin responses in Arabidopsis thaliana

    Qianying Yang, Jie Zhang, Mikiko Kojima, Yumiko Takebayashi, Takuya Uragami, Takatoshi Kiba, Hitoshi Sakakibara, Youngsook Lee

    FRONTIERS IN PLANT SCIENCE   13   2022.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3389/fpls.2022.976267

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  • Isolation of gametes and zygotes from Setaria viridis

    Erika Toda, Takatoshi Kiba, Norio Kato, Takashi Okamoto

    JOURNAL OF PLANT RESEARCH   135 ( 4 )   627 - 633   2022.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s10265-022-01393-w

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  • Excessive ammonium assimilation by plastidic glutamine synthetase causes ammonium toxicity in Arabidopsis thaliana. International journal

    Takushi Hachiya, Jun Inaba, Mayumi Wakazaki, Mayuko Sato, Kiminori Toyooka, Atsuko Miyagi, Maki Kawai-Yamada, Daisuke Sugiura, Tsuyoshi Nakagawa, Takatoshi Kiba, Alain Gojon, Hitoshi Sakakibara

    Nature communications   12 ( 1 )   4944 - 4944   2021.8

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    Plants use nitrate, ammonium, and organic nitrogen in the soil as nitrogen sources. Since the elevated CO2 environment predicted for the near future will reduce nitrate utilization by C3 species, ammonium is attracting great interest. However, abundant ammonium nutrition impairs growth, i.e., ammonium toxicity, the primary cause of which remains to be determined. Here, we show that ammonium assimilation by GLUTAMINE SYNTHETASE 2 (GLN2) localized in the plastid rather than ammonium accumulation is a primary cause for toxicity, which challenges the textbook knowledge. With exposure to toxic levels of ammonium, the shoot GLN2 reaction produced an abundance of protons within cells, thereby elevating shoot acidity and stimulating expression of acidic stress-responsive genes. Application of an alkaline ammonia solution to the ammonium medium efficiently alleviated the ammonium toxicity with a concomitant reduction in shoot acidity. Consequently, we conclude that a primary cause of ammonium toxicity is acidic stress.

    DOI： 10.1038/s41467-021-25238-7

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  • Low nitrogen conditions accelerate flowering by modulating the phosphorylation state of FLOWERING BHLH 4 in Arabidopsis. International journal

    Miho Sanagi, Shoki Aoyama, Akio Kubo, Yu Lu, Yasutake Sato, Shogo Ito, Mitsutomo Abe, Nobutaka Mitsuda, Masaru Ohme-Takagi, Takatoshi Kiba, Hirofumi Nakagami, Filip Rolland, Junji Yamaguchi, Takato Imaizumi, Takeo Sato

    Proceedings of the National Academy of Sciences of the United States of America   118 ( 19 )   2021.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1073/pnas.2022942118

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  • Mechanism of nitrogen transport in plants Invited Reviewed

    Japanese Journal of Soil Science and Plant Nutrition   92   76 - 91   2021

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  • NIGT1 family proteins exhibit dual mode DNA recognition to regulate nutrient response-associated genes in Arabidopsis

    Yoshiaki Ueda, Li-Jia Qu, Shohei Nosaki, Yasuhito Sakuraba, Takuya Miyakawa, Takatoshi Kiba, Masaru Tanokura, Shuichi Yanagisawa

    PLOS Genetics   16 ( 11 )   e1009197 - e1009197   2020.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1371/journal.pgen.1009197

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  • Nitrogen and Phosphorus interactions in plants: from agronomic to physiological and molecular insights

    Gabriel Krouk, Takatoshi Kiba

    Current Opinion in Plant Biology   57   104 - 109   2020.10

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    Publishing type：Research paper (scientific journal)   Publisher：Elsevier {BV}  

    DOI： 10.1016/j.pbi.2020.07.002

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  • A genome resource for green millet Setaria viridis enables discovery of agronomically valuable loci Reviewed International journal

    Mamidi, S, Healey, A, Huang, P, Grimwood, J, Jenkins, J, Barry, K, Sreedasyam, A, Shu, S, Lovell, J.T, Feldman, M, Wu, J, Yu, Y, Chen, C, Johnson, J, Sakakibara, H, Kiba, T, Sakurai, T, Tavares, R, Nusinow, D.A, Baxter, I, Schmutz, J, Brutnell, T.P, Kellogg, E.A

    Nature Biotech.   38 ( 10 )   1203 - 1210   2020.10

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    Wild and weedy relatives of domesticated crops harbor genetic variants that can advance agricultural biotechnology. Here we provide a genome resource for the wild plant green millet (Setaria viridis), a model species for studies of C4 grasses, and use the resource to probe domestication genes in the close crop relative foxtail millet (Setaria italica). We produced a platinum-quality genome assembly of S. viridis and de novo assemblies for 598 wild accessions and exploited these assemblies to identify loci underlying three traits: response to climate, a 'loss of shattering' trait that permits mechanical harvest and leaf angle, a predictor of yield in many grass crops. With CRISPR-Cas9 genome editing, we validated Less Shattering1 (SvLes1) as a gene whose product controls seed shattering. In S. italica, this gene was rendered nonfunctional by a retrotransposon insertion in the domesticated loss-of-shattering allele SiLes1-TE (transposable element). This resource will enhance the utility of S. viridis for dissection of complex traits and biotechnological improvement of panicoid crops.

