記述言語：英語   掲載種別：研究論文（学術雑誌）  

A T217M heterozygous mutation in the SLC22A12 gene caused renal hypouricemia; this patient with IgA nephropathy had no findings other than IgA nephropathy on renal biopsy. Hypouricemia was susceptible to oxidative stress, but IgA nephropathy in the patient with hypouricemia could be treated with steroid pulse therapy without adverse events.

DOI： 10.1002/ccr3.9368

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

Cronkhite-Canada syndrome (CCS) is a non-hereditary disorder characterized by non-neoplastic hamartomatous gastrointestinal polyposis, hair loss, nail atrophy, hyperpigmentation, and diarrhea. While the relationship between CCS and nephritis remains unclear, seven cases of nephritis complicated by CCS have been reported to date, all of which were membranous nephropathy (MN). A 57-year-old man presented with taste disturbance, hair loss, nail plate atrophy, skin pigmentation, and frequent diarrhea. Endoscopic findings showed multiple polyposis of the stomach and large intestine. Given the above, he was diagnosed with CCS. The symptoms gradually improved with prednisolone treatment, although urinary protein and hypoproteinemia appeared during the tapering of prednisolone. He was diagnosed with MN using a renal biopsy, and immunofluorescence microscopy with IgG subclass staining showed predominantly diffuse granular capillary wall staining of IgG4. The cause of secondary MN was not found, including malignant tumors. Nephrotic-range proteinuria persisted despite treatment with prednisolone and cyclosporine. Additional treatment with mizoribine resulted in incomplete remission type 1 of nephrotic syndrome, suggesting that mizoribine may be a treatment option for patients with CCS with steroid-resistant MN. Considering a high prevalence of hypoproteinemia due to chronic diarrhea and protein-losing enteropathy in patients with CCS, proteinuria might be overlooked; thus, follow-up urinalysis would be recommended in patients with CCS.

DOI： 10.1007/s13730-024-00908-9

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

A 68-year-old woman was admitted to our hospital because of a rapid progression of renal dysfunction with positive myeloperoxidase antineutrophil cytoplasmic antibody and was diagnosed with rapidly progressive glomerulonephritis associated with microscopic polyangiitis (MPA). Severe right rectus sheath hematoma (RSH) bleeding from the inferior epigastric artery developed after starting hemodialysis, which required 4 transarterial embolizations due to recurrent bleeding. After additional treatment with methylprednisolone pulse therapy and rituximab, no rebleeding occurred. Although the giant hematoma reached the pelvis, it shrank spontaneously without any intervention. Nontraumatic RSH should therefore be considered when treating patients with multiple risk factors.

DOI： 10.2169/internalmedicine.3239-23

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1093/ndt/gfae078

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J-GLOBAL

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J-GLOBAL

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J-GLOBAL

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

RATIONALE & OBJECTIVE: Assessment of kidney biopsies provides crucial information for diagnosis and disease activity, as well as prognostic value. Kidney-biopsy specimens occasionally contain veno-muscular complex (VMC), which consists of muscle tissues around the kidney venous system in the corticomedullary region. However, the role of VMC and the clinical significance of VMC variants are poorly understood. In the present study, we investigated kidney prognostic values of VMC variants. STUDY DESIGN: Retrospective cohort study. SETTING & PARTICIPANTS: Among 808 patients who underwent a kidney biopsy from 2011 to 2019, 246 patients whose kidney biopsy specimens contained VMC were enrolled. PREDICTORS: VMC variants; inflammatory-VMC (an infiltration of ≥80 inflammatory cells/mm2-VMC area) and VMC hypertrophy (hyper-VMC, a VMC average width ≥850 μm), and the interstitial fibrosis/tubular atrophy (IFTA) score. OUTCOMES: A decline in estimated glomerular filtration rate (eGFR) ≥40% from the baseline or commencement of kidney replacement therapy. ANALYTICAL APPROACH: Cox proportional hazards model. RESULTS: Among 246 patients with data on VMC, mean baseline eGFR was 56.0±25.6 ml/min per 1.73 m2; 80 had high inflammatory-VMC, and 62 had VMC hypertrophy. There were 51 kidney events over median follow-up of 2.5 years. We analyzed 2 VMC variants. Multivariable logistic regression analysis revealed that eGFR negatively correlated with the presence of both inflammatory-VMC and hyper-VMC. A Cox proportional hazards analysis revealed that inflammatory-VMC (but not hyper-VMC) was independently associated with the primary outcome after adjustments for known risk factors of progression, including proteinuria, eGFR, and the interstitial fibrosis/tubular atrophy (IFTA) score (hazard ratio, 1.97; 95% confidence interval, 1.00-3.91). LIMITATIONS: Single-center study and small sample size. CONCLUSIONS: Assessment of inflammatory-VMC provides additional kidney prognostic information to known indicators of kidney disease progression in patients who undergo kidney biopsy. PLAIN-LANGUAGE SUMMARY: Assessment of kidney biopsies provides crucial information for diagnosis, disease activity, and prognostic value. Kidney-biopsy specimens occasionally contain veno-muscular complex (VMC), which consists of muscle tissues around the kidney venous system. Currently, the role of VMC in kidney health and diseases and the clinical significance of VMC variants are poorly understood. In the present study, we have shown that an infiltration of ≥80 inflammatory cells/mm2-VMC area (inflammatory-VMC) is independently associated with kidney disease progression after adjustments for known risk factors of progression. Therefore, assessment of inflammatory-VMC provides additional kidney prognostic information to known indicators of kidney disease progression in patients who undergo kidney biopsy.

DOI： 10.1016/j.xkme.2023.100733

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

Peritoneal fibrosis (PF) is a primary reason for discontinuing peritoneal dialysis, which involves characteristic changes of peritoneal mesothelial cells (PMCs). We previously reported preventive effects of implanting human epithelial-like PMCs (P-Epi) for mouse PF caused by mechanical peritoneum scrapings. In the present study, we analysed the preventive effects of culture supernatant of P-Epi in PF. Concentrated culture supernatant of P-Epi or human fibroblast-like PMCs (P-Fibro) or vehicles was injected into nude mice that had undergone mechanical scraping of the parietal and visceral peritoneum, and thickness and amount of adhesions were analysed. Although increased peritoneal adhesions and peritoneum thickening were observed in the vehicle-injected positive control group compared to the sham operation group, fewer number of adhesions and less thickness were observed in the mice treated with culture supernatant of P-Epi, but not P-Fibro, compared to the vehicle-injected positive controls. Immunofluorescent analysis revealed that the expression of extracellular matrix, type I collagen and fibronectin, was lower in the mice treated with culture supernatant of P-Epi than in the vehicle-injected positive controls. In addition, exosomes from P-Epi significantly reduced transforming growth factor-β (TGF-β)-induced expressions of type I collagen and fibronectin in 3T3 fibroblast cells. Collectively, culture supernatant of P-Epi has preventive effects on PF, thus cell therapy is not necessarily required. Further exploration of substances secreted by P-Epi and their protective mechanisms could lead to the development of therapeutic strategies to limit PF.

DOI： 10.1177/08968608231213577

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

Kidney diseases are worldwide public health problems affecting millions of people. However, there are still limited therapeutic options against kidney diseases. Semaphorin 3A (SEMA3A) is a secreted and membrane-associated protein, which regulates diverse functions, including immune regulation, cell survival, migration and angiogenesis, thus involving in the several pathogeneses of diseases, including eyes and neurons, as well as kidneys. SEMA3A is expressed in podocytes and tubular cells in the normal adult kidney, and recent evidence has revealed that excess SEMA3A expression and the subsequent signaling pathway aggravate kidney injury in a variety of kidney diseases, including nephrotic syndrome, diabetic nephropathy, acute kidney injury, and chronic kidney disease. In addition, several reports have demonstrated that the inhibition of SEMA3A ameliorated kidney injury via a reduction in cell apoptosis, fibrosis and inflammation; thus, SEMA3A may be a potential therapeutic target for kidney diseases. In this review article, we summarized the current knowledge regarding the role of SEMA3A in kidney pathophysiology and their potential use in kidney diseases.

