2022/05/02 更新

写真a

タサキ ヒデタカ
田﨑 秀尚
TASAKI Hidetaka
所属
生殖補助医療技術教育研究センター 助教
職名
助教
外部リンク

学位

  • 博士(畜産学) ( 東京農業大学 )

研究キーワード

  • 繁殖生物学

  • 卵巣

  • 生殖細胞

  • 生殖医療

  • 卵母細胞

研究分野

  • ライフサイエンス / 動物生命科学

  • ライフサイエンス / 産婦人科学

経歴

  • 岡山大学   学術研究院環境生命科学学域   助教(兼担)

    2021年4月 - 現在

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  • 岡山大学   生殖補助医療技術教育研究センター   助教

    2019年7月 - 現在

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  • 岡山大学   大学院環境生命科学研究科   助教(兼担)

    2019年7月 - 2021年3月

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論文

  • The effect of betaine for mouse sperm cryopreservation 査読

    Natsuho Mori, Moeka Ishihara, Hidetaka Tasaki, Tadashi Sankai, Junko Otsuki

    Cryobiology   2022年4月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier {BV}  

    DOI: 10.1016/j.cryobiol.2022.03.006

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  • Effect of docosahexaenoic acid on in vitro growth of bovine oocytes 査読

    Shuta Nagata, Kaoru Tatematsu, Hitoki Yamaguchi, Yuki Inoue, Keisuke Tanaka, Hidetaka Tasaki, Koumei Shirasuna, Hisataka Iwata

    Reproductive Medicine and Biology   20 ( 4 )   485 - 493   2021年10月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1002/rmb2.12403

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    その他リンク: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/rmb2.12403

  • Nuclear-to-cytoplasmic ratios of 1PN and 2PN zygotes after in vitro fertilization of mouse oocytes 査読 国際共著

    Natsumi Okajima, Wei Xiao, Alex Lopata, Tadashi Sankai, Lubna Yasmin, Yasushi Nagai, Ryota Okamoto, Hidetaka Tasaki, Junko Otsuki

    Zygote   1 - 5   2021年6月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Cambridge University Press (CUP)  

    <title>Summary</title>
    Numerous studies have reported comparisons of the nuclear-to-cytoplasmic (NC) ratio during mitosis. However, little information is known about how the pronuclear size is regulated and determined at the end of meiosis II in mammalian zygotes. The present study aims to analyze the NC ratio of female and male pronuclei, and also to compare the size of single pronuclei using photographs that were obtained during experiments to create chimeric hermaphrodites from 2-cell oocytes. The volume of both the female and the male pronucleus was found to correlate with the volume of the oocyte cytoplasm. The NC ratio of the male pronucleus was greater than that of the female pronucleus. The NC ratio of the average volume of the female and male pronuclei was greater than the NC ratio of the mononucleate oocytes. The occurrence of 1PN oocytes was significantly higher when the volume of cytoplasm was lower than the cut-off value. These results indicated that the NC ratio is retained during pronuclear formation. A higher NC ratio in male compared with the female pronucleus indicated structural and/or molecular difference between the two pronuclei. 1PN formation may occur when sperm enters close to the MII spindle.

    DOI: 10.1017/s096719942100040x

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  • Upregulation of CHOP participates in caspase activation and virus release in human astrovirus-infected cells 査読

    Tomoyasu Isobe, Shoichiro Tange, Hidetaka Tasaki, Kumiko Kanamori, Akiko Kato, Akira Nakanishi

    Journal of General Virology   100 ( 5 )   778 - 792   2019年5月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Microbiology Society  

    DOI: 10.1099/jgv.0.001250

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  • Relationship between the number of cells surrounding oocytes and energy states of oocytes 査読

    Yasuhisa Munakata, Tomoya Ichinose, Kaori Ogawa, Nobuhiko Itami, Hidetaka Tasaki, Koumei Shirasuna, Takehito Kuwayama, Hisataka Iwata