    DOI： 10.1038/s41587-020-0681-2

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  • Harnessing symbiotic plant–fungus interactions to unleash hidden forces from extreme plant ecosystems

    Marta-Marina Pérez-Alonso, Carmen Guerrero-Galán, Sandra S Scholz, Takatoshi Kiba, Hitoshi Sakakibara, Jutta Ludwig-Müller, Anne Krapp, Ralf Oelmüller, Jesús Vicente-Carbajosa, Stephan Pollmann, Peter Doerner

    Journal of Experimental Botany   71 ( 13 )   3865 - 3877   2020.6

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    DOI： 10.1093/jxb/eraa040

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  • Nitrate-inducible NIGT1 proteins modulate phosphate uptake and starvation signalling via transcriptional regulation of SPX genes. International journal

    Yoshiaki Ueda, Takatoshi Kiba, Shuichi Yanagisawa

    The Plant journal : for cell and molecular biology   102 ( 3 )   448 - 466   2020.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/tpj.14637

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  • Flowering time control in rice by introducing Arabidopsis clock-associated PSEUDO-RESPONSE REGULATOR 5

    Norihito Nakamichi, Toru Kudo, Nobue Makita, Takatoshi Kiba, Toshinori Kinoshita, Hitoshi Sakakibara

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   84 ( 5 )   970 - 979   2020.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1080/09168451.2020.1719822

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  • Genome Sequence of Striga asiatica Provides Insight into the Evolution of Plant Parasitism Reviewed International journal

    Yoshida, S, Kim, S, Wafula, E. K, Tanskanen, J, Kim, Y. M, Honaas, L, Yang, Z, Spallek, T, Conn, C. E, Ichihashi, Y, Cheong, K, Cui, S, Der, J. P, Gundlach, H, Jiao, Y, Hori, C, Ishida, J. K, Kasahara, H, Kiba, T, Kim, M. S, Koo, N, Laohavisit, A, Lee, Y. H, Lumba, S, McCourt, P, Mortimer, J. C, Mutuku, J. M, Nomura, T, Sekimoto, Y. S, Seto, Y, Wang, Y, Wakatake, T, Sakakibara, H, Demura, T, Yamaguchi, S, Yoneyama, K, Manabe, R, Nelson, D. C, Schulman, A. H, Timko, M. P, dePamphilis, C. W, Choi, D, Shirasu, K

    Current Biology   29 ( 18 )   3041 - 3052   2019.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.cub.2019.07.086

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  • Sugar-induced de novo cytokinin biosynthesis contributes to Arabidopsis growth under elevated CO2 Reviewed

    Takatoshi Kiba, Yumiko Takebayashi, Mikiko Kojima, Hitoshi Sakakibara

    Scientific Reports   9 ( 1 )   2019.5

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s41598-019-44185-4

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  • An efficient DNA- and selectable-marker-free genome-editing system using zygotes in rice Reviewed International journal

    Erika Toda, Narumi Koiso, Arika Takebayashi, Masako Ichikawa, Takatoshi Kiba, Keishi Osakabe, Yuriko Osakabe, Hitoshi Sakakibara, Norio Kato, Takashi Okamoto

    Nature Plants   5 ( 4 )   363 - 368   2019.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s41477-019-0386-z

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  • Shoot nitrate underlies a perception of nitrogen satiety to trigger local and systemic signaling cascades in Arabidopsis thaliana Reviewed

    Okamoto Yuki, Suzuki Takamasa, Sugiura Daisuke, Kiba Takatoshi, Sakakibara Hitoshi, Hachiya Takushi

    SOIL SCIENCE AND PLANT NUTRITION   65 ( 1 )   56 - 64   2019.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1080/00380768.2018.1537643

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  • Chromatin-mediated feed-forward auxin biosynthesis in floral meristem determinacy Reviewed International journal

    Nobutoshi Yamaguchi, Jiangbo Huang, Yoshitaka Tatsumi, Masato Abe, Shigeo S. Sugano, Mikiko Kojima, Yumiko Takebayashi, Takatoshi Kiba, Ryusuke Yokoyama, Kazuhiko Nishitani, Hitoshi Sakakibara, Toshiro Ito

    Nature Communications   9 ( 1 )   5290 - 5290   2018.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s41467-018-07763-0

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  • International Symposium on Long-Distance Signaling in Plants Reviewed

    Kiba, T.

    Plant and Cell Physiology   59 ( 9 )   1697 - 1699   2018.9

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/pcp/pcy151

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  • Genome editing in rice by direct delivery of preassembled CRISPR-Cas9 vectors or ribonucleoproteins into zygotes

    Takashi Okamoto, Erika Toda, Narumi Koiso, Arika Takebayashi, Masako Ichikawa, Takatoshi Kiba, Keishi Osakabe, Yuriko Osakabe, Hitoshi Sakakibara, Norio Kato

    IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT   54   S88 - S89   2018.8

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  • SUPERMAN regulates floral whorl boundaries through control of auxin biosynthesis Reviewed

    Yifeng Xu, Nathanaël Prunet, Eng-Seng Gan, Yanbin Wang, Darragh Stewart, Frank Wellmer, Jiangbo Huang, Nobutoshi Yamaguchi, Yoshitaka Tatsumi, Mikiko Kojima, Takatoshi Kiba, Hitoshi Sakakibara, Thomas P Jack, Elliot M Meyerowitz, Toshiro Ito

    EMBO Journal   37 ( 11 )   2018.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.15252/embj.201797499

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  • Repression of Nitrogen Starvation Responses by Members of the Arabidopsis GARP-Type Transcription Factor NIGT1/HRS1 Subfamily Reviewed

    Takatoshi Kiba, Jun Inaba, Toru Kudo, Nanae Ueda, Mineko Konishi, Nobutaka Mitsuda, Yuko Takiguchi, Youichi Kondou, Takeshi Yoshizumi, Masaru Ohme-Takagi, Minami Matsui, Kentaro Yano, Shuichi Yanagisawa, Hitoshi Sakakibara

    The Plant Cell   30 ( 4 )   925 - 945   2018.4

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    DOI： 10.1105/tpc.17.00810

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  • A NIGT1-centred transcriptional cascade regulates nitrate signalling and incorporates phosphorus starvation signals in Arabidopsis Reviewed

    Maeda, Y, Konishi, M, Kiba, T, Sakuraba, Y, Sawaki, N, Kurai, T, Ueda, Y, Sakakibara, H, Yanagisawa, S

    Nature Communications   9 ( 1 )   2018

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s41467-018-03832-6

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  • Systemic transport of trans-zeatin and its precursor have differing roles in Arabidopsis shoots Reviewed

    Asami Osugi, Mikiko Kojima, Yumiko Takebayashi, Nanae Ueda, Takatoshi Kiba, Hitoshi Sakakibara