DOI： 10.3390/diagnostics13193038

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

Acute kidney injury (AKI) is a clinical syndrome where a rapid decrease in kidney function and/or urine output is observed, which may result in the imbalance of water, electrolytes and acid base. It is associated with poor prognosis and prolonged hospitalization. Therefore, an early diagnosis and treatment to avoid the severe AKI stage are important. While several biomarkers, such as urinary L-FABP and NGAL, can be clinically useful, there is still no gold standard for the early detection of AKI and there are limited therapeutic options against AKI. miRNAs are non-coding and single-stranded RNAs that silence their target genes in the post-transcriptional process and are involved in a wide range of biological processes. Recent accumulated evidence has revealed that miRNAs may be potential biomarkers and therapeutic targets for AKI. In this review article, we summarize the current knowledge about miRNAs as promising biomarkers and potential therapeutic targets for AKI, as well as the challenges in their clinical use.

DOI： 10.3390/diagnostics13182893

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掲載種別：研究論文（学術雑誌）   出版者・発行元：American College of Physicians  

DOI： 10.7326/aimcc.2022.1003

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

INTRODUCTION: While chronic kidney disease (CKD) is one of the most important contributors to mortality from non-communicable diseases, the number of nephrologists is limited worldwide. Medical cooperation is a system of cooperation between primary care physicians and nephrological institutions, consisting of nephrologists and multidisciplinary care teams. Although it has been reported that multidisciplinary care teams contribute to the prevention of worsening renal functions and cardiovascular events, there are few studies on the effect of a medical cooperation system. METHODS: We aimed to evaluate the effect of medical cooperation on all-cause mortality and renal prognosis in patients with CKD. One hundred and sixty-eight patients who visited the one hundred and sixty-three clinics and seven general hospitals of Okayama city were recruited between December 2009 and September 2016, and one hundred twenty-three patients were classified into a medical cooperation group. The outcome was defined as the incidence of all-cause mortality, or renal composite outcome (end-stage renal disease or 50% eGFR decline). We evaluated the effects on renal composite outcome and pre-ESRD mortality while incorporating the competing risk for the alternate outcome into a Fine-Gray subdistribution hazard model. RESULTS: The medical cooperation group had more patients with glomerulonephritis (35.0% vs. 2.2%) and less nephrosclerosis (35.0% vs. 64.5%) than the primary care group. Throughout the follow-up period of 5.59 ± 2.78 years, 23 participants (13.7%) died, 41 participants (24.4%) reached 50% decline in eGFR, and 37 participants (22.0%) developed end-stage renal disease (ESRD). All-cause mortality was significantly reduced by medical cooperation (sHR 0.297, 95% CI 0.105-0.835, p = 0.021). However, there was a significant association between medical cooperation and CKD progression (sHR 3.069, 95% CI 1.225-7.687, p = 0.017). CONCLUSION: We evaluated mortality and ESRD using a CKD cohort with a long-term observation period and concluded that medical cooperation might be expected to influence the quality of medical care in the patients with CKD.

DOI： 10.3390/jpm13040582

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担当区分：筆頭著者,　責任著者   記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

超音波ガイド経皮的針腎生検は,種々の腎臓病の診断および活動性の評価のために必要な検査であるが,出血などの合併症のリスクが高い侵襲的な技術であり,実用的なシミュレーションモデルが望まれる。われわれは鶏卵を用いた簡易的腎生検シミュレーターを開発したが,耐久性が低く長期保存が困難であった。今回われわれは幅広く適用されるモデルを目指して,スチレン系軟質樹脂を用いた新たな腎生検シミュレーターを開発した。作製したモデルは,超音波下で腎臓の皮質・髄質に該当する部分のコントラストは実際の腎臓のものに類似しており,針穿刺の際にモデル内に針が侵入する様子が超音波下で観察可能であった。本モデルの検証のため,岡山大学病院の腎生検経験20例未満の研修生10名に腎生検模擬を実施し,腎生検経験20例以上の腎生検に熟達した腎臓内科医20名がその様子を観察あるいは模擬を実施した。また,腎生検模擬後に全参加者にアンケート調査を実施した。90%の研修生が,今回の腎生検模擬によって患者への腎生検に対する不安が軽減されると回答した。また,すべての参加者が,研修生が本腎生検模擬を行うことを推奨すると回答した。耐久性の評価では,約100回穿刺後の形態および超音波下の画像ともに継続使用可能と考えられた。以上から,開発したシミュレーターは腎生検の実用的なシミュレーションモデルの良い候補になり得る。(著者抄録)

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

While hypothyroidism increases serum creatinine (Cr) levels, it is uncertain whether the elevation is mediated via a decline in the glomerular filtration rate (GFR) or the reflection of enhanced Cr production from the muscles or both. In the present study, we explored an association between urinary Cr excretion rate (CER) and hypothyroidism. A total of 553 patients with chronic kidney disease were enrolled in a cross-sectional study. Multiple linear regression analysis was performed to explore the association between hypothyroidism and urinary CER. The mean urinary CER was 1.01 ± 0.38 g/day and 121 patients (22%) had hypothyroidism. The multiple linear regression analysis revealed explanatory variables with urinary CER, including age, sex, body mass index, 24 h Cr clearance (24hrCcr), and albumin while hypothyroidism was not considered an independent explanatory variable. In addition, scatter plot analysis with regression fit line representing the association between estimated GFR calculated using s-Cr (eGFRcre) and 24hrCcr revealed that eGFRcre and 24hrCcr had strong correlations with each other in hypothyroid patients as well as euthyroid patients. Collectively, hypothyroidism was not considered an independent explanatory variable for urinary CER in the present study and eGFRcre is a useful marker to evaluate kidney function regardless of the presence of hypothyroidism.

DOI： 10.3390/diagnostics13040669

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担当区分：筆頭著者,　責任著者   記述言語：日本語   出版者・発行元：(公社)日本透析医会  

腎臓線維化は慢性腎臓病進展における共通経路として知られるが,治療法は限られており,新規機序の薬物創出が求められている.Semaphorin3A(SEMA3A)は細胞間の情報伝達物質として機能する分泌型蛋白であり,細胞増殖,血管新生,免疫細胞の制御など多彩な生理活性作用を持つ.SEMA3Aは腎臓では糸球体上皮細胞および尿細管上皮細胞に発現し,腎臓病進展との関連性が報告されるがその機序は不詳である.本研究では一側尿管結紮(unilateral ureteral obstruction;UUO)マウスモデルを用いて,SEMA3Aと腎臓線維化の関連およびSEMA3A阻害剤の効果を検討した.UUOマウスの腎臓にはSEMA3Aおよびその受容体のNeuropilin-1(NRP1)発現が亢進しており,SEMA3A阻害剤によりUUOによる腎臓線維化は有意に軽減した.TGF-β1により誘導される培養ヒト尿細管上皮細胞の上皮間葉転換(epithelial-mesenchymal transition;EMT)はSEMA3A阻害剤により抑制された.JNK:c-Jun N-terminal kinase阻害剤(SP600125)はSEMA3Aにより誘導されるEMTを抑制したことから,JNKシグナルがSEMA3A経路による腎臓線維化に関与すると考えられた.さらに培養線維芽細胞にSEMA3Aを添付すると線維化マーカーの発現亢進が認められた.以上より腎臓線維化に対するSEMA3Aを制御する新たな治療戦略となりうることが示された.(著者抄録)

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.2169/internalmedicine.0966-22

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記述言語：日本語   出版者・発行元：(一社)日本透析医学会  

医学論文数は2000年に入り急増し,増加に伴った情報氾濫が起きており,その適切な情報の取捨選択が重要である.今回われわれは医中誌Webに収蔵されている血液透析の原著論文および症例事例論文の要約をテキストマイニングし解析を行った.医学中央雑誌刊行会にて運営されている医中誌Webにて検索した,1979年以降に収載された「血液透析」の原著論文は26,408報であり,症例事例論文は8,936報であった.それらの原著論文の要旨・抄録を抽出した延べ単語総数は1,637,446語であり,症例事例論文は562,949語であった.原著論文解析では体外循環法や効率,生体適合性などの単語が頻出したのに対し,症例事例論文では実臨床の治療難渋例関連の単語の頻出が多く認められた.以上より医中誌Web収載の血液透析関連の原著論文および症例事例論文に対しテキストマイニングを用いて解析を行い,全論文から血液透析の実臨床の変遷を把握することが可能であった.(著者抄録)