    THERIOGENOLOGY   86 ( 7 )   1789 - 1798   2016年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    Lipid content, ATP content, and histone acetylation are thought to reflect the energy state of cells. In addition, the energy state closely associates with growth and developmental ability of oocytes. Oocyte growth is accompanied by active proliferation of the surrounding granulosa cells (GCs), and GCs play a key role in the provision of energy substrates to the oocytes. In the present study, we first examined the relationship among the average number of GCs per follicle, the average number of cumulus cells (CCs) per oocyte, and the average lipid content in oocytes that developed in vivo within individual donor gilts. Second, we validated the relationship between the number of cells surrounding oocytes and the energy states of oocytes by using an IVC system of oocyte granulosa cell complexes (OGCs) derived from early antral follicles. We collected cumulus cells and oocyte complexes (COCs) from antral follicles (3-5 mm in diameter) and found that average lipid content in oocytes significantly correlated with the average number of both GCs/follicle and CCs/oocyte (P &lt; 0.05). In the next series of experiments, we collected OGCs from early antral follicles (0.5-0.7 mm in diameter), and cultured them for 14 days, and then determined the cell number of OGCs, as well as the lipid content, ATP content, and acetylation level of H4K12 in oocytes grown in vitro. In addition, glucose consumption by OGCs was calculated from the sample media collected at Days 13 and 14. The lipid content of oocytes grown in vitro, significantly correlated with the number of cells surrounding the oocytes (P &lt; 0.01) and with the level of glucose consumption by OGCs (P &lt; 0.01). In addition, both ATP content and H4K12 acetylation levels of oocytes grown in vitro significantly correlated with the number of cells surrounding the oocytes (P &lt; 0.05) and glucose consumption by OGCs (P &lt; 0.05). In conclusion, the lipid content of oocytes depends on the number of cells surrounding the oocytes, and glucose uptake by OGCs is crucial for lipid content and ATP content, and H4K12 acetylation in oocytes. (C) 2016 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.theriogenology.2016.05.036

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  • Gene expression patterns in granulosa cells and oocytes at various stages of follicle development as well as in in vitro grown oocyte-and-granulosa cell complexes 査読

    Yasuhisa Munakata, Ryoka Kawahara-Miki, Shogo Shiratsuki, Hidetaka Tasaki, Nobuhiko Itami, Koumei Shirasuna, Takehito Kuwayama, Hisataka Iwata

    JOURNAL OF REPRODUCTION AND DEVELOPMENT   62 ( 4 )   359 - 366   2016年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOCIETY REPRODUCTION & DEVELOPMENT-SRD  

    Follicle development is accompanied by proliferation of granulosa cells and increasing oocyte size. To obtain high-quality oocytes in vitro, it is important to understand the processes that occur in oocytes and granulosa cells during follicle development and the differences between in vivo and in vitro follicle development. In the present study, oocytes and granulosa cells were collected from early antral follicles (EAFs, 0.5-0.7 mm in diameter), small antral follicles (SAFs, 1-3 mn in diameter), large antral follicles (LAFs, 3-7 mm in diameter), and in vitro grown oocyte-and-granulosa cell complexes (OGCs), which were cultured for 14 days after collection from EAFs. Gene expression was analyzed comprehensively using the next-generation sequencing technology. We found top upstream regulators during the in vivo follicle development and compared them with those in in vitro developed OGCs. The comparison revealed that HIFI is among the top regulators during both in vivo and in vitro development of OGCs. In addition, we found that HIF1-mediated upregulation of glycolysis in granulosa cells is important for the growth of OGCs, but the cellular metabolism differs between in vitro and in vivo grown OGCs. Furthermore, on the basis of comparison of upstream regulators between in vivo and in vitro development of OGCs, we believe that low expression levels of FLT1 (VEGFA receptor), SPP1, and PCSK6 can be considered causal factors of the suboptimal development under in vitro culture conditions.

    DOI: 10.1262/jrd.2016-022

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  • Examination of a plasmid-based reverse genetics system for human astrovirus 査読

    Benoit Chapellier, Shoichiro Tange, Hidetaka Tasaki, Kazuhiro Yoshida, Yan Zhou, Naomi Sakon, Kazuhiko Katayama, Akira Nakanishi

    MICROBIOLOGY AND IMMUNOLOGY   59 ( 10 )   586 - 596   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    A plasmid-based reverse genetics system for human astrovirus type 1 (HAstV1) is examined. Upon transfection into 293T cells, the plasmid vector, which harbors a HAstV1 expression cassette, expressed astroviral RNA that appeared to be capable of viral RNA replication, as indicated by the production of subgenomic RNA and capsid protein expression irrespective of the heterologous 5 ends of the transcribed RNA. Particles infectious to Caco-2 cells were made in this system; however, their infectivity was much lower than would be expected from the amount of particles apparently produced. Using Huh-7 cells as the transfection host with the aim of improving viral capsid processing for virion maturation partially restored the efficiency of infectious particle formation. Our results support the possibility that the DNA transfection process induces a cellular response that targets late, but not early, stages of HAstV1 infection.

