2024/11/07 更新

写真a

タサキ　ヒデタカ
田﨑　秀尚
TASAKI Hidetaka
所属
生殖補助医療技術教育研究センター 助教
職名
助教
ホームページ
https://sites.google.com/s.okayama-u.ac.jp/armunit
外部リンク

学位

  • 博士（畜産学） （ 東京農業大学 ）

研究キーワード

  • 繁殖生物学

  • 卵巣

  • 生殖細胞

  • 生殖医療

  • 卵母細胞

  • 老化

  • 妊孕性温存

研究分野

  • ライフサイエンス / 動物生命科学

  • ライフサイエンス / 産婦人科学

  • ライフサイエンス / 動物生産科学

  • ライフサイエンス / 細胞生物学

経歴

  • 岡山大学   生殖補助医療技術教育研究センター   助教

    2019年7月 - 現在

    　 詳細を見る

  • 岡山大学   学術研究院 環境生命自然科学学域   助教（兼担）

    2023年4月 - 現在

    　 詳細を見る

  • 岡山大学   学術研究院 環境生命科学学域   助教（兼担）

    2021年4月 - 2023年3月

    　 詳細を見る

  • 岡山大学   大学院 環境生命科学研究科   助教（兼担）

    2019年7月 - 2021年3月

    　 詳細を見る

所属学協会

  • 生殖補助医療技術教育カリキュラム標準化懇談会

    2019年 - 現在

    　 詳細を見る

  • 日本受精着床学会

    2017年 - 現在

    　 詳細を見る

  • 日本生殖医学会

    2016年 - 現在

    　 詳細を見る

  • 日本卵子学会

    2015年 - 現在

    　 詳細を見る

  • 日本繁殖生物学会

    2011年 - 現在

    　 詳細を見る

 

論文

  • Effect of low ethanol concentration in maturation medium on developmental ability, mitochondria, and gene expression profile in mouse oocytes. 査読 国際誌

    Susaki Kizasu, Takuya Sato, Yuki Inoue, Hidetaka Tasaki, Komei Shirasuna, Yuichi Okiishi, Hisataka Iwata

    Reproductive biology   24 ( 2 )   100854 - 100854   2024年6月

    　詳細を見る

    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    Ethanol affects pre-conceptional oocyte quality in women. In this study, we examined the effect of low ethanol concentrations on mouse oocytes. Oocytes were collected from the ovaries of 9-10 week old mice and allowed to mature in vitro in the presence of low concentrations of ethanol (0.1% and 0.2% v/v) for 24 h. Treatment of oocytes with ethanol (0.2%) during maturation decreased the mitochondrial DNA content and membrane potential compared to that in untreated ones, whereas the ATP content did not differ between the groups. Both 0.1% and 0.2% ethanol reduced the lipid content in the oocytes. In addition, immunostaining revealed that oocytes cultured in maturation medium containing ethanol (0.2%) had reduced levels of global DNA methylation and DNMT3A compared with untreated oocytes, and decreased rate of blastocyst development with low mitochondrial protein levels (TOMM40) in embryo. RNA-sequencing of the ethanol-treated (0.2%) and untreated oocytes revealed that mitochondria were a major target of ethanol. In conclusion, treatment of oocytes with low concentration of ethanol reduces the developmental rate to the blastocyst stage, with a lower total cell number and global DNA methylation. In addition, ethanol affected mitochondrial function and mitochondria-related gene expression.

    DOI： 10.1016/j.repbio.2023.100854

    PubMed

    researchmap

  • The effect of betaine for mouse sperm cryopreservation 査読 国際誌

    Natsuho Mori, Moeka Ishihara, Hidetaka Tasaki, Tadashi Sankai, Junko Otsuki

    Cryobiology   106   157 - 159   2022年4月

    　詳細を見る

    記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Elsevier {BV}  

    Sperm cryopreservation is an effective method of preserving male fertility in humans, as well as domestic and experimental animals. However, various factors such as ice crystal formation, osmotic stress, and oxidative stress, negatively influence the motility and viability of post-thawed spermatozoa. Betaine, which works as an osmoprotectant is known to work as a nontoxic cryoprotectant. However, the protective effects during mouse sperm cryopreservation are still unclear. Thus, the purpose of this study was to investigate whether betaine has protective effects during the process of mouse sperm cryopreservation. In this study, betaine was found to be effective in maintaining sperm motility during the freezing procedure and 1% (85.4 mM) betaine was identified as the optimal concentration to be added to cryopreservation solutions. It was also found that betaine improves the integrity of the plasma membranes of sperm tails, suggesting that betaine has a positive effect on sperm motility.

