2021/04/08 更新

写真a

サトウ カズヒロ
佐藤 和広
SATO Kazuhiro
所属
資源植物科学研究所 教授
職名
教授
ホームページ
外部リンク

学位

  • 博士(農学) ( 北海道大学 )

研究キーワード

  • Plant Breeding

  • Genetic Resources

  • Genome Research

  • 植物育種

  • 遺伝資源

  • ゲノム研究

研究分野

  • 環境・農学 / 遺伝育種科学

学歴

  • 北海道大学   Faculty of Agriculture  

    - 1981年

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  • 北海道大学   農学部   農学科

    - 1981年

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    国名: 日本国

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経歴

  • - 岡山大学資源植物科学研究所 教授

    2009年

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  • - Professor,Institute of Plant Science and Resources,Okayama University

    2009年

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所属学協会

 

論文

  • The barley pan-genome reveals the hidden legacy of mutation breeding

    Murukarthick Jayakodi, Sudharsan Padmarasu, Georg Haberer, Venkata Suresh Bonthala, Heidrun Gundlach, Cécile Monat, Thomas Lux, Nadia Kamal, Daniel Lang, Axel Himmelbach, Jennifer Ens, Xiao-Qi Zhang, Tefera T. Angessa, Gaofeng Zhou, Cong Tan, Camilla Hill, Penghao Wang, Miriam Schreiber, Lori B. Boston, Christopher Plott, Jerry Jenkins, Yu Guo, Anne Fiebig, Hikmet Budak, Dongdong Xu, Jing Zhang, Chunchao Wang, Jane Grimwood, Jeremy Schmutz, Ganggang Guo, Guoping Zhang, Keiichi Mochida, Takashi Hirayama, Kazuhiro Sato, Kenneth J. Chalmers, Peter Langridge, Robbie Waugh, Curtis J. Pozniak, Uwe Scholz, Klaus F. X. Mayer, Manuel Spannagl, Chengdao Li, Martin Mascher, Nils Stein

    Nature   2020年11月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    <title>Abstract</title>Genetic diversity is key to crop improvement. Owing to pervasive genomic structural variation, a single reference genome assembly cannot capture the full complement of sequence diversity of a crop species (known as the ‘pan-genome’<sup>1</sup>). Multiple high-quality sequence assemblies are an indispensable component of a pan-genome infrastructure. Barley (<italic>Hordeum vulgare</italic> L.) is an important cereal crop with a long history of cultivation that is adapted to a wide range of agro-climatic conditions<sup>2</sup>. Here we report the construction of chromosome-scale sequence assemblies for the genotypes of 20 varieties of barley—comprising landraces, cultivars and a wild barley—that were selected as representatives of global barley diversity. We catalogued genomic presence/absence variants and explored the use of structural variants for quantitative genetic analysis through whole-genome shotgun sequencing of 300 gene bank accessions. We discovered abundant large inversion polymorphisms and analysed in detail two inversions that are frequently found in current elite barley germplasm; one is probably the product of mutation breeding and the other is tightly linked to a locus that is involved in the expansion of geographical range. This first-generation barley pan-genome makes previously hidden genetic variation accessible to genetic studies and breeding.

    DOI: 10.1038/s41586-020-2947-8

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    その他リンク: http://www.nature.com/articles/s41586-020-2947-8

  • Perspectives on Low Temperature Tolerance and Vernalization Sensitivity in Barley: Prospects for Facultative Growth Habit

    María Muñoz-Amatriaín, Javier Hernandez, Dustin Herb, P. Stephen Baenziger, Anne Marie Bochard, Flavio Capettini, Ana Casas, Alfonso Cuesta-Marcos, Claus Einfeldt, Scott Fisk, Amelie Genty, Laura Helgerson, Markus Herz, Gongshe Hu, Ernesto Igartua, Ildiko Karsai, Toshiki Nakamura, Kazuhiro Sato, Kevin Smith, Eric Stockinger, William Thomas, Patrick Hayes

    Frontiers in Plant Science11   2020年11月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Frontiers Media SA  

    One option to achieving greater resiliency for barley production in the face of climate change is to explore the potential of winter and facultative growth habits: for both types, low temperature tolerance (LTT) and vernalization sensitivity are key traits. Sensitivity to short-day photoperiod is a desirable attribute for facultative types. In order to broaden our understanding of the genetics of these phenotypes, we mapped quantitative trait loci (QTLs) and identified candidate genes using a genome-wide association studies (GWAS) panel composed of 882 barley accessions that was genotyped with the Illumina 9K single-nucleotide polymorphism (SNP) chip. Fifteen loci including 5 known and 10 novel QTL/genes were identified for LTT—assessed as winter survival in 10 field tests and mapped using a GWAS meta-analysis. <italic>FR-H1</italic>, <italic>FR-H2</italic>, and <italic>FR-H3</italic> were major drivers of LTT, and candidate genes were identified for <italic>FR-H3</italic>. The principal determinants of vernalization sensitivity were <italic>VRN-H1</italic>, <italic>VRN-H2</italic>, and <italic>PPD-H1</italic>. <italic>VRN-H2</italic> deletions conferred insensitive or intermediate sensitivity to vernalization. A subset of accessions with maximum LTT were identified as a resource for allele mining and further characterization. Facultative types comprised a small portion of the GWAS panel but may be useful for developing germplasm with this growth habit.

    DOI: 10.3389/fpls.2020.585927

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  • History and future perspectives of barley genomics

    Kazuhiro Sato

    DNA Research27 ( 4 )   2020年8月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

    <title>Abstract</title>
    Barley (Hordeum vulgare), one of the most widely cultivated cereal crops, possesses a large genome of 5.1 Gbp. Through various international collaborations, the genome has recently been sequenced and assembled at the chromosome-scale by exploiting available genetic and genomic resources. Many wild and cultivated barley accessions have been collected and preserved around the world. These accessions are crucial to obtain diverse natural and induced barley variants. The barley bioresource project aims to investigate the diversity of this crop based on purified seed and DNA samples of a large number of collected accessions. The long-term goal of this project is to analyse the genome sequences of major barley accessions worldwide. In view of technical limitations, a strategy has been employed to establish the exome structure of a selected number of accessions and to perform high-quality chromosome-scale assembly of the genomes of several major representative accessions. For the future project, an efficient annotation pipeline is essential for establishing the function of genomes and genes as well as for using this information for sequence-based digital barley breeding. In this article, the author reviews the existing barley resources along with their applications and discuss possible future directions of research in barley genomics.

    DOI: 10.1093/dnares/dsaa023

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    その他リンク: http://academic.oup.com/dnaresearch/article-pdf/27/4/dsaa023/34462420/dsaa023.pdf

  • Breeding for low cadmium barley by introgression of a Sukkula-like transposable element 査読

    Gui Jie Lei, Miho Fujii-Kashino, De Zhi Wu, Hiroshi Hisano, Daisuke Saisho, Fenglin Deng, Naoki Yamaji, Kazuhiro Sato, Fang-Jie Zhao, Jian Feng Ma

    Nature Food1 ( 8 ) 489 - 499   2020年8月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    DOI: 10.1038/s43016-020-0130-x

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    その他リンク: http://www.nature.com/articles/s43016-020-0130-x

  • Antagonistic regulation of the gibberellic acid response during stem growth in rice. 査読 国際誌

    Keisuke Nagai, Yoshinao Mori, Shin Ishikawa, Tomoyuki Furuta, Rico Gamuyao, Yoko Niimi, Tokunori Hobo, Moyuri Fukuda, Mikiko Kojima, Yumiko Takebayashi, Atsushi Fukushima, Yasuyo Himuro, Masatomo Kobayashi, Wataru Ackley, Hiroshi Hisano, Kazuhiro Sato, Aya Yoshida, Jianzhong Wu, Hitoshi Sakakibara, Yutaka Sato, Hiroyuki Tsuji, Takashi Akagi, Motoyuki Ashikari

    Nature584 ( 7819 ) 109 - 114   2020年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The size of plants is largely determined by growth of the stem. Stem elongation is stimulated by gibberellic acid1-3. Here we show that internode stem elongation in rice is regulated antagonistically by an 'accelerator' and a 'decelerator' in concert with gibberellic acid. Expression of a gene we name ACCELERATOR OF INTERNODE ELONGATION 1 (ACE1), which encodes a protein of unknown function, confers cells of the intercalary meristematic region with the competence for cell division, leading to internode elongation in the presence of gibberellic acid. By contrast, upregulation of DECELERATOR OF INTERNODE ELONGATION 1 (DEC1), which encodes a zinc-finger transcription factor, suppresses internode elongation, whereas downregulation of DEC1 allows internode elongation. We also show that the mechanism of internode elongation that is mediated by ACE1 and DEC1 is conserved in the Gramineae family. Furthermore, an analysis of genetic diversity suggests that mutations in ACE1 and DEC1 have historically contributed to the selection of shorter plants in domesticated populations of rice to increase their resistance to lodging, and of taller plants in wild species of rice for adaptation to growth in deep water. Our identification of these antagonistic regulatory factors enhances our understanding of the gibberellic acid response as an additional mechanism that regulates internode elongation and environmental fitness, beyond biosynthesis and gibberellic acid signal transduction.

    DOI: 10.1038/s41586-020-2501-8

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  • Accession difference in leaf photosynthesis, root hydraulic conductance and gene expression of root aquaporins under salt stress in barley seedlings 査読

    Asuka Kodama, Tamaki Watanabe, Makoto Yamaguchi, Ryohei Narita, Maki Katsuhara, Kazuhiro Sato, Taiichiro Ookawa, Tadashi Hirasawa

    Plant Production Science   1 - 10   2020年7月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Informa UK Limited  

    DOI: 10.1080/1343943x.2020.1794915

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  • East Asian origin of the widespread alpine snow‐bed herb, Primula cuneifolia (Primulaceae), in the northern Pacific region 査読

    Hajime Ikeda, Valentin Yakubov, Vyacheslav Barkalov, Kazuhiro Sato, Noriyuki Fujii

    Journal of Biogeography   2020年7月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/jbi.13918

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    その他リンク: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jbi.13918

  • Editorial: Use of Barley and Wheat Reference Sequences: Downstream Applications in Breeding, Gene Isolation, GWAS, and Evolution 査読

    Dragan Perovic, Hikmet Budak, Kazuhiro Sato, Pierre Sourdille

    Frontiers in Plant Science11   2020年7月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Frontiers Media SA  

    DOI: 10.3389/fpls.2020.01017

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  • Drastic shift in flowering phenology, an instant reproductive isolation mechanism, explains the population structure of Imperata cylindrica in Japan 査読

    Yasuyuki Nomura, Yoshiko Shimono, Nobuyuki Mizuno, Ikuya Miyoshi, Satoshi Iwakami, Kazuhiro Sato, Tohru Tominaga

        2020年7月

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    出版者・発行元:Cold Spring Harbor Laboratory  

    <title>Abstract</title>Reproductive isolation plays an important role in population differentiation and speciation, thus enhancing biodiversity in wild plants. Hybridisation sometimes involves rapid reproductive isolation between parents and their hybrids through the novel traits of hybrids derived from a new combination of genomes. Here, we report how a hybrids’ new phenotype contributes to rapid reproductive isolation between two ecotypes of <italic>Imperata cylindrica</italic>. The two ecotypes differ in their flowering phenology and habitats. An analysis with genetic markers revealed that hybrid populations consisted of only F<sub>1</sub> individuals. Both parental ecotypes flowered in spring, but F<sub>1</sub>s flowered in fall. This drastic shift in flowering phenology prevented backcrossing parental ecotypes to F<sub>1</sub>. F<sub>1</sub>s flowered in fall and dispersed seeds in winter. The germination percentage of seeds set on F<sub>1</sub> was extremely low in their habitats, and seedlings did not survive due to the low temperatures in winter, resulting in the absence of a F<sub>2</sub> generation. In conclusion, flowering phenology mismatch promotes reproductive isolation between parents and F<sub>1</sub>, resulting in a hybrid population consisting of only F<sub>1</sub>s.

    DOI: 10.1101/2020.06.30.179440

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  • Protocol for Genome Editing to Produce Multiple Mutants in Wheat 査読

    Fumitaka Abe, Yuji Ishida, Hiroshi Hisano, Masaki Endo, Toshihiko Komari, Seiichi Toki, Kazuhiro Sato

    STAR Protocols   100053 - 100053   2020年6月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.xpro.2020.100053

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  • Genetic Factors Associated with Heading Responses Revealed by Field Evaluation of 274 Barley Accessions for 20 Seasons 査読

    Kazuhiro Sato, Makoto Ishii, Kotaro Takahagi, Komaki Inoue, Minami Shimizu, Yukiko Uehara-Yamaguchi, Ryuei Nishii, Keiichi Mochida

    iScience23 ( 6 ) 101146 - 101146   2020年6月

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    担当区分:筆頭著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.isci.2020.101146

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  • Diploid genome differentiation conferred by RNA sequencing-based survey of genome-wide polymorphisms throughout homoeologous loci in Triticum and Aegilops. 査読 国際誌

    Sayaka Tanaka, Kentaro Yoshida, Kazuhiro Sato, Shigeo Takumi

    BMC genomics21 ( 1 ) 246 - 246   2020年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Triticum and Aegilops diploid species have morphological and genetic diversity and are crucial genetic resources for wheat breeding. According to the chromosomal pairing-affinity of these species, their genome nomenclatures have been defined. However, evaluations of genome differentiation based on genome-wide nucleotide variations are still limited, especially in the three genomes of the genus Aegilops: Ae. caudata L. (CC genome), Ae. comosa Sibth. et Sm. (MM genome), and Ae. uniaristata Vis. (NN genome). To reveal the genome differentiation of these diploid species, we first performed RNA-seq-based polymorphic analyses for C, M, and N genomes, and then expanded the analysis to include the 12 diploid species of Triticum and Aegilops. RESULTS: Genetic divergence of the exon regions throughout the entire chromosomes in the M and N genomes was larger than that between A- and Am-genomes. Ae. caudata had the second highest genetic diversity following Ae. speltoides, the putative B genome donor of common wheat. In the phylogenetic trees derived from the nuclear and chloroplast genome-wide polymorphism data, the C, D, M, N, U, and S genome species were connected with short internal branches, suggesting that these diploid species emerged during a relatively short period in the evolutionary process. The highly consistent nuclear and chloroplast phylogenetic topologies indicated that nuclear and chloroplast genomes of the diploid Triticum and Aegilops species coevolved after their diversification into each genome, accounting for most of the genome differentiation among the diploid species. CONCLUSIONS: RNA-sequencing-based analyses successfully evaluated genome differentiation among the diploid Triticum and Aegilops species and supported the chromosome-pairing-based genome nomenclature system, except for the position of Ae. speltoides. Phylogenomic and epigenetic analyses of intergenic and centromeric regions could be essential for clarifying the mechanisms behind this inconsistency.

    DOI: 10.1186/s12864-020-6664-3

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  • Identification of a Locus Conferring Dominant Susceptibility to Pyrenophora tritici-repentis in Barley 査読

    Bohan Wei, Matthew J. Moscou, Kazuhiro Sato, Ryan Gourlie, Stephen Strelkov, Reem Aboukhaddour

    Frontiers in Plant Science11   2020年2月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Frontiers Media SA  

    DOI: 10.3389/fpls.2020.00158

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  • Introgression of chromosomal segments conferring early heading date from wheat diploid progenitor, Aegilops tauschii Coss., into Japanese elite wheat cultivars. 査読 国際誌

    Shigeo Takumi, Seito Mitta, Shoya Komura, Tatsuya M Ikeda, Hitoshi Matsunaka, Kazuhiro Sato, Kentaro Yoshida, Koji Murai

    PloS one15 ( 1 ) e0228397   2020年

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    記述言語:英語  

    The breeding of agriculturally useful genes from wild crop relatives must take into account recent and future climate change. In Japan, the development of early heading wheat cultivars without the use of any major gene controlling the heading date is desired to avoid overlap of the harvesting time before the rainy season. Here, we backcrossed two early heading lines of a synthetic hexaploid wheat, derived from a crossing between durum wheat and the wild wheat progenitor Aegilops tauschii, with four Japanese elite cultivars to develop early heading lines of bread wheat. In total, nine early heading lines that showed a heading date two to eight days earlier than their parental cultivars in field conditions were selected and established from the selfed progenies of the two- or three-times backcrossed populations. The whole appearance and spike shape of the selected early heading lines looked like their parental wheat cultivars. The mature grains of the selected lines had the parental cultivars' characteristics, although the grains exhibited longer and narrower shapes. RNA sequencing-based genotyping was performed to detect single nucleotide polymorphisms between the selected lines and their parental wheat cultivars, which revealed the chromosomal regions transmitted from the parental synthetic wheat to the selected lines. The introgression regions could shorten wheat heading date, and their chromosomal positions were dependent on the backcrossed wheat cultivars. Therefore, early heading synthetic hexaploid wheat is useful for fine-tuning of the heading date through introgression of Ae. tauschii chromosomal regions.

    DOI: 10.1371/journal.pone.0228397

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  • Genome-wide polymorphisms from RNA sequencing assembly of leaf transcripts facilitate phylogenetic analysis and molecular marker development in wild einkorn wheat. 査読 国際誌

    Asami Michikawa, Kentaro Yoshida, Moeko Okada, Kazuhiro Sato, Shigeo Takumi

    Molecular genetics and genomics : MGG294 ( 5 ) 1327 - 1341   2019年10月

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    記述言語:英語  

    A survey of genome-wide polymorphisms between closely related species is required to understand the molecular basis of the evolutionary differentiation of their genomes. Two wild diploid wheat species, namely Triticum monococcum ssp. aegilopoides and T. urartu, are closely related and harbour the Am and A genomes, respectively. The A-genome donor of tetraploid and common wheat is T. urartu, and T. monococcum ssp. monococcum is the cultivated form derived from the wild einkorn wheat subspecies aegilopoides. Although subspecies aegilopoides has been a useful genetic resource in wheat breeding, genome-wide molecular markers for this subspecies have not been sufficiently developed. Here, we describe the detection of genome-wide polymorphisms such as single-nucleotide polymorphisms (SNPs) and insertions/deletions (indels) from RNA sequencing (RNA-seq) data of leaf transcripts in 15 accessions of the two diploid wheat species. The SNPs and indels, detected using the A genome of common wheat as the reference genome, covered the entire chromosomes of these species. The polymorphism information facilitated a comparison of the genetic diversity of einkorn wheat with that of two related diploid Aegilops species, namely, Ae. tauschii and Ae. umbellulata. Cleaved amplified polymorphic sequence (CAPS) markers converted from the SNP data were efficiently developed to confirm the addition of aegilopoides subspecies chromosomes to tetraploid wheat in nascent allohexaploid lines with AABBAmAm genomes. In addition, the CAPS markers permitted linkage map construction in mapping populations of aegilopoides subspecies accessions. Therefore, these RNA-seq data provide information for further breeding of closely related species with no reference genome sequence data.

    DOI: 10.1007/s00438-019-01581-9

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  • Genome-Edited Triple-Recessive Mutation Alters Seed Dormancy in Wheat. 査読 国際誌

    Fumitaka Abe, Emdadul Haque, Hiroshi Hisano, Tsuyoshi Tanaka, Yoko Kamiya, Masafumi Mikami, Kanako Kawaura, Masaki Endo, Kazumitsu Onishi, Takeshi Hayashi, Kazuhiro Sato

    Cell reports28 ( 5 ) 1362 - 1369   2019年7月

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    記述言語:英語  

    Common wheat has three sets of sub-genomes, making mutations difficult to observe, especially for traits controlled by recessive genes. Here, we produced hexaploid wheat lines with loss of function of homeoalleles of Qsd1, which controls seed dormancy in barley, by Agrobacterium-mediated CRISPR/Cas9. Of the eight transformed wheat events produced, three independent events carrying multiple mutations in wheat Qsd1 homeoalleles were obtained. Notably, one line had mutations in every homeoallele. We crossed this plant with wild-type cultivar Fielder to generate a transgene-free triple-recessive mutant, as revealed by Mendelian segregation. The mutant showed a significantly longer seed dormancy period than wild-type, which may result in reduced pre-harvest sprouting of grains on spikes. PCR, southern blotting, and whole-genome shotgun sequencing revealed that this segregant lacked transgenes in its genomic sequence. This technique serves as a model for trait improvement in wheat, particularly for genetically recessive traits, based on locus information from diploid barley.

    DOI: 10.1016/j.celrep.2019.06.090

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  • Origin of wheat B-genome chromosomes inferred from RNA sequencing analysis of leaf transcripts from section Sitopsis species of Aegilops. 査読 国際誌

    Yuka Miki, Kentaro Yoshida, Nobuyuki Mizuno, Shuhei Nasuda, Kazuhiro Sato, Shigeo Takumi

    DNA research : an international journal for rapid publication of reports on genes and genomes26 ( 2 ) 171 - 182   2019年4月

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    記述言語:英語  

    Dramatic changes occasionally occur in intergenic regions leading to genomic alterations during speciation and will consequently obscure the ancestral species that have contributed to the formation of allopolyploid organisms. The S genome of five species of section Sitopsis of genus Aegilops is considered to be an origin of B-genome in cultivated tetraploid and hexaploid wheat species, although its actual donor is still unclear. Here, we attempted to elucidate phylogenetic relationship among Sitopsis species by performing RNA sequencing of the coding regions of each chromosome. Thus, genome-wide polymorphisms were extensively analyzed in 19 accessions of the Sitopsis species in reference to the tetraploid and hexaploid wheat B genome sequences and consequently were efficiently anchored to the B-genome chromosomes. The results of our genome-wide exon sequencing and resultant phylogenetic analysis indicate that Ae. speltoides is likely to be the direct donor of all chromosomes of the wheat B genome. Our results also indicate that the genome differentiation during wheat allopolyploidization from S to B proceeds at different speeds over the chromosomes rather than at constant rate and recombination could be a factor determining the speed. This observation is potentially generalized to genome differentiation during plant allopolyploid evolution.

    DOI: 10.1093/dnares/dsy047

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  • Agrobacterium tumefaciens enhances biosynthesis of two distinct auxins in the formation of crown galls. 査読

    Mashiguchi, K, Hisano, H, Takeda-Kamiya, N, Takebayashi, Y, Ariizumi, T, Gao, Y, Ezura, H, Sato, K, Zhao, Y, Hayashi, K, Kasahara, H

    Plant and Cell Physiology60 ( 1 ) 29 - 37   2019年1月

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    記述言語:英語  

    DOI: 10.1093/pcp/pcy182

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  • Development of Genome-Wide SNP Markers for Barley via Reference- Based RNA-Seq Analysis. 査読 国際誌

    Tsuyoshi Tanaka, Goro Ishikawa, Eri Ogiso-Tanaka, Takashi Yanagisawa, Kazuhiro Sato

    Frontiers in plant science10   577 - 577   2019年

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    記述言語:英語  

    Marker-assisted selection of crop plants requires DNA markers that can distinguish between the closely related strains often used in breeding. The availability of reference genome sequence facilitates the generation of markers, by elucidating the genomic positions of new markers as well as of their neighboring sequences. In 2017, a high quality genome sequence was released for the six-row barley (Hordeum vulgare) cultivar Morex. Here, we developed a de novo RNA-Seq-based genotyping procedure for barley strains used in Japanese breeding programs. Using RNA samples from the seedling shoot, seedling root, and immature flower spike, we mapped next-generation sequencing reads onto the transcribed regions, which correspond to ∼590 Mb of the whole ∼4.8-Gbp reference genome sequence. Using 150 samples from 108 strains, we detected 181,567 SNPs and 45,135 indels located in the 28,939 transcribed regions distributed throughout the Morex genome. We evaluated the quality of this polymorphism detection approach by analyzing 387 RNA-Seq-derived SNPs using amplicon sequencing. More than 85% of the RNA-Seq SNPs were validated using the highly redundant reads from the amplicon sequencing, although half of the indels and multiple-allele loci showed different polymorphisms between the platforms. These results demonstrated that our RNA-Seq-based de novo polymorphism detection system generates genome-wide markers, even in the closely related barley genotypes used in breeding programs.

    DOI: 10.3389/fpls.2019.00577

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  • QTLs maintaining grain fertility under salt stress detected by exome QTL-seq and interval mapping in barley. 査読

    Asuka Kodama, Ryouhei Narita, Makoto Yamaguchi, Hiroshi Hisano, Shunsuke Adachi, Hiroki Takagi, Taiichiro Ookawa, Kazuhiro Sato, Tadashi Hirasawa

    Breeding science68 ( 5 ) 561 - 570   2018年12月

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    記述言語:英語  

    Enhancing salt stress tolerance is a key strategy for increasing global food production. We previously found that long-term salinity stress significantly reduced grain fertility in the salt-sensitive barley (Hordeum vulgare) accession, 'OUC613', but not in the salt-tolerant accession, 'OUE812', resulting in large differences in grain yield. Here, we examined the underlying causes of the difference in grain fertility between these accessions under long-term treatment with 150 or 200 mM NaCl from the seedling stage to harvest and identified quantitative trait loci (QTLs) for maintaining grain fertility. In an artificial pollination experiment of the two accessions, grain fertility was significantly reduced only in OUC613 plants produced using pollen from plants grown under NaCl stress, suggesting that the low grain fertility of OUC613 was mainly due to reduced pollen fertility. Using QTL-seq combined with exome-capture sequencing and composite interval mapping of recombinant inbred lines derived from a cross between OUE812 and OUC613, we identified a QTL (qRP-2Hb) for grain fertility on chromosome 2H. The QTL region includes two genes encoding an F-box protein and a TIFY protein that are associated with male sterility, highlighting the importance of this region for maintaining grain fertility under salt stress.

