2021/04/08 更新

写真a

カンザキ ヒロシ
神崎 浩
KANZAKI Hiroshi
所属
環境生命科学学域 教授
職名
教授
外部リンク

学位

  • 農学博士 ( 京都大学 )

研究キーワード

  • Enzyme Chemistry

  • 応用微生物学

  • Applied Microbiology

  • Natural Products Chemistry

  • 天然物化学

  • 酵素化学

研究分野

  • ナノテク・材料 / 生物分子化学

  • ライフサイエンス / 生物有機化学

  • ライフサイエンス / 応用生物化学

学歴

  • 京都大学    

    - 1986年

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    国名: 日本国

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  • 京都大学   Graduate School, Division of Agriculture  

    - 1986年

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  • 京都大学   農学部   農芸化学科

    - 1981年

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    国名: 日本国

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  • 京都大学   Faculty of Agriculture  

    - 1981年

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経歴

  • - Professor,Graduate School of Environmental and life Science,Okayama University

    2018年 - 現在

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  • - 岡山大学環境生命科学研究科 教授

    2018年 - 現在

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  • Executive Director and Vice President,Okayama University,Okayama University

    2017年 - 2018年

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  • 岡山大学

    2017年 - 2018年

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  • 岡山大学環境生命科学研究科 教授

    2012年 - 2017年

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  • Professor,Graduate School of Environmental and life Science,Okayama University

    2012年 - 2017年

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  • アメリカ公衆衛生研究所 博士研究員

    1988年 - 1989年

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  • 京都大学農学部 日本学術振興会特別研究員   Faculty of Agriculture

    1986年 - 1987年

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  • Postdoctoral Fellowships of Japan Society for the Promotion of Science

    1986年 - 1987年

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所属学協会

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委員歴

  • 日本農芸化学会   監事  

    2019年 - 現在   

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    団体区分:学協会

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  • 日本農芸化学会   広報委員会委員  

    2013年   

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    団体区分:学協会

    日本農芸化学会

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  • おかやまバイオアクティブ研究会   会長  

    2012年 - 現在   

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    団体区分:学協会

    おかやまバイオアクティブ研究会

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論文

  • Use of wood vinegar to enhance 5-aminolevulinic acid production by selected Rhodopseudomonas palustris in rubber sheet wastewater for agricultural use 査読

    Tomorn Nunkaew, Duangporn Kantachote, Sumate Chaiprapat, Teruhiko Nitoda, Hiroshi Kanzaki

    Saudi Journal of Biological Sciences25 ( 4 ) 642 - 650   2018年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier B.V.  

    This study aimed to produce inexpensive 5-aminolevulinic acid (ALA) in a non-sterile latex rubber sheet wastewater (RSW) by Rhodopseudomonas palustris TN114 and PP803 for the possibility to use in agricultural purposes by investigating the optimum conditions, and applying of wood vinegar (WV) as an economical source of levulinic acid to enhance ALA content. The Box–Behnken Design experiment was conducted under microaerobic-light conditions for 96 h with TN114, PP803 and their mixed culture (1:1) by varying initial pH, inoculum size (% v/v) and initial chemical oxygen demand (COD, mg/L). Results showed that the optimal condition (pH, % inoculum size, COD) of each set to produce extracellular ALA was found at 7.50, 6.00, 2000 for TN114
    7.50, 7.00, 3000 for PP803
    and 7.50, 6.00, 4000 for a mixed culture
    and each set achieved COD reduction as high as 63%, 71% and 75%, respectively. Addition of the optimal concentration of WV at mid log phase at 0.63% for TN114, and 1.25% for PP803 and the mixed culture significantly increased the ALA content by 3.7–4.2 times (128, 90 and 131 μM, respectively) compared to their controls. ALA production cost could be reduced approximately 31 times with WV on the basis of the amount of levulinic acid used. Effluent containing ALA for using in agriculture could be achieved by treating the RSW with the selected ALA producer R. palustris strains under the optimized condition with a little WV additive.

    DOI: 10.1016/j.sjbs.2016.01.028

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  • Administration of purple nonsulfur bacteria as single cell protein by mixing with shrimp feed to enhance growth, immune response and survival in white shrimp (Litopenaeus vannamei) cultivation 査読

    Supaporn Chumpol, Duangporn Kantachote, Teruhiko Nitoda, Hiroshi Kanzaki

    Aquaculture489   85 - 95   2018年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier B.V.  

    Single cell protein (SCP) is an alternative way to increase nutrients for animal consumption
    and purple nonsulfur bacteria (PNSB) should be considered as SCP due to their rich sources of protein, vitamins and photopigments. Hence, the aim of this study was to investigate the potential of promising PNSB to be used as SCP by mixing with commercial shrimp feed for white shrimp cultivation starting from postlarval until early juvenile stages for 60 days. PNSB strains, Rhodobacter sphaeroides SS15 and Afifella marina STW181 were selected, based on high amounts of biologically complete protein and photopigments, to use as SCP at a ratio of 1:1
    and their lyophilized cells at 1, 3 and 5% (w/w) were mixed well with commercial shrimp feed to obtain modified shrimp feed recipes
    Diet 1, Diet 2 and Diet 3, respectively. Levels of NH4 +, NO2 −, NO3 − and COD in rearing water from Diet 2 and 3 sets were significantly higher than control set. However, Diet 1 set showed the lowest levels of these water parameters among modified diet sets as no significant difference levels of NO3 − and COD between Diet 1 and control sets. Shrimp growth performance on the basis of relative gain rate and other growth parameters found that Diet 1 set was much better than sets of Diet 2, Diet 3 and control with the lowest found in the control set. In addition, the maximum shrimp survival was observed in Diet 1 set (85%) although no significant difference among them as 80% in control set. No significant difference was found among control and all modified shrimp diets for total hemocyte count in shrimp
    however, significant increases of superoxide dismutase activity found in sets of Diet 1, while phenoloxidase activity found in Diet 2 and Diet 3. The results of hepatopancreas (HP) histopathology analysis also showed a good condition of HP as healthy shrimp. PNSB biomass as SCP at optimal level has the potential to be an effective source of a novel protein in shrimp feed to enhance shrimp growth and also to increase shrimp survival as more nutritious with no effect on water quality.

    DOI: 10.1016/j.aquaculture.2018.02.009

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  • Unique antimicrobial spectrum of ophiobolin K produced by Aspergillus ustus 査読

    Natthapat Sohsomboon, Hiroshi Kanzaki, Teruhiko Nitoda

    Bioscience, Biotechnology and Biochemistry82 ( 3 ) 422 - 424   2018年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Japan Society for Bioscience Biotechnology and Agrochemistry  

    A co-cultivation study of two fungal strains showed that Aspergillus ustus could inhibit Aspergillus repens growth. The bioactive compound responsible for the observed activity was purified and identified as a sesterterpene, ophiobolin K. Ophiobolin K exhibited marked inhibition against both fungi and bacteria, especially A. repens, A. glaucus and gram-positive bacteria including Bacillus subtilis, Staphylococcus aureus, and Micrococcus luteus.

    DOI: 10.1080/09168451.2018.1429890

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  • In vitro and in vivo selection of probiotic purple nonsulphur bacteria with an ability to inhibit shrimp pathogens: acute hepatopancreatic necrosis disease-causing Vibrio parahaemolyticus and other vibrios 査読

    Supaporn Chumpol, Duangporn Kantachote, Pattamarat Rattanachuay, Varaporn Vuddhakul, Teruhiko Nitoda, Hiroshi Kanzaki

    AQUACULTURE RESEARCH48 ( 6 ) 3182 - 3197   2017年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Shrimp cultivation has been faced with huge losses in productivity caused by infectious shrimp pathogenic vibrios, especially Vibrio parahaemolyticus that causes acute hepatopancreatic necrosis disease (AHPND). Hence, purple nonsulphur bacteria (PNSB) were isolated from shrimp ponds for investigating their abilities to control shrimp pathogenic Vibrio spp. and their use as probiotics for sustainable shrimp cultivation. Based on their probiotic properties, strains S3W10 and SS15 were selected because of their strong abilities to produce amylase, gelatinase and vitamin B12. However, only three PNSB strains (SS15, TKW17 and STW181) strongly inhibited V. harveyi_KSAAHRC and V. vulnificus_KSAAHRC including V. parahaemolyticus AHPND strains by secreting antivibrio compounds. Four selected PNSB also grew in the presence of pancreatic enzymes, and they were identified as Rhodobacter sphaeroides for strains S3W10, SS15 and TKW17 and Afifella marina for strain STW181. The effects of a mixed culture were also investigated as follows: T1 (S3W10 + SS15), T2 (S3W10 + TKW17) and T3 (S3W10 + STW181) on postlarval white shrimp (Litopenaeus vannamei) for 60 days by comparison with a control. All three probiotic PNSB sets significantly improved the digestive enzyme activities and shrimp growth with their proliferation in shrimp gastrointestinal tract although the shrimp survival was not significantly different. They also significantly reduced the cumulative mortality of shrimp exposed to a virulent AHPND strain (V. parahaemolyticus SR2). This is the first to conclude that selected probiotic PNSB strains have great potential to be used for shrimp cultivation to control vibrios including AHPND strains.

    DOI: 10.1111/are.13149

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  • The roles of probiotic purple nonsulfur bacteria to control water quality and prevent acute hepatopancreatic necrosis disease (AHPND) for enhancement growth with higher survival in white shrimp (Litopenaeus vannamei) during cultivation 査読

    Supaporn Chumpol, Duangporn Kantachote, Teruhiko Nitoda, Hiroshi Kanzaki

    AQUACULTURE473   327 - 336   2017年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    This study aimed to investigate the potential of a mixed probiotic purple nonsulfur bacteria (PNSB) for controlling water quality and preventing acute hepatopancreatic necrosis disease (AHPND) to promote growth performance and increase survival of white shrimp (Litopenaeus vannamei) in postlarval stage during cultivation. Four probiotic PNSB (Rhodobacter sphaeroides strains SS15, S3W10, TKW17 and Afifella marina STW181) were investigated for controlling water quality using a mixed culture at a ratio of 1: 1 (roughly 1 x 10(8) cells mL(-1) for each, every week 1-7) as follows; T1 (S3W10 + SS15), T2 (S3W10 + TKW17) and T3 (S3W10 + STW181) and found that throughout 8 weeks shrimp cultivation the most effective sets to reduce NH4(+) and promote shrimp growth (based on weight and total length) were T1 and T3, respectively. Hence, PNSB strains (SS15, S3W10 and STW181) were used as a mixed culture (1: 1: 1, roughly 1 x 10(8) cells mL(-1) for each at weeks 1, 2 and 3) assessing their potential to prevent AHPND by challenge test on shrimp with a virulent strain AHPND-causing Vibrio parahaemolyticus SR2 (roughly 1 x 10(5) cells mL(-1) at day 15) during shrimp cultivation for 30 days. Inoculated PNSB sets (positive control: only PNSB inoculation, and treatment: both PNSB and SR2 inoculations) significantly decreased (P < 0.05) the levels of NH4-, NO2-, NO3- and chemical oxygen demand (COD) with significantly higher (P < 0.05) of dissolved oxygen (DO) compared to native control (no inoculation) and challenge test (only SR2 inoculation). The maximal growth performance of shrimp was observed in the positive control; while no significant difference (P > 0.05) was observed for other sets. PNSB survived and colonized in intestinal shrimp tract to prevent AHPND by increasing 11% survival rate of infected shrimp by strain SR2. The correlation coefficient between PNSB population and shrimp survival showed a positive strong correlation, but a negative strong correlation between vibrios population and shrimp survival. Overall results proved that a mixed three probiotic PNSB are a good candidate for applying in white shrimp cultivation to maintain water quality and to protect shrimp diseases for promoting shrimp growth with higher survival rate. (C) 2017 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.aquaculture.2017.02.033

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  • Selection of salt tolerant purple nonsulfur bacteria producing 5-aminolevulinic acid (ALA) and reducing methane emissions from microbial rice straw degradation 査読

    Tomorn Nunkaew, Duangporn Kantachote, Teruhiko Nitoda, Hiroshi Kanzaki

    APPLIED SOIL ECOLOGY86   113 - 120   2015年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Saline soil is one major problem that causes low productivity on rice, while paddy fields are also one source of methane (CH4) emissions that can have a considerable impact on global warming. By using purple nonsulfur bacteria (PNSB) it is possible to overcome both these serious problems because PNSB produce 5-aminolevulinic acid (ALA), a compound that reduces salt stress for plants, and they are also good competitors for methanogens in the paddy fields. Therefore, the objective of this study was to select salt resistant PNSB with an ability to release ALA and reduce CH4 emissions. The PNSB isolates used were obtained from saline paddy fields in southern Thailand and the best were selected on the basis of their good growth under conditions of aerobic dark and anaerobic light while being salt tolerant, released ALA and reduced CH4 emissions under microaerobic light conditions. Among the 272 PNSB isolates, TN114 was the best ALA producer while PP803 was the best to reduce CH4 emissions. Both selected PNSB strains grew well in glutamate acetate (GA) broth containing NaCl up to 6.00% but 0.25% NaCl was their optimal concentration for growth. Under salt stress with 0.25% NaCl, the strain TN114 released 25.63 mu M ALA in GA broth. In scaled-up experiments, when it was mixed with native soil flora in rice straw broth the strain TN114 released 13.44 mu M ALA and the strain PP803 reduced CH4 emissions by 88.41%. These two strains were identified as Rhodopseudomonas palustris according to both traditional and 16 S rRNA identifications. Overall the results demonstrated that they had a good potential as plant growth stimulating bacteria under salt stress, due to the release of ALA and also reduce CH4 emissions. (C) 2014 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.apsoil.2014.10.005

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  • The use of rice straw broth as an appropriate medium to isolate purple nonsulfur bacteria from paddy fields 査読

    Tomorn Nunkaew, Duangporn Kantachote, Teruhiko Nitoda, Hiroshi Kanzaki

    ELECTRONIC JOURNAL OF BIOTECHNOLOGY15 ( 6 )   2012年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:UNIV CATOLICA DE VALPARAISO  

    The aims were to explore an appropriate isolating medium for obtaining purple nonsulfur bacteria (PNSB) for use as biofertilizers in saline paddy fields and to obtain pure cultures. We therefore chose a defined isolating medium containing 0.25% NaCl, (Glutamate-Acetate broth, GA) and a rice straw broth to compare them for numbers of PNSB obtained, time to obtain pure cultures, diversity and costs. A total of 30 water and 30 sediment samples were collected from saline paddy fields in southern Thailand and used to isolate PNSB in both the isolating media. Based on 60 samples and a period of 13 days incubation under anaerobic light conditions, a greater number of samples produced PNSB growth in GA broth after only day 3; however, after that the rice straw broth provided about a 2 fold increase in the number of samples that produced PNSB growth. Colonies isolated from GA broth required a significantly higher number of repeated streaking to obtain a pure culture (average 3.5) than those from rice straw broth (average 2.7) and the latter medium also produced significantly (P < 0.05) more isolates per sample. Sixty samples of water and sediment, from rice paddies with salinity (average, 3.43 +/- 0.67 mS/cm) and slight acidity (average, pH 5.84 +/- 0.42) provided 62 PNSB isolates by GA broth and 210 isolates by rice straw broth, and rice straw broth also produced a greater prevalence of PNSB. Estimates of the costs based on current prices of media, Gas Pak and electricity to obtain PNSB with the use of GA broth was roughly 6 times higher than for the rice straw broth.