    Nature Plants   3 ( 8 )   2017.7

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    DOI： 10.1038/nplants.2017.112

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  • Interspecies hormonal control of host root morphology by parasitic plants Reviewed

    Spallek, Thomas, Melnyk, Charles W., Wakatake, Takanori, Zhang, Jing, Sakamoto, Yuki, Kiba, Takatoshi, Yoshida, Satoko, Matsunaga, Sachihiro, Sakakibara, Hitoshi, Shirasu, Ken

    Proceedings of the National Academy of Sciences of the United States of America   114 ( 20 )   5283 - 5288   2017

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    DOI： 10.1073/pnas.1619078114

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  • Systemic transport of trans-zeatin and its precursor have differing roles in Arabidopsis shoots Reviewed

    Osugi, Asami, Kojima, Mikiko, Takebayashi, Yumiko, Ueda, Nanae, Kiba, Takatoshi, Sakakibara, Hitoshi

    Nature Plants   3 ( 8 )   2017

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    DOI： 10.1038/nplants.2017.112

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  • Kinetics of the LOV domain of ZEITLUPE determine its circadian function in Arabidopsis Reviewed

    Pudasaini, Ashutosh, Shim, Jae Sung, Song, Young Hun, Shi, Hua, Kiba, Takatoshi, Somers, David E., Imaizumi, Takato, Zoltowski, Brian D.

    Elife   6   2017

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    DOI： 10.7554/eLife.21646

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  • Improvement of Arabidopsis Biomass and Cold, Drought and Salinity Stress Tolerance by Modified Circadian Clock-Associated PSEUDO-RESPONSE REGULATORs Reviewed

    Nakamichi, Norihito, Takao, Saori, Kudo, Toru, Kiba, Takatoshi, Wang, Yin, Kinoshita, Toshinori, Sakakibara, Hitoshi

    Plant and Cell Physiology   57 ( 5 )   1085 - 1097   2016

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    DOI： 10.1093/pcp/pcw057

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  • Plant Nitrogen Acquisition Under Low Availability: Regulation of Uptake and Root Architecture Reviewed

    Kiba, Takatoshi, Krapp, Anne

    Plant and Cell Physiology   57 ( 4 )   707 - 714   2016

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    DOI： 10.1093/pcp/pcw052

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  • Assessing protein stability under different light and circadian conditions

    Kiba, T., Henriques, R.

    Methods in Molecular Biology   1398   2016

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    DOI： 10.1007/978-1-4939-3356-3_13

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  • Biosynthesis and transport of cytokinins

    Kiba Takatoshi, Sakakibara Hitoshi

    Regulation of Plant Growth & Development   51 ( 1 )   24 - 29   2016

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    <p>Plants maximize fitness by coordinating growth and development of cells as well as organs in response to environmental changes. For the coordination, cell-to-cell and long-distance communication among cells and organs is necessary. Cytokinins, a class of phytohormones that play key roles in various processes of plant growth and development, have been implicated in the communication. It has been shown that cytokinin action is regulated at various steps, including de novo biosynthesis, degradation, transport, perception, and signaling, to play roles in the communication. This review summarizes and provides updates on the regulatory mechanisms of cytokinin action with major emphasis on cytokinin de novo biosynthesis and transport. We also introduce the mechanisms of cytokinin biosynthesis by phytopathogens that exploit cytokinin action for their infection.</p>

    DOI： 10.18978/jscrp.51.1_24

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  • The Arabidopsis nitrate transporter NRT2.5 plays a role in nitrate acquisition and remobilization in nitrogen-starved plants Reviewed

    Lezhneva, Lina, Kiba, Takatoshi, Feria-Bourrellier, Ana-Belen, Lafouge, Florence, Boutet-Mercey, Stephanie, Zoufan, Parzhak, Sakakibara, Hitoshi, Daniel-Vedele, Francoise, Krapp, Anne

    Plant Journal   80 ( 2 )   230 - 241   2014

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    DOI： 10.1111/tpj.12626

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  • Effects of High CO2 on Growth and Metabolism of Arabidopsis Seedlings During Growth with a Constantly Limited Supply of Nitrogen Reviewed

    Takatani, Nobuyuki, Ito, Takuro, Kiba, Takatoshi, Mori, Marie, Miyamoto, Tetsuro, Maeda, Shin-ichi, Omata, Tatsuo

    Plant and Cell Physiology   55 ( 2 )   281 - 292   2014

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    DOI： 10.1093/pcp/pct186

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  • Arabidopsis ABCG14 is essential for the root-to-shoot translocation of cytokinin Reviewed

    Ko, Donghwi, Kang, Joohyun, Kiba, Takatoshi, Park, Jiyoung, Kojima, Mikiko, Do, Jihye, Kim, Kyung Yoon, Kwon, Mi, Endler, Anne, Song, Won-Yong, Martinoia, Enrico, Sakakibara, Hitoshi, Lee, Youngsook

    Proceedings of the National Academy of Sciences of the United States of America   111 ( 19 )   7150 - 7155   2014

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    DOI： 10.1073/pnas.1321519111

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  • Side-Chain Modification of Cytokinins Controls Shoot Growth in Arabidopsis Reviewed

    Kiba, Takatoshi, Takei, Kentaro, Kojima, Mikiko, Sakakibara, Hitoshi

    Developmental Cell   27 ( 4 )   452 - 461   2013

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    DOI： 10.1016/j.devce1.2013.10.004

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  • Arabidopsis lonely guy (LOG) multiple mutants reveal a central role of the LOG-dependent pathway in cytokinin activation Reviewed

    Tokunaga, Hiroki, Kojima, Mikiko, Kuroha, Takeshi, Ishida, Takashi, Sugimoto, Keiko, Kiba, Takatoshi, Sakakibara, Hitoshi

    Plant Journal   69 ( 2 )   355 - 365   2012

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    DOI： 10.1111/j.1365-313X.2011.04795.x

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  • The response and recovery of the Arabidopsis thaliana transcriptome to phosphate starvation