    DOI: 10.1270/jsbbs.18082

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  • RNA Sequencing-Based Bulked Segregant Analysis Facilitates Efficient D-genome Marker Development for a Specific Chromosomal Region of Synthetic Hexaploid Wheat. 査読 国際誌

    Ryo Nishijima, Kentaro Yoshida, Kohei Sakaguchi, Shin-Ichi Yoshimura, Kazuhiro Sato, Shigeo Takumi

    International journal of molecular sciences19 ( 12 )   2018年11月

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    記述言語:英語  

    Common wheat originated from interspecific hybridization between cultivated tetraploid wheat and its wild diploid relative Aegilops tauschii followed by amphidiploidization. This evolutionary process can be reproduced artificially, resulting in synthetic hexaploid wheat lines. Here we performed RNA sequencing (RNA-seq)-based bulked segregant analysis (BSA) using a bi-parental mapping population of two synthetic hexaploid wheat lines that shared identical A and B genomes but included with D-genomes of distinct origins. This analysis permitted identification of D-genome-specific polymorphisms around the Net2 gene, a causative locus to hybrid necrosis. The resulting single nucleotide polymorphisms (SNPs) were classified into homoeologous polymorphisms and D-genome allelic variations, based on the RNA-seq results of a parental tetraploid and two Ae. tauschii accessions. The difference in allele frequency at the D-genome-specific SNP sites between the contrasting bulks (ΔSNP-index) was higher on the target chromosome than on the other chromosomes. Several SNPs with the highest ΔSNP-indices were converted into molecular markers and assigned to the Net2 chromosomal region. These results indicated that RNA-seq-based BSA can be applied efficiently to a synthetic hexaploid wheat population to permit molecular marker development in a specific chromosomal region of the D genome.

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  • RNA-seq analysis reveals considerable genetic diversity and provides genetic markers saturating all chromosomes in the diploid wild wheat relative Aegilops umbellulata. 査読 国際誌

    Moeko Okada, Kentaro Yoshida, Ryo Nishijima, Asami Michikawa, Yuka Motoi, Kazuhiro Sato, Shigeo Takumi

    BMC plant biology18 ( 1 ) 271 - 271   2018年11月

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    記述言語:英語  

    BACKGROUND: Aegilops umbellulata Zhuk. (2n = 14), a wild diploid wheat relative, has been the source of trait improvement in wheat breeding. Intraspecific genetic variation of Ae. umbellulata, however, has not been well studied and the genomic information in this species is limited. RESULTS: To develop novel genetic markers distributed over all chromosomes of Ae. umbellulata and to evaluate its genetic diversity, we performed RNA sequencing of 12 representative accessions and reconstructed transcripts by de novo assembly of reads for each accession. A large number of single nucleotide polymorphisms (SNPs) and insertions/deletions (indels) were obtained and anchored to the pseudomolecules of Ae. tauschii and barley (Hordeum vulgare L.), which were regarded as virtual chromosomes of Ae. umbellulata. Interestingly, genetic diversity in Ae. umbellulata was higher than in Ae. tauschii, despite the narrow habitat of Ae. umbellulata. Comparative analyses of nucleotide polymorphisms between Ae. umbellulata and Ae. tauschii revealed no clear lineage differentiation and existence of alleles with rarer frequencies predominantly in Ae. umbellulata, with patterns clearly distinct from those in Ae. tauschii. CONCLUSIONS: The anchored SNPs, covering all chromosomes, provide sufficient genetic markers between Ae. umbellulata accessions. The alleles with rarer frequencies might be the main source of the high genetic diversity in Ae. umbellulata.

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  • Retrotransposon Insertion and DNA Methylation Regulate Aluminum Tolerance in European Barley Accessions. 査読 国際誌

    Miho Kashino-Fujii, Kengo Yokosho, Naoki Yamaji, Miki Yamane, Daisuke Saisho, Kazuhiro Sato, Jian Feng Ma

    Plant physiology178 ( 2 ) 716 - 727   2018年10月

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    記述言語:英語  

    Aluminum (Al) toxicity is a major stress factor limiting crop productivity in acid soil. Although there is great genotypic variation in tolerance to Al toxicity, the underlying molecular mechanisms are poorly understood. Here, we report that, in barley (Hordeum vulgare), the fourth largest cereal crop produced in the world, both retrotransposon insertion and DNA methylation are involved in regulating differential Al tolerance. HvAACT1 is a major gene responsible for citrate secretion from the roots for external detoxification of Al. A multiretrotransposon-like (MRL) sequence insertion at least 15.3 kb in length was detected in the upstream genomic region of HvAACT1 that displayed promoter activity and significantly enhanced HvAACT1 expression, especially in the root tips of Al-tolerant accessions. Furthermore, in a number of accessions with low levels of HvAACT1 expression, this MRL insertion was present but highly methylated. Geographical analysis showed that accessions with this MRL insertion are distributed mainly in European areas with acid soils. Two wild barley accessions were found to possess this MRL insertion, but with a high degree of methylation. These results indicate that the MRL insertion and its degree of DNA methylation influence HvAACT1 expression and that demethylation of this MRL insertion, which facilitates adaptation to acid soils, occurred following barley domestication. Moreover, our results indicate that barley accessions in East Asia and Europe have developed independent but equivalent strategies to withstand Al toxicity in acid soils.

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  • A single nucleotide polymorphism in the “Fra” gene results in fractured starch granules in barley. 査読 国際誌

    Saito M, Tanaka, T, Sato, K, Vrinten, P, Nakamura, T

    Theoretical and Applied Genetics131 ( 2 ) 353 - 364   2018年2月

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  • Detection of QTLs controlling alpha-amylase activity in a diversity panel of 343 barley accessions

    Kazuhiro Sato, Hiroshi Hisano, Satoko Matsumoto, Tian-Su Zhou, Makoto Kihara

    Molecular Breeding38 ( 1 ) 14   2018年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Netherlands  

    The α–amylase activity of cultivated barley is critically important to the brewing industry. Here, we surveyed variation in malt α–amylase activity in 343 cultivated barley accessions from around the world. Population structure analysis based on genotype data at 1536 SNPs clustered these accessions into two groups, one comprising South-East Asian and Ethiopian accessions and one group containing the other accessions. A genome-wide association study identified significant quantitative trait loci (QTLs) for α–amylase activity on all seven chromosomes of barley. Accessions showing high and low α–amylase activity were crossed with the high-quality Japanese malting barley cv. Harun Nijo to develop F2 mapping populations. We identified two QTLs on chromosome 6H in a cross between Haruna Nijo (high activity) × Weal (highest activity). Single QTLs were identified each on 3H, 4H, and 5H from a cross between Haruna Nijo (high activity) × VLB-1 (low activity), indicating that the high α–amylase activity in Haruna Nijo might be derived from loci on these chromosomes. The addition of the high α–amylase activity QTL alleles from chromosome 6H in cv. Weal further increased the α–amylase activity conferred by alleles of Haruna Nijo. These results demonstrate that a target haplotype can be successfully improved using a strategy comprising diversity analysis of ex situ collections followed by introducing effective new alleles.

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  • Genotypic Difference in Grain Fertility under Salt Stress and the Responsible Quantitative Trait Loci in Barley.

    Kodama, A, Narita, R, Yamaguchi, M, Hisano, H, Adachi, S, Takagi, H, Ookawa, T, Sato, K, Hirasawa, T

    Breeding Science   2018年

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  • Marker-trait associations in two-rowed spring barley accessions from Kazakhstan and the USA. 査読 国際誌

    Yuliya Genievskaya, Shyryn Almerekova, Burabai Sariev, Vladimir Chudinov, Laura Tokhetova, Grigoriy Sereda, Anarbai Ortaev, Vladimir Tsygankov, Thomas Blake, Shiaoman Chao, Kazuhiro Sato, Saule Abugalieva, Yerlan Turuspekov

    PloS one13 ( 10 ) e0205421   2018年

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    記述言語:英語  

    In this study, phenotyping and single nucleotide polymorphism (SNP) genotyping data of 272 accessions of two-rowed spring barley from the USA along with 94 accessions from Kazakhstan were assessed in field trials at six breeding organizations in Kazakhstan to evaluate the performance of the USA samples over three years (2009-2011). The average grain yield over the six locations was not significantly higher in Kazakh accessions in comparison to the USA samples. Twenty four samples from Montana, Washington, the USDA station in Aberdeen Idaho, and the Anheuser-Busch breeding programs showed heavier average yield than the local standard cultivar "Ubagan". Principal Coordinate analysis based on two sets of SNP data suggested that Kazakh accessions were closest to the USA accessions among eight groups of samples from different parts of the World, and within five US barley origin groups the samples from Montana and Washington perfectly matched six groups of Kazakh breeding origins. A genome-wide association study (GWAS) using data from eighteen field trials allowed the identification of ninety one marker-trait associations (MTA) in two or more environments for nine traits, including key characters such as heading time (HT), number of kernels per spike (NKS), and thousand grain weight (TGW). Our GWAS allowed the identification of eight MTA for HT and NKS, and sixteen MTA for TGW, when those MTA were linked to mapped SNPs. Based on comparisons of chromosomal positions of MTA identified in this study, and positions of known genes and quantitative trait loci for HT, NKS and TGW, it was suggested that MTA for HT on chromosome 2H (at 158.2 cM, 11_21414), MTA for NKS on 5H (at 118.6 cM, 11_20298), and two MTA for TGW on chromosome 4H (at 94.7 cM, 12_30718, and at 129.3 cM, 11_20013) were potentially new associations in barley. GWAS suggested that six MTA for HT, including two on chromosome 1H, two on chromosome 3H, and one each on chromosomes 4H and 6H, had useful pleiotropic effects for improving barley spike traits.

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  • Expression of Barley Glutathione S-Transferase13 Gene Reduces Accumulation of Reactive Oxygen Species by Trichothecenes and Paraquat in Arabidopsis Plants. 査読

    Wahibah, N, Tsutsui, T, Tamaoki, D, Sato, K, Nishiuchi, T

    Plant Biotechnology35 ( 1 ) 71 - 79   2018年

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  • Nicotinamide mononucleotide and related metabolites induce the disease resistance against the fungal phytopathogen, Fusarium graminearum in Arabidopsis thaliana and Hordeum vulgare. 査読 国際誌

    Miwa, Akihiro, Sawada, Yuji, Tamaoki, Daisuke, Hirai, Masami, Kimura, Makoto, Sato, Kazuhiro, Nishiuchi, Takumi

    Scientific Reports7 ( 1 ) 6389 - 6389   2017年7月

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  • Sequence differences in the seed dormancy gene Qsd1 among various wheat genomes 査読 国際誌

    Kazumitsu Onishi, Miki Yamane, Nami Yamaji, Mayumi Tokui, Hiroyuki Kanamori, Jianzhong Wu, Takao Komatsuda, Kazuhiro Sato

    BMC Genomics18 ( 1 ) 497 - 497   2017年6月

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  • Selection of transformation-efficient barley genotypes based on TFA (transformation amenability) haplotype and higher resolution mapping of the TFA loci 査読 国際誌

    Hiroshi Hisano, Brigid Meints, Matthew, J. Moscou, Luis Cistue, Bego?a Ech?varri, Kazuhiro Sato, Patrick M. Hayes

    Plant Cell Reports36 ( 4 ) 611 - 620   2017年4月

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  • Detection of splicing variants in the leaf and spike transcripts of wild diploid wheat Aegilops tauschii and transmission of the splicing patterns to synthetic hexaploid wheat

    Julio C.M. Iehisa, Moeko Okada, Kazuhiro Sato, Shigeo Takumi

    Plant Gene9   6 - 12   2017年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier B.V.  

    Alternative splicing produces multiple transcripts from a single gene, and RNA sequencing using next-generation sequencing apparatus has contributed to comprehensive detection of splicing variants in higher plants. Here, we completed a large-scale survey of alternatively spliced transcripts from RNA samples derived from seedling leaves and young spikes of two distinct accessions of wild diploid wheat Aegilops tauschii under normal growth conditions. Alternative splicing for 23,778 loci were analyzed in this study, and at least two splicing variants were found in 4712 genes. The proportion of events for intron retention was much smaller in Ae. tauschii than in other plant species, whereas the proportions of exon skipping and splice donor site events were higher. In at least some genes, alternative splicing patterns were clearly distinct between the two Ae. tauschii accessions. Distinct splicing patterns were transmitted from the parental Ae. tauschii accessions into their synthetic hexaploid wheat lines. Alternative splicing patterns in one of the two synthetic hexaploid wheat lines analyzed appeared to be generally similar to patterns intermediate between the parental lines under normal growth conditions, and seemed to be slightly altered in the other synthetic wheat.

    DOI: 10.1016/j.plgene.2016.11.002

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  • Exome QTL-seq maps monogenic locus and QTLs in barley 査読 国際誌

    Hiroshi Hisano, Kazuki Sakamoto, Hiroki Takagi, Ryohei Terauchi, Kazuhiro Sato

    BMC Genomics18 ( 1 ) 125 - 125   2017年2月

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  • Quantitative Trait Loci and Maternal Effects Affecting the Strong Grain Dormancy of Wild Barley (Hordeum vulgare ssp. spontaneum)". 査読 国際誌

    Nakamura, S, Pourkheirandish, M, Morishige, H, Sameri, M, Sato, K, Komatsuda, T

    Frontiers in Plant Science8   1840 - 1840   2017年

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  • Genetic characterization of genetic resources of Aegilops tauschii, wheat D genome donor, newly collected in North Caucasia.

    Kakizaki, A, Kawahara,T, Zhuk, M.A, Smekalova, T.N, Sato, K, Abe, T, Sasanuma, T

    American Journal of Plant Science8   2769 - 2784   2017年

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  • Differences in dry matter production, grain production, and photosynthetic rate in barley cultivars under long-term salinity

    Tadashi Hirasawa, Kosuke Sato, Makoto Yamaguchi, Ryohei Narita, Asuka Kodama, Shunsuke Adachi, Taiichiro Ookawa, Kazuhiro Sato

    PLANT PRODUCTION SCIENCE20 ( 3 ) 288 - 299   2017年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Soil salinity is a major environmental stress causing significant loss of crop productivity. Barley (Hordeum vulgare L.) is one of the few field crops that can grow in salt-affected fields and varietal differences in productivity under salinity conditions were known. To clarify the trait most responsible for grain production under salt stress, barley cultivars that were salt tolerant (OUE812) or salt sensitive (OUC613) were grown from seedling to harvest stage in vermiculite containing various concentrations of NaCl. Dry weight of aboveground parts and grain weight decreased significantly with increasing NaCl concentration. The dry weight of the aboveground parts and grain weight decreased more significantly in OUC613 than in OUE812 for plants treated with 150 mM and 200 mM NaCl. A marked reduction in ripening percentage caused significantly decreased grain production in OUC613 as compared with OUE812. In plants treated with 200 mM NaCl, the photosynthetic rate decreased three weeks after starting the NaCl treatment, but a significant difference between cultivars in photosynthetic rate did not appear until seven weeks of NaCl treatment. OUE812 kept a higher photosynthetic rate during ripening than did OUC613 and dry matter production during the period from ripening to harvest was significantly larger in OUE812 than in OUC613. Keeping a higher photosynthetic rate might have contributed to higher grain production in OUE812. Higher ripening percentage and higher rate of photosynthesis during ripening might be target traits in breeding to improve the tolerance of barley to long-term salt stress.

    DOI: 10.1080/1343943X.2017.1343647

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  • Genomic regions responsible for amenability to Agrobacterium-mediated transformation in barley 査読 国際誌

    Hiroshi Hisano, Kazuhiro Sato

    Scientific Reports6   37505 - 37505   2016年11月

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    記述言語:英語  

    DOI: 10.1038/srep37505

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  • The HvNramp5 transporter mediates uptake of cadmium and manganese, but not iron 査読 国際誌

    Dezhi Wu, Naoki Yamaji, Miki Yamane, Miho Kashino-Fujii, Kazuhiro Sato, Jian Feng Ma

    Plant Physiology172 ( 3 ) 1899 - 1910   2016年11月

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    記述言語:英語  

    DOI: 10.1104/pp.16.01189

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  • Genetic analysis of the resistance of barley to cryptic species of Pyricularia

    Analiza Grubanzo Tagle, Izumi Chuma, Hiroshi Hisano, Kazuhiro Sato, Yukio Tosa

    JOURNAL OF GENERAL PLANT PATHOLOGY82 ( 6 ) 302 - 306   2016年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER JAPAN KK  

    The resistance of barley to Pyricularia oryzae isolates is controlled by the Rmo2 locus irrespective of their original hosts. The resistance of barley cultivar H.E.S.4 to isolate tO-7 of P. pennisetigena (a cryptic species in the P. oryzae/grisea species complex) cosegregated with the resistance to P. oryzae isolate GFSI-1-7-2 controlled by Rmo2.a. On the other hand, its resistance to isolate NI919, belonging to another cryptic species of Pyricularia, was controlled by another gene inherited independently from Rmo2.a. These results suggest that gene-for-gene interactions underlie the resistance of barley to cryptic species of Pyricularia.

    DOI: 10.1007/s10327-016-0687-2

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  • Mitochondrial genome sequences from wild and cultivated barley (Hordeum vulgare). 査読 国際誌

    Hiroshi Hisano, Mai Tsujimura, Hideya Yoshida, Toru Terachi, Kazuhiro Sato

    BMC genomics17 ( 1 ) 824 - 824   2016年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Sequencing analysis of mitochondrial genomes is important for understanding the evolution and genome structures of various plant species. Barley is a self-pollinated diploid plant with seven chromosomes comprising a large haploid genome of 5.1 Gbp. Wild barley (Hordeum vulgare ssp. spontaneum) and cultivated barley (H. vulgare ssp. vulgare) have cross compatibility and closely related genomes, although a significant number of nucleotide polymorphisms have been reported between their genomes. RESULTS: We determined the complete nucleotide sequences of the mitochondrial genomes of wild and cultivated barley. Two independent circular maps of the 525,599 bp barley mitochondrial genome were constructed by de novo assembly of high-throughput sequencing reads of barley lines H602 and Haruna Nijo, with only three SNPs detected between haplotypes. These mitochondrial genomes contained 33 protein-coding genes, three ribosomal RNAs, 16 transfer RNAs, 188 new ORFs, six major repeat sequences and several types of transposable elements. Of the barley mitochondrial genome-encoded proteins, NAD6, NAD9 and RPS4 had unique structures among grass species. CONCLUSIONS: The mitochondrial genome of barley was similar to those of other grass species in terms of gene content, but the configuration of the genes was highly differentiated from that of other grass species. Mitochondrial genome sequencing is essential for annotating the barley nuclear genome; our mitochondrial sequencing identified a significant number of fragmented mitochondrial sequences in the reported nuclear genome sequences. Little polymorphism was detected in the barley mitochondrial genome sequences, which should be explored further to elucidate the evolution of barley.

    DOI: 10.1186/s12864-016-3159-3

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  • Genome-wide identification of novel genetic markers from RNA sequencing assembly of diverse Aegilops tauschii accessions 査読 国際誌

    Ryo Nishijima, Kentaro Yoshida, Yuka Motoi, Kazuhiro Sato, Shigeo Takumi

    Molecular Genetics and Genomics291 ( 4 ) 1681 - 94   2016年8月

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  • Alanine aminotransferase controls seed dormancy in barley 査読 国際誌

    Kazuhiro Sato, Miki Yamane, Nami Yamaji, Hiroyuki Kanamori, Akemi Tagiri, Julian G. Schwerdt, Geoffrey B. Fincher, Takashi Matsumoto, Kazuyoshi Takeda, Takao Komatsuda

    Nature Communications7   11625 - 11625   2016年5月

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    記述言語:英語  

    DOI: 10.1038/ncomms11625

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  • Mitogen-Activated Protein Kinase Kinase 3 regulates grain dormancy in barley. 査読 国際誌

    Shingo Nakamura, Mohammad Pourkheirandish, Hiromi Morishige, Yuta Kubo, Masako Nakamura, Kazuya Ichimura, Shigemi Seo, Hiroyuki Kanamori, Jianzhong Wu, Tsuyu Ando, Goetz Hensel, Mohammad Sameri, Nils Stein, Kazuhiro Sato, Takashi Matsumoto, Masahiro Yano, Takao Komatsuda

    Current Biology26 ( 6 ) 775 - 81   2016年3月

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  • Improvement of barley genome annotations by deciphering the Haruna Nijo genome. 査読 国際誌

    Sato K, Tanaka T, Sigenobu S, Motoi Y, Wu J, Itoh T

    DNA Research23 ( 1 ) 21 - 8   2016年2月

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    記述言語:英語  

    DOI: 10.1093/dnares/dsv033

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  • Endogenous hormone levels affect the regeneration ability of callus derived from different organs in barley. 査読 国際誌

    Hiroshi Hisano, Takakazu Matsuura, Izumi C. Mori, Miki Yamane, Kazuhiro Sato

    Plant Physiol. Biochem.99   66 - 72   2016年2月

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  • The relationships between development and low temperature tolerance in barley near isogenic lines differing for flowering behavior. 査読

    Alfonso Cuesta-Marcos, María Muñoz-Amatriaín, Tanya Filichkin, Ildiko Karsai, Ben Trevaskis, Shozo Yasuda, Patrick Hayes, Kazuhiro Sato

    Plant and Cell Physiology56 ( 12 ) 2312 - 24   2015年12月

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    記述言語:英語  

    DOI: 10.1093/pcp/pcv147

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  • Genome-wide association mapping of cadmium accumulation in different organs of barley. 査読 国際誌

    Dezhi Wu, Kazuhiro Sato, Jian Feng Ma

    New Phytologist208 ( 3 ) 817 - 29   2015年11月

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    記述言語:英語  

    DOI: 10.1111/nph.13512

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  • A high-resolution physical map integrating an anchored chromosome with the BAC physical maps of wheat chromosome 6B 査読 国際誌

    Fuminori Kobayash他

    BMC Genomics16   595 - 595   2015年8月

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  • Evolution of the Grain Dispersal System in Barley 査読 国際誌

    Mohammad Pourkheirandish 他

    CELL162 ( 3 ) 527 - 39   2015年7月

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  • Purification of Bar-ley Dimeric α-Amylase Inhibitor-1 (BDAI-1) and Avenin-like Protein-a (ALP) from Beer and Their Impact on Beer Foam Stability. 査読 国際誌

    Takashi Iimure, Makoto Kihara, Kazuhiro Sato, Kensuke Ogushi

    Food Chemistry172   257 - 64   2015年4月

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  • Direct links between the vernalization response and other key traits of cereal crops. 査読 国際誌

    Deng, W, M. C. Casao, P. Wang, K. Sato, P. M. Hayes, E. J. Finnegan, B. Trevaskis

    Nature Communications6   5882 - 5882   2015年1月

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    記述言語:英語  

    DOI: 10.1038/ncomms6882

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  • Methylobacterium species promoting rice and barley growth and interaction specificity revealed with whole-cell MALDI-TOF/MS analysis. 査読 国際誌

    Akio Tani, Nurettin Sahin, Yoshiko Fujitani, Akiko Kato, Kazuhiro Sato, Kazuhide Kimbara

    PLOS ONE10 ( 6 ) e0129509   2015年

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  • Detection of QTLs for salt tolerance in Asian barley (Hordeum vulgare L.) by association analysis with SNP markers. 査読

    Hanen Sbei, Kazuhiro Sato, Tariq Shehzad, Moncef Harrabi, Kazutoshi Okuno

    Breed. Sci.64 ( 4 ) 378 - 88   2014年12月

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    記述言語:英語  

    DOI: 10.1270/jsbbs.64.378

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  • Genetic characterization of wild barley populations (Hordeum vulgare ssp. spontaneum) from Kazakhstan based on genome wide SNP analysis. 査読

    Yerlan Turuspekov, Saule Abugalieva, Kanat Ermekbayev, Kazuhiro Sato

    Breed. Sci.64 ( 4 ) 399 - 403   2014年12月

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    記述言語:英語  

    DOI: 10.1270/jsbbs.64.399

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  • Transcriptome analysis of barley identifies heat shock and HD-Zip I transcription factors up-regulated in response to multiple abiotic stresses

    Takashi Matsumoto, Hiromi Morishige, Tsuyoshi Tanaka, Hiroyuki Kanamori, Takao Komatsuda, Kazuhiro Sato, Takeshi Itoh, Jianzhong Wu, Shingo Nakamura

    MOLECULAR BREEDING34 ( 2 ) 761 - 768   2014年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Analyzing barley gene expression profiles in response to abiotic stress is critical to understanding how barley manages stress, and provides vital information to improve environmental stress tolerance for stable crop production. We developed an Agilent 60-mer oligo DNA microarray with 42,491 probe sets based on the sequences of 36,632 barley (Hordeum vulgare L.) full-length cDNA clones and conducted global expression profiling on barley seedlings subjected to desiccation, salt, cold and abscisic acid (ABA). We identified 281 genes that were differentially expressed in response to desiccation, salt, and cold stresses and ABA treatment. Among them, a class C heat shock transcription factor (HvHsfC1) and a homeodomain leucine zipper (HD-Zip) family I transcription factor (HvHox22) showed more than tenfold and fourfold higher expression, respectively, in response to the stimuli. Heat shock and HD-Zip transcription factors function as important regulators in stress responses in rice (Oryza sativa) and Arabidopsis thaliana; our results suggest these two transcription factors also play important roles in abiotic stress responses in barley. We mapped HvHox22 to the long arm of chromosome 2H and HvHsfC1 to the long arm of 4H, where drought resistance quantitative trait loci were previously detected. Our microarray data and identification of these stress response genes provide key information for dissection of the mechanism of abiotic stress tolerance in barley.