    DOI: 10.2225/vol15-issue6-fulltext-8

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  • Erratum to "Chemical structures and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste" [Bioresour. Technol. 98(5) (2007) 1149-1153] (DOI:10.1016/j.biortech.2005.10.045) 査読

    Benjamas Thanomsub, Wanna Pumeechockchai, Anirut Limtrakul, Panarat Arunrattiyakorn, Wipawan Petchleelaha, Teruhiko Nitoda, Hiroshi Kanzaki

    Bioresource Technology100 ( 23 ) 6141   2009年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.biortech.2009.04.058

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  • Chemical structures and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste (Retracted Article. See vol 100, pg 6141, 2009) 査読

    Benjamas Thanomsub, Wanna Pumeechockchai, Anirut Limtrakul, Panarat Arunrattiyakorn, Wipawan Petchleelaha, Teruhiko Nitoda, Hiroshi Kanzaki

    BIORESOURCE TECHNOLOGY98 ( 5 ) 1149 - 1153   2007年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI LTD  

    The aim of this work was to study chemical structures and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste. The culture produced two biosurfactants, a and b, which showed strong activity and were identified as L-rhamnopyranosyl-(L)-rhamnopyranosyl-beta-hydroxydecanoyl-beta-hydroxydecanoate or Rha-Rha-C-10-C-10 and (L)-rhamnopyranosyl-(L)-rhamnopyranosyl-beta-hydroxydecanoyl-beta-hydroxydodecanoate or Rha-Rha-C-10-C-12, respectively. Both compounds exhibited higher surfactant activities tested by the drop collapse test than several artificial surfactants such as SDS and Tween 80. Rhamnolipid a showed significant antiproliferative activity against human breast cancer cell line (MCF-7) at minimum inhibitory concentration (MIC) at 6.25 mu g/mL while rhamnolipid b showed MIC against insect cell line C6/36 at 50 mu g/mL. (c) 2006 Published by Elsevier Ltd.

    DOI: 10.1016/j.biortech.2005.10.045

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  • Chemical structures and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste 査読

    Benjamas Thanomsub, Wanna Pumeechockchai, Anirut Limtrakul, Panarat Arunrattiyakorn, Wipawan Petchleelaha, Teruhiko Nitoda, Hiroshi Kanzaki

    BIORESOURCE TECHNOLOGY97 ( 18 ) 2457 - 2461   2006年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI LTD  

    The aim of this work was to study chemical structures and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste. The culture produced two biosurfactants, a and b, which showed strong activity and were identified as L-rhamnopyranosyl-L-rhamnopyranosyl-beta-hydroxydecanoyl-beta-hydroxydecanoate or Rha-Rha C-10-C-10 and L-rhamnopyranosyl-L-rhamnopyranosyl-beta-hydroxydecanoyl-beta-hydroxydodecanoate or Rha-Rha C-10-C-12, respectively. Both compounds exhibited higher surfactant activities tested by the drop collapse test than several artificial surfactants such as SDS and Tween 80. Rhamnolipid a showed significant antiproliferative activity against human breast cancer cell line (MCF-7) at minimum inhibitory concentration (MIC) at 6.25 mu g/mL while rhamnolipid b showed MIC against insect cell line C6/36 at 50 mu g/mL. (c) 2005 Published by Elsevier Ltd.

    DOI: 10.1016/j.biortech.2005.10.029

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  • Novel bioactive oxazolomycin isomers produced by Streptomyces albus JA3453 査読

    H Kanzaki, K Wada, T Nitoda, K Kawazu

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY62 ( 3 ) 438 - 442   1998年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Two novel oxazolomycin isomers, oxazolomycins B (2) and C (3), were isolated from the fermentation broth of an oxazolomycin-producing strain, Streptomyces albus JA3453. Both compounds are geometrical isomers of oxazolomycin (1), the configurations of their triene moieties being (4'E, 6'E, 8'E) (2) and (4'Z, 6'E, 8'E) (3) while that of oxazolomycin fl) is (4'Z, 6'Z, 8'E), Compounds 2 and 3 exhibited potent inhibitory activity against crown gall formation with the same MIC (0.8 mu g/disk) as oxazolomycin. Compounds 2 and 3 showed no antibacterial activity against Agrobacterium tumefaciens, in contrast to oxazolomycin which has specific anti-A. tumefaciens activity.

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  • ELICITOR-ACTIVE OLIGOSACCHARIDES FROM ALGAL LAMINARAN STIMULATE THE PRODUCTION OF ANTIFUNGAL COMPOUNDS IN ALFALFA 査読

    A KOBAYASHI, A TAI, H KANZAKI, K KAWAZU

    ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES48 ( 7-8 ) 575 - 579   1993年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:VERLAG Z NATURFORSCH  

    Natural polysaccharides were examined for their activity as elicitors of phytoalexins and flavonoids in alfalfa cotyledons. Only two polysaccharides, laminaran and pectic acid, had elicitor activity. Laminaran, which was more active than pectic acid, was hydrolyzed by tunicase and the hydrolysate was subjected to charcoal and gel filtration columns. Introduction of the pyridylamino group into the elicitor-active oligosaccharides was attempted in order to facilitate isolation. The pyridylamino derivatives were found to exhibit higher activity than the original oligosaccharides. Their liquid chromatography mass spectrometry (LC-MS) analysis revealed that the elicitor-active principles form two ion clusters with the same molecular weights, m/z 1070 and 1232. Their high performance liquid chromatography (HPLC) analysis showed three main peaks. The individual peaks (LN-1, 2,3) were collected and subjected to the alfalfa cotyledon assay. LN-3 showed the highest activity (minimum effective concentration, 0.8 mug/ml).

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MISC

  • Characterization of exopolymeric substances from selected Rhodopseudomonas palustris strains and their ability to adsorb sodium ions

    Tomorn Nunkaew, Duangporn Kantachote, Teruhiko Nitoda, Hiroshi Kanzaki, Raymond J. Ritchie

    CARBOHYDRATE POLYMERS115 ( 22 ) 334 - 341   2015年1月

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    記述言語:英語   出版者・発行元:ELSEVIER SCI LTD  

    Removal of Na+ by binding with exopolymeric substances (EPS) from Rhodopseudomonas palustris TN114 and PP803 was investigated. The moderate negative correlation pairs (r(p)) between remaining Alcian blue and amount of Na+ adsorbed on EPS from strains TN114 and PP803 were -0.652 and -0.609. Both strains showed positive relationships between the amounts of EPS produced and bacterial growth. EPS from strain PP803 had a higher efficiency in removing Na+ than the EPS from strain TN114 based on their EC50 values (1.79 and 1.49 mg/mL for TN114 and PP803, respectively). The principal component from EPS of strain PP803 which was responsible for salt removal was purified and it was identified as a polysaccharide kDa) mainly composed of galacturonic acid. Overall results suggested that EPS is a key factor that our strains used to bind Na* allowing their survival in high NaC1 concentrations. (C) 2014 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.carbpol.2014.08.099

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  • Biotransformation of α-mangostin by Colletotrichum sp. MT02 and Phomopsis euphorbiae K12

    Panarat Arunrattiyakorn, Nuttika Suwannasai, Thammarat Aree, Somdej Kanokmedhakul, Hideyuki Ito, Hiroshi Kanzaki

    Journal of Molecular Catalysis B: Enzymatic102   174 - 179   2014年4月

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  • Effects of 5-aminolevulinic acid (ALA)-containing supernatants from selected Rhodopseudomonas palustris strains on rice growth under NaCl stress, with mediating effects on chlorophyll, photosynthetic electron transport and antioxidative enzymes

    Tomorn Nunkaew, Duangporn Kantachote, Hiroshi Kanzaki, Teruhiko Nitoda, Raymond J. Ritchie

    ELECTRONIC JOURNAL OF BIOTECHNOLOGY17 ( 1 ) 19 - 26   2014年1月

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    記述言語:英語   出版者・発行元:UNIV CATOLICA DE VALPARAISO  

    Background: Rice is globally one of the most important food crops, and NaCl stress is a key factor reducing rice yield. Amelioration of NaCl stress was assessed by determining the growth of rice seedlings treated with culture supernatants containing 5-aminolevulinic acid (ALA) secreted by strains of Rhodopseudomonas palustris (TN114 and PP803) and compared to the effects of synthetic ALA (positive control) and no ALA content (negative control).
    Results: The relative root growth of rice seedlings was determined under NaCl stress (50 mM NaCl), after 21 d of pretreatment. Pretreatments with 1 mu M commercial ALA and 10X diluted culture supernatant of strain TN114 (2.57 mu M ALA) gave significantly better growth than 10X diluted PP803 supernatant (2.11 mu M ALA). Rice growth measured by dry weight under NaCl stress ordered the pretreatments as: commercial ALA N TN114 N PP803 N negative control. NaCl stress strongly decreased total chlorophyll of the plants that correlated with non-photochemical quenching of fluorescence (NPQ). The salt stress also strongly increased hydrogen peroxide (H2O2) concentration in NaCl-stressed plants. The pretreatments were ordered by reduction in H2O2 content underNaCl stress as: commercial ALA N TN114 N PP803 N negative control. The ALA pretreatments incurred remarkable increases of total chlorophyll and antioxidative activities of catalase (CAT), ascorbate peroxide (APx), glutathione reductase (GR) and superoxide dismutase (SOD); under NaCl stress commercial ALA and TN114 had generally stronger effects than PP803.
    Conclusions: The strain TN114 has potential as a plant growth stimulating bacterium that might enhance rice growth in saline paddy fields at a lower cost than commercial ALA. (C) 2014 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.ejbt.2013.12.004

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  • Total synthesis of the proposed structure for pochonicine and determination of its absolute configuration

    Yujiro Kitamura, Hiroyuki Koshino, Takemichi Nakamura, Aya Tsuchida, Teruhiko Nitoda, Hiroshi Kanzaki, Koji Matsuoka, Shunya Takahashi

    Tetrahedron Letters54 ( 11 ) 1456 - 1459   2013年3月

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    記述言語:英語   出版者・発行元:Elsevier Ltd  

    Pochonicine is a polyhydroxylated pyrrolizidine alkaloid with a powerful inhibitory activity against β-Nacetylglucosaminidases. The proposed structure for pochonicine and the three diastereomers concerning its C-1 and/or C-3 positions were synthesized from N-acetyl-D-glucosamine through construction of the pyrrolizidine skeleton by two intramolecular amino cyclizations as key steps. This synthetic study not only revised the structure of the natural product to the corresponding 1,3-di-epi-form but also determined the absolute configuration as 1R, 3S, 5R, 6R, 7S, 7aR. Copyright © 2012 Published by Elsevier Ltd.

    DOI: 10.1016/j.tetlet.2013.01.015

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  • TMG-chitotriomycin as a probe for the prediction of substrate specificity of β-N-acetylhexosaminidases

    Hiroto Shiota, Hiroshi Kanzaki, Tadashi Hatanaka, Teruhiko Nitoda

    Carbohydrate Research375   29 - 34   2013年

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  • Microbial metabolism of α-mangostin isolated from Garcinia mangostana L.