    Woo, Jongchan, MacPherson, Cameron Ross, Liu, Jun, Wang, Huan, Kiba, Takatoshi, Hannah, Matthew A., Wang, Xiu-Jie, Bajic, Vladimir B., Chua, Nam-Hai

    Bmc Plant Biology   12   2012

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    DOI： 10.1186/1471-2229-12-62

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  • The Arabidopsis Nitrate Transporter NRT2.4 Plays a Double Role in Roots and Shoots of Nitrogen-Straved Plants Reviewed

    Kiba, Takatoshi, Feria-Bourrellier, Ana-Belen, Lafouge, Florence, Lezhneva, Lina, Boutet-Mercey, Stephanie, Orsel, Mathilde, Brehaut, Virginie, Miller, Anthony, Daniel-Vedele, Francoise, Sakakibara, Hitoshi, Krapp, Anne

    Plant Cell   24 ( 1 )   245 - 258   2012

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    DOI： 10.1105/tpc.111.092221

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  • Transcriptional repressor PRR5 directly regulates clock-output pathways Reviewed

    Nakamichi, Norihito, Kiba, Takatoshi, Kamioka, Mari, Suzuki, Takamasa, Yamashino, Takafumi, Higashiyama, Tetsuya, Sakakibara, Hitoshi, Mizuno, Takeshi

    Proceedings of the National Academy of Sciences of the United States of America   109 ( 42 )   17123 - 17128   2012

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    DOI： 10.1073/pnas.1205156109

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  • Deep sequencing of small RNAs specifically associated with Arabidopsis AGO1 and AGO4 uncovers new AGO functions Reviewed

    Wang, Huan, Zhang, Xiuren, Liu, Jun, Kiba, Takatoshi, Woo, Jongchan, Ojo, Tolulope, Hafner, Markus, Tuschl, Thomas, Chua, Nam-Hai, Wang, Xiu-Jie

    Plant Journal   67 ( 2 )   292 - 304   2011

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    DOI： 10.1111/j.1365-313X.2011.04594.x

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  • Hormonal control of nitrogen acquisition: roles of auxin, abscisic acid, and cytokinin Reviewed

    Kiba, T, Kudo, T, Kojima, M, Sakakibara, H

    Journal of Experimental Botany   62 ( 4 )   1399 - 1409   2011

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    DOI： 10.1093/jxb/erq410

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  • Role of cytokinin in the regulation of plant development Reviewed

    T. Kiba, H. Sakakibara

    Plant Developmental Biology   2   237 - 254   2010

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    DOI： 10.1007/978-3-642-04670-4_13

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  • PSEUDO-RESPONSE REGULATORS 9, 7, and 5 Are Transcriptional Repressors in the Arabidopsis Circadian Clock Reviewed

    Nakamichi, N, Kiba, T, Henriques, R, Mizuno, T, Chua, NH, Sakakibara, H

    Plant Cell   22 ( 3 )   594 - 605   2010

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    DOI： 10.1105/tpc.109.072892

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  • F-Box Proteins FKF1 and LKP2 Act in Concert with ZEITLUPE to Control Arabidopsis Clock Progression Reviewed

    Baudry, A, Ito, S, Song, YH, Strait, AA, Kiba, T, Lu, S, Henriques, R, Pruneda-Paz, JL, Chua, NH, Tobin, EM, Kay, SA, Imaizumi, T

    Plant Cell   22 ( 3 )   606 - 622   2010

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    DOI： 10.1105/tpc.109.072843

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  • Metabolism and Long-distance Translocation of Cytokinins Reviewed

    Kudo, T, Kiba, T, Sakakibara, H

    Journal of Integrative Plant Biology   52 ( 1 )   53 - 60   2010

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    DOI： 10.1111/j.1744-7909.2010.00898.x

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  • Rhythmic and light-inducible appearance of clock-associated pseudo-response regulator protein PRR9 through programmed degradation in the dark in Arabidopsis thaliana (vol 48, pg 1644, 2007) Reviewed

    Shogo Ito, Norihito Nakamichi, Takatoshi Kiba, Takafumi Yamashino, Takeshi Mizuno

    PLANT AND CELL PHYSIOLOGY   49 ( 1 )   133 - 133   2008.1

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  • A Small Subfamily of Arabidopsis RADIALIS-LIKE SANT/MYB Genes: A Link to HOOKLESS1-Mediated Signal Transduction during Early Morphogenesis Reviewed

    Hamaguchi, A, Yamashino, T, Koizumi, N, Kiba, T, Kojima, M, Sakakibara, H, Mizuno, T

    Bioscience Biotechnology and Biochemistry   72 ( 10 )   2687 - 2696   2008

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    DOI： 10.1271/bbb.80348

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  • The AtGenExpress hormone and chemical treatment data set: experimental design, data evaluation, model data analysis and data access Reviewed

    Goda, H, Sasaki, E, Akiyama, K, Maruyama-Nakashita, A, Nakabayashi, K, Li, WQ, Ogawa, M, Yamauchi, Y, Preston, J, Aoki, K, Kiba, T, Takatsuto, S, Fujioka, S, Asami, T, Nakano, T, Kato, H, Mizuno, T, Sakakibara, H, Yamaguchi, S, Nambara, E, Kamiya, Y, Takahashi, H, Hirai, MY, Sakurai, T, Shinozaki, K, Saito, K, Yoshida, S, Shimada, Y

    Plant Journal   55 ( 3 )   526 - 542   2008

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    DOI： 10.1111/j.1365-313X.2008.03510.x

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  • The occurrence of clock-associated and light-induced PRR9 pseudo-response regulator protein in Arabidopsis plants is diurnally oscillated and subjected to programmed-degradation in the dark Reviewed

    Shogo Ito, Norihito Nakamichi, Takatoshi Kiba, Akinori Matsushika, Toru Fujimori, Takafami Yamashino, Takeshi Mizuno

    PLANT AND CELL PHYSIOLOGY   48   S151 - S151   2007

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  • ZEITLUPE targets PSEUDO-RESPONSE REGULATOR 5 (PRR5) to 26S proteasome-dependent degradation Reviewed