    DOI: 10.1111/1348-0421.12317

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  • Effects of reaggregated granulosa cells and oocytes derived from early antral follicles on the properties of oocytes grown in vitro 査読

    Ayano Oi, Hidetaka Tasaki, Yasuhisa Munakata, Koumei Shirasuna, Takehito Kuwayama, Hisataka Iwata

    JOURNAL OF REPRODUCTION AND DEVELOPMENT   61 ( 3 )   191 - 197   2015年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOCIETY REPRODUCTION & DEVELOPMENT-SRD  

    In this study, we examined the effects of reconstructed oocyte granulosa cell complexes (OGCs) on the development of porcine oocytes derived from early antral follicles (EAFs; 0.5-0.7 mm in diameter). When denuded oocytes were cocultured with granulosa cells derived from other EAFs, the oocytes and granulosa cells aggregated to form OGCs after 2 days of culture. After 14 days of culture, we compared cell number, oocyte diameter, and oocyte chromatin configuration in unmanipulated (natural) OGCs, reconstructed OGCs, and OGCs collected from antral follicles (AFs, 3.0-6.0 mm in diameter). The diameters of oocytes from reconstructed OGCs grown in vitro were not different from those of oocytes from natural OGCs, although they were significantly smaller than those of oocytes from antral follicle (AF) OGCs. Oocyte chromatin configuration did not differ among the 3 OGC groups, but the oocyte nuclear maturation rate was lower in the reconstructed OGCs and higher in the AF OGCs. However, when the in vitro culture period for the reconstructed OGCs was extended by 2 days, the nuclear maturation rate of oocytes from reconstructed OGCs was similar to that of oocytes from natural OGCs. In addition, blastocysts were successfully obtained from oocytes from reconstructed OGCs. In conclusion, we established an innovative culture method that allows oocytes and granulosa cells from EAFs to reaggregate as reconstructed OGCs, which yield oocytes with the ability to develop to the blastocyst stage.

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  • The Effect of High Glucose Concentration on the Quality of Oocytes Derived from Different Growth Stages of Follicles 査読

    Tasaki H, Munakata Y, Arai S, Murakami S, Kuwayama T, Iwata H

    Journal of Mammalian Ova Research   32 ( 1 )   41 - 48   2015年

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    記述言語:英語  

    DOI: 10.1274/jmor.32.41

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  • Relationship between mitochondrial DNA Copy Number and SIRT1 Expression in Porcine Oocytes 査読

    Daichi Sato, Nobuhiko Itami, Hidetaka Tasaki, Shun Takeo, Takehito Kuwayama, Hisataka Iwata

    PLOS ONE   9 ( 4 )   e94488   2014年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    The present study assessed the effect of resveratrol on the expression of SIRT1 and mitochondrial quality and quantity in porcine oocytes. Supplementing the maturation medium with 20 mu M resveratrol increased the expression of SIRT1, and enhanced mitochondrial functions, as observed from the increased ATP content and mitochondrial membrane potential. Addition of resveratrol also improved the ability of oocytes to develop into the blastocyst stage following activation. The effects of resveratrol on mitochondrial number were examined by comparing the mitochondrial DNA copy number (Mt number) between group of oocytes collected from the same donor gilt ovaries. Supplementing the maturation medium with only resveratrol did not affect the Mt number in the oocytes. However, supplementing the maturation medium with 10 mu M MG132, a proteasome inhibitor, significantly increased the amount of ubiquitinated proteins and Mt number by 12 and 14%, respectively. In addition, when resveratrol was added to the medium containing MG132, the Mt number increased significantly by 39%, this effect was diminished by the addition of the SIRT1 inhibitor EX527. Furthermore, supplementing the medium with MG132 and EX527 did not affect Mt number. The mean SIRT1 expression in 20 oocytes was significantly and positively correlated with the Mt number in oocytes collected from the same donor. This study suggests that the expression of SIRT1 is associated with the Mt number in oocytes. In addition, activation of SIRT1 by resveratrol enhances the biosynthesis and degradation of mitochondria in oocytes, thereby replenishing and improving mitochondrial function and the developmental ability of oocytes.