    Panarat Arunrattiyakorn, Sunit Suksamrarn, Nuttika Suwannasai, Hiroshi Kanzaki

    Phytochemistry72 ( 8 ) 730 - 734   2011年6月

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  • Antivibrio compounds produced by Pseudomonas sp W3: characterisation and assessment of their safety to shrimps

    Pattamarat Rattanachuay, Duangporn Kantachote, Manee Tantirungkij, Teruhiko Nitoda, Hiroshi Kanzaki

    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY27 ( 4 ) 869 - 880   2011年4月

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    記述言語:英語   出版者・発行元:SPRINGER  

    In order to explore compounds naturallly inhibitory to shrimp pathogenic vibrios, a culture filtrate of Pseudomonas sp. W3 at a pH of 2 was extracted with ethyl acetate (EtOAc) to produce 82.15 mg/l of a yellow-brown extract (EtOAc-W3) that had MIC values of 225-450 mu g/ml against the growth of 18 shrimp pathogenic Vibrio harveyi strains. The MIC of EtOAc-W3 against the most pathogenic strain PSU 2015 was 450 mu g/ml and this strain had the lowest LD(50) (50% lethal dose) to pacific white shrimp (Litopenaeus vannamei, PL 21). At this MIC value, EtOAc-W3 in artificial sea water (ASW) killed strain PSU 2015; however in natural sea water, only a partial growth inhibition was observed. The toxicity to pacific white shrimp and antivibrio activity of the EtOAc-W3 were investigated by conducting an experiment with 4 sets; native control (commercial ASW), EtOAc-W3 control (MIC/10, 45 mu g/ml), challenge (inoculation 6.0 x 10(6) c.f.u./ml PSU 2015) and treatment (6.0 x 10(6) c.f.u./ml PSU 2015 + 45 mu g/ml EtOAc-W3). The same experiment was repeated by increasing the dose of EtOAc-W3 to 90 mu g/ml (MIC/5). Both concentrations of EtOAc-W3 tested had no toxicity to postlarval shrimps. A significant decrease in shrimp mortality was observed over a 72 h period as approximately 80% of the shrimps died in each challenge set but only 63 and 23% died in the presence of 45 and 90 mu g/ml EtOAc-W3. The major component of EtOAc-W3 was supposed to be 2-heptyl-4-quinolone (HHQ) by FAB-MS and (1)H-NMR analyses of the purified fraction.

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  • MS/MS fragmentation-guided search of TMG-chitooligomycins and their structure-activity relationship in specific β-N-acetylglucosaminidase inhibition

    Hirokazu Usuki, Yukihiro Yamamoto, Yuya Kumagai, Teruhiko Nitoda, Hiroshi Kanzaki, Tadashi Hatanaka

    Organic & Biomolecular Chemistry9 ( 8 ) 2943 - 2951   2011年

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  • Characterization of the antiyeast compound and probiotic properties of a starter Lactobacillus plantarum DW3 for possible use in fermented plant beverages

    Duangporn Kantachote, Pakorn Prachyakij, Wilawan Charernjiratrakul, Metta Ongsakul, Yodsawee Duangjitcharoen, Chaiyavat Chaiyasut, Teruhiko Nitoda, Hiroshi Kanzaki

    ELECTRONIC JOURNAL OF BIOTECHNOLOGY13 ( 5 )   2010年9月

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    記述言語:英語   出版者・発行元:UNIV CATOLICA DE VALPARAISO  

    Lactobacillus plantarum DW3 produced antifungal compounds that inhibited the growth of Rhodotorula mucilaginosa DKA, contaminating yeast in fermented plant beverages (FPBs) and various potential human pathogens. Phenyllactic acid (PLA) identified by gas chromatography- mass spectrometry (GC-MS) was produced at 31 mg/L PLA in MRS medium and 5 mg/ml inhibited growth of the target yeast in vitro by 90%. Other inhibitors were also present but not specifically identified. Results of in vitro tests showed that DW3 also had probiotic properties as it survived various human biological barriers resistance to pH 3, bile salts, growth without vitamin B(12) and the presence and absence of oxygen. Its inhibitory effect against foodborne pathogenic bacteria and spoilage organisms was higher than that found for a commercial strain Lactobacillus casei R. An acute oral toxicity test on ICR mice at a high single dose of either 10(9) and 10(12) cells per mouse for 14 days showed that DW3 had no adverse effect on the general health status and there was no evidence of bacteremia. Mice fed DW3 had a reduced weight gain compared to the control. No significant difference (p > 0.05) was found for the spleen weight index (SWI) among the treatment and control groups whereas there was a significant difference (p < 0.05) for the liver weight ratio (LWR) in a group fed with 10(12) cells per mouse when compared with the control group.

    DOI: 10.2225/vol13-issue5-fulltext-1

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  • Conversion of secondary metabolites from olive leaves by Baker's yeasts and the antioxidative activity of the product

    Erika Akihisa, Yoshihiro Harada, Kumi Kobayashi, Akiko Hirano, Teruhiko Nitoda, Hiroshi Kanzaki

    JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC62 ( 1 ) 123 - 123   2010年1月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ELSEVIER SCIENCE BV  

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  • Inhibition of shrimp pathogenic vibrios by extracellular compounds from a proteolytic bacterium Pseudomonas sp W3

    Pattamarat Rattanachuay, Duangporn Kantachote, Manee Tantirungkij, Teruhiko Nitoda, Hiroshi Kanzaki

    ELECTRONIC JOURNAL OF BIOTECHNOLOGY13 ( 1 )   2010年1月

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    記述言語:英語   出版者・発行元:UNIV CATOLICA DE VALPARAISO  

    Pseudomonas sp. W3, a bacterium known to produce an extracellular alkaline protease, secreted secondary metabolites that inhibited pathogenic bacteria responsible for shrimp luminous vibriosis disease. Antivibrio compounds in the culture supernatant or culture filtrates (0.45 mu m and 0.22 mu m) of the isolate W3 were tested using an agar well diffusion method on a number of pathogenic vibrios. Vibrio harveyi PSU 2015 a pathogenic isolate was the most sensitive strain. The effectiveness of preparations from the isolate W3 against V. harveyi PSU 2015, and V. cholerae PSSCMI 0062 was in the order of culture supernatant > 0.45 mu m culture filtrate > 0.22 mu m culture filtrate. These extracellular antivibrio compounds also lysed both dead and living cells of V. harveyi PSU 2015. Results of the partial characterization tests indicated that there was some particulate antivibrio compound that was destroyed by treatment with enzymes particularly achymotrypsin, autoclaving at 121 degrees C for 15 min and was mostly removed by filtration through a 0.22 mu m filter. Most of the inhibitory compounds were of small molecular weight able to pass through a 0.22 mu m filter and were resistant to treatment with various enzymes, pH values between 4-8 and temperatures up to 121 degrees C for 30 min. The optimum pH for the antivibrio activity in the 0.45 mu m culture filtrate was between pH 6-7.

    DOI: 10.2225/vol13-issue1-fulltext-2

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  • Chemical structures and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste (Retraction of vol 98, pg 1149, 2007)

    Benjamas Thanomsub, Wanna Pumeechockchai, Anirut Limtrakul, Panarat Arunrattiyakorn, Wipawan Petchleelaha, Teruhiko Nitoda, Hiroshi Kanzaki

    BIORESOURCE TECHNOLOGY100 ( 23 ) 6141 - 6141   2009年12月

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    記述言語:英語   出版者・発行元:ELSEVIER SCI LTD  

    DOI: 10.1016/j.biortech.2009.04.058

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  • Pochonicine, a polyhydroxylated pyrrolizidine alkaloid from fungus Pochonia suchlasporia var. suchlasporia TAMA 87 as a potent β-N-acetylglucosaminidase inhibitor

    Hirokazu Usuki, Miho Toyo-oka, Hiroshi Kanzaki, Toru Okuda, Teruhiko Nitoda

    Bioorganic & Medicinal Chemistry17 ( 20 ) 7248 - 7253   2009年10月

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  • Bioassays: inhibitors of insect chitin-degrading enzymes

    Teruhiko Nitoda, Hiroshi Kanzaki

    Isolation, Identification and Characterization of Allelochemicals/Natural Products   499 - 412   2009年

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  • 生物種の由来が異なる酵素を見分ける阻害剤 新規な構造と活性をもつ放線菌由来β-N-アセチルグルコサミニダーゼ阻害剤

    仁戸田 照彦, 臼木 博一, 神崎 浩

    化学と生物47 ( 3 ) 158 - 160   2009年

  • TMG-chitotriomycin, an enzyme inhibitor specific for insect and fungal beta-N-acetylglucosaminidases, produced by actinomycete Streptomyces anulatus NBRC 13369

    Hirokazu Usuki, Teruhiko Nitoda, Misato Ichikawa, Nahoko Yamaji, Takashi Iwashita, Hajime Komura, Hiroshi Kanzaki

    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY130 ( 12 ) 4146 - 4152   2008年3月

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    記述言語:英語   出版者・発行元:AMER CHEMICAL SOC  

    A novel beta-N-acetylglucosaminidase (GIcNAcase) inhibitor named TMG-chitotriomycin (1) was isolated from the culture filtrate of Streptomyces anulatus NBRC13369. The strain produced 1 only when colloidal chitin was used as the sole carbon source in the production medium. The structure of 1 was determined by spectral and constitutive sugar analyses of the corresponding alditol derivatives to be an equilibrated mixture of alpha-D-N,N,N-triMeGIcNH(2)-(1,4)-beta-D-GIcNAc-(1,4)-beta-D-GIcNAc-(1,4)-D-GlcNAc and its C-2 epimer of the reducing end residue. TMG-chitotriomycin (1) showed potent and selective inhibition of insect and fungal GIcNAcases with no inhibition of mammalian and plant GIcNAcases. In contrast, the known GIcNAcase inhibitor nagstatin potently inhibited all GIcNAcases. It should be emphasized that synthesized D-N,N,N-triMeGIcNH(2), which is the component sugar of 1, showed no inhibition of the insect Spodoptera litura GIcNAcase. These results suggest that the (GIcNAc)(3) unit positioned at the reducing end of 1 is essential for its enzyme inhibitory activity. The unique inhibitory spectrum of 1 will be useful to study chitinolytic systems and to develop selective fungicides or pesticides.

    DOI: 10.1021/ja077641f

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  • Purification and characterization of a biosurfactant produced by Issatchenkia orientalis SR4

    Wichuda Katemai, Suppasil Maneerat, Fusako Kawai, Hiroshi Kanzaki, Teruhiko Nitoda, Aran H-Kittikun

    JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY54 ( 1 ) 79 - 82   2008年2月

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    記述言語:英語   出版者・発行元:MICROBIOL RES FOUNDATION  

    DOI: 10.2323/jgam.54.79

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  • Antinematodal activities of ingenane diterpenes from Euphorbia kansui and their derivatives against the pine wood nematode (Bursaphelenchus xylophilus)

    Jianxiao Shi, Zhixuan Li, Teruhiko Nitoda, Minoru Izumi, Hiroshi Kanzaki, Naomichi Baba, Kazuyoshi Kawazu, Shuhei Nakajima

    ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES63 ( 1-2 ) 59 - 65   2008年1月

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    記述言語:英語   出版者・発行元:VERLAG Z NATURFORSCH  

    Under the bioassay-guided method, two diterpenes, 3-O-(2",3"-dimethylbutanoyl)-13-O-dodecanoylingenol (1) and 3-O-(2",3"-dimethylbutanoyl)-13-O-decanoylingenol (2) isolated from Euphorbia kansui, showed a pronounced antinematodal activity against the nematode Bursaphelenchus xylophilus at the same minimum effective dose (MED) of 5 mu g per cotton ball and still displayed antinematodal activity at a dose of 2.5 mu g per cotton ball. Compounds 3-6 were obtained, and the structure of the new compound 6 was elucidated based on 1D- and 2D-NMR analyses and physicochemical data. Preliminary structure-biological activity relationships of ingenane-type compounds were deduced.

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  • Antinematodal activities of ingenane diterpenes from Euphorbia kansui and their derivatives against the pine wood nematode (Bursaphelenchus xylophilus)

    Jianxiao Shi, Zhixuan Li, Teruhiko Nitoda, Minoru Izumi, Hiroshi Kanzaki, Naomichi Baba, Kazuyoshi Kawazu, Shuhei Nakajima

    ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES63 ( 1-2 ) 59 - 65   2008年1月

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    記述言語:英語   出版者・発行元:VERLAG Z NATURFORSCH  

    Under the bioassay-guided method, two diterpenes, 3-O-(2",3"-dimethylbutanoyl)-13-O-dodecanoylingenol (1) and 3-O-(2",3"-dimethylbutanoyl)-13-O-decanoylingenol (2) isolated from Euphorbia kansui, showed a pronounced antinematodal activity against the nematode Bursaphelenchus xylophilus at the same minimum effective dose (MED) of 5 mu g per cotton ball and still displayed antinematodal activity at a dose of 2.5 mu g per cotton ball. Compounds 3-6 were obtained, and the structure of the new compound 6 was elucidated based on 1D- and 2D-NMR analyses and physicochemical data. Preliminary structure-biological activity relationships of ingenane-type compounds were deduced.

    DOI: 10.1515/znc-2008-1-211

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  • 岡山大産米100%使用,日本酒「おお岡大」― 学産学消から学産地消を目指して ―

    神崎浩

    日本生物工学会誌86 ( 5 ) 250 - 251   2008年

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  • 微生物変換による新しい天然由来の化粧品素材開発を目指して―オリーブ葉由来抗酸化成分の微生物による高機能化―

    神崎 浩, 仁戸田照彦

    FRAGRANCE JOURNAL36 ( 2 ) 63 - 66   2008年

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  • Purification and characterization of cyclo (Leu-Phe) oxidase of Streptomyces albulus expressed in Escherichia coli

    Machiko Nagao, Rie Hirata, Teruhiko Nitoda, Hiroshi Kanzaki

    JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC48 ( 3-4 ) 108 - 108   2007年9月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ELSEVIER SCIENCE BV  

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  • Three antinematodal diterpenes from Euphorbia kansui

    Jian-Xiao Shi, Zhi-Xuan Li, Teruhiko Nitoda, Minoru Izumi, Hiroshi Kanzaki, Naomichi Baba, Kazuyoshi Kawazu, Shuhei Nakajima

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY71 ( 4 ) 1086 - 1089   2007年4月

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    記述言語:英語   出版者・発行元:TAYLOR & FRANCIS LTD  

    Three compounds, 20-O-acetyl-[3-O-(2'E,4'Z)-deca-dienoyl]-ingenol (1), 20-O-acetyl-[5-O-(2'E,4'Z)-decadienoyl]-ingenol (2) and 3-O-(2'E,4'Z)-decadienoylingenol (3), were isolated from Euphorbia kansui under the bioassay-guided method. Each compound showed the same antinematodal activity against the nematode, Bursaphelenchus xylophilus, at a minimum effective dose (MED) of 5 mu g/cotton ball.