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  • Genome-wide marker development for the wheat D genome based on single nucleotide polymorphisms identified from transcripts in the wild wheat progenitor Aegilops tauschii

    Julio Cesar Masaru Iehisa, Akifumi Shimizu, Kazuhiro Sato, Ryo Nishijima, Kouhei Sakaguchi, Ryusuke Matsuda, Shuhei Nasuda, Shigeo Takumi

    THEORETICAL AND APPLIED GENETICS127 ( 2 ) 261 - 271   2014年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Key message 13,347 high-confidence SNPs were discovered through transcriptome sequencing of Aegilops tauschii, which are useful for genomic analysis and molecular breeding of hexaploid wheat.
    In organisms with large and complex genomes, such as wheat, RNA-seq analysis is cost-effective for discovery of genome-wide single nucleotide polymorphisms (SNPs). In this study, deep sequencing of the spike transcriptome from two Aegilops tauschii accessions representing two major lineages led to the discovery of 13,347 high-confidence (HC) SNPs in 4,872 contigs. After removing redundant SNPs detected in the leaf transcriptome from the same accessions in an earlier study, 10,589 new SNPs were discovered. In total, 5,642 out of 5,808 contigs with HC SNPs were assigned to the Ae. tauschii draft genome sequence. On average, 732 HC polymorphic contigs were mapped in silico to each Ae. tauschii chromosome. Based on the polymorphic data, we developed markers to target the short arm of chromosome 2D and validated the polymorphisms using 20 Ae. tauschii accessions. Of the 29 polymorphic markers, 28 were successfully mapped to 2DS in the diploid F-2 population of Ae. tauschii. Among ten hexaploid wheat lines, which included wheat synthetics and common wheat cultivars, 25 of the 43 markers were polymorphic. In the hexaploid F-2 population between a common wheat cultivar and a synthetic wheat line, 23 of the 25 polymorphic markers between the parents were available for genotyping of the F-2 plants and 22 markers mapped to chromosome 2DS. These results indicate that molecular markers that developed from polymorphisms between two distinct lineages of Ae. tauschii might be useful for analysis not only of the diploid, but also of the hexaploid wheat genome.

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  • Beer and wort proteomics 査読

    Takashi Iimure, Makoto Kihara, Kazuhiro Sato

    Methods in Molecular Biology1072   737 - 754   2014年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Humana Press Inc.  

    Proteome analysis provides a way to identify proteins related to the quality traits of beer. A number of protein species in beer and wort have been identified by two-dimensional gel electrophoresis combined with enzyme digestion such as trypsin, followed by mass spectrometry analyses and/or liquid chromatography mass/mass spectrometry. In addition, low molecular weight polypeptides in beer have been identified by the combination of non-enzyme digestion and mass analyses. These data sets of various molecular weight polypeptides (i.e., proteomes) provide a platform for analyzing protein functions in beer. Several novel proteins related to beer quality traits such as foam stability and haze formation have been identified by analyzing these proteomes. Some of the proteins have been applied to the development of efficient protein or DNA markers for trait selection in malting barley breeding. In this chapter, recent proteome studies of beer and wort are reviewed, and the methods and protocols of beer and wort proteome analysis are described. © 2014 Springer Science+Business Media, LLC.

    DOI: 10.1007/978-1-62703-631-3_51

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  • Genomic characterization of the Hordeum vulgare DEP1 (HvDEP1) gene and its diversity in a collection of barley accessions 査読

    Sébastien Bélanger, Mélanie Gauthier, Martine Jean, Kazuhiro Sato, François Belzile

    Euphytica198 ( 1 ) 29 - 41   2014年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Kluwer Academic Publishers  

    Grain yield potential in cereals results from the contributions of three main components: inflorescence number per plant, grain number per inflorescence and mean grain weight. In rice, the Dense and erect panicle (DEP1) gene affects three important quantitative traits of the inflorescence and DEP1 homologs have been reported in barley and wheat. In this work, we have performed a detailed characterization of the barley DEP1 (HvDEP1) gene. We obtained a full-length sequence of its transcript, examined its expression in various tissues, genetically mapped it to chromosome 5H and measured its nucleotide diversity in a collection of barley cultivars from Canada. This gene has an exceptionally long 5′ UTR (297 bp) that contains an upstream open reading frame (uORF) coding for a predicted peptide of 70 amino acids. A similarly long 5′ UTR containing a putative uORF was found in all six grass species that we examined. Although HvDEP1 transcripts could be detected in all tissues tested, it was highest in meristematic tissues. In a collection of 167 barley cultivars, a low level of polymorphism was observed (π = 0.001) and the 37 SNPs/indels detected, none of which result in changes in the amino acid sequence of the HvDEP1 protein, defined 8 haplotypes. Diversity was especially low among 83 six-row types, all but one of which carried the exact same haplotype. We suggest that HvDEP1 is involved in the control of cellular growth and differentiation, that the conserved uORF may play a role in its post-transcriptional regulation and that this gene has undergone a very different history of selection within the two-row and six-row Canadian elite germplasm. © 2014 Springer Science+Business Media Dordrecht.

    DOI: 10.1007/s10681-014-1089-1

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  • Mapping nonrecombining regions in barley using multicolor FISH

    M. Karafiatova, J. Bartos, D. Kopecky, L. Ma, K. Sato, A. Houben, N. Stein, J. Dolezel

    CHROMOSOME RESEARCH21 ( 8 ) 739 - 751   2013年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Fluorescence in situ hybridization (FISH) is a widely used method to localize DNA sequences on chromosomes. Out of the many uses, FISH facilitates construction of physical maps by ordering contigs of large-insert DNA clones, typically bacterial artificial chromosome (BAC) and establishing their orientation. This is important in genomic regions with low recombination frequency where genetic maps suffer from poor resolution. While BAC clones can be mapped directly by FISH in plants with small genomes, excess of repetitive DNA hampers this application in species with large genomes. Mapping single-copy sequences such as complementary DNA (cDNA) is an attractive alternative. Unfortunately, localization of single-copy sequences shorter than 10 kb remains a challenging task in plants. Here, we present a highly efficient FISH technique that enables unambiguous localization of single copy genes. We demonstrated its utility by mapping 13 out of 15 full-length cDNAs of variable length (2,127-3,400 bp), which were genetically defined to centromeric and pericentromeric regions of barley chromosome 7H. We showed that a region of 1.2 cM (0.7 %) on genetic map represented more than 40 % of the physical length of the chromosome. Surprisingly, all cDNA probes occasionally revealed hybridization signals on other chromosomes, indicating the presence of partially homologous sequences. We confirmed the order of 10 cDNA clones and suggested a different position for three cDNAs as compared to published genetic order. These results underline the need for alternative approaches such as FISH, which can resolve the order of markers in genomic regions where genetic mapping fails.

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  • Beer proteomics analysis for beer quality control and malting barley breeding

    Takashi Iimure, Kazuhiro Sato

    FOOD RESEARCH INTERNATIONAL54 ( 1 ) 1013 - 1020   2013年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    A series of proteomic procedures has been applied to analyze beer and wort proteomes including two-dimensional gel electrophoresis combined with mass spectrometry (MS), pre-fractionation followed by gel electrophoresis and MS, and gel-free based shotgun liquid chromatography-MS/MS. These approaches have detected a number of protein species in beer and wort, from barley and yeast, and occasionally from rice and maize. Of these beer proteins, barley dimeric alpha-amylase inhibitor-1 (BDAI-1), barley trypsin inhibitor-CMe precursor (BTI-CMe) and yeast thioredoxin have been identified as factors responsible for beer quality traits of foam and haze. In addition, a number of hordein derived polypeptides involved to celiac disease have also been identified. Among these proteins and peptides, the modifications such as glycation and partial digestion in several proteins such as lipid transfer protein (LW) and protein Z have been characterized. These findings may provide novel tools to improve quality control during beer processing and they may enhance selection in malting barley breeding. (C) 2012 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.foodres.2012.11.028

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  • Barley genome and beer proteome

        2013年

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  • Discovery of High-Confidence Single Nucleotide Polymorphisms from Large-Scale De Novo Analysis of Leaf Transcripts of Aegilops tauschii, A Wild Wheat Progenitor

    Iehisa Julio Cesar Masaru, Shimizu Akifumi, Sato Kazuhiro, Nasuda Shuhei, Takumi Shigeo

    DNA RESEARCH19 ( 6 ) 487 - 497   2012年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Construction of high-resolution genetic maps is important for genetic and genomic research, as well as for molecular breeding. Single nucleotide polymorphisms (SNPs) are the,predominant class of genetic variation and can be used as molecular markers. Aegilops tauschii, the D-genome donor of common wheat, is considered a valuable genetic resource for wheat improvement. Our previous study implied that Ae. tauschii accessions can be genealogically divided into two major lineages. In this study, the transcriptome of two Ae. tauschii accessions from each lineage, lineage 1 (L1) and 2 (L2), was sequenced, yielding 9435 SNPs and 739 insertion/deletion polymorphisms (indels) after de novo assembly of the reads. Based on 36 contig sequences, 31 SNPs and six indels were validated on 20 diverse Ae. tauschii accessions. Because almost all of the SNP markers were polymorphic between L1 and L2, and the D-genome donor of common wheat is presumed to belong to L2, these markers are available for D-genome typing in crosses between common wheat varieties and L1-derived synthetic wheat. Due to the conserved synteny between wheat and barley chromosomes, the high-density expressed sequence tag barley map and the hypothetical gene order in barley can be applied to develop markers on target chromosomal regions in wheat.

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  • A physical, genetic and functional sequence assembly of the barley genome

    Klaus F. X. Mayer, Robbie Waugh, Peter Langridge, Timothy J. Close, Roger P. Wise, Andreas Graner, Takashi Matsumoto, Kazuhiro Sato, Alan Schulman, Gary J. Muehlbauer, Nils Stein, Ruvini Ariyadasa, Daniela Schulte, Naser Poursarebani, Ruonan Zhou, Burkhard Steuernagel, Martin Mascher, Uwe Scholz, Bujun Shi, Peter Langridge, Kavitha Madishetty, Jan T. Svensson, Prasanna Bhat, Matthew Moscou, Josh Resnik, Timothy J. Close, Gary J. Muehlbauer, Pete Hedley, Hui Liu, Jenny Morris, Robbie Waugh, Zeev Frenkel, Avraham Korol, Helene Berges, Andreas Graner, Nils Stein, Burkhard Steuernagel, Stefan Taudien, Marco Groth, Marius Felder, Stefano Lonardi, Denisa Duma, Matthew Alpert, Francesa Cordero, Marco Beccuti, Gianfranco Ciardo, Yaqin Ma, Steve Wanamaker, Nils Stein, Timothy J. Close, Matthias Platzer, John W. S. Brown, Alan Schulman, Matthias Platzer, Geoffrey B. Fincher, Gary J. Muehlbauer, Kazuhiro Sato, Stefan Taudien, Dharanya Sampath, David Swarbreck, Simone Scalabrin, Andrea Zuccolo, Vera Vendramin, Michele Morgante, Alan Schulman

    NATURE491 ( 7426 ) 711 - +   2012年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Barley (Hordeum vulgare L.) is among the world's earliest domesticated and most important crop plants. It is diploid with a large haploid genome of 5.1 gigabases (Gb). Here we present an integrated and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a structured whole-genome context. We developed a physical map of 4.98 Gb, with more than 3.90 Gb anchored to a high-resolution genetic map. Projecting a deep whole-genome shotgun assembly, complementary DNA and deep RNA sequence data onto this framework supports 79,379 transcript clusters, including 26,159 'high-confidence' genes with homology support from other plant genomes. Abundant alternative splicing, premature termination codons and novel transcriptionally active regions suggest that post-transcriptional processing forms an important regulatory layer. Survey sequences from diverse accessions reveal a landscape of extensive single-nucleotide variation. Our data provide a platform for both genome-assisted research and enabling contemporary crop improvement.

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  • Genetic diversity analysis of hulless barley from Shangri-la region revealed by SSR and AFLP markers

    Yu Guo, Yali Li, Yuan Huang, Devra Jarvis, Kazuhiro Sato, Kenji Kato, Hiroshi Tsuyuzaki, Lijuan Chen, Chunlin Long

    GENETIC RESOURCES AND CROP EVOLUTION59 ( 7 ) 1543 - 1552   2012年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    For adding the hulless barley resources of Shangri-la region to the global barley resource library, a basic work was done by us to assess their genetic diversity of this region. The genetic diversity of 60 hulless barley samples collected from three counties in Shangri-la region of Yunnan Province, were studied using SSR (simple sequence repeats) and AFLP (amplified fragment length polymorphism) markers. A total of 70 alleles were detected for 19 pairs of SSR primers, and 525 band containing 464 polymorphic bands were revealed for 5 pairs of AFLP primers. The value of polymorphism information content (PIC) ranged from 0.03 to 0.86 for SSR primers. The total numbers of alleles were 51, 55, 43 in three populations and the polymorphic bands were 188, 205 and 141. The genetic distances and genetic identity among the three populations showed their close relationship. The gene diversity among populations relative to the total population diversity (Gst) was 0.13 for SSR markers and 0.02 for AFLP markers and indicated that just 13 and 2% variations were among populations, respectively. The UPGMA cluster analysis revealed that all of the samples grouped randomly rather than clustered into distinct groups corresponding to their populations, row types and spring/fall types. We concluded that there was high genetic diversity in the population of Shangri-la region and the formation of diversity was related to complex environment and inhabitants' traditional practices.

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  • A SHORT INTERNODES (SHI) family transcription factor gene regulates awn elongation 1 and pistil morphology in barley 査読 国際誌

    Takahisa Yuo, Yuko Yamashita, Hiroyuki Kanamori, Takashi Matsumoto, Udda Lundqvist, Kazuhiro Sato, Masahiko Ichii, Stephen A. Jobling, Shin Taketa

    Journal of Experimental Botany63 ( 14 ) 5223 - 32   2012年9月

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    記述言語:英語  

    DOI: 10.1093/jxb/ers182

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  • Malting quality quantitative trait loci on a high-density map of Mikamo golden x Harrington cross in barley (Hordeum vulgare L.) 査読

    Tian-su Zhou, Iimure Takashi, Kanatani Ryouichi, Hirota Naohiko, Kihara Makoto, Hoki Takehiro, Sato Kazuhiro

    MOLECULAR BREEDING30 ( 1 ) 103 - 112   2012年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    A high-density map consisting of 550 markers was constructed based on the segregation data of 95 doubled-haploid lines (DHLs) derived from the cross between a Japanese barley cultivar, Mikamo Golden and a North American barley cultivar, Harrington (MH-DHLs). Quality traits of malt extract (EX), total nitrogen (TN), soluble nitrogen (SN), Kolbach index (KI), diastatic power (DP), wort beta-glucan (WG) and viscosity (VS) were determined in three site/year crops. Quantitative trait loci (QTL) analyses were performed with these quality data sets, using the linkage map. Major QTL controlling EX, SN and KI were mapped on terminal region of 5H with Harrington as effective allele. Another QTL controlling EX was mapped on 2H with Mikamo Golden as effective allele. QTL controlling TN, DP, WG and VS were detected variably in terms of flanking markers and chromosomes depending on site/year. Cleaved amplified polymorphic sequences (CAPS) markers for EX based on the QTL detected on 2H and 5H were developed. Analysis of EX and genotypes of 33 malting barley cultivars from around the world as well as MH-DHLs revealed that the two CAPS marker on 2H and 5H affect EX by a significant difference, suggesting that the two CAPS markers were valuable for marker-assisted selection in malting barley breeding.

    DOI: 10.1007/s11032-011-9602-x

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  • Acquisition of aluminum tolerance by modification of a single gene in barley 査読 国際誌

    Miho Fujii, Kengo Yokosho, Naoki Yamaji, Daisuke Saisho, Miki Yamane, Hirokazu Takaha-shi, Kazuhiro Sato, Mikio Nakazono, Jian Feng Ma

    Nature Communications3   713 - 713   2012年3月

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    記述言語:英語  

    DOI: 10.1038/ncomms1726

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  • Mutation analysis of barley malt protein Z4 and protein Z7 on beer foam stability. 査読 国際誌

    Takashi Iimure, Tatsuji Kimura, Shigeki Araki, Makoto Kihara, Masahide Sato, Shinji Yamada, Tatsuro Shigyou, Kazuhiro Sato

    J. Agr. Food Chem.60 ( 6 ) 1548 - 54   2012年2月

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    記述言語:英語  

    DOI: 10.1021/jf2044718

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  • Proteome analysis of the wort boiling process

    Takashi Iimure, Nami Nankaku, Makoto Kihara, Shinji Yamada, Kazuhiro Sato

    FOOD RESEARCH INTERNATIONAL45 ( 1 ) 262 - 271   2012年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    A comprehensive proteome map was constructed of brewers wort. The map consisted of protein identification on two-dimensional gel electrophoresis (2DE) images and identified 63 out of 202 protein spots, which were categorized into 20 protein species. To analyze the modification of protein Z during wort boiling, protein Z spots on 2DE gel of the sweet wort, the boiled wort and the trub were analyzed by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI TOF-MS) and then the spectra were compared. The analysis identified several specific signals detected only in the trub, suggesting that specific modification occurred in the precipitated protein Z during wort boiling. The protein Z spot on the 2DE gel of the precipitate was further analyzed by liquid chromatography mass spectrometry/mass spectrometry. The analysis identified low molecular weight fragment (1.3 kDa) derived from wound induced protein (barwin) in the protein Z spot of the trub. These results suggested that protein Z was precipitated by binding with comparatively small size specific fragment(s) derived from sweet wort protein, i.e., barwin during wort boiling. Our results and understandings have application for quality assurance and control in commercial brewing practice, and development of DNA markers for malting barley breeding. (C) 2011 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.foodres.2011.10.033

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  • A wheat homologue of PHYTOCLOCK 1 is a candidate gene conferring the early heading phenotype to einkorn wheat. 査読

    Nobuyuki Mizuno, Miyuki Nitta, Kazuhiro Sato, Shuhei Nasuda

    Genes and Genetic Systems87 ( 6 ) 357 - 67   2012年

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    記述言語:英語  

    DOI: 10.1266/ggs.87.357

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  • An Improved Consensus Linkage Map of Barley Based on Flow-Sorted Chromosomes and Single Nucleotide Polymorphism Markers

    Maria Munoz-Amatriain, Matthew J. Moscou, Prasanna R. Bhat, Jan T. Svensson, Jan Bartos, Pavla Suchankova, Hana Simkova, Takashi R. Endo, Raymond D. Fenton, Stefano Lonardi, Ana M. Castillo, Shiaoman Chao, Luis Cistue, Alfonso Cuesta-Marcos, Kerrie L. Forrest, Matthew J. Hayden, Patrick M. Hayes, Richard D. Horsley, Kihara Makoto, David Moody, Kazuhiro Sato, Maria P. Valles, Brande B. H. Wulff, Gary J. Muehlbauer, Jaroslav Dolezel, Timothy J. Close

    PLANT GENOME4 ( 3 ) 238 - 249   2011年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CROP SCIENCE SOC AMER  

    Recent advances in high-throughput genotyping have made it easier to combine information from different mapping populations into consensus genetic maps, which provide increased marker density and genome coverage compared to individual maps. Previously, a single nucleotide polymorphism (SNP)-based genotyping platform was developed and used to genotype 373 individuals in four barley (Hordeum vulgare L.) mapping populations. This led to a 2943 SNP consensus genetic map with 975 unique positions. In this work, we add data from six additional populations and more individuals from one of the original populations to develop an improved consensus map from 1133 individuals. A stringent and systematic analysis of each of the 10 populations was performed to achieve uniformity. This involved reexamination of the four populations included in the previous map. As a consequence, we present a robust consensus genetic map that contains 2994 SNP loci mapped to 1163 unique positions. The map spans 1137.3 cM with an average density of one marker bin per 0.99 cM. A novel application of the genotyping platform for gene detection allowed the assignment of 2930 genes to flow-sorted chromosomes or arms, confirmed the position of 2545 SNP-mapped loci, added chromosome or arm allocations to an additional 370 SNP loci, and delineated pericentromeric regions for chromosomes 2H to 7H. Marker order has been improved and map resolution has been increased by almost 20%. These increased precision outcomes enable more optimized SNP selection for marker-assisted breeding and support association genetic analysis and map-based cloning. It will also improve the anchoring of DNA sequence scaffolds and the barley physical map to the genetic map.

    DOI: 10.3835/plantgenome2011.08.0023

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  • Indigenous knowledge and traditional conservation of hulless barley (Hordeum vulgare) germplasm resources in the Tibetan communities of Shangri-la, Yunnan, SW China

    Yali Li, Chunlin Long, Kenji Kato, Chunyan Yang, Kazuhiro Sato

    GENETIC RESOURCES AND CROP EVOLUTION58 ( 5 ) 645 - 655   2011年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Hulless barley (Hordeum vulgare L. s. l.) is the staple food crop of Tibetan communities in the Qinghai-Tibet Plateau, covering Tibet, Qinghai, Gansu, Sichuan and Yunnan provinces in China. Participatory Rural Appraisal methodolgies were employed in twenty-seven villages that were randomly selected in Shangri-la region, or Diqing Prefecture of Yunnan Province to document the ethnobotanical and indigenous knowledge related to the production, diversity, use and conservation of hulless barley. For Tibetans in Shangri-la, the crop has a high socio-cultural value. The genetic diversity of landraces managed by the farmers in Shangri-la is an extremely important germplasm resource. We recorded a total of 54 landraces that are primarily described by farmers based on their cooking quality, growth cycle, color and size of the grains. The ethnobotanical and indigenous knowledge recorded in this study contributes to the understanding of the genetic diversity of hulless barley in Shangri-la and even in the Qinghai-Tibet Plateau, and in defining appropriate strategies for its conservation on farm.

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  • 454 sequencing of pooled BAC clones on chromosome 3H of barley. 査読 国際誌

    Kazuhiro Sato, Yuka Motoi, Nami Yamaji, Hideya Yoshida

    BMC Genomics12   246 - 246   2011年5月

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  • Construction and sequence analysis of barley 24,783 full-length cDNAs. 査読 国際誌

    Matsumoto,T, T.Tanaka, H.Sakai, N.Amano, H.Kanamori, K.Kurita, A.Kikuta, K.Kamiya, M.Yamamoto, H.Ikawa, N.Fujii, K. Hori, T.Itoh, K. Takeda, K.Sato

    Plant Physiology156 ( 1 ) 20 - 8   2011年5月

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    記述言語:英語  

    DOI: 10.1104/pp.110.171579

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  • Single nucleotide polymorphism mapping and alignment of recombinant chromosome substitution lines in barley. 査読

    Kazuhiro Sato, Timothy J Close, Prasanna Bhat, María Muñoz-Amatriaín, Gary J Muehlbauer

    Plant & cell physiology52 ( 5 ) 728 - 37   2011年5月

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    記述言語:英語  

    Single nucleotide polymorphism (SNP) genotyping is useful for assessing genetic variation in germplasm collections, genetic map development and detection of alien chromosome substitutions. In this study, a diversity analysis using 1,301 SNPs on a set of 37 barley accessions was conducted. This analysis showed a high polymorphism rate between the malting barley cultivar 'Haruna Nijo' and the food barley cultivar 'Akashinriki'. Haruna Nijo and Akashinriki are donors of the barley expressed sequence tag (EST) collections. A doubled haploid (DH) population derived from the cross between Haruna Nijo and Akashinriki was genotyped with 1,448 SNPs. Of these 1,448 SNPs, 734 were polymorphic and distributed on barley linkage groups (chromosomes) as follows: 1H (86), 2H (125), 3H (120), 4H (100), 5H (127), 6H (88) and 7H (88). By using cMAP, we integrated the SNP markers across high-density maps. The SNPs were also used to genotype 98 BC(3)F(4) recombinant chromosome substitution lines (RCSLs) developed from the same cross (Haruna Nijo/Akashinriki). These data were used to create graphical genotypes for each line and thus estimate the location, extent and total number of introgressions from Akashinriki in the Haruna Nijo background. The 35 selected RCSLs sample most of the Akashinriki food barley genome, with only a few missing segments. These resources bring new alleles into the malting barley gene pool from food barley.