    Kiba Takatoshi, Henriques Rossana, Sakakibara Hitoshi, Chua Nam-Hai

    PLANT AND CELL PHYSIOLOGY   48   S187   2007

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  • A link between cytokinin and ASL9 (ASYMMETRIC LEAVES 2 LIKE 9) that belongs to the AS2/LOB (LATERAL ORGAN BOUNDARIES) family genes in Arabidopsis thaliana Reviewed

    Naito, T, Yamashino, T, Kiba, T, Koizumi, N, Kojima, M, Sakakibara, H, Mizuno, T

    Bioscience Biotechnology and Biochemistry   71 ( 5 )   1269 - 1278   2007

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    DOI： 10.1271/bbb.60681

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  • Characterization of a unique GATA family gene that responds to both light and cytokinin in Arabidopsis thaliana Reviewed

    Naito, T, Kiba, T, Koizumi, N, Yamashino, T, Mizuno, T

    Bioscience Biotechnology and Biochemistry   71 ( 6 )   1557 - 1560   2007

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    DOI： 10.1271/bbb.60692

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  • Targeted degradation of PSEUDO-RESPONSE REGULATOR5 by an SCFZTL complex regulates clock function and photomorphogenesis in Arabidopsis thaliana Reviewed

    Kiba, T, Henriques, R, Sakakibara, H, Chua, NH

    Plant Cell   19 ( 8 )   2516 - 2530   2007

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    DOI： 10.1105/tpc.107.053033

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  • Rhythmic and light-inducible appearance of clock-associated pseudo-response regulator protein PRR9 through programmed degradation in the dark in Arabidopsis thaliana Reviewed

    Ito, S, Nakamichi, N, Kiba, T, Yamashino, T, Mizuno, T

    Plant and Cell Physiology   48 ( 11 )   1644 - 1651   2007

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    DOI： 10.1093/pcp/pcm122

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  • The Arabidopsis SPA1 gene is required for circadian clock function and photoperiodic flowering

    Ishikawa, M., Kiba, T., Chua, NH

    Plant Journal   46 ( 5 )   2006

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    DOI： 10.1111/j.1365-313X.2006.02737.x

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  • Combinatorial microarray analysis revealing Arabidopsis genes implicated in cytokinin responses through the His -&gt; Asp phosphorelay circuitry Reviewed

    Kiba, T, Naitou, T, Koizumi, N, Yamashino, T, Sakakibara, H, Mizuno, T

    Plant and Cell Physiology   46 ( 2 )   339 - 355   2005

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    DOI： 10.1093/pcp/pci033

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  • Rapid response of Arabidopsis T87 cultured cells to cytokinin through His-to-Asp phosphorelay signal transduction Reviewed

    Yamada, H, Koizumi, N, Nakamichi, N, Kiba, T, Yamashino, T, Mizuno, T

    Bioscience Biotechnology and Biochemistry   68 ( 9 )   1966 - 1976   2004

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    DOI： 10.1271/bbb.68.1966

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  • Arabidopsis response regulator, ARR22, ectopic expression of which results in phenotypes similar to the wol cytokinin-receptor mutant Reviewed

    Kiba, T, Aoki, K, Sakakibara, H, Mizuno, T

    Plant and Cell Physiology   45 ( 8 )   1063 - 1077   2004

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    DOI： 10.1093/pcp/pch128

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  • Comparative studies on the type-B response regulators revealing their distinctive properties in the His-to-Asp phosphorelay signal transduction of Arabidopsis thaliana Reviewed

    Tajima, Y, Imamura, A, Kiba, T, Amano, Y, Yamashino, T, Mizuno, T

    Plant and Cell Physiology   45 ( 1 )   28 - 39   2004

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    DOI： 10.1093/pcp/pcg154

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  • The type-A response regulator, ARR15, acts as a negative regulator in the cytokinin-mediated signal transduction in Arabidopsis thaliana

    Kiba, T., Yamada, H., Sato, S., Kato, T., Tabata, S., Yamashino, T., Mizuno, T.

    Plant and Cell Physiology   44 ( 8 )   868 - 874   2003

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    DOI： 10.1093/pcp/pcg108

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  • Hormonal regulation of plant metabolism: cytokinin action and signal transduction

    Kiba, T., Mizuno, T.

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   48 ( 15 Suppl )   2003

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  • In vivo and in vitro characterization of the ARR11 response regulator implicated in the His-to-Asp phosphorelay signal transduction in Arabidopsis thaliana Reviewed

    Imamura, A, Kiba, T, Tajima, Y, Yamashino, T, Mizuno, T

    Plant and Cell Physiology   44 ( 2 )   122 - 131   2003

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    DOI： 10.1093/pcp/pcg014

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  • Characterization of the ARR15 and ARR16 response regulators with special reference to the cytokinin signaling pathway mediated by the AHK4 histidine kinase in roots of Arabidopsis thaliana Reviewed

    Kiba, T, Yamada, H, Mizuno, T

    Plant and Cell Physiology   43 ( 9 )   1059 - 1066   2002

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    DOI： 10.1093/pcp/pcf121

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  • APRR1/TOC1 family: Insight into Arabidopsis circadian clock Reviewed

    Mizuno T, Matsushika A, Makino S, Kojima M, Kiba T, Imamura A, Hanaki N, Nakamura A, Suzuki T, Taniguchi M, Ueguchi C, Sugiyama T

    Plant and Cell Physiology   43   S22   2002

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  • Compilation and characterization of Arabidopsis thaliana response regulators implicated in His-Asp phosphorelay signal transduction

    Imamura, A, Hanaki, N, Nakamura, A, Suzuki, T, Taniguchi, M, Kiba, T, Ueguchi, C, Sugiyama, T, Mizuno, T

    Plant and Cell Physiology   40 ( 7 )   733 - 742   1999

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    DOI： 10.1093/oxfordjournals.pcp.a029600

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  • Expression of Arabidopsis response regulator homologs is induced by cytokinins and nitrate

    Taniguchi, M., Kiba, T., Sakakibara, H., Ueguchi, C., Mizuno, T., Sugiyama, T.