    DOI: 10.1271/bbb.60677

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  • Enzymatic synthesis of dehydroderivatives from proline-containing cyclic dipeptides and their effects toward cell division

    Panarat Arunrattiyakorn, Banri Ikeda, Teruhiko Nitoda, Hiroshi Kanzaki

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY71 ( 3 ) 830 - 833   2007年3月

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    記述言語:英語   出版者・発行元:TAYLOR & FRANCIS LTD  

    We have previously isolated cyclo(L-PrO-L-Tyr) and cyclo(L-Phe-L-Pro) from an actinomycete by a novel enzymatic conversion-guided method. Their tetradehydro derivatives, cyclo(Delta Pro-Delta Tyr) and cyclo(Delta Phe-Delta Pro), were enzymatically prepared. Neither of them inhibited cell division, in contrast to other tetradehydro cyclic dipeptides prepared previously. This result suggests that an NH proton in a diketopiperazine ring and/or conformation of the compound are important for the activity.

    DOI: 10.1271/bbb.60651

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  • Microorganisms with homocysteine thiolactone hydrolase: Screening and stereospecificity - Abstracts

    Shozo Honda, Teruhiko Nitoda, Hiroshi Kanzaki

    JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC42 ( 3-4 ) 126 - 127   2006年11月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ELSEVIER SCIENCE BV  

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  • Further proof for wide substrate specificity of cyclo(Leu-Phe) oxidase from an actinomycete: Enzymatic conversion of cyclic dipeptides containing non-aromatic amino acids - Abstracts

    Rie Hirata, Teruhiko Nitoda, Hiroshi Kanzaki

    JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC42 ( 3-4 ) 127 - 128   2006年11月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ELSEVIER SCIENCE BV  

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  • Enzymatic conversion-based method for screening cyclic dipeptide-producing microbes

    P Arunrattiyakorn, T Nitoda, H Kanzaki

    PEPTIDES27 ( 4 ) 633 - 639   2006年4月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE INC  

    We developed a method for screening cyclic dipeptide-producing microbes by enzymatic conversion. In this method, cyclic dipeptides are detected by the combination of: (i) conversion of cyclic dipeptides to dehydro cyclic dipeptides by cyclo(Leu-Phe) oxidase and (ii) detection of the dehydro derivative by UV spectrophotometry using TLC or HPLC analysis based on the absorbance change caused by the conversion. Using this method, the actinomycete strain A8 was isolated as a cyclic dipeptide-producing strain. The cyclic dipeptides were purified from the microbial extract by enzymatic detection-guided fractionation, and their structures were determined to be CYCIO(L-Phe-L-Pro) and cycIO(L-Pro-L-Tyr) by spectroscopic methods. (c) 2005 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.peptides.2005.08.017

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  • Activation of murine peritoneal macrophages by water-soluble extracts of Bursaphelenchus xylophilus, a pine wood nematode

    H Kaji, A Tai, K Matsushita, H Kanzaki, Yamamoto, I

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY70 ( 1 ) 203 - 210   2006年1月

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    記述言語:英語   出版者・発行元:TAYLOR & FRANCIS LTD  

    In our previous study, water-soluble extracts from Bursaphelenchus xylophilus (B. xylophilus), a pine wood nematode, were shown to enhance interleukin (IL)-4 plus lipopolysaccharide-induced polyclonal immunoglobulin (Ig) E production in vitro in mice and to increase serum levels of an antigen-nonspecific IgE in vivo. Here we examined whether the nematode extracts stimulate immunofunctions of murine peritoneal macrophages. In both resident and inflammatory macrophages, Fey receptor-mediated phagocytosis was markedly activated by B. xylophilus extracts, while non-specific phagocytosis was not. The enhancement of specific phagocytosis was accompanied by an increase in the formation of IgG-Fc gamma receptor rosettes. B. xylophilus extracts also stimulated IL-1 beta production in both types of macrophages, and enhanced NO production and mRNA expression of inflammatory cytokines in inflammatory macrophages. These results indicate that the extracts of B. xylophilus contain an activating substance(s) for immunofunctions in macrophages, besides an enhancing factor for polyclonal IgE production.

    DOI: 10.1271/bbb.70.203

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  • Screening and partial characterization of inhibitors of insect β-N-acetylglucosaminidase.

    Usuki, H, Nitoda, T, Okuda, T, Kanzaki, H

    Journal of Pesticide Science31 ( 1 ) 41 - 46   2006年

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  • Bile acids are new products of a marine bacterium, Myroides sp strain SM1

    S Maneerat, T Nitoda, H Kanzaki, F Kawai

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY67 ( 5 ) 679 - 683   2005年6月

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    記述言語:英語   出版者・発行元:SPRINGER  

    Strain SM1 was isolated as a biosurfactant-producing microorganism from seawater and presumptively identified as Myroides sp., based on morphology, biochemical characteristics and 16S rDNA sequence. The strain produced surface-active compounds in marine broth, which were purified, using emulsification activity for n-hexadecane as an indicator. The purified compounds were identified by thin-layer chromatography, H-1- and C-13-NMR spectra and fast atom bombardment mass spectrometry as cholic acid, deoxycholic acid and their glycine conjugates. Type strains of the genus Myroides, M. odoratus JCM7458 and M. odoramitimus JCM7460, also produced these compounds. Myroides sp. strain SM1 possessed a biosynthetic route to cholic acid from cholesterol. Thus, bile acids were found as new products of prokaryotic cells, genus Myroides.

    DOI: 10.1007/s00253-004-1777-1

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  • 抗がん活性化合物の酵素合成

    神崎 浩

    ケミカルエンジニアリング50, 902-906   2005年

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  • Enzymatic synthesis of dehydro cyclo(His-Phe)s, analogs of the potent cell cycle inhibitor, dehydrophenylahistin, and their inhibitory activities toward cell division

    H Kanzaki, S Yanagisawa, T Nitoda

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY68 ( 11 ) 2341 - 2345   2004年11月

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    記述言語:英語   出版者・発行元:TAYLOR & FRANCIS LTD  

    Cyclo(His-Phe) was effectively converted to its dehydro, derivatives by the enzyme of Streptomyces albulus KO-23, an albonoursin-producing actinomycete. Two types of dehydro derivatives were isolated from the reaction mixture and identified as cyclo(DeltaHis-DeltaPhe) and cyclo(His-DeltaPhe). This is the first report on cyclo(His-DeltaPhe) and the enzymatic preparation of both compounds. Cyclo(DeltaHis-DeltaPhe), a tetradehydro cyclic dipeptide, exhibited a minimum inhibitory concentration of 0.78 mumol/ml inhibitory activity toward the first cleavage of sea urchin embryos, in contrast to cyclo(His-APhe) that had no activity. The finding that the isoprenylated derivative of cyclo(DeltaHis-DeltaPhe), dehydrophyenylahistin, had 2,000 times higher activity than cyclo(DeltaHis-DeltaPhe) indicates that an isoprenyl group attached to an imidazole ring of the compound was essential for the inhibitory activity.

    DOI: 10.1271/bbb.68.2341

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  • Monoacylglycerols: glycolipid biosurfactants produced by a thermotolerant yeast, Candida ishiwadae

    B Thanomsub, T Watcharachaipong, K Chotelersak, P Arunrattiyakorn, T Nitoda, H Kanzaki

    JOURNAL OF APPLIED MICROBIOLOGY96 ( 3 ) 588 - 592   2004年

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    記述言語:英語   出版者・発行元:WILEY  

    Aims: To isolate and characterize biosurfactants produced by a thermotolerant yeast isolated in Thailand.
    Materials and Results: Yeast strains isolated from plant material in Thailand were first screened for the ability to produce lipase and biosurfactant. A strain Y12, identified as Candida ishiwadae by physiological tests, survived at 45degreesC and produced relatively large amounts of biosurfactants. From the culture filtrate of this strain, two glycolipid biosurfactants, a and b, were purified by solvent fractionation, silica gel and ODS column chromatographies. Compounds a and b were determined to be monoacylglycerols; 1-linoleylglycerol and 1-oleylglycerol, respectively. Both compounds exhibited higher surfactant activities tested by the drop collapse test than several artificial surfactants such as sodium dodecyl sulphate.
    Conclusions: Glycolipid biosurfactants produced by a thermotolerant yeast, C. ishiwadae were characterized to be monoacylglycerols which exhibited high surfactant activities.
    Significance and Impact of the Study: A thermotolerant yeast strain, C. ishiwadae, could be a potential candidate for producing monoacylglycerols which are useful in industrial applications.

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  • A potent insect chitinase inhibitor of fungal origin

    T Nitoda, H Usuki, H Kanzaki

    ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES58 ( 11-12 ) 891 - 894   2003年11月

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    記述言語:英語   出版者・発行元:VERLAG Z NATURFORSCH  

    A water-soluble polysaccharide was isolated from the culture filtrate of a fungal strain, Sphaeropsis sp. TNPT116-Cz, as a novel insect chitinase inhibitor. It was purified to chromatographic homogeneity by ethanol precipitation, anion-exchange and gel filtration chromatography. Its molecular weight was estimated to be 16 kDa by gel filtration HPLC. Monosaccharide analysis showed that it contained glucose, galactose, N-acetylglucosamine and a deoxysugar. This polysaccharide showed potent and specific inhibitory activity against Spodoptera litura chitinase with an IC50 value of 28 nm.

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  • Augmentation by Bursaphelenchus xylophilus, a pine wood nematode, of polyclonal IgE production induced by lipopolysaccharide plus interleukin-4 in murine splenocytes

    H Kaji, M Kawada, A Tai, H Kanzaki, Yamamoto, I

    JOURNAL OF PHARMACOLOGICAL SCIENCES91 ( 2 ) 158 - 162   2003年2月

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    記述言語:英語   出版者・発行元:JAPANESE PHARMACOLOGICAL SOC  

    Bursaphelenchus xylophilus (B. xylophilus) is a pine wood nematode that is known to cause pine wilt disease. We report here that B. xylophilus extracts augmented the polyclonal immunoglobulin E (IgE) production induced by lipopolysaccharide (LPS) plus interleukin-4 (IL-4) both in murine splenocytes and purified B cells as determined by ELISA and ELIspot assays, but they did not cause such a promotion in the absence of either LPS or IL-4. We also observed that the antigen-nonspecific IgE levels were increased in sera of mice treated with B. xylophilus extracts, which were comparable to those of Ascaris suum extracts. These findings suggest that administration of B. xylophilus extracts could suppress allergic diseases via a saturation of mast cell Fcepsilon receptors or/and an inhibition of antigen-specific IgE synthesis to the allergen by a polyclonal response.

    DOI: 10.1254/jphs.91.158

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  • デヒドロアミノ酸残基を含む生理活性環状ジペプチド類

    神崎 浩

    生物工学会誌81, 396-399   2003年

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  • Effective production of potent cell cycle inhibitor dehydrophenylahistin by a combination of chemical racemization and Streptomyces enzyme-catalyzed conversion.

    Kanzaki, H, Ikeda, B, Nitoda, T

    Actinomycetologica   2003年

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  • Macromolecular insect chitinase inhibitors produced by fungi: Screening and partial characterization

    T Nitoda, H Usuki, A Kurata, H Kanzaki

    JOURNAL OF PESTICIDE SCIENCE28 ( 1 ) 33 - 36   2003年

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    記述言語:英語   出版者・発行元:PESTICIDE SCI SOC JAPAN  

    Culture broths of fungal strains were screened for novel insect chitinase inhibitors using the Spodoptera litura chitinase inhibitory assay. The culture filtrates of 5 strains showed potent and specific inhibitory activity against the insect chitinase. Partial characterization showed that the active compounds produced by these strains were water-soluble macromolecular compounds which had not been hitherto reported as chitinase inhibitors. These novel chitinase inhibitors are, therefore, expected to be potential agents for insect control.

    DOI: 10.1584/jpestics.28.33

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  • A novel potent cell cycle inhibitor dehydrophenylahistin - Enzymatic synthesis and inhibitory activity toward sea urchin embryo

    H Kanzaki, S Yanagisawa, K Kanoh, T Nitoda

    JOURNAL OF ANTIBIOTICS55 ( 12 ) 1042 - 1047   2002年12月

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    記述言語:英語   出版者・発行元:JAPAN ANTIBIOT RES ASSN  

    A novel dehydrogenated cyclic dipeptide named as dehydrophenylahistin (DeltaPLH) was effectively prepared from a fungal metabolite (-)-phenylahistin by the enzymatic conversion catalyzed by the cell-free extract of Streptomyces albulus KO-23, an albonoursin-producing actinomycete. DeltaPLH exhibited more than 1,000 times as high potent inhibitory activity toward the first cleavage of sea urchin embryos as (-)-phenylahisitn which has been reported to be a cell cycle inhibitor and more than 10,000 as high as albonoursin, indicating that DeltaPLH is a promising leading compound for anticancer drugs.

