Faculty Profiles - HISANO Hiroshi

Books - HISANO Hiroshi

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Targeted Modification of Grain Dormancy Genes in Barley.

Hiroshi Hisano, Robert E Hoffie, Jochen Kumlehn, Kazuhiro Sato

2024

Language：English

Transgenesis technologies, such as overexpression or RNA interference-mediated suppression, have often been used to alter the activity of target genes. More recently developed targeted genome modification methods using customizable endonucleases allow for the regulation or knockout mutation of target genes without the necessity of integrating recombinant DNA. Such approaches make it possible to create novel alleles of target genes, thereby significantly contributing to crop improvement. Among these technologies, the Cas9 endonuclease-based method is widely applied to several crops, including barley (Hordeum vulgare). In this chapter, we describe an Agrobacterium-based approach to the targeted modification of grain dormancy genes in barley using RNA-guided Cas9 nuclease.

DOI： 10.1007/978-1-0716-3965-8_14

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Genome Editing to Produce Knockout Mutations of Seed Dormancy Genes in Wheat.

Fumitaka Abe, Yoko Kamiya, Yuji Ishida, Hiroshi Hisano, Kanako Kawaura, Toshihiko Komari, Kazuhiro Sato

2024

Language：English

Knockout mutants provide definitive information about the functions of genes related to agronomic traits, including seed dormancy. However, it takes many years to produce knockout mutants using conventional techniques in polyploid plants such as hexaploid wheat. Genome editing with sequence-specific nucleases is a promising approach for obtaining knockout mutations in all targeted homoeologs of wheat simultaneously. Here, we describe a procedure to produce a triple recessive mutant in wheat via genome editing. This protocol covers the evaluation of gRNA and Agrobacterium-mediated transformation to obtain edited wheat seedlings.

DOI： 10.1007/978-1-0716-3965-8_13

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ゲノム編集によるムギ類種子休眠性の改良（ゲノム編集技術）

久野裕, 加星光子, 安倍史高, 佐藤和広（ Role： Contributor , pp183-192）

株式会社情報機構 2023.1 （ ISBN:9784865022421 ）

オオムギの標的変異（植物のゲノム編集実験プロトコール）

久野裕（ Role： Contributor）

化学同人 2022.12 （ ISBN:9784759820881 ）

オオムギの形質転換およびゲノム編集（ひとりではじめる植物バイオテクノロジー入門 : 組織培養からゲノム編集まで）

久野裕（ Role： Contributor）

国際文献社 2022.3 （ ISBN:9784910603032 ）

Doubled Haploid Technology

Robert Eric Hoffie, Ingrid Otto, Hiroshi Hisano, Jochen Kumlehn（ Role： Contributor , Site-Directed Mutagenesis in Barley Using RNA-Guided Cas Endonucleases During Microspore-Derived Generation of Doubled Haploids.）

Springer 2021.7

オオムギのゲノム編集

久野裕（ Role： Contributor）

アグリバイオ 2017.1

Biofuels

Hisano H, Nandakumar R, Wang ZY（ Role： Contributor , Genetic modification of lignin biosynthesis for improved biofuel production）

Springer 2010

マメ科植物の比較ゲノム（ゲノムから読み解く生命システム）

佐藤修正, 久野裕, 田畑哲之（ Role： Contributor）

細胞工学別冊 2007

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