    Febs Letters   429 ( 3 )   1998

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    DOI： 10.1016/S0014-5793(98)00611-5

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MISC

  • Genes Encoding Pseudo-Response Regulators : Insight into His-to Asp Phosphorelay and Circadian Rhythm in Arabidopsis thaliana :

    Makino Seiya, Kiba Takatoshi, Imamura Aya, Hanaki Naoto, Nakamura Ayako, Suzuki Tomomi, Taniguchi Mitsutaka, Ueguchi Chiharu, Sugiyama Tatsuo, Mizuno Takeshi

    Plant and cell physiology   41 ( 6 )   791 - 803   2000

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    Language：English   Publisher：Japanese Society of Plant Physiologists  

    In the higher plant, Arabidopsis thaliana, results from recent intensive studies suggested that His-to-Asp phosphorelay mechanisms are involved presumably in propagation of environmental stimuli, such as phytohormones(e.g.ethylene and cytokinin). Here we identified and characterized a set of novel Arabidopsis genes whose products considerably resemble the authentic response regulators(ARR-series)of Arabidopsis in the sense that they have a phospho-accepting receiver-like domain. However, they should be discriminated from the classical ones in the strict sense that they lack the invariant phospho-accepting aspartate site. They were thus named APRRs(Arabidopsis pseudo-response regulators). Two such representatives, APRR1 and APRR2, were characterized extensively through cloning of the corresponding cDNAs, in terms of their structural designs, biochemical properties, subcellular localization in plant cells, and expression profiles at the transcriptional level. The result of in vitro phosphorylation experiment with the Arabidopsis AHP phosphotransmitter suggested that the pseudo-receivers have no ability to undergo phosphorylation. The result of transient expression assay with onion epidermal cells showed that the GFP-APRR1 fusion protein has an ability to enter into the nuclei. The C-terminal domain of APRR1, termed CONSTANS-motif, appears to be responsible for the nuclear-localization. The most intriguing result was that the accumulation of APRR1 transcript is subjected to a circadian rhythm. The APRR1 protein is identical to the one that was recently suggested to interact with the ABI3(ABISCISIC ACID INSENSITIVE3)protein. These are discussed with special reference to the His-to-Asp phosphorelay signal transduction and circadian rhythm in Arabidopsis thaliana.

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  • Differential Expression of Genes for Response Regulators in Response to Cytokinins and Nitrate in Arabidopsis thaliana :

    Kiba Takatoshi, Taniguchi Mitsutaka, Imamura Aya, Ueguchi Chiharu, Mizuno Takeshi, Sugiyama Tatsuo

    Plant and cell physiology   40 ( 7 )   767 - 771   1999

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    In Arabidopsis thaliana, a number of response regulators are presumably involved in His-Asp phosphorelay signal transduction in response to environmental stimuli, such as phytohormones. Previously, it was shown that expression of a certain set of genes for response regulators are cytokinin- and nitrate-responsive in their mRNA accumulation, under certain growth conditions[Taniguchi et al.(1998)FEBS Lett.429: 259, Brandstatter and Kieber (1998)Plant Cell 10: 1009]. To answer the critical question of whether or not other response regulator genes, so far identified in Arabidopsis thaliana, are also cytokinin-inducible, here an extended comparative examination was carried out. It was demonstrated that not all of response regulator genes are necessarily cytokinin-responsive in their transcription. Rather, the members of a certain subfamily(type-A) are cytokinin-responsive, but those belonging to the other(type-B) are not. The presumed nitrate-responsiveness was also assessed for the same set of response regulators, and the analogous view was supported. These results suggest that the two subtypes of response regulators differ from each other, as judged from not only their structural designs, but also the expression profiles of their transcripts in response to plant stimuli.

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Research Projects

  • Nitrogen starvation response in a C4 model plant, Setaria viridis

    Grant number：20K21271  2020.07 - 2022.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)  Grant-in-Aid for Challenging Research (Exploratory)

    Kiba Takatoshi

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    Grant amount：\6370000 （ Direct expense: \4900000 、 Indirect expense：\1470000 ）

    The molecular mechanisms of how plants sense and respond to nitrogen deficiency, which they often encounter in the natural environment, are not well understood. One reason for this is that the forward genetic approach using Arabidopsis is approaching its limits. In this study, we attempted to solve this problem by developing a new forward genetic research material, Setaria viridis, a C4 monocotyledonous plant. To this end, we produced a platinum-quality genome assembly and de novo assemblies for 598 wild accessions, created a mutant library, determined the cultivation conditions that induce nitrogen deficiency in juvenile and mature plants, and collected basic data on the physiological response of the plant to nitrogen deficiency.

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  • 低投入持続型農業に適した作物品種開発のための基盤研究

    2020.07 - 2021.06

    三島海雲記念財団  ２０２０年度学術研究奨励金（個人研究奨励金） 

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    Authorship：Principal investigator 

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  • 植物における窒素飢餓応答の分子基盤

    Grant number：20H02888  2020.04 - 2024.03

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    木羽 隆敏

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    Grant amount：\17290000 （ Direct expense: \13300000 、 Indirect expense：\3990000 ）

    本年度は研究実施計画に従い、以下の①、②の２項目について研究を進めた。
    ①窒素飢餓応答制御に関わる新奇因子の探索：申請者ら独自の窒素飢餓マーカーを指標に(a)変異体スクリーニング、(b)ケミカルスクリーニング、(c)Yeast one-hybridスクリーニングにより新奇因子の探索を行った。
    (a)窒素飢餓レポーターライン（pNRT2.4:GUSレポーターライン）にEMSで変異を導入したM2集団（10,000系統のM1由来）を確立し、窒素充足条件下にも関わらず窒素飢餓レポーターを発現する変異体のスクリーニング条件の確立と１次スクリーニングを行った。複数ラインの変異体候補を得た。(b) ケミカルスクリーニングを行うための栽培条件を確立し、理研-ITbMの標準ライブラリーについて、窒素充足条件下にも関わらず窒素飢餓レポーター（pNRT2.4:GFP）を発現させる化合物のスクリーニングを行った。ヒット化合物候補が複数得られた。(c) Yeast one-hybridスクリーニングのライブラリーとレポーター株の作製を行った。
    <BR>
    ②新奇・既知因子の役割と因子間の遺伝的関係の明確化によるシステム理解：窒素飢餓応答に関わるとされる既知因子の変異体及び過剰発現体の確立を進めた。NIGT1s、LBD37-39、CBL7、 BTB1/2については、変異体を確立した。NIGT1sとLBD37-39については過剰発現体を確立した。CEPDsとNLAについては、Col-0 背景の変異体と過剰発現体作製を開始した。