    DOI： 10.1016/j.cryobiol.2022.03.006

    Scopus

    PubMed

    researchmap

  • Effect of docosahexaenoic acid on in vitro growth of bovine oocytes 査読

    Shuta Nagata, Kaoru Tatematsu, Hitoki Yamaguchi, Yuki Inoue, Keisuke Tanaka, Hidetaka Tasaki, Koumei Shirasuna, Hisataka Iwata

    Reproductive Medicine and Biology   20 ( 4 )   485 - 493   2021年10月

    　詳細を見る

    記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Wiley  

    PURPOSE: The present study investigated the effects of docosahexaenoic acid (DHA) on the growth of bovine oocytes. METHODS: Oocytes and granulosa cell complexes (OGCs) were collected from early antral follicles (0.4-0.7 mm) on the surface of ovaries harvested from a slaughterhouse. The OGCs were cultured with 0, 1, and 10 μmol/L docosahexanoic acid (DHA) for 16 days. RESULTS: Antrum formation of the OGCs and the number of granulosa cells (GCs) surrounding the oocytes were comparable among groups, whereas supplementation of 0.1 μmol/L of DHA significantly improved oocyte growth. Oocytes grown with DHA had a higher fertilization rate, acetylation levels of H4K12, and ATP contents, as well as a lower lipid content compared with those grown without DHA. In addition, GCs surrounding OGCs grown with DHA had low lipid content compared with vehicle counterparts. Furthermore, when GCs were cultured in vitro, DHA increased ATP production, mitochondrial membrane potential, and reduced lipid content and levels of reactive oxygen species. RNA-seq of GCs revealed that DHA increased CPT1A expression levels and affect genes associated with focal adhesion, oxidative phosphorylation, and PI3K-AKT etc. CONCLUSION: The results suggest that DHA supplementation affects granulosa cell characteristics and supports oocyte growth in vitro.

    DOI： 10.1002/rmb2.12403

    Scopus

    PubMed

    researchmap

    その他リンク： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/rmb2.12403

  • Nuclear-to-cytoplasmic ratios of 1PN and 2PN zygotes after in vitro fertilization of mouse oocytes 査読 国際共著 国際誌

    Natsumi Okajima, Wei Xiao, Alex Lopata, Tadashi Sankai, Lubna Yasmin, Yasushi Nagai, Ryota Okamoto, Hidetaka Tasaki, Junko Otsuki

    Zygote   30 ( 1 )   1 - 5   2021年6月

    　詳細を見る

    記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Cambridge University Press (CUP)  

    <title>Summary</title>
    Numerous studies have reported comparisons of the nuclear-to-cytoplasmic (NC) ratio during mitosis. However, little information is known about how the pronuclear size is regulated and determined at the end of meiosis II in mammalian zygotes. The present study aims to analyze the NC ratio of female and male pronuclei, and also to compare the size of single pronuclei using photographs that were obtained during experiments to create chimeric hermaphrodites from 2-cell oocytes. The volume of both the female and the male pronucleus was found to correlate with the volume of the oocyte cytoplasm. The NC ratio of the male pronucleus was greater than that of the female pronucleus. The NC ratio of the average volume of the female and male pronuclei was greater than the NC ratio of the mononucleate oocytes. The occurrence of 1PN oocytes was significantly higher when the volume of cytoplasm was lower than the cut-off value. These results indicated that the NC ratio is retained during pronuclear formation. A higher NC ratio in male compared with the female pronucleus indicated structural and/or molecular difference between the two pronuclei. 1PN formation may occur when sperm enters close to the MII spindle.

    DOI： 10.1017/s096719942100040x

    Scopus

    PubMed

    researchmap

  • Upregulation of CHOP participates in caspase activation and virus release in human astrovirus-infected cells 査読 国際誌

    Tomoyasu Isobe, Shoichiro Tange, Hidetaka Tasaki, Kumiko Kanamori, Akiko Kato, Akira Nakanishi