    DOI: 10.1093/pcp/pcr024

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  • Isolation and Characterization of a Barley Yellow Stripe-like Gene, HvYSL5. 査読

    Zheng, Luqing, Fujii, Miho, Yamaji, Naoki, Sasaki, Akimasa, Yamane, Miki, Sakurai, Isamu, Sato, Kazuhiro, MA, Jian Feng

    Plant and Cell Physiology52 ( 5 ) 765 - 74   2011年5月

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    記述言語:英語  

    DOI: 10.1093/pcp/pcr009

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  • Natural variation of barley vernalization requirements: Implication of quantitative variation of winter growth habit as an adaptive trait in East Asia. 査読

    Daisuke Saisho, Makoto Ishii, Kiyosumi Hori, Kazuhiro Sato

    Plant and Cell Physiology52 ( 5 ) 775 - 84   2011年5月

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    記述言語:英語  

    DOI: 10.1093/pcp/pcr046

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  • Genome Analysis: The State of Knowledge of Barley Genes 査読

    Arnis Druka, Kazuhiro Sato, Gary J. Muehlbauer

    Barley: Production, Improvement, and Uses   85 - 111   2011年4月

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    記述言語:英語   掲載種別:論文集(書籍)内論文   出版者・発行元:Wiley-Blackwell  

    DOI: 10.1002/9780470958636.ch4

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  • Unlocking the Barley Genome by Chromosomal and Comparative Genomics

    Klaus F. X. Mayer, Mihaela Martis, Pete E. Hedley, Hana Simkova, Hui Liu, Jenny A. Morris, Burkhard Steuernagel, Stefan Taudien, Stephan Roessner, Heidrun Gundlach, Marie Kubalakova, Pavla Suchankova, Florent Murat, Marius Felder, Thomas Nussbaumer, Andreas Graner, Jerome Salse, Takashi Endo, Hiroaki Sakai, Tsuyoshi Tanaka, Takeshi Itoh, Kazuhiro Sato, Matthias Platzer, Takashi Matsumoto, Uwe Scholz, Jaroslav Dolezel, Robbie Waugh, Nils Stein

    PLANT CELL23 ( 4 ) 1249 - 1263   2011年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    We used a novel approach that incorporated chromosome sorting, next-generation sequencing, array hybridization, and systematic exploitation of conserved synteny with model grasses to assign; similar to 86% of the estimated; similar to 32,000 barley (Hordeum vulgare) genes to individual chromosome arms. Using a series of bioinformatically constructed genome zippers that integrate gene indices of rice (Oryza sativa), sorghum (Sorghum bicolor), and Brachypodium distachyon in a conserved synteny model, we were able to assemble 21,766 barley genes in a putative linear order. We show that the barley (H) genome displays a mosaic of structural similarity to hexaploid bread wheat (Triticum aestivum) A, B, and D subgenomes and that orthologous genes in different grasses exhibit signatures of positive selection in different lineages. We present an ordered, information-rich scaffold of the barley genome that provides a valuable and robust framework for the development of novel strategies in cereal breeding.

    DOI: 10.1105/tpc.110.082537

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  • Expression and functional analysis of the barley Nud gene using transgenic rice

    Katsuyuki Kakeda, Norimitsu Ishihara, Yohei Izumi, Kazuhiro Sato, Shin Taketa

    BREEDING SCIENCE61 ( 1 ) 35 - 42   2011年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPANESE SOC BREEDING  

    Most cereal crops have hulless grains (naked caryopses) with a free-threshing trait, whereas the majority of barley cultivars show hulled (covered) caryopses. The naked caryopsis in barley is genetically controlled by a single locus, nod. The Nud gene (the covered caryopsis allele) encodes an ethylene response factor (ERF) family transcription factor that regulates a lipid biosynthetic pathway. For functional analysis of the barley Nud gene, we produced transgenic rice expressing Nod in the developing caryopses. All transgenic lines had caryopses that were easily dehulled at maturity, indicating that the naked caryopsis phenotype remained in spite of expression of the Nod transgene. Histochemical and lipid analyses of the transgenic rice caryopses did not show increased lipid accumulation on the surface of developing caryopses, suggesting that the Nud-mediated lipid pathway may not function in rice caryopses. The predicted rice ortholog of Nod, Os06ERF was expressed specifically in the developing caryopses. However, expression of Os06ERF ceased at an earlier developmental stage than that of the native Nod gene in barley caryopses, which was also the case for expression of the Nod transgene. This raises the alternative hypothesis that the timing of Nod expression may be critical for activating the pathway for hull-caryopsis adhesion.

    DOI: 10.1270/jsbbs.61.35

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  • Development of DNA Markers Associated with Beer Foam Stability for Barley Breeding. 査読 国際誌

    Iimure Takashi, Makoto Kihara, Seiichiro Ichikawa, Kazutoshi Ito, Kazuyoshi Takeda, Kazuhiro Sato

    Theor. Appl. Genet.122 ( 1 ) 199 - 210   2011年1月

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  • Malting quality quantitative trait loci on a high density map of Mikamo Golden x Harrington cross in barley (Hordeum vulgare L.).

    Tian-su Zhou, Iimure Takashi, Hirota Naohiko, Kihara Makoto, Hoki Takehiro, Kanatani Ry-ouichi, Sato Kazuhiro

    Molecular Breeding61   35 - 42   2011年

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  • Development of genetic map and alignment of recombinant chromosome substitu-tion lines from a cross of EST donors by high accuracy SNP typing in barley.

    Kazuhiro Sato, Timothy J. Close, Prasanna Bhat, María Muñoz-Amatriaín, Gary J. Muehlbauer

    Plant and Cell Physiology52   728 - 737   2011年

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  • NBRP, National Bioresource Project of Japan and plant bioresource management

    Nori Kurata, Hikaru Satoh, Hidemi Kitano, Yasuo Nagato, Takashi Endo, Kazuhiro Sato, Ryo Akashi, Hiroshi Ezura, Makoto Kusaba, Masatomo Kobayashi, Eiji Nitasaka, Fumie Kasai, Yukiko Yamazaki, Atsushi Yoshimura

    BREEDING SCIENCE60 ( 5 ) 461 - 468   2010年12月

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    記述言語:英語   出版者・発行元:JAPANESE SOC BREEDING  

    The National BioResource Project has been organized and established to promote research activities using valuable bioresources. A total of twenty-eight bioresources for ten animals, nine plants and nine microorganisms/cell lines developed or collected in Japan were selected for the project. Resources are categorized into several different groups in the project; genetic resources, germplasm, genome resources and their information. Choices of how many resources must be preserved and maintained and in which categories are dependent on the status of the research community of each organism. These resources, if utilized systematically and intelligently, are powerful means for leading new scientific discoveries. Some examples can be seen in this paper. This paper reviews plant bioresources with the main focus on rice resource activities within the project.

    DOI: 10.1270/jsbbs.60.461

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  • Construction of a novel beer proteome map and its use in beer quality control

    Takashi Iimure, Nami Nankaku, Naohiko Hirota, Zhou Tiansu, Takehiro Hoki, Makoto Kihara, Katsuhiro Hayashi, Kazutoshi Ito, Kazuhiro Sato

    FOOD CHEMISTRY118 ( 3 ) 566 - 574   2010年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI LTD  

    Beer proteins were analysed by two-dimensional gel electrophoresis (2DE). The protein species associated with major spots on 2DE gels were identified by mass spectrometry followed by a database search to construct a comprehensive beer proteome map. As a result, 85 out of 199 protein spots examined were positively identified and categorised into 12 protein species. A total of I I beer samples were brewed from the malt of eight cultivars having different levels of protein modification. This experiment was designed to demonstrate the influences of barley cultivar and malt modification on beer protein composition and beer quality characters. The beers produced from these brewing trails were subsequently analysed by 2DE and their proteomes were compared. Cultivars and malt modification affected the concentration of several proteins in beer. Beer protein concentration was associated with differences in the desirable beer quality trait, foam stability, In addition, expression of yeast derived proteins were observed that may also influence beer quality. Overall, the application of a comprehensive beer proteome map provides a strong platform for detection and potential manipulation of beer quality related proteins. (C) 2009 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.foodchem.2009.05.022

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  • NBRP databases: databases of biological resources in Japan

    Yukiko Yamazaki, Ryo Akashi, Yutaka Banno, Takashi Endo, Hiroshi Ezura, Kaoru Fukami-Kobayashi, Kazuo Inaba, Tadashi Isa, Katsuhiko Kamei, Fumie Kasai, Masatomo Kobayashi, Nori Kurata, Makoto Kusaba, Tetsuro Matuzawa, Shohei Mitani, Taro Nakamura, Yukio Nakamura, Norio Nakatsuji, Kiyoshi Naruse, Hironori Niki, Eiji Nitasaka, Yuichi Obata, Hitoshi Okamoto, Moriya Okuma, Kazuhiro Sato, Tadao Serikawa, Toshihiko Shiroishi, Hideaki Sugawara, Hideko Urushibara, Masatoshi Yamamoto, Yoshio Yaoita, Atsushi Yoshiki, Yuji Kohara

    NUCLEIC ACIDS RESEARCH38   D26 - D32   2010年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    The National BioResource Project (NBRP) is a Japanese project that aims to establish a system for collecting, preserving and providing bioresources for use as experimental materials for life science research. It is promoted by 27 core resource facilities, each concerned with a particular group of organisms, and by one information center. The NBRP database is a product of this project. Thirty databases and an integrated database-retrieval system (BioResource World: BRW) have been created and made available through the NBRP home page (http://www.nbrp.jp). The 30 independent databases have individual features which directly reflect the data maintained by each resource facility. The BRW is designed for users who need to search across several resources without moving from one database to another. BRW provides access to a collection of 4.5-million records on bioresources including wild species, inbred lines, mutants, genetically engineered lines, DNA clones and so on. BRW supports summary browsing, keyword searching, and searching by DNA sequences or gene ontology. The results of searches provide links to online requests for distribution of research materials. A circulation system allows users to submit details of papers published on research conducted using NBRP resources.

    DOI: 10.1093/nar/gkp996

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  • Cereal Resources in National BioResource Project of Japan

    Kazuhiro Sato, Takashi R. Endo, Nori Kurata

    Interdisciplinary Bio Central   2010年

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  • Novel barley (Hordeum vulgare L.) germplasm resistant to acidic soil

    J. S. Moroni, K. Sato, B. J. Scott, M. Conyers, B. J. Read, R. Fisher, G. Poile

    CROP & PASTURE SCIENCE61 ( 7 ) 540 - 553   2010年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CSIRO PUBLISHING  

    Improving the resistance of barley (Hordeum vulgare L.) to acidic soils is an important goal of several barley breeding programs around the world. The identification and utilisation of novel barley sources resistant to aluminium (Al) may provide a significant and rapid advance towards that goal. Barley standards and screening protocols for selecting barley germplasm resistant to Al in nutrient solution and acidic soil were reevaluated. The assays used were quantitative in nature and were suitable for genotypic- and seedling-based selections. Although there was a broad agreement between the solution culture assays and soil assays in the ranking of genotypes it obscured the fact that misclassification of genotypes is common. Brindabella was shown to be better suited than Dayton (the current barley standard resistant to Al) as the Australian standard for resistance to acidic soils. A seedling-based Al pulse-recovery assay and an acidic soil assay were used to characterise 41 genotypes from the South and East Asian Barley Core Collection (SEA-BCC). In addition, in the acidic soil assays several standard barley and wheat genotypes were included. Three SEA-BCC genotypes were more resistant than Dayton to acidic soil while several others were similar to Dayton. The most resistant SEA-BCC genotypes Honen, Ohichi and Zairai Tanbo were of Japanese origin. Misclassification of barley genotypes and wheat genotypes for resistance to soil acidity between solution culture and acid soil assay provided strong evidence for the unsuitability of solution culture assay. Although in solution culture several barley genotypes were sensitive relative to wheat, in acidic soil they were not different from wheat. While the quest for resistant barley to acidic soils similar or better than resistant wheat still continues, it may be an unnecessary endeavour.

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  • Development and implementation of high-throughput SNP genotyping in barley. 査読 国際誌

    Timothy J Close, Prasanna R Bhat, Stefano Lonardi, Yonghui Wu, Nils Rostoks, Luke Ramsay, Arnis Druka, Nils Stein, Jan T Svensson, Steve Wanamaker, Serdar Bozdag, Mikeal L Roose, Matthew J Moscou, Shiaoman Chao, Rajeev Varshney, Peter Szucs, Kazuhiro Sato, Patrick M Hayes, David E Matthews, Andris Kleinhofs, Gary J Muehlbauer, Joseph DeYoung, David F Marshall, Kavitha Madishetty, Raymond, D Fenton, Pascal Condamine, Andreas Graner, Robbie Waugh

    BMC Genomics10   582 - 582   2009年12月

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  • Mapping of QTL for intermedium spike on barley chromosome 4H using EST-based markers

    Fahimeh Shahinnia, Badraldin Ebrahim Sayed-Tabatabaei, Kazuhiro Sato, Mohammad Pourkheirandish, Takao Komatsuda

    BREEDING SCIENCE59 ( 4 ) 383 - 390   2009年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPANESE SOC BREEDING  

    The lateral spikelets of two-rowed barley are reduced in size and sterile, but in six-rowed barley all three spikelets are fully fertile. The trait is largely controlled by alleles at the vrs1 locus on chromosome arm 2HL, as modified by the allele present at the I locus on chromosome arm 4HS. Molecular markers were developed to saturate the 4HS region by exploiting expressed sequence-tags, either previously mapped in barley to this region, or present in the syntenic region of rice chromosome 3. Collinearity between rice and barley was strong in the 4.8 cM interval BJ468164-AV933435 and the 10 cM interval AV942364-BJ455560. A major QTL for lateral spikelet fertility (the I locus) explained 44% of phenotypic variance, and was located in the interval CB873567-BJ473916. The genotyping of near-isogenic lines for I placed the locus in a region between CB873567 and EBmac635, and therefore the most likely position of the I locus was proximal to CB873567 in a 5.3 cM interval between CB873567-BJ473916.

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  • Validation of rice blast resistance genes in barley using a QTL mapping population and near-isolines

    Phinyarat Kongprakhon, Alfonso Cuesta-Marcos, Patrick M. Hayes, Kelley L. Richardson, Pattama Sirithunya, Kazuhiro Sato, Brian Steffenson, Theerayuth Toojinda

    BREEDING SCIENCE59 ( 4 ) 341 - 349   2009年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPANESE SOC BREEDING  

    There are prior reports of Pyricularia grisea-the causal agent of blast of rice-causing disease in barley. In order to determine the specificity of this resistance in barley, we extended our previous mapping efforts to include blast isolates from barley and rice grown in Thailand and we assessed two resistance phenotypes: leaf blast (LB) and neck blast (NB). The largest-effect resistance QTL, on chromosome I H, was associated with NB and LB and is located in a region rich in resistance genes, including QTL conferring resistance to stripe rust (incited by Puccinia striiformis f. sp. hordei) and the mildew (Blumeria graminis f. sp. hordei) resistance gene Mla. The LB, NB and mildew resistance alleles trace to one parent (Baronesse) whereas the stripe rust resistance allele traces to the other parent (BCD47) of the mapping population. Baronesse is the susceptible recurrent parent of a set of near-isogenic lines (NILs) for three stripe rust resistance QTL, including one on 1H. Unigene (EST) derived single nucleotide polymorphism haplotypes of these NILs were aligned with the blast mapping population QTL using Mla as an anchor. Baronesse and all NILs without the 1H introgression were resistant to LB and NB. However, two NILs with the I H introgression were resistant to LB and NB. Both are resistant to stripe rust. Therefore, the QTL conferring resistance to stripe rust is separable by recombination from the blast resistance QTL.

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  • Genetic analysis of seed dormancy QTL in barley

    Kazuhiro Sato, Takashi Matsumoto, Natsuko Ooe, Kazuyoshi Takeda

    BREEDING SCIENCE59 ( 5 ) 645 - 650   2009年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPANESE SOC BREEDING  

    Seed dormancy in wild barley enables drought escape by preventing germination during the hot summer in and environments. Dormancy in cultivated barley has different effects: it can delay the malting process and/or it can prevent pre-harvest sprouting. Thus, cloning dormancy genes in barley will contribute to understanding the domestication process and it will facilitate optimizing the trait for efficient agronomic and industrial uses. Rates of seed germination were used to evaluate dormancy on physiologically matured grain samples that were dried and stored frozen until use. With this phenotypic scoring procedure, many genetic factors controlling seed dormancy has been reported as quantitative trait loci (QTL). Of these QTL, one at the centrometic region of chromosome 5H (Qsd1) has been most frequently identified and shows the largest effect across mapping populations. We also identified this QTL using the EST map based on Haruna Nijo (H. vulgare ssp. vulgare) crossed with wild barley H602 (H. vulgare ssp. spontaneum). We have derived both doubled haploid and recombinant chromosome substitution lines (RCSLs) from this cross. At least four QTLs are segregating in this germplasm. RCSLs having only the Qsd1 segment of wild barley in a Haruna Nijo genetic background were identified and 910 BC3F2 plants were scored for dormancy. In these lines, segregation for dormancy fit a mono-factorial ratio. These germplasm resources are appropriate for map based cloning of Qsd1. Strategies for cloning Qsd1 with these resources are discussed.

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  • An application of high-throughput SNP genotyping for barley genome mapping and characterization of recombinant chromosome substitution lines 査読 国際誌

    Kazuhiro Sato, Kazuyoshi Takeda

    Theor Appl Genet119 ( 4 ) 613 - 9   2009年8月

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  • A high-density transcript linkage map of barley derived from a single population

    K. Sato, N. Nankaku, K. Takeda

    HEREDITY103 ( 2 ) 110 - 117   2009年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    A high-resolution transcript linkage map of barley was created using a single doubled haploid (DH) mapping population, only 3&apos;-end expressed sequence tags ( ESTs) and only PCR-based assays. Cultivar &apos;Haruna Nijo&apos; and an ancestral wild-form accession &apos;H602&apos; were used as EST donors and crossing parents of the mapping population. Of the 10 366 primer sets developed from a non-redundant set of 3&apos;EST sequences, 7700 sets generated useful amplicons and 3975 (52%) showed polymorphisms between the mapping parents. Of these, 2890 (28% of the total) were mapped by single nucleotide polymorphisms ( 1717), cleaved amplified polymorphic sequence ( 933) and INDELs ( 240). The present work involves an estimated 9% of the genes of barley. Of the mapped ESTs, 2689 (93%) are formatted in the Affymetrix Barley 1 GeneChip and full-length cDNA sequences are available for 1039 (36%). Mapped ESTs show highest similarity with sequences in the wheat gene index (93%) and moderate similarity with rice (50%). Comparison of mapped EST positions and the rice pseudomolecule indicated collinear regions between two species; these are particularly conserved for the entire barley chromosome 3H and rice chromosome 1. These mapped genes, together with a systematically developed set of genetic resources, will make it easier to directly clone genes showing simple inheritance and to determine the genetic basis of complex traits. The information will contribute to the development of a framework for the physical mapping of barley, which is necessary for genome sequencing. Heredity ( 2009) 103, 110-117; doi: 10.1038/hdy.2009.57; published online 20 May 2009

    DOI: 10.1038/hdy.2009.57

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  • Development of 5006 full-length CDNAs in barley: a tool for accessing cereal genomics resources. 査読 国際誌

    Kazuhiro Sato, Tadasu Shin-I, Motoaki Seki, Kazuo Shinozaki, Hideya Yoshida, Kazuyoshi Takeda, Yukiko Yamazaki, Matthieu Conte, Yuji Kohara

    DNA research : an international journal for rapid publication of reports on genes and genomes16 ( 2 ) 81 - 9   2009年4月

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    記述言語:英語  

    A collection of 5006 full-length (FL) cDNA sequences was developed in barley. Fifteen mRNA samples from various organs and treatments were pooled to develop a cDNA library using the CAP trapper method. More than 60% of the clones were confirmed to have complete coding sequences, based on comparison with rice amino acid and UniProt sequences. Blastn homologies (E<1E-5) to rice genes and Arabidopsis genes were 89 and 47%, respectively. Of the 5028 possible amino acid sequences derived from the 5006 FLcDNAs, 4032 (80.2%) were classified into 1678 GreenPhyl multigenic families. There were 555 cDNAs showing low homology to both rice and Arabidopsis. Gene ontology annotation by InterProScan indicated that many of these cDNAs (71%) have no known molecular functions and may be unique to barley. The cDNAs showed high homology to Barley 1 GeneChip oligo probes (81%) and the wheat gene index (84%). The high homology between FLcDNAs (27%) and mapped barley expressed sequence tag enabled assigning linkage map positions to 151-233 FLcDNAs on each of the seven barley chromosomes. These comprehensive barley FLcDNAs provide strong platform to connect pre-existing genomic and genetic resources and accelerate gene identification and genome analysis in barley and related species. Sequence data from this article have been deposited with the DDBJ/EMBL/GenBank Data Libraries under accession nos AK248134-AK253139. The online database with annotation is available at http://www.shigen.nig.ac.jp/barley/.

    DOI: 10.1093/dnares/dsn034

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  • Mapping of the eibi1 gene responsible for the drought hypersensitive cuticle in wild barley (Hordeum spontaneum)

    Guoxiong Chen, Takao Komatsudu, Mohammad Pourkheirandish, Mohammad Sameri, Kazuhiro Sato, Tamar Krugman, Tzion Fahima, Abraham B. Korol, Eviatar Nevo

    BREEDING SCIENCE59 ( 1 ) 21 - 26   2009年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPANESE SOC BREEDING  

    Segregation analysis showed that eibi1, a drought hypersensitive Cuticle wild barley mutant, was monogenic and recessive, and mapped in two F, Populations, one made from a cross between the mutant and a Cultivated barley (cv. Morex), and the other between the mutant and another wild barley. A microsatellite marker screen showed that the gene was located oil barley chromosome 3H, and a set of markers already assigned to this chromosome, including both microsatellites and ESTs, was used to construct a genetic map. eibi1 co-segregated with barley EST AV918546, and was located to bin 6. The synteny between barley and rice ill this region is incomplete, with a large discrepancy in map distances, and the presence Of Multiple inversions.

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  • Dissection of barley chromosome 3H in common wheat and a comparison of 3H physical and genetic maps. 査読

    Kazuhiko Sakai, Shuhei Nasuda, Kazuhiro Sato, Takashi R Endo

    Genes & genetic systems84 ( 1 ) 25 - 34   2009年2月

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    記述言語:英語  

    We used the gametocidal system to dissect a barley chromosome 3H added to common wheat. The gametocidal system induced chromosomal structural changes in the 3H addition line of common wheat, and we cytologically screened for rearranged chromosomes involving the 3H chromosome by in situ hybridization (FISH/GISH). We established 50 common wheat lines carrying single rearranged (or dissected) 3H chromosomes of independent origin. The dissected 3H chromosomes were either deletions or translocations with wheat chromosomes, and their breakpoints were in the centromere/the long arm/the short arm in a rough ratio of 1:2:2. We used these so-called 3H dissection lines to map 36 EST markers that were polymorphic between euploid common wheat and the 3H addition line and that had been used for the construction of a 3H genetic map. We conducted PCR analysis to detect the EST markers in the dissection lines. The results of the PCR analysis, which mostly corresponded to the retained or lost segments of the dissected 3H chromosomes, allowed us to place the 36 EST markers into 20 chromosomal regions flanked by the breakpoints of the dissected chromosomes. We compared this physical map constructed in this study with a 3H genetic map constructed using the same EST markers. The order of all EST markers was consistent between the two maps. We briefly discuss on the advantage of the physical mapping using dissection lines over genetic mapping.

