2022/01/28 更新

写真a

クボ ヤスタカ
久保 康隆
KUBO Yasutaka
所属
環境生命科学学域 教授
職名
教授
外部リンク

学位

  • 博士(農学) ( 京都大学 )

  • 農学修士 ( 京都大学 )

  • 博士(農学) ( 京都大学 )

研究キーワード

  • 1-MCP

  • エチレン

  • 軟化

  • 日持ち

  • ethylene

  • fruit ripening

  • softening

研究分野

  • 環境・農学 / 園芸科学

学歴

  • 京都大学    

    - 1983年

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    国名: 日本国

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  • 京都大学   Graduate School, Division of Agriculture  

    - 1983年

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  • 京都大学   Faculty of Agriculture  

    - 1981年

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    国名: 日本国

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  • 京都大学   Faculty of Agriculture  

    - 1981年

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経歴

  • - 岡山大学自然科学研究科 教授

    2004年

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  • - Professor,Graduate School of Natural Science and Technology,Okayama University

    2004年

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所属学協会

  • 日本植物生理学会

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  • 米国園芸学会(American Society for Horticultural Science)

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  • 日本食品低温保蔵学会

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  • 園芸学会

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委員歴

  • 園芸学会   平成21年度秋季大会座長  

    2009年   

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    団体区分:学協会

    園芸学会

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  • 園芸学会   編集幹事  

    2009年   

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    団体区分:学協会

    園芸学会

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  • 園芸学会   平成21年度春季大会座長  

    2008年   

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    団体区分:学協会

    園芸学会

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論文

  • Combined signal sequence trap and macroarray analysis identifies genes associated with differential fruit softening characteristics during ripening in European and Chinese pears

    Mercy W. Mwaniki, Oscar W. Mitalo, Eric G. Mworia, Willis O. Owino, Kyoko Hiwasa-Tanase, Jocelyn K.C. Rose, Koh Aoki, Tomoya Esumi, Takashi Kawai, Ryohei Nakano, Koichiro Ushijima, Yasutaka Kubo

    Postharvest Biology and Technology   174   111436 - 111436   2021年4月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.postharvbio.2020.111436

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  • Low temperature modulates natural peel degreening in lemon fruit independently of endogenous ethylene

    Oscar W. Mitalo, Takumi Otsuki, Rui Okada, Saeka Obitsu, Kanae Masuda, Yuko Hojo, Takakazu Matsuura, Izumi C. Mori, Daigo Abe, William O. Asiche, Takashi Akagi, Yasutaka Kubo, Koichiro Ushijima

    Journal of Experimental Botany   71 ( 16 )   4778 - 4796   2020年8月

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    掲載種別:研究論文(学術雑誌)  

    © 2020 Oxford University Press. All rights reserved. Peel degreening is an important aspect of fruit ripening in many citrus fruit, and previous studies have shown that it can be advanced by ethylene treatment or by low-temperature storage. However, the important regulators and pathways involved in natural peel degreening remain largely unknown. To determine how natural peel degreening is regulated in lemon fruit (Citrus limon), we studied transcriptome and physiochemical changes in the flavedo in response to ethylene treatment and low temperatures. Treatment with ethylene induced rapid peel degreening, which was strongly inhibited by the ethylene antagonist, 1-methylcyclopropene (1-MCP). Compared with 25 ºC, moderately low storage temperatures of 5-20 °C also triggered peel degreening. Surprisingly, repeated 1-MCP treatments failed to inhibit the peel degreening induced by low temperature. Transcriptome analysis revealed that low temperature and ethylene independently regulated genes associated with chlorophyll degradation, carotenoid metabolism, photosystem proteins, phytohormone biosynthesis and signalling, and transcription factors. Peel degreening of fruit on trees occurred in association with drops in ambient temperature, and it coincided with the differential expression of low temperature-regulated genes. In contrast, genes that were uniquely regulated by ethylene showed no significant expression changes during on-tree peel degreening. Based on these findings, we hypothesize that low temperature plays a prominent role in regulating natural peel degreening independently of ethylene in citrus fruit.

    DOI: 10.1093/jxb/eraa206

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  • 海上輸送によるカキ‘中谷早生’の東南アジア輸出時の軟化抑制技術の確立 査読

    古田貴裕, 和中 学, 熊本昌平, 池永裕一, 西銘玲子, 河井 崇, 深松陽介, 福田文夫, 久保康隆, 中野龍平

    園芸学研究   2020年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

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  • Passe Crassane’ pear fruit (Pyrus communis L.) ripening: revisiting the role of low temperature via integrated physiological and transcriptome analysis 査読

    Oscar Witere MITALO, Yasuaki Tosa, Sumire Tokiwa, Yuki Kondo, Azimullah Azimi, Yuko Hojo, Takakazu Matsuura, Izumi Mori, Ryohei NAKANO, Takashi Akagi, Koichiro Ushijima, Yasutaka Kubo

    Postharvest Biology and Technology   2019年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.postharvbio.2019.110949

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  • Low temperature storage stimulates fruit softening and sugar accumulation without ethylene and aroma volatile production in kiwifruit 査読

    Oscar W. Mitalo, Sumire Tokiwa, Yuki Kondo, Takumi Otsuki, Ivan Galis, Katsuhiko Suezawa, Ikuo Kataoka, Anh T. Doan, Ryohei Nakano, Koichiro Ushijima, Yasutaka Kubo

    Frontiers in Plant Science   10   1 - 15   2019年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3389/fpls.2019.00888

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  • Aberrant Stamen Development is Associated with Parthenocarpic Fruit Set through Up-regulation of Gibberellin Biosynthesis in Tomato 査読

    Okabe, Yoshihiro, Yamaoka, Tatsuya, Ariizumi, Tohru, Ushijima, Koichiro, Kojima, Mikiko, Takebayashi, Yumiko, Sakakibara, Hitoshi, Kusano, Miyako, Shinozaki, Yoshihito, Pulungan, Sri I, Kubo, Yasutaka, Nakano, Ryohei, Ezura, Hiroshi

    Plant & Cell Physiology   60 ( 1 )   38 - 51   2019年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Parthenocarpy, a process in which fruit set occurs without fertilization, leads to the production of seedless fruit. A number of floral homeotic mutants with abnormal stamen development exhibit parthenocarpic fruit set. Flower development is thought to repress ovary growth before anthesis. However, the mechanism of parthenocarpic fruit development caused by aberrant flower formation is poorly understood. To investigate the molecular mechanism of parthenocarpic fruit development in floral homeotic mutants, we performed functional analysis of Tomato APETALA3 (TAP3) by loss-of-function approaches. Organ-specific promoter was used to induce organ-specific loss of function in stamen and ovary/fruit. We observed increased cell expansion in tap3 mutants and TAP3-RNAi lines during parthenocarpic fruit growth. These were predominantly accompanied by the up-regulation of GA biosynthesis genes, including SlGA20ox1, SlGA20ox2, and SlGA20ox3, as well as reduced expression of the GA-inactivating gene SlGA2ox1 and the auxin signaling gene SlARF7 involved in a crosstalk between GA and auxin. These transcriptional profiles are in agreement with the GA levels in these lines. These results suggest th

    DOI: 10.1093/pcp/pcy184

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  • Comparative analysis of fruit ripening and associated genes in two kiwifruit cultivars ('Sanuki Gold' and 'Hayward') at various storage temperatures 査読

    Mitalo Oscar Witere, Asiche William Olubero, Kasahara Yuka, Tosa Yasuaki, Tokiwa Sumire, Ushijima Koichiro, Nakano Ryohei, Kubo Yasutaka

    POSTHARVEST BIOLOGY AND TECHNOLOGY   147   20 - 28   2019年1月

  • Determination of optimum temperature for long-term storage and analysis of ripening-related genes in 'Rainbow Red' kiwifruit 査読

    Mitalo, O.W, Tokiwa, S, Kasahara, Y, Tosa, Y, Kondo, Y, Asiche, W.O, Kataoka, I, Suezawa, K, Ushijima, K, Nakano, R, Kubo, Y

    Acta Hortic.   1218   517 - 524   2018年10月

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    掲載種別:研究論文(国際会議プロシーディングス)  

    DOI: 10.17660/ActaHortic.2018.1218.71

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  • Characterization of Ripening-related Genes Involved in Ethylene-independent Low Temperature-modulated Ripening in 'Rainbow Red' Kiwifruit during Storage and On-vine 査読

    Mitalo Oscar Witere, Asiche William Olubero, Kasahara Yuka, Tosa Yasuaki, Owino Willis Omondi, Mworia Eric Gituma, Ushijima Koichiro, Nakano Ryohei, Kubo Yasutaka

    HORTICULTURE JOURNAL   87 ( 3 )   421 - 429   2018年7月

  • Comparative transcriptome analysis reveals distinct ethylene-independent regulation of ripening in response to low temperature in kiwifruit 査読

    Asiche William O, Mitalo Oscar W, Kasahara Yuka, Tosa Yasuaki, Mworia Eric G, Owino Willis O, Ushijima Koichiro, Nakano Ryohei, Yano Kentaro, Kubo Yasutaka

    BMC PLANT BIOLOGY   18   2018年3月

  • Nondestructive Detection of Split Pit in Peaches Using an Acoustic Vibration Method 査読

    Nakano, Ryohei, Akimoto, Hidemi, Fukuda, Fumio, Kawai, Takashi, Ushijima, Koichiro, Fukamatsu, Yosuke, Kubo, Yasutaka, Fujii, Yuichiro, Hirano, Ken, Morinaga, Kunihisa, Sakurai, Naoki

    HORTICULTURE JOURNAL   87 ( 2 )   281 - 287   2018年

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC HORTICULTURAL SCI  

    Split-pit in peach fruit is a problematic disorder. Split-pit fruit cannot be detected based on external appearance, and contamination of fruit by split-pit reduces its reliability in the marketplace. Here, we demonstrate that split-pit fruit can be identified by a nondestructive acoustic vibration method and a unique approach based on the ratio of the third (f(3)) to the second (f(2)) resonant frequency. The response-resonant frequency spectra showed that the peaks of f(2 )frequencies in split-pit fruit were shifted to much lower values than those in normal fruit, whereas those of f(3) frequencies showed only small shifts. The calculated f(3)/f(2 )ratios in most normal fruit were in the range of 1.35-1.4, whereas those in split-pit fruit were 1.45-2.0. Analysis of more than 300 fruit samples revealed that by setting the f(3)/f(2) cut-off value at >1.45, 95% of split-pit fruit in the fruit samples were detected, whereas only 1.5% of normal fruit were missorted as split-pit fruit. A model for simulating the vibration properties of peach fruit was developed by using the finite element method. The simulated vibration patterns showed that f(3)/f(2) values were increased by the insertio

    DOI: 10.2503/hortj.OKD-094

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  • Effect of Storage Temperature on Fruit Ripening in Three Kiwifruit Cultivars

    William Olubero Asiche, Oscar Witere Mitalo, Yuka Kasahara, Yasuaki Tosa, Eric Gituma Mworia, Koichiro Ushijima, Ryohei Nakano, Yasutaka Kubo

    HORTICULTURE JOURNAL   86 ( 3 )   403 - 410   2017年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC HORTICULTURAL SCI  

    The responses of three kiwifruit cultivars, Actinidia chinensis 'Sanuki Gold', A. chinensis 'Rainbow Red', and A. deliciosa 'Hayward' to various storage temperatures (0, 5, 10, 15, and 20 degrees C) for 8 weeks were investigated. The rate of fruit which initiated ethylene production due to rot development increased with increases in storage temperature. Early-maturing cultivars, 'Rainbow Red' and 'Sanuki Gold' fruit stored at 5, 10, and 15 degrees C showed drastic softening and a decrease in titratable acidity (TA) to an edible level within 4 weeks without detectable ethylene production, whereas fruit stored at 0 and 20 degrees C maintained high firmness and TA even after 8 weeks unless they were infected with rot. A late-maturing cultivar, 'Hayward' fruit stored at 5 and 10 degrees C softened more rapidly than when stored at 0, 15, or 20 degrees C. Treatment with 1-Methylcyclopropene (1-MCP) did not suppress the low temperature modulated fruit ripening in any cultivars, indicating its independence from ethylene. These results suggest that 'Sanuki Gold' and 'Rainbow Red' are more sensitive to low temperatures compared to 'Hayward' and the sensitivity is involved in the determination of storage life and how early the fruit matures on the vine.

    DOI: 10.2503/hortj.OKD-028

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  • TOMATOMICS: A Web Database for Integrated Omics Information in Tomato

    Toru Kudo, Masaaki Kobayashi, Shin Terashima, Minami Katayama, Soichi Ozaki, Maasa Kanno, Misa Saito, Koji Yokoyama, Hajime Ohyanagi, Koh Aoki, Yasutaka Kubo, Kentaro Yano

    PLANT AND CELL PHYSIOLOGY   58 ( 1 )   784 - 795   2017年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Solanum lycopersicum (tomato) is an important agronomic crop and a major model fruit-producing plant. To facilitate basic and applied research, comprehensive experimental resources and omics information on tomato are available following their development. Mutant lines and cDNA clones from a dwarf cultivar, Micro-Tom, are two of these genetic resources. Large-scale sequencing data for ESTs and full-length cDNAs from Micro-Tom continue to be gathered. In conjunction with information on the reference genome sequence of another cultivar, Heinz 1706, the Micro-Tom experimental resources have facilitated comprehensive functional analyses. To enhance the efficiency of acquiring omics information for tomato biology, we have integrated the information on the Micro-Tom experimental resources and the Heinz 1706 genome sequence. We have also inferred gene structure by comparison of sequences between the genome of Heinz 1706 and the transcriptome, which are comprised of Micro-Tom full-length cDNAs and Heinz 1706 RNA-seq data stored in the KaFTom and Sequence Read Archive databases. In order to provide large-scale omics information with streamlined connectivity we have developed and maintain a web database TOMATOMICS (http://bioinf.mind.meiji.ac.jp/tomatomics/). In TOMATOMICS, access to the information on the cDNA clone resources, full-length mRNA sequences, gene structures, expression profiles and functional annotations of genes is available through search functions and the genome browser, which has an intuitive graphical interface.

    DOI: 10.1093/pcp/pcw207

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  • Two Members of the Aluminum-Activated Malate Transporter Family, SlALMT4 and SlALMT5, are Expressed during Fruit Development, and the Overexpression of SlALMT5 Alters Organic Acid Contents in Seeds in Tomato (Solanum lycopersicum)

    Takayuki Sasaki, Yoshiyuki Tsuchiya, Michiyo Ariyoshi, Ryohei Nakano, Koichiro Ushijima, Yasutaka Kubo, Izumi C. Mori, Emi Higashiizumi, Ivan Galis, Yoko Yamamoto

    PLANT AND CELL PHYSIOLOGY   57 ( 11 )   2367 - 2379   2016年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    The aluminum-activated malate transporter (ALMT) family of proteins transports malate and/or inorganic anions across plant membranes. To demonstrate the possible role of ALMT genes in tomato fruit development, we focused on SlALMT4 and SlALMT5, the two major genes expressed during fruit development. Predicted proteins were classified into clade 2 of the family, many members of which localize to endomembranes. Tissue-specific gene expression was determined using transgenic tomato expressing the beta-glucuronidase reporter gene controlled by their own promoters. Both the genes were expressed in vascular bundles connecting to developing seeds in fruit and in the embryo of mature seeds. Further, SlALMT5 was expressed in embryo in developing seeds in fruit. Subcellular localization of both proteins to the endoplasmic reticulum (ER) was established by transiently expressing the green fluorescent protein fusions in plant protoplasts. SlALMT5 probably localized to other endomembranes as well. Localization of SlALMT5 to the ER was also confirmed by immunoblot analysis. The transport function of both SlALMT proteins was investigated electrophysiologically in Xenopus oocytes. SlALMT5 transported malate and inorganic anions such as nitrate and chloride, but not citrate. SlALMT4 also transported malate, but the results were less consistent perhaps because it did not localize strongly to the plasma membrane. To elucidate the physiological role of SlALMT5 further, we over-expressed SlALMT5 in tomato. Compared with the wild type, overexpressors exhibited higher malate and citrate contents in mature seeds, but not in fruit. We conclude that the malate transport function of SlALMT5 expressed in developing fruit influences the organic acid contents in mature seeds.

    DOI: 10.1093/pcp/pcw157

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  • Extension of Shelf-life by Limited Duration of Propylene and 1-MCP Treatments in Three Kiwifruit Cultivars

    William Olubero Asiche, Eric Gituma Mworia, Chisato Oda, Oscar Witere Mitalo, Willis Omondi Owino, Koichiro Ushijima, Ryohei Nakano, Yasutaka Kubo

    HORTICULTURE JOURNAL   85 ( 1 )   76 - 85   2016年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC HORTICULTURAL SCI  

    In order to extend the "eating window", the optimum ripening phase suitable for eating, the combination of treatment with propylene (an ethylene analog) and 1-methylcyclopropene (1-MCP; an ethylene inhibitor) was assessed in three kiwifruit cultivars: 'Rainbow Red' Actinidia chinensis, 'Sanuki Gold' A. chinensis, and 'Hayward' A. deliciosa. Propylene treatment initiated the ripening process by inducing fruit softening, increasing soluble solid content (SSC), and decreasing titratable acids (TA), with or without endogenous ethylene production, depending on the duration of exposure. Once endogenous ethylene was induced, it accelerated fruit ripening, resulting in an over-ripening phase and shortening of the "eating window". 'Rainbow Red' and 'Sanuki Gold' fruit treated with propylene continuously or for 48 h initiated endogenous ethylene production that led to an "eating window" lasting only 2 days (range of 3-5 days after the start of treatment), whereas it lasted for 7 days (range 3-10 days) in 'Hayward' fruit. Limited propylene treatment of the three cultivars for 24 h induced ripening without the detection of ethylene production, suggesting that the optimum ripening phase suitable for eating can be attained without endogenous ethylene production, resulting in a longer "eating window". 'Rainbow Red' and 'Sanuki Gold' fruit treated with propylene for 48 h followed by 1-MCP treatment had extended "eating window" and shelf-life, with the suppression of endogenous ethylene. These results illustrate the practicability of different durations of propylene treatment in facilitating kiwifruit ripening and the additional benefit of 1-MCP treatment to the extend shelf-life of new high-quality kiwifruit cultivars, 'Rainbow Red' and 'Sanuki Gold'.