    Journal of General Virology   100 ( 5 )   778 - 792   2019年5月

    　詳細を見る

    記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Microbiology Society  

    Human astroviruses (HAstVs), non-enveloped RNA viruses with positive-sense RNA genomes, are an important cause of acute gastroenteritis in young children, although the processes that produce infectious virions are not clearly defined. To track the viral replication complex (RC) upon HAstV1 infection, the subcellular distribution of double-stranded (ds) RNA and of ORF1b, a viral RNA polymerase, was examined by immunocytochemistry. Foci that were positive for dsRNA and for ORF1b were co-localized, and both foci were also co-localized with resident proteins of the endoplasmic reticulum (ER). Focusing on the association between the HAstV RC and ER, we examined the expression of unfolded protein response (UPR) markers and found that targets of eukaryotic translation initiation factor 2α (eIF2α)-activating transcription factor 4 (ATF4), including CCAAT/enhancer-binding protein homologous protein (CHOP), a proapoptotic transcription factor, were upregulated at the late phase in HAstV-infected cells. Consistently, eIF2α was phosphorylated at the late phase of HAstV infection. The formation of foci resembling stress granules, another known downstream response to eIF2α phosphorylation, was also observed at the same period. Phosphorylation of eIF2α was attenuated in protein kinase R (PKR)-knockdown cells, suggesting that, unlike the canonical ER stress response, PKR was involved in eIF2α phosphorylation in response to HAstV infection. Studies have indicated that immature HAstV capsid protein is processed by caspases, and caspase cleavage is integral to particle release. Inhibition of CHOP upregulation reduced caspase activation and the release of HAstV RNA from cells during HAstV infection. Our results suggest that the eIF2α-ATF4-CHOP pathway participates in HAstV propagation.

    DOI： 10.1099/jgv.0.001250

    Scopus

    PubMed

    researchmap

  • Relationship between the number of cells surrounding oocytes and energy states of oocytes 査読 国際誌

    Yasuhisa Munakata, Tomoya Ichinose, Kaori Ogawa, Nobuhiko Itami, Hidetaka Tasaki, Koumei Shirasuna, Takehito Kuwayama, Hisataka Iwata

    THERIOGENOLOGY   86 ( 7 )   1789 - 1798   2016年10月

    　詳細を見る

    記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ELSEVIER SCIENCE INC  

    Lipid content, ATP content, and histone acetylation are thought to reflect the energy state of cells. In addition, the energy state closely associates with growth and developmental ability of oocytes. Oocyte growth is accompanied by active proliferation of the surrounding granulosa cells (GCs), and GCs play a key role in the provision of energy substrates to the oocytes. In the present study, we first examined the relationship among the average number of GCs per follicle, the average number of cumulus cells (CCs) per oocyte, and the average lipid content in oocytes that developed in vivo within individual donor gilts. Second, we validated the relationship between the number of cells surrounding oocytes and the energy states of oocytes by using an IVC system of oocyte granulosa cell complexes (OGCs) derived from early antral follicles. We collected cumulus cells and oocyte complexes (COCs) from antral follicles (3-5 mm in diameter) and found that average lipid content in oocytes significantly correlated with the average number of both GCs/follicle and CCs/oocyte (P &lt; 0.05). In the next series of experiments, we collected OGCs from early antral follicles (0.5-0.7 mm in diameter), and cultured them for 14 days, and then determined the cell number of OGCs, as well as the lipid content, ATP content, and acetylation level of H4K12 in oocytes grown in vitro. In addition, glucose consumption by OGCs was calculated from the sample media collected at Days 13 and 14. The lipid content of oocytes grown in vitro, significantly correlated with the number of cells surrounding the oocytes (P &lt; 0.01) and with the level of glucose consumption by OGCs (P &lt; 0.01). In addition, both ATP content and H4K12 acetylation levels of oocytes grown in vitro significantly correlated with the number of cells surrounding the oocytes (P &lt; 0.05) and glucose consumption by OGCs (P &lt; 0.05). In conclusion, the lipid content of oocytes depends on the number of cells surrounding the oocytes, and glucose uptake by OGCs is crucial for lipid content and ATP content, and H4K12 acetylation in oocytes. (C) 2016 Elsevier Inc. All rights reserved.

    DOI： 10.1016/j.theriogenology.2016.05.036

    Web of Science

    Scopus

    PubMed

    researchmap

  • Gene expression patterns in granulosa cells and oocytes at various stages of follicle development as well as in in vitro grown oocyte-and-granulosa cell complexes 査読

    Yasuhisa Munakata, Ryoka Kawahara-Miki, Shogo Shiratsuki, Hidetaka Tasaki, Nobuhiko Itami, Koumei Shirasuna, Takehito Kuwayama, Hisataka Iwata

    JOURNAL OF REPRODUCTION AND DEVELOPMENT   62 ( 4 )   359 - 366   2016年8月

    　詳細を見る

    記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SOCIETY REPRODUCTION & DEVELOPMENT-SRD  