    DOI: 10.7164/antibiotics.55.1042

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  • 環状ジペプチド脱水素酵素で新規生理活性物質を作る

    神崎 浩

    バイオサイエンスとインダストリー60, 26-29   2002年

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  • 酵素による生理活性物質生産と情報化学の接点

    神崎 浩

    日本農芸化学会誌76, 539-541   2002年

  • 微生物由来のジケトピペラジン環化合物代謝酵素による生理活性物質生産

    神崎 浩

    生物工学会誌79(3), 71-77   2001年

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  • Biosynthetic intermediates of the tetradehydro cyclic dipeptide albonoursin produced by Streptomyces albulus KO-23

    H Kanzaki, S Yanagisawa, T Nitoda

    JOURNAL OF ANTIBIOTICS53 ( 11 ) 1257 - 1264   2000年11月

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    記述言語:英語   出版者・発行元:JAPAN ANTIBIOT RES ASSN  

    The cell-free extract of an albonoursin-producing strain Streptomyces albulus KO-23 catalyzes the conversion of cyclo(L-Leu-L-Phe) (1) to albonoursin (2). At the early stage of this conversion, two compounds were newly formed prior to albonoursin synthesis in the reaction mixture. These compounds were isolated and identified as (Z)-3-benzylidene-6-isobutyl-2,5-piperazinedione (4) and (Z)-3-benzyl-6-isobutylidene-2,5-piperazinedione (3). The cell-free extract also catalyzed the conversion of compound 3 or 4 to albonoursin. From these results, albonoursin was found to be biosynthesized via these compounds from cyclo(L-Leu-L-Phe). These didehydro diketopiperazines exhibited no inhibitory activity toward the first cleavage of sea urchin embryo in contrast to the higher cytotoxicity for albonoursin, indicating that dehydrogenation at alpha,beta -positions of both amino acid residues in diketopiperazines is required for cytotoxicity.

    DOI: 10.7164/antibiotics.53.1257

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  • Novel diketopiperazine metabolism in a microorganism: Two-step hydrolysis of cyclo(Gly-Leu) to amino acids and preliminary characterization of cyclo(Gly-Leu) hydrolase and dipeptidase

    H Kanzaki, N Mizuta, T Nitoda, K Kawazu

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING89 ( 6 ) 602 - 605   2000年6月

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    記述言語:英語   出版者・発行元:SOC BIOSCIENCE BIOENGINEERING JAPAN  

    A bacterium, strain NM 5-3, isolated from soil exhibited the highest cyclo(Gly-Leu) (CGL)-hydrolyzing activity and was identified as Agrobacterium radiobacter. The reaction products from CGL were dipeptides (Leu-Gly and Gly-Leu) and amino acids (Leu and Gly). Inhibitors for the dipeptidase of this strain did not inhibit the hydrolysis of CGL to dipeptides, indicating that two distinct enzymes, CGLase and a dipeptidase, were involved in its hydrolysis. The activities of these two enzymes were separated by anion-exchange column chromatography. The results indicated that strain NM5-3 hydrolyzed CGL via the dipeptides to the corresponding amino acids. The CGLase fraction was found to catalyze the hydrolysis of cyclo(Gly-D-Leu), cyclo(Gly-Gly), cyclo(L-Ala-Gly), and cyclo(D-Ala-Gly). On the other hand, the dipeptidase fraction exhibited L-specific substrate specificity.

    DOI: 10.1016/S1389-1723(00)80064-X

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  • Two antinematodal phenolics from Knema hookeriana, a Sumatran rainforest plant

    Y Alen, S Nakajima, T Nitoda, N Baba, H Kanzaki, K Kawazu

    ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES55 ( 3-4 ) 300 - 303   2000年3月

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    記述言語:英語   出版者・発行元:VERLAG Z NATURFORSCH  

    The activity-guided chromatographic purification of the methanol extract of Knema hookeriana, using pine wood nematodes Bursaphelenchus xylophilus has successfully led to the isolation and characterization of two phenolic antinematodal compounds with minimun effective dose (MED) of 4.5 and 20 mu g/cotton ball (mu g/ bl.) or 0.018 and 0.073 mu M/cotton ball (mu M/bl.), respectively. Based on their chemical and spectral properties, these compounds were determined to be 3-undecylphenol (1) and 3-(8Z-tridecenyl)-phenol (2). These compounds were isolated for the first time from this species, and 2 seems to be a novel compound.

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  • Antinematodal activity of some tropical rainforest plants against the pinewood nematode, Bursaphelenchus xylophilus

    Y Alen, S Nakajima, T Nitoda, N Baba, H Kanzaki, K Kawazu

    ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES55 ( 3-4 ) 295 - 299   2000年3月

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    記述言語:英語   出版者・発行元:VERLAG Z NATURFORSCH  

    Sixty five methanolic extracts of Sumatran rainforest plants representing 63 species of 21 families were assayed in vivo for antinematodal activity against Bursaphelenchus xylophilus using our cotton ball-fungal mat method. Extracts of 27 plants species from 14 families exhibited antinematodal activity, while 37 species were inactive. Among them, three extracts of Bischofia javanica, Knema hookeriana and Areca catechu exhibited very strong activity at minimum effective dose (MED) of 0.7 mg/cotton ball (mg/bl.). Eight extracts from Allamanda cathartica, Ervatamia corymbosa, Hoya diversifolia, Bischofia javanica, Derris malacensis, Melastoma melabathricum, Ophiorriza konsteleary and Brucea sumatrana also showed strong activity (MED, 5 mg/bl.).

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  • Effective production of dehydro cyclic dipeptide albonoursin exhibiting pronuclear fusion inhibitory activity II. Biosynthetic and bioconversion studies

    H Kanzaki, D Imura, T Nitoda, K Kawazu

    JOURNAL OF ANTIBIOTICS53 ( 1 ) 58 - 62   2000年1月

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    記述言語:英語   出版者・発行元:JAPAN ANTIBIOT RES ASSN  

    Albonoursin production was greatly enhanced when cycle (L-Leu-L-Phe) (CFL), a tetrahydro derivative of albonoursin, was added to the 2-day culture of an albonoursin-producing actinomycete, Streptomyces albulus KO-23. The increase in albonoursin production paralleled the amount of CFL added. Furthermore, the resting cells of the strain catalyzed the bioconversion of CFL to albonoursin. The optimum pH and temperature for the conversion were found to be pH 10.0 and 50 degrees C. The feeding experiments and the resting-cell reactions revealed that albonoursin is biosynthesized by dehydrogenation of CFL in the actinomycete. This is the first report for a dehydrogenation of amino acid residues at the alpha,beta-positions in cyclic dipeptides.

    DOI: 10.7164/antibiotics.53.58

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  • Effective production of dehydro cyclic dipeptide albonoursin exhibiting pronuclear fusion inhibitory activity II. Biosynthetic and bioconversion studies

    Hiroshi Kanzaki, Daisuke Imura, Teruhiko Nitoda, Kazuyoshi Kawazu

    Journal of Antibiotics53 ( 1 ) 58 - 62   2000年

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    記述言語:英語   出版者・発行元:Japan Antibiotics Research Association  

    Albornoursin production was greatly enhanced when cycle (r-Leu-L-Phe) (CFL), a tetrahydro derivative of albonoursin, was added to the 2-day culture of an albonoursin-producing actinomycete, Streptomyces albulus KO-23. The increase in albonoursin production paralleled the amount of CFL added. Furthermore, the resting cells of the strain catalyzed the bioconversion of CFL to albonoursin. The optimum pH and temperature for the conversion were found to be pH 10.0 and 50°C. The feeding experiments and the resting-cell reactions revealed that albonoursin is biosynthesized by dehydrogenation of CFL in the actinomycete. This is the first report for a dehydrogenation of amino acid residues at the α,β-positions in cyclic dipeptides.

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  • Enzymatic conversion of cyclic dipeptides to dehydro derivatives that inhibit cell division

    Hiroshi Kanzaki, Daisuke Imura, Teruhiko Nitoda, Kazuyoshi Kawazu

    Journal of Bioscience and Bioengineering90 ( 1 ) 86 - 89   2000年

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    記述言語:英語   出版者・発行元:Elsevier Science Publishers B.V.  

    The cell-free extract of an albonoursin-producing strain, Streptomyces albulus KO-23, was found to catalyze the conversion of several cyclic dipeptides having Phe and aliphatic side chain-containing amino acid residues to the corresponding dehydro derivatives. 3Z-Benzylidene-6S-methyl-2,5-piperazinedione, 3Z-benzylidene-2,5-piperazinedione, and 3Z, 6Z-dibenzylidene-2,5-piperazinedione were prepared by this conversion system. Among the dehydro cyclic dipeptides prepared, tetradehydro derivatives exhibited inhibitory activity toward the first cleavage of sea urchin embryo, while didehydro derivatives did not. We previously found that cyclo(Leu-Phe) and its didehydro derivatives did not show any inhibitory activity, in contrast to high activity in the case of albonoursin. Taken together, these findings indicate that dehydrogenation at the α, β-positions of both amino acid residues in this type of cyclic dipeptide is required for the inhibitory activity.

    DOI: 10.1016/S1389-1723(00)80039-0

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  • Effective production of dehydro cyclic dipeptide albonoursin exhibiting pronuclear fusion inhibitory activity II. Biosynthetic and bioconversion studies

    Hiroshi Kanzaki, Daisuke Imura, Teruhiko Nitoda, Kazuyoshi Kawazu

    Journal of Antibiotics53 ( 1 ) 58 - 62   2000年

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    記述言語:英語   出版者・発行元:Japan Antibiotics Research Association  

    Albornoursin production was greatly enhanced when cycle (r-Leu-L-Phe) (CFL), a tetrahydro derivative of albonoursin, was added to the 2-day culture of an albonoursin-producing actinomycete, Streptomyces albulus KO-23. The increase in albonoursin production paralleled the amount of CFL added. Furthermore, the resting cells of the strain catalyzed the bioconversion of CFL to albonoursin. The optimum pH and temperature for the conversion were found to be pH 10.0 and 50°C. The feeding experiments and the resting-cell reactions revealed that albonoursin is biosynthesized by dehydrogenation of CFL in the actinomycete. This is the first report for a dehydrogenation of amino acid residues at the α,β-positions in cyclic dipeptides.

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  • Effective production of dehydro cyclic dipeptide albonoursin exhibiting pronuclear fusion inhibitory activity I. Taxonomy and fermentation

    H Kanzaki, D Imura, R Sashida, A Kobayashi, K Kawazu

    JOURNAL OF ANTIBIOTICS52 ( 11 ) 1017 - 1022   1999年11月

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    記述言語:英語   出版者・発行元:JAPAN ANTIBIOT RES ASSN  

    Strain KO-23, an actinomycete producing albonoursin as well as streptopyrone, was identified as Streptomyces albulus by morphological and biochemical studies. Fermentation conditions for albonoursin, a dehydro cyclic dipeptide exhibiting a pronounced inhibitory activity toward pronuclear fusion of sea urchin eggs, were optimized. Under the optimum conditions, the actinomycete produced 16 mg/liter of albonoursin, 30 times higher than that in the original culture. The cells cultivated under these conditions highly express biosynthetic enzymes for albonoursin, and thus are available for biosynthetic studies of dehydro cyclic peptides.

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  • Inhibition of plant transformation by phytolaccoside B fron Phytolacca americana callus

    H Kanzaki, T Kagemori, Y Yamachika, T Nitoda, K Kawazu

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY63 ( 9 ) 1657 - 1659   1999年9月

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    記述言語:英語   出版者・発行元:TAYLOR & FRANCIS LTD  

    The newly established GUS expression bioassay on the callus extracts of 22 species of plants revealed that the methanol extract of Phytolacca americana callus had the most potent inhibitory activity against agrobacterial plant transformation. A triterpene glycoside phytolaccoside B was isolated from the extract as a genuine plant transformation inhibitor having neither antiagrobacterial nor phytotoxic activity. This compound is promising for use as a biochemical probe for studies on the plant transformation mechanism.

    DOI: 10.1271/bbb.63.1657

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  • Improved bioassay method for Spodoptera litura chitinase inhibitors using a colloidal chitin powder with a uniform particle size as substrate

    T Nitoda, H Kurumatani, H Kanzaki, K Kawazu

    PESTICIDE SCIENCE55 ( 5 ) 563 - 565   1999年5月

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    記述言語:英語   出版者・発行元:JOHN WILEY & SONS LTD  

    A previously reported bioassay method for Spodoptera litura chitinase inhibitors has been improved by use of colloidal chitin powder with a uniform particle size. This improvement made the assay four times more sensitive. Detection of three active supernatants by screening of supernatants and cell extracts from 135 fermentation broths has proved the efficiency of this improved method. (C) 1999 Society of Chemical Industry.

    DOI: 10.1002/(SICI)1096-9063(199905)55:5<563::AID-PS972>3.0.CO;2-#

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  • Enzymatic dehydrogenation of cyclo(L-Phe-L-Leu) to a bioactive derivative, albonoursin

    H Kanzaki, D Imura, T Nitoda, K Kawazu

    JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC6 ( 3 ) 265 - 270   1999年3月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    The cell-free extract of Streptomyces sp. KO-2388, an albonoursin-producing strain, was found to catalyze the conversion of cyclo(L-Phe-L-Leu) to albonoursin. The conversion activity was simply determined by measuring the increase in ultraviolet (UV) absorption of the reaction mixture at 317 nm, lambda(max) (epsilon 25,400) of albonoursin, where cyclo(L-Phe-L-Leu) had no absorption. The optimum pH and temperature of this bioconversion using the cell-free extract were determined to be pH 8.0-9.5 and 60 degrees C, respectively. Under the optimum conditions, 620 mg/l of albonoursin was obtained with a conversion ratio of 62% after 24 h incubation. (C) 1999 Elsevier Science B.V. All rights reserved.