    DOI: 10.2503/hortj.MI-066

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  • 収穫後の近赤外光照射が数種葉菜類の蒸散,気孔開度および外観品質に及ぼす影響

    15 ( 2 )   197 - 206   2016年

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.2503/hrj.15.197

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  • Effect of postharvest short-term radiation of near infrared light on transpiration of lettuce leaf 査読

    Kozuki Ayako, Ishida Yutaka, Kakibuchi Kazumasa, Mishima Toshihiro, Sakurai Naoki, Murata Yoshiyuki, Nakano Ryohei, Ushijima Koichiro, Kubo Yasutaka

    POSTHARVEST BIOLOGY AND TECHNOLOGY   108   78 - 85   2015年10月

  • Genetic Control of Floral Morph and Petal Pigmentation in Linum grandiflorum Desf., a Heterostylous Flax 査読

    Ushijima Koichiro, Ikeda Kazuo, Nakano Ryohei, Matsubara Miyo, Tsuda Yuri, Kubo Yasutaka

    HORTICULTURE JOURNAL   84 ( 3 )   261 - 268   2015年7月

  • Plant Omics Data Center: An Integrated Web Repository for Interspecies Gene Expression Networks with NLP-Based Curation

    Hajime Ohyanagi, Tomoyuki Takano, Shin Terashima, Masaaki Kobayashi, Maasa Kanno, Kyoko Morimoto, Hiromi Kanegae, Yohei Sasaki, Misa Saito, Satomi Asano, Soichi Ozaki, Toru Kudo, Koji Yokoyama, Koichiro Aya, Keita Suwabe, Go Suzuki, Koh Aoki, Yasutaka Kubo, Masao Watanabe, Makoto Matsuoka, Kentaro Yano

    PLANT AND CELL PHYSIOLOGY   56 ( 1 )   2015年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Comprehensive integration of large-scale omics resources such as genomes, transcriptomes and metabolomes will provide deeper insights into broader aspects of molecular biology. For better understanding of plant biology, we aim to construct a next-generation sequencing (NGS)-derived gene expression network (GEN) repository for a broad range of plant species. So far we have incorporated information about 745 high-quality mRNA sequencing (mRNA-Seq) samples from eight plant species (Arabidopsis thaliana, Oryza sativa, Solanum lycopersicum, Sorghum bicolor, Vitis vinifera, Solanum tuberosum, Medicago truncatula and Glycine max) from the public short read archive, digitally profiled the entire set of gene expression profiles, and drawn GENs by using correspondence analysis (CA) to take advantage of gene expression similarities. In order to understand the evolutionary significance of the GENs from multiple species, they were linked according to the orthology of each node (gene) among species. In addition to other gene expression information, functional annotation of the genes will facilitate biological comprehension. Currently we are improving the given gene annotations with natural language processing (NLP) techniques and manual curation. Here we introduce the current status of our analyses and the web database, PODC (Plant Omics Data Center; http://bioinf.mind.meiji.ac.jp/podc/), now open to the public, providing GENs, functional annotations and additional comprehensive omics resources.

    DOI: 10.1093/pcp/pcu188

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  • 収穫後の貯蔵温度,形態がエダマメ‘ふくら’と‘湯あがり娘’の品質に及ぼす影響

    14 ( 3 )   297 - 304   2015年

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.2503/hrj.14.297

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  • エダマメ‘ふくら’と‘湯あがり娘’における生育中の品質関連因子解析に基づく収穫適期の評価

    14 ( 1 )   61 - 67   2015年

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.2503/hrj.14.61

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  • Double flower formation induced by silencing of C-class MADS-box genes and its variation among petunia Cultivars 査読

    Siti Hajar Noor, Koichiro Ushijima, Ayaka Murata, Kaori Yoshida, Miki Tanabe, Tomoki Tanigawa, Yasutaka Kubo, Ryohei Nakano

    SCIENTIA HORTICULTURAE   178   1 - 7   2014年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Double flowers induced by virus-induced gene silencing (VIGS) of two C-class MADS-box genes, pMADS3 and FBP6, were investigated in four cultivars of Petunia hybrida. In flowers induced by either pMADS3-VIGS or FBP6-VIGS, only small changes in commercial appearance were recognized regardless of cultivar, whereas in those induced by pMADS3/FBP6-VIGS, complete conversion of stamens into petaloid tissues and marked enlargement of upper limb-like tissues were observed, resulting in a decorative appearance in all the four cultivars. In whorl 4, cultivar-dependent conversion of carpels into new flowers was noted in pMADS3/FBP6-VIGS flowers. Of the four cultivars, only 'Mambo Purple' exhibited the development of new flowers instead of carpels and the emergence of ectopic new flowers from the axil of whorl 3 organs, which created an ornamental appearance with a high commercial value. Investigation of large and small petaloid stamens induced by pMADS3/FBP6-VIGS and pMADS3-VIGS, respectively, revealed only small differences in cell size compared to the large difference in total surface area. Quantitative RT-PCR analysis of SQUAMOSA/AP1/FRU type A-class genes, FBP29, PFG, and FBP26, showed no close relationship between the expression of those genes and petaloid stamen size. The suppressed C-class gene function at the late stage of flower development has little influence on the final size of petaloid tissue. (C) 2014 Elsevier IV. All rights reserved.

    DOI: 10.1016/j.scienta.2014.07.029

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  • Characterization of ripening-associated genes using a tomato DNA macroarray, 1-methylcyclopropene, and ripening-impaired mutants

    Rui Yan, Naoki Yokotani, Tatsuya Yamaoka, Kohichiro Ushijima, Ryohei Nakano, Kentaro Yano, Koh Aoki, Yasutaka Kubo

    POSTHARVEST BIOLOGY AND TECHNOLOGY   86   159 - 170   2013年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Using a tomato (Solanum lycopersicum) DNA macroarray consisting of 11,520 genes, we identified 419 ripening-associated genes (224 upregulated, 195 downregulated). Treatment with 1-methylcyclopropene (1-MCP) at the turning stage restored expression to levels of the mature green (MG) stage in 159 upregulated and 45 downregulated genes, suggesting a strong dependence on ethylene signaling during ripening. Among 194 ethylene-upregulated genes, 143 genes in ripening inhibitor (rin) fruit and 140 genes in non-ripening (nor) fruit responded poorly to exogenous ethylene, suggesting the necessity of RIN and NOR signals for their expression. In contrast, 36 and 70 genes responded to ethylene in rin and nor fruit, respectively, to a similar extent as in wild-type fruit, suggesting a clear independence of RIN and NOR signals for their response to ethylene. Ripening-associated expression of number of genes involved in respiratory, lipid and energy metabolism, and ethylene were upregulated during ripening under strong control of an ethylene signal. Large number of photosynthesis-related genes were downregulated during ripening in an ethylene-independent manner, whereas decreased expression of a few genes such as chloroplast thiazole biosynthetic protein and phosphoenolpyruvate carboxylase, was overturned by 1-MCP treatment, suggesting ethylene dependent regulation. Ripening-associated expression of several transcription and signal transduction factors, such as TDR4, GRAS, S-adenosyl-L-homocysteine hydrolase, BNK1, bZip, and BTB showed clear ethylene dependency, suggesting their involvement in regulation of fruit ripening downstream of ethylene signaling. (C) 2013 The Authors. Published by Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.postharvbio.2013.06.028

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  • Low-temperature-modulated fruit ripening is independent of ethylene in 'Sanuki Gold' kiwifruit

    Eric G. Mworia, Takashi Yoshikawa, Nadiah Salikon, Chisato Oda, William O. Asiche, Naoki Yokotani, Daigo Abe, Koichiro Ushijima, Ryohei Nakano, Yasutaka Kubo

    JOURNAL OF EXPERIMENTAL BOTANY   63 ( 2 )   963 - 971   2012年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Fruit ripening in response to treatments with propylene, 1-methycyclopropene (1-MCP), and low temperature was characterized in 'Sanuki Gold' kiwifruit, Actinidia chinensis Planch. Propylene treatment immediately induced rapid fruit softening, increased AC-PG (polygalacturonase) and AC-EXP (expansin) mRNA accumulation, and stimulated an increase in the soluble solid concentration (SSC) and a decrease in titratable acidity (TA). After 3 d exposure to propylene, ethylene production and AC-PL (pectate lyase) mRNA accumulation were observed. 1-MCP treatment after 24 h exposure to propylene eliminated AC-PG mRNA accumulation and suppressed continued changes in SSC and TA. Application of 1-MCP at the start of the treatment, followed by continuous propylene exposure, markedly delayed fruit softening, and the expression of the cell wall-modifying genes, and changes in the SSC and TA, indicating that kiwifruit become insensitive to ethylene at least for 3 d following 1-MCP exposure. Surprisingly, significant fruit softening, mRNA accumulation of AC-PG, AC-PL, and AC-EXP, and decreased TA were observed without ethylene production in intact fruit stored at low temperature for 1 month, but not in fruit stored at room temperature. Repeated 1-MCP treatments (twice a week) failed to inhibit the changes that occurred in low temperature storage. These observations indicate that low temperature modulates the ripening of kiwifruit in an ethylene-independent manner, suggesting that kiwifruit ripening is inducible by either ethylene or low temperature signals.

    DOI: 10.1093/jxb/err324

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  • Isolation of the floral morph-related genes in heterostylous flax (Linum grandiflorum): the genetic polymorphism and the transcriptional and post-transcriptional regulations of the S locus 査読

    Ushijima Koichiro, Nakano Ryohei, Bando Mayu, Shigezane Yukari, Ikeda Kazuo, Namba Yuko, Kume Saori, Kitabata Toshiyuki, Mori Hitoshi, Kubo Yasutaka

    PLANT JOURNAL   69 ( 2 )   317 - 331   2012年1月

  • Effect of MA Storage and 1-MCP on Storability and Quality of 'Sanuki Gold' Kiwifruit Harvested at Two Different Maturity Stages 査読

    Eric Gituma Mworia, Takashi Yoshikawa, Nadiah Salikon, Chisato Oda, Tetsuo Fukuda, Katsuhiko Suezawa, William Olubero Asiche, Koichiro Ushijima, Ryohei Nakano, Yasutaka Kubo

    JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE   80 ( 3 )   372 - 377   2011年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC HORTICULTURAL SCI  

    The effects of modified atmosphere (MA) storage and application of 1-methylcyclopropene (1-MCP) at harvest on the storability and quality of 'Sanuki Gold' kiwifruit harvested at two different maturity stages were investigated. MA storage in both fruit harvested early at 136 days after pollination (DAP) or late at 154 DAP delayed flesh softening, increase in soluble solid concentrations (SSC), decrease in titratable acids (TA), and reduction in fruit flesh color index compared to air stored fruit, suggesting that MA storage is effective in prolonging 'Sanuki Gold' kiwifruit storage life. Further, MA stored fruit did not attain full ripening flesh firmness and SSC thresholds even after 4 months of storage under MA conditions, suggesting that early harvested 'Sanuki Gold' kiwifruit can be stored for 4 months in MA. Fruit from both harvesting maturity stages stored under air conditions achieved maximum SSC (18%) values during storage, suggesting that two weeks early harvesting did not compromise edible quality characteristics. Only late harvested fruit treated with 1-MCP and stored in MA showed slight inhibitory effect specific to fruit softening during the first and second month of storage, suggesting that 1-MCP may have some limited ripening inhibitory effect during storage of 'Sanuki Gold' kiwifruit.

    DOI: 10.2503/jjshs1.80.372

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  • Comparative Analysis of Differential Gene Expression in Wild-type and 12C5+ Ion Beam-induced Abnormal Flower Mutant of Solanum villosum by Tomato cDNA Macroarray

    Ojiewo, C, Kubo Y, Murakami K, Masuda

    Int. J. Plant Dev. Biol.   4   1 - 7   2011年

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  • Characterization of ethylene biosynthesis and its regulation during fruit ripening in kiwifruit, Actinidia chinensis ‘Sanuki Gold’

    E.G. Mworiaa, T. Yoshikawaa, N. Yokotanic, T. Fukudab, K. Suezawab, K. Ushijimaa, R. Nakanoa, Y. Kubo

    Postharvest Biology and Technology   55 ( 2 )   108 - 113   2010年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.postharvbio.2009.08.007

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  • A fraction of unripe kiwi fruit extract regulates adipocyte differentiation and function in 3T3-L1 cells

    Daigo Abe, Takeshi Saito, Yasutaka Kubo, Yoshimasa Nakamura, Keizo Sekiya

    BIOFACTORS   36 ( 1 )   52 - 59   2010年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Adipocyte dysfunction is strongly associated with the development of insulin resistance and diabetes, and regulation of adipogenesis is important in prevention of diabetes. We prepared a 100% methanol fraction of methanolic extract from unripe kiwi fruit (Actinidia deliciosa), designated KMF (kiwi fruit methanol fraction) When applied to 3T3-L1 preadipocyte cells, KMF promoted adipocyte differentiation, increased glycerol-3-phosphate dehydrogenase (GPDH) activity, and increased triglyceride (TG) content. KMF markedly increased mRNA expression of peroxisome proliferator-activated receptor gamma (PPAR gamma)-the master adipogenic transcription factor-and its target genes. Moreover, KMF increased mRNA expression and protein secretion of adiponectin, whereas mRNA expression and secretion of monocyte chemoattractant protein-1 (MCP-1) and interleukin-6 (IL-6) were decreased. Compared with troglitazone, KMF decreased the production of reactive oxygen species (ROS) and nuclear factor-kappaB (NF kappa B) activation. Glucose uptake was stimulated by KMF in differentiated 3T3-L1 adipocytes. Taken together, these results indicate that KMF may exert beneficial effects against diabetes via its ability to regulate adipocyte differentiation and function. (C) 2010 International Union of Biochemistry and Molecular Biology, Inc.

    DOI: 10.1002/biof.70

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  • Utilization Immature Macadamia Nuts in Prevention of Thermal Decomposition of Polyunsaturated Fatty Acids (PUFA): Case study with Docosahexaenoic Acid (DHA)

    Karanja P. N, Mwasaru M. A, Koaze H, Kubo Y, Baba N

    Trop. Agri. Dev.   54   140 - 142   2010年

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  • Ripening-associated ethylene biosynthesis in tomato fruit is autocatalytically and developmentally regulated

    Naoki Yokotani, Ryohei Nakano, Shunsuke Imanishi, Masayasu Nagata, Akitsugu Inaba, Yasutaka Kubo

    JOURNAL OF EXPERIMENTAL BOTANY   60 ( 12 )   3433 - 3442   2009年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    To investigate the regulatory mechanism(s) of ethylene biosynthesis in fruit, transgenic tomatoes with all known LeEIL genes suppressed were produced by RNA interference engineering. The transgenic tomato exhibited ethylene insensitivity phenotypes such as non-ripening and the lack of the triple response and petiole epinasty of seedlings even in the presence of exogenous ethylene. Transgenic fruit exhibited a low but consistent increase in ethylene production beyond 40 days after anthesis (DAA), with limited LeACS2 and LeACS4 expression. 1-Methylcyclopropene (1-MCP), a potent inhibitor of ethylene perception, failed to inhibit the limited increase in ethylene production and expression of the two 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS) genes in the transgenic fruit. These results suggest that ripening-associated ethylene (system 2) in wild-type tomato fruit consists of two parts: a small part regulated by a developmental factor through the ethylene-independent expression of LeACS2 and LeACS4 and a large part regulated by an autocatalytic system due to the ethylene-dependent expression of the same genes. The results further suggest that basal ethylene (system 1) is less likely to be involved in the transition to system 2. Even if the effect of system 1 ethylene is eliminated, fruit can show a small increase in ethylene production due to unknown developmental factors. This increase would be enough for the stimulation of autocatalytic ethylene production, leading to fruit ripening.

    DOI: 10.1093/jxb/erp185

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  • ナシ果実における二次元電気泳動による細胞壁画分タンパク質の解析

    坂東麻由, 牛島幸一郎, 中野龍平, 稲葉昭次, 久保康隆

    日本食品保蔵科学会誌   34 ( 3 )   127 - 132   2008年

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  • Differential expression of ethylene biosynthetic genes in climacteric and non-climacteric Chinese pear fruit

    Miki Yamane, Daigo Abe, Sayaka Yasui, Naoki Yokotani, Wataru Kimata, Koichiro Ushijima, Ryohei Nakano, Yasutaka Kubo, Akitsugu Inaba

    POSTHARVEST BIOLOGY AND TECHNOLOGY   44 ( 3 )   220 - 227   2007年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    We investigated the differences in capability to produce ripening-associated ethylene between climacteric ('Yali', 'Xinqingli', and 'Zhuzuili') and non-climacteric ('Hongli', 'Yuanbali', and 'Hongxiaoli') Chinese pear (Pyrus bretschneideri Rehder) fruit varieties. Three ACS (PbACS1, PbACS2, and PbACS3), two ACO (PbACO1 and PbACO2), and three MADS-box (PbMADS1, PbMADS2, and PbMADS3) genes were cloned from ripening fruit. Fruit were harvested at the mature stage and treated with 5000 mu L L-1 propylene for 4 days. Ethylene production was induced by propylene in the climacteric but not in non-climacteric type fruit. In the ripening climacteric fruit, PbACS1 and PbACO1 transcript accumulation accompanied ethylene production but the accumulation of other ACS and ACO mRNAs was not detected. In 'Yali' fruit, 1-MCP exposure prior to propylene treatment completely inhibited the expression of these genes, while exposure after the commencement of ethylene production weakened their expression. Transcripts of PbACO1 accumulated in response to propylene treatment even in non-climacteric fruit but this accumulation was eliminated after the termination of propylene treatment. In response to wounding, transcripts of PbACS2, PbACS3, and PbACO2 genes accumulated in both climacteric and non-climacteric fruit, but accumulation of PbACS1 and PbACO1 mRNAs was not detected. In the Southern analysis of PbACS1, HindIII digests of genomic DNA showed 8.3, 3.5 and 2.9 kb bands. The 2.9 kb band was detected only in climacteric varieties while the 3.5 kb band was detected in both climacteric and non-climacteric varieties except in 'Yali'. These results suggest that Chinese pears may have two copies of the ACSI gene, in which PbACS1A could be linked to the varietal differences in the capability to produce ripening-associated ethylene. There was no correlation between the expression patterns of the three MADS-box genes cloned and the differences in ripening-associated ethylene production among the Chinese pear varieties. (C) 2006 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.postharvbio.2006.12.010

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  • Ethylmethanesulfonate (EMS) mutagenesis of Solanum lycopersicum cv. Micro-Tom for large-scale mutant screens

    Shin Watanabe, Tsuyoshi Mizoguchi, Koh Aoki, Yasutaka Kubo, Hitoshi Mori, Shunsuke Imanishi, Yukiko Yamazaki, Daisuke Shibata, Hiroshi Ezura

    PLANT BIOTECHNOLOGY   24 ( 1 )   33 - 38   2007年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY  

    Sequencing a whole genome, cataloguing full-length cDNAs and ESTs, and construction of a comprehensive mutant population are essential steps in genome projects of individual model species. Tomato is one of the most important model crops to be undertaken for study over the next few decades, and the Solanaceae Genomics Project (SOL) has begun genome projects as international collaborations. The Solanum lycopersicum cultivar Micro-Tom shares several advantages with Arabidopsis, including its small size, short life cycle (approximately 90 days), and growth normally under artificial light. Here we demonstrate an initial attempt to generate a comprehensive mutant population in Micro-Tom. A total of 3,839 M2 families derived from ethylmethanesulfonate (EMS) mutagenesis were visually phenotyped, and putative mutants were classified into 15 primary and 48 secondary categories based on the SOL database, "The Genes That Make Tomatoes".