    Follicle development is accompanied by proliferation of granulosa cells and increasing oocyte size. To obtain high-quality oocytes in vitro, it is important to understand the processes that occur in oocytes and granulosa cells during follicle development and the differences between in vivo and in vitro follicle development. In the present study, oocytes and granulosa cells were collected from early antral follicles (EAFs, 0.5-0.7 mm in diameter), small antral follicles (SAFs, 1-3 mn in diameter), large antral follicles (LAFs, 3-7 mm in diameter), and in vitro grown oocyte-and-granulosa cell complexes (OGCs), which were cultured for 14 days after collection from EAFs. Gene expression was analyzed comprehensively using the next-generation sequencing technology. We found top upstream regulators during the in vivo follicle development and compared them with those in in vitro developed OGCs. The comparison revealed that HIFI is among the top regulators during both in vivo and in vitro development of OGCs. In addition, we found that HIF1-mediated upregulation of glycolysis in granulosa cells is important for the growth of OGCs, but the cellular metabolism differs between in vitro and in vivo grown OGCs. Furthermore, on the basis of comparison of upstream regulators between in vivo and in vitro development of OGCs, we believe that low expression levels of FLT1 (VEGFA receptor), SPP1, and PCSK6 can be considered causal factors of the suboptimal development under in vitro culture conditions.

    DOI： 10.1262/jrd.2016-022

    Web of Science

    Scopus

    PubMed

    researchmap

  • Examination of a plasmid-based reverse genetics system for human astrovirus 査読 国際誌

    Benoit Chapellier, Shoichiro Tange, Hidetaka Tasaki, Kazuhiro Yoshida, Yan Zhou, Naomi Sakon, Kazuhiko Katayama, Akira Nakanishi

    MICROBIOLOGY AND IMMUNOLOGY   59 ( 10 )   586 - 596   2015年10月

    　詳細を見る

    記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：WILEY-BLACKWELL  

    A plasmid-based reverse genetics system for human astrovirus type 1 (HAstV1) is examined. Upon transfection into 293T cells, the plasmid vector, which harbors a HAstV1 expression cassette, expressed astroviral RNA that appeared to be capable of viral RNA replication, as indicated by the production of subgenomic RNA and capsid protein expression irrespective of the heterologous 5 ends of the transcribed RNA. Particles infectious to Caco-2 cells were made in this system; however, their infectivity was much lower than would be expected from the amount of particles apparently produced. Using Huh-7 cells as the transfection host with the aim of improving viral capsid processing for virion maturation partially restored the efficiency of infectious particle formation. Our results support the possibility that the DNA transfection process induces a cellular response that targets late, but not early, stages of HAstV1 infection.

    DOI： 10.1111/1348-0421.12317

    Web of Science

    Scopus

    PubMed

    researchmap

  • Effects of reaggregated granulosa cells and oocytes derived from early antral follicles on the properties of oocytes grown in vitro 査読

    Ayano Oi, Hidetaka Tasaki, Yasuhisa Munakata, Koumei Shirasuna, Takehito Kuwayama, Hisataka Iwata

    JOURNAL OF REPRODUCTION AND DEVELOPMENT   61 ( 3 )   191 - 197   2015年6月

    　詳細を見る

    記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SOCIETY REPRODUCTION & DEVELOPMENT-SRD  

    In this study, we examined the effects of reconstructed oocyte granulosa cell complexes (OGCs) on the development of porcine oocytes derived from early antral follicles (EAFs; 0.5-0.7 mm in diameter). When denuded oocytes were cocultured with granulosa cells derived from other EAFs, the oocytes and granulosa cells aggregated to form OGCs after 2 days of culture. After 14 days of culture, we compared cell number, oocyte diameter, and oocyte chromatin configuration in unmanipulated (natural) OGCs, reconstructed OGCs, and OGCs collected from antral follicles (AFs, 3.0-6.0 mm in diameter). The diameters of oocytes from reconstructed OGCs grown in vitro were not different from those of oocytes from natural OGCs, although they were significantly smaller than those of oocytes from antral follicle (AF) OGCs. Oocyte chromatin configuration did not differ among the 3 OGC groups, but the oocyte nuclear maturation rate was lower in the reconstructed OGCs and higher in the AF OGCs. However, when the in vitro culture period for the reconstructed OGCs was extended by 2 days, the nuclear maturation rate of oocytes from reconstructed OGCs was similar to that of oocytes from natural OGCs. In addition, blastocysts were successfully obtained from oocytes from reconstructed OGCs. In conclusion, we established an innovative culture method that allows oocytes and granulosa cells from EAFs to reaggregate as reconstructed OGCs, which yield oocytes with the ability to develop to the blastocyst stage.