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1038/s41598-022-19226-0

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/nep.14079

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記述言語：日本語   出版者・発行元：(株)東京医学社  

医学中央雑誌に収録された慢性腎臓病に関する症例・事例論文について、人工知能(AI)を用いてテキストマイニングと情報解析を行った。その結果、治療難渋例の報告が多いため透析に関する単語が多く、腎疾患名は糖尿病性腎症、慢性糸球体腎炎、腎硬化症が多かった。

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1097/MD.0000000000028872

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1155/2022/3157841

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.32604/biocell.2022.018594

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.3389/fendo.2022.1048863

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.3389/fcell.2022.892356

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

Renal fibrosis is the common pathological pathway in progressive renal diseases. In the study, we analyzed the roles of Semaphorin 3A (SEMA3A) on renal fibrosis and the effect of SEMA3A-inhibitor (SEMA3A-I) using unilateral ureteral obstruction (UUO) mouse model. The expression of SEMA3A in the proximal tubulus and neuropilin-1 (NRP1), a recepor of SEMA3A, in fibloblast and tubular cells were increased in the UUO kidneys. The increased expression of myofibroblast marker tenascin-C and fibronection as well as renal fibrosis were increased in UUO kidneys, all of which were ameliorated by SEMA3A-I. In addition, c-Jun N-terminal kinase (JNK) signaling pathway known as the target of SEMA3A signaling, was activated in proximal tubular cells and fibroblast cells after UUO surgery while SEMA3A-I significantly attenuated the activation. In vitro, treatments with SEMA3A as well as transforming growth factor-β1 (TGF-β1) in human proximal tubular cells lost epithelial cell characters while SEMA3A-I significantly ameliorated this transformation. JNK inhibitor, SP600125, partially reversed SEMA3A and TGF-β1-induced cell transformation, indicating that JNK signaling is involved in SEMA3A-induced renal fibrosis. In addition, the treatment with SEMA3A in fibroblast cells activated the expression of tenascin-C, collagen type I and fibronection, indicating that SEMA3A may accelerate renal fibrosis through the activation of fibroblast cells. The analysis of human data revealed the positive correlation between urinary SEMA3A and urinary N-acetyl-β-D-glucosaminidase, indicating the association between SEMA3A and tubular injury. In conclusion, SEMA3A signaling is involved in renal fibrosis through JNK signaling pathway and SEMA3A-I might be the therapeutic option for protecting from renal fibrosis.

DOI： 10.1152/ajprenal.00234.2021

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

医学の進歩は著しく,腎臓疾患の概念や診断・治療も大きく変化している。医学論文数は2000年に入り急増し,増加に伴った情報氾濫が起きており,その適切な情報の取捨選択は限られた時間の中では困難である。今回われわれは医学中央雑誌に収蔵されている腎臓領域の原著論文の要約を全てテキストマイニングし解析を行った。医学中央雑誌にて検索した1982年以降に収載された「慢性腎臓病」関連の論文数(症例報告・事例を除く)は12,801報であった。それらの原著論文の要約・アブストラクトから抽出した述べ単語総数は8,556,712語であった。「慢性腎臓病」で医学中央雑誌から論文を抽出しているにも関わらず,上位に挙がった単語には「透析」,「血液透析」などが「慢性腎臓病」,「慢性腎不全」よりも出現頻度は高かった。単語のネットワーク構造解析では,「透析」,「血圧」,「慢性腎臓病」,「透析条件」などのグループが認められ,トピックとして多くの論文があることが示唆された。単語頻度推移解析では年代ごとの出現単語頻度を解析したところ,「慢性腎臓病」や慢性腎臓病の機能を評価する「eGFR」は2005年以降に多く記載されており,「慢性腎不全」は1982年以降認められていたが,2002年以降には徐々にその割合は減少しており,慢性腎不全といった腎機能低下を示す包括的な単語使用から,慢性腎臓病の提唱により単語出現頻度が移行していることが示唆された。以上より「慢性腎臓病」関連論文では透析に関係する単語が多く抽出された。一方,各糸球体性疾患の病名は多岐にわたり,反対に末期腎不全の腎代替療法は血液透析や腹膜透析に集約されている可能性も示唆された。医学中央雑誌に収載されている慢性腎臓病関連の原著論文をテキストマイニングの手法を用いて解析を行い,慢性腎臓病関連の変動を把握することが可能であった。(著者抄録)

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担当区分：責任著者   記述言語：英語  

DOI： 10.1111/nep.13966

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掲載種別：研究論文（学術雑誌）  

Introduction: For human, the resolution of images is important for diagnosis. Many clinical applications of artificial intelligence have been studied, however there are few reports on the difference in diagnosis between humans and artificial intelligence on the point of the renal pathological image resolution. Objectives: We examined whether the resolution of renal pathological images affects diagnosis of artificial intelligence and human. Patients and Methods: From 885 renal biopsy patients, we collected renal IgA immunofluorescent pathological images that resolution is 4, 16, 32, 64, 128, 256 and 512 pixels for each patient, and divided into training data set and validation data set, and created optimum deep learning models for each resolution. To compare with artificial intelligence nephrologist also tried to diagnose by using the same validation data set images. Results: We inputted IgA immunofluorescent pathological images into each optimum model. Human could not identify specific staining site with four pixels images, however, each resolution optimum model showed high accuracy, average over 80%. The each accuarcy was observed higher depending on the resolution. The area under the curve (AUC) showed higher diagnosis ratio depending on the resolution, too. Nephrologist performed high diagnosis sensitivity depending on resolution images as same as artificial intelligence. However, nephrologists’ diagnosis observed large variations in specificity depending on resolution. These results suggested that the resolution might affect specificity for human not artificial intelligence Conclusion: The resolution of images might be important for not AI but human on the point of specificity.

DOI： 10.34172/jnp.2021.26

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Several large clinical trials have shown renal and cardioprotective effects of sodium-glucose cotransporter 2 (SGLT2) inhibitors in diabetes patients, and the protective mechanisms need to be elucidated. There have been accumulating studies which report that SGLT2 inhibitors ameliorate autophagy deficiency of multiple organs. In overnutrition diseases, SGLT2 inhibitors affect the autophagy via various signaling pathways, including mammalian target of rapamycin (mTOR), sirtuin 1 (SIRT1), and hypoxia-inducible factor (HIF) pathways. Recently, it turned out that not only stagnation but also overactivation of autophagy causes cellular damages, indicating that therapeutic interventions which simply enhance or stagnate autophagy activity might be a "double-edged sword" in some situations. A small number of studies suggest that SGLT2 inhibitors not only activate but also suppress the autophagy flux depending on the situation, indicating that SGLT2 inhibitors can "regulate" autophagic activity and help achieve the appropriate autophagy flux in each organ. Considering the complicated control and bilateral characteristics of autophagy, the potential of SGLT2 inhibitors as the regulator of autophagic activity would be beneficial in the treatment of autophagy deficiency.

DOI： 10.3389/fphar.2021.761842

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

Nephrotic syndrome represents the clinical situation characterized by presence of massive proteinuria and low serum protein caused by a variety of diseases, including minimal change nephrotic syndrome (MCNS), focal segmental glomerulosclerosis (FSGS) and membranous glomerulonephropathy. Differentiating between diagnoses requires invasive renal biopsies in general. Even with the biopsy, we encounter difficulties to differentiate MCNS and FSGS in some cases. There is no other better option currently available for the diagnosis other than renal biopsy. MicroRNAs (miRNAs) are no-coding RNAs of approximately 20 nucleotides in length, which regulate target genes in the post-transcriptional processes and have essential roles in many diseases. MiRNAs in serum and urine have been shown as non-invasive biomarkers in multiple diseases, including renal diseases. In this article, we summarize the current knowledge of miRNAs as the promising biomarkers for nephrotic syndrome.