    DOI: 10.5511/plantbiotechnology.24.33

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  • beta-galactosidase and alpha-L-arabinofuranosidase activities and gene expression in European and Chinese pear fruit during ripening

    Mercy W. Mwaniki, Francis M. Mathooko, Kyoko Hiwasa, Akira Tateishi, Naoki Yokotani, Koichiro Ushijima, Ryohei Nakano, Akitsugu Inaba, Yasutaka Kubo

    JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE   76 ( 1 )   85 - 90   2007年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC HORTICULTURAL SCI  

    Substantial decreases in cell wall bound galactosyl and arabinosyl residues are two of the most evident cell wall compositional changes that occur during fruit ripening. The roles of P-galactosidase (P-Gal) and alpha-L-arabinofuranosidase (alpha-Af), the enzymes responsible for these respective losses, were investigated and compared in European (Pyrus communis L. 'La France') and Chinese (Pyrus bretschneideri Rehd. 'Yali') pear fruits which exhibit different softening characteristics during ripening. The increase in the activities of P-Gal and a-Af during ripening in both types of pear fruit correlated well with an increase in climacteric ethylene production, and a concomitant decrease in flesh firmness in 'La France' fruit. However, there was no noticeable decrease in 'Yali' flesh firmness even after 28 days of storage at room temperature. In both fruit types, enzyme activity and the accumulation of transcripts hybridizing with PpGAL1, PpGAL4, PpARF2, and PcARF1 increased with fruit ripening. Increases in gene expression and enzyme activities in 'Yali' fruit with no detectable softening during ripening indicate that P-Gal and a-Af may not mediate difference in fruit softening between two pears, but that they could play some role(s) in cell wall changes, perhaps in cooperation with other cell wall-modifying enzymes such as polygalacturonase.

    DOI: 10.2503/jjshs.76.85

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  • Differential feedback regulation of ethylene biosynthesis in pulp and peel tissues of banana fruit

    Akitsugu Inaba, Xuejun Liu, Naoki Yokotani, Miki Yamane, Wang-Jin Lu, Ryohei Nakano, Yasutaka Kubo

    JOURNAL OF EXPERIMENTAL BOTANY   58 ( 5 )   1047 - 1057   2007年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    The feedback regulation of ethylene biosynthesis in banana [Musa sp. (AAA group, Cavendish subgroup) cv. Grand Nain] fruit was investigated in an attempt to clarify the opposite effect of 1-methylcyclopropene (1-MCP), an ethylene action inhibitor, before and after the onset of ripening. 1-MCP pre-treatment completely prevented the ripening-induced effect of propylene in pre-climacteric banana fruit, whereas treatment after the onset of ripening stimulated ethylene production. In pre-climacteric fruit, higher concentrations of propylene suppressed ethylene production more strongly, despite their earlier ethylene-inducing effect. Exposure of the fruit ripened by propylene to 1-MCP increased ethylene production concomitantly with an increase in 1-aminocyclopropane-1-carboxylate (ACC) synthase activity and ACC content, and prevented a transient decrease in MA-ACS1 transcripts in the pulp tissues. In contrast, in the peel of ripening fruit, 1-MCP prevented the increase in ethylene production and subsequently the ripening process by reduction of the increase in MA-ACS1 and MA-ACO1 transcripts and of ACC synthase and ACC oxidase activities. These results suggest that ethylene biosynthesis in ripening banana fruit may be controlled negatively in the pulp tissue and positively in the peel tissue. This differential regulation by ethylene in pulp and peel tissues was also observed for MA-PL, MA-Exp, and MA-MADS genes.

    DOI: 10.1093/jxb/erl265

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  • Ethylene regulation of fruit softening and cell wall disassembly in Charentais melon

    Kiyomi Nishiyama, Monique Guis, Jocelyn K. C. Rose, Yasutaka Kubo, Kristen A. Bennett, Wangjin Lu, Kenji Kato, Koichiro Ushijima, Ryohei Nakano, Akitsugu Inaba, Mondher Bouzayen, Alain Latche, Jean-Claude Pech, Alan B. Bennett

    JOURNAL OF EXPERIMENTAL BOTANY   58 ( 6 )   1281 - 1290   2007年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Cell wall disassembly in ripening fruit is highly complex, involving the dismantling of multiple polysaccharide networks by diverse families of wall-modifying proteins. While it has been reported in several species that multiple members of each such family are expressed in the same fruit tissue, it is not clear whether this reflects functional redundancy, with protein isozymes from a single enzyme class performing similar roles and contributing equally to wall degradation, or whether they have discrete functions, with some isoforms playing a predominant role. Experiments reported here sought to distinguish between cell wall-related processes in ripening melon that were softening-associated and softening-independent. Cell wall polysaccharide depolymerization and the expression of wall metabolism-related genes were examined in transgenic melon (Cucumis melo var. cantalupensis Naud.) fruit with suppressed expression of the 1-aminocyclopropane-1-carboxylate oxidase (ACO) gene and fruits treated with ethylene and 1-methylcyclopropene (1-MCP). Softening was completely inhibited in the transgenic fruit but was restored by treatment with exogenous ethylene. Moreover, post-harvest application of 1-MCP after the onset of ripening completely halted subsequent softening, suggesting that melon fruit softening is ethylene-dependent. Size exclusion chromatography of cell wall polysaccharides, from the transgenic fruits, with or without exogenous ethylene, indicated that the depolymerization of both pectins and xyloglucans was also ethylene dependent. However, northern analyses of a diverse range of cell wall-related genes, including those for polygalacturonases, xyloglucan endotransglucosylase/hydrolases, expansin, and beta-galactosidases, identified specific genes within single families that could be categorized as ethylene-dependent, ethylene-independent, or partially ethylene-dependent. These results support the hypothesis that while individual cell wall-modifying proteins from each family contribute to cell wall disassembly that accompanies fruit softening, other closely related family members are regulated in an ethylene-independent manner and apparently do not directly participate in fruit softening.

    DOI: 10.1093/jxb/erl283

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  • Regulatory mechanisms of ethylene biosynthesis in response to various stimuli during maturation and ripening in fig fruit (Ficus carica L.)

    W. O. Owino, Y. Manabe, F. M. Mathooko, Y. Kubo, A. Inaba

    PLANT PHYSIOLOGY AND BIOCHEMISTRY   44 ( 5-6 )   335 - 342   2006年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER  

    In order to obtain a greater uniformity of maturation, the growth of the fig fruit (Ficus carica L.) can be stimulated by the application of either olive oil, ethrel/ethephon or auxin. The three treatments induce ethylene production in figs. In this study, we investigated the regulatory mechanisms responsible for oil, auxin and ethylene induced ethylene production in figs. The ethylene production in response to olive oil, auxin, and propylene treatments and during ripening were all induced by 1-methylcyclopropene (1-MCP) and inhibited by propylene indicating a negative feedback regulation mechanism. Three 1-aminocyclopropane-1-carboxylic acid (ACC) synthase genes (Fc-ACS1, Fc-ACS2 and Fc-ACS3) and one ACC oxidase gene (Fc-ACO1) were isolated and their expression patterns in response to either oil, propylene or auxin treatment in figs determined. The expression patterns of Fc-ACS1 and Fc-ACO1 were clearly inhibited by 1-MCP and induced by propylene in oil treated and ripe fruits indicating positive regulation by ethylene, whereas Fc-ACS2 gene expression was induced by 1-MCP and inhibited by propylene indicating negative regulation by ethylene. The Fc-ACS3 mRNA showed high level accumulation in the auxin treated fruit. The inhibition of Fc-ACS3 gene by 1-MCP in oil treated and in ripe fruits suggests that auxin and ethylene modulate the expression of this gene by multi-responsive signal transduction pathway mechanisms. We further report that the olive oil-induced ethylene in figs involves the ACC-dependent pathway and that multiple ethylene regulatory pathways are involved during maturation and ripening in figs and each specific pathway depends on the inducer/stimulus. (c) 2006 Elsevier SAS. All rights reserved.

    DOI: 10.1016/j.plaphy.2006.03.009

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  • Expression characteristics of seven members of the β-galactosidase gene family in 'La France' pear (Pyrus communis L.) fruit during growth and their regulation by 1-methylcyclopropene during postharvest ripening.

    Mwaniki M. W, F. M. Mathooko, M. Matsuzakia, K. Hiwasac, A. Tateishi, K. Ushijimae, R. Nakanoe, A. Inabae, Y. Kubo

    Postharvet. Biol. Technol.   36 ( 3 )   253 - 263   2005年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.postharvbio.2005.02.002

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  • Cloning and expression analysis of an XET cDNA in the peel and pulp of banana during fruit ripening and softening.

    Lu, W-J, Nakano, R, Kubo, Y, Inaba, A, Jiang, Y-M

    Acta Botanica Sinica   2005年

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  • Differential Expression of Members of the β-Galactosidase Gene Family during Japanese Pear (Pyrus pyrifolia L.) Fruit Growth and On-tree Ripening.

    Tateishi, A, K. Nagashima, F. M. Mathooko, M. W. Mwaniki, Y. Kubo, A. Inaba, S. Yamaki, H. Inoue

    J. Amer. Soc. Hort. Sci.   2005年

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  • European, Chinese and Japanese pear fruits exhibit differential softening characteristics during ripening

    K Hiwasa, R Nakano, A Hashimoto, M Matsuzaki, H Murayama, A Inaba, Y Kubo

    JOURNAL OF EXPERIMENTAL BOTANY   55 ( 406 )   2281 - 2290   2004年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Softening characteristics were investigated in three types of pear fruit, namely, European pear 'La France', Chinese pear 'Yali', and Japanese pear 'Nijisseiki'. 'La France' fruit softened dramatically and developed a melting texture during ripening, while 'Yali' fruit with and without propylene treatment showed no change in flesh firmness and texture during ripening. Non-treated 'Nijisseiki' did not show a detectable decrease in flesh firmness, whereas continuous propylene treatment caused a gradual decrease in firmness resulting in a mealy texture. In 'La France', the analysis of cell wall polysaccharides revealed distinct solubilization and depolymerization of pectin and hemicellulose during fruit softening. In 'Nijisseiki', propylene treatment led to the solubilization and depolymerization of pectic polysaccharides to a limited extent, but not of hemicellulose. In 'Yali', hemicellulose polysaccharides were depolymerized during ripening, but there was hardly any change in pectic polysaccharides except in the water-soluble fraction. PC-PG1 and PC-PG2, two polygalacturonase (PG) genes, were expressed in 'La France' fruit during ripening, while only PC-PG2 was expressed in 'Nijisseiki' and neither PC-PG1 or PC-PG2 was expressed in 'Yali'. The expression pattern of PC-XET1 was constitutive during ripening in all three pear types. PG activity measured by the reducing sugar assay increased in all three pears during ripening. However, viscometric measurements showed that the levels of endo-PG activity were high in 'La France', low in 'Nijisseiki', and undetectable in 'Yali' fruits. These results suggest that, in pears, cell wall degradation is correlated with a decrease in firmness during ripening and the modification of both pectin and hemicellulose are essential for the development of a melting texture. Furthermore, the data suggest that different softening behaviours during ripening among the three pear fruits may be caused by different endo-PG activity and different expression of PG genes.

    DOI: 10.1093/jxb/erh250

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  • Coordinated expression patterns of genes encoding cell wall modifying enzymes during ripening in distinct anatomical tissue regions of the fig (Ficus carica L.) fruit

    WO Owino, R Nakano, Y Kubo, A Inaba

    POSTHARVEST BIOLOGY AND TECHNOLOGY   32 ( 3 )   253 - 261   2004年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Fig (Fiats carica L.) fruit undergoes rapid softening during ripening, a process that involves changes in the solubility and molecular size of the cell wall polysaccharides in both the receptacle and drupelet tissues. In this study we report on the isolation and characterization of the expression patterns of eleven genes encoding a collection of cell wall modifying enzymes during ripening of the fig fruit. The genes studied include those encoding endo-polygalacturonases (PGs), endo-glucanases (EGases), beta-galactosidases (beta-Gals), xyloglucan endotransglycosylases (XTHs,), expansins (Exps) and alpha-arabinofuranosidases (Arabfs). The Northern analysis data revealed that the expression patterns of these genes in both tissues during fig fruit ripening could be divided into three categories. The Fc-Pg1, Fc-Pg2, Fc-Cel1, Fc-Cel2 and Fc-Gal2 transcripts showed increased accumulation from the ripening onset to the over ripe stage and were clustered into group (I). The Fc-Exp1, Fc-Gal1, Fc-Arabf1, Fc-XTH1 and Fc-XTH2 transcripts accumulated at the immature stage and were clustered into group (II). The Fc-XTH3 transcript accumulated only at the over ripe stage and was the only member of group (III). Other than Fc-PG2 which was specific to the drupelet tissue, all the other genes were detected in both the receptacle and the drupelets tissue albeit at differing stages of ripening and with different intensity of accumulation. This study suggests that the gene products of the 11 isolated cDNAs putatively encoding cell wall related enzymes are coordinated both in time and amount during fig fruit development and ripening and act together in an interdependent way to achieve softening. (C) 2004 Elsevier B.V All rights reserved.

    DOI: 10.1016/j.postharvbio.2004.01.003

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  • Comparison of ethylene- and wound-induced responses in fruit of wild-type, rin and nor tomatoes

    N Yokotani, S Tamura, R Nakano, A Inaba, WB McGlasson, Y Kubo

    POSTHARVEST BIOLOGY AND TECHNOLOGY   32 ( 3 )   247 - 252   2004年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Responses of wild-type, rin, and nor tomato (Lycopersicon esculentunt Mill.) fruit to exogenous ethylene and wounding were compared to identify the events directly controlled by each mutation. When rin and nor fruit were exposed to exogenous ethylene, respiration and accumulation of E4, E8, and LE-ACO1 mRNAs increased to levels similar to those observed in the wild-type fruit, indicating that expression of these genes is independent of either mutations. In contrast, accumulation of LE-ACS4 and PG mRNAs was not restored by exogenous ethylene treatment in both mutants, indicating that the expression of both genes requires both RIN and NOR. Interestingly accumulation of LE-ACS2 mRNA was stimulated by ethylene treatment in rin fruit but not in nor fruit. This suggests that the signaling pathways of RIN and NOR affect the regulation of ripening-associated genes in distinct ways, at least with respect to the expression of LE-ACS2. Wounding induced ethylene biosynthesis and increased accumulation of LE-ACS2, LE-ACS6, LE-ACO1, and E4 mRNAs in both wild-type and the mutants to the same extent. Treatment with 1-methylcyclopropene (1-MCP), a potent inhibitor of ethylene perception, preceding wounding, inhibited the increase of E4 mRNA but did not affect the expression profile of other genes in all the lines tested. These results show that the wounding signal that controls LE-ACS2, LE-ACS6, and LE-ACO1 is independent of rin and nor mutations and ethylene signaling. (C) 2004 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.postharvbio.2004.01.001

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  • Alterations in cell wall polysaccharides during ripening in distinct anatomical tissue regions of the fig (Ficus carica L.) fruit

    WO Owino, R Nakano, Y Kubo, A Inaba

    POSTHARVEST BIOLOGY AND TECHNOLOGY   32 ( 1 )   67 - 77   2004年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    During the last stage of fig (Ficus carica L.) fruit development, profound cell wall modification processes occur as indicated by increase in fruit size and tissue softening. In this study, we characterized the changes in cell wall polysaccharides taking place within the distinct and separate tissues of the receptacle and the Pulpy drupelets during sequential ripening in fig fruit. The pectic extracts had high Uronic acid contents in addition to high amounts of Ara, Gal and Rha. The gel filtration profile of the water-soluble polymers at the ripening onset in drupelets were different when compared to those of the receptacle, even though in both tissues, these polymers underwent increased solubilization and depolymerization during ripening. The molecular downshift of the CDTA-soluble polymers and the decrease in the amounts of both the uronic acid and total sugars were more pronounced in the drupelets than in the receptacle. Major difference in the neutral sugar composition between the two tissues was only observed in the Na2CO3-soluble fraction. The xyloglucan polymers in 4% KOH fraction exhibited a molecular size downshift accompanied by a decline in Xyl and increase in Glc. In the 24% KOH fractions of both tissues, the total sugar and xyloglucan components decreased in amount and also exhibited a molecular size downshift during ripening. These data suggest that even though quantitative and qualitative changes in cell wall polysaccharides occurred during ripening in both tissues, qualitative variations between tissues occurred only in the pectic polymers but not in the hemicellulosic polymers. (C) 2003 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.postharvbio.2003.10.003

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  • Influence of time and concentration of 1-MCP application on the shelf life of pear 'La France' fruit

    Y Kubo, K Hiwasa, WO Owino, R Nakano, A Inaba

    HORTSCIENCE   38 ( 7 )   1414 - 1416   2003年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC HORTICULTURAL SCIENCE  

    'La France' pear (Pyrus communis L.) fruit were exposed to chilling temperature (-1 degreesC) for a week to induce ethylene biosynthesis before they were transferred to 20 degreesC to allow ripening. On 1, 4, or 7 days after the transfer to room temperature, fruit were treated with 20 muL(.)L(-1) 1-MCP for 12 hours. The 1-MCP treatments suppressed ethylene and carbon dioxide production significantly and slowed fruit softening. The shelf life period of fruit with desirable firmness treated with 1-MCP on day 4 was twice that of untreated fruit, with firmness of 1-MCP treated fruit on day I being higher than desirable while that of fruit treated on day 7 was lower than desirable. To determine the optimum 1-MCP concentration for treatment, fruit were exposed to 0.01 to 100 muL(.)L(-1)1-MCP 3 days after the transfer to 20 degreesC. The fruit treated with 1 muL(.)L(-1)1-MCP and less ripened similarly to untreated fruit, having a shelf life of a week. 1-MCP treatments of 10 and 100 muL(.)L(-1) inhibited ethylene and carbon dioxide production, and delayed fruit softening and occurrence of senescent breakdown. The flesh firmness of these fruit maintained suitable eating quality for more than 3 weeks. Our results indicate that 1-MCP treatment of 10 muL(.)L(-1) at 20 degreesC 3 to 4 days after initiation of ripening can extend the shelf life of 'La France' pear fruit. Chemical name used: 1-methylcyclopropene (1-MCP).

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  • Characterization of a novel tomato EIN3-like gene (LeEIL4)

    N Yokotani, S Tamura, R Nakano, A Inaba, Y Kubo

    JOURNAL OF EXPERIMENTAL BOTANY   54 ( 393 )   2775 - 2776   2003年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    A novel cDNA clone encoding a putative EIN3-like protein (LeEIL4) was identified from ripening tomato (Lycopersicon esculentum) fruit. The predicted amino acid sequence contained conserved domains of EIN3-like proteins in the N-terminal half. In phylogenetic analysis, LeEIL4 was classified into the cluster consisting of EIN3 and EIN3-like proteins known to be involved in ethylene signal transduction. LeEIL4 transcripts accumulated in all tissues examined, with higher levels in ripening fruit.