    DOI： 10.1262/jrd.2014-123

    Web of Science

    Scopus

    PubMed

    researchmap

  • The Effect of High Glucose Concentration on the Quality of Oocytes Derived from Different Growth Stages of Follicles 査読

    Tasaki H, Munakata Y, Arai S, Murakami S, Kuwayama T, Iwata H

    Journal of Mammalian Ova Research   32 ( 1 )   41 - 48   2015年

    　詳細を見る

    記述言語：英語   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1274/jmor.32.41

    Scopus

    researchmap

  • Relationship between mitochondrial DNA Copy Number and SIRT1 Expression in Porcine Oocytes 査読 国際誌

    Daichi Sato, Nobuhiko Itami, Hidetaka Tasaki, Shun Takeo, Takehito Kuwayama, Hisataka Iwata

    PLOS ONE   9 ( 4 )   e94488   2014年4月

    　詳細を見る

    記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：PUBLIC LIBRARY SCIENCE  

    The present study assessed the effect of resveratrol on the expression of SIRT1 and mitochondrial quality and quantity in porcine oocytes. Supplementing the maturation medium with 20 mu M resveratrol increased the expression of SIRT1, and enhanced mitochondrial functions, as observed from the increased ATP content and mitochondrial membrane potential. Addition of resveratrol also improved the ability of oocytes to develop into the blastocyst stage following activation. The effects of resveratrol on mitochondrial number were examined by comparing the mitochondrial DNA copy number (Mt number) between group of oocytes collected from the same donor gilt ovaries. Supplementing the maturation medium with only resveratrol did not affect the Mt number in the oocytes. However, supplementing the maturation medium with 10 mu M MG132, a proteasome inhibitor, significantly increased the amount of ubiquitinated proteins and Mt number by 12 and 14%, respectively. In addition, when resveratrol was added to the medium containing MG132, the Mt number increased significantly by 39%, this effect was diminished by the addition of the SIRT1 inhibitor EX527. Furthermore, supplementing the medium with MG132 and EX527 did not affect Mt number. The mean SIRT1 expression in 20 oocytes was significantly and positively correlated with the Mt number in oocytes collected from the same donor. This study suggests that the expression of SIRT1 is associated with the Mt number in oocytes. In addition, activation of SIRT1 by resveratrol enhances the biosynthesis and degradation of mitochondria in oocytes, thereby replenishing and improving mitochondrial function and the developmental ability of oocytes.

    DOI： 10.1371/journal.pone.0094488

    Web of Science

    Scopus

    PubMed

    researchmap

  • Estradiol has a major role in antrum formation of porcine preantral follicles cultured in vitro 査読 国際誌

    Hidetaka Tasaki, Hisataka Iwata, Daichi Sato, Yasunori Monji, Takehito Kuwayama

    THERIOGENOLOGY   79 ( 5 )   809 - 814   2013年3月

    　詳細を見る

    記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ELSEVIER SCIENCE INC  

    Antrum formation and estradiol (E2) secretion occur during early folliculogenesis. The objective was to determine the role of E2 in antrum formation of oocyte-granulosa cell complexes (OGCs) derived from porcine preantral follicles (PAFs). Supplementation of the culture medium with E2 (1 mu g/mL) improved antrum formation of OGCs during 14 days of in vitro culture. Furthermore, adding 0.1 mu g/mL androstenedione (a precursor of E2) to the medium also improved antrum formation. Concentration of E2 was higher in the medium of developmentally competent OGCs versus incompetent OGCs (8.5 vs. 3.5 ng/mL, P &lt; 0.05). Fulvestrant (1 mu g/mL), a competitive inhibitor of E2, completely inhibited antrum formation of OGCs that were cultured in medium containing either E2 (0.1 mu g/mL) or androstenedione (0.1 mu g/mL); however, increasing E2 to 1 mu g/mL ameliorated the inhibitory effect. Conversely, in the case of early antral follicles, OGCs formed antrums without E2 supplementation. After E2 pretreatment, OGCs derived from PAFs formed antrums even when the OGCs were subsequently cultured in medium without E2. Furthermore, when OGCs derived from PAFs were cultured without E2 followed by an additional in vitro culture with E2, antrums were formed, albeit with the same period delay by the same pretreatment periods. In conclusion, E2 in the culture medium was indispensable for in vitro antrum formation of OGCs derived from PAFs; therefore, one of the roles of E2 is in the initiation of antrum formation. (c) 2013 Elsevier Inc. All rights reserved.