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  • Analysis of intraspecies diversity in wheat and barley genomes identifies breakpoints of ancient haplotypes and provides insight in the structure of diploid and hexaploid Triticeae gene pools. 査読 国際誌

    Thomas Wicker, Simon Krattinger, Evans S. Lagudah, Takao Komatsuda, Mohammad Pourk-heirandish, Takashi Matsumoto, Silvie Cloutier, Hiroyuki Kanamori, Kazuhiro Sato, Dragan Perovic, Nils Stein, Beat Keller

    Plant Physiology149 ( 1 ) 258 - 70   2009年1月

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    記述言語:英語  

    DOI: 10.1104/pp.108.129734

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  • The international barley sequencing consortium--at the threshold of efficient access to the barley genome. 査読 国際誌

    Daniela Schulte, Timothy J Close, Andreas Graner, Peter Langridge, Takashi Matsumoto, Gary Muehlbauer, Kazuhiro Sato, Alan H Schulman, Robbie Waugh, Roger P Wise, Nils Stein

    Plant physiology149 ( 1 ) 142 - 7   2009年1月

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    記述言語:英語  

    DOI: 10.1104/pp.108.128967

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  • Identification of novel haze-active beer proteins by proteome analysis

    Takashi Iimure, Nami Nankaku, Megumi Watanabe-Sugimoto, Naohiko Hirota, Zhou Tiansu, Makoto Kihara, Katsuhiro Hayashi, Kazutoshi Ito, Kazuhiro Sato

    JOURNAL OF CEREAL SCIENCE49 ( 1 ) 141 - 147   2009年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD  

    Colloidal haze reduces beer quality considerably. Four haze samples were analyzed by two-dimensional gel electrophoresis (2DE) in order to identify haze-active proteins. Several protein spots were observed in all of the four haze samples. Using mass spectrometry analysis followed by a database search identified these spots as barley dimeric alpha-amylase inhibitor (BDAI-1), CMb component of tetrameric alpha-amylase inhibitor (CMb) and trypsin inhibitor CMe precursor (CMe). These proteins were considered to be haze-active. Since haze-active proteins are adsorbed by silica gel in the beer filtration process, we eluted proteins adsorbed onto silica gel (PAS) and identified their species. These major PAS were identified as protein Z4, protein Z7 and trypsin/amylase inhibitor pUP13 (TAI), rather than BDAI-1, CMb and CMe. Furthermore, we analyzed proline compositions in the beer proteins, PAS and the haze proteins. Consequently, we found that the proline compositions of PAS were higher (ca. 20 mol%) than those in the beer proteins (ca. 10 mol%), although those of the haze-active proteins such as BDAI-1, CMb and CMe were 6.6-8.7 mol%. Our results suggest that BDAI-1, CMb and CMe are not predominant haze-active proteins, but growth factors of beer colloidal haze. (C) 2008 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.jcs.2008.08.004

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  • QTLs in barley controlling seedling elongation of deep-sown seeds

    H. Takahashi, M. Noda, K. Sakurai, A. Watanabe, H. Akagi, K. Sato, K. Takeda

    EUPHYTICA164 ( 3 ) 761 - 768   2008年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    In drought areas, in which topsoil moisture is low, barley seeds are generally sown in the subsoil (deep-seeding). In order for the shoots of germinating seeds to emerge from the deep soil cover, the coleoptile and the first internode of the seedlings must elongate as an adaptive response to deep-seeding. Here, we have mapped quantitative trait loci (QTLs) for these adaptive characteristics. Elongation of the coleoptile and first internode was investigated using seeds sown under two soil cover conditions: at a depth of 9 cm beneath a soil mixture; and, at a depth of 12 cm beneath vermiculite. We identified multiple alleles for increased coleoptile and first internode elongation using a doubled haploid population of 150 lines generated from a cross between the barley cultivars Harrington and TR306. Composite interval mapping analyses of the data revealed two moderate and eleven small effect QTLs, with at least one QTL on each chromosome. The QTLs on chromosomes 5H and 7H had moderate effects on coleoptile elongation (18.5-27.6% of PVE: phenotypic variance explained; 2.6-3.2 mm of Add: additive effect) and first internode elongation (PVE: 16.6-19.6%; Add: 3.1-3.2 mm). The small effect QTLs showed PVEs of less than 15% and an Add range of 1.2-3.2 mm for both characters. A marker assisted selection approach, using markers linked to the QTLs for seedling elongation at deep-seeding, may eventually enable development of drought tolerant barley hybrids.

    DOI: 10.1007/s10681-008-9720-7

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  • Detection of Fusarium head blight resistance QTLs using five populations of top-cross progeny derived from two-row x two-row crosses in barley

    Kazuhiro Sato, Kiyosumi Hori, Kazuyoshi Takeda

    MOLECULAR BREEDING22 ( 4 ) 517 - 526   2008年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Fusarium head blight (FHB) resistance was evaluated in five recombinant inbred (RI) populations. The RI populations consisted of top-cross progeny derived from a diallel set of crosses. Each of five two-row barley lines differing in response to FHB were crossed with 'Harbin 2-row'. FHB severity was scored on an 11-point scale, where resistant = 0 and susceptible = 10, based on the 'cut-spike test'. Disease data were obtained for each population for 2 or 3 years. Linkage maps comprised of expressed sequence tag (EST) markers were developed for each population and used for quantitative trait locus (QTL) detection. Thirty two QTLs were detected using all data sets (individual populations and years). Thirteen QTLs were detected using averages across years; 10 of these were consistent across the individual year and average data sets. These QTLs clustered at 14 regions, with clusters on all chromosomes. At 11 of these clusters, Harbin 2-row contributed FHB resistance alleles. No QTLs were detected near the row type (vrs1) locus in any of the five RI populations, suggesting that the FHB resistance QTL in this region reported in two-row x six-row crosses may be pleiotropic effect of vrs1. QTL were coincident with the flowering type locus (cly1/Cly2) on chromosome 2H in every population. Some QTL x QTL interactions were significant, but these were smaller than QTL main effects. Considering the pleiotropic effect of spike morphology on FHB resistance, future FHB resistance mapping efforts in barley should focus on cross combinations in which alleles at vrs1 are not segregating.

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  • A novel prediction method of beer foam stability using protein Z, barley dimeric alpha amylase inhibitor-I (BDAI-I) and yeast thioredoxin. 査読 国際誌

    Takashi Iimure, Kiyoshi Takoi, Takafumi Kaneko, Makoto Kihara, Katsuhiro Hayashi, Kazutoshi Ito, Kazuhiro Sato, Kazuyoshi Takeda

    J. Agric. Food Chem56 ( 18 ) 8664 - 71   2008年9月

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    記述言語:英語  

    DOI: 10.1021/jf801184k

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  • Barley grain with adhering hulls is controlled by an ERF family transcription factor gene regulating a lipid biosynthesis pathway 査読 国際誌

    S. Taketa, S. Amano, Y. Tsujino, T. Sato, D. Saisho, K. Kakeda, M. Nomura, T. Suzuki, T. Matsumoto, K. Sato, H. Kanamori, S. Kawasaki, K. Takeda

    PNAS105 ( 10 ) 4062 - 7   2008年3月

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  • The influence of barley malt protein modification on beer foam stability and their relationship to the barleydimericr-amylase inhibitor-I (BDAI-I) as a possible foam-promoting protein. 査読 国際誌

    Yoshihiro Okada, Takashi Iimure, Kiyoshi Takoi, Takafumi Kaneko, Makoto Kihara, Katsuhiro Hayashi, Kazutoshi Ito, Kazuhiro Sato, Kazuyoshi Takeda

    J. Agric. Food Chem.56 ( 4 ) 1458 - 64   2008年2月

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    記述言語:英語  

    DOI: 10.1021/jf0724926

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  • Detection of seed dormancy QTL in multiple mapping populations derived from crosses involving novel barley germplasm 査読 国際誌

    Kiyosumi Hori, Kazuhiro Sato, Kazuyoshi Takeda

    Theoretical and Applied Genetics115 ( 6 ) 869 - 76   2007年10月

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  • An Aluminium-activated citrate transporter in barley 査読

    Jun Furukawa, Naoki Yamaji, Hua Wang, Namiki Mitani, Yoshiko Murata, Kazuhiro Sato, Maki Katsuhara, Kazuyoshi Takeda, Jian Feng Ma

    Plant and Cell Physiology48 ( 8 ) 1081 - 91   2007年8月

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    記述言語:英語  

    DOI: 10.1093/pcp/pcm091

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  • Dissection of barley chromosome 5H in common wheat 査読

    Taizo Ashida, Shuhei Nasuda, Kazuhiro Sato, Takashi R. Endo

    Genes and Genetic Systems82 ( 2 ) 123 - 33   2007年4月

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    記述言語:英語  

    DOI: 10.1266/ggs.82.123

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  • Construction and characterization of a bacterial artificial chromosome (BAC) library for Japanese malting barley ‘Haruna Nijo’

    Daisuke Saisho, Eriko Myoraku, Shinji Kawasaki, Kazuhiro Sato, Kazuyoshi Takeda

    Breeding Science57 ( 1 ) 29 - 38   2007年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Linkage Map Construction and QTL Detection Based on Barley ESTs in A genome Diploid Wheat

    Kiyosumi Hori, Sayuri Takehara, Nami Nankaku, Kazuhiro Sato, Tetsuo Sasakuma, Kazuyo-shi Takeda

    Breeding Science57   39 - 45   2007年

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  • Barley EST markers enhance map saturation and QTL mapping in diploid wheat

    Kiyosumi Hori, Sayuri Takehara, Nami Nankaku, Kazuhiro Sato, Tetsuo Sasakuma, Kazuyoshi Takeda

    Breeding Science57 ( 1 ) 39 - 45   2007年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A linkage map was constructed using PCR-based markers developed from barley expressed sequence tags (ESTs) for recombinant inbred (RI) lines (F 10) derived from a cross between Triticum monococcum L. strain KT3-5 and T. boeoticum Boiss. strain KT1-1. Among the primer sets of 2,695 barley ESTs, 242 EST markers were integrated into the frame of a previously constructed diploid wheat linkage map with 96 RFLP markers. The total map length comprising barley EST, previously mapped RFLP and morphological markers was 1,038.1 cM, and the average distance between two adjacent loci was 3.1 cM. The linkage map showed saturation with the barley EST markers by covering extended telomeric regions and increasing the resolution compared with the previous RFLP map. Order of barley ESTs on the diploid wheat linkage map mostly showed a collinearity to that on the barley linkage map constructed previously, although the long arms of the homoeologous linkage groups 4 and 5 showed structural changes between diploid wheat and barley. Phenotypes of nine quantitative traits were scored for the diploid wheat RI lines and their parents. Composite interval mapping enabled to detect 24 quantitative trait loci for the traits. Thus, barley ESTs could become powerful tools to reveal homoeologous relationships between barley and wheat chromosomes, and valuable resources to develop genetic markers for wheat genomes.

    DOI: 10.1270/jsbbs.57.39

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  • Genomic distribution of MITEs in barley determined by MITE-AFLP mapping

    H. Takahashi, H. Akagi, K. Mori, K. Sato, K. Takeda

    GENOME49 ( 12 ) 1616 - 1620   2006年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS  

    Miniature inverted-repeat transposable elements (MITES) represent a large superfamily of transposons that are moderately to highly repetitive and frequently found near or within plant genes. To elucidate the organization of MITES in the barley genome, MITES were integrated into the genetic map of barley. In this report, we describe the use of MITES in amplified fragment length polymorphism (AFLP) mapping, and demonstrate their superiority over conventional AFLP mapping. Barley MITES include members of the Stowaway, Barfly, and Pangrangja families. By amplifying the flanking sequences of these MITES, a total of 214 loci were mapped from a population of 93 doubled-haploid segregating individuals between Hordeum vulgare ssp. vulgare and H. vulgare ssp. spontaneum. The 214 MITE-AFLP and 40 anchor simple sequence repeat (SSR) loci were distributed on 7 linkage groups, covering a total map distance of 1 165 cM. The average marker density on each chromosome ranged between 3.4 and 9.6 cM per locus. Only 1 MITE-based locus was frequently found to be associated with MITE loci from the same family, resulting in clusters in chromosomal subregions. In barley, it will be possible to cover the entire genome with a limited set of MITE-based primers and to build highly dense maps of specific regions.

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  • Mapping multiple disease resistance genes using a barley mapping population evaluated in Peru, Mexico, and the USA

    C. Rossi, A. Cuesta-Marcos, I. Vales, L. Gomez-Pando, G. Orjeda, R. Wise, K. Sato, K. Hori, F. Capettini, H. Vivar, X. Chen, P. Hayes

    MOLECULAR BREEDING18 ( 4 ) 355 - 366   2006年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    We used a well-characterized barley mapping population (BCD 47 x Baronesse) to determine if barley stripe rust (BSR) resistance quantitative trait loci (QTL) mapped in Mexico and the USA were effective against a reported new race in Peru. Essentially the same resistance QTL were detected using data from each of the three environments, indicating that these resistance alleles are effective against the spectrum of naturally occurring races at these sites. In addition to the mapping population, we evaluated a germplasm array consisting of lines with different numbers of mapped BSR resistance alleles. A higher BSR disease severity on CI10587, which has a single qualitative resistance gene, in Peru versus Mexico suggests there are differences in pathogen virulence between the two locations. Confirmation of a new race in Peru will require characterization using a standard set of differentials, an experiment that is underway. The highest levels of resistance in Peru were observed when the qualitative resistance gene was pyramided with quantitative resistance alleles. We also used the mapping population to locate QTL conferring resistance to barley leaf rust and barley powdery mildew. For mildew, we identified resistance QTL under field conditions in Peru that are distinct from the Mla resistance that we mapped using specific isolates under controlled conditions. These results demonstrate the long-term utility of a reference mapping population and a well-characterized germplasm array for locating and validating genes conferring quantitative and qualitative resistance to multiple pathogens.

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  • RMo1 confers blast resistance in barley and is located within the complex of resistance genes containing Mla, a powdery mildew resistance gene. 査読 国際誌

    Tsuyoshi Inukai, M Isabel Vales, Kiyosumi Hori, Kazuhiro Sato, Patrick M Hayes

    Molecular plant-microbe interactions : MPMI19 ( 9 ) 1034 - 41   2006年9月

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    記述言語:英語  

    Isolates of Magnaporthe oryzae (the causal agent of rice blast disease) can infect a range of grass species, including barley. We report that barley Hordeum vulgare cv. Baronesse and an experimental line, BCD47, show a range of resistance reactions to infection with two rice blast isolates. The complete resistance of Baronesse to the isolate Ken 54-20 is controlled by a single dominant gene, designated RMo1. RMo1 mapped to the same linkage map position on chromosome 1H as the powdery mildew resistance locus Mla and an expressed sequence tag (k04320) that corresponds to the barley gene 711N16.16. A resistance quantitative trait locus (QTL), at which Baronesse contributed the resistance allele, to the isolate Ken 53-33 also mapped at the same position as RMo1. Synteny analysis revealed that a corresponding region on rice chromosome 5 includes the bacterial blight resistance gene xa5. These results indicate that a defined region on the short arm of barley chromosome 1H, including RMo1 and Mla, harbors genes conferring qualitative and quantitative resistance to multiple pathogens. The partial resistance of BCD47 to Ken53-33 is determined by alleles at three QTL, two of which coincide with the linkage map positions of the mildew resistance genes mlo and Mlf.

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  • An atlas of gene expression from seed to seed through barley development 査読 国際誌

    Arnis Druka, Kazuhiro Sato ほか

    Functional & Integrative Genomics6 ( 3 ) 202 - 11   2006年7月

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  • QTL analysis of fusarium head blight severity in recombinant inbred population derived from a cross between two-rowed barley varieties

    K Hori, K Sato, T Kobayashi, K Takeda

    BREEDING SCIENCE56 ( 1 ) 25 - 30   2006年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPANESE SOC BREEDING  

    Fusarium head blight (FHB) severity was evaluated in a set of recombinant inbred (RI) lines from a cross between the two-rowed barley varieties, Harbin 2-row (resistant) and Turkey 6 (susceptible). Harbin 2-row was one of the most resistant varieties to FHB among ca. 5,000 barley germplasm accessions stored at Okayama University. The FHB severity of the RI lines and their parents was evaluated by the 'cut-spike test' in the 2000-2001 and 2003-2004 seasons. Spikes with disease symptoms were scored based on eleven grades from resistant (0) to susceptible (10). A high-density linkage map, including 328 AFLP, 45 SSR, one RFLP-STS, one AFLP-STS, four resistance gene analog (RGA) and four morphological markers, was constructed to detect QTLs for FHB severity. Composite interval mapping enabled to detect one QTL on chromosome 2H in the 2000-2001 season, and one QTL on chromosome 4H and another QTL on chromosome 6H in the 2003-2004 season. At these three QTLs, the alleles from Harbin 2-row contributed to a lower severity. A QTL oil chromosome 2H was located in the vicinity of the cleistogamy locus (cly1 or Cly2) that determines open/closed flowering type. The comparison with previously identified QTLs for FHB severity by the present authors indicated that Harbin 2-row might harbor the same resistance allele in the vicinity of the cly1/Cly2 locus as Russia 6, which was used as a resistant parent in the previous study. The QTL in the vicinity of the cly1/Cly2 locus is one of the promising sources for FHB resistance breeding in barley.

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  • RMo1 conferring blast resistance is located within the complex of resistance genes containing powdery mildew resistance locus Mla on chromosome 1H in barley

    T. Inukai, M. I. Vales, K. Hori, K. Sato, P. M. Hayes

    Molecular Plant Microbe Interactions   2006年

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  • QTL analysis of Fusarium head blight resistance using a high-density linkage map in barley

    K Hori, T Kobayashi, K Sato, K Takeda

    THEORETICAL AND APPLIED GENETICS111 ( 8 ) 1661 - 1672   2005年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Fusarium head blight (FHB) resistance was evaluated in a set of recombinant inbred (RI) lines from a cross between Russia 6 (resistant) and H.E.S. 4 (susceptible), which had one of the widest differences of FHB resistance reactions among ca. 5,000 barley germplasm accessions in Okayama University. Field-grown spikes were sampled and inoculated by the 'cut-spike test'. Resistance reactions on the parents and RI lines were scored by eleven grades, from resistant (0) to susceptible (10). Quantitative trait loci (QTL) analysis detected three QTL: two located on the long arm of chromosome 2H, and another on the short arm of chromosome 5H. A QTL located on chromosome 2H was coincident with the vrs1 locus, which governs inflorescence row type. The other QTL on chromosome 2H was positioned in the vicinity of cleistogamy locus (cly1 or Cly2) that determines inflorescence opening/closing. Resistant gene analog (RGA) and expressed sequence tag (EST) markers with homology for disease resistance genes were integrated into the high-density linkage map. Most of these markers were not localized near the identified resistance QTL, except for one RGA marker (FXLRRfor_XLRRrev170) localized in the vicinity of the cly1/Cly2 locus. Five AFLP markers localized in the vicinity of the identified QTL were sequenced to convert them into sequence tagged site (STS) markers. Genotyping of each RI line using two AFLP-STS markers and the vrs1 locus indicated that the RI lines with three Russia 6 QTL alleles exhibited the same level of high FHB resistance reactions as Russia 6. In contrast, RI lines with three susceptible alleles showed reactions close to H.E.S. 4. Therefore, the markers closely linked to the QTL can be efficiently used for the selection of resistance.

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  • QTL analysis in recombinant chromosome substitution lines and doubled haploid lines derived from a cross between Hordeum vulgare ssp vulgare and Hordeum vulgare ssp spontaneum

    K Hori, K Sato, N Nankaku, K Takeda

    MOLECULAR BREEDING16 ( 4 ) 295 - 311   2005年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Recombinant chromosome substitution lines (RCSLs) were developed in BC3 generation to introduce segments of a wild barley strain 'H602' (Hordeum vulgare ssp. spontaneum) into a barley cultivar 'Haruna Nijo' (H. vulgare ssp. vulgare) genetic background. One hundred thirty four RCSLs were genotyped by 25 SSR and 60 EST markers, which were localized on a linkage map of doubled haploid lines (DHLs) derived from the same cross combination. Graphical genotyping revealed that the observed average substitution ratio of H602 segment (12.9%) agreed with the expected substitution ratio (12.5%), and a minimum set of 19 RCSLs represented the entire H602 genome. Phenotypes of five qualitative and nine quantitative traits were scored in both the RCSLs and DHLs. Five qualitative traits were localized as morphological markers on the linkage map of the DHLs, and these molecular markers were aligned on the respective chromosomal regions in the RCSLs. Simple and composite interval mapping procedures detected a total of 18 and 24 QTLs for nine qualitative traits on the RCSLs and DHLs, respectively. Several QTLs were localized at coincident or very close regions on both linkage maps. In spite of general inferior agronomic performances in wild barley, several H602 QTL alleles showed agronomically positive effects. These RCSLs should contribute to substitution of favorable alleles from wild barley into cultivated barley. These RCSLs are also available as sources of near isogenic lines, with which we can apply advanced genetic analysis methods such as isolation of QTLs and detection of epistatic interactions among QTLs.

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  • Chromosomal assignment and deletion mapping of barley EST markers. 査読

    Shuhei Nasuda, Yukari Kikkawa, Taizo Ashida, A K M Rafiqul Islam, Kazuhiro Sato, Takashi R Endo

    Genes & genetic systems80 ( 5 ) 357 - 66   2005年10月

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    記述言語:英語  

    From about 10000 PCR-based EST markers of barley we chose 1421 EST markers that were demonstrated to be amplified differently by PCR between wheat (Triticum aestivum cv. Chinese Spring) and barley (Hordeum vulgare cv. Betzes). We assigned them to the seven barley chromosomes (1H to 7H) by PCR analysis using a set of wheat-barley chromosome addition lines. We successfully assigned 701 (49.3%) EST markers to the barley chromosomes: 75 to 1H, 127 to 2H, 119 to 3H, 94 to 4H, 108 to 5H, 81 to 6H and 97 to 7H. By using a set of Betzes barley telosomic addition lines of Chinese Spring, we could successfully determine the chromosome-arm (S or L) location of at least 90% of the EST markers assigned to each barley chromosome. We conducted a trial mapping using 90 EST markers assigned to 7HS (49) or 7HL (41) and 19 wheat lines carrying 7H structural changes. More EST markers were found in the distal region than in the proximal region.

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  • Genomic differentiaion of Hordeum chilense from H. vulgare as revealed by repetitive and EST sequences 査読

    A. A. Hagras, M. Kishii, H. Tanaka, K. Sato, H. Tsujimoto

    Genes and Ge-netic Systems80 ( 3 ) 147 - 59   2005年6月

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    記述言語:英語  

    DOI: 10.1266/ggs.80.147

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  • Chromosomal assignment and deletion mapping of barley EST markers

    Shuhei Nasuda, Yukari Kikkawa, Taizo Ashida, A. K. M. Rafiqul Islam, Kazuhiro Sato, Takashi R. Endo

    Genes and Genetic Systems80 ( 5 ) 357 - 366   2005年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    From about 10000 PCR-based EST markers of barley we chose 1421 EST markers that were demonstrated to be amplified differently by PCR between wheat (Triticum aestivum cv. Chinese Spring) and barley (Hordeum vulgare cv. Betzes). We assigned them to the seven barley chromosomes (1H to 7H) by PCR analysis using a set of wheat-barley chromosome addition lines. We successfully assigned 701 (49.3%) EST markers to the barley chromosomes: 75 to 1H, 127 to 2H, 119 to 3H, 94 to 4H, 108 to 5H, 81 to 6H and 97 to 7H. By using a set of Betzes barley telosomic addition lines of Chinese Spring, we could successfully determine the chromosome-arm (S or L) location of at least 90% of the EST markers assigned to each barley chromosome. We conducted a trial mapping using 90 EST markers assigned to 7HS (49) or 7HL (41) and 19 wheat lines carrying 7H structural changes. More EST markers were found in the distal region than in the proximal region.

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  • Extended application of barley EST markers for the analysis of alien chromosomes added to wheat genetic background 査読

    Adel Abdel-Aziz Hagras, Masahiro Kishii, Kazuhiro Sato, Hiroyuki Tanaka, Hisashi Tsujimoto

    Breeding Science55 ( 3 ) 335 - 341   2005年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The main objective of this study is to produce DNA markers of polymorphism between wheat and some important alien species. The applicability of 1,165 barley EST primer sets to amplify markers showing polymorphism between wheat and ten alien species, covering a wide range of variation in Triticeae, was investigated. These primers consisted of four series. Series 1 was randomly chosen from a pool of barley EST primer sets. The remaining three were pre-screened in a previous study and showed polymorphic and co-amplified patterns between barley and wheat. From 22% to 100% of the primer sets amplified single clear bands in the species
    from 29% to 75% of which were polymorphic to wheat. The frequency of amplification corresponded with their phylogenetic distance from barley. Many DNA markers (78-859) showing polymorphism between each species and wheat were obtained. These markers are expected to be valuable in identifying the alien chromosomes added to a wheat genetic background. The usefulness of the markers in the basic and the applied studies of the wild species is discussed.

    DOI: 10.1270/jsbbs.55.335

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  • Extended application of barley EST markers for the genomic analysis of wheat and barley-related species

    A. A. Hagras, M. Kishii, K. Sato, H. Tanaka, H. Tsujimoto

    Breed. Sci.   2005年

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  • Genetic variation in component traits of heading date in Hordeum vulgare subsp spontaneum accessions characterized in controlled environments

    Karsai, I, PM Hayes, J Kling, IA Matus, K Meszaros, L Lang, Z Bedo, K Sato

    CROP SCIENCE44 ( 5 ) 1622 - 1632   2004年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CROP SCIENCE SOC AMER  

    Ancestral germplasm may be a useful source of genetic variation for crop improvement. Genetic variation in developmental traits that contribute to heading date may be useful in developing varieties that are uniquely tailored to specific stress environments. Hordeum vulgare subsp. spontaneum (K. Koch) A. & Gr. is the ancestor of cultivated barley and useful developmental trait alleles may have been lost in the domestication process. Accordingly, we surveyed a sample of 16 subsp. spontaneum accessions for vernalization requirement, photoperiod sensitivity, photoperiod response, and relative earliness. We compared the subsp. spontaneum accessions to four H. vulgare L. subsp. vulgare accessions representing spring, facultative, and winter growth habit. Thirteen subsp. spontaneum accessions originating from the Fertile Crescent and the one subsp. spontaneum accession from the Caucasus region required vernalization; they were responsive to long photoperiods and most were very early. Two subsp. spontaneum accessions from the Himalayan region had no vernalization requirement but were extremely sensitive to short photoperiods. We used a clustering procedure to define two groups of subsp. spontaneum accessions, a group of subsp. spontaneum that included the two subsp. vulgare accessions of spring and facultative growth habit, and a fourth group comprised of the two subsp. vulgare winter habit cultivars. These data indicate that subsp. spontaneum may be a source of novel alleles for growth habit.