    DOI: 10.1093/jxb/erg308

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  • Extending shelf-life of astringent persimmon (Diospyros kaki Thunb.) fruit by 1-MCP

    S Harima, R Nakano, S Yamauchi, Y Kitano, Y Yamamoto, A Inaba, Y Kubo

    POSTHARVEST BIOLOGY AND TECHNOLOGY   29 ( 3 )   319 - 324   2003年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    We investigated the potential for commercial use of 1-methylcyclopropene (1-MCP) to extend the shelf life of 'Tonewase' and 'Saijo' fruit, Japanese astringent persimmon cultivars, in combination with a de-astringency treatment using high CO2 concentrations. The non-1-MCP treated fruit softened within 5 days after harvest, resulting in unacceptable quality. The 1-MCP treatments at more than 100 nl l(-1) for 16-48 h inhibited fruit softening for 12 and 16 days after harvest at room temperature, for 'Tonewase' and 'Saijo', respectively. Treatment with 10 nl l(-1) 1-MCP had a limited inhibitory effect on softening. A time lag of up to 12 h from harvest to the beginning of 1-MCP treatment did not reduce the beneficial effects of 1-MCP. Fruit treated with 1-MCP remained insensitive to ethylene for 4 days after the end of the treatment. These results indicate that 1-MCP has the potential to extend the shelf life of Japanese persimmons. (C) 2003 Elsevier B.V. All rights reserved.

    DOI: 10.1016/S0925-5214(03)00058-9

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  • Differential expression of seven alpha-expansin genes during growth and ripening of pear fruit

    K Hiwasa, JKC Rose, R Nakano, A Inaba, Y Kubo

    PHYSIOLOGIA PLANTARUM   117 ( 4 )   564 - 572   2003年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL MUNKSGAARD  

    Seven cDNAs, designated PcExp1 to PcExp7 , encoding expansin homologues, were isolated from mature pear fruit and their expression profiles were investigated in ripening fruit and other tissues, and in response to ethylene. Accumulation of PcExp2 , -3, -5 and -6 mRNA increased markedly with fruit softening and then declined at the over-ripe stage. Treatment of fruit at an early ripening stage with 1-methylcyclopropene (MCP), an inhibitor of ethylene action, suppressed ethylene biosynthesis, fruit softening and the accumulation of the expansin mRNAs. Conversely, propylene treatment at the preclimacteric stage induced accumulation of the same four expansin genes, as well as ethylene production and fruit softening. The expression patterns correlated with alteration in the rate and extent of fruit softening. The abundance of PcExp1 mRNA increased at the late expanding phase of fruit development and further increased during ripening, whereas PcExp4 mRNA levels were constant throughout fruit growth and ripening. The MCP and propylene treatments had little effect on PcExp1 and PcExp4 expression. PcExp7 was expressed in young but not mature fruit. PcExp4 and PcExp6 mRNA was also detected in flowers. The accumulation of PcExp4, -5, -6 and -7 mRNA was more abundant in young growing tissues, but not in fully expanded tissues, suggesting roles for these genes in cell expansion. These results demonstrate that characteristically, multiple expansin genes show differential expression and hormonal regulation during pear fruit development and at least six expansins show overlapping expression during ripening.

    DOI: 10.1034/j.1399-3054.2003.00064.x

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  • Ethylene is required for both the initiation and progression of softening in pear (Pyrus communis L.) fruit

    K Hiwasa, Y Kinugasa, S Amano, A Hashimoto, R Nakano, A Inaba, Y Kubo

    JOURNAL OF EXPERIMENTAL BOTANY   54 ( 383 )   771 - 779   2003年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    In order to investigate the physiological role of ethylene in the initiation and subsequent progression of softening, pear fruit were treated with propylene, an analogue of ethylene or 1-methylcyclopropene (1-MCP), a gaseous inhibitor of ethylene action at the preclimacteric or ripening stages. The propylene treatment at the pre-ripe stage stimulated ethylene production and flesh softening while the 1-MCP treatment at the same stage markedly retarded the initiation of the ripening-related events. Moreover, 1-MCP treatment after the initiation of ripening markedly suppressed the subsequent flesh softening and ethylene production. These results clearly indicate that ethylene is not merely a by-product, but plays a crucial role in both the initiation and maintenance of regulating the softening process during ripening. The observations also suggest that ethylene in ripening is regulated entirely in an autocatalytic manner. The mRNA accumulation of pear polygalacturonases (PG) genes, PC-PG1 and PC-PG2, was in parallel with the pattern of fruit softening in both propylene and 1-MCP treatments. However, the expression pattern of pear endo-1,4-beta-D-glucanases (EGase) genes, PC-EG1 and PC-EG2, was not affected in both treatments. The results suggest that ethylene is required for PGs expression even in the late ripening stage, but not for EGases.

    DOI: 10.1093/jxb/erg073

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  • Expression analysis of genes encoding xyloglucan endtransglycosylase during ripening in pear fruit.

    Hiwasa;K, R., Nakano, A., Inaba, Y., Kubo, 棚瀬, 京子

    Acta Horticulturae   628   549-553   2003年

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)  

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  • Involvement of stress-induced ethylene biosynthesis in fruit softening of 'Saijo' persimon fruit 査読

    R Nakano, Y Kubo, A Inaba, S Harima

    PROCEEDINGS OF THE 2ND INTERNATIONAL PERSIMMON SYMPOSIUM   ( 601 )   219 - 226   2003年

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:INTERNATIONAL SOCIETY HORTICULTURAL SCIENCE  

    We investigated the involvement of stress-induced ethylene in softening of 'Saijo' persimmon fruit with or without removal of astringency by holding the fruit in >95% carbon dioxide for 16h at 25degreesC, a technique generally referred to as the Constant Temperature Short Duration (CTSD) treatment. 'Saijo' fruit were harvested at commercial maturity from the research farm of Okayama University, Okayama, Japan. In order to confirm the role of ethylene in fruit softening, fruit were treated with 1-methylcyclopropene (MCP), a strong inhibitor of ethylene perception. In the fruit treated with MCP, softening was inhibited markedly compared with non-treated fruit. To test the effects of water stress and CTSD treatment on fruit softening and ethylene production during shelf-life, we held fruits with or without CTSD treatment in high (>95% RH) or low humidity (40-60%) at 20degreesC. In the fruits without CTSD treatment, high humidity conditions retarded both commencement of ethylene production and fruit softening significantly. However, in CTSD treated fruits, a considerable amount of ethylene was produced and fruit softening proceeded irrespective of humidity. These results indicate that ethylene is involved in fruit softening and that ethylene production is stimulated by not only water stress but also carbon dioxide stress in 'Saijo' persimmon fruit.

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  • Suppression of postharvest softening of forcing-cultured 'Tonewase' Japanese persimmon by packaging in improved carton boxes

    Shinji Harima, Ryohei Nakano, Susumu Yamauchi, Yasutaka Kubo, Akitsugu Inaba, Yoshinobu Kitano

    Journal of the Japanese Society for Horticultural Science   71   583 - 587   2002年8月

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    To establish a suitable package that can suppress rapid postharvest softening of forcing-cultured 'Tonewase' Japanese persimmon, the performance of carton boxes, coated with a water-impervious material, was investigated under experimental and commercial distribution conditions. Packaging fruit in the improved carton boxes kept relative humidity above 70%, effectively reduced weight loss, delayed ethylene induction, and suppressed fruit softening under experimental and commercial conditions, compared to those shipped in conventional carton boxes.

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  • Differential regulation of genes encoding ethylene biosynthesis enzymes and ethylene response sensor ortholog during ripening and in response to wounding in avocados

    WO Owino, R Nakano, Y Kubo, A Inaba

    JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE   127 ( 4 )   520 - 527   2002年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC HORTICULTURAL SCIENCE  

    We investigated the differential regulation of two 1-aminocyclopropane-1-carboxylate synthase (ACS) genes, one 1-aminocyclopropane-1-carboxylate oxidase (ACO) gene and one ethylene response sensor (ERS1) ortholog during ripening and in response to wounding in avocados (Persea americana Mill. 'Bacon'). The 1-aminocyclopropane-1-carboxylate (ACC) content, ACS activity and detectable expression of PA-ACS1 mRNA increased and reached a maximum prior to the climacteric peak, whereas ACO activity and the PA-ACO mRNA levels increased markedly only at the upsurge of ripening ethylene. A basal level of PA-ERS1 transcript was detected as from harvest, however, PA-ERS1 transcript was hyper-induced at the climacteric peak of ethylene production. I-Methylcyclopropene (1-MCP) application at thepreclimacteric and the onset of climacteric stages inhibited the ACS and ACO activities, the transcription of PA-ACS1 and suppressed PA-ACO and PA-ERS1 mRNAs to trace levels. Discontinuation of 1-MCP treatment led to super-induction of PA-ACS1, PA-ACO, and PA-ERS1 transcripts. Wound induced ethylene biosynthesis and wound-induced PA-ACS2 mRNA accumulation were enhanced by 1-MCP, whereas wound-induced PA-ACO mRNA accumulation was unaffected by 1-MCP. These results indicate positive feedback regulation of the PA-ACS1 gene and negative feedback regulation of the PA-ACS2 gene by ethylene, while PA-ACO exhibits positive feedback regulation by ethylene and is also induced by wounding. The hyper-induction of PA-ERS1 mRNA at relatively high concentrations of ethylene may be a mechanism of avocados to regulate the ethylene responsiveness of the tissues by dissipation of the gas.

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  • Water stress-induced ethylene in the calyx triggers autocatalytic ethylene production and fruit softening in 'Tonewase' persimmon grown in a heated plastic-house

    R Nakano, S Inoue, Y Kubo, A Inaba

    POSTHARVEST BIOLOGY AND TECHNOLOGY   25 ( 3 )   293 - 300   2002年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    'Tonewase' Japanese persimmon fruit (Diospyros kaki Thunb.) grown in a heated plastic-house softens rapidly within several days of harvest, which is a major problem in marketing of this cultivar. In this study, we elucidated the involvement of water stress-induced ethylene in fruit softening and investigated the induction mechanism of this ethylene biosynthesis occurring in specific tissues at the molecular level. Two instances of increase in ethylene production were observed in fruit held in ambient low humidity conditions (40-60% RH), an initial increase on the 1st and 2nd days and a second increase on the 6th and 8th days after harvest. Increase in ethylene production was accompanied by rapid softening in these fruit. Fruit held in high humidity conditions (> 95%) neither produced detectable levels of ethylene nor softened rapidly. Moreover, treatment of the fruit held in low humidity with 1-methylcyclopropene (1-MCP), a strong inhibitor of ethylene action, inhibited fruit softening remarkably. These results suggest the involvement of water stress-induced ethylene in fruit softening. 1-MCP also suppressed the second increase in ethylene production but not the initial increase, indicating that the initial increase is induced directly in response to the primary water stress signal while the second is induced autocatalytically by the ethylene produced during the initial phase. During the initial increase in ethylene, the calyx produced more than 5 nl g(-1) h(-1) of ethylene accompanied by increased accumulation of 1-aminocyclopropane-1-carboxylic acid (ACC) and expression of DK-ACS2. In pulp, 0.5 nl g(-1) h(-1) of ethylene was detected but no increase in ACC content or expression of any ethylene biosynthetic genes was observed. During the second increase in ethylene, ethylene production in the calyx was not detected whereas the pulp produced 0.2-0.4 nl g(-1) h(-1) of ethylene with a marked increase in ACC content and expression of the two ACC synthase (DK-ACS1, DK-ACS2) and one ACC oxidase (DK-ACO1) genes. These results suggest that in plastic-house 'Tonewase' persimmon fruit, ethylene production is initiated in the calyx in response to water stress through activated expression of DK-ACS2, and this ethylene in turn induces autocatalytic ethylene production in the pulp. As the flesh firmness decreased markedly just after the initial ethylene production, the results also indicate that the fruit softening is due to the action of ethylene produced in the calyx. (C) 2002 Elsevier Science B.V. All rights reserved.

    DOI: 10.1016/S0925-5214(02)00009-1

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  • Inhibition of fruit softening in forcing-cultured 'Tonewase' Japanese persimmon by packaging in perforated and non-perforated polyethylene bags

    Shinji Harima, Ryohei Nakano, Susumu Yamauchi, Yoshinobu Kitano, Yasutaka Kubo, Akitsugu Inaba, Eiichi Tomita

    Journal of the Japanese Society for Horticultural Science   71   284 - 291   2002年4月

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    To establish an optimum postharvest handling technique to reduce loss during transportation and marketing due to softening of CTSD (Constant Temperature and Short Duration)-treated forcing-cultured 'Tonewase' Japanese persimmon (Diospyros kaki Thunb.), the effect of packaging in polyethylene bags was studied. 1. Whether perforated or not, packaging of 'Tonewase' persimmons in polyethylene bags significantly suppressed fruit softening and weight loss. Fruit maintained their firmness longest in non-perforated bag with 0.1 mm thick film. The difference in the shelf-life between perforated and non-perforated bags was within several days. 2. A slight induction of ethylene production was observed at the end of the CO2 treatment. The rate of ethylene production in unpackaged (control) fruit increased immediately after the end of the CO2 treatment, and resulted in rapid softening; fruit packaged in perforated or pin-holed polyethylene bags evolved a trace level of ethylene without a significant change in softening for 10 days. 3. Astringency in the fruit packaged in perforated or pin-holed polyethylene bags was completely removed within 3 days after CO2 treatment, whereas a significant level of soluble tannin remained in the fruit sealed in the non-perforated polyethylene bag 7 days after CO2 treatment. 4. Because a 6-hr delay in packaging in perforated polyethylene bag after harvest eliminated the beneficial effects of the packaging, we recommend that fruit be packaged immediately after harvest to maximize the shelf-life of 'Tonewase' fruit.

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  • Varietal variation in microsatellite and caps for ethylene- related genes and its possible association with agronomic characters in melon (Cucumis melo L.) 査読

    Akashi, Y, Ezura, H, Kubo, Y, Masuda, M, Kato, K

    Acta Horticulture   588   313-316   2002年1月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)  

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  • カキ '西条'におけるストレス誘導エチレン生合成と果実軟化

    中野 龍平, 播磨 真志, 小倉 恵美, 井上 真輔, 久保 康隆, 稲葉 昭次

    園芸学会雑誌   70 ( 5 )   581 - 585   2001年9月

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    記述言語:英語   出版者・発行元:園藝學會  

    カキ'西条'果実の軟化に対するエチレンの関与を明らかにするとともに, 果実のエチレン生合成に及ぼすCTSD脱渋に相当するCO<SUB>2</SUB>処理と貯蔵中の湿度条件の影響を調査した.'西条'果実を>95%CO<SUB>2</SUB>で16時間処理した後, 温度20℃湿度40∿60%の条件下で貯蔵すると, 収穫後2日(CO<SUB>2</SUB>処理後1日)よりエチレン生成が検出され, 収穫後5日より軟化果実が多発した.1-methylcyclopropene(MCP)によりエチレンの作用を阻害すると, この急激な軟化は完全に抑えられた.CO<SUB>2</SUB>処理を行わずに, 果実を低湿度下(40∿60%)および高湿度下(>95%)で貯蔵すると, 低湿度下で貯蔵した果実では収穫後2日よりエチレン生成の誘導と急激な軟化が観察された.高湿度下で貯蔵した果実ではエチレン生成・軟化発生とも収穫後10日まで抑えられた.一方, CO<SUB>2</SUB>処理果実では, 高湿度下で貯蔵した場合でも収穫後2日よりエチレン生成とそれに伴う急激な軟化がみられた.以上より, '西条'果実の収穫後の軟化には, 水ストレスおよび脱渋処理に伴うCO<SUB>2</SUB>ストレスによって誘導されるエチレンが関与していることが示された.

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  • Involvement of stress-induced ethylene biosynthesis in fruit softening of 'Saijo' persimmon

    R Nakano, S Harima, E Ogura, S Inoue, Y Kubo, A Inaba

    JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE   70 ( 5 )   581 - 585   2001年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC HORTICULTURAL SCI  

    The possible involvement of stress-induced ethylene in softening of 'Saijo' persimmon fruit with or without >95 % CO2 treatment for 16 hr at 25 degreesC, a technique widely used for the removal of astringency (Constant Temperature Short Duration (CTSD) method) was investigated. To confirm the role of ethylene in fruit softening, fruit were treated with 1-methyleyclopropene (MCP), a strong inhibitor of ethylene perception. The softening of treated fruit was inhibited markedly compared with non-treated fruit. Fruits, with or without CO2 treatment, were held in high (>95 % RH) or low humidity (40-60% RH) at 20 degreesC to determine the effects of water and CO2 stresses on postharvest ethylene production and fruit softening. Without CO2 treatment, ethylene production and fruit softening were retarded significantly by high humidity. Whereas, with CO2 treatment, a considerable amount of ethylene was produced and fruit softened, irrespective of humidity. These results indicate that ethylene is involved in fruit softening and that its evolution is stimulated not only by water stress but also by CO2 Stress in 'Saijo' persimmon fruit.

    DOI: 10.2503/jjshs.70.581

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  • Delay of fruit softening in forcing- cultured 'Tonewase' Japanese persimmon by packaging in perforated polyethylene bags

    R. Nakano, S. Harima, Y. Kubo, A. Inaba

    Journal of the Japanese Society for Horticultural Science   70   385 - 392   2001年6月

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    'Tonewase' is an early maturity strain selected from 'Hiratanenashi', an astringent Japanese persimmon cultivar. In some districts of Japan, 'Tonewase' has been grown under forcing-culture conditions, which often results in rapid softening during postharvest distribution. In this study, we elucidated that this softening is caused by water-stress-induced ethylene that can be suppressed by reducing water loss by using perforated polyethylene bags (PPB). With or without removal of astringency by treatment with elevated carbon dioxide (CTSD method), forcing-cultured 'Tonewase' fruit produced significant amounts of ethylene two days after harvest; thereafter, most fruit softened rapidly. This softening was markedly suppressed by treating the fruit with 1-methylcyclopropene (MCP), an inhibitor of ethylene action, which suggests the involvement of ethylene in fruit softening. Packaging 'Tonewase' fruit in PPB ranging from 0.03% to 0.3% of the total film surface area reduced water loss, retarded the commencement of ethylene production and delayed fruit softening. Immature field-grown 'Hiratanenashi' fruit behaved similarly to the forcing-cultured 'Tonewase' fruit with respect to ethylene production, softening and response to MCP treatment and PPB, whereas mature fruit under the same storage conditions produced no ethylene and remained firm during postharvest.