DOI： 10.3390/ijms22010088

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担当区分：責任著者   記述言語：英語  

DOI： 10.1016/j.ekir.2020.06.010

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担当区分：筆頭著者,　責任著者   記述言語：英語  

DOI： 10.1177/0961203320935988

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1097/RHU.0000000000001505

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Artificial Intelligence (AI) imaging diagnosis is developing, making enormous steps forward in medical fields. Regarding diabetic nephropathy (DN), medical doctors diagnose them with clinical course, clinical laboratory data and renal pathology, mainly evaluate with light microscopy images rather than immunofluorescent images because there are no characteristic findings in immunofluorescent images for DN diagnosis. Here, we examined the possibility of whether AI could diagnose DN from immunofluorescent images. We collected renal immunofluorescent images from 885 renal biopsy patients in our hospital, and we created a dataset that contains six types of immunofluorescent images of IgG, IgA, IgM, C3, C1q and Fibrinogen for each patient. Using the dataset, 39 programs worked without errors (Area under the curve (AUC): 0.93). Five programs diagnosed DN completely with immunofluorescent images (AUC: 1.00). By analyzing with Local interpretable model-agnostic explanations (Lime), the AI focused on the peripheral lesion of DN glomeruli. On the other hand, the nephrologist diagnostic ratio (AUC: 0.75833) was slightly inferior to AI diagnosis. These findings suggest that DN could be diagnosed only by immunofluorescent images by deep learning. AI could diagnose DN and identify classified unknown parts with the immunofluorescent images that nephrologists usually do not use for DN diagnosis.

DOI： 10.3390/diagnostics10070466

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

Adult kidney stem cells are known to have important roles in renal regeneration after acute kidney injury. Although trophic factors from tissue stem cells have been reported to promote the regeneration of other organs, there is limited number of evidence of this phenomenon in the kidneys. Here, we explored the effects of secreted factors from kidney stem cells. We intraperitoneally administered culture supernatant obtained from adult rat kidney stem/progenitor cells into rat kidney ischemia/reperfusion injury models, and the treatment significantly ameliorated renal tubulointerstitial injury, suppressed tubular cell apoptosis, diminished inflammation and promoted the proliferation of both residual renal cells and immature cells. In vitro, treatment with culture supernatant from kidney stem cells significantly promoted cell proliferation and suppressed cisplatin-induced cell apoptosis in both normal rat kidney cells and kidney stem cells. In addition, treatment with culture supernatant increased the expression of nestin in normal rat kidney cells, suggesting the dedifferentiation of tubular cells into stem-like cells. Analysis of the culture supernatant revealed that it contained a variety of growth factors. Taken together, the results suggest that these factors together lead to renal regeneration. In conclusion, adult kidney stem cells contribute to renal regeneration indirectly through the secretion of regenerative factors.

DOI： 10.1016/j.scr.2020.101865

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Podocyte injury is an independent risk factor for the progression of renal diseases. Semaphorin3A (SEMA3A), expressed in podocytes and tubular cells in the mammalian adult kidneys, has been reported to regulate diverse biological functions and be associated with renal diseases. Here, we investigated pathological roles of SEMA3A signaling on podocyte injury using a doxorubicin (Dox)-induced mouse model and examined the therapeutic effect of SEMA3A-inhibitor (SEMA3A-I). We demonstrated that Dox caused massive albuminuria and podocyte apoptosis as well as an increase of SEMA3A expression in podocytes, all of which were ameliorated with SEMA3A-I treatment. In addition, c-Jun N-terminal kinase (JNK), known as a downstream of SEMA3A signaling, was activated in Dox-injected mouse podocytes while SEMA3A-I treatment partially blocked the activation. In vitro, SEMA3A-I protected against Dox-induced podocyte apoptosis and recombinant SEMA3A caused podocyte apoptosis with activation of JNK signaling. JNK inhibitor, SP600125, attenuated SEMA3A-induced podocyte apoptosis, indicating that the JNK pathway would be involved in SEMA3A-induced podocyte apoptosis. Furthermore, the analysis of human data revealed a positive correlation between levels of urinary SEMA3A and protein, suggesting that SEMA3A is associated with podocyte injury. In conclusion, SEMA3A has essential roles in podocyte injury and it would be the therapeutic target for protecting from podocyte injury.

DOI： 10.3390/ijms21114099

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Obesity is supposed to cause renal injury via autophagy deficiency. Recently, sodium glucose co-transporter 2 inhibitors (SGLT2i) were reported to protect renal injury. However, the mechanisms of SGLT2i for renal protection are unclear. Here, we investigated the effect of SGLT2i for autophagy in renal proximal tubular cells (PTCs) on obesity mice. We fed C57BL/6J mice with a normal diet (ND) or high-fat and -sugar diet (HFSD) for nine weeks, then administered SGLT2i, empagliflozin, or control compound for one week. Each group contained N = 5. The urinary N-acetyl-beta-d-glucosaminidase level in the HFSD group significantly increased compared to ND group. The tubular damage was suppressed in the SGLT2i-HFSD group. In electron microscopic analysis, multi lamellar bodies that increased in autophagy deficiency were increased in PTCs in the HFSD group but significantly suppressed in the SGLT2i group. The autophagosomes of damaged mitochondria in PTCs in the HFSD group frequently appeared in the SGLT2i group. p62 accumulations in PTCs were significantly increased in HFSD group but significantly suppressed by SGLT2i. In addition, the mammalian target of rapamycin was activated in the HFSD group but significantly suppressed in SGLT2i group. These data suggest that SGLT2i has renal protective effects against obesity via improving autophagy flux impairment in PTCs on a HFSD.

DOI： 10.3390/ijms21114054

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

BACKGROUND: The advent of immune checkpoint inhibitors (ICIs) has significantly improved the prognosis of patients with advanced malignancies. On the other hand, these drugs might cause immune-related adverse events (irAEs) including endocrinopathies and nephropathies. Thyroid dysfunction is one of the most common irAEs. For ICIs-induced nephropathies, most cases are due to tubulointerstitial nephritis, which might require steroid treatment. Here, we report a patient with non-small cell lung cancer treated with ICI who developed increased serum creatinine (s-Cr) levels due to ICIs-induced hypothyroidism. CASE PRESENTATION: A 57-year-old Asian man with refractory non-small cell lung cancer under ICIs therapy (pembrolizumab, an anti-programmed cell death-1 monoclonal antibody) developed increased s-Cr levels 5 months after the pembrolizumab initiation. His laboratory data, renal biopsy, and Gallium-67 scintigraphy findings denied pembrolizumab-induced tubulointerstitial nephritis. His renal function was correlated with thyroid function. Despite the increase of s-Cr levels, serum cystatin C levels were normal, which could be explained by the hypothyroidism. Levothyroxine treatment improved renal function as well as thyroid function. Then pembrolizumab was resumed, and both his thyroid and renal function remained normal level. Ultimately, we concluded that the increased s-Cr levels were caused by pembrolizumab-induced hypothyroidism. CONCLUSION: All clinicians involved in ICI treatment need to recognize the possible increase in s-Cr levels caused by ICIs-induced hypothyroidism, and we propose monitoring serum cystatin C levels to differentiate ICIs-induced hypothyroidism from tubulointerstitial nephritis before invasive renal biopsies or steroid treatment, which are recommended by the prescribing information for pembrolizumab, are performed.

DOI： 10.1186/s12882-020-01775-z

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

Mesenchymal stem cells (MSCs) have immunomodulatory and regenerative effects in many organs, including the kidney. Emerging evidence has shown that the trophic effects from MSCs are mainly mediated by the paracrine mechanism rather than the direct differentiation of MSCs into injured tissues. These secretomes from MSCs include cytokines, growth factors, chemokines and extracellular vesicles (EVs) containing microRNAs, mRNAs, and proteins. Many research studies have revealed that secretomes from MSCs have potential to ameliorate renal injury in renal disease models, including acute kidney injury and chronic kidney disease through a variety of mechanisms. These trophic mechanisms include immunomodulatory and regenerative effects. In addition, accumulating evidence has uncovered the specific factors and therapeutic mechanisms in MSC-derived EVs. In this article, we summarize the recent advances of immunomodulatory and regenerative effects of EVs from MSCs, especially focusing on the microRNAs.