    DOI： 10.1016/j.theriogenology.2012.12.009

    Web of Science

    Scopus

    PubMed

    researchmap

▼全件表示

MISC

  • 体外発育培養が老化マウス由来未発育卵胞のトランスクリプトームならびに染色体分配に及ぼす影響

    田崎 秀尚, 小松 千尋, 園 菜々美, 隈本 宗一郎, 井上 裕貴, 大月 純子, 岩田 尚孝

    Journal of Mammalian Ova Research   40 ( 1 )   S34 - S34   2023年4月

    　詳細を見る

    記述言語：日本語   出版者・発行元：(一社)日本卵子学会  

    researchmap

  • 顕微授精技術を用いた無精子症マウスからの産子作出

    越後貫成美, 日野敏昭, 大澤優生, 藤原靖浩, 水野聖哉, 井上貴美子, 井上貴美子, 国枝哲夫, 田崎秀尚, 大月純子, 立野裕幸, 杉山文博, 小倉淳郎, 小倉淳郎, 小倉淳郎

    日本実験動物学会総会講演要旨集(Web)   70th   2023年

    　詳細を見る

講演・口頭発表等

  • In Vitro Culture Improves the Transcriptome and Chromosome Structure of Secondary Follicles in Aging Mice.

    Hidetaka Tasaki, Chihiro Komatsu, Nanami Sono, Soichiro Kumamoto, Yuki Inoue, Hisataka Iwata

    Society for the Study of Reproduction 57th Annual Meeting  2024年7月17日 

    　詳細を見る

    開催年月日： 2024年7月16日 - 2024年7月19日

    会議種別：ポスター発表  

    researchmap

  • 体外発育培養が老化マウス由来未発育卵胞に及ぼす影響

    田﨑 秀尚

    第4回有性生殖研究会  2024年3月8日 

    　詳細を見る

    開催年月日： 2024年3月8日 - 2024年3月9日

    会議種別：ポスター発表  

    researchmap

  • 体外発育培養が老化マウス由来未発育卵胞のトランスクリプトームならびに染色体分配異常に及ぼす影響

    田﨑秀尚, 小松千尋, 園菜々美, 隈本宗一郎, 井上裕貴, 大月純子, 岩田尚孝

    第64回日本卵子学会学術集会  2023年5月20日 

    　詳細を見る

    開催年月日： 2023年5月20日 - 2023年5月21日

    researchmap

  • マウス卵細胞膜の修復機構におけるカルシウムイオンの関与

    田崎 秀尚, 山中 寛子, 大月 純子

    第40回受精着床学会総会・学術講演会  2022年7月28日 

    　詳細を見る

    開催年月日： 2022年7月28日 - 2022年7月29日

    会議種別：口頭発表（一般）  

    researchmap

  • 浸透圧ストレスに対する卵細胞膜の修復機構

    田﨑 秀尚, 山中 寛子, 大月 純子

    第63回日本卵子学会学術集会  2022年5月23日 

    　詳細を見る

    開催年月日： 2022年5月24日

    会議種別：口頭発表（一般）  

    researchmap

  • 受精過程におけるHaloの継続時間と胚発生および妊娠成績との相関に関する検討

    田崎秀尚, 江副賢二, 三木哲也, 沖村匡史, 内山一男, 薮内晶子, 張士青, 中川優子, 藤田裕, 土山哲史, 篠原一朝, 小林保, 加藤恵一

    第37回日本受精着床学会学術講演会  2019年8月 

    　詳細を見る

  • 体外成熟培地へのアラニン、グルタミン酸、グリシンの添加がウシ未成熟卵子の核成熟および胚発生能に及ぼす影響.

    田崎秀尚, 江副賢二, 薮内晶子, 中川優子, 安藤郷子, 山崎裕行, 小林保, 加藤恵一

    第63回日本生殖医学会学術講演会  2018年9月 

    　詳細を見る

    記述言語：日本語   会議種別：口頭発表（一般）  

    researchmap

  • 体外成熟培地への必須および非必須アミノ酸添加がウシ卵子の核成熟ならびに胚発生能に及ぼす影響

    田崎秀尚, 江副賢二, 薮内晶子, 濱田雄行, 洲河美貴, 佐藤團, 和田恵子, 小林保, 加藤恵一

    第36回日本受精着床学会学術講演会  2018年7月 

    　詳細を見る

    記述言語：日本語   会議種別：口頭発表（一般）  

    researchmap

  • Effect of essential and non-essential amino acids in in vitro maturation medium on the maturation and embryo development of bovine follicular oocytes.

    Tasaki H, Ezoe K, Yabuuchi A, Kobayashi T, Kato K

    The 8th Congress of the Asia Pacific Initiative on Reproduction  2018年4月 

    　詳細を見る

    記述言語：英語   会議種別：口頭発表（一般）  

    researchmap

  • Cryotop法におけるガラス化保存がマウス卵子のF-actin分布に及ぼす影響.