    DOI: 10.1371/journal.pone.0094488

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  • Estradiol has a major role in antrum formation of porcine preantral follicles cultured in vitro 査読

    Hidetaka Tasaki, Hisataka Iwata, Daichi Sato, Yasunori Monji, Takehito Kuwayama

    THERIOGENOLOGY   79 ( 5 )   809 - 814   2013年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    Antrum formation and estradiol (E2) secretion occur during early folliculogenesis. The objective was to determine the role of E2 in antrum formation of oocyte-granulosa cell complexes (OGCs) derived from porcine preantral follicles (PAFs). Supplementation of the culture medium with E2 (1 mu g/mL) improved antrum formation of OGCs during 14 days of in vitro culture. Furthermore, adding 0.1 mu g/mL androstenedione (a precursor of E2) to the medium also improved antrum formation. Concentration of E2 was higher in the medium of developmentally competent OGCs versus incompetent OGCs (8.5 vs. 3.5 ng/mL, P &lt; 0.05). Fulvestrant (1 mu g/mL), a competitive inhibitor of E2, completely inhibited antrum formation of OGCs that were cultured in medium containing either E2 (0.1 mu g/mL) or androstenedione (0.1 mu g/mL); however, increasing E2 to 1 mu g/mL ameliorated the inhibitory effect. Conversely, in the case of early antral follicles, OGCs formed antrums without E2 supplementation. After E2 pretreatment, OGCs derived from PAFs formed antrums even when the OGCs were subsequently cultured in medium without E2. Furthermore, when OGCs derived from PAFs were cultured without E2 followed by an additional in vitro culture with E2, antrums were formed, albeit with the same period delay by the same pretreatment periods. In conclusion, E2 in the culture medium was indispensable for in vitro antrum formation of OGCs derived from PAFs; therefore, one of the roles of E2 is in the initiation of antrum formation. (c) 2013 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.theriogenology.2012.12.009

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▼全件表示

MISC

  • Acquisition of meiotic competence in porcine secondary follicles following long-term culture

    Hidetaka Tasaki, Nobuhiko Itami, Hisataka Iwata

    Reproduction Abstracts   2014年9月

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    出版者・発行元:Bioscientifica  

    DOI: 10.1530/repabs.1.p141

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受賞

  • 柳町隆造賞

    2021年10月   第24回日本IVF学会学術集会  

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  • 優秀演題賞

    2021年10月   第6回せとうちART研究会  

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  • Young Investigator Award

    2018年4月   Asia Pacific Initiative on Reproduction 2018  

    田﨑 秀尚

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  • 2016年度学術奨励賞論文部門

    2016年   日本卵子学会  

    田﨑 秀尚

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共同研究・競争的資金等の研究

  • 卵巣微小環境の老化分子機序の解明と抗加齢療法の開発

    研究課題/領域番号:22K16879  2022年04月 - 2025年03月

    日本学術振興会  科学研究費助成事業 若手研究  若手研究

    田崎 秀尚

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    配分額:4550000円 ( 直接経費:3500000円 、 間接経費:1050000円 )

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  • 遺伝子発現プロファイル解析に基づいたヒト卵子体外培養系の構築と妊孕性温存への応用

    研究課題/領域番号:20K18221  2020年04月 - 2022年03月

    日本学術振興会  科学研究費助成事業 若手研究  若手研究

    田崎 秀尚

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    配分額:4160000円 ( 直接経費:3200000円 、 間接経費:960000円 )

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社会貢献活動

  • 生命のはじまり「受精卵」をみてみよう

    役割:講師

    岡山大学  令和3年度「岡山大学公開講座」  2021年10月23日

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  • 受精卵の状態を見てみよう

    役割:講師

    岡山大学病院リプロダクションセンター  「第17回不妊・不育とこころの講演会」不妊・不育治療の実際  2021年8月7日

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    種別:セミナー・ワークショップ

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