DOI： 10.3390/ijms21030756

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

BACKGROUND: Necroptosis is a newly discovered cell death pathway that plays a critical role in AKI. The involvement of integrin-linked kinase (ILK) in necroptosis has not been studied. METHODS: We performed experiments in mice with an Ilk deletion in collecting duct (CD) principal cells (PCs), and cultured tubular epithelial cells treated with an ILK inhibitor or ILK siRNA knockdown. RESULTS: Ilk deletion in CD PCs resulted in acute tubular injury and early mortality in mice. Progressive interstitial fibrosis and inflammation associated with the activation of the canonical TGF-β signaling cascade were detected in the kidneys of the mice lacking ILK in the CD PCs. In contrast to the minimal apoptosis detected in the animals' injured CDs, widespread necroptosis was present in ILK-deficient PCs, characterized by cell swelling, deformed mitochondria, and rupture of plasma membrane. In addition, ILK deficiency resulted in increased expression and activation of necroptotic proteins MLKL and RIPK3, and membrane translocation of MLKL in CD PCs. ILK inhibition and siRNA knockdown reduced cell survival in cultured tubular cells, concomitant with increased membrane accumulation of MLKL and/or phospho-MLKL. Administration of a necroptosis inhibitor, necrostatin-1, blocked cell death in vitro and significantly attenuated inflammation, interstitial fibrosis, and renal failure in ILK-deficient mice. CONCLUSIONS: The study demonstrates the critical involvement of ILK in necroptosis through modulation of the RIPK3 and MLKL pathway and highlights the contribution of CD PC injury to the development of inflammation and interstitial fibrosis of the kidney.

DOI： 10.1681/ASN.2018111162

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Cardiovascular disease constitutes the leading cause of mortality in patients with chronic kidney disease (CKD) and end-stage renal disease. Despite increasing recognition of a close interplay between kidney dysfunction and cardiovascular disease, termed cardiorenal syndrome (CRS), the underlying mechanisms of CRS remain poorly understood. Here we report the development of pathological cardiac hypertrophy and fibrosis in early stage non-uremic CKD. Moderate kidney failure was induced three weeks after unilateral urinary obstruction (UUO) in mice. We observed pathological cardiac hypertrophy and increased fibrosis in UUO-induced CKD (UUO/CKD) animals. Further analysis indicated that this cardiac fibrosis was associated with increased expression of transforming growth factor β (TGF-β) along with significant upregulation of Smad 2/3 signaling in the heart. Moreover early treatment of UUO/CKD animals with an angiotensin-converting-enzyme inhibitor (ACE I), Enalapril, significantly attenuated cardiac fibrosis. Enalapril antagonized activation of the TGF-β signaling pathway in the UUO/CKD heart. In summary our study demonstrates the presence of pathological cardiac hypertrophy and fibrosis in mice early in UUO-induced CKD, in association with early activation of the TGF-β/Smad signaling pathway. We also demonstrate the beneficial effect of ACE I in alleviating this early fibrogenic process in the heart in UUO/CKD animals.

DOI： 10.1038/s41598-018-34216-x

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Aquaporin-2 (AQP2) is a water channel protein expressed in principal cells (PCs) of the kidney collecting ducts (CDs) and plays a critical role in mediating water reabsorption and urine concentration. AQP2 undergoes both regulated trafficking mediated by vasopressin (VP) and constitutive recycling, which is independent of VP. For both pathways, actin cytoskeletal dynamics is a key determinant of AQP2 trafficking. We report here that manganese chloride (MnCl2) is a novel and potent regulator of AQP2 trafficking in cultured cells and in the kidney. MnCl2 treatment promoted internalization and intracellular accumulation of AQP2. The effect of MnCl2 on the intracellular accumulation of AQP2 was associated with activation of RhoA and actin polymerization without modification of AQP2 phosphorylation. Although the level of total and phosphorylated AQP2 did not change, MnCl2 treatment impeded VP-induced phosphorylation of AQP2 at its serine-256, -264, and -269 residues and dephosphorylation at serine 261. In addition, MnCl2 significantly promoted F-actin polymerization along with downregulation of RhoA activity and prevented VP-induced membrane accumulation of AQP2. Finally, MnCl2 treatment in mice resulted in significant polyuria and reduced urinary concentration, likely due to intracellular relocation of AQP2 in the PCs of kidney CDs. More importantly, the reduced urinary concentration caused by MnCl2 treatment in animals was not corrected by VP. In summary, our study identified a novel effect of MnCl2 on AQP2 trafficking through modifying RhoA activity and actin polymerization and uncovered its potent impact on water diuresis in vivo.

DOI： 10.1152/ajprenal.00391.2017

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

A kidney has the ability to regenerate itself after a variety of renal injuries. Mesenchymal stem cells (MSCs) have been shown to ameliorate tissue damages during renal injuries and diseases. The regenerations induced by MSCs are primarily mediated by the paracrine release of soluble factors and extracellular vesicles, including exosomes and microvesicles. Extracellular vesicles contain proteins, microRNAs, and mRNAs that are transferred into recipient cells to induce several repair signaling pathways. Over the past few decades, many studies identified trophic factors from MSCs, which attenuate renal injury in a variety of animal acute kidney injury models, including renal ischemia-reperfusion injury and drug-induced renal injury, using microarray and proteomic analysis. Nevertheless, these studies have revealed the heterogeneity of trophic factors from MSCs that depend on the cell origins and different stimuli including hypoxia, inflammatory stimuli, and aging. In this review article, we summarize the secretomes and regenerative mechanisms induced by MSCs and highlight the possible heterogeneity of trophic factors from different types of MSC and different circumstances for renal regeneration.

DOI： 10.1155/2018/8693137

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1152/ajprenal.00038.2017

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：NATURE PUBLISHING GROUP  

The glomerulus exercises its filtration barrier function by establishing a complex filtration apparatus consisting of podocyte foot processes, glomerular basement membrane and endothelial cells. Disruption of any component of the glomerular filtration barrier leads to glomerular dysfunction, frequently manifested as proteinuria. Ultrastructural studies of the glomerulus by transmission electron microscopy (TEM) and conventional scanning electron microscopy (SEM) have been routinely used to identify and classify various glomerular diseases. Here we report the application of newly developed helium ion scanning microscopy (HIM) to examine the glomerulopathy in a Col4a3 mutant/Alport syndrome mouse model. Our study revealed unprecedented details of glomerular abnormalities in Col4a3 mutants including distorted podocyte cell bodies and disorganized primary processes. Strikingly, we observed abundant filamentous microprojections arising from podocyte cell bodies and processes, and presence of unique bridging processes that connect the primary processes and foot processes in Alport mice. Furthermore, we detected an altered glomerular endothelium with disrupted sub-endothelial integrity. More importantly, we were able to clearly visualize the complex, three-dimensional podocyte and endothelial interface by HIM. Our study demonstrates that HIM provides nanometer resolution to uncover and rediscover critical ultrastructural characteristics of the glomerulopathy in Col4a3 mutant mice.

DOI： 10.1038/s41598-017-12064-5

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：COMPANY OF BIOLOGISTS LTD  

The water channel aquaporin-2 (AQP2) is a major regulator of water homeostasis in response to vasopressin (VP). Dynamic trafficking of AQP2 relies on its close interaction with trafficking machinery proteins and the actin cytoskeleton. Here, we report the identification of ezrin, an actin-binding protein from the ezrin/radixin/moesin (ERM) family as an AQP2-interacting protein. Ezrin was first detected in a co-immunoprecipitation (co-IP) complex using an anti-AQP2 antibody in a proteomic analysis. Immunofluorescence staining revealed the co-expression of ezrin and AQP2 in collecting duct principal cells, and VP treatment caused redistribution of both proteins to the apical membrane. The ezrin-AQP2 interaction was confirmed by co-IP experiments with an anti-ezrin antibody, and by pulldown assays using purified full-length and FERM domain-containing recombinant ezrin. By using purified recombinant proteins, we showed that ezrin directly interacts with AQP2 C-terminus through its N-terminal FERM domain. Knocking down ezrin expression with shRNA resulted in increased membrane accumulation of AQP2 and reduced AQP2 endocytosis. Therefore, through direct interaction with AQP2, ezrin facilitates AQP2 endocytosis, thus linking the dynamic actin cytoskeleton network with AQP2 trafficking.