    DOI: 10.1016/S1381-1177(98)00079-4

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  • Inhibition of plant transformation by phytolaccoside b from phytolacca americana callus

    Hiroshi Kanzaki, Toshihiko Kagemori, Yoko Yamachika, Teruhiko Nitoda, Kazuyoshi Kawazu

    Bioscience, Biotechnology and Biochemistry63 ( 9 ) 1657 - 1659   1999年1月

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    記述言語:英語  

    The newly established GUS expression bioassay on the callus extracts of 22 species of plants revealed that the methanol extract of Phytolacca americana callus had the most potent inhibitory activity against agrobacterial plant transformation. A triterpene glycoside phytolaccoside B was isolated from the extract as a genuine plant transformation inhibitor having neither antiagrobacterial nor phytotoxic activity. This compound is promising for use as a biochemical probe for studies on the plant transformation mechanism. © 1999, Taylor &amp
    Francis Group, LLC. All rights reserved.

    DOI: 10.1271/bbb.63.1657

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  • Inhibition of plant transformation by phytolaccoside b from phytolacca americana callus

    Hiroshi Kanzaki, Toshihiko Kagemori, Yoko Yamachika, Teruhiko Nitoda, Kazuyoshi Kawazu

    Bioscience, Biotechnology and Biochemistry63 ( 9 ) 1657 - 1659   1999年1月

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    記述言語:英語  

    The newly established GUS expression bioassay on the callus extracts of 22 species of plants revealed that the methanol extract of Phytolacca americana callus had the most potent inhibitory activity against agrobacterial plant transformation. A triterpene glycoside phytolaccoside B was isolated from the extract as a genuine plant transformation inhibitor having neither antiagrobacterial nor phytotoxic activity. This compound is promising for use as a biochemical probe for studies on the plant transformation mechanism. © 1999, Taylor &amp
    Francis Group, LLC. All rights reserved.

    DOI: 10.1271/bbb.63.1657

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  • 病原体を接種して萎凋させたアカマツ実生からの病原体の再分離およびマツノザイセンチュウの分離系統間の病原性の差異.

    河津一儀, 金子昇, 平岡享子, 山下秀昭, 神崎浩

    岡山大学農学部学術報告   1999年

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  • 新規の環状ジペプチド脱水素酵素とその活性測定法の確立.

    神崎浩, 赤澤和美, 井村大輔, 仁戸田照彦

    岡山大学農学部学術報告   1999年

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  • Effective production of dehydro cyclic dipeptide albonoursin exhibiting pronuclear fusion inhibitory activity. I. Taxonomy and fermentation

    Hiroshi Kanzaki, Daisuke Imura, Reiko Sashida, Akio Kobayashi, Kazuyoshi Kawazu

    Journal of Antibiotics52 ( 11 ) 1017 - 1022   1999年

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    記述言語:英語   出版者・発行元:Japan Antibiotics Research Association  

    Strain KO-23, an actinomycete producing albonoursin as well as streptopyrone, was identified as Streptomyces albulus by morphological and biochemical studies. Fermentation conditions for albonoursin, a dehydro cyclic dipeptide exhibiting a pronounced inhibitory activity toward pronuclear fusion of sea urchin eggs, were optimized. Under the optimum conditions, the actinomycete produced 16 mg/liter of albonoursin, 30 times higher than that in the original culture. The cells cultivated under these conditions highly express biosynthetic enzymes for albonoursin, and thus are available for biosynthetic studies of dehydro cyclic peptides.

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  • Effective production of dehydro cyclic dipeptide albonoursin exhibiting pronuclear fusion inhibitory activity. I. Taxonomy and fermentation

    Hiroshi Kanzaki, Daisuke Imura, Reiko Sashida, Akio Kobayashi, Kazuyoshi Kawazu

    Journal of Antibiotics52 ( 11 ) 1017 - 1022   1999年

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    記述言語:英語   出版者・発行元:Japan Antibiotics Research Association  

    Strain KO-23, an actinomycete producing albonoursin as well as streptopyrone, was identified as Streptomyces albulus by morphological and biochemical studies. Fermentation conditions for albonoursin, a dehydro cyclic dipeptide exhibiting a pronounced inhibitory activity toward pronuclear fusion of sea urchin eggs, were optimized. Under the optimum conditions, the actinomycete produced 16 mg/liter of albonoursin, 30 times higher than that in the original culture. The cells cultivated under these conditions highly express biosynthetic enzymes for albonoursin, and thus are available for biosynthetic studies of dehydro cyclic peptides.

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  • Improved bioassay method for plant transformation inhibitors

    Hiroshi Kanzaki, Toshihiko Kagemori, Satomi Asano, Kazuyoshi Kawazu

    Bioscience, Biotechnology and Biochemistry62 ( 12 ) 2328 - 2333   1998年

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    記述言語:英語  

    A convenient and quantitative bioassay method for evaluating the efficiency of plant transformation by Agrobacterium tumefaciens is important to search plant transformation inhibitors, possible biochemical probes for study on its mechanism. Our previously reported method, in which the plant transformation had been detected by the expression of β-glucuronidase in transformed plants, was improved. The difference between the previous and the improved methods is the use of suspension-cultured cells of Ageratum conyzoides as the host plant instead of Nicotiana tabacum BY-2
    this alteration of the host enabled us to measure the β-glucuronidase activity in plant cells not only fluorometrically but also colorimetrically. The enzyme activity expressed in the cells of A. conyzoides was nearly 100 times higher than that of N. tabacum BY-2, and was enough for detection by colorimetric measurement. The method, therefore, is useful for a convenient determination of inhibitory activity against plant transformation. © 1998, Taylor &amp
    Francis Group, LLC. All rights reserved.

    DOI: 10.1271/bbb.62.2328

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  • Improved bioassay method for plant transformation inhibitors

    Hiroshi Kanzaki, Toshihiko Kagemori, Satomi Asano, Kazuyoshi Kawazu

    Bioscience, Biotechnology and Biochemistry62 ( 12 ) 2328 - 2333   1998年

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    記述言語:英語  

    A convenient and quantitative bioassay method for evaluating the efficiency of plant transformation by Agrobacterium tumefaciens is important to search plant transformation inhibitors, possible biochemical probes for study on its mechanism. Our previously reported method, in which the plant transformation had been detected by the expression of β-glucuronidase in transformed plants, was improved. The difference between the previous and the improved methods is the use of suspension-cultured cells of Ageratum conyzoides as the host plant instead of Nicotiana tabacum BY-2
    this alteration of the host enabled us to measure the β-glucuronidase activity in plant cells not only fluorometrically but also colorimetrically. The enzyme activity expressed in the cells of A. conyzoides was nearly 100 times higher than that of N. tabacum BY-2, and was enough for detection by colorimetric measurement. The method, therefore, is useful for a convenient determination of inhibitory activity against plant transformation. © 1998, Taylor &amp
    Francis Group, LLC. All rights reserved.

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  • Novel bioactive oxazolomycin isomers produced by streptomyces albus JA3453

    Hiroshi Kanzaki, Ken-Ichi Wada, Teruhiko Nitoda, Kazuyoshi Kawazu

    Bioscience, Biotechnology and Biochemistry62 ( 3 ) 438 - 442   1998年

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    記述言語:英語  

    Two novel oxazolomycin isomers, oxazolomycins B (2) and C (3), were isolated from the fermentation broth of an oxazolomycin-producing strain, Streptomyces albus JA3453. Both compounds are geometrical isomers of oxazolomycin (1), the configurations of their triene moieties being (4′E, 6′E, 8′E) (2) and (4′Z, 6′E, 8′E) (3) while that of oxazolomycin (1) is (4′Z, 6′Z, 8′E). Compounds 2 and 3 exhibited potent inhibitory activity against crown gall formation with the same MIC (0.8 μg/disk) as oxazolomycin. Compounds 2 and 3 showed no antibacterial activity against Agrobacterium tumefaciens, in contrast to oxazolomycin which has specific anti-A. tumefaciens activity. © 1998, Taylor &amp
    Francis Group, LLC. All rights reserved.

    DOI: 10.1271/bbb.62.438

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  • Microbial hydrolysis of diketopiperazines: Different types of diketopiperazine-assimilating bacteria

    H Kanzaki, S Oda, A Kobayashi, K Kawazu

    JOURNAL OF FERMENTATION AND BIOENGINEERING83 ( 4 ) 386 - 388   1997年

     詳細を見る

    記述言語:英語   出版者・発行元:SOC FERMENTATION BIOENGINEERING, JAPAN  

    Arthrobacter sp. 1-3-1 and a coryneform rod bacterium, strain T-1-3-Y, which assimilate cyclo(Gly-Gly) and cyclo(Gly-L-Tyr) at high rates, respectively, were isolated from sell. Both bacteria exhibit diketopiperazine-hydrolyzing activity intracellularly in contrast to the extracellular cyclo(Gly-Gly) hydrolase reported previously. Arthrobacter sp. 1-3-1 shows a much broader substrate specificity than strain T-1-3-Y, indicating the diversity of diketopiperazine hydrolysis in nature.

    DOI: 10.1016/S0922-338X(97)80147-X

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  • Microbial hydrolysis of diketopiperazines: Different types of diketopiperazine-assimilating bacteria

    H Kanzaki, S Oda, A Kobayashi, K Kawazu

    JOURNAL OF FERMENTATION AND BIOENGINEERING83 ( 4 ) 386 - 388   1997年

     詳細を見る

    記述言語:英語   出版者・発行元:SOC FERMENTATION BIOENGINEERING, JAPAN  

    Arthrobacter sp. 1-3-1 and a coryneform rod bacterium, strain T-1-3-Y, which assimilate cyclo(Gly-Gly) and cyclo(Gly-L-Tyr) at high rates, respectively, were isolated from sell. Both bacteria exhibit diketopiperazine-hydrolyzing activity intracellularly in contrast to the extracellular cyclo(Gly-Gly) hydrolase reported previously. Arthrobacter sp. 1-3-1 shows a much broader substrate specificity than strain T-1-3-Y, indicating the diversity of diketopiperazine hydrolysis in nature.

    DOI: 10.1016/S0922-338X(97)80147-X

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  • Inhibitory effect of curromycins and their esters on plant transformation

    Hiroshi Kanzaki, Toshitsune Ichioka, Akio Kobayashi, Kazuyoshi Kawazu

    Bioscience, Biotechnology and Biochemistry60 ( 9 ) 1535 - 1537   1996年

     詳細を見る

    記述言語:英語  

    Curromycins A and B, antibiotics specific to Agrobacterium tumefaciens, inhibited crown gall formation on potato tubers. Their esters showed potent inhibitory activity against crown gall formation without any antibacterial activity. The change in biological activity of curromycins by simple esterification was similar to our previous observation in oxazolomycin. Curromycin esters are possible chemical probes to study the mechanism for plant transformation. © 1996, Taylor &amp
    Francis Group, LLC. All rights reserved.

    DOI: 10.1271/bbb.60.1535

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  • Inhibitory effect of curromycins and their esters on plant transformation

    Hiroshi Kanzaki, Toshitsune Ichioka, Akio Kobayashi, Kazuyoshi Kawazu

    Bioscience, Biotechnology and Biochemistry60 ( 9 ) 1535 - 1537   1996年

     詳細を見る

    記述言語:英語  

    Curromycins A and B, antibiotics specific to Agrobacterium tumefaciens, inhibited crown gall formation on potato tubers. Their esters showed potent inhibitory activity against crown gall formation without any antibacterial activity. The change in biological activity of curromycins by simple esterification was similar to our previous observation in oxazolomycin. Curromycin esters are possible chemical probes to study the mechanism for plant transformation. © 1996, Taylor &amp
    Francis Group, LLC. All rights reserved.

    DOI: 10.1271/bbb.60.1535

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  • An effective method of screening glucose-rich microbial culture filtrates for insect trehalase inhibitors

    Hiroshi Kanzaki, Takafumi Nuhama, Akio Kobayashi, Kazuyoshi Kawazu

    Bioscience, Biotechnology and Biochemistry59 ( 3 ) 398 - 400   1995年

     詳細を見る

    記述言語:英語  

    Trehalase inhibitors are promising as a specific insect growth regulator. Microbial culture filtrates are a good source of bioactive compounds, but some of them retain a significant amount of glucose, a major component of the culture medium, which makes it difficult to detect trehalase inhibitors because of high blank values for the glucose determination. To find a variety of trehalase inhibitors in glucose-rich microbial culture filtrates, an effective method for removing glucose by enzymatic conversion prior to a routine trehalase-inhibitory assay was devised. Conversion with glucose oxidase and catalase could completely remove 10mg/ml of glucose, and a wide variety of microbial culture filtrates with high glucose content could then be subjected to the routine bioassay. This method can also be applied to screen inhibitors for other enzymes whose activity is assayed by the amount of glucose formed. © 1995 Taylor &amp
    Francis Group LLC.

    DOI: 10.1080/bbb.59.398

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  • An effective method of screening glucose-rich microbial culture filtrates for insect trehalase inhibitors

    Hiroshi Kanzaki, Takafumi Nuhama, Akio Kobayashi, Kazuyoshi Kawazu

    Bioscience, Biotechnology and Biochemistry59 ( 3 ) 398 - 400   1995年

     詳細を見る

    記述言語:英語  

    Trehalase inhibitors are promising as a specific insect growth regulator. Microbial culture filtrates are a good source of bioactive compounds, but some of them retain a significant amount of glucose, a major component of the culture medium, which makes it difficult to detect trehalase inhibitors because of high blank values for the glucose determination. To find a variety of trehalase inhibitors in glucose-rich microbial culture filtrates, an effective method for removing glucose by enzymatic conversion prior to a routine trehalase-inhibitory assay was devised. Conversion with glucose oxidase and catalase could completely remove 10mg/ml of glucose, and a wide variety of microbial culture filtrates with high glucose content could then be subjected to the routine bioassay. This method can also be applied to screen inhibitors for other enzymes whose activity is assayed by the amount of glucose formed. © 1995 Taylor &amp
    Francis Group LLC.