    DOI: 10.2135/cropsci2004.1622

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  • Molecular mapping of a gene responsible for Al-activated secretion of citrate in barley. 査読 国際誌

    J. F. Ma, S. Nagao, K. Sato, H. Ito, J. Furukawa, K. Takeda

    J. Exp. Bot55 ( 401 ) 1335 - 41   2004年6月

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    記述言語:英語  

    DOI: 10.1093/jxb/erh152

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  • Development and characterization of recombinant chromosome substitution lines (RCSLs) using Hordeum vulgare subsp. spontaneum as a source of donot alles in a Hor-deum vulgare subsp. vulgare background.

    I. Matus, A. Corey, T. Filichkin, P. M. Hayes, I. Vales, J. Kling, O. Riera, K. Sato, W. Powell, R. Waugh

    Genome   2004年

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  • Differential Al resistance and citrate secretion in barley (Hordeum vulgare L.) 査読 国際誌

    Z. Zhao, J. Ma, K. Sato, K. Takeda

    Planta217 ( 5 ) 794 - 800   2003年9月

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    記述言語:英語  

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  • Efficient construction of high-density linkage map and its application to QTL analysis in barley

    K Hori, T Kobayashi, A Shimizu, K Sato, K Takeda, S Kawasaki

    THEORETICAL AND APPLIED GENETICS107 ( 5 ) 806 - 813   2003年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER-VERLAG  

    Using a High Efficiency Genome Scanning (HEGS) system and recombinant inbred (RI) lines derived from the cross of Russia 6 and H.E.S. 4, a high-density genetic map was constructed in barley. The resulting 1,595.7-cM map encompassed 1,172 loci distributed on the seven linkage groups comprising 1,134 AFLP, 34 SSR, three STS and vrs1 (kernel row type) loci. Including PCR reactions, gel electrophoresis and data processing, 6 months of work by a single person was sufficient for the whole mapping procedure under a reasonable cost. To make an appraisal of the resolution of genetic analysis for the 95 RI lines based on the constructed linkage map, we measured three agronomic traits: plant height, spike exsertion length and 1,000-kernel weight, and the analyzed quantitative trait loci (QTLs) associated with these traits. The results were compared on the number of detected QTLs and their effects between a high-density map and a skeleton map constructed by selected AFLP and anchor markers. The composite interval mapping on the high-density map detected more QTLs than the other analyses. Closely linked markers with QTLs on the high-density map could be powerful tools for marker-assisted selection in barley breeding programs and further genetic analyses including an advanced backcross analysis or a map-based cloning of QTL.

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  • A root-specific O-methyltransferase gene expressed in salt-tolerant barley 査読 国際誌

    M. Sugimoto, Y. Okada, K. Sato, K. Ito, K. Takeda

    Biosci. Biotechnol. Biochem.67 ( 5 ) 966 - 72   2003年5月

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    記述言語:英語  

    DOI: 10.1271/bbb.67.966

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  • Identification of AFLP and microsatellite markers linked with an aluminium tolerance gene in barley (Hordeum vulgare L.)

    H Raman, JS Moroni, K Sato, BJ Read, BJ Scott

    THEORETICAL AND APPLIED GENETICS105 ( 2-3 ) 458 - 464   2002年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER-VERLAG  

    Barley is the most sensitive among the cereals to aluminium (Al) stress and breeding for more tolerant cultivars is a priority. To enhance selection efficiency for Al tolerance in barley, PCR-based AFLP and microsatellite markers linked to a locus conferring tolerance to aluminium were identified. The study used F-2 progeny derived from a single cross between Yambla (moderately tolerant of Al) and WB229 (tolerant of Al) and developed hydroponic pulse-recovery screening methods to assess tolerance of phenotypes based on root growth. The segregation ratios of tolerant and sensitive genotypes and F-3 progeny testing suggest that a single major gene controlled Al tolerance (Alt). In order to determine the chromosomal location of the Alt gene, we used the AFLP technique coupled with bulk segregant analysis. We evaluated tolerant and sensitive bulks using 30 combinations of EcoRI/MseI primers, and 12 of these permitted differentiation of the sensitive and tolerant bulks. More than 1,000 amplified fragments were obtained, and 98 polymorphic bands were scored. AFLP analysis of wheat-barley chromosome addition lines indicated that the Alt gene was located on barley chromosome 4H. Four chromosome 4H-specific microsatellite markers (Bmac310, Bmag353, HVM68 and HVMCABG) were tightly linked to Alt. The large allelic variation detected with microsatellite marker Bmag353 allowed us to implement this marker for routine marker-assisted selection for Al tolerance, and 396 plants could be screened on a single gel.

    DOI: 10.1007/s00122-002-0934-0

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  • QTL analysis of agronomic traits in barley based on the doubled haploid progeny of two elite North American varieties representing different germplasm groups

    LA Marquez-Cedillo, PM Hayes, A Kleinhofs, WG Legge, BG Rossnagel, K Sato, SE Ullrich, DM Wesenberg

    THEORETICAL AND APPLIED GENETICS103 ( 4 ) 625 - 637   2001年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER-VERLAG  

    A better understanding of the genetics of complex traits, such as yield, may be achieved by using molecular tools. This study was conducted to estimate the number, genome location, effect and allele phase of QTLs determining agronomic traits in the two North American matting barley (Hordeum vulgare L.) quality variety standards. Using a doubled haploid population of 140 lines from the cross of two-rowed Harrington x sixrowed Morex, agronomic phenotypic data sets from nine environments, and a 107-marker linkage map, we performed QTL analyses using simple interval mapping and simplified composite interval mapping procedures. Thirty-five QTLs were associated, either across environments or in individual environments, with five grain and agronomic traits (yield, kernel plumpness, test weight, heading date, and plant height). Significant QTL x environment interaction was detected for all traits. These interactions resulted from both changes in the magnitude of response and changes in the sign of the allelic effect. QTLs for multiple traits were coincident. The vrs1 locus on chromosome 2 (2H), which determines inflorescence row type, was coincident with the largest-effect QTL determining four traits (yield, kernel plumpness, test weight, and plant height). QTL analyses were also conducted separately for each sub-population (six-rowed and two-rowed). Seven new QTLs were detected in the sub-populations. Positive transgressive segregants were found for all traits, but they were more prevalent in the six-rowed sub-population. QTL analysis should be useful for identifying candidate genes and introgressing favorable alleles between germplasm groups.

    DOI: 10.1007/PL00002919

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  • Mapping of quantitative trait loci conferring resistance to barley yellow mosaic virus in a Chinese barley landrace Mokusekko 3

    C Miyazaki, E Osanai, K Saeki, K Ito, T Konishi, K Sato, A Saito

    BREEDING SCIENCE51 ( 3 ) 171 - 177   2001年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPANESE SOC BREEDING  

    A Chinese six-rowed barley landrace, Mokusekko 3, exhibits a wide resistance spectrum to all the barley yellow mosaic virus strains in Japan (BaYMV-I, -II, -III) and in Europe (BaYMV-1, -2) as well as to barley mild mosaic virus (BaMMV). To identify the genetic basis for the resistance to BaYMV, a cross between a Japanese malting barley cultivar Ko A (susceptible) and Mokusekko 3 was made to generate F-2 and F-3 populations. The reaction of the F-2-derived F-3 lines to BaYMV was investigated in a field infected with BaYMV (-I or both -I and -II) during two growing seasons, 1996 and 1997. BaYMV disease severity of the F-2 individuals was quantitatively expressed as the percentage of the susceptible seedlings within the F3 line. For the linkage analysis along the whole chromosome, a map of the barley genome consisting of 100 markers was used. The map covers 1291 cM with an average density of 14 cM. The interval mapping method was used to identify the quantitative trait loci (QTLs) for resistance to BaYMV. A new QTL for resistance to the BaYMV was located in the terminal region of chromosome 7HS, explaining 20-25 % of the phenotypic variance, in addition to those in the terminal region of chromosome 3HL and centromeric region of chromosome 4H. The three QTLs accounted for 55-57 % of the total phenotypic variance. The location of the QTLs on chromosomes 4H and 3HL corresponded well to that of rym1 and rym5 which were previously mapped for resistance to BaYMV in Mokusekko 3 using morphological and isozyme markers. Based on these results, the effect of pyramiding of these resistance factors into modern barley cultivars. by marker-assisted selection is was examined.

    DOI: 10.1270/jsbbs.51.171

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  • Molecular mapping of the Oregon Wolfe Barleys: a phenotypically polymorphic doubled-haploid population

    JM Costa, A Corey, PM Hayes, C Jobet, A Kleinhofs, A Kopisch-Obusch, SF Kramer, D Kudrna, M Li, O Riera-Lizarazu, K Sato, P Szucs, T Toojinda, MI Vales, RI Wolfe

    THEORETICAL AND APPLIED GENETICS103 ( 2-3 ) 415 - 424   2001年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER-VERLAG  

    A phenotypically polymorphic barley (Hordeum vulgare L.) mapping population was developed using morphological marker stocks as parents. Ninety-four doubled-haploid lines were derived for genetic mapping from an F-1 using the Hordeum bulbosum system. A linkage map was constructed using 12 morphological markers, 87 restriction fragment length polymorphism (RFLP), five random amplified polymorphic DNA (RAPD), one sequence-tagged site (STS), one intron fragment length polymorphism (IFLP), 33 simple sequence repeat (SSR), and 586 amplified fragment length polymorphism (AFLP) markers. The genetic map spanned 1,387 cM with an average density of one marker every 1.9 cM. AFLP markers tended to cluster on centromeric regions and were more abundant on chromosome 1 (7H). RAPD markers showed a high level of segregation distortion, 54% compared with the 26% observed for AFLP markers, 27% for SSR markers, and 18% for RFLP markers. Three major regions of segregation distortion, based on RFLP and morphological markers, were located on chromosomes 2 (2H), 3 (3H), and 7 (5H). Segregation distortion may indicate that preferential gametic selection occurred during the development of the doubled-haploid lines. This may be due to the extreme phenotypes determined by alleles at morphological trait loci of the dominant and recessive parental stocks. Several molecular markers were found to be closely linked to morphological loci. The linkage map reported herein will be useful in integrating data on quantitative traits with morphological variants and should aid in map-based cloning of genes controlling morphological traits.

    DOI: 10.1007/s001220100622

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  • QTL analysis of resistance to the rice blast pathogen in barley (Hordeum vulgare)

    K Sato, T Inukai, PM Hayes

    THEORETICAL AND APPLIED GENETICS102 ( 6-7 ) 916 - 920   2001年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER-VERLAG  

    Barley is compatible with the rice blast pathogen (Pyricularia oryzae Cav.). Fiftyfour barley cultivars of diverse geographic origin and pedigree were inoculated with three isolates of the rice blast pathogen. All barley genotypes showed blast disease symptoms when inoculated at the seedling stage with each of the three isolates. However, one genotype showed quantitative resistance to all three isolates and three genotypes showed quantitative resistance to one or two of the isolates. By inoculating a set of doubled-haploid lines derived from the cross 'Harrington' (susceptible) and 'TR306' (resistant) with isolate Ken 54-20, we mapped quantitative trait loci (QTLs) determining seedling stage blast resistance. At all QTLs, TR306 contributed the resistance alleles. The four QTLs, when considered jointly, explained 43.6% of the phenotypic variation in blast symptom expression. A comparison of the blast resistance QTLs with other disease resistance QTLs reported in this population revealed a region on chromosome 4 (4H) with multiple disease resistance loci. It will be useful to capitalize on the syntenic relationship of rice and barley and to integrate information on species-specific resistance genes with information on the reaction of the two species to the same pathogen.

    DOI: 10.1007/s001220000507

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  • Mapping genes for deep-seeding tolerance in barley

    H Takahashi, K Sato, K Takeda

    EUPHYTICA122 ( 1 ) 37 - 43   2001年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KLUWER ACADEMIC PUBL  

    Deep-seeding tolerance, the emergence of seedlings from deep seeded conditions, is involved in stand establishment in semi-arid regions, where the soil surface is too dry for seed germination. Genes determining deep-seeding tolerance in barley were mapped using two doubled haploid populations derived from the following crosses: Harrington x TR306 (H/T) and Steptoe x Morex (S/M). Significant quantitative trait loci (QTLs) for deep-seeding tolerance were found in each population. Two QTLs explained 40% of the phenotypic variation in the H/T population and one QTL (S/M) 8% of the total phenotypic variance. Multiple QTLs accounting for coleoptile length and first internode length were detected in both populations. In the H/T population, there were coincident QTLs for deep-seeding tolerance, coleoptile length and first internode length on the long arm of chromosome 5H. These QTLs correspond with previously reported QTLs for abscisic acid and gibberellic acid response. QTL coincidence may be due to the pleiotropic effects of alleles at a single locus. This information may be useful for breeding programs manipulating morphological and physiological traits in order to develop varieties for semi-arid regions.

    DOI: 10.1023/A:1012608721291

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  • QTL analysis of malting quality in barley based on the doubled-haploid progeny of two elite North American varieties representing different germplasm groups

    LA Marquez-Cedillo, PM Hayes, BL Jones, A Kleinhofs, WG Legge, BG Rossnagel, K Sato, E Ullrich, DM Wesenberg

    THEORETICAL AND APPLIED GENETICS101 ( 1-2 ) 173 - 184   2000年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER-VERLAG  

    Characterization of the determinants of economically important phenotypes showing complex inheritance should lead to the more effective use of genetic resources. This study was conducted to determine the number, genome location and effects of QTLs determining malting quality in the two North American barley quality standards. Using a doubled-haploid population of 140 lines from the cross of HarringtonxMorex, malting quality phenotype data sets from eight environments, and a 107-marker linkage map, QTL analyses were performed using simple interval mapping and simplified composite interval mapping procedures. Seventeen QTLs were associated with seven grain and malting quality traits (percentage of plump kernels, test weight, grain protein percentage, soluble/total protein ratio, alpha-amylase activity, diastatic power and malt-extract percentage). QTLs for multiple traits were coincident. The loci controlling inflorescence type [vrs1 on chromosome 2(2H) and int-c on chromosome 4(4H)] were coincident with QTLs affecting all traits except malt-extract percentage. The largest effect QTLs, for the percentage of plump kernels, test weight protein percentage, S/T ratio and diastatic power, were coincident with the vrs1 locus. QTL analyses were conducted separately for each sub-population (six-rowed and two-rowed). Eleven new QTLs were detected in the subpopulations. There were significant interactions between the vrs1 and int-c loci for grain-protein percentage and S/T protein ratio. Results suggest that this mating of two different germplasm groups caused a disruption of the balance of traits. Information on the number, position and effects of QTLs determining components of malting quality may be useful for maintaining specific allele configurations that determine target quality profiles.

    DOI: 10.1007/s001220051466

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  • Inheritance of gramine content in barley

    S Moharramipour, K Takeda, K Sato, H Yoshida, H Tsumuki

    EUPHYTICA106 ( 2 ) 181 - 185   1999年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KLUWER ACADEMIC PUBL  

    The inheritance of gramine indole alkaloid was studied in F-1 and F-2 generations derived from crosses between wild (Hordeum vulgare subsp. spontaneum) and cultivated (H. vulgare subsp. vulgare) barley at adult stage in the field. The means of gramine content were very low in F(1)s and F(2)s in April and May. The ratio of progeny containing gramine to those without gramine (near zero or undetectable) fit a mono-genic or di-genic model in F-2 generation. However, the content of gramine might be controlled by minor genes in the gramine-containing plants. The direction of dominance was toward lower gramine content. The averages of dominance (h/d) in F-1 and F-2 ranged from partial to complete. Broad-sense heritabilities were high (0.70 to 0.77). The usefulness of hybrids for breeding aphid resistance and possibility increasing palatability of grazing pasture for sheep and cattle is discussed.

    DOI: 10.1023/A:1003535823329

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  • Inheritance of resistance to cereal aphids in the eross of wild and cultivated barley

    SABRAO J. of Breeding & Genetics31   33 - 41   1998年

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  • Mapping resistance to cereal aphids in barley

    S Moharramipour, H Tsumuki, K Sato, H Yoshida

    THEORETICAL AND APPLIED GENETICS94 ( 5 ) 592 - 596   1997年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER VERLAG  

    A set of 150 doubled-haploid (DH) barley (Hordeum vulgare L.) lines derived from the cross of Harrington/TR306 was used to determine the number and chromosomal location of quantitative trait loci (QTLs) controlling resistance to cereal aphids. The experiments were conducted under natural infestation in the field during two growing seasons: 1994 and 1995. Aphid resistance was measured by counting the number of aphids per plot. Counts were made on a weekly basis. Each year at the time of maximum aphid density there was an obvious difference in reaction between the parental genotypes. The DH lines showed continuous variation for aphid density. Simple interval mapping and simplified composite interval mapping revealed that the principal QTL determining cereal aphid resistance is on the distal region of the short arm of chromosome 1. This aphid-resistance QTL is linked with a heading-date QTL. At the time of highest aphid infestation, this QTL accounted for 31% and 22% of the total variance of aphid density in 1994 and 1995, respectively. A QTL on chromosome 5 was also detected but only by simplified composite interval mapping. However, the largest consistent effect was due to the QTL on the short arm of chromosome 1. This QTL may be a useful target for marker-assisted selection for adult plant cereal aphid resistance in barley.

    DOI: 10.1007/s001220050455

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  • Effects of leaf color, epicuticular wax amount and gramine content in barley hybrids on cereal aphid populations

    S Moharramipour, H Tsumuki, K Sato, S Murata, K Kanehisa

    APPLIED ENTOMOLOGY AND ZOOLOGY32 ( 1 ) 1 - 8   1997年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC APPL ENTOMOL ZOOL  

    Using four marker genotypes of F-2 hybrids derived from crosses between aphid resistant and susceptible barley lines, the effects of leaf color, epicuticular wax and gramine indole alkaloid on aphid infestation were studied in natural populations of cereal aphids, mainly the corn leaf aphid (Rhopalosiphum maidis) during 1994-1995. Resistant lines, OUH603 and OUH689 (Hordeum vulgare subsp. spontaneum), were the normal type (green waxy) with a higher amount of gramine. However, a susceptible line, OUL117 (Hordeum vulgare subsp. vulgare), was the yellow waxless type with a lower content of gramine. In the F-2 populations of OUL117 x OUH689, OUH603 x OUL117 and OUL117 x OUH603, the aphid densities on the marker genotypes were low in the normal type, intermediate in the green waxless and yellow waxy types, and high in the yellow waxless type. Gramine content was low and did not differ significantly among the genotypes measured in 158 F-2 plants of OUL117 x OUH689 in 1994. Additionally, there was no significant correlation between aphid density and gramine content within each genotype in the F-2 populations. These results showed that the genes controlling color and wax types had additive effects on normal plant genotype resistance to aphids, but did not affect gramine content.

    DOI: 10.1303/aez.32.1

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  • Chracterizing and exploiting genetic diversity and quantitative traits in barley(Hordeum vulgare)using AFLP markers.

    J. Quantitative Trait Loci 2   1997年

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  • Net blotch resistance in wild species of Hordeum

    K Sato, K Takeda

    EUPHYTICA95 ( 2 ) 179 - 185   1997年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KLUWER ACADEMIC PUBL  

    Resistance to net blotch was evaluated in 175 Hordeum vulgare subsp. spontaneum (H. spontaneum) accessions and 149 accessions of thirteen species or subspecies of wild Hordeum. Most H. spontaneum accessions showed resistance to each of the four Pyrenophora teres f. teres (P. teres) isolates tested. However, H. spontaneum accessions showed different resistance reactions, depending upon their origin. In particular, some accessions from Afghanistan and Russia showed a high level of resistance, and accessions from Morocco were susceptible. Among the four P. teres isolates, the virulence spectra on the H. spontaneum accessions were more different between isolates from different countries than between those from the same country. Hordeum spontaneum accessions susceptible to the Canadian isolate WRS102, but resistant to the other three isolates were found in Iraq suggesting the geographical differentiation of resistance genes in H. spontaneum. All accessions of the other wild Hordeum species, especially some accessions of H. marinum subsp. gussoneanum, showed high levels of resistance. These resistance genes may be useful candidates for incorporation into cultivated barley.

    DOI: 10.1023/A:1002958924439

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  • Mapping genes for callus growth and shoot regeneration in barley (Hordeum vulgare L)

    Y Mano, H Takahashi, K Sato, K Takeda

    BREEDING SCIENCE46 ( 2 ) 137 - 142   1996年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPANESE SOC BREEDING  

    The quantitative trait loci (QTLs) controlling callus growth and subsequent shoot regeneration were identified by 222 markers in doubled haploid (DH) lines derived from the cross between Steptoe x Morex in barley (Hordeum vulgare L.). The DH lines showed a wide and continuous variation in these two tissue culture traits, and the transgressive segregation was observed for the shoot regeneration rate among the DH lines, The interval mapping analysis revealed that callus growth and shoot regeneration ability were controlled by multiple genes and the position of individual genes was different each other, Morex contributed two QTLs for callus growth tentatively named Qcg 1 and Qcg 2, Steptoe contributed three QTLs for shoot regeneration named Qsr 1, Qsr 2, and Qsr 3, while Morex contributed a QTL for shoot regeneration named Qsr 4, In the multiple genic model, two QTLs for callus growth and four QTLs for shoot regeneration accounted for 47.8 % and 49.8 % of the total variation in the population, respectively, These findings indicated that lines with high regeneration ability could be selected by pyramiding the multiple genes controlling high regeneration ability.

    DOI: 10.1270/jsbbs1951.46.137

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  • Mapping genes for callus growth and shoot regeneration in barley (Hordeum vulgare L.).

    MANO Y, TAKAHASHI H, SATO K, TAKEDA K

    Breed Sci46: 137-142 ( 2 ) 137 - 142   1996年6月

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  • EFFECT OF THE DWARFING GENE FROM DEE-GEO-WOO-GEN ON CULM AND INTERNODE LENGTHS, AND ITS RESPONSE TO FERTILIZER IN RICE

    M MURAI, N SHINBASHI, S SATO, K SATO, H ARAKI, M EHARA

    BREEDING SCIENCE45 ( 1 ) 7 - 14   1995年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPANESE SOC BREEDING  

    The effect of the dwarfing gene from Dee-geo-woo-gen (indicated by d-47 in this study) on culm and internode lengths was investigated. Following isogenic dwarf lines were used : the two isogenic lines of d-47 of Shiokari and Taichung 65 (abbreviated to d-47 line and d-47(T65) line, respectively); and Calrose 76 (abbreviated to C76) which contains sd-1 at the same locus as d-47. Three F-1's between the three dwarf lines and their respective parental cultivars, and two kinds of F-2 populations were used. Two and three fertilizer levels were set for d-47 line and Shiokari, and d-47(T65) line and Taichung 65, respectively. The difference between d-47(T65) line and Taichung 65 in culm length was 28.0 cm under a condition of basal dressing, and the difference was 27 % of the culm length of Taichung 65. Those between d-47 line and Shiokari were 27.0 cm and 38 %. Those between C76 and Calrose were 20.0 cm and 19 %. The difference in internode length between each of dwarf lines and its parental cultivar became smaller from the first to 6th internodes. However, the percentage of the difference to the value of each of the parental cultivars in the first (the upper) internode length was lower than those in the third to fifth (the lower) internode lengths. Therefore, it was evident that d-47 and sd-1 act to change toward the ''Upper internode elongation type''. The three genotypes distributed in the order of d-47 /d-47&lt; d-47 /+ &lt; +/+ with respect to culm length in each of the F-2's of d-47(T65) line x Taichung 65 and Shiokari x d-47 line. The culm length of each of the three F-1's was between those of its dwarf and tall parents. Similar tendencies were shown in the first to third internode lengths. These facts indicated the incomplete recessiveness of d-47. The lengths of culm and the first to fifth internodes of d-27(T65) line, d-47 line and their parental cultivars increased with increase of fertilizer. The increases in d-47(T65) line and d47 line were smaller than those in their respective parental cultivars. It was inferred that d-47 exerts its positive effect on lodging resistance at a high fertilizer level rather than at a low fertilizer level.