DOI： 10.1242/jcs.204842

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：NATURE PUBLISHING GROUP  

Helium ion scanning microscopy (HIM) is a novel technology that directly visualizes the cell surface ultrastructure without surface coating. Despite its very high resolution, it has not been applied extensively to study biological or pathology samples. Here we report the application of this powerful technology to examine the three-dimensional ultrastructural characteristics of proteinuric glomerulopathy in mice with CD2-associated protein (CD2AP) deficiency. HIM revealed the serial alteration of glomerular features including effacement and disorganization of the slit diaphragm, followed by foot process disappearance, flattening and fusion of major processes, and eventual transformation into a podocyte sheet as the disease progressed. The number and size of the filtration slit pores decreased. Strikingly, numerous "bleb" shaped microprojections were observed extending from podocyte processes and cell body, indicating significant membrane dynamics accompanying CD2AP deficiency. Visualizing the glomerular endothelium and podocyte-endothelium interface revealed the presence of endothelial damage, and disrupted podocyte and endothelial integrity in 6 week-old Cd2ap-KO mice. We used the HIM technology to investigate at nanometer scale resolution the ultrastructural alterations of the glomerular filtration apparatus in mice lacking the critical slit diaphragm-associated protein CD2AP, highlighting the great potential of HIM to provide new insights into the biology and (patho)physiology of glomerular diseases.

DOI： 10.1038/s41598-017-08304-3

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Semaphorin3A is a secreted protein known to be involved in organogenesis, immune responses and cancer. In the kidney, semaphorin3A is expressed in the glomerular podocytes, distal tubules and collecting tubules, and believed to play a role in the regulation of the kidney development and function. We examined the serum and urinary semaphorin3A levels in 72 patients with renal disease and 5 healthy volunteers. The patients had been diagnosed with thin basement membrane disease (n=4), minimal change nephrotic syndrome (MCNS; n=22), IgA nephritis (n=21), membranous nephropathy (n=16) and focal segmental glomerular sclerosis (n=9). The level of urinary semaphorin3A in MCNS patients tended to be relatively high among all disease groups. We also investigated the urinary semaphorin3A level in 7 patients with MCNS from disease onset to remission during the drug therapy. MCNS patients in pre-remission states had higher urinary semaphorin3A levels than those in post-remission states receiving immunosuppressive therapies. These results suggested that the urinary semaphorin3A level correlates with the MCNS activity. Semaphorin3A has the potential as a biomarker for MCNS to clarify the reactivity for therapy and may be useful in examining other glomerular diseases with proteinuria as well.

DOI： 10.2147/IJNRD.S132980

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

Stem cell therapies against renal injury have been advancing. The many trials for renal regeneration are reported to be effective in many kinds of renal injury models. Regarding the therapeutic mechanism, it is believed that stem cells contribute to make regeneration via not only direct stem cell differentiation in the injured space but also indirect effect via secreted factors from stem cells. Direct differentiation from stem cells to renal composed cells has been reported. They differentiate to renal composed cells and make functions. However, regarding renal regeneration, stem cells are discussed to secrete many kinds of growth factors, cytokines, and chemokines in paracrine or autocrine manner, which protect against renal injury, too. In addition, it is reported that stem cells have the ability to communicate with nearby cells via microvesicle-related RNA and proteins. Taken together from many reports, many secreted factors from stem cells were needed for renal regeneration orchestrally with harmony. In this review, we focused on the effects and insights of stem cells and regenerative factors from stem cells.

DOI： 10.1155/2015/537204

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.bbrc.2014.03.111

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：MARY ANN LIEBERT, INC  

Long-term peritoneal dialysis (PD) causes chronic peritoneal damage. Peritoneal mesothelial cells (PMCs) play an important role in peritoneal function. We investigated the possibility of cell therapy using the PMCs to prevent peritoneal damage in PD patients. We harvested human PMCs from the PD effluent of PD patients. The PMCs were separated based on morphological characteristics into epithelial-like (Epi) cells and fibroblast-like (Fib) cells by the limiting dilution method. We transplanted these cells into nude mice whose parietal and visceral peritoneum were scratched by mechanical scraping. The transplanted cells were detected at the parietal and visceral peritoneum. Compared with the positive control, the Epi cell therapy group showed very few adhesions and exhibited no thickening of the parietal and visceral peritoneum. However, the group with Fib cell therapy could not inhibit peritoneal adhesion and thickening. In addition, hepatocyte growth factor was expressed by the grafted Epi cells but not Fib cells. Fib cells expressed vascular endothelial growth factor stronger than Epi cells. These two types of cells from the same patient showed different characteristics and effects for cell therapy. These findings suggest that the PMCs from the PD patient showed different characteristics, such as Epi cells and Fib cells, and the selection of PMCs is important for cell therapy on the point of not only the direct cellular interactions but also cytokine secretion from the grafted cells. Furthermore, the differences in the morphological cell characteristics may influence their role in peritoneal regeneration.

DOI： 10.1089/ten.TEA.2013.0130

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：OKAYAMA UNIV MED SCHOOL  

Immunosuppressive agents are generally administered to treat kidney diseases. However, it is unclear whether renal stem/progenitor cells are directly affected by the immunosuppressive agents. We used normal rat kidney cells, ureteric bud cells and rat kidney stem/progenitor cells in this study. Mizoribine (MZR), cyclophosphamide (CPA) and cyclosporine (CyA) were added to the culture media of these cells. We evaluated the effects of these immunosuppressive agents on cell proliferation using an electrical cell-substrate impedance sensing system (ECIS) and their effects on the process of renal regeneration using the ischemia-reperfusion (I/R) injury rat model. The ECIS data showed that proliferation of each of the 3 types of cells was significantly suppressed by MZR. MZR treatment enhanced renal tubular injury in ischemia-reperfusion (I/R) injured rats, and significantly decreased levels of M-phase cells and Nestin-positive cells. These results suggested that MZR inhibits the cell cycle of renal stem/progenitor cells;thus, physicians should take note that MZR might affect not only inflammation but also renal regeneration.

DOI： 10.18926/AMO/52138

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

BACKGROUND: Membranous nephropathy is one of the most common causes of nephrotic syndrome in adults. In contrast, acquired factor V inhibitor is a rare bleeding disorder. CASE PRESENTATION: A 62-year-old Asian man with a history of cerebral hemorrhage, purpura, eosinophilia and hyper immunoglobulin E syndrome developed proteinuria. The bleeding disorder was diagnosed with acquired factor V inhibitors. A renal biopsy revealed that he suffered from membranous nephropathy with glomerular endothelial damage which is reported to be involved in another factor disorder. After the steroid administration, the coagulation test and proteinuria were improved. CONCLUSIONS: The presence of factor V inhibitors may have been involved in the development of membranous nephropathy.

DOI： 10.1186/1756-0500-6-553

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

BACKGROUND: Common variable immunodeficiency (CVID) is a disorder characterized by hypogammaglobulinemia without a known predisposing cause. CASE PRESENTATION: We report a 36-year-old man who had suffered membranoproliferative glomerulonephritis (MPGN) in his childhood, later diagnosed with CVID at 35 years of age. He presented at our hospital with signs of proteinuria. A renal biopsy revealed he suffered from focal segmental glomerulosclerosis (FSGS), possibly due to obesity and hypertension, not CVID - associated MPGN. CONCLUSION: This is the first case report of FSGS in a CVID patient. In this case, we have to pay attention not only to the treatment of obesity and hypertension for FSGS but also to the recurrence of immune-complex glomerulonephritis such as MPGN, in case of the restoration of hypogammaglobulinemia.

DOI： 10.1186/1471-2369-13-46

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担当区分：筆頭著者  

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：AMER SOC NEPHROLOGY  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(株)ニュー・サイエンス社  

Sodium-glucose cotransporter 2(SGLT2)阻害剤の腎臓および心臓保護効果は複数の大規模な臨床試験の結果から明らかになっている。SGLT2阻害剤による臓器保護効果の機序は解明されていない点が多いが,その機序の一つにオートファジー不全の改善効果があることが分かってきた。SGLT2阻害剤は尿中へのグルコース排泄による絶食様状態を介してオートファジーの制御に重要であるシグナル伝達経路のmTOR,AMP活性化プロテインキナーゼ(AMPK),サーチュイン1(SIRT1),低酸素誘導因子(HIF)の制御を介してオートファジーフラックスを増強すると考えられる。特に腎臓では近位尿細管細胞と糸球体上皮細胞(ポドサイト),心臓では心筋細胞のオートファジー制御を介して保護効果がもたらされる。本稿ではSGLT2阻害剤のオートファジー制御による臓器保護作用についての知見を概観する。(著者抄録)

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記述言語：日本語   出版者・発行元：(一社)日本病態栄養学会  