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  • Molecular mechanisms and physiological roles of long-distance cytokinin transport

    Grant number：18H04793  2018.04 - 2020.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    木羽 隆敏

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    Grant amount：\10140000 （ Direct expense: \7800000 、 Indirect expense：\2340000 ）

    サイトカイニン（CK）は植物の成長・発達の様々な過程の制御に不可欠な植物ホルモンである。CKは局所シグナルとして作用するだけでなく、維管束を介して長距離輸送され、器官間（長距離）シグナルとしても働く。本年度はCKの長距離輸送を介した個体統御メカニズムの解明を目指し、（1）CKの維管束への積み込みの分子メカニズムと（2）CK長距離輸送の生理的役割の理解を目指した研究を実施した。
    (1) CKの道管への積み込みを司る因子ABCG14について、生化学的特性と機能組織の詳細な解析を進めた。生化学的特性の解析ではin vitro輸送アッセイ系の構築を行った。試行錯誤の結果、無細胞タンパク質発現系で発現したABCG14を埋め込んだ人工膜を用いるアッセイ系の構築に成功した。また機能組織の解析については、細胞種特異的にABCG14を発現させた形質転換体シリーズの確立を完了し、道管液のCK量や分子種の変化、地上部の成長などの解析を進めている。ABCG14は維管束内の特定の細胞から前駆体を排出することにより、CKの道管への積み込みを司ることを強く示唆する結果を得た。
    (2) 地上部の炭素栄養条件に応じた個体成長統御に、CKの長距離輸送が関与することを明らかにして論文発表した（Sci. Rep. 2019）。また地上部から根へのCK情報の長距離伝搬のメカニズムとその生理的役割を明らかにするため、CK生合成関連変異体を用いて様々な組み合わせで接ぎ木を行った。接ぎ木植物のCK量や根の成長を評価した結果、地上部由来のCKには根の成長を抑制する働きがあることが示唆された。

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  • The molecular mechanism underlying nitrogen homeostasis in plants

    Grant number：17K07705  2017.04 - 2020.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    Kiba Takatoshi

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    Grant amount：\4680000 （ Direct expense: \3600000 、 Indirect expense：\1080000 ）

    Nitrogen homeostasis is crucial for plants to survive under fluctuating nutrient environments. It is maintained through the coordination of nitrogen-starvation and nitrogen-sufficiency responses. In this study, we identified a group of transcription factors, named NIGT1s, as a balancing factor between nitrogen-starvation and nitrogen-sufficiency responses. In addition, we revealed that NIGT1s act as a hub for integrating nitrogen and phosphorus signals to optimize nitrogen-phosphorus nutritional balance.

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  • Molecular mechanisms and physiological roles of long-distance cytokinin transport

    Grant number：16H01477  2016.04 - 2018.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    木羽 隆敏

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    Grant amount：\10530000 （ Direct expense: \8100000 、 Indirect expense：\2430000 ）

    サイトカイニン（CK）は植物の成長・発達の様々な過程の制御に不可欠な植物ホルモンであり、局所シグナルとして作用するだけでなく、維管束を介して長距離輸送され、器官間（長距離）シグナルとしても重要な役割をもつ。本研究では、CKの長距離輸送を介した個体統御メカニズムの解明を目指し、（1）CKの維管束への積み込みの分子メカニズムと（2）CK長距離輸送の生理的役割の観点からアプローチした。特に根から地上部への道管を介した長距離輸送に注目した。
    (1)ABCG14はCKの道管への積み込みに必須の因子であるが、基質特異性や輸送方向などの生化学的特性や機能組織など、詳しい働き方は分かっていない。我々はT87シロイヌナズナ培養細胞に誘導発現コンストラクトを導入した実験系を構築し、ABCG14がCK前駆体の排出に関わることを強く示唆する結果を得た。またabcg14背景でABCG14を組織特異的に発現する形質転換体を用いて表現型の回復を評価した結果、ABCG14が維管束で働くことが道管へのCK積み込みに重要であることが示唆された。ABCG14は維管束でCK前駆体を細胞外に排出することにより、道管へのCK積み込みを司ると考えられる。
    (2)根から地上部への道管を介したtZ型CKの輸送は、地上部の正常な成長・発達に欠かせない。道管液中の主要なCK分子種は前駆体tZRと活性型tZであるが、それらの生理的役割については不明であった。我々は、CK輸送・代謝系の変異体と接ぎ木を組み合わせることにより、tZRとtZの輸送の影響を区別して評価できる実験系を構築した。それにより、根由来のtZRとtZは異なった生理作用を持つことを明らかにし、論文化した。このようなCK分子種の使い分けは、環境変化に応じた個体統御システムを構成する重要なメカニズムの1つであると考えられる。

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  • Elucidation of the gene network underlying plant adaptation to chlonic nitrogen limitation

    Grant number：26712009  2014.04 - 2016.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (A)  Grant-in-Aid for Young Scientists (A)

    Kiba Takatoshi

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    Grant amount：\11180000 （ Direct expense: \8600000 、 Indirect expense：\2580000 ）

    This project aims at characterizing a family of GARP-type transcription factors (NBG), which were identified as regulators of a high-affinity nitrate transporter gene AtNRT2.4 playing a important role for efficient uptake of nitrogen under chlonic nitrogen limitation. Overexpression of NBG (NBGox) repressed AtNRT2.4 expression, while that of engineered NBG (NBG-VPox), which acts as an activator of transcription, induced it. NBGs are expressed throughout plant body being high under ample nitrogen and low under nitrogen limitation, suggesting that NBGs act as transcriptional repressors of not only AtNRT2.4 but also other genes involved in nitrogen-limitation responses. Comparative microarray analyses using NBGox and NBG-VPox suggest that NBGs function as one of master regulators of chronic nitrogen-limitation responses.