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  • Microsatellite and CAPS markers for ethylene-related genes, 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase genes, and their variation in melon (Cucumis melo L.)

    Y Akashi, S Shiomi, Y Kubo, M Masuda, K Kato

    BREEDING SCIENCE   51 ( 2 )   107 - 112   2001年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPANESE SOC BREEDING  

    To establish DNA markers for ethylene-related genes in melon (Cucumis melo L.), we searched for microsatellite and cleaved amplified polymorphic sequence (CAPS) polymorphisms in two ACC synthase genes (CMe-ACS1, CMe-ACS2) and two ACC oxidase genes (CMe-ACO1, CMe-ACO3), and found two CAPSs and six microsatellite markers. Five markers were detected in two genes, Me-ACS1 and CMe-ACO1, which were expressed during fruit ripening. A highly polymorphic marker was found in CMe-ACS2, where the number of simple sequence repeats (TA) was highly variable among cultivars, ranging from seven to thirty-seven. Insertion/deletion of (T)(n) and (A)(n) were also detected in CMe-ACS2. Specific CAPS and microsatellites for vars. makuwa and conomon, local varieties in East Asia, were detected in four markers, Acs1-c1, Acs2-ms1, Aco1-ms2 and Aco3-ms1, and could be useful markers for phylogenetic analysis in melon.

    DOI: 10.1270/jsbbs.51.107

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  • Regulation of genes encoding ethylene biosynthetic enzymes in peach (Prunus persica L.) fruit by carbon dioxide and 1-methylcyclopropene

    Francis M. Mathooko, Yuki Tsunashima, Willis Z.O. Owino, Yasutaka Kubo, Akitsugu Inaba

    Postharvest Biology and Technology   21 ( 3 )   265 - 281   2001年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We have cloned one member (PP-ACSI) of the 1-aminocyclopropane-1-carboxylate (ACC) synthase and two members (PP-ACO1 and PP-ACO2) of the ACC oxidase gene families in peach (Prunus persica L.) fruit and studied their expression characteristics during fruit ripening and treatment with CO2 and 1-methylcyclopropene (MCP), inhibitors of ethylene action. Northern analysis showed that the abundance of PP-ACS1, PP-ACO1 and PP-ACO2 mRNAs increased with fruit ripening in parallel with increases in ethylene production and activities of ACC synthase and ACC oxidase. The abundance of PP-ACO2 mRNA was much lower than that of PP-ACO1. CO2 and MCP treatment inhibited ethylene production, ACC synthase activity, and accumulation of PP-ACS1 mRNA. Although CO2 had little effect on ACC oxidase activity, it inhibited the accumulation of PP-ACO1 and PP-ACO2 mRNAs to the same levels as MCP. Wound-induced ethylene production, ACC synthase activity, and the abundance of PP-ACS1 mRNA were blocked and stimulated by CO2 and MCP, respectively. CO2 and MCP had no effect on wound-induced ACC oxidase activity but inhibited the accumulation of its mRNA. Wound-induced activities of ACC synthase and ACC oxidase, and abundance of their mRNAs were inhibited and stimulated, respectively, by exogenous ethylene. The translational inhibitor, cycloheximide inhibited wound-induced ethylene biosynthesis but super-induced the accumulation of PP-ACS1 and PP-ACO1 mRNAs, suggesting that their induction is a primary response to the inducer. These results suggest that the expression of PP-ACS1 and PP-ACO1 genes play a key role in the regulation of ethylene biosynthesis in peach fruit during ripening and in response to wounding. The results also indicate that wound-induced PP-ACS1 and PP-ACO1 genes are under negative and positive control, respectively. Further, using MCP we provide evidence indicating that CO2 does not regulate ACC synthase activity and expression of the PP-ACS1 gene in peach fruit during ripening and in response to wounding by antagonizing ethylene action. © 2001 Elsevier Science B.V.

    DOI: 10.1016/S0925-5214(00)00158-7

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  • Structural characterization of ACC synthase genes from melon and cucumber and their promoter activities determined by GUS transient assay. (2001)

    Shiomi, S, E. Ogura, M. Yamamoto, R. Nakamura, Y. Kubo, A. Inaba

    Sci. Rep. Fac. Agric. Okayama Univ.   2001年

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  • Cloning of genes encoding cell wall modofying enzymes and their expression in persimmon fruit.

    Kubo Y, Nakano R, Inaba A

    Acta Hortic.   2000年

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  • Physiological responses of Chinese pear 'Yali' fruit to CO2-enriched and/or O-2-reduced atmospheres 査読

    B Ning, Y Kubo, A Inaba, R Nakamura

    JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE   66 ( 3-4 )   613 - 620   1997年12月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC HORTICULTURAL SCI  

    Respiration and ethylene production rates of Chinese pear 'Yali' fruit (Pyrus ussuriensis Maxim. var. sinensis Kikuchi) stored in CO2-enriched and/or O-2-reduced atmospheres. In addition, several types of polyethylene film packaging were also applied to the long-term storage of 'Yali' fruit.
    1. Oxygen uptake and ethylene production in 'Yali' fruit at 20 degrees C decreased with increasing CO2 concentration up to 40%. Ethylene production under 60% CO2 was markedly inhibited, whereas O-2 uptake was promoted; a physiological disorder in the flesh developed.
    2. Under the same storage condition, oxygen uptake and ethylene production consistently decreased with decreasing O-2 concentration. However, when O-2 was decreased to less than 5%, CO2 output exceeded O-2 uptake, suggesting that anaerobic respiration was occurring.
    3. Respiration was inhibited in fruit kept at 10 or 20 degrees C under 5% CO2 + 3% O-2 during the first 4 days of storage and then increased suddenly thereafter accompanied by the development of a physiological disorder.
    4. The storage life of the fruit packed in a film with soda lime at 10 degrees C, in which O-2 became to about 8%, was prolonged by about a month, as compared to that of those packed in a perforated bag. The gas concentration higher than 5% CO2 and/or lower than 5% O-2 within a plastic bag caused an accumulation of ethanol and the development of disorder in flesh, thus shortening storage life of fruit.
    Our results suggest that 'Yali' fruit is sensitive to both CO2-enriched and O-2-reduced atmospheres, and that 2% CO2 and 8% O-2 are about optimal for its long-term storage.

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  • Effects of high carbon dioxide exposure on ethylene biosynthesis in peach and tomato fruits 査読

    Y Kubo, K Sakota, A Inaba, R Nakamura

    JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE   65 ( 2 )   409 - 415   1996年9月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC HORTICULTURAL SCI  

    Ethylene production, oxygen uptake, the activities of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase in vivo and the contents of ACC and 1-(malonylamino)cyclopropane-1-carboxylic acid (MACC) were determined in peach and tomato fruits held in carbon dioxide-enriched atmosphere. Ethylene production in peaches decreased to a trace level with 60% carbon dioxide and in tomatoes to 50% of the initial level. The ethylene production rates in both fruits reverted to the initial level when the fruits were transferred back to air. Oxygen uptake in both fruits was markedly inhibited during carbon dioxide exposure. In vivo activities of ACC oxidase and ACC synthase in both fruits were also inhibited during carbon dioxide exposure. ACC content in peaches held in carbon dioxide-enriched atmosphere decreased but it increased in tomatoes. The level of MACC in peaches was constant during carbon dioxide treatment, whereas that in tomatoes slightly increased. These results indicate that inhibition in ethylene production by carbon dioxide may be mediated mainly by reduced conversion of ACC to ethylene in tomatoes, whereas in peaches, the inhibition is attributed to both reduced conversion of S-adenosylmethionine to ACC and ACC to ethylene.

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  • Respiration and ethylene production in fruits and vegetables held in carbon dioxide-enriched atmospheres - Effects of temperature and carbon dioxide concentration 査読

    Y Kubo, O Hirata, A Inaba, R Nakamura

    JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE   65 ( 2 )   403 - 408   1996年9月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC HORTICULTURAL SCI  

    The rates of respiration and ethylene production in various fruits and vegetables held in 0.60% CO2 at 25 degrees C or 60% CO2 at 5-25 degrees C were determined by an automated microcomputer system.
    In peaches, apples, tomatoes, and broccoli, dose-dependent decreases of O-2 uptake and C2H4 production were observed during treatment with various concentrations of CO2 at 25 degrees C. Oxygen uptake in bananas was inhibited at 10% CO2 and higher, whereas C2H4 production increased as the ambient CO2 concentration was elevated. CO2 concentration had little or no effect on O-2 uptake in satsuma mandarin. Oxygen uptake in lettuce at 20% CO2 and below was similar to that under air, whereas induction of C2H4 production and an enhanced O-2 uptake were observed in lettuce held in 40% CO2 and higher.
    Inhibition of O-2 uptake and C2H4 production in peaches by 60% CO2 declined as the temperature was lowered to the range of 5-25 degrees C. In broccoli held in 60% CO2, the inhibition of O-2 uptake was temperature-dependent, but C2H4 production was suppressed to trace level at all temperatures. The induction of C2H4 production and enhancement of O-2 uptake in lettuce by 60% CO2 occurred distinctly at 25 degrees C, slightly at 15 degrees C, but not at 10 degrees C and 5 degrees C.

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  • Softening of several fruits and vegetables at low humidity with respect to their endogenous ethylene concentrations 査読

    YB Xue, K Ishikawa, Y Kubo, A Inaba, R Nakamura

    JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE   65 ( 1 )   169 - 176   1996年6月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC HORTICULTURAL SCI  

    Induction of softening in banana, cucumber, carrot and Japanese radish at low relative humidity (55% RH) was investigated with respect to their endogenous ethylene production. In banana, exogenous ethylene pretreatment rapidly hastened yellowing of the peel and softening of the flesh at both high and low RH. However, pretreatment with diazocyclopentadiene (DACP), an inhibitor of ethylene action, inhibited yellowing but did not prevent softening at high and low RH. In cucumber, carrot, and Japanese radish, softening of the firmness progressed at low RH even though endogenous ethylene activity was inhibited by DACP pretreatment. In cucumber, the flesh softened rapidly at low RH which was positively correlated to the increase in water soluble pectin and a decrease in sodium hexametaphosphate soluble pectin. These results may indicate that induction of softening in several types of fruits and vegetables proceeds independent of endogenous ethylene concentration, and that the degradation of cell wall polysaccharides is induced directly by water deficit stress.

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  • Effects of humidity on physiological activity and texture of tomato and cucumber fruits 査読

    YB Xue, Y Kubo, A Inaba, R Nakamura

    JOURNAL OF THE JAPANESE SOCIETY FOR FOOD SCIENCE AND TECHNOLOGY-NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI   43 ( 2 )   164 - 171   1996年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC FOOD SCIENCE TECHNOLOGY  

    Physiological responses and texture changes were investigated on tomato and cucumber fruits held in high (95%), medium (80%) and low (60%) relative humidity at 20 degrees C. High respiration rates and elevated ethylene productions were observed in both the tomato and the cucumber fruits at low RH. Low RH hastened the peel coloring of the tomatoes in terms of the Hunter L*, a* and b* values. Loss in flesh firmness, which was rapid in the fruit held in low RH, was positively correlated to increased water-soluble pectin (WSP), decreased hydrochloric acid-soluble pectin (HP) and decreased hemicellulose in the tomatoes, and to decreased sodium hexametaphosphate-soluble pectin (HMP) in the cucumbers. These results suggested that water deficit stress in low RH was unfavorable in the view of keeping quality of the tomatoes and the cucumbers, because of accelerating their physiological activities and degrading enzyme activities of cell wall polysaccharides.

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  • Effects of humidity on ripening and texture in banana fruit 査読

    Y Xue, Y Kubo, A Inaba, R Nakamura

    JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE   64 ( 3 )   657 - 664   1995年12月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC HORTICULTURAL SCI  

    Ripening characteristics and texture changes were investigated in green banana (Musa AAA group, Cavendish subgroup cv. Giant Cavendish) fruit held in low (65%) and high (95%) relative humidity at 20 degrees C. Low RK hastened the yellowing of the peel in terms of the Hunter a* and b* values and advanced the onset of the respiratory climacteric rise and ethylene emanation by about 6 and 12 days than high RH, respectively. Loss in flesh firmness, which was rapid in fruit held in low RH, was positively correlated to increased polygalacturonase (PG) activity, increased water-soluble pectin (WSP), and decreased hydrochloric acid-soluble pectin (HP). The appearance of PG activity was 6 and 9 days earlier than ethylene production at high and low RH, respectively, suggesting that induction of cell wall degrading enzyme activity might occur without ethylene emanation. There was no relationship between cellulase activity and softening.
    These results suggest that RH in the storage atmosphere affects weight loss of banana fruit and ultimately influences the ripening characteristics of the fruit.

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  • EFFECTS OF STORAGE-TEMPERATURE ON THE OCCURRENCE OF CHILLING INJURY AND STORAGE LIFE IN CHINESE PEAR YALI 査読

    B NING, Y KUBO, A INABA, R NAKAMURA

    JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE   61 ( 2 )   461 - 467   1992年9月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC HORTICULTURAL SCI  

    To establish a physiological basis for storage and transport of the Chinese pear 'Yali', (Pyrus ussuriensis Maxim. var. sinensis Kikuchi), the occurrence of chilling injury and storage life of fruit stored at 0-degrees, 5-degrees, 10-degrees, and 20-degrees-C were investigated. Inhibitory effects of temperature-conditioning prior to low temperature storage on chilling injury were also studied; the mechanism of conditioning-effect is discussed.
    1. Browning and water-soaked appearance of flesh, which seemed to be symptoms of chilling injury, were observed in the fruit stored at 0-degrees or 5-degrees-C, but not at 10-degrees and 20-degrees-C. After an extended storage, browning of the core occurred at all temperatures, indicating to be a different symptom from that of chilling injury.
    2. The rates of respiration and ethylene production in the fruit decreased with the lowering of storage temperature. The rates of respiration in the fruit transferred to 20-degrees from 5-degrees-C recovered at the level of the fruit stored throughout the experiment at 20-degrees-C. However, respiration rates of fruit injured by the low temperature treatment and then transferred to 20-degrees-C were higher than that of the control.
    3. The electrolyte leakage from flesh discs increased with the occurrence of chilling injury and continued to increase with the development of the injury symptoms.
    4. The occurrence of chilling injury in fruits stored at 5-degrees-C was inhibited by various temperature-conditioning lasting more than three weeks. As stepwise cooling and delayed storage both inhibited the occurrence of chilling injury, the mechanisms of conditioning effects seem to depend on aging rather than on cold-acclimatization of the fruit.
    5. The fruit stored at 5-degrees-C after an appropriate temperature-conditioning retained good eating quality even after 5 months of storage.

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  • INDUCTION OF ETHYLENE PRODUCTION BY RAPE SEED OIL AND ETHANOL IN FIG AND PERSIMMON FRUITS 査読

    JP GAO, Y KUBO, R NAKAMURA, A INABA

    JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE   60 ( 3 )   711 - 717   1991年12月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC HORTICULTURAL SCI  

    The mechanism which induces the ethylene evolution in the fig 'Masui-Dauphin' and Japanese persimmon 'Hiratanenashi' was investigated by treating the fruits with rape seed oil and ethanol.
    The rape seed oil, which was injected through ostiolum of the fig fruit harvested at preclimacteric stage, induced a rapid ethylene evolution, similar to that obtained by treatment with exogenous ethylene. In oil-treated fruits, the activity of ethylene forming enzyme (EFE) increased markedly, attaining a level about 12 times that of control after 24 hr, but 1-aminocyclopropane-1-carboxylic acid (ACC) level increased gradually. The application of 2,5-norbornadiene, a competitive inhibitor of ethylene action, inhibited both EFE activity and ACC accumulation, which led to a significant suppression of ethylene production within 48 hr of the oil treatment. Ethylene production induced by oil was almost competitively inhibited by aminooxyacetic acid (AOA), an inhibitor of ACC synthase. The ethylene production by persimmon fruit slices was also induced by infiltrating oil into the tissue, but this treatment was less effective as compared to responses observed in the fig.
    Ethanol vapors induced rapid ethylene evolution in the fig and persimmon fruits which were harvested at a preclimacteric stage. In the fig, EFE activity increased to a maximum level with vapor concentrations of 0.5 and 1.0 ml ethanol per 100 g fresh fruit weight, but the activity decreased with continuous increases in ethanol concentration. The flesh ACC content, however, tended to increase with much higher ethanol concentration. Ethylene production induced with ethanol was almost competitively prevented by AOA.
    These data suggest that the inducing effect of oil and ethanol on ethylene production in the fig and persimmon fruits is likely the result of a biochemical stress which activates the ACC-ethylene biosynthesis system.

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  • INDUCTION OF ETHYLENE BIOSYNTHESIS IN BANANA FRUIT UNDER DIFFERENT RIPENING CONDITIONS 査読

    JP GAO, Y KUBO, R NAKAMURA, A INABA

    JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE   59 ( 3 )   665 - 671   1990年12月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC HORTICULTURAL SCI  

    In an attempt to understand the mechanism of ethylene biosynthesis induced suddenly at the onset of ripening, banana [Musa (AAA group, Cavendish subgroup) cv. Giant Cavendish] fruit were ripened under these conditions: a) hypobaric pressure, b) three temperature regimes, c) treatment with 2,5-norbornadiene (NBD), d) treatment with manganese (Mn) and e) exogenous ethylene. Changes in the rate of ethylene production, contents of 1-aminocyclopropane-1-carboxylic acid (ACC), and 1-(malonylamino)cyclopropane-1-carboxylic acid (MACC), and the activity of ethylene forming enzyme (EFE) were measured during different fruit ripening stages from preclimacteric to early climacteric rise in respiration.
    About 0.20 nl/g.hr of ethylene and 0.25 nmol/g ACC were detected in immature banana in all the treatments during the preclimacteric period. Their levels remained nearly constant until the onset of ripening. Both ethylene production rate and ACC level was increased markedly with the onset of ripening, while MACC level was increased slightly as the fruits ripened. In contrast, EFE activity increased linearly during the preclimacteric period and reached a peak in all treatments shortly before the onset of ripening. When NBD, a competitive inhibitor of ethylene action, and Mn, and ethylene adsorber, were applied to preclimacteric fruit, the process of increase of EFE activity was prolonged and the number of days until the onset of ripening were extended, but the maximum value of the EFE activity was unaffected, being similar to that of untreated fruit. In fruits treated with ethylene, EFE activity increased rapidly, reaching to the MTT (minimum ethylene exposure time needed to induce ripening); the activity decreased after 24 hr to the previous level in spite of continued treatment.
    These data suggest that a trace amount of internal ethylene existing in banana fruit during the preclimacteric stage may be a causal factor to the elevation of EFE activity as bananas begin to ripen.