    田崎秀尚, 江副賢二, 薮内晶子, 小林保, 加藤恵一

    第35回日本受精着床学会学術講演会  2017年7月 

    　詳細を見る

    記述言語：日本語   会議種別：口頭発表（一般）  

    researchmap

  • Cryotop法におけるガラス化保存がマウス卵子のアクチンキャップに及ぼす影響.

    田崎秀尚, 江副賢二, 薮内晶子, 小林保, 加藤恵一

    日本A-PART学術講演会2017  2017年3月 

    　詳細を見る

    記述言語：日本語   会議種別：ポスター発表  

    researchmap

  • Cryotop法の平衡過程における凍結保護物質の濃度変化がマウス卵子の胚発生に及ぼす影響.

    田崎秀尚, 大畠一輝, 森智絵美, 江副賢二, 薮内晶子, 奥野隆, 小林保, 加藤恵一

    第61回日本生殖医学会学術講演会  2016年11月 

    　詳細を見る

    記述言語：日本語   会議種別：口頭発表（一般）  

    researchmap

  • Acquisition of meiotic competence in porcine secondary follicles following long-term culture.

    Tasaki H, Itami N, Iwata H

    The 3rd World Congress on Reproductive Biology  2014年9月 

    　詳細を見る

    記述言語：英語   会議種別：ポスター発表  

    researchmap

  • 卵胞腔形成前後のブタ発育途上卵子顆粒層細胞複合体の体外発育について.

    田崎秀尚, 大井綾野, 伊丹暢彦, 桑山岳人, 岩田尚孝

    第107回日本繁殖生物学会大会  2014年8月 

    　詳細を見る

    記述言語：日本語   会議種別：ポスター発表  

    researchmap

  • 卵子のSIRT1活性化はミトコンドリアの更新を介して卵子の質を向上する.

    田崎秀尚, 佐藤大地, 小笠駿, 田村美咲, 東本隼人, 原梨沙子, 藤岡寿文, 谷津恵, 門司恭典, 桑山岳人, 岩田尚孝

    第118回日本畜産学会  2014年3月 

    　詳細を見る

    記述言語：日本語   会議種別：口頭発表（一般）  

    researchmap

  • ブタ卵胞の腔形成は17β-Estradiolによるギャップ結合の調節により誘導される.

    田崎秀尚, 大井綾野, 門司恭典, 桑山岳人, 岩田尚孝

    第36回日本分子生物学会年会  2013年12月 

    　詳細を見る

    記述言語：日本語   会議種別：ポスター発表  

    researchmap

  • 高グルコース濃度がブタ初期胞状卵胞由来卵子の体外発育能力に及ぼす影響.

    田崎秀尚, 新井彩貴, 西村萌, 村上覚史, 門司恭典, 桑山岳人, 岩田尚孝

    第106回日本繁殖生物学会大会  2013年9月 

    　詳細を見る

    記述言語：日本語   会議種別：ポスター発表  

    researchmap

  • 17β-estradiol がブタ前胞状卵胞由来卵子の腔形成に与える影響.

    田崎秀尚, 大井綾野, 岩田尚孝, 門司恭典, 桑山岳人

    第105回日本繁殖生物学会大会  2012年9月 

    　詳細を見る

    記述言語：日本語   会議種別：ポスター発表  

    researchmap

  • Effect of estradiol on in vitro development of porcine preantral follicles.

    Tasaki H, Iwata H, Sato D, Nakamura Y, Arai S, Monji Y, Kuwayama T

    The 2nd World Congress on Reproductive Biology  2011年10月 

    　詳細を見る

    記述言語：英語   会議種別：ポスター発表  

    researchmap

  • 体外成熟培地へのアミノ酸がブタ卵子の直径変化とエネルギー生産に及ぼす影響.