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J-GLOBAL

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J-GLOBAL

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J-GLOBAL

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記述言語：日本語   出版者・発行元：(株)北隆館  

腎糸球体障害の進行課程における共通経路として糸球体上皮細胞(ポドサイト)障害が重要であるが、その病態機序は十分に解明されていない。セマフォリン3A(SEMA3A)は細胞間の情報伝達物質として機能する分泌型蛋白で、細胞増殖、遊走、免疫細胞の制御などの多彩な生理活性作用を持つ。筆者らはポドサイト障害マウスモデルにおいて、ポドサイト内のSEMA3A経路の活性化とその下流のc-Jun N-terminal Kinase(JNK)シグナルを介してアポトーシスが誘導されること、さらにその障害はSEMA3A阻害剤により抑制されることを解明し、ポドサイト障害に対するSEMA3Aをターゲットとする新たな治療法の可能性を示した。(著者抄録)

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：英語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(株)ニュー・サイエンス社  

腎糸球体障害の進行課程における共通経路として糸球体上皮細胞(ポドサイト)障害が重要であるが、ポドサイト障害に対する直接的な治療法は確立していない。セマフォリン3A(SEMA3A)は細胞間の情報伝達物質として機能する分泌型蛋白であり、細胞増殖、遊走、血管新生、免疫細胞の制御など多彩な生理活性作用を持つ。SEMA3Aはポドサイトに発現するが、その発現量増加と腎臓病進展との関連性が報告されている。筆者らは薬剤性ポドサイト障害モデルのポドサイトにおいて、SEMA3A経路の活性化とその下流のc-Jun N-terminal Kinase(JNK)シグナルを介してアポトーシスが誘導されること、さらにそれがSEMA3A阻害剤により抑制されることを解明し、ポドサイト障害に対するSEMA3Aをターゲットとする新たな治療法の可能性を示した。(著者抄録)

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

医学の進歩は著しく,腎臓疾患の概念や診断・治療も大きく変化している。医学論文数は2000年に入り急増し,増加に伴った情報氾濫が起きており,その適切な情報の取捨選択は限られた時間の中では困難である。今回われわれは医学中央雑誌に収蔵されている腎臓領域の原著論文の要約を全てテキストマイニングし解析を行った。医学中央雑誌にて検索した1982年以降に収載された「慢性腎臓病」関連の論文数(症例報告・事例を除く)は12,801報であった。それらの原著論文の要約・アブストラクトから抽出した述べ単語総数は8,556,712語であった。「慢性腎臓病」で医学中央雑誌から論文を抽出しているにも関わらず,上位に挙がった単語には「透析」,「血液透析」などが「慢性腎臓病」,「慢性腎不全」よりも出現頻度は高かった。単語のネットワーク構造解析では,「透析」,「血圧」,「慢性腎臓病」,「透析条件」などのグループが認められ,トピックとして多くの論文があることが示唆された。単語頻度推移解析では年代ごとの出現単語頻度を解析したところ,「慢性腎臓病」や慢性腎臓病の機能を評価する「eGFR」は2005年以降に多く記載されており,「慢性腎不全」は1982年以降認められていたが,2002年以降には徐々にその割合は減少しており,慢性腎不全といった腎機能低下を示す包括的な単語使用から,慢性腎臓病の提唱により単語出現頻度が移行していることが示唆された。以上より「慢性腎臓病」関連論文では透析に関係する単語が多く抽出された。一方,各糸球体性疾患の病名は多岐にわたり,反対に末期腎不全の腎代替療法は血液透析や腹膜透析に集約されている可能性も示唆された。医学中央雑誌に収載されている慢性腎臓病関連の原著論文をテキストマイニングの手法を用いて解析を行い,慢性腎臓病関連の変動を把握することが可能であった。(著者抄録)

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本臨床腎移植学会  

【研究目的】移植腎障害(KGI)を非侵襲的に診断しうるバイオマーカーの探索を尿中のエクソソーム・微小嚢胞(EV)中のmRNA解析により行った。【方法】全国11施設にて計127症例の腎移植後の尿検体を集めた。各尿検体からEV RNAを抽出し定量RT-PCR測定を行い、腎生検を含む病理診断結果と比較しマーカー候補遺伝子ならびに複数候補による診断式の診断性能を検証した。【結果】拒絶反応の鑑別では以前に報告したANXA1の上昇は確認できなかったものの、CXCL9、CXCL10、UMODがT細胞性拒絶反応(TCMR)で上昇し、慢性抗体関連拒絶反応(cABMR)ではSPNS2の上昇が確認された。Sparse Logistic Regression(SLR)解析による複数マーカー候補からなる診断式にてcABMRと他のKGI群とAUC 0.875で鑑別できることを確認した。また、臨床上判定が困難な慢性カルシニューリン毒性に比しても、SLR解析でAUC 0.886でcABMRの鑑別が可能であった。加えて、間質線維化・尿細管萎縮や慢性腎障害重症度と相関がみられるマーカー候補POTEMを確認し、SLR解析による診断式でおのおのAUC 0.830、0.850で鑑別できた。【結論】尿中EV RNA解析によりKGIを非侵襲的に診断できうる可能性を示した。(著者抄録)

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その他リンク： https://search-tp.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2020&ichushi_jid=J06262&link_issn=&doc_id=20200806480025&doc_link_id=%2Fem0sfcrt%2F2020%2F000801%2F025%2F0142-0150%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fem0sfcrt%2F2020%2F000801%2F025%2F0142-0150%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(株)医学書院  

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その他リンク： https://search-tp.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2020&ichushi_jid=J06514&link_issn=&doc_id=20200612230029&doc_link_id=10.11477%2Fmf.1429202653&url=https%3A%2F%2Fdoi.org%2F10.11477%2Fmf.1429202653&type=%88%E3%8F%91.jp_%83I%81%5B%83%8B%83A%83N%83Z%83X&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00024_2.gif

記述言語：日本語   出版者・発行元：(株)医学書院  

＜文献概要＞慢性腎臓病(chronic kidney disease:以下、CKD)の進行に伴って、腎予備能が低下するとともに、高血圧、浮腫、高カリウム血症、貧血など、さまざまな異常所見が顕在化しやすく、それぞれが腎障害の加速因子となりうる。腎予後の改善や心血管合併症予防の観点から、臨床所見や検査データを基に、それぞれの病態に対して食事療法や薬剤療法を行い、厳格に管理する。

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その他リンク： https://search-tp.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2020&ichushi_jid=J06514&link_issn=&doc_id=20200612230027&doc_link_id=10.11477%2Fmf.1429202651&url=https%3A%2F%2Fdoi.org%2F10.11477%2Fmf.1429202651&type=%88%E3%8F%91.jp_%83I%81%5B%83%8B%83A%83N%83Z%83X&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00024_2.gif

記述言語：日本語   出版者・発行元：(社医財)大樹会総合病院回生病院  

静脈性血管瘤(VA)は無症状で経過することが多く、炎症や血栓を形成して血栓性静脈炎や致命的な肺塞栓症(PE)をきたす事があり、注目されている。2011年1月〜2019年3月に当院で経験した静脈性血管瘤(VA)症例を対象に、臨床的検討を行った。当院で経験したVA症例は8例で、男性は3例、女性は5例であった。年齢は39〜84歳で、平均59歳であった。発生部位は、上肢3例、腹壁1例、下肢4例であった。病型分類は2種類あり、嚢胞状タイプ(Sacciform)がI型、紡錘状タイプ(Fusiform)がII型である。I型が4例、II型が4例であった。全例手術治療を行い、全摘出術を行った。術後経過も全例順調で特別問題となった症例はなかった。本稿では、84歳女性、49歳男性、47歳女性の症例について詳述した。

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記述言語：日本語   出版者・発行元：(一社)日本内科学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内科学会  

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記述言語：日本語   出版者・発行元：(一社)日本透析医学会  

J-GLOBAL

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記述言語：日本語   出版者・発行元：(一社)日本透析医学会  

J-GLOBAL

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記述言語：日本語   出版者・発行元：(一社)日本透析医学会  

J-GLOBAL

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：(一社)日本腎臓学会  

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記述言語：日本語   出版者・発行元：東京医学社  

CiNii Article

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その他リンク： http://search.jamas.or.jp/link/ui/2009346568