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  • EFFECT OF EXOGENOUS ETHYLENE ON RESPIRATION IN FRUITS AND VEGETABLES WITH SPECIAL REFERENCE TO TEMPERATURE 査読

    A INABA, Y KUBO, R NAKAMURA

    JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE   58 ( 3 )   713 - 718   1989年12月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC HORTICULTURAL SCI  

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▼全件表示

書籍等出版物

  • 果実の事典

    朝倉書店  2008年 

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  • 園芸作物保蔵論

    建はく社  2007年  ( ISBN:9784767961

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  • 園芸生理学

    文永堂出版  2007年 

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  • 園芸生理学

    文永堂出版  2007年 

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  • 園芸作物保蔵論

    建はく社  2007年 

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  • 園芸学用語集・作物名編

    養賢堂  2005年 

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  • Biology and Biothechnology of the Plant Hormone Ethylene

    ISO press  2003年 

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  • 最新果樹園芸学

    朝倉書店  2002年 

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  • ファイテク How to みる・きく・はかる-植物環境計測-

    養賢堂  2002年 

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▼全件表示

MISC

  • モモ黒斑病罹病性に関連する遺伝子座領域の推定

    浅野貴洋, 河井崇, 鵜木悠治郎, 日原誠介, 高田大輔, 白澤健太, 赤木剛士, 福田文夫, 中野龍平, 久保康隆, 山本幹博, 牛島幸一郎

    育種学研究   22   2020年

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  • モモ遺伝資源および交雑集団におけるモモ黒斑病の罹病性調査

    浅野貴洋, 河井崇, 伊藤那月, 芦田祐里, 飯島彩加, 鵜木悠治郎, 高田大輔, 中野龍平, 福田文夫, 久保康隆, 赤木剛士, 山本幹博, 牛島幸一郎

    園芸学研究 別冊   19 ( 1 )   2020年

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  • キウイ幼果抽出物は脂肪細胞分化と脂肪細胞へのグルコース取り込みを促進する

    齋藤武, 阿部大吾, 久保康隆, 中村宜督

    近畿中国四国農業研究成果情報(CD-ROM)   2010   2011年

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  • キウイフルーツ幼果抽出物が脂肪細胞分化とアディポサイトカイン発現に及ぼす影響

    阿部大吾, 齋藤武, 久保康隆, 関谷敬三

    日本農芸化学会大会講演要旨集   2009   234   2009年3月

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    記述言語:日本語  

    J-GLOBAL

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  • 環状はく皮処理および反射マルチ敷設がカキ'刀根早生'果実の収穫後の軟化発生に及ぼす影響.

    播磨真志, 中野龍平, 稲葉昭次, 久保康隆

    園芸学研究   2006年

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  • Ethylene biosynthesis in detached young persimmon fruit is initiated in calyx and modulated by water loss from the fruit

    R Nakano, E Ogura, Y Kubo, A Inaba

    PLANT PHYSIOLOGY   131 ( 1 )   276 - 286   2003年1月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Persimmon (Diospyros kaki Thunb.) fruit are usually classified as climacteric fruit; however, unlike typical climacteric fruits, persimmon fruit exhibit a unique characteristic in that the younger the stage of fruit detached, the greater the level of ethylene produced. To investigate ethylene induction mechanisms in detached young persimmon fruit, we cloned three cDNAs encoding 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (DK-ACS1, 2, and -3) and two encoding ACC oxidase (DK-ACO1 and -2) genes involved in ethylene biosynthesis, and we analyzed their expression in various fruit tissues. Ethylene production was induced within a few days of detachment in all fruit tissues tested, accompanied by temporally and spatially coordinated expression of all the DK-ACS and DK-ACO genes. In all tissues except the calyx, treatment with 1-methylcyclopropene, an inhibitor of ethylene action, suppressed ethylene production and ethylene biosynthesis-related gene expression. In the calyx, one ACC synthase gene (DK-ACS2) exhibited increased mRNA accumulation accompanied by a large quantity of ethylene production, and treatment of the fruit with 1-methylcyclopropene did not prevent either the accumulation of DK-ACS2 transcripts or ethylene induction. Furthermore, the alleviation of water loss from the fruit significantly delayed the onset of ethylene production and the expression of DK-ACS2 in the calyx. These results indicate that ethylene biosynthesis in detached young persimmon fruit is initially induced in calyx and is modulated by water loss through transcriptional activation of DK-ACS2. The ethylene produced in the calyx subsequently diffuses to other fruit tissues and acts as a secondary signal that stimulates autocatalytic ethylene biosynthesis in these tissues, leading to a burst of ethylene production.

    DOI: 10.1104/pp.010462

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  • Ethylene biosynthesis in detached young persimmon fruit is initiated in calyx and modulated by water loss from the fruit

    R Nakano, E Ogura, Y Kubo, A Inaba

    PLANT PHYSIOLOGY   131 ( 1 )   276 - 286   2003年1月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    Persimmon (Diospyros kaki Thunb.) fruit are usually classified as climacteric fruit; however, unlike typical climacteric fruits, persimmon fruit exhibit a unique characteristic in that the younger the stage of fruit detached, the greater the level of ethylene produced. To investigate ethylene induction mechanisms in detached young persimmon fruit, we cloned three cDNAs encoding 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (DK-ACS1, 2, and -3) and two encoding ACC oxidase (DK-ACO1 and -2) genes involved in ethylene biosynthesis, and we analyzed their expression in various fruit tissues. Ethylene production was induced within a few days of detachment in all fruit tissues tested, accompanied by temporally and spatially coordinated expression of all the DK-ACS and DK-ACO genes. In all tissues except the calyx, treatment with 1-methylcyclopropene, an inhibitor of ethylene action, suppressed ethylene production and ethylene biosynthesis-related gene expression. In the calyx, one ACC synthase gene (DK-ACS2) exhibited increased mRNA accumulation accompanied by a large quantity of ethylene production, and treatment of the fruit with 1-methylcyclopropene did not prevent either the accumulation of DK-ACS2 transcripts or ethylene induction. Furthermore, the alleviation of water loss from the fruit significantly delayed the onset of ethylene production and the expression of DK-ACS2 in the calyx. These results indicate that ethylene biosynthesis in detached young persimmon fruit is initially induced in calyx and is modulated by water loss through transcriptional activation of DK-ACS2. The ethylene produced in the calyx subsequently diffuses to other fruit tissues and acts as a secondary signal that stimulates autocatalytic ethylene biosynthesis in these tissues, leading to a burst of ethylene production.

    DOI: 10.1104/pp.010462

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  • 有孔および無孔ポリエチレン包装によるハウス栽培‘刀根早生’果実の軟化抑制技術の確立.

    播磨真志, 中野龍平, 山内 勧, 北野欣信, 久保康隆, 稲葉昭次, 富田栄一

    園芸学会雑誌   2002年

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  • カキ‘刀根早生’促成栽培果実の出荷容器の改善による軟化抑制.

    播磨真志, 中野龍平, 山内 勧, 北野欣信, 久保康隆, 稲葉昭次, 富田栄一

    園芸学会雑誌   2002年

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  • 有孔ポリエチレン包装によるカキ‘刀根早生’ハウス栽培果実の軟化抑制

    中野龍平, 播磨真志, 久保康隆, 稲葉昭次

    園芸学会雑誌   70(3):385-392   2001年

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  • 種々の保存温度下における有孔ポリエチレン包装によるハウス栽培カキ‘刀根早生’果実の軟化抑制.

    播磨真志, 中野龍平, 山内 勧, 北野欣信, 久保康隆, 稲葉昭次, 富田栄一

    日本食品保蔵科学会誌   2001年

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  • 有孔ポリエチレン包装によるカキ‘刀根早生’ハウス栽培果実の軟化抑制

    中野龍平, 播磨真志, 久保康隆, 稲葉昭次

    園芸学会雑誌   2001年

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  • カキ‘刀根早生’促成栽培果実の収穫後の軟化発生

    播磨真志, 中野龍平, 山本貴司, 小松英雄, 藤本欣司, 北野欣信, 久保康隆, 稲葉昭次, 富田栄一

    園芸学会雑誌   2001年

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  • Extraction and properties of 1-aminocyclopropane-1-carboxylate synthase in banana fruit

    XJ Liu, R Nakano, Y Kubo, A Inaba

    JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE   69 ( 6 )   696 - 701   2000年11月

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    記述言語:英語   出版者・発行元:JAPAN SOC HORTICULTURAL SCI  

    The determination of 1-aminocyclopropane-1-carboxylate (ACC) synthase activity is essential in understanding its role in the ethylene biosynthesis during fruit ripening. Because of the high level of soluble tannins, to date, there has been no report on successful determination of banana ACC synthase activity. In this study, we examined the method of Badran and Jones (1965) for the extraction of ACC synthase from banana fruit. The extraction procedure consists of homogenizing the pulp in polyethyleneglycol (PEG)-added buffer, and then washing the homogenate with acetone. This PEG-acetone method gave a high ACC synthase activity. The common extraction method using polyvinylpyrrolidone (PVP) yielded a much lower or no ACC synthase activity. The changes in ACC synthase activity determined by the PEG- acetone method correlated with changes in ethylene production during ripening. The optimum pH, Km value for substrate S- adenosylmethionine (SAM), and half-life in the presence of SAM for banana ACC synthase were 9.0, 88 muM, and 18 min respectively. These values were within the range previously reported for ACC synthase in various plant tissues. From these results, we recommend the following extraction method to determine banana ACC synthase activity: freeze the flesh tissue in liquid nitrogen, store it at -80 degreesC until use, homogenize the frozen sample in a Waring blender with PEG- added extraction buffer, and later precipitate the enzyme mixture with acetone.

    DOI: 10.2503/jjshs.69.696

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  • Expression of a water stress-induced polygalacturonase gene in harvested cucumber fruit

    Y Kubo, YB Xue, A Nakatsuka, FM Mathooko, A Inaba, R Nakamura

    JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE   69 ( 3 )   273 - 279   2000年5月

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    記述言語:英語   出版者・発行元:JAPAN SOC HORTICULTURAL SCI  

    We investigated the molecular response of harvested cucumber (Cucumis sativus L.) fruit to postharvest water stress. Using RT-PCR, we isolated CUPG1 cDNA, a cDNA clone encoding polygalacturonase (PG) that is inducible by water stress in cucumber fruit and characterized the expression of the gene with respect to hormonal control. The full length sequence determined by RACE-PCR, encoded a deduced protein of 435 amino acids that contained several common conserved domains as reported for PGs. The CUPG1 gene expression was also induced by exogenous ethylene but not by the application of abscisic acid (ABA). The level of CUPG1 mRNA induced by water stress, was reduced by 50% in fruits pre-treated with 1-methyl-cyclopropene (MCP), a strong inhibitor of ethylene action, which suggests that the expression of CUPG1 gene could be induced by a direct mechanism through water stress and an indirect mechanism mediated through ethylene produced in response to water stress. We conclude that water loss in harvested cucumber fruit results not only in physical changes but also leads to molecular responses, including PG gene expression.

    DOI: 10.2503/jjshs.69.273

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  • Expression of a water stress-induced polygalacturonase gene in harvested cucumber fruit

    Yasutaka Kubo, Yanbin Xue, Akira Nakatsuka, Francis M. Mathooko, Akitsugu Inaba, Reinosuke Nakamura

    Journal of Japanese Society for Horticultural Science   69 ( 3 )   273 - 279   2000年5月

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    We investigated the molecular response of harvested cucumber (Cucumis sativus L.) fruit to postharvest water stress. Using RT-PCR, we isolated CUPG1 cDNA, a cDNA clone encoding polygalacturonase (PG) that is inducible by water stress in cucumber fruit and characterized the expression of the gene with respect to hormonal control. The full length sequence determined by RACE-PCR, encoded a deduced protein of 435 amino acids that contained several common conserved domains as reported for PGs. The CUPG1 gene expression was also induced by exogenous ethylene but not by the application of abscisic acid (ABA). The level of CUPG1 mRNA induced by water stress, was reduced by 50% in fruits pre-treated with 1-methylcyclopropene (MCP), a strong inhibitor of ethylene action, which suggests that the expression of CUPG1 gene could be induced by a direct mechanism through water stress and an indirect mechanism mediated through ethylene produced in response to water stress. We conclude that water loss in harvested cucumber fruit results not only in physical changes but also leads to molecular responses, including PG gene expression.

    DOI: 10.2503/jjshs.69.273

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  • Expression of a water stress-induced polygalacturonase gene in harvested cucumber fruit

    Journal of Japanese Society for Horticultural Science   69 ( 3 )   273 - 279   2000年5月

  • 高濃度炭酸ガス環境と青果物の鮮度保持. 55:140-143

    久保康隆

    農耕と園芸   55:140-143   2000年

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  • Characterization of ethylene biosynthesis associated with ripening in banana fruit

    Xuejun Liu, Shinjiro Shiomi, Akira Nakatsuka, Yasutaka Kubo, Reinosuke Nakamura, Akitsugu Inaba

    Plant Physiology   121 ( 4 )   1257 - 1265   1999年

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    記述言語:英語   出版者・発行元:American Society of Plant Biologists  

    We investigated the characteristics of ethylene biosynthesis associated with ripening in banana (Musa sp. [AAA group, Cavendish subgroup] cv Grand Nain) fruit. MA-ACS1 encoding 1-aminocyclopropane-1-carboxylic acid (ACC) synthase in banana fruit was the gene related to the ripening process and was inducible by exogenous ethylene. At the onset of the climacteric period in naturally ripened fruit, ethylene production increased greatly, with a sharp peak concomitant with an increase in the accumulation of MA-ACS1 mRNA, and then decreased rapidly. At the onset of ripening, the in vivo ACC oxidase activity was enhanced greatly, followed by an immediate and rapid decrease. Expression of the MA-ACO1 gene encoding banana ACC oxidase was detectable at the preclimacteric stage, increased when ripening commenced, and then remained high throughout the later ripening stage despite of a rapid reduction in the ACC oxidase activity. This discrepancy between enzyme activity and gene expression of ACC oxidase could be, at least in part, due to reduced contents of ascorbate and iron, cofactors for the enzyme, during ripening. Addition of these cofactors to the incubation medium greatly stimulated the in vivo ACC oxidase activity during late ripening stages. The results suggest that ethylene production in banana fruit is regulated by transcription of MA-ACS1 until climacteric rise and by reduction of ACC oxidase activity possibly through limited in situ availability of its cofactors once ripening has commenced, which in turn characterizes the sharp peak of ethylene production.

    DOI: 10.1104/pp.121.4.1257

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  • Characterization of ethylene biosynthesis associated with ripening in banana fruit

    Xuejun Liu, Shinjiro Shiomi, Akira Nakatsuka, Yasutaka Kubo, Reinosuke Nakamura, Akitsugu Inaba

    Plant Physiology   121 ( 4 )   1257 - 1265   1999年

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    記述言語:英語   出版者・発行元:American Society of Plant Biologists  

    We investigated the characteristics of ethylene biosynthesis associated with ripening in banana (Musa sp. [AAA group, Cavendish subgroup] cv Grand Nain) fruit. MA-ACS1 encoding 1-aminocyclopropane-1-carboxylic acid (ACC) synthase in banana fruit was the gene related to the ripening process and was inducible by exogenous ethylene. At the onset of the climacteric period in naturally ripened fruit, ethylene production increased greatly, with a sharp peak concomitant with an increase in the accumulation of MA-ACS1 mRNA, and then decreased rapidly. At the onset of ripening, the in vivo ACC oxidase activity was enhanced greatly, followed by an immediate and rapid decrease. Expression of the MA-ACO1 gene encoding banana ACC oxidase was detectable at the preclimacteric stage, increased when ripening commenced, and then remained high throughout the later ripening stage despite of a rapid reduction in the ACC oxidase activity. This discrepancy between enzyme activity and gene expression of ACC oxidase could be, at least in part, due to reduced contents of ascorbate and iron, cofactors for the enzyme, during ripening. Addition of these cofactors to the incubation medium greatly stimulated the in vivo ACC oxidase activity during late ripening stages. The results suggest that ethylene production in banana fruit is regulated by transcription of MA-ACS1 until climacteric rise and by reduction of ACC oxidase activity possibly through limited in situ availability of its cofactors once ripening has commenced, which in turn characterizes the sharp peak of ethylene production.

    DOI: 10.1104/pp.121.4.1257

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  • Expression characteristics of CS-ACS1, CS-ACS2 and CS-ACS3, three members of the 1-aminocyclopropane-1-carboxylate synthase gene family in cucumber (Cucumis sativus L.) fruit under carbon dioxide stress

    Francis M. Mathooko, Mercy W. Mwaniki, Akira Nakatsuka, Shinjiro Shiomi, Yasutaka Kubo, Akitsugu Inaba, Reinosuke Nakamura

    Plant and Cell Physiology   40 ( 2 )   164 - 172   1999年

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    記述言語:英語   出版者・発行元:Japanese Society of Plant Physiologists  

    We investigated the expression pattern of three 1-aminocyclopropane-1-carboxylate (ACC) synthase genes, CS-ACS1, CS-ACS2 and CS-ACS3 in cucumber (Cucumis sativus L.) fruit under CO2 stress. CO2 stress-induced ethylene production paralleled the accumulation of only CSACS1 transcripts which disappeared upon withdrawal of CO2. Cycloheximide inhibited the CO2 stress-induced ethylene production but superinduced the accumulation of CS-ACS1 transcript. At higher concentrations, cycloheximide also induced the accumulation of CS-ACS2 and CS-ACS3 transcripts. In the presence of CO2 and cycloheximide, the accumulation of CS-ACS2 transcript occurred within 1 h, disappeared after 3 h and increased greatly upon withdrawal of CO2. Inhibitors of protein kinase and types 1 and 2A protein phosphatases which inhibited and stimulated, respectively, CO2 stress-induced ethylene production had little effect on the expression of these genes. The results presented here identify CS-ACS1 as the main ACC synthase gene responsible for the increased ethylene biosynthesis in cucumber fruit under CO2 stress and suggest that this gene is a primary response gene and its expression is under negative control since it is expressed by treatment with cycloheximide. The results further suggest that the regulation of CO2 stress-induced ethylene biosynthesis by reversible protein phosphorylation does not result from enhanced ACC synthase transcription.