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  • Characterization of a set of novel bZIP transcription factors involved in nitrogen-starvation responses in plants

    Grant number：24780065  2012.04 - 2014.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    KIBA TAKATOSHI

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    Grant amount：\4420000 （ Direct expense: \3400000 、 Indirect expense：\1020000 ）

    I identified a set of GARP transcription factors as regulators of a high-affinity nitrate transporter gene AtNRT2.4, which is crucial for optimal adaptation to nitrogen starvation. I demonstrated that the GARP transcription factors act as transcriptional repressors to suppress AtNRT2.4 expression in nitrogen-replete conditions.

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  • Elucidation of carbon/nitrogen balance signal transduction system via plant hormones

    Grant number：21114005  2009.07 - 2014.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    SAKAKIBARA HITOSHI, KIBA Takatoshi, KAMADA-NOBUSADA Tomoe, KOJIMA Mikiko

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    Grant amount：\111930000 （ Direct expense: \86100000 、 Indirect expense：\25830000 ）

    We have demonstrated that enhanced production of trans-zeatin is a key factor to facilitate the shoot growth in response to high CO2 condition. IPT3 and CYP735A2 are regulated by CO2 concentration mediated by sugars produced from photosynthesis. We have demonstrated that the physiological function of tZ is distinct from that of iP, and the trans-hydroxylation of cytokinin controls shoot growth in Arabidopsis.
    We have shown that plants have multiple modes of regulation of de novo cytokinin synthesis in response to nitrogen status: Arabidopsis mainly uses nitrate-specific response system that regulates IPT3, and rice employs multiple IPT genes for nitrogen-dependent cytokinin synthesis, and glutamine metabolism is the signal for the regulation. In both cases, these regulations would be important for root-to-shoot supply of tZ via xylem for regulation of shoot growth.

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  • 高等植物におけるサイトカイニン応答分子機構の解明

    Grant number：05J04206  2005 - 2006

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    木羽 隆敏

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    Grant amount：\2300000 （ Direct expense: \2300000 ）

    サイトカイニン(CK)は形態形成、ストレス応答や栄養源の分配など植物の生育の様々な段階で重要な役割を果たす植物ホルモンである。しかしその受容・情報伝達機構については、数ある植物ホルモンの中でも最も研究が遅れていた。それがここ数年の間に急速に花開き、情報伝達モデルが提唱されるまでに進展した。しかしCK情報伝達については、その初期段階のフレームワークが明らかになったにすぎない。高等植物のCK応答分子機構の全容を解明するには、His-Asp系により制御される標的遺伝子の同定が鍵となると考えている。本研究はこの標的遺伝子の同定・機能解析から、高等植物におけるCK応答分子メカニズムの解明を目指すものである。そのために、以下に示す3段階の研究を計画した。マイクロアレイを用いた標的遺伝子の探索(研究1)、Chromatin immunoprecipitation (ChIP) assayを用いたType-B ARR標的遺伝子の探索(研究2)、研究1、2で同定した標的遺伝子の機能解析(研究3)である。
    平成17年度は主に研究1を遂行し、標的候補遺伝子の絞り込みを行った。その結果bHLH, Myb, AP2,GATAファミリーに属する転写因子、lateral organ boundary (LOB) domain containing proteinやhistone deacetylaseなど興味深い遺伝子を見いだすことに成功した。平成18年度は、これらの標的候補遺伝子の機能解析を行ってきた(研究3)。変異体や過剰発現体の表現形の解析から、いくつかの遺伝子について、His-Aspリン酸リレー情報伝達系の下流でサイトカイニン応答に関与することを示唆する結果が得られつつある。

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  • アラビドプシスにおけるサイトカイニンを介した窒素源情報伝達機構の解明

    Grant number：00J04303  2000 - 2002

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    木羽 隆敏

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    Grant amount：\3000000 （ Direct expense: \3000000 ）

    高等植物において、窒素栄養は最も欠乏しやすい栄養素の一つである。そのため、植物はその栄養状態を感知し、応答する機構を高度に発達させていると考えられる。近年、根圏における窒素栄養情報がサイトカイニンを介して地上部へと伝達される系が存在することが強く示唆された。また、そのサイトカイニンの情報伝達にはHis-Aspリン酸リレー系の関与が示唆されている。本研究ではHis-Aspリン酸リレー情報伝達系のレスポンスレギュレーターARRの機能解析から、高等植物の窒素情報伝達系の分子機構解明を目指した。特にその発現がサイトカイニンによって誘導されることが特徴であるタイプA ARRについて研究を進めた。
    サイトカイニンセンサーAHK4の下流でその発現が制御されるタイプA ARRの探索を行った。そしてAHK4機能欠損変異体cre1-1において、ARR15とARR16のサイトカイニン誘導性が顕著に低下することを明らかにした。AHK4とARR15、ARR16が共にサイトカイニン情報伝達系を構成することが示唆された。
    タイプA ARRであるARR15についてT-DNA挿入変異体を1ライン取得した。この変異体では、ARR15転写産物レベルが顕著に低下していた。その表現型をサイトカイニンに対する応答に焦点を当てて詳しく調べたが、特に変化は見られなかった。ARR15と重複した機能を果たす他のタイプA ARRが存在することが示唆された。
    ARR15についてその過剰発現体を作製して、その表現型の解析を行った。通常の栽培条件では、特にその形態に異常は見られなかった。サイトカイニンに対する応答に焦点を絞ってその表現型の解析を行ったところ、カルスの緑化及び根の伸長阻害実験から、サイトカイニンに対する感受性が特異的に低下していることを明らかにした。ARR15はサイトカイニンの情報伝達の負の出力調節機能をもつことが強く示唆された。
    ARR15はサイトカイニンセンサーAHK4によって発現誘導され、AHK4の下流のサイトカイニン情報伝達系のネガティブフィードバックループを形成すると考えられる。

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