開催年月日： 2021年6月

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記述言語：日本語  

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記述言語：日本語  

静脈性血管瘤(VA)は無症状で経過することが多く、炎症や血栓を形成して血栓性静脈炎や致命的な肺塞栓症(PE)をきたす事があり、注目されている。2011年1月〜2019年3月に当院で経験した静脈性血管瘤(VA)症例を対象に、臨床的検討を行った。当院で経験したVA症例は8例で、男性は3例、女性は5例であった。年齢は39〜84歳で、平均59歳であった。発生部位は、上肢3例、腹壁1例、下肢4例であった。病型分類は2種類あり、嚢胞状タイプ(Sacciform)がI型、紡錘状タイプ(Fusiform)がII型である。I型が4例、II型が4例であった。全例手術治療を行い、全摘出術を行った。術後経過も全例順調で特別問題となった症例はなかった。本稿では、84歳女性、49歳男性、47歳女性の症例について詳述した。

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開催年月日： 2020年2月

記述言語：日本語  

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記述言語：日本語  

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その他リンク： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2020&ichushi_jid=J06514&link_issn=&doc_id=20200612230029&doc_link_id=10.11477%2Fmf.1429202653&url=https%3A%2F%2Fdoi.org%2F10.11477%2Fmf.1429202653&type=%88%E3%8F%91.jp_%83I%81%5B%83%8B%83A%83N%83Z%83X&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00024_2.gif

開催年月日： 2020年

記述言語：日本語  

＜文献概要＞慢性腎臓病(chronic kidney disease:以下、CKD)の進行に伴って、腎予備能が低下するとともに、高血圧、浮腫、高カリウム血症、貧血など、さまざまな異常所見が顕在化しやすく、それぞれが腎障害の加速因子となりうる。腎予後の改善や心血管合併症予防の観点から、臨床所見や検査データを基に、それぞれの病態に対して食事療法や薬剤療法を行い、厳格に管理する。

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その他リンク： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2020&ichushi_jid=J06514&link_issn=&doc_id=20200612230027&doc_link_id=10.11477%2Fmf.1429202651&url=https%3A%2F%2Fdoi.org%2F10.11477%2Fmf.1429202651&type=%88%E3%8F%91.jp_%83I%81%5B%83%8B%83A%83N%83Z%83X&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00024_2.gif

開催年月日： 2020年

記述言語：日本語  

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開催年月日： 2020年

記述言語：日本語  

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開催年月日： 2020年

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開催年月日： 2019年5月

記述言語：日本語  

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開催年月日： 2019年5月

記述言語：日本語  

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開催年月日： 2019年5月

記述言語：日本語  

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開催年月日： 2019年5月

記述言語：日本語  

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開催年月日： 2019年

記述言語：日本語  

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開催年月日： 2019年

記述言語：日本語  

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開催年月日： 2019年

記述言語：日本語  

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開催年月日： 2019年

記述言語：日本語  

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開催年月日： 2019年

記述言語：日本語  

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開催年月日： 2019年

記述言語：日本語  

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開催年月日： 2014年5月

記述言語：英語  

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開催年月日： 2014年

記述言語：日本語  

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開催年月日： 2014年

記述言語：日本語  

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開催年月日： 2014年

記述言語：日本語  

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開催年月日： 2013年2月28日

記述言語：日本語  

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開催年月日： 2013年2月

記述言語：日本語  

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開催年月日： 2013年

記述言語：日本語  

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開催年月日： 2013年

記述言語：日本語  

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開催年月日： 2013年

記述言語：日本語  

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開催年月日： 2013年

記述言語：日本語  

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開催年月日： 2013年

記述言語：日本語  

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開催年月日： 2013年

記述言語：日本語  

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開催年月日： 2012年

記述言語：日本語  

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開催年月日： 2012年

記述言語：日本語  

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開催年月日： 2012年

記述言語：日本語  

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開催年月日： 2012年

記述言語：日本語  

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開催年月日： 2011年

記述言語：日本語  

59歳男。糖尿病性腎症のため腹膜透析で維持透析を受けていた。今回、発熱、感冒症状の後に意識レベル低下、痙攣発作が出現し、頭部CTで異常なく、髄液検査よりヘルペス脳炎を疑い治療を開始した。しかし、発熱、痙攣発作、四肢不随意運動が持続して当院救急入院となった。脳波、髄液、血液検査所見より無菌性脊髄炎、単純ヘルペス脳炎と診断し、アシクロビル、メロペン、献血ベニロン、グリセオールおよび免疫グロブリンの治療を開始した。また、腹膜透析は継続しながら、溢水管理のため週1回の血液透析を併用した。治療後、単核球優位の細胞数増多を認めた髄液所見は改善し、痙攣発作も消失したが、意識障害、四肢不随意運動は持続し、急性散在性脳脊髄炎を疑いMRIを施行したところ、大脳白質、右優位に線条体に散在性の高信号域を認め診断を確定した。ステロイドパルス療法(ソル・メドロール1000mg/日×3)を開始したところ、急速に意識レベルは改善して四肢不随意運動も消失し、その後のMRIでは高信号域の軽減を認めた。全身状態が安定した後、近医転院となった。

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開催年月日： 2011年

記述言語：日本語  

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開催年月日： 2010年

記述言語：日本語  

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開催年月日： 2010年

記述言語：日本語  

83歳男。患者は咳嗽があり近医を受診、間質性肺炎と診断され、ブレドニゾロンの内服が開始されたものの、発熱および倦怠感が出現し、あわせて尿異常、炎症反応高値、MPO(myeloperoxidase)-ANCA(anti-neutrophil cytoplasmic autoantibody)陽性が認められた。今回、腎生検目的にて、著者らの施設へ紹介となったが、所見では糸球体に半月体形成はみられなかったものの、小葉間動脈に血管炎像が認められ、MPA(microscopic polyangitis)と診断された。治療はステロイドパルス療法施行後にプレドニゾロンの投与が開始されたが、血管炎腎外病変である間質性肺炎の増悪と感染のコントロールに難渋し、最終的に呼吸状態の悪化により患者は死亡となった。尚、剖検所見では腎臓は小葉間動脈領域の内膜の断裂、線維性肥厚狭小化がみられたが、血管周囲のリンパ節浸潤は軽度であり、血管炎瘢痕期と考えられた。また、死因としては間質性肺炎の増悪が推察された。

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開催年月日： 2010年

記述言語：日本語  

著者らは腎生検を施行した70歳以上の高齢者ネフローゼ症候群33症例(平均年齢74歳)を対象に病理学的検討を行った。1)続発性ネフローゼ症候群34%と他施設の報告とには差はなかったが、一方で原発性ネフローゼ症候群は膜性腎症の割合が多いとされているものの、微小変化群が多く認められた。また、腎機能低下症例も多く認められた。2)20例でステロイド治療が開始され、4例でネフローゼ症候群から溢水によって心不全を呈し一時的に透析を必要とした。3)治療開始から3ヵ月後に52%、1年後に73%の症例に治療効果が確認され、高齢者であっても積極的な治療によって腎予後、生命予後の改善が期待できる症例の存在が示唆された。4)死亡は経過中8例にみられ、最終的に透析導入となった腎死症例が4例に認められた。5)腎生検による重大な合併症は認められなかったが、ネフローゼ症候群による合併症は感染症を7例、深部静脈血栓症を1例、出血性潰瘍を2例に確認された。尚、悪性腫瘍の合併は10例に認められた。

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開催年月日： 2010年

記述言語：日本語  

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開催年月日： 2009年8月25日

記述言語：日本語  

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開催年月日： 2009年8月25日

記述言語：日本語  

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開催年月日： 2009年5月25日

記述言語：日本語  

60歳女。1年4ヵ月前にCAPD導入し、APDにて維持していた。今回、微熱と汎血球減少症が出現し、結核性骨髄炎と診断して治療中、副腎結核による副腎不全を合併した。抗結核薬4剤による治療とヒドロコルチゾンの内服により結核、副腎不全とも改善したが、抗結核薬の副作用が強く治療に難渋した。また副腎結核の診断では組織検査で結核菌が検出されず、悪性腫瘍との鑑別のために副腎摘出を要した。

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開催年月日： 2009年5月8日

記述言語：日本語  

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開催年月日： 2009年

記述言語：日本語  

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開催年月日： 2009年

記述言語：日本語  

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開催年月日： 2008年8月25日

記述言語：日本語  

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開催年月日： 2008年3月20日

記述言語：日本語  

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開催年月日： 2008年

記述言語：日本語  

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開催年月日： 2008年

記述言語：日本語  

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