論文 - 大橋　俊孝

Biochemical investigations on prolidase and prolinase in erythrocytes from patients with prolidase deficiency. 査読 国際誌

H Kodama, H Mikasa, T Ohhashi, T Ohno, J Arata

Clinica chimica acta; international journal of clinical chemistry   173 ( 3 )   317 - 23   1988年4月

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

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Biochemical studies on prolidase in sera from control, patients with prolidase deficiency and their mother. 査読 国際誌

T Ohhashi, T Ohno, J Arata, H Kodama

Journal of inherited metabolic disease   11 ( 2 )   166 - 73   1988年

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Prolidase activity in serum from normal subjects and the mother of two patients was readily detected without adding Mn2+ to the assay, and the activity was increased by addition of Mn2+ to the assay or preincubation with Mn2+. However, the activity in serum from patients with prolidase deficiency against gly-pro, leu-pro and val-pro could not be detected irrespective of Mn2+ conditions and activity against met-pro, ala-pro and phe-pro also showed a marked reduction compared to controls. Both normal and the patients' mother's prolidase activity against gly-pro was reduced about 20% at 60 degrees C compared to the activity at 37 degrees C, but the addition of Mn2+ at 55 degrees C increased the activity about 1.8-fold, whereas prolidase activity of patients could not be increased by the addition of Mn2+. The addition of Co2+ increased prolidase activity in serum from control and the patients' mother but did not increase the heat stability. These results indicate that prolidase in serum from patients with prolidase deficiency is altered rather than markedly reduced in amount.

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