    DOI: 10.1270/jsbbs1951.45.7

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  • ビールオオムギにおける穀皮歩合のダイアレル分析

    育種学雑誌39 ( 4 )   1989年

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  • The barley genome

    Stein, Nils, Muehlbauer, Gary J

    Springer  2018年  ( ISBN:9783319925271

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    総ページ数:xviii, 394 p.   記述言語:英語

    CiNii Books

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  • Genetic diversity and germplasm management- wild barley, landraces, breeding materials. Kumlehn, J. and Stein, N. eds. Biotechnological Approaches to Barley Improvement. Biotechnology in Agriculture and Forestry

    Springer  2014年 

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  • 植物の遺伝と育種第2版

    朝倉書店  2013年 

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  • Beer and Wort Proteomics. In PLANT PROTEOMICS METHODS AND PROTOCOLS

    Springer Science  2013年 

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  • オオムギの分類・起源・伝播.

    朝倉書店  2011年 

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  • 品種改良の世界史. オオムギ.

    悠書館  2011年 

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  • History of breeding. Barley

    2011年 

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  • Classification, origin and disbribution of barley

    2011年 

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  • Genome Analysis: The State of Knowledge of Barley Genes. In Barley: Improvement, Production, and Uses.

    John Wiley and Sons, Inc., New York  2011年 

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  • 植物ゲノム科学辞典

    朝倉書店  2009年 

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  • 植物育種学事典

    培風館  2005年 

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  • 農学大辞典第3版

    養賢堂  2004年 

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  • Diversity in barley (Hordeum vulgare)

    Elsevier Science, The Netherlands.  2003年 

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  • Diversity in barley (Hordeum vulgare)

    Elsevier Science, The Netherlands  2003年 

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  • Diversity in barley (Hordeum vulgare)

    Elsevier Science, The Netherlands.  2003年 

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  • Diversity in barley (Hordeum vulgare)

    Elsevier Science, The Netherlands.  2003年 

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  • Diversity in barley (Hordeum vulgare)

    Elsevier Science, The Netherlands  2003年 

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  • Diversity in barley (Hordeum vulgare)

    Elsevier Science, The Netherlands.  2003年 

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  • 植物育種学

    文英堂,東京  2003年 

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  • Diversity in barley : Hordeum vulgare

    Bothmer, Roland von, Hintum, Theo van, Knüpffer, Helmut, 佐藤, 和広

    Elsevier Science  2003年  ( ISBN:0444505857

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    総ページ数:xvii, 280 p.   記述言語:英語

    CiNii Books

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  • ライフサイエンスのための系統保存とデータバンク

    共立出版  2000年 

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  • 植物のゲノムサイエンスプロトコール-オオムギ-.

    秀潤社  2000年 

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MISC

  • 醸造用オオムギの一穂粒数増大に向けた同質遺伝子系統の育成

    最相大輔, 轟貴智, 原口雄飛, 甲斐浩臣, 半田裕一, 佐藤和広

    育種学研究21   33   2019年9月

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    記述言語:日本語  

    J-GLOBAL

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  • 塩ストレス下でのオオムギの花粉稔性低下に関わる遺伝子の探索

    児玉明日香, 成田亮平, 山口真功, 久野裕, 安達俊輔, 高木宏樹, 平沢正, 佐藤和広, 大川泰一郎

    育種学研究21   23   2019年3月

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    記述言語:日本語  

    J-GLOBAL

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  • バイオリソースと育種学のこれからを考える:NBRPリソースの活用事例から

    那須田 周平, 伊藤 瑛海, 佐藤 豊, 久野 裕, 佐藤 和広, 小松田 隆夫, 石川 亮, 橋口 正嗣, 鈴木 章弘, 星川 健

    育種学研究21 ( 1 ) 81 - 85   2019年

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    記述言語:日本語   出版者・発行元:日本育種学会  

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  • パンコムギにおけるCRISPR/Cas9を用いたTaQsd1全同祖遺伝子のゲノム編集 1:編集ホモ系統の効率的な作出

    安倍史高, 久野裕, 三上雅史, 三上雅史, 神谷容子, 大西一光, HAQUE E, 田中剛, 遠藤真咲, 川浦香奈子, 佐藤和広

    育種学研究20   88   2018年9月

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  • パンコムギにおけるCRISPR/Cas9を用いたTaQsd1全同祖遺伝子のゲノム編集:3.編集ホモ系統の形質評価

    HAQUE E, 安倍史高, 林武司, 久野裕, 田中剛, 神谷容子, 川浦香奈子, 佐藤和広

    育種学研究20   162   2018年9月

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  • パンコムギにおけるCRISPR/Cas9を用いたTaQsd1全同祖遺伝子のゲノム編集 2:導入遺伝子の分離除去

    神谷容子, 川浦香奈子, 安倍史高, 田中剛, HAQUE E, 三上雅史, 三上雅史, 遠藤真咲, 久野裕, 佐藤和広

    育種学研究20   89   2018年9月

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  • 塩ストレス条件で稔実歩合を高く維持するオオムギの量的形質遺伝子座(QTL)の同定

    児玉明日香, 成田亮平, 山口真功, 久野裕, 安達俊輔, 高木宏樹, 大川泰一郎, 佐藤和広, 平沢正

    日本作物学会講演会要旨集245th   125   2018年3月

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  • ムギ類に共通する有用遺伝子の構造と機能.

    佐藤和広, 小松田隆夫

    育種学研究20   53 - 58   2018年

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  • パンコムギにおけるCRISPR/Cas9システムを用いたゲノム編集用ターゲット配列の評価系の確立

    神谷容子, 安倍史高, 久野裕, 佐藤和広, 三上雅史, 三上雅史, 遠藤真咲, 川浦香奈子

    育種学研究19   96   2017年10月

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  • 世界初、オオムギ穂発芽耐性遺伝子SD2の特定 招待

    中村信吾, Mohammad Pourkheirandish, 森重弘美, 久保佑太, 中村雅子, 市村和也, 瀬尾茂美, 金森裕之, 呉健忠, 安藤露, Goetz Hensel, Mohammad Sameri, Nils Stein, 佐藤和広, 松本隆, 矢野昌裕, 小松田隆夫

    NARO 成果情報 2016   2016年12月

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    記述言語:日本語   掲載種別:機関テクニカルレポート,技術報告書,プレプリント等  

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  • コムギ6B染色体上の赤さび病抵抗性遺伝子LrRW12領域のゲノム構造解析

    小林史典, 水野信之, 田中剛, 金森裕之, 片寄裕一, WU J, 佐藤和広, 那須田周平, 半田裕一

    育種学研究18   220   2016年9月

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  • オオムギの休眠を制御する新たな仕組みを発見 -降雨による収穫前の発芽防止が可能に- 招待

    佐藤和広, 小松田隆夫, 中村信吾

    岡山大学 プレスリリース   2016年5月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

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  • 長期間の塩ストレス条件におけるオオムギの花粉稔性の品種間差の解析

    児玉明日香, SAGE Tammy L, BAGHA Shaheen, 安達俊輔, 大川泰一郎, 佐藤和広, 平沢正

    日本作物学会講演会要旨集241st   98   2016年3月

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  • オオムギ発芽時耐塩性のQTL解析

    伊藤大樹, 最相大輔, 本庄三恵, 八杉公基, 永野惇, 高萩航太郎, 持田恵一, 佐藤和広

    育種学研究17   236   2015年3月

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  • 麦類の種子休眠制御遺伝子の解析

    中村信吾, POURKHEIRANDISH Mohammad, 森重弘美, 久保佑太, 中村雅子, 市村和也, 川東広幸, HENZEL Goetz, SAMERI Mohammad, 安倍史高, 佐藤和広, 金森裕之, 呉健忠, 松本隆, 矢野昌裕, 小松田隆夫

    日本植物生理学会年会要旨集55th   115   2014年3月

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  • 育種学教育の現状と展望.

    佐藤和広, 福井希一

    育種学研究15   134 - 138   2013年

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  • オオムギのゲノムとビールのプロテオーム

    佐藤和広, 飯牟礼隆

    Foods and Food Ingredients Journal of Japan   2013年

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  • オオムギゲノム多様性の解析と育種への応用.

    佐藤和広

    育種学研究15   167 - 172   2013年

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  • オオムギ完全長cDNA 24,783配列をデータベースから公開

    松本隆, 田中剛, 坂井寛章, 金森裕之, 栗田加奈子, 堀清純, 伊藤剛, 佐藤和広

    農業生物資源研究所主な研究成果2011   4 - 5   2012年6月

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  • オオムギの各種いもち病菌抵抗性に関与する複合遺伝子座Rmo2の作用解析と分子マッピング

    HYON G.‐S, NGUYEN Thi, Thanh Nga, 井上喜博, 中馬いづみ, 麻野北斗, 佐藤和広, 土佐幸男

    日本植物病理学会報78 ( 1 ) 38   2012年2月

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  • 11-11 オオムギのアルミニウム耐性機構の獲得(11.植物の有害元素,2012年度鳥取大会)

    柏野 美帆, 横正 健剛, 山地 直樹, 最相 大輔, 山根 美樹, 高橋 宏和, 佐藤 和広, 中園 幹生, 馬 建鋒

    日本土壌肥料学会講演要旨集58 ( 0 )   2012年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • 栽培オオムギにおける春化要求性の自然変異:高度秋播性は東アジアに偏在する

    最相大輔, 石井誠, 堀清純, 佐藤和広

    育種学研究13   80   2011年9月

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  • 22,651のオオムギ完全長cDNA配列の作成と機能解析

    田中剛, 松本隆, 坂井寛章, 天野直己, 金森裕之, 栗田加奈子, 菊田有里, 神谷梢, 山本麻裕, 伊川浩司, 藤井信之, 堀清純, 伊藤剛, 佐藤和広

    日本植物生理学会年会要旨集52nd   250   2011年3月

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  • オオムギの各種いもち病菌抵抗性に関与する複合遺伝子座Rmo2の同定と分子マッピング

    HYON G, NGUYEN Thi, Thanh Nga, 中馬いづみ, 岡田和馬, 佐藤和広, 土佐幸雄

    日本植物病理学会大会プログラム・講演要旨予稿集2011   52   2011年3月

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  • 11-16 オオムギアルミニウム活性型クエン酸トランスポーター遺伝子HvAACT1の発現調節機構(11.植物の有害元素,2010年度北海道大会)

    藤井 美帆, 山地 直樹, 中園 幹生, 佐藤 和広, 馬 建鋒

    日本土壌肥料学会講演要旨集 ( 56 )   2010年9月

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    記述言語:日本語   出版者・発行元:一般社団法人日本土壌肥料学会  

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  • Wheat and Barley.

    Takeda K, Yoshino H, Enomoto T, Long C, Smekalova T, Kato K, Sato K, Tsujimoto H, Tsuyuzaki H, Tanaka H, Nishida H

    Genetic assay and study of crop germplasm in and around China (4th)   21 - 59   2010年

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  • オオムギ その多様性とゲノムソース

    佐藤和広

    細胞工学別冊   2009年

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  • オオムギの多様性とゲノムをとらえるリソース

    佐藤和広

    細胞工学26 ( 2 ) 203 - 204   2007年

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  • 9-6 オオムギにおけるアルミニウムによるクエン酸分泌に関与する遺伝子の単離と機能解析(9.植物の無機栄養,2007年度東京大会)

    古川 純, 山地 直樹, 王 華, 三谷 奈見季, 村田 佳子, 佐藤 和広, 且原 真木, 武田 和義, 馬 建鋒

    日本土壌肥料学会講演要旨集53 ( 0 )   2007年

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    記述言語:日本語   出版者・発行元:一般社団法人 日本土壌肥料学会  

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  • オオムギの多様性とゲノムをとらえるリソース

    細胞工学26 ( 2 ) 203 - 204   2007年

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  • オオムギにおけるSNPsの育種への応用

    佐藤和広

    植物の生育調節42   61 - 73   2007年

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  • Wheat and Barley.

    Takeda K, Yoshino H, Enomoto T, Kato K, Sato K, Tsujimoto H, Tsuyuzaki H, Tanaka H

    Genetic assay and study of crop germplasm in and around China (3rd)   35 - 68   2006年

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  • Bioresource in barley-diversity and genome-

    K Sato, K Takeda

    PLANT AND CELL PHYSIOLOGY47   S254 - S254   2006年

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:OXFORD UNIV PRESS  

    Web of Science

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  • Transcriptiomic analysis of gene expression pattern in the root tips of Al-tolerant and -sensitive cultivars of barley

    J Furukawa, JF Ma, K Sato, K Takeda

    PLANT AND CELL PHYSIOLOGY47   S121 - S121   2006年

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:OXFORD UNIV PRESS  

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  • Introduction of genetic diversity into cereals from their wild relatives

    Sato K, Tsujimoto H, Bothmer R. von

    Proceedings of the 5th International Triticeae Symposium, Praugh, Czech Republic, June 6th -10th, 2005   103 - 105   2005年

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)  

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  • Genotypic variation in Fe concentration of barley grain

    HF Ma, A Higashitani, K Sato, K Takeda

    SOIL SCIENCE AND PLANT NUTRITION50 ( 7 ) 1115 - 1117   2004年12月

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    記述言語:英語   出版者・発行元:TAYLOR & FRANCIS LTD  

    Iron deficiency in humans is the most prevalent micronutrient deficiency worldwide. To screen for high Fe cultivar, genotypic variation in Fe concentration of barley grain was investigated in two collections of barley: 274 standard varieties selected at the Barley Germplasm Center of the Research Institute for Bioresources, Okayama University (SV) and 135 varieties from the Barley Core Collection of Americas (BCCAM). The Fe concentration of barley grain showed large variation, ranging from 24.6 to 63.3 mg kg(-1) in SV, from 21.0 to 83.0 mg kg(-1) in BCCAM barleys. The Fe concentration was not affected by the key characters of barley varieties, kernel row types, and hulled or hull-less. The Fe concentration was also not related to the place of origin of the barley variety. About 90% of total Fe was localized in the grain without hull. These results provide fundamental data for breeding Fe-rich cultivars and for studying the mechanisms involved in genotypic variation in Fe concentration.

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  • Towards understanding whole feature of Fusarium head blight resistance in Triticeae: A new approach using barley EST markers

    sujimoto H, Hagras A, Kishii M, Hori K, Sato K, Takeda K

    Proceedings of the JIRCAS workshop on collaboration research for Fusarium head blight resistance in wheat and barley   38 - 41   2004年

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)  

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  • イネの鉄耐性,オオムギ赤かび病抵抗性の高精度・高能率QTL分析

    清水顕史, 堀清純, 佐藤和広, 武田和義, 谷坂俊隆, 川崎信二

    農業生物資源研究所主要な研究成果2002   74 - 75   2003年5月

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  • Genotypic variation in silicon concentration of barley grain

    JF Ma, A Higashitani, K Sato, K Takeda

    PLANT AND SOIL249 ( 2 ) 383 - 387   2003年2月

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    記述言語:英語   出版者・発行元:KLUWER ACADEMIC PUBL  

    Soluble silicon (Si) in foods and drinks has been suggested to have a protective effect against neurotoxicity of Al. We investigated the genotypic variation in Si concentration of barley grain, which has many uses including in livestock feeds, malts for beer and whisky, and some foods for human consumption. Two collections of barley, grown in the same field, were subjected to analysis; 274 standard varieties selected at the Barley Germplasm Center of the Research Institute for Bioresources, Okayama University (SV), and 135 varieties from the Barley Core Collection of Americans (BCCAM). The Si concentration of barley grain showed large variation, ranging from 0 (under detection) to 3600 mg kg(-1) in SV and from 0 to 3800 mg kg(-1) in BCCAM barleys. The Si concentration was much lower in hull-less barley than in hulled barley. The Si concentration of two-row barley was similar to that of six-row barley, suggesting that Si concentration is not affected by the number of spike rows. Si concentration also did not differ with the origin of the barley variety. More than 80% of total Si was localized in the hull. The Si concentration of the hull was between 15 343 and 27 089 mg kg(-1) in the varieties tested. A close correlation was obtained between the Si concentrations of barley grains harvested in different years, suggesting that the variation in Si concentration of barley grain is controlled genetically. These results provide fundamental data for breeding Si-rich cultivars.

    DOI: 10.1023/A:1022842421926

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  • A new secretion pattern of citrate is triggered by Al in barley

    ZQ Zhao, JF Ma, K Sato, K Takeda

    PLANT AND CELL PHYSIOLOGY44   S18 - S18   2003年

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:OXFORD UNIV PRESS  

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  • イネおよびオオムギにおける細胞増殖因子(PSK)遺伝子のマッピング

    高橋 秀和, 赤木 宏守, 森 宏一, 花井 秀俊, 佐藤 和広, 武田 和義

    育種学研究 = Breeding research4   244 - 244   2002年8月

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    記述言語:日本語  

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  • 高密度連鎖地図を用いたオオムギ赤カビ病抵抗性のQTL解析

    堀 清純, 小林 哲朗, 佐藤 和広, 武田 和義, 川崎 信二

    育種学研究 = Breeding research4   2002年8月

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    記述言語:日本語  

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  • 塩ストレス抵抗性オオムギに関する研究 : 2. 塩ストレスで誘導されるO-methyltransferase遺伝子のクローニングと構造

    杉本 学, 岡田 吉弘, 佐藤 和広, 伊藤 一敏, 武田 和義

    育種学研究 = Breeding research4   2002年8月

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    記述言語:日本語  

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  • HEGSによるイネとオオムギの高密度マップの短期作製

    川崎信二, 清水顕史, 谷坂隆俊, 堀清純, 佐藤和広, 武田和義

    農業生物資源研究所主要な研究成果2001   62 - 63   2002年5月

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  • 塩ストレス抵抗性オオムギに関する研究1. 塩ストレスで特異的に発現する遺伝子の解析

    杉本 学, 且原 真木, 岡田 吉弘, 佐藤 和広, 伊藤 一敏, 武田 和義

    育種学研究 = Breeding research4 ( 1 )   2002年3月

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    記述言語:日本語  

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  • 高能率ゲノム走査法(HEGS)によるオオムギ高密度連鎖地図の短期作成

    堀 清純, 小林 哲朗, 清水 顕史, 佐藤 和広, 武田 和義, 川崎 信二

    育種学研究 = Breeding research4 ( 1 )   2002年3月

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  • Wheat and Barley.

    Takeda K, Kato K, Sato K, Tsujimoto H, Tsuyuzaki H

    Towards an understanding of rice, soybean, wheat and barley germplasm in   50 - 86   1997年

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  • Diallel cross analysis of characters in barley.

    Korean J. Life Science   1997年

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  • オオムギにおけるQTL解析とネットワークの利用

    佐藤和広

    計量生物学17   79 - 90   1996年

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  • 野生オオムギ Hordeum spontaneum C.Koch におけるうどんこ病抵抗性の変異.

    岡山大学資源生物科学研究所報報告2   1994年

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  • Numerical taxonomic studies in annual cyperaceous weeds.

    Res. Inst. Bioresour. , Okayama Univ. 2   1994年

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  • Pathogenic variation of Pyrenophora teres isolates collected from Japanese and Canadian spring barley

      1 ( 2 ) 147 - 158   1994年

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  • Selection Effectiveness for the Resistance to Net Blotch in Barley

    Bull. Res. Inst. Bioresour. Okayama Univ.3 ( 1 ) 43 - 53   1994年

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  • Numerical taxonomic studies in annual cyperaceous weeds.

    Res. Inst. Bioresour. , Okayama Univ. 2   1994年

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  • Pathogenic variation of Pyrenophora teres isolates collected from Japanese and Canadian spring barley

    Bull. Res.Inst. Bioresour., Okayama Univ.1 ( 2 ) 147 - 158   1994年

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  • Sources of Resistance to Net Blotch in Barley Germplasm

    Res. Inst. Bioresour., Okayama Univ.2 ( 1 ) 91 - 102   1994年

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  • Selection Effectiveness for the Resistance to Net Blotch in Barley

    Bull. Res. Inst. Bioresour. Okayama Univ.3 ( 1 ) 43 - 53   1994年

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  • オオムギの葉の剛毛に関する遺伝子分析

    岡山大学資源生物科学研究所報告2 ( 1 ) 63 - 68   1994年

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  • 大麦網斑病における幼苗検定法の確立と抵抗性品種の検索

    岡山大学資源生物科学研究所報告1 ( 1 )   1992年

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  • 大麦網斑病菌(Pyrenophora teres Drechs.)の分生子形成に関する研究II.近紫外光照射下における日長培地および温度の効果

    農学研究62 ( 3 )   1991年

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  • 大麦網斑病菌(Pyrenophora teres Drechs.)の分生子形成に関する研究I.温度,培地および光質の効果

    農学研究62 ( 2 )   1990年

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▼全件表示

講演・口頭発表等

  • コムギ準等質遺伝子系統を用いたRNA-seq によるNe1 とNe2 染色体領域の検出

    ムギ類研究会  2017年 

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  • パンコムギにおける CRISPR/Cas9 システムを用いたゲノム編集用ターゲット配列の評価系の確立

    日本育種学会講演会  2017年 

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  • 中国青海省チベット高原で採集した野生コムギ連遺伝資源の遺伝学的特徴付け

    日本育種学会講演会  2017年 

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  • オオムギ種子休眠性 DNA マーカーの簡易検出法

    日本育種学会講演会  2017年 

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  • オオムギの形質転換効率に関わる遺伝子単離に向けた DNA マーカー開発

    日本育種学会講演会  2017年 

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  • オオムギの変わりものと私たちの食卓

    日本遺伝学会公開市民講座  2017年 

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  • オオムギ遺伝資源における圃場出穂期の年次変動

    日本育種学会講演会  2017年 

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  • Baby boom1 ならびに Wuschel2 に制御されるオオムギ遺伝子の単離

    日本育種学会講演会  2017年 

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  • RNA-seq によるSitopsis 節5 種のゲノムワイド多型の検出

    ムギ類研究会  2017年 

     詳細を見る

  • オオムギを用いた澱粉粒の形についての研究

    ムギ類研究会  2017年 

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  • RNA-seq による一粒系コムギのゲノムワイド多型の検出

    ムギ類研究会  2017年 

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  • 醸造品種の農業形質改良に向けた日本の在来品種「早木曽 2 号」の染色体部分置換系統群の育成

    日本育種学会講演会  2017年 

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  • Structure and function of a major seed dormancy gene in barley.

    日本育種学会講演会  2017年 

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  • ビールの泡品質を良くするためのプロテオーム解析とそのビール大麦育種への応用

    日本醸造学会講演会  2017年 

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  • オオムギの形質転換効率に関する遺伝学的解析

    中国地域育種談話会  2017年 

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  • オオムギの形質転換能に関わるゲノム領域の同定

    分子生物学会,  2017年 

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  • 実験用オオムギの栽培

    ムギ類研究会  2017年 

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  • 精麦特性向上に寄与する大麦破砕澱粉粒(far)形質 -変異遺伝子同定と選抜マーカー開発

    ムギ類研究会  2017年 

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  • 二粒系コムギのクロリナ変異NILsのRNA-seq解析

    ムギ類研究会  2016年 

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  • オオムギにおけるカルス特異的発現遺伝子のプロモーター単離

    日本育種学会講演会  2016年 

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  • 中国青海省チベット高原におけるムギ類遺伝資源の探索及び収集.

    日本育種学会講演会  2016年 

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  • RNA-seq 解析によるコムギ近縁 U ゲノム種Aegilops umbellulata のゲノムワイドな多型検出

    日本育種学会講演会  2016年 

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  • RNA-seq データに基づいた合成パンコムギ D ゲノム上の特定領域へのタルホコムギ scaffolds の補充

    日本育種学会講演会  2016年 

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  • オオムギ育種用 SNP タイピングシステムの開発

    日本育種学会講演会  2016年 

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  • 長期間の塩ストレス条件におけるオオムギの不稔歩合の品種間差の解析

    日本作物学会講演会  2016年 

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  • オオムギのα-アミラーゼ活性に関するQTL解析

    日本育種学会中国地区談話会  2016年 

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  • RNA-Seq解析を利用した日本に分布するチガヤの遺伝的分化の解明

    日本雑草学会講演会  2016年 

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  • Identification of the genomic region responding to amenability of Agrobacterium-mediated transformation in barley

    12th International Barley Genetics Symposium  2016年 

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  • Isolation of seed dormancy QTL Qsd1 in barley

    12th International Barley Genetics Symposium  2016年 

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  • National Bioresource Project –Barley- Resources to access barley genome diversity

    8th International Meeting of Asian Network of Research Resource Centers  2016年 

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  • オオムギの形質転換効率に関わるゲノム領域の特定

    日本植物細胞分子生物学会  2016年 

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  • 形質転換可能なオオムギ系統を作る方法

    日本育種学会講演会  2016年 

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  • RNA-seq によるライムギミジェット染色体遺伝子

    日本育種学会講演会  2016年 

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  • オオムギ種子休眠性 QTLQsd1 の単離と機能解析

    日本育種学会講演会  2016年 

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  • コムギ 6B 染色体上の赤さび病抵抗性遺伝子 LrRW12領域のゲノム構造解析

    日本育種学会講演会  2016年 

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  • オオムギの萎縮病抵抗性に関する QTL 解析

    日本育種学会講演会  2016年 

     詳細を見る

  • オオムギのゲノム情報

    ムギ類研究会  2016年 

     詳細を見る

  • 合成パンコムギへのRNA-seq-based BSAの適用

    ムギ類研究会  2016年 

     詳細を見る

  • Aegilops umbellulataゲノムワイド多型情報のマッピングへの利用

    ムギ類研究会  2016年 

     詳細を見る

  • 中国西海省チベット高原におけるムギ類遺伝資源の収集.