    DOI: 10.1093/oxfordjournals.pcp.a029524

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  • Expression characteristics of CS-ACS1, CS-ACS2 and CS-ACS3, three members of the 1-aminocyclopropane-1-carboxylate synthase gene family in cucumber (Cucumis sativus L.) fruit under carbon dioxide stress

    Francis M. Mathooko, Mercy W. Mwaniki, Akira Nakatsuka, Shinjiro Shiomi, Yasutaka Kubo, Akitsugu Inaba, Reinosuke Nakamura

    Plant and Cell Physiology   40 ( 2 )   164 - 172   1999年

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    記述言語:英語   出版者・発行元:Japanese Society of Plant Physiologists  

    We investigated the expression pattern of three 1-aminocyclopropane-1-carboxylate (ACC) synthase genes, CS-ACS1, CS-ACS2 and CS-ACS3 in cucumber (Cucumis sativus L.) fruit under CO2 stress. CO2 stress-induced ethylene production paralleled the accumulation of only CSACS1 transcripts which disappeared upon withdrawal of CO2. Cycloheximide inhibited the CO2 stress-induced ethylene production but superinduced the accumulation of CS-ACS1 transcript. At higher concentrations, cycloheximide also induced the accumulation of CS-ACS2 and CS-ACS3 transcripts. In the presence of CO2 and cycloheximide, the accumulation of CS-ACS2 transcript occurred within 1 h, disappeared after 3 h and increased greatly upon withdrawal of CO2. Inhibitors of protein kinase and types 1 and 2A protein phosphatases which inhibited and stimulated, respectively, CO2 stress-induced ethylene production had little effect on the expression of these genes. The results presented here identify CS-ACS1 as the main ACC synthase gene responsible for the increased ethylene biosynthesis in cucumber fruit under CO2 stress and suggest that this gene is a primary response gene and its expression is under negative control since it is expressed by treatment with cycloheximide. The results further suggest that the regulation of CO2 stress-induced ethylene biosynthesis by reversible protein phosphorylation does not result from enhanced ACC synthase transcription.

    DOI: 10.1093/oxfordjournals.pcp.a029524

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  • チュウゴクナシ果実におけるエチレン生合成関連遺伝子発現の品種間比較

    阿部大吾, 中野龍平, 久保康隆, 稲葉昭次

    園芸学会雑誌 別冊   68 ( 2 )   1999年

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  • cDNA cloning of ACC synthase and ACC oxidase genes in cucumber fruit and their differential expression by wounding and auxin

    Shinjiro Shiomi, Mikihiro Yamamoto, Teiji Ono, Katsuya Kakiuchi, Junichi Nakamoto, Akira Nakatsuka, Yasutaka Kubo, Reinosuke Nakamura, Akitsugu Inaba, Hidemasa Imaseki

    Journal of Japanese Society for Horticultural Science   67 ( 5 )   685 - 692   1998年9月

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    We cloned and sequenced three cDNA homologs of 1-aminocyclopropane-1-carboxylate (ACC) synthase (pCS-ACS1, pCS-ACS2 and pCS-ACS3) and two of ACC oxidase (pCS-ACO1 and pCS-ACO2) from cucumber (Cucumis sativus L.) fruit using RT-PCR (reverse transcription and polymerase chain reaction) and RACE (rapid amplification of cDNA ends) PCR. Conserved amino acid sequences reported for other plant tissues were found in all the cloned ACC synthase and ACC oxidase cDNAs. Among the ACC synthase and ACC oxidase cDNA clones, pCS-ACS1, pCS-ACS3, and pCS-ACO1 were highly homologous to ACC synthase and ACC oxidase genes cloned from other Cucurbitaceae family at both the nucleotide and the amino acid levels. Northern blot analysis showed that wounding induced the accumulation of CS-ACS1, CS-ACS2, and CS-ACO1 mRNAs, whereas IAA treatment induced expression of all the three ACC synthase genes and CS-ACO1. These expressions paralleled ethylene production in both treatments. Transcripts for CS-ACO2 were constitutively expressed and were not affected by wounding and IAA treatments. From the expression pattern and the sequence similarities of these genes, we conclude that CS-ACS1 could be a wound-inducible gene and that CS-ACS3 is an auxin-inducible one. These results strongly suggest that in cucumber fruit these genes are differentially expressed by wounding and auxin and are involved in the regulation of ethylene biosynthesis at the transcriptional level.

    DOI: 10.2503/jjshs.67.685

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  • cDNA cloning of ACC synthase and ACC oxidase genes in cucumber fruit and their differential expression by wounding and auxin

    Journal of Japanese Society for Horticultural Science   67 ( 5 )   685 - 692   1998年9月

  • Differential expression and internal feedback regulation of 1-aminocyclopropane-1-carboxylate synthase, l-aminocyclopropane-1-carboxylate oxidase, and ethylene receptor genes in tomato fruit during development and ripening

    Akira Nakatsuka, Shiho Murachi, Hironori Okunishi, Shinjiro Shiomi, Ryohei Nakano, Yasutaka Kubo, Akitsugu Inaba

    Plant Physiology   118 ( 4 )   1295 - 1305   1998年

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    記述言語:英語   出版者・発行元:American Society of Plant Biologists  

    We investigated the feedback regulation of ethylene biosynthesis in tomato (Lycopersicon esculentum) fruit with respect to the transition from system 1 to system 2 ethylene production. The abundance of LE-ACS2, LE-ACS4, and NR mRNAs increased in the ripening fruit concomitant with a burst in ethylene production. These increases in mRNAs with ripening were prevented to a large extent by treatment with 1-methylcyclopropene (MCP), an ethylene action inhibitor. Transcripts for the LE-ACS6 gene, which accumulated in preclimacteric fruit but not in untreated ripening fruit, did accumulate in ripening fruit treated with MCP. Treatment of young fruit with propylene prevented the accumulation of transcripts for this gene. LE-ACS1A, LE-ACS3, and TAE1 genes were expressed constitutively in the fruit throughout development and ripening irrespective of whether the fruit was treated with MCP or propylene. The transcripts for LE-ACO1 and LE-ACO4 genes already existed in preclimacteric fruit and increased greatly when ripening commenced. These increases in LE-ACO mRNA with ripening were also prevented by treatment with MCP. The results suggest that in tomato fruit the preclimacteric system 1 ethylene is possibly mediated via constitutively expressed LE-ACS1A and LE-ACS3 and negatively feedback-regulated LE-ACS6 genes with preexisting LE-ACO1 and LE-ACO4 mRNAs. At the onset of the climacteric stage, it shifts to system 2 ethylene, with a large accumulation of LE-ACS2, LE-ACS4, LE-ACO1, and LE-ACO4 mRNAs as a result of a positive feedback regulation. This transition from system 1 to system 2 ethylene production might be related to the accumulated level of NR mRNA.

    DOI: 10.1104/pp.118.4.1295

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  • Differential expression and internal feedback regulation of 1-aminocyclopropane-1-carboxylate synthase, l-aminocyclopropane-1-carboxylate oxidase, and ethylene receptor genes in tomato fruit during development and ripening

    Akira Nakatsuka, Shiho Murachi, Hironori Okunishi, Shinjiro Shiomi, Ryohei Nakano, Yasutaka Kubo, Akitsugu Inaba

    Plant Physiology   118 ( 4 )   1295 - 1305   1998年

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    記述言語:英語   出版者・発行元:American Society of Plant Biologists  

    We investigated the feedback regulation of ethylene biosynthesis in tomato (Lycopersicon esculentum) fruit with respect to the transition from system 1 to system 2 ethylene production. The abundance of LE-ACS2, LE-ACS4, and NR mRNAs increased in the ripening fruit concomitant with a burst in ethylene production. These increases in mRNAs with ripening were prevented to a large extent by treatment with 1-methylcyclopropene (MCP), an ethylene action inhibitor. Transcripts for the LE-ACS6 gene, which accumulated in preclimacteric fruit but not in untreated ripening fruit, did accumulate in ripening fruit treated with MCP. Treatment of young fruit with propylene prevented the accumulation of transcripts for this gene. LE-ACS1A, LE-ACS3, and TAE1 genes were expressed constitutively in the fruit throughout development and ripening irrespective of whether the fruit was treated with MCP or propylene. The transcripts for LE-ACO1 and LE-ACO4 genes already existed in preclimacteric fruit and increased greatly when ripening commenced. These increases in LE-ACO mRNA with ripening were also prevented by treatment with MCP. The results suggest that in tomato fruit the preclimacteric system 1 ethylene is possibly mediated via constitutively expressed LE-ACS1A and LE-ACS3 and negatively feedback-regulated LE-ACS6 genes with preexisting LE-ACO1 and LE-ACO4 mRNAs. At the onset of the climacteric stage, it shifts to system 2 ethylene, with a large accumulation of LE-ACS2, LE-ACS4, LE-ACO1, and LE-ACO4 mRNAs as a result of a positive feedback regulation. This transition from system 1 to system 2 ethylene production might be related to the accumulated level of NR mRNA.

    DOI: 10.1104/pp.118.4.1295

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  • Postharvest ripening and ethylene biosynthesis in purple passion fruit

    Shinjiro Shiomi, Yasutaka Kubo, Leonard S. Wamocho, Hiroshi Koaze, Reinosuke Nakamura, Akitugu Inaba

    Postharvest Biology and Technology   8 ( 3 )   199 - 207   1996年

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    記述言語:英語   出版者・発行元:Elsevier B.V.  

    A study was undertaken to investigate the pattern of changes in some chemical constituents, ethylene biosynthesis, and the effect of ethylene treatment during postharvest ripening in purple passion fruit (Passiflora edulis Sims.). During ripening, sucrose content decreased while fructose and glucose contents increased. Citric and malic acid contents slightly increased during the early stage of ripening and decreased thereafter. Amino acids did not change significantly, except for proline, which increased rapidly towards the late stage of ripening. While 1-aminocyclopropane-1-carboxylic acid (ACC) content and ACC synthase activity increased in parallel with ethylene production, ACC oxidase activity was already high when harvested at the turning stage and further increased during ripening. Application of 1000 ppm ethylene for 24 h did not induce earlier onset of ethylene production when applied on harvest day, but was effective when applied one day or five days after harvest. The results indicate that purple passion fruit produces ethylene with the same biosynthetic pathway as other tissues in higher plants
    its ethylene biosynthesis is regulated mainly by ACC synthase activity, and the sensitivity to ethylene might have changed after harvest as the fruit ripened.

    DOI: 10.1016/0925-5214(95)00073-9

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  • Postharvest ripening and ethylene biosynthesis in purple passion fruit

    Shinjiro Shiomi, Yasutaka Kubo, Leonard S. Wamocho, Hiroshi Koaze, Reinosuke Nakamura, Akitugu Inaba

    Postharvest Biology and Technology   8 ( 3 )   199 - 207   1996年

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    記述言語:英語   出版者・発行元:Elsevier B.V.  

    A study was undertaken to investigate the pattern of changes in some chemical constituents, ethylene biosynthesis, and the effect of ethylene treatment during postharvest ripening in purple passion fruit (Passiflora edulis Sims.). During ripening, sucrose content decreased while fructose and glucose contents increased. Citric and malic acid contents slightly increased during the early stage of ripening and decreased thereafter. Amino acids did not change significantly, except for proline, which increased rapidly towards the late stage of ripening. While 1-aminocyclopropane-1-carboxylic acid (ACC) content and ACC synthase activity increased in parallel with ethylene production, ACC oxidase activity was already high when harvested at the turning stage and further increased during ripening. Application of 1000 ppm ethylene for 24 h did not induce earlier onset of ethylene production when applied on harvest day, but was effective when applied one day or five days after harvest. The results indicate that purple passion fruit produces ethylene with the same biosynthetic pathway as other tissues in higher plants
    its ethylene biosynthesis is regulated mainly by ACC synthase activity, and the sensitivity to ethylene might have changed after harvest as the fruit ripened.

    DOI: 10.1016/0925-5214(95)00073-9

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  • 高濃度炭酸ガスがモモおよびトマト果実のエチレン生合成におよぼす影響.

    久保康隆

    園学雑.   65   409 - 415   1996年

  • Regulation of mitochondrial activity in cucumber fruit, broccoli buds and carrot by carbon dioxide

    FM Mathooko, T Fukuda, Y Kubo, A Inaba, R Nakamura

    POSTHARVEST PHYSIOLOGY OF FRUITS   398 ( 398 )   71 - 79   1995年

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    記述言語:英語   出版者・発行元:INTERNATIONAL SOCIETY HORTICULTURAL SCIENCE  

    Respiration rates based on O-2 consumption by intact commodity and by mitochondria from cucumber fruit (Cucumis sativus L.), broccoli (Brassica oleracea L.) and carrot (Daucus carota L.) held at 25 degrees C in controlled atmospheres containing elevated CO2 levels and 20% O-2 (the balance gas being N-2) were investigated. Respiration rate in intact commodity was measured using an automated system connected to a microcomputer. Elevated CO2 stimulated, inhibited and had little effect on the respiration rates of cucumber fruit, broccoli and carrot, respectively. Based on the oxidation of exogenously applied succinate, NADH and malate, mitochondria from CO2-treated cucumber fruit and broccoli buds oxidized these substrates at higher and lower rates, respectively, compared to rates of mitochondria from the respective control samples. Mitochondria from carrot treated with 60% CO2 oxidized these substrates at rates more or less similar to that of control. The respiratory control ratios of mitochondria from CO2-treated cucumber fruit and broccoli buds were lower and their respective ADP:O ratios were lower and higher, respectively, compared to those of mitochondria from the control samples. Addition of CO2 to the reaction medium inhibited the activities of mitochondria from fresh cucumber fruit, broccoli buds and carrot, and their respective oxidative phosphorylation were less efficient. These results indicate that the regulation of respiration in intact cucumber fruit, broccoli buds and carrot by CO2 is positively correlated to the activities of mitochondria extracted therefrom.

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  • Induction of ethylene biosynthesis and polyamine accumulation in cucumber fruit in response to carbon dioxide stress

    Francis M. Mathooko, Yasutaka Kubo, Akitsugu Inaba, Reinosuke Nakamura

    Postharvest Biology and Technology   5 ( 1-2 )   51 - 65   1995年

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    記述言語:英語  

    Carbon dioxide stress-induced ethylene biosynthesis, respiration and polyamine accumulation in cucumber fruit (Cucumis sativus L. cv. Sharp-1) held at 25 °C was investigated. Control fruit produced little ethylene and the respiration rate decreased with increase in incubation time while polyamine levels decreased. Elevated CO2 induced ethylene production, respiration and polyamine accumulation. Putrescine and spermidine levels increased in response to CO2 treatment, whereas spermine levels were not significantly affected. No cadaverine was detected in all treatments. The increase in ethylene production paralleled increases in 1-aminocyclopropane-1-carboxylic acid (ACC) and the activities of both ACC synthase and in vitro ACC oxidase. Infiltration of the fruit with aminooxyacetic acid, a potent inhibitor of the conversion of S-adenosylmethionine (AdoMet) to ACC completely blocked CO2 stress-induced ethylene production. Similarly, cycloheximide, an inhibitor of nucleocytoplasmic protein synthesis effectively blocked CO2 stress induction of polyamine accumulation, ethylene production, ACC formation and the development of ACC synthase. Withdrawal of CO2 gas caused cessation of increases in ethylene production, respiration, ACC, putrescine and the activities of ACC synthase and ACC oxidase, but caused increase in spermidine and spermine levels. These data indicate that CO2 induces de navo synthesis of ACC synthase thereby causing accumulation of ACC and increase in ethylene production and suggest that the conversion of AdoMet to ACC is the rate-limiting step in CO2 stress-induced ethylene biosynthesis. The induction, however, requires continuous presence of the stimulus. The results also suggest that protein synthesis might be required for the CO2 stress induction of polyamine biosynthesis. The results further suggest that in cucumber fruit under CO2 stress, at least, the ethylene and polyamine biosynthetic pathways are not competitive. © 1995.

    DOI: 10.1016/0925-5214(94)00003-B

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  • Regulation of mitochondrial activity in cucumber fruit, broccoli buds and carrot by carbon dioxide

    FM Mathooko, T Fukuda, Y Kubo, A Inaba, R Nakamura

    POSTHARVEST PHYSIOLOGY OF FRUITS   398 ( 398 )   71 - 79   1995年

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    記述言語:英語   出版者・発行元:INTERNATIONAL SOCIETY HORTICULTURAL SCIENCE  

    Respiration rates based on O-2 consumption by intact commodity and by mitochondria from cucumber fruit (Cucumis sativus L.), broccoli (Brassica oleracea L.) and carrot (Daucus carota L.) held at 25 degrees C in controlled atmospheres containing elevated CO2 levels and 20% O-2 (the balance gas being N-2) were investigated. Respiration rate in intact commodity was measured using an automated system connected to a microcomputer. Elevated CO2 stimulated, inhibited and had little effect on the respiration rates of cucumber fruit, broccoli and carrot, respectively. Based on the oxidation of exogenously applied succinate, NADH and malate, mitochondria from CO2-treated cucumber fruit and broccoli buds oxidized these substrates at higher and lower rates, respectively, compared to rates of mitochondria from the respective control samples. Mitochondria from carrot treated with 60% CO2 oxidized these substrates at rates more or less similar to that of control. The respiratory control ratios of mitochondria from CO2-treated cucumber fruit and broccoli buds were lower and their respective ADP:O ratios were lower and higher, respectively, compared to those of mitochondria from the control samples. Addition of CO2 to the reaction medium inhibited the activities of mitochondria from fresh cucumber fruit, broccoli buds and carrot, and their respective oxidative phosphorylation were less efficient. These results indicate that the regulation of respiration in intact cucumber fruit, broccoli buds and carrot by CO2 is positively correlated to the activities of mitochondria extracted therefrom.

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  • Regulation by carbon dioxide of wound-induced ethylene biosynthesis in tomato pericarp and winter squash mesocarp tissues

    Francis M. Mathooko, Yasutaka Kubo, Akitsugu Inaba, Reinosuke Nakamura

    Postharvest Biology and Technology   3 ( 1 )   27 - 38   1993年

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    記述言語:英語  

    The effects of CO2 treatment on wound-induced ethylene biosynthesis were investigated in excised pericarp and mesocarp tissues of tomato (Lycopersicon esculentum Mill. cv. TVR-II) and winter squash (Cucurbita maxima Duch. cv. Ebisu) respectively. Wounding caused increase in ethylene production rate, the levels of 1-aminocyclopropane-1-carboxylic acid (ACC) and 1-(malonylamino)cyclopropane-1-carboxylic acid (MACC) and the activities of ACC synthase and ACC oxidase. The increase in the rate of ethylene production and ACC synthase activity were suppressed by CO2 treatment and was dependent on CO2 concentration. CO2 treatment also suppressed wound-induced increase in ACC level irrespective of the CO2 concentration. The presence of 10% CO2 had no effect on ACC conjugation to MACC induced by wounding while 60% CO2 suppressed almost completely ACC conjugation. CO2 promoted and inhibited induction of ACC oxidase activity in excised tomato and winter squash tissues respectively. These results suggest that CO2 inhibits wound-induced ethylene biosynthesis by reducing the availability of ACC through repression of ACC synthase synthesis and/or inhibition of its activity. Also endogenous ethylene produced in response to wounding may play a role in the regulation of wound-induced ethylene production. © 1993.