    DOI: 10.1080/bbb.59.398

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  • Bisanthraquinones, Inhibitors of Plant Transformation

    Hiroshi Kanzaki, Goro Kawabata, Akio Kobayashi, Kazuyoshi Kawazu

    Bioscience, Biotechnology, and Biochemistry57 ( 12 ) 2104 - 2106   1993年

     詳細を見る

    記述言語:英語  

    A potato tuber disk assay of culture broths which had been confirmed to show neither antibacterial nor phytotoxic activity resulted in the discovery of a novel type of crown gall formation inhibitors, julimycin B-II and julichrome Q1.3. These two bisanthraquinones, products of Streptomyces sp. TM-71, inhibited crown gall formation on potato tuber disks at a minimum inhibitory dose of 1.6μg/disk without affecting the growth of Agrobacterium tumefaciens or the germination of alfalfa seeds. © 1993, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.

    DOI: 10.1271/bbb.57.2104

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  • Bisanthraquinones, Inhibitors of Plant Transformation

    Hiroshi Kanzaki, Goro Kawabata, Akio Kobayashi, Kazuyoshi Kawazu

    Bioscience, Biotechnology, and Biochemistry57 ( 12 ) 2104 - 2106   1993年

     詳細を見る

    記述言語:英語  

    A potato tuber disk assay of culture broths which had been confirmed to show neither antibacterial nor phytotoxic activity resulted in the discovery of a novel type of crown gall formation inhibitors, julimycin B-II and julichrome Q1.3. These two bisanthraquinones, products of Streptomyces sp. TM-71, inhibited crown gall formation on potato tuber disks at a minimum inhibitory dose of 1.6μg/disk without affecting the growth of Agrobacterium tumefaciens or the germination of alfalfa seeds. © 1993, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.

    DOI: 10.1271/bbb.57.2104

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  • Oxazolomycin Esters, Specific Inhibitors of Plant Transformation

    Kazuyoshi Kawazu, Goro Kawabata, Satoru Kawai, Akio Kobayashi, Hiroshi Kanzaki

    Bioscience, Biotechnology and Biochemistry56 ( 9 ) 1382 - 1385   1992年

     詳細を見る

    記述言語:英語  

    Oxazolomycin diacetate, dipropionate, monobutyrate and dibutyrate were derived from oxazolomycin, a product of Streptomyces sp. KBFP-2025. These esters were potent inhibitors of crown gall formation on potato tuber disks upon infection with Agrobacterium tumefaciens. They showed neither antibacterial nor phytotoxic activity, whereas oxazolomycin showed both antibacterial and phytotoxic activities. Further, they had no inhibitory activity against A. tumefaciens on the potato tuber disk. The inhibitory activity of these esters against crown gall formation seems to be due to specific inhibition of the transformation of plants with A. tumefaciens. © 1992, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.

    DOI: 10.1271/bbb.56.1382

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  • Production of Benzoylformic Acid from Phenylglycine by Saccharomycopsis lipolytica

    Hiroshi Kanzaki, Atsuhiko Isobe, Yoshikazu Izumi, Hideaki Yamada

    Agricultural and Biological Chemistry54 ( 8 ) 2101 - 2105   1990年

     詳細を見る

    記述言語:英語  

    Microbial production of benzoylformic acid (BF), which can be used as a substrate of enzymatic synthesis of (R)-(—)-mandelic acid, was investigated. Among 145 strains of yeasts and actinomycetes, Saccharomycopsis lipolytica (IAM 4964) was the best producer of BF from DL-phenylglycine (DL-PG). Culture conditions for BF production by the organism were optimized. When 0.2 % fructose as a carbon source and 0.7% Bacto-tryptone as a nitrogen source were used in the presence of 4 % dl-PG, 14.5 mg/ml of BF was produced (about 37 % molar yield) in 4 days of cultivation. BF was synthesized from the L-form of PG, but not from the D-form. The BF was isolated from culture broth in a crystalline form and physicochemically identified. © 1990, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.

    DOI: 10.1271/bbb1961.54.2101

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  • Production of Benzoylformic Acid from Phenylglycine by Saccharomycopsis lipolytica

    Hiroshi Kanzaki, Atsuhiko Isobe, Yoshikazu Izumi, Hideaki Yamada

    Agricultural and Biological Chemistry54 ( 8 ) 2101 - 2105   1990年

     詳細を見る

    記述言語:英語  

    Microbial production of benzoylformic acid (BF), which can be used as a substrate of enzymatic synthesis of (R)-(—)-mandelic acid, was investigated. Among 145 strains of yeasts and actinomycetes, Saccharomycopsis lipolytica (IAM 4964) was the best producer of BF from DL-phenylglycine (DL-PG). Culture conditions for BF production by the organism were optimized. When 0.2 % fructose as a carbon source and 0.7% Bacto-tryptone as a nitrogen source were used in the presence of 4 % dl-PG, 14.5 mg/ml of BF was produced (about 37 % molar yield) in 4 days of cultivation. BF was synthesized from the L-form of PG, but not from the D-form. The BF was isolated from culture broth in a crystalline form and physicochemically identified. © 1990, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.

    DOI: 10.1271/bbb1961.54.2101

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  • Characterization of crystalline formate dehydrogenase from Candida methanolica

    Yoshikazu IZUMI, Hiroshi KANZAKI, Shigeru MORITA, Hideaki FUTAZUKA, Hideaki YAMADA

    European Journal of Biochemistry182 ( 2 ) 333 - 341   1989年

     詳細を見る

    記述言語:英語  

    The crystalline formate dehydrogenase from Candida methanolica, which showed the highest specific activity (7.52 U/mg) so far reported, was characterized in detail. The enzyme is a dimer composed of identifical subunits, each containing one SH group related to the catalytic activity. The molecular mass of the enzyme is about 82–86 kDa. The Km values were found to be 3.0 mM for formate and 0.11 mM for NAD+. Even if the enzyme was incubated at pH 6.5–9.5 or at 55°C, the activity remained at 100%. Hg2+, Ni2+, NaCN, NaN3 and p‐chloromercuribenzoate strongly inhibited the enzyme activity, while the enzyme showed relatively high resistance to various chelating agents. The amino acid composition and some other physicochemical properties of the enzyme were studied. Immunological studies revealed that formate dehydrogenases of methanol‐utilizing yeasts immunologically more or less resemble each other, but differ from those of methanol‐utilizing bacteria. Furthermore, yeast formate dehydrogenases can be immunologically classified into three types: (a) the Candida type, (b) the Torulopsis/Hansenula/Pichia type and (c) the formaldehyde‐resistant yeast type. For simple and large‐scale preparation of the enzyme for practical use, treatment of cells of C. methanolica with the commercial cationic detergent, ‘Benzalkonium’ cation, is useful: the total and specific activities of the enzyme are 1.17‐fold and 3.10‐fold higher than those of the crude cell‐free extract, respectively. Copyright © 1989, Wiley Blackwell. All rights reserved

    DOI: 10.1111/j.1432-1033.1989.tb14835.x

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  • Characterization of crystalline formate dehydrogenase from Candida methanolica

    Yoshikazu IZUMI, Hiroshi KANZAKI, Shigeru MORITA, Hideaki FUTAZUKA, Hideaki YAMADA

    European Journal of Biochemistry182 ( 2 ) 333 - 341   1989年

     詳細を見る

    記述言語:英語  

    The crystalline formate dehydrogenase from Candida methanolica, which showed the highest specific activity (7.52 U/mg) so far reported, was characterized in detail. The enzyme is a dimer composed of identifical subunits, each containing one SH group related to the catalytic activity. The molecular mass of the enzyme is about 82–86 kDa. The Km values were found to be 3.0 mM for formate and 0.11 mM for NAD+. Even if the enzyme was incubated at pH 6.5–9.5 or at 55°C, the activity remained at 100%. Hg2+, Ni2+, NaCN, NaN3 and p‐chloromercuribenzoate strongly inhibited the enzyme activity, while the enzyme showed relatively high resistance to various chelating agents. The amino acid composition and some other physicochemical properties of the enzyme were studied. Immunological studies revealed that formate dehydrogenases of methanol‐utilizing yeasts immunologically more or less resemble each other, but differ from those of methanol‐utilizing bacteria. Furthermore, yeast formate dehydrogenases can be immunologically classified into three types: (a) the Candida type, (b) the Torulopsis/Hansenula/Pichia type and (c) the formaldehyde‐resistant yeast type. For simple and large‐scale preparation of the enzyme for practical use, treatment of cells of C. methanolica with the commercial cationic detergent, ‘Benzalkonium’ cation, is useful: the total and specific activities of the enzyme are 1.17‐fold and 3.10‐fold higher than those of the crude cell‐free extract, respectively. Copyright © 1989, Wiley Blackwell. All rights reserved

    DOI: 10.1111/j.1432-1033.1989.tb14835.x

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  • Purification and characterization of cystathionine γ‐synthase type II from Bacillus sphaericus

    Hiroshi KANZAKI, Michihiko KOBAYASHI, Toru NAGASAWA, Hideaki YAMADA

    European Journal of Biochemistry163 ( 1 ) 105 - 112   1987年

     詳細を見る

    記述言語:英語  

    Cystathionine γ‐synthase type II, which catalyzes l‐cystathionine synthesis from O‐acetyl‐l‐homoserine and l‐cysteine was purified from Bacillus sphaericus (IFO 3536) in seven steps. The purified enzyme appeared to be homogeneous by the results of polyacrylamide electrophoresis and ampholyte electrofocusing. The enzyme is a typical pyridoxal‐P dependent enzyme, has a molecular mass of 165 kDa and consists of four subunits identical in molecular mass. The enzyme catalyzed the γ‐replacement reaction and the elimination reaction was hardly detected even when a large amount of enzyme was added. In the replacement reaction, O‐acetyl‐l‐homoserine and the following thiol compounds: L and d‐cysteine, L and d‐homocysteine, sodium sulfide, various alkyl and aryl mercaptans, acted as the most suitable substrate to produce l‐cystathionine and the corresponding S‐substituted l‐homocysteine derivatives. Copyright © 1987, Wiley Blackwell. All rights reserved

    DOI: 10.1111/j.1432-1033.1987.tb10742.x

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  • Purification and Characterization of Cystathionine γ-Synthase Type II from #IDBBacillus(/)-#IRDB #IDBsphaericus(/)-#IRDB

    European Journal of Biochemistry163 ( 1 ) 105 - 112   1987年

     詳細を見る

  • Highly Efficient Production of L-Cystathionine from O#ID5-#IRD5-Succinyl-L-homoserine and L-Cysteine by #IDBStreptomyces(/)-#IRDB Cystathionine γ-Lyase

    Applied Microbiology and Biotechnology25 ( 2 ) 97 - 100   1986年

     詳細を見る

    記述言語:英語  

    DOI: 10.1007/BF00250516

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▼全件表示

講演・口頭発表等

  • 昆虫β-N-glucosaminidase特異的阻害剤HU-1の構成異常糖の合成と阻害活性評価

    日本農芸化学会2007年度中四国・西日本支部合同大会(中四国支部第19回講演会)  2007年 

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  • 強力な抗腫瘍活性物質へ変換可能なphenylahistinアナログ,aurantiamineの微生物生産と酵素変換

    日本農芸化学会2007年度大会  2007年 

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  • 大腸菌で発現させた放線菌由来Cyclo(Leu-Phe) oxidaseの精製と性質検討

    日本農芸化学会中四国支部第17回講演会  2007年 

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  • 微生物変換によるオリーブ成分の高機能化

    2007年 

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  • Verticillium sp. F40株が生産する新規 β-N-acetylglucosaminidase阻害剤

    日本農芸化学会2007年度大会  2007年 

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  • 微生物変換によるオリーブ二次代謝産物の高機能化

    日本農芸化学会中四国支部第18回講演会  2007年 

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  • Pochoina属糸状菌が生産する新規β-N-glucosaminidase阻害剤の阻害活性

    日本農芸化学会2007年度中四国・西日本支部合同大会(中四国支部第19回講演会)  2007年 

     詳細を見る

  • Enzymatic synthesis of bioactive dehydrogenated cyclic dipeptides by cyclo(Leu-Phe) oxidase from Streptomyces albulus KO23

    2005環太平洋国際化学会議 (PACIFICHEM 2005)  2005年 

     詳細を見る

  • Screening for cyclic dipeptide-producing microorganisms by cyclo(Leu-Phe) oxidase

    日本農芸化学会  2005年 

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  • 微生物酵素を利用するものづくり

    日本化学会中国四国支部岡山地区化学講演会  2005年 

     詳細を見る

  • 放線菌由来環状ジペプチド酸化酵素を利用するCyclo(His-His)脱水素体の合成

    日本農芸化学会  2005年 

     詳細を見る

  • フッ素アミノ酸添加培養による新規フッ素化アルボノルシンの微生物合成

    生物工学会大会  2005年 

     詳細を見る

  • Cyclo(Leu-Phe)酸化酵素が触媒するcyclo(Asp(OMe)-Asp(OMe))の脱水素反応

    日本農芸化学会関西・中四国・西日本支部合同支部大会  2005年 

     詳細を見る

  • Search for enzyme inhibitors specific for insect b-N-acetylglucosaminidase

    2005環太平洋国際化学会議 (PACIFICHEM 2005)  2005年 

     詳細を見る

  • 微生物酵素を利用するものづくり

    第3回 ニューバイオ技術研究交流会  2005年 

     詳細を見る

  • 微生物由来酸性多糖のアクチノリザル植物根粒窒素固定促進効果

    日本農芸化学会中四国支部例会  2005年 

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  • 昆虫のβ-N-acetylglucosaminidaseに特異的な阻害剤の探索(第3報)

    日本農芸化学会大会  2005年 

     詳細を見る

  • 抗がん活性化合物の酵素合成

    化学工学会秋季大会 シンポジウム 化学品製造をめざすバイオプロセス  2005年 

     詳細を見る

  • Novel Bioactive Dehydro Cyclic Dipeptides Prepared by Enzymatic Conversion

    国際微生物学会大会 (IUMS2005)  2005年 

     詳細を見る

  • Purification and characterization of L-homocysteine thiolactone hydrolase involved in dynamic resolution process of L-homocysteine.