    日本育種学会中国地区談話会  2015年 

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  • オオムギ RNA-Seq に基づく多型検出とマーカー作成.

    日本育種学会講演会  2015年 

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  • RNA-seq 解析による6倍体コムギの同祖遺伝子間の発現パターン比較.

    日本育種学会講演会  2015年 

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  • The backup copy of barley seed at the Svalbard Global Seed Vault.

    7th Asian Network of Research Resource Centers meeting  2015年 

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  • QTL-seqを用いたオオムギ黒穎遺伝子座のマッピング.

    麦類研究会  2015年 

     詳細を見る

  • オオムギアルファアミラーゼのゲノムワイド関連解析(予報).

    麦類研究会  2015年 

     詳細を見る

  • NBRPオオムギのリソースとその利活用.

    麦類研究会  2015年 

     詳細を見る

  • RNA-Seq解析によるオオムギ品種間SNPsとマーカー開発.

    麦類研究会  2015年 

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  • オオムギ網斑病抵抗性遺伝子のマッピング.

    日本育種学会中国地区談話会  2015年 

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  • オオムギアルファアミラーゼの品種変異と関連解析.

    日本育種学会中国地区談話会  2015年 

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  • Exome-capture を用いたオオムギ黒頴遺伝子のマッピング.

    日本育種学会講演会  2015年 

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  • マップベースクローニングによるオオムギ種子休眠 QTL SD2 の原因遺伝子の単離.

    日本育種学会講演会  2015年 

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  • RNA-Seq 解析により開発したオオムギ育種用マーカー候補.

    日本育種学会講演会  2015年 

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  • 塩ストレス誘導遺伝子を過剰発現したオオムギ形質転換体の作出.

    日本育種学会講演会  2015年 

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  • オオムギ発芽時耐塩性の QTL 解析.

    日本育種学会講演会  2015年 

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  • 二倍体野生コムギ2種の種間交雑により生じる異常種子の RNAseq 解析.

    日本育種学会講演会  2015年 

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  • Fine-mapping of Rmo2, a resistance locus in barley against the blast fngus.

    日本植物病理学会大会  2015年 

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  • オオムギ種子休眠性Qsd1の同定と解析

    穂発芽研究会  2015年 

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  • オオムギの形質転換に必要なゲノム領域の同定.

    日本育種学会講演会  2015年 

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  • タルホコムギの葉と幼穂の RNA-seq データに基づく選択的スプライシング産物の検出.

    日本育種学会講演会  2015年 

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  • タルホコムギ 10 系統の幼苗葉の RNA-seq データからの SNP の検出.

    日本育種学会講演会  2015年 

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  • はるな二条のゲノム配列によるオオムギ遺伝子情報の高度化.

    日本育種学会講演会  2015年 

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  • オオムギ黒頴遺伝子座(Blp)のマッピング

    中国地区育種談話会  2014年 

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  • 旧ソ連邦地域における祖先型野生オオムギの収集

    日本育種学会講演会  2014年 

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  • キルギスにおけるムギ類遺伝資源の探索及び収集

    日本育種学会講演会  2014年 

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  • 形質転換によるオオムギ種子の皮裸性決定遺伝子Nudの機能証明

    日本育種学会講演会  2014年 

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  • 栽培オオムギのアルミニウム耐性がもたらす東アジアへの適応分化

    日本育種学会講演会  2014年 

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  • Association mapping for salinity tolerance in barley. Annual Meeting of Japanese Society of Breeding

    日本育種学会講演会  2014年 

     詳細を見る

  • マイクロアレイによるオオムギカルスの網羅的な遺伝子発現解析. 日本育種学会講演会

    日本育種学会講演会  2014年 

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  • 塩ストレス条件下におけるオオムギの稔実歩合の量的形質遺伝子座 (QTL) 解析

    日本作物学会講演会  2014年 

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  • オオムギのRNA-seq解析によるゲノム育種用マーカーの開発

    ムギ類研究会  2014年 

     詳細を見る

  • ニコチンアミドモノヌクレオチドによる植物の病害抵抗性誘導の解析

    植物病理学会大会  2014年 

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  • 赤かび病抵抗性及びかび毒感受性に関わるUBQ/RPS27a遺伝子の機能解析

    植物病理学会大会  2014年 

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  • オオムギのストレス耐性遺伝子

    ガンマーフィールドシンポジウム  2014年 

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  • オオムギ育種用マーカーデータベースの開発

    日本育種学会講演会  2014年 

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  • RNA-seq解析によるパンコムギ品種間の多型検出

    日本育種学会講演会  2014年 

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  • オオムギゲノム育種のためのマーカーデータベース構築

    中国地区育種談話会  2014年 

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  • 中央アジアにおける麦類遺伝資源の収集.

    日本育種学会四国談話会  2013年 

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  • Genetic and genomic resources of Barley.

    Japan-Turkey-Afghanistan collaboration workshop for “Planning Meeting of Germplasm Conservation and Utilization for Re-establishing the National Gene Bank system in Afghanistan”  2013年 

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  • オオムギゲノム多様性の解析と育種への応用

    日本育種学会講演会  2013年 

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  • 「ミカモゴールデン」と「Harrington」のオオムギ倍加半数体集団を用いた高密度連鎖地図の構築と麦芽品質のQTL解析.

    日本育種学会講演会  2013年 

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  • 一粒系コムギ早生突然変異系統はPHYTOCLOCK 1 遺伝子のコムギホモログを欠失している.

    日本育種学会講演会  2013年 

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  • 北コーカサスで採集されたタルホコムギのジェノタイピング.

    日本育種学会講演会  2013年 

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  • Barley natural variation and adaptation to global environments.

    16th Australian Barley Technical Symposium  2013年 

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  • Barley as a model of Triticeae genome.

    Triticeae Cytogenetics: Past, Present and Future  2013年 

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  • Barley enters the genomics age.

    9th IPSR International Symposium and 5th Symposium on Plant Stress Sciences  2013年 

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  • オオムギ遺伝資源コレクションの全ゲノムSNPフィンガープリンティング.

    日本育種学会講演会  2013年 

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  • タルホコムギの葉と穂のRNA-seq 解析から同定された SNP の6倍体コムギ解析への利用.

    日本育種学会講演会  2013年 

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  • ビールの泡持ちとオオムギ Bホルデイン.

    日本育種学会講演会  2013年 

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  • オオムギの異なる組織に由来するカルスのホルモン分析.

    日本育種学会講演会  2013年 

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  • 「FieldBook」を使ったオオムギ遺伝資源の大規模表現型測定.

    日本育種学会講演会  2013年 

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  • 全ゲノムSNPアレイによるオオムギコアコレクション系統のジェノタイピング

    日本育種学会講演会  2009年 

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  • Proteome analysis of wort, boiled wort, and beer proteins

    The 10th International Barley Genetics Symposium  2008年 

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  • The international barley sequencing consortium (IBSC) - progress towards efficient gene isolation and genomic sequencing in barley

    Plant Genome European Meeting  2008年 

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  • Sequencing Activities On Japanese Malting Barley Haruna Nijo

    Plant & Animal Genome XVI Conference  2008年 

     詳細を見る

  • 並列ゲノムシーケンサーによるオオムギ3H染色体上のBAC配列解析

    日本育種学会講演会  2008年 

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  • 全ゲノムSNPアレイによるオオムギ連鎖地図の作製と遺伝解析

    日本育種学会講演会  2008年 

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  • 簡易型DNAアレイ用いたオオムギSNP検出法システムの試作

    日本育種学会講演会  2008年 

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  • "ビール麦育種における遺伝子多型の応用Ⅰ. 高品質ビール麦品種「はるな二条」と野生オオムギ「H602」の倍加半数体集団で検出された麦芽品質に関するQTL"

    日本育種学会講演会  2008年 

     詳細を見る

  • QTL analysis for malting characteristics based on the high density mapping populations of malting variety, Haruna Nijo, and Hordeum spontaneum, H602.

    The 10th International Barley Genetics Symposium  2008年 

     詳細を見る

  • ビール麦育種における遺伝子多型の応用Ⅱ-Illumina OPA arrayを用いた「ミカモゴールデン」と「Harrington」の倍加半数体集団における麦芽品質のQTL解析-

    日本育種学会講演会  2008年 

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  • 全ゲノムSNPアレイによるオオムギ染色体組換置換系統の高速ジェノタイピング

    日本育種学会講演会  2008年 

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  • オオムギプロテオミックス解析システムの確立

    第二回ムギ類研究会  2007年 

     詳細を見る

  • Detection and identification of haze active proteins through proteome analysis.

    MASTER BREWERS ASSOCIATION OF THE AMERICAS Convention  2007年 

     詳細を見る

  • ビール中タンパク質のプロテオーム解析とその大麦品種間差異

    日本育種学会第112回講演会  2007年 

     詳細を見る

  • 醸造用オオムギはるな二条(Hordeum vulgare cv. Haruna Nijo) の新規な完全長cDNAライブラリーの構築

    日本育種学会第111回講演会  2007年 

     詳細を見る

  • 並列DNA配列解析装置を用いたオオムギゲノム配列解析

    日本育種学会第111回講演会  2007年 

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  • 醸造用オオムギ「はるな二条」(Hordeum vulgare cv. Haruna Nijo)発現遺伝子の網羅的解析を目指した新規完全長cDNAライブラリーの構築

    日本育種学会第111回講演会  2007年 

     詳細を見る

  • DNA マーカーを利用したオオムギ育種の展望

    日本育種学会第49回シンポジウム  2007年 

     詳細を見る

  • 並列DNA配列解析装置によるオオムギBACクローン配列大量解析法の開発

    日本育種学会第112回講演会  2007年 

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  • オオムギのいもち病抵抗性品種遺伝子RMo1はうどんこ病抵抗性遺伝子Mlaを含むR geneクラスター近傍に存在する

    日本育種学会第110回講演会  2006年 

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  • DNAマーカーを利用したオオムギの育種技術

    かずさバイオテクノロジーセミナー2006  2006年 

     詳細を見る

  • オオムギのバイオリソース-多様性とゲノム-

    第47回植物生理学会  2006年 

     詳細を見る

  • QTLs for seed dormancy from wild barley Hordeum vulgare ssp. spontaneum.

    Plant, Animal & Microbe Genomes XIII  2006年 

     詳細を見る

  • Comparisons of Fusarium head blight resistance loci in barley RI populations

    Plant, Animal & Microbe Genomes XIII  2006年 

     詳細を見る

  • オオムギゲノムに基づく育種技術

    CREST終了シンポジウム  2006年 

     詳細を見る

  • オオムギゲノム研究の展開と応用

    日本植物学会大会シンポジウム  2006年 

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  • オオムギの多様性とゲノムリソース

    日本育種学会第110回講演会シンポジウム  2006年 

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  • オオムギ皮裸性遺伝子座を包含するBACコンティグの構築

    日本育種学会第110回講演会  2006年 

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  • ゲノムリソースの開発と応用

    第47回植物生理学会  2006年 

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  • オオムギの多様性を捉えるリソース整備

    第47回植物生理学会  2006年 

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  • オオムギESTを用いた遺伝資源のフィンガープリンティングと連関解析の試み

    日本育種学会第109回講演会  2006年 

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  • オオムギにおける休眠性のQTL解析

    日本育種学会第109回講演会  2006年 

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  • 二条オオムギのトップ交雑に由来するRI集団における赤かび病抵抗性QTLの比較

    日本育種学会第109回講演会  2006年 

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  • オオムギゲノムリソースを利用した休眠性の遺伝解析

    第12回穂発芽研究会  2006年 

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  • Applications of barley EST markers for the analysis of Triticeae germplasm

    ITMI workshop  2006年 

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  • オオムギの種子発芽に関わる遺伝子研究.

    日本植物学会大会  2006年 

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  • 南・東アジアにおけるムギ類多様性へのアプロ-チ.

    日本育種学会  2005年 

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  • オオムギ遺伝資源からのβ-アミラーゼ新規アリルの探索

    日本育種学会  2005年 

     詳細を見る

  • Primary approach for establishing a global proteome map of germinating shoot in malting barley.

    Plant, Animal & Microbe Genomes XIII  2005年 

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  • High density SNP based EST map in barley.

    Plant, Animal & Microbe Genomes XIII  2005年 

     詳細を見る

  • Mapping barley ESTs onto a diploid wheat mapping population.

    Plant, Animal & Mi-crobe Genomes XIII  2005年 

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  • オオムギ組換え自殖集団におけるムギ類萎縮ウイルス抵抗性の評価とQTL解析.

    日本植物病理学会講演会.  2005年 

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  • Introduction of genetic diversity into cereals from their wild relatives.

    5th Int. Triticeae Symp.  2005年 

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  • Comparison of linkage maps based on barley ESTs between diploid wheat and barley.

    SABRAO  2005年 

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  • オオムギ高密度転写産物地図の開発とイネゲノム配列との比較.

    日本育種学会  2005年 

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  • オオムギの網羅的タンパク質マップ構築のためのプロテオーム解析.

    第28回日本分子生物学会年会  2005年 

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  • オオムギ高密度転写産物地図の構築

    日本育種学会  2005年 

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  • オオムギ組換え置換系統群の高解像度ジェノタイピング

    日本育種学会  2005年 

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  • オオムギ休眠性QTLの単離に向けた強連鎖マーカーの検出

    日本育種学会  2005年 

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  • MITE-AFLP法によるオオムギのゲノムマッピング

    日本育種学会  2005年 

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  • オオムギのゲノム研究と種子関連形質の遺伝解析

    日本植物学会  2005年 

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  • オオムギ7H染色体裸性遺伝子座領域のイネとのシンテニー

    日本育種学会第106回講演会  2004年 

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  • Comparative sequence analysis of barley ESTs and rice genome.

    Plant & Animal Genome XII  2004年 

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  • オオムギ倍加半数体および染色体組換置換系統群における農業形質遺伝子座の比較.

    日本育種学会  2004年 

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  • オオムギの異なる交配集団における休眠性QTLの比較.

    日本育種学会  2004年 

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  • 醸造用オオムギ「はるな二条」 BACライブラリーの構築と利用

    日本育種学会  2004年 

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  • オオムギESTマップ作製におけるSNPタイピングシステム

    日本育種学会  2004年 

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  • 二倍体コムギマップ集団へのオオムギESTマーカーの導入.

    日本育種学会  2004年 

     詳細を見る

  • オオムギcDNAマイクロアレイシステムにおけるシグナル強度の統計解析

    日本育種学会  2004年 

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  • 野生オオムギに由来する染色体組換置換系統群の開発

    日本育種学会  2004年 

     詳細を見る

  • オオムギ倍加半数体および染色体組換置換系統群における農業形質遺伝子座の比較

    日本育種学会  2004年 

     詳細を見る

  • オオムギの異なる交配集団における休眠性QTLの比較

    日本育種学会  2004年 

     詳細を見る

  • 醸造用オオムギ「はるな二条」 BACライブラリーの構築と利用

    日本育種学会  2004年 

     詳細を見る

  • オオムギESTマップ作製におけるSNPタイピングシステム

    日本育種学会  2004年 

     詳細を見る

  • 二倍体コムギマップ集団へのオオムギESTマーカーの導入

    日本育種学会  2004年 

     詳細を見る

  • オオムギcDNAマイクロアレイシステムにおけるシグナル強度の統計解析

    日本育種学会  2004年 

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  • QTL Analysis of resistance to fusarium head blight in barley RI populations.

    9th International Barley Genetics Symposium  2004年 

     詳細を見る

  • A large scale mapping of ESTs on barley genome.

    9th International Barley Genetics Symposium  2004年 

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  • O-methyltransferase gene expressed in salt-tolerant barley

    9th International Barley Genetics Symposium  2004年 

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  • Expression analysis using cDNA microarray in seedling shoots of malting barley and its dwarf mutants.

    IPGSA Meeting  2004年 

     詳細を見る

  • Comparison of expression profiles between GeneChip&reg; and cDNA microarray in seedling shoots of malting barley and its dwarf mutants.

    IPGSA Meeting 2004  2004年 

     詳細を見る

  • オオムギESTマーカーを利用したオオムギおよび二倍性コムギにおける第4,第5同祖群の構造推定.

    日本育種学会第106回講演会  2004年 

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  • オオムギの異なるマップ両親におけるESTマーカー多型の比較.

    日本育種学会第106回講演会  2004年 

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  • 異なる系統に由来するオオムギ赤かび病抵抗性QTL.

    日本育種学会第106回講演会  2004年 

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  • オオムギESTデータベース(HarvEST)を利用したPCRマーカーの作成.

    日本育種学会  2003年 

     詳細を見る

  • PCR産物サイズによるオオムギESTのマッピング

    日本育種学会  2003年 

     詳細を見る

  • オオムギESTのイネゲノム上の相同性解析

    日本育種学会  2003年 

     詳細を見る

  • CAPSマーカーによるオオムギESTのマッピング.

    日本育種学会  2003年 

     詳細を見る

  • オオムギ赤かび病抵抗性の発現と選抜

    日本育種学会  2003年 

     詳細を見る

  • オオムギcDNAマイクロアレイの試作.

    日本育種学会  2003年 

     詳細を見る

  • オオムギESTに由来するPCR産物のマップ親における多型.

    日本育種学会  2003年 

     詳細を見る

  • 二条オオムギ間の交雑に由来するRI集団におけるオオムギ赤カビ病抵抗性のQTL解析

    日本育種学会  2003年 

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  • オオムギにおけるアルミニウム耐性機構の遺伝学的解析

    2003年度日本土壌肥料学会講演要旨集 p.68  2003年 

     詳細を見る

  • A new secretion pattern of citrate is triggered by Al in barley

    日本植物生理学会  2003年 

     詳細を見る

  • オオムギESTマーカーの大規模開発

    日本育種学会  2003年 

     詳細を見る

  • オオムギcDNAライブラリを用いた遺伝子発現プロファイルの大規模解析のためのデータベース構築

    日本分子生物学会年会  2002年 

     詳細を見る

  • SNP detection from barley EST resources in Okayama Univertisy

    Plant and Animal Genome X  2002年 

     詳細を見る

  • Construction of a BAC library from Japanese malting barley Haruna Nijo

    Plant and Animal Genome X  2002年 

     詳細を見る

  • 塩ストレス抵抗性オオムギに関する研究.1.塩ストレスで特異的に発現する遺伝子の解析

    日本育種学会  2002年 

     詳細を見る

  • 高能率ゲノム走査法(HEGS)によるオオムギ高密度連鎖地図の短期作成

    日本育種学会  2002年 

     詳細を見る

  • ダイレクトシーケンシングによるオオムギESTに由来するSNPの再確認

    日本育種学会  2002年 

     詳細を見る

  • オオムギにおけるアルミニウム耐性遺伝資源の選抜

    日本育種学会  2002年 

     詳細を見る

  • オオムギ赤かび病抵抗性のQTL解析

    日本育種学会  2002年 

     詳細を見る

  • オオムギESTからのUnigene構築法の比較

    日本育種学会  2002年 

     詳細を見る

  • 中国およびその周辺国から導入したオオムギ遺伝資源の多様性評価とコアサンプルの選抜

    日本育種学会  2002年 

     詳細を見る

  • オオムギのESTに由来するSTS配列の多型性

    日本育種学会第102回講演会  2002年 

     詳細を見る

  • オオムギESTのphred/phrap大規模解析ならびにライブラリ特異的配列の探索.

    日本育種学会第102回講演会  2002年 

     詳細を見る

  • 高密度連鎖地図を用いたオオムギ赤カビ病抵抗性のQTL解析

    日本育種学会第102回講演会  2002年 

     詳細を見る

  • 塩ストレス抵抗性オオムギに関する研究.2.塩ストレスで誘導されるO-methyltransferase遺伝子のクローニングと構造

    日本育種学会第102回講演会  2002年 

     詳細を見る

  • オオムギESTsに存在するSNPsの特性

    日本分子生物学会年会  2002年 

     詳細を見る

  • マイクロサテライトマーカーを用いたオオムギ遺伝資源の多様性評価

    日本育種学会  2001年 

     詳細を見る

  • オオムギcDNAクローンの大量シーケンシング

    日本育種学会  2001年 

     詳細を見る

  • 中国辺境および周辺国に分布するオオムギ遺伝資源の導入と特性評価

    日本育種学会  2001年 

     詳細を見る

  • バルクセグレガント分析を用いたオオムギアルミニウム耐性のマッピング

    日本育種学会  2001年 

     詳細を見る

  • 中国中南部から収集したオオムギ遺伝資源のマイクロサテライトマーカーによる評価

    日本育種学会  2001年 

     詳細を見る

  • A Genomic Region Associated with Aluminium Tolerance in Barley.

    Australasian Barley Technical Symposium  2001年 

     詳細を見る

  • The International barley core collection

    4th International Triticeae Symposium, Cordoba, Spain  2001年 

     詳細を見る

  • Genetic diversity in barley (Hordeum vulgare)

    4th International Triticeae Symposium  2001年 

     詳細を見る

  • オオムギESTに見いだされた一塩基置換多型

    日本育種学会  2001年 

     詳細を見る

  • オオムギアルミニウム耐性の精密マッピングに向けたマップ集団の再構成

    日本育種学会  2001年 

     詳細を見る

  • RI集団を用いたオオムギ赤かび病抵抗性の連鎖分析

    日本育種学会  2001年 

     詳細を見る

  • QTL analysis for agronomic traits in a barley cross.

    8th Barley Genetics Symposium  2000年 

     詳細を見る

  • オオムギのアルミニウム耐性に関するバルクセグレガント分析

    日本育種学会  2000年 

     詳細を見る

  • オオムギにおける酸性土壌耐性の大量検定法

    日本育種学会  2000年 

     詳細を見る

  • オオムギ成植物の葉に由来するcDNAライブラリーのシーケンス特性

    日本育種学会  2000年 

     詳細を見る

  • 南・東アジアのオオムギコアコレクションとそのマイクロサテライト多型

    日本育種学会  2000年 

     詳細を見る

  • オオムギのcDNAシークエンス解析 : ビールオオムギ「はるな二条」と日本在来品種「赤神力」との比較

    日本育種学会  2000年 

     詳細を見る

  • マイクロサテライトマーカーを用いたオオムギフィンガープリンティング法の開発

    日本育種学会  2000年 

     詳細を見る

  • Exploiting Hordeum vulgare ssp spontaneum genetic resources: diversity analysis and germ-plasm development

    8th Barley Genetics Symposium  2000年 

     詳細を見る

  • Preliminary Sequencing of Clones in a cDNA Library from Barley Adult Leaves

    8th Barley Genetics Symposium  2000年 

     詳細を見る

  • QTL Analysis for Barley Yellow Mosaic Virus Resistance in a Chinese Barley Landrace, Mokusekko 3.

    8th Barley Genetics Symposium  2000年 

     詳細を見る

  • Analysis of Simple Sequence Repeat Length Polymorphism (SSLP) in Barley

    8th Barley Genetics Symposium  2000年 

     詳細を見る

  • QTL analysis of malting quality in the Harrington x Morex cross.

    8th Barley Genetics Symposium  2000年 

     詳細を見る

  • オオムギにおける酸性土壌耐性評価法の確立

    日本育種学会講演会  1999年 

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  • オオムギの麦芽エキスと穀皮歩合に関するQTLの関係

    日本育種学会講演会  1999年 

     詳細を見る

▼全件表示

Works(作品等)

  • Release of new malting barley variety ''Ryohfu''

     詳細を見る

  • Release of new feed barley variety ''Aominori''

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  • Release of new malting barley variety ''Ryohfu''

     詳細を見る

  • Release of new feed barley variety ''Aominori''

     詳細を見る

  • 二条大麦品種「りょうふう」の育成

     詳細を見る

  • 飼料用大麦品種「あおみのり」の育成

     詳細を見る

▼全件表示

受賞

  • 山陽新聞賞

    2020年1月  

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  • 日本醸造協会技術賞

    2017年  

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    受賞国:日本国

    researchmap

  • 村川技術奨励賞

    2016年  

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    受賞国:日本国

    researchmap

  • 日本育種学会賞

    2013年  

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    受賞国:日本国

    researchmap

  • 北農賞

    1992年  

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    受賞国:日本国

    researchmap

 

担当授業科目

  • 植物ゲノム多様性解析学 (2020年度) 後期  - その他

  • 植物多様性解析学演習 (2020年度) 前期  - その他

  • 植物多様性解析学演習 (2020年度) 後期  - その他

  • 植物多様性解析学演習 (2020年度) 後期  - その他

  • 植物多様性解析学演習 (2020年度) 前期  - その他

  • 植物多様性遺伝学 (2020年度) 前期  - その他

  • 生物資源科学特別研究 (2020年度) 通年  - その他

  • 生物資源科学特論II (2020年度) 夏季集中  - その他

  • Advances in Plant Stress Science (2020年度) 後期  - その他

▼全件表示