    DOI: 10.1016/0925-5214(93)90024-W

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  • Inhibition of auxin-induced ethylene production in cucumber fruit discs by carbon dioxide

    Francis M. Mathooko, Yasutaka Kubo, Akitsugu Inaba, Reinosuke Nakamura

    Postharvest Biology and Technology   3 ( 4 )   313 - 325   1993年

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    記述言語:英語  

    The induction of ethylene biosynthesis by indole-3-acetic acid (IAA) and its regulation by CO2 was examined in cucumber (Cucumis sativus L., var. Sharp-1) fruit discs. Treatment of discs with IAA significantly stimulated ethylene production and the extent depended on IAA concentration upto 1 mM. The increase in IAA-induced ethylene production was accompanied by increase in the activities of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase (also known as ethylene-forming enzyme) and a massive accumulation of ACC and 1-(malonylamino)cyclopropane-1-carboxylic acid (MACC). The induction of ethylene production by IAA was inhibited by treatment with CO2. The extent of inhibition was dependent upon the concentration of CO2. Carbon dioxide inhibited IAA-induced ethylene biosynthesis by suppressing ACC synthase and ACC oxidase activities. Additionally, CO2 inhibited ACC conjugation into MACC. The results indicate that inhibition of ethylene production by CO2 in cucumber fruit discs following IAA treatment is due to inhibition of both ACC synthesis and ACC oxidation. © 1993.

    DOI: 10.1016/0925-5214(93)90012-R

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  • Regulation by carbon dioxide of wound-induced ethylene biosynthesis in tomato pericarp and winter squash mesocarp tissues

    Francis M. Mathooko, Yasutaka Kubo, Akitsugu Inaba, Reinosuke Nakamura

    Postharvest Biology and Technology   3 ( 1 )   27 - 38   1993年

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    記述言語:英語  

    The effects of CO2 treatment on wound-induced ethylene biosynthesis were investigated in excised pericarp and mesocarp tissues of tomato (Lycopersicon esculentum Mill. cv. TVR-II) and winter squash (Cucurbita maxima Duch. cv. Ebisu) respectively. Wounding caused increase in ethylene production rate, the levels of 1-aminocyclopropane-1-carboxylic acid (ACC) and 1-(malonylamino)cyclopropane-1-carboxylic acid (MACC) and the activities of ACC synthase and ACC oxidase. The increase in the rate of ethylene production and ACC synthase activity were suppressed by CO2 treatment and was dependent on CO2 concentration. CO2 treatment also suppressed wound-induced increase in ACC level irrespective of the CO2 concentration. The presence of 10% CO2 had no effect on ACC conjugation to MACC induced by wounding while 60% CO2 suppressed almost completely ACC conjugation. CO2 promoted and inhibited induction of ACC oxidase activity in excised tomato and winter squash tissues respectively. These results suggest that CO2 inhibits wound-induced ethylene biosynthesis by reducing the availability of ACC through repression of ACC synthase synthesis and/or inhibition of its activity. Also endogenous ethylene produced in response to wounding may play a role in the regulation of wound-induced ethylene production. © 1993.

    DOI: 10.1016/0925-5214(93)90024-W

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  • CHANGES IN L-PHENYLALANINE AMMONIA-LYASE ACTIVITY AND ANTHOCYANIN SYNTHESIS DURING BERRY RIPENING OF 3 GRAPE CULTIVARS

    KATAOKA, I, Y KUBO, A SUGIURA, T TOMANA

    JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE   52 ( 3 )   273 - 279   1983年

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    記述言語:英語   出版者・発行元:JAPAN SOC HORTICULTURAL SCI  

    DOI: 10.2503/jjshs.52.273

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講演・口頭発表等

  • モモ遺伝資源および交雑集団におけるモモ黒斑病の罹病性調査

    浅野貴洋, 河井崇, 伊藤那月, 芦田祐里, 飯島彩加, 鵜木悠治郎, 高田大輔, 中野龍平, 福田文夫, 久保康隆, 赤木剛士, 山本幹博, 牛島幸一郎

    園芸学研究 別冊  2020年 

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    開催年月日: 2020年

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  • モモ黒斑病罹病性に関連する遺伝子座領域の推定

    浅野貴洋, 河井崇, 鵜木悠治郎, 日原誠介, 高田大輔, 白澤健太, 赤木剛士, 福田文夫, 中野龍平, 久保康隆, 山本幹博, 牛島幸一郎

    育種学研究  2020年 

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    開催年月日: 2020年

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  • Screening of ripening related transcription factors using tomato DNA macroarray, 1-MCP and ripening impaired mutants

    日本ナス科・ウリ科ゲノム合同国際シンポジウム  2011年 

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  • Transcriptome profiling of ripening associated genes using tomato cDNA macroarray in ethylene and 1-MCP treated fruit

    8th Solanaceae and 2nd Cucurbitaceae Genome Joint Conference  2011年 

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  • 異形花型自家不和合性植物’ベニバナアマ’における短花柱花特異的発現遺伝子の単離

    園芸学会平成22年度春季大会  2010年 

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  • トマトDNAマクロアレイによるエチレン制御下にある果実成熟関連因子のスクリーニング

    園芸学会平成22年度秋季大会  2010年 

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  • SARK-IPTを導入したペチュニアおよびキク遺伝子組換え体における老化とストレス耐性に関する研究

    園芸学会平成22年度秋季大会  2010年 

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  • 貯蔵温度が’レインボーレッド’キウイの果実成熟に及ぼす影響

    園芸学会平成22年度秋季大会  2010年 

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  • Suppression of fruit softening by MA storage in 'Sanuki Gold' kiwifruit

    園芸学会平成22年度秋季大会  2010年 

     詳細を見る

  • Screening of ripening related genes using tomato DNA macroarray, 1-MCP and ripening impaired mutants

    Gordon Conference for Postharvest Physiology and Technology  2010年 

     詳細を見る

  • 低温によって誘導される‘さぬきゴールド’キウイ果実の成熟はエチレンから独立している

    園芸学会平成22年度秋季大会  2010年 

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  • トマトマイクロアレイを用いた果実成熟に関連する転写因子の探索

    園芸学会秋季大会  2008年 

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  • 一過的RNAサイレンシングを用いた果実および花における遺伝子機能の解析

    園芸学会秋季大会  2008年 

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  • Virus Induced Gene Sciencing法による成熟関連MADS-Box転写因子の探索

    園芸学会秋季大会  2008年 

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  • Coupling functional screen and macroarray for a comprehensive analysis of the secretomes associated with ripening-related changes in pear fruits

    園芸学会春季大会  2008年 

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  • ベニバナアマにおける異形花型自家不和合性関連遺伝子の探索

    園芸学会  2008年 

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  • EIN3/EIL抑制形質転換トマトの表現型解析

    園芸学会平成19年度春期大会  2007年 

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  • Characterization of ethylene biosynthesis and its regulation during fruit ripening in Actinidia chinensis var. Sanuki Gold.

    園芸学会平成19年度秋季大会  2007年 

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  • EIN3/EIL抑制形質転換トマトの表現型解析

    園芸学会平成19年度秋季大会  2007年 

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  • トマトマクロアレイを用いた成熟関連遺伝子の発現解析

    園芸学会平成19年度秋季大会  2007年 

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  • キウイフルーツ‘さぬきゴールド’の長期貯蔵の試み

    園芸学会平成19年度秋季大会  2007年 

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  • Honey Dew’果実におけるエチレン応答遺伝子の発現パターン

    園芸学会平成19年度秋季大会  2007年 

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  • 'Honey Dew'果実における成熟関連形質のエチレン応答性

    園芸学会平成18年度秋季大会  2006年 

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  • メロン品種における果実成熟特性とエチレン関連遺伝子の発現

    園芸学会平成18年度春季大会  2006年 

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  • セイヨウナシ果実におけるYSSTを用いた細胞外タンパク質をコードするcDNAのクローニング」

    園芸学会平成18年度春季大会  2006年 

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  • ‘中谷早生’‘刀根早生’、‘平核無’カキ果実の発育特性と収穫後の軟化防止

    園芸学会平成18年度秋季大会  2006年 

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  • 夏期の高温時における水ストレス緩和および1-メチルシクロプロペン処理によるカキ‘中谷早生’果実の軟化抑制

    園芸学会平成18年度春季大会  2006年 

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  • メロン果実の成熟に伴う軟化関連酵素遺伝子の発現解析

    園芸学会春季大会  2005年 

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  • Diversity of ethylene bisynthesis related to fruit ripening

    Gordon Conference (Postharvest Phsysiology and Technology)  2005年 

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  • ブドウ果実の成熟関連MADS遺伝子のクローニングとその発現および機能解析

    園芸学会春季大会  2005年 

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  • 環状はく皮処理および反射マルチの敷設がカキ‘刀根早生’果実の収穫後の軟化発生に及ぼす影響

    園芸学会春季大会  2005年 

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  • DNAマクロアレイを用いたトマト果実における成熟関連遺伝子の網羅的発現解析

    園芸学会秋季大会  2005年 

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  • メロン果実の軟化特性とPolygalacturonase遺伝子発現の系統間差異

    園芸学会秋季大会  2005年 

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  • 異なる方法で樹上脱渋した加温促成栽培カキ‘刀根早生’果実における収穫後の軟化発生と水ストレス緩和および1-MCP処理によるその抑制

    園芸学会秋季大会  2005年 

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  • DNAマクロアレイを用いたナス科野菜における連続光応答関連遺伝子の探索

    園芸学会秋季大会  2005年 

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  • 3種のナシ果実におけるb-galactosidaseとarabinofuranosidaseの酵素活性および遺伝子発現の解析

    園芸学会平成16年度秋季大会  2004年 

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  • セイヨウナシ成熟果の細胞壁多糖類の可溶化と低分子化におよぼす1-MCPの影響

    園芸学会平成16年度秋季大会  2004年 

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  • EIN3/EIL遺伝子抑制形質転換トマト果実におけるエチレン生合成の特性

    園芸学会平成16年度春季大会  2004年 

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  • カキのエチレン受容体遺伝子について

    園芸学会平成16年度春季大会  2004年 

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  • バナナ果実のMADS遺伝子のクローニングと成熟に伴う果皮および果肉間での発現比較

    園芸学会平成16年度春季大会  2004年 

     詳細を見る

  • セイヨウナシ、チュウゴクナシおよびニホンナシ果実成熟中のpolygalacturonase活性の変化

    園芸学会  2003年 

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  • イチジク果実の成熟中における細胞壁多糖類の部位別変化

    園芸学会  2003年 

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  • 油、オーキシン、傷害および成熟によって誘導されるイチジク果実のエチレン生成の調節機構

    園芸学会  2003年 

     詳細を見る

  • バナナの成熟関連遺伝子発現における果肉と果皮での1-MCP処理に対する反応の比較

    園芸学会  2003年 

     詳細を見る

  • カキ西条果実のCO2誘導エチレン生成

    園芸学会  2003年 

     詳細を見る

  • セイヨウナシ、チュウゴクナシ及びニホンナシ果実成熟中の細胞壁多糖類の低分子化および可溶化

    園芸学会  2003年 

     詳細を見る

  • RNA-interference法によって作出したEIN3/EIL遺伝子抑制形質転換トマトの解析

    園芸学会  2003年 

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  • セイヨウナシ果実の追熟に伴うβ-galactosidaseとarabinofuranosidase遺伝子のクローニングと発現解析

    園芸学会  2003年 

     詳細を見る

  • イチジク果実のエチレン生合成に及ぼす油、オーキシンおよびエスレル処理の影響

    園芸学会平成14年度春季大会  2002年 

     詳細を見る

  • MCP処理によるセイヨウナシ'ラ・フランス'と渋ガキ' 西条'果実の棚持ち期間の延長

    園芸学会平成14年度春季大会  2002年 

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  • EIN3ホモローグを抑制した形質転換トマトの作出

    園芸学会平成14年度秋期大会  2002年 

     詳細を見る

  • Expression analysis of gene encoding cell wall modifying enzymes during ripening in pear fruit

    XXXIth International Horicultural Congress  2002年 

     詳細を見る

  • Expression analysis of gene encoding cell wall modifying enzymes during postharvest period in fig fruit (Ficus carica cv. Masui Dauphine)

    XXXIth International Horicultural Congress  2002年 

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  • ABA処理したブドウ果実のアントシアニン蓄積とPAL遺伝子発現

    園芸学会平成14年度春季大会  2002年 

     詳細を見る

  • Differential regulation of ripening ethylene biosynthesis in several climacteric fruits

    International Ethylene Synposium III  2002年 

     詳細を見る

  • 成熟不全変異系統トマトにおけるエチレン生合成及び成熟関連遺伝子の発現解析.

    平成13年度園芸学会秋季大会  2001年 

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  • Tsukuba, September 28- October 1, 2001 Extended edible life of Charentais melon fruit by an inhibitor of ethylene action, 1-MCP.

    2nd International Symposium on Cucurbits  2001年 

     詳細を見る

  • トマトのエチレン情報伝達系遺伝子EIN3ホモローグのクローニングと解析.

    平成13年度園芸学会秋季大会  2001年 

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  • セイヨウナシ、チュウゴクナシ及びニホンナシにおけるPolygalacturonase遺伝子の発現比較.

    平成13年度園芸学会秋季大会  2001年 

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  • 1-メチルシクロプロパンによるカキ‘刀根早生’ハウス栽培果実の早期軟化抑制.

    平成13年度園芸学会秋季大会  2001年 

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  • 有孔ポリ袋包装と出荷容器の改善によるカキ‘刀根早生’促成栽培果実の軟化抑制.

    平成13年度園芸学会春季大会  2001年 

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  • セイヨウナシ果実のエクスパンシン遺伝子のクローニングと発現解析(第2報)(ポスター)

    平成13年度園芸学会秋季大会  2001年 

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  • セイヨウナシ果実の追熟に伴うPGおよびEGase遺伝子の全鎖長cDNAの単離とその発現解析

    園芸学会平成13年度春季大会  2001年 

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  • カキ果実における水ストレス誘導エチレン生合成の組織特異性.

    平成12年度園芸学会秋期大会  2000年 

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  • 有孔ポリ包装によるカキ‘刀根早生’促成栽培果実の流通中の軟化抑制技術.

    平成12年度園芸学会秋期大会  2000年 

     詳細を見る

  • Involvement of stress-induced ethylene biosynthesis in fruit softening of 'Saijo' persimmon fruit.

    2nd International Symposium on Persimmon  2000年 

     詳細を見る

  • アボカド果実のエチレン生合成関連遺伝子のクローニングとその発現.

    平成12年度園芸学会秋期大会  2000年 

     詳細を見る

  • Differential expression of genes involved in the biosynthesis in several climacteric fruits.

    Ethylene in Postharvest Physiology and Psthology  2000年 

     詳細を見る

  • バナナ果実のACC合成酵素活性測定法のPEG-アセトン洗浄抽出による確立.

    平成12年度園芸学会春季大会  2000年 

     詳細を見る

  • Cloning of genes encoding cell wall modifying enzymes and their expression in persimmon fruit.

    2nd International Symposium on Persimmon  2000年 

     詳細を見る

  • カキ果実における水ストレスによって誘導されるエチレン 生合成と軟化.

    日本植物生理学会2000年度年会  2000年 

     詳細を見る

  • カキ果実における水ストレス誘導エチレン生合成関連遺伝子の全鎖長cDNAの単離とその発現特性.

    平成12年度園芸学会春季大会  2000年 

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  • バナナのエチレン情報伝達系遺伝子ETRとEIN3ホモローグのクローニングと発現解析.

    平成12年度園芸学会春季大会  2000年 

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  • セイヨウナシ果実におけるエチレン生合成関連遺伝子の全鎖長cDNAの単離とその発現解析.

    平成12年度園芸学会春季大会  2000年 

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  • ポリエチレン包装によるハウス栽培カキ‘刀根早生’果実の収穫後の軟化防止の実用化.

    平成12年度園芸学会春季大会  2000年 

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受賞

  • 園芸学会賞論文賞

    2002年  

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    受賞国:日本国

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  • 園芸学会賞奨励賞

    1996年  

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    受賞国:日本国

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担当授業科目

  • Fruit Science (2021年度) 第3学期  - 金5,金6

  • Introduction to Applied Plant Science I (2021年度) 第1学期  - 火5,火6

  • コース演習3 (2021年度) 1・2学期  - その他

  • コース演習4 (2021年度) 3・4学期  - その他

  • 卒業論文 (2021年度) 1~4学期  - その他

  • 日本農業論1 (2021年度) 第1学期  - 水5~6

  • 日本農業論2 (2021年度) 第2学期  - 水5,水6

  • 日本農業論2 (2021年度) 第2学期  - 水5~6

  • 生物生産科学特別研究 (2021年度) 通年  - その他

  • 生物生産科学特別研究 (2021年度) 通年  - その他

  • 研究科目演習1 (2021年度) 1・2学期  - その他

  • 研究科目演習2 (2021年度) 3・4学期  - その他

  • 農産物代謝機構学 (2021年度) 前期  - その他

  • 農産物生理学演習 (2021年度) 前期  - その他

  • 農産物生理学演習 (2021年度) 後期  - その他

  • 農産物生理学演習 (2021年度) 後期  - その他

  • 農産物生理学演習 (2021年度) 後期  - その他

  • 農産物生理学演習 (2021年度) 前期  - その他

  • 農産物生理学演習 (2021年度) 前期  - その他

  • 農産物生理学特論 (2021年度) 後期  - 金3,金4

  • 農産物生理学1 (2021年度) 第1学期  - 木1,木2

  • 農産物生理学2 (2021年度) 第2学期  - 木1,木2

  • Advances in Plant Science (2021年度) 前期  - 火5,火6,金5,金6

  • Fruit Science(果実の科学) (2021年度) 第3学期  - 金5,金6

  • Fruit Science (2020年度) 第3学期  - 金5,金6

  • Introduction to Applied Plant Science I (2020年度) 第1学期  - 火5,火6

  • コース演習3 (2020年度) 1・2学期  - その他

  • コース演習4 (2020年度) 3・4学期  - その他

  • 卒業論文 (2020年度) 1~4学期  - その他

  • 日本農業論1 (2020年度) 第1学期  - 水5,水6

  • 日本農業論2 (2020年度) 第2学期  - 水5,水6

  • 日本農業論2 (2020年度) 第2学期  - 水5,水6

  • 生物生産科学特別研究 (2020年度) 通年  - その他

  • 研究科目演習1 (2020年度) 1・2学期  - その他

  • 研究科目演習2 (2020年度) 3・4学期  - その他

  • 農産物代謝機構学 (2020年度) 前期  - その他

  • 農産物生理学演習 (2020年度) 前期  - その他

  • 農産物生理学演習 (2020年度) 後期  - その他

  • 農産物生理学演習 (2020年度) 後期  - その他

  • 農産物生理学演習 (2020年度) 前期  - その他

  • 農産物生理学特論 (2020年度) 後期  - 金3,金4

  • 農産物生理学1 (2020年度) 第1学期  - 木1,木2

  • 農産物生理学2 (2020年度) 第2学期  - 木1,木2

  • 農産食品工学 (2020年度) 夏季集中  - その他

  • Advances in Plant Science (2020年度) 前期  - 火5,火6,金5,金6

  • Fruit Science(果実の科学) (2020年度) 第3学期  - 金5,金6

▼全件表示