    田崎秀尚, 岩田尚孝, 門司恭典, 桑山岳人

    第104回日本繁殖生物学会大会  2011年9月 

    　詳細を見る

    記述言語：日本語   会議種別：ポスター発表  

    researchmap

▼全件表示

受賞

  • 優秀演題賞

    2023年5月   第64回日本卵子学会学術集会  

    　詳細を見る

    受賞区分：国内学会・会議・シンポジウム等の賞 

    researchmap

  • Young Investigator Award

    2018年4月   Asia Pacific Initiative on Reproduction 2018  

    　詳細を見る

  • 学術奨励賞論文部門

    2016年5月   日本卵子学会  

    　詳細を見る

共同研究・競争的資金等の研究

  • 卵巣微小環境の老化分子機序の解明と抗加齢療法の開発

    研究課題/領域番号：22K16879  2022年04月 - 2025年03月

    日本学術振興会  科学研究費助成事業 若手研究  若手研究

    田崎 秀尚

    　 詳細を見る

    配分額：4550000円 （ 直接経費：3500000円 、 間接経費：1050000円 ）

    researchmap

  • 哺乳類卵におけるストレスに対する応答機構の解明

    2021年10月 - 2022年09月

    公益財団法人両備檉園記念財団  研究助成金 

    田﨑秀尚

    　 詳細を見る

    担当区分：研究代表者 

    researchmap

  • 老化がマウス卵母細胞の微小環境を構成する体細胞のトランスクリプトームに及ぼす影響

    2021年10月 - 2022年03月

    東京農業大学生物資源ゲノム解析センター  2021年度後期共同研究 

    田﨑秀尚

    　 詳細を見る

    担当区分：研究代表者 

    researchmap

  • 遺伝子発現プロファイル解析に基づいたヒト卵子体外培養系の構築と妊孕性温存への応用

    研究課題/領域番号：20K18221  2020年04月 - 2022年03月

    日本学術振興会  科学研究費助成事業 若手研究  若手研究

    田崎 秀尚

    　 詳細を見る

    配分額：4160000円 （ 直接経費：3200000円 、 間接経費：960000円 ）

    researchmap

 

担当授業科目

  • 生殖補助医療技術実習Ⅱ （2024年度） 特別  - その他

  • 生殖補助医療技術実習Ⅲ （2024年度） 夏季集中  - その他

  • 生殖補助医療技術実習Ｉ （2024年度） １～４学期  - その他

 

社会貢献活動

  • 生殖補助医療技術ってなに？

    役割：講師,　実演

    岡山県立芳泉高等学校  2023年12月25日

    　詳細を見る

  • 生殖補助医療技術ってなに？

    役割：講師,　実演

    愛媛県立今治西高等学校  2023年12月18日

    　詳細を見る

    種別：出前授業

    researchmap

  • 受精卵の状態を見てみよう

    役割：講師

    岡山大学病院リプロダクションセンター 「第19回不妊・不育とこころの講演会」不妊・不育治療の実際  2023年8月26日

    　詳細を見る

    種別：講演会

    researchmap

  • 生殖補助医療技術ってなに？

    役割：講師

    和歌山県立信愛高校  2023年7月13日

    　詳細を見る

    種別：出前授業

    researchmap

  • 生殖補助医療技術入門

    役割：講師,　実演

    岡山県立津山高等学校 「メディカルサイエンスⅡ」ワークショップ  2023年6月24日

    　詳細を見る

    種別：出前授業

    researchmap

  • 胚培養士の仕事

    役割：講師

    島根県立出雲高等学校  PDGｚセミナー  2023年3月14日

    　詳細を見る

    種別：出前授業

    researchmap

  • 生殖「医療」と「研究」

    役割：講師

    岡山県立岡山芳泉高等学校  2022年10月29日

    　詳細を見る

    種別：出前授業

    researchmap

  • 受精卵の状態を見てみよう

    役割：講師

    岡山大学病院リプロダクションセンター  「第18回不妊・不育とこころの講演会」不妊・不育治療の実際  2022年7月30日

    　詳細を見る

    種別：講演会

    researchmap

  • 胚培養士と生殖医療分野の研究について

    役割：講師

    山口県立防府高等学校  2022年7月27日

    　詳細を見る

    種別：出前授業

    researchmap

  • 生殖補助医療技術入門

    役割：講師,　実演

    岡山県立津山高等学校 「メディカルサイエンスⅡ」ワークショップ  2022年6月25日

    　詳細を見る

    種別：出前授業

    researchmap

  • 生命のはじまり「受精卵」をみてみよう

    役割：講師,　実演

    岡山大学  令和3年度「岡山大学公開講座」  2021年10月23日

    　詳細を見る

    種別：施設一般公開

    researchmap

  • 受精卵の状態を見てみよう

    役割：講師

    岡山大学病院リプロダクションセンター  「第１７回不妊・不育とこころの講演会」不妊・不育治療の実際  2021年8月7日

    　詳細を見る

    種別：セミナー・ワークショップ

    researchmap

  • 生殖補助医療技術入門

    役割：講師,　実演

    岡山県立津山高等学校 「メディカルサイエンスⅡ」第２回ワークショップ  2021年8月4日

    　詳細を見る

    種別：出前授業

    researchmap

▼全件表示