論文 - 三谷　奈見季

Identification of the silicon form in xylem sap of rice (Oryza sativa L.)

N Mitani, JF Ma, T Iwashita

PLANT AND CELL PHYSIOLOGY   46 ( 2 )   279 - 283   2005年2月

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：OXFORD UNIV PRESS  

Rice (Oryza sativa L.) is a typical silicon (Si)-accumulating plant, but the mechanism responsible for the translocation from the root to the shoot is poorly understood. In this study, the form of Si in xylem sap was identified by 2 Si-29-nuclear magnetic resonance (NMR) spectroscopy. In rice (cv. Oochikara) cultured in a monosilicic acid solution containing 0.5 mM Si, the Si concentration in the xylem reached 6 mM,l within 30 min. In the Si-29-NMR spectra of the xylem sap, only one signal was observed at a chemical shift of -72.6 ppm, which is consistent with that of monosilicic acid. A H-1-NMR study of xylem sap did not show any significant difference between the wild-type rice and mutant rice defective in Si uptake, and the components of the xylem sap were not affected by the Si supply. The Si concentration in the xylem sap in vitro decreased from an initial 18 mM to 2.6 mM with time. Addition of xylem sap to a solution containing 8 mM Si did not prevent the polymerization of silicic acid. All these results indicate that Si is translocated in the form of monosilicic acid through the xylem and that the concentration of monosilicic acid is high in the xylem only transiently.

DOI： 10.1093/pcp/pci018

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Isolation and characterization of Lsi1 査読

Mitani N, Tamai K, Konishi S, Yano M, Yamaji N, Kyo M, Ma JF

Plant and Cell Physiology   46   S82   2005年

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