    2005環太平洋国際化学会議 (PACIFICHEM 2005)  2005年 

     詳細を見る

  • Screening for cyclic dipeptide producing microorganisms by cyclo(Leu-Phe) oxidase

    2005環太平洋国際化学会議 (PACIFICHEM 2005)  2005年 

     詳細を見る

  • Production of a cell cycle inhibitor phenylahistin using membrane-surface liquid culture

    2005環太平洋国際化学会議 (PACIFICHEM 2005)  2005年 

     詳細を見る

  • Purification and charactarization of cyclo(Leu-Phe) oxidase from Streptomyces albulus KO23: An enzyme useful for bioactive dehydro cyclic dipeptide production

    2005環太平洋国際化学会議 (PACIFICHEM 2005)  2005年 

     詳細を見る

  • Effect of an acidic polysaccharide PS-1 on Frankia-actinorhizal plant nitrogen-fixing root nodule symbiosis

    2005環太平洋国際化学会議 (PACIFICHEM 2005)  2005年 

     詳細を見る

  • Production of 2-O-(b-D-glucopylanosyl)ascorbic acid by cultured cells of Lycium chinese

    2005環太平洋国際化学会議 (PACIFICHEM 2005)  2005年 

     詳細を見る

  • 膜面液体培養による細胞周期阻害剤Phenylahistinの生産

    平成16年度日本農芸化学会中四国支部大会  2004年 

     詳細を見る

  • 硝酸イオン資化性菌のスクリーニングと同定

    日本農芸化学会2004年度大会  2004年 

     詳細を見る

  • Albonoursin生合成系の素反応 cyclo (Leu-ΔPhe) からalbonoursinへの変換を触媒する酵素に関する検討

    日本農芸化学会2004年度大会  2004年 

     詳細を見る

  • Cyclo (Gly-Leu)資化性菌が生産するaminopeptidase

    日本農芸化学会2004年度大会  2004年 

     詳細を見る

  • 糸状菌TNPT116-Cz株が生産する昆虫キチナーゼ阻害多糖FPS-1の阻害特性

    日本農芸化学会2004年度大会  2004年 

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  • 昆虫のβ-N-acetylglucosaminidaseに特異的な阻害剤の探索

    日本農芸化学会2004年度大会  2004年 

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  • L-Homocysteine thiolactone加水分解酵素の精製と諸性質の検討 (第二報)

    平成16年度日本農芸化学会中四国支部大会  2004年 

     詳細を見る

  • 微生物由来酸性多糖のアクチノリザル植物根粒形成促進効果

    日本農芸化学会中四国支部第9回講演会  2004年 

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  • 生物資源からの高機能物質(生理活性物質)の探索と低機能物質(安価な基質)からの変換

    アグリビジネス創出産学官連携シンポジウム  2004年 

     詳細を見る

  • 細胞周期阻害剤dehydrophenylahistinのチオカルボニル化

    平成16年度日本生物工学会大会  2004年 

     詳細を見る

  • 糸状菌TNPT116-Cz株が生産する昆虫キチナーゼ阻害多糖FPS-1の阻害特性

    第8回生体触媒化学シンポジウム  2004年 

     詳細を見る

  • Agrobacterium radiobacter由来L-homocysteine thiolactone加水分解酵素の精製と性状解析

    第8回生体触媒化学シンポジウム  2004年 

     詳細を見る

  • 昆虫のb-N-acetylglucosaminidaseに特異的な阻害剤の探索(第2報)

    平成16年度日本農芸化学会中四国支部大会  2004年 

     詳細を見る

  • 糸状菌TNPT116-Cz株が生産する昆虫キチナーゼ阻害多糖FPS-1の阻害特性(第2報)

    平成16年度日本農芸化学会中四国支部大会  2004年 

     詳細を見る

  • 昆虫キチナーゼ阻害物質生産糸状菌由来のキチナーゼ

    日本農芸化学会中四国支部,西日本支部,日本栄養・食糧学会西日本支部, 日本食品科学工学会西日本支部 合同大会  2003年 

     詳細を見る

  • 糸状菌が生産する細胞分裂阻害物質 phenylahistin の生合成

    2003年度日本農芸化学会大会  2003年 

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  • 細胞周期阻害剤dehydrophenylahistinの酵素合成 −糸状菌培養物由来の立体異性混合物を基質とする効率的合成−

    日本生物工学会2003年度大会  2003年 

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  • L-Homocysteine thiolactone加水分解酵素の精製と諸性質の検討

    2003年度日本農芸化学会大会  2003年 

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  • Cyclo(Leu-Phe)からalbonoursinへの酵素変換反応の解析

    日本農芸化学会中四国支部,西日本支部,日本栄養・食糧学会西日本支部, 日本食品科学工学会西日本支部 合同大会  2003年 

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  • 放線菌由来cyclo (Leu-Phe)脱水素酵素の性状解析

    日本農芸化学会中四国支部,西日本支部,日本栄養・食糧学会西日本支部, 日本食品科学工学会西日本支部 合同大会  2003年 

     詳細を見る

  • 糸状菌が昆虫キチナーゼ阻害物質とともに生産するキチナーゼ

    第7回生体触媒化学シンポジウム  2003年 

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  • 放線菌由来cyclo (Leu-Phe)脱水素酵素の性状解析

    第7回生体触媒化学シンポジウム  2003年 

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  • 糸状菌HUF45株が生産する昆虫キチナーゼ阻害物質

    2002年度日本農芸化学会大会  2002年 

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  • 糸状菌TNPT116-Cz株が生産する昆虫キチナ-ゼ阻害活性を有する多糖

    2002年度日本農芸化学会大会  2002年 

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  • 強力な細胞分裂阻害活性を有するdehydrophenylahistinの化学-酵素合成

    2002年度日本農芸化学会大会  2002年 

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  • DL-Homocysteine thiolactoneからL-homocysteineへの微生物変換(第三報)

    2002年度日本農芸化学会大会  2002年 

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  • 糸状菌の生産する細胞分裂阻害物質(-)- phenylahistin の培養生産

    2002年度日本農芸化学会大会  2002年 

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  • 放線菌由来脱水素酵素系を用いた脱水素型環状ジペプチド類の合成

    第6回生体触媒化学シンポジウム  2002年 

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  • 放線菌由来cyclo (Leu-Phe)脱水素酵素の精製と諸性質の検討

    日本農芸化学会中四国支部大会  2002年 

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  • Agrobacterium属細菌の凝集促進活性を有する微生物細胞外多糖の性状解析

    日本農芸化学会中四国支部大会  2002年 

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  • 環状ジペプチド分解細菌が生産するジペプチダーゼの精製と諸性質の検討

    2002年度生物工学会大会  2002年 

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  • 放線菌由来環状ジペプチド脱水素酵素系を利用するcyclo(Phe-Pro)脱水素体の合成

    日本農芸化学会中四国支部大会  2002年 

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  • 放線菌由来環状ジペプチド脱水素酵素系を利用するCyclo (Met-Met)の脱水素体の合成

    日本農芸化学会2001年度関西・西日本・中四国支部合同大会  2001年 

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  • DL-ホモシステインチオラクトンからL-ホモシステインへの立体選択的微生物変換

    第4回生体触媒化学シンポジウム  2001年 

     詳細を見る

  • 生物変換による新規抗腫瘍活性化合物の生産

    バイオプロセスによる有機合成 岡山ミニシンポジウム(第4回)  2001年 

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  • 糸状菌の生産する昆虫キチナーゼ阻害物質

    日本農芸化学会2001年度大会  2001年 

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  • Novel Diketopiperazine Metabolism in a Microorganism

    18th International Congress on Heterocyclic Chemistry (第18回国際複素環化合物会議)  2001年 

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  • Production of Novel Bioactive Dehydro Cyclic Dipeptides By Microbial Enzyme System

    18th International Congress on Heterocyclic Chemistry (第18回国際複素環化合物会議)  2001年 

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  • 放線菌由来の新規環状ジペプチド脱水素酵素系を利用する有用物質生産(第二報)

    日本農芸化学会2001年度大会  2001年 

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  • Cyclo(Leu-Phe) からalbonoursin への脱水素反応を触媒する新規酵素系(第五報)

    日本農芸化学会2001年度大会  2001年 

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  • 脱水素型環状ジペプチド生合成酵素系による生理活性物質生産

    第43回天然有機化合物討論会  2001年 

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  • Production of Novel Bioactive Dehydro Cyclic Dipeptides By Microbial Enzyme System

    Biotrans 2001(The 5th International Symposium on Biocatalysis and Biotransformation)  2001年 

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  • 糸状菌由来昆虫キチナーゼ阻害物質の探索および性状比較

    日本農芸化学会2001年度関西・西日本・中四国支部合同大会  2001年 

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  • 酵素による生理活性物質生産と情報化学の接点

    藪田セミナー「IT時代のバイオサイエンス」  2001年 

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  • 糸状菌由来昆虫キチナーゼ阻害物質の探索

    第5回生体触媒化学シンポジウム  2001年 

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  • 生理活性を示す脱水素環状ジペプチド生産に有用な放線菌由来の新規脱水素酵素系

    第5回生体触媒化学シンポジウム  2001年 

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  • Agrobacterium tumefaciensの植物形質転換に関与する多糖

    日本農芸化学会2001年度関西・西日本・中四国支部合同大会  2001年 

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  • Cyclo(Gly-Leu)資化性菌由来Leucylglycine分解酵素

    第5回生体触媒化学シンポジウム  2001年 

     詳細を見る

  • Synthesis of bioactive dehydrodiketopiperazines catalyzed by a novel actinomycetous enzyme system

    Pacifichem 2000  2000年 

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  • cyclo(Leu-Phe) から albonoursin への脱水素反応を触媒する新規酵素系(第四報)

    日本農芸化学会2000年度大会  2000年 

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  • 放線菌の新規酵素系を用いた細胞分裂阻害活性を示す脱水素型環状ジペプチド類の合成

    日本農芸化学会2000年度大会  2000年 

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  • Agrobacterium属細菌の植物への付着を阻害する oxazolomycin diacetate の作用点

    日本農芸化学会2000年度大会  2000年 

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  • 放線菌新規酵素系を用いる生理活性脱水素型ジケトピペラジン類の合成

    第3回生体触媒化学シンポジウム  2000年 

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  • 糸状菌の生産する昆虫キチナーゼ阻害物質

    日本農芸化学会2000年度大会  2000年 

     詳細を見る

  • cyclo(Gly-Leu)資化性菌が生成するdipeptidase

    日本農芸化学会2000年度大会  2000年 

     詳細を見る

  • 微生物細胞外酸性多糖の植物形質転換に対する生理活性

    日本農芸化学会2000年度関西支部大会  2000年 

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  • 微生物由来のジケトピペラジン環化合物代謝酵素による生理活性物質生産

    日本生物工学会平成12年度大会  2000年 

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  • Production of Novel Bioactive Dehydro Cyclic Dipeptides by Streptomyces Enzyme System

    『耐熱性微生物の学際的研究ならびに資源開発とその応用』に関する拠点大学方式によるタイ国との共同研究の第2回合同セミナー  2000年 

     詳細を見る

  • 放線菌由来の新規環状ジペプチド脱水素酵素系を利用する有用物質生産

    日本農芸化学会2000年度関西支部大会  2000年 

     詳細を見る

  • 環状ジペプチド, cyclo (Gly-Leu) の微生物分解代謝に関与する新規酵素系

    日本生物工学会平成12年度大会  2000年 

     詳細を見る

  • Novel microbial metabolism of a morphine antagonist cyclo (Gly-Leu)

    Pacifichem 2000  2000年 

     詳細を見る

  • A Search for Insect Chitinase Inhibitors of Fungal Origin

    Pacifichem 2000  2000年 

     詳細を見る

  • Oxazolomycin diacetate, inhibitor against agrobacterial attachment to plants

    Pacifichem 2000  2000年 

     詳細を見る

  • Cyclo(Phe-Leu) からalbonoursin への脱水素反応を触媒する新規酵素系(第二報)

    日本農芸化学会1999年度大会  1999年 

     詳細を見る

  • Oxazolomycin ester の示すAgrobacterium 属細菌の植物への付着阻害活性

    日本農芸化学会1999年度大会  1999年 

     詳細を見る

  • DL-Homocysteine thiolactone から L-homocysteine への微生物変換(第二報)

    日本農芸化学会1999年度大会  1999年 

     詳細を見る

  • 微生物による cyclo(Gly-Leu) の分解

    日本生物工学会1999年度大会  1999年 

     詳細を見る

  • デヒドロアミノ酸を構成成分とするジケトピペラジン類の新規生合成系を利用する生理活性物質の酵素合成

    日本放線菌学会 1999年度大会  1999年 

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受賞

  • 日本生物工学会斎藤賞

    2000年  

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    受賞国:日本国

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  • 生理活性化学演習 (2020年度) 後期  - その他

  • 生理活性化学演習 (2020年度) 後期  - その他

  • 生理活性化学演習 (2020年度) 前期  - その他

  • 研究科目演習1 (2020年度) 特別  - その他

  • 研究科目演習2 (2020年度) 特別  - その他

  • 農学概論1 (2020年度) 第1学期  - 火3,火4

  • 農学概論2 (2020年度) 第2学期  - 火3,火4

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