Faculty Profiles - MA Jian Feng

Papers - MA Jian Feng

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Characterization of phytosiderophore secretion under Fe deficiency stress in Festuca rubra

JF Ma, H Ueno, D Ueno, AD Rombola, T Iwashita

PLANT AND SOIL 256 ( 1 ) 131 - 137 2003.9

Language：English Publishing type：Research paper (scientific journal) Publisher：KLUWER ACADEMIC PUBL

In the present study we investigated the response to iron (Fe) deficiency in two cultivars of Festuca rubra L. (Rubina and Barnica) used in correction of chlorosis of fruit trees cultivated on calcareous soils. We found that a Fe-chelating compound, identified as 2'-deoxymugineic acid (DMA), was secreted from the roots in response to Fe-deficiency in both cultivars. The amount of DMA secreted into solution increased with the development of Fe-deficiency. The secretion showed a distinct diurnal rhythm characterized by a secretion peak at between 2 and 5 hours after sunrise at 20degreesC. However, this secretion peak was delayed by 3 hour at low temperature (< 10&DEG;C) and occurred 3 h earlier at high temperature (30&DEG;C). When water used for the collection of root exudates was pre-warmed (25&DEG;C) or pre-cooled (10&DEG;C), this led to an earlier or a delayed secretion compared to control (15&DEG;C) under the same air temperature, respectively. Short-term shading treatment did not affect the secretion pattern of DMA. These results demonstrate that the secretion time of DMA from the roots is, at least partly controlled by the temperature in the root environment. Overall, these findings suggest that the ability of Festuca rubra to prevent Fe chlorosis symptoms('re-greening effect') of associated fruit trees is partially related to the secretion of DMA which increase Fe availability in calcareous soils.

DOI： 10.1023/A:1026285813248

Differential Al resistance and citrate secretion in barley (Hordeum vulgare L.). International journal

Zhuqing Zhao, Jian Feng Ma, Kazuhiro Sato, Kazuyoshi Takeda

Planta 217 ( 5 ) 794 - 800 2003.9

Language：English Publishing type：Research paper (scientific journal)

While barley ( Hordeum vulgare L.) is the most sensitive species to Al toxicity among small-grain crops, variation in Al resistance between cultivars does exist. We examined the mechanism responsible for differential Al resistance in 21 barley varieties. Citrate was secreted from the roots in response to Al stress. A positive correlation between citrate secretion and Al resistance [(root elongation with Al)/(root elongation without Al)] and a negative correlation between citrate secretion and Al content of root apices, were obtained, suggesting that citrate secretion from the root apices plays an important role in excluding Al and thereby detoxifying Al. The Al-induced secretion of citrate was characterized using an Al-resistant variety (Sigurdkorn) and an Al-sensitive variety (Kearney). In Sigurdkorn, Al-induced secretion of citrate occurred within 20 min, and the secretion did not increase with increasing external Al concentration. The Al-induced citrate secretion ceased at low temperature (6 degrees C) and was inhibited by anion-channel inhibitors. Internal citrate content of root apices was increased by Al exposure in Sigurdkorn, but was not affected in Kearney. The activity of citrate synthase was unaffected by Al in both Al-resistant and Al-sensitive varieties. The secretion rate of organic acid anions from barley was the lowest among wheat, rye and triticale.

DOI： 10.1007/s00425-003-1043-2

Al-induced efflux of organic acid anions is poorly associated with internal organic acid metabolism in triticale roots

JE Hayes, JF Ma

JOURNAL OF EXPERIMENTAL BOTANY 54 ( 388 ) 1753 - 1759 2003.7

Language：English Publishing type：Research paper (scientific journal) Publisher：OXFORD UNIV PRESS

The secretion of organic acid anions from roots has been identified as a mechanism of resistance to Al. However, the process leading to the secretion of organic acid anions is poorly understood. The effect of Al on organic acid metabolism was investigated in two lines of triticale (xTriticosecale Wittmark) differing in Al-induced secretion of malate and citrate and in Al resistance. The site of Al-induced secretion of citrate and malate from a resistant line was localized to the root apices (terminal 5 mm). The levels of citrate (root apices and mature root segments) and malate (mature segments only) in roots increased during exposure to Al, but similar changes were observed in both triticale genotypes. The in vitro activities of four enzymes involved in malate and citrate metabolism (citrate synthase, phosphoenolpyruvate carboxylase, malate dehydrogenase, and NADP-isocitrate dehydrogenase) were similar for sensitive and resistant lines in both root apices and mature root segments. The response of these enzymes to pH did not differ between tolerant and sensitive lines or in the presence and absence of Al. Moreover, cytoplasmic and vacuolar pH were not affected by exposure to Al in either line. Together, these results indicate that the Al-dependent efflux of organic acid anions from the roots of triticale is not regulated by their internal levels in the roots or by the capacity of the root cells to synthesize malate and citrate.

DOI： 10.1093/jxb/erg188

Characterization of silicon uptake by rice roots

K Tamai, JF Ma

NEW PHYTOLOGIST 158 ( 3 ) 431 - 436 2003.6

Language：English Publishing type：Research paper (scientific journal) Publisher：BLACKWELL PUBLISHING LTD

Rice (Oryza sativa) is a typical Si-accumulating plant and it has been suggested that it has a specific uptake system for silicic acid in the roots.
Here, we characterized this specific system in rice roots. The ability of rice roots to take up Si was much higher than that of other gramineous species.
A kinetic study indicated that Si uptake was mediated by a type of proteinaceous transporter; the K-m value was estimated to be 0.32 mm, suggesting that the transporter had a low affinity for silicic acid. Si uptake increased linearly with time, but pretreatment with Si did not affect the uptake of Si, suggesting that the system for Si uptake was not inducible. Mercuric chloride and phloretin, significantly inhibited Si uptake, but 4,4'-diisothiocyanato-stilbene-2,2'-disulfonic acid (DIDS) did not. Mercuric chloride and phloretin also inhibited water uptake, but to a lesser extent. Si uptake was unaffected by the presence of boric acid.
Taken together, the data indicate that the uptake of Si by rice roots is a transporter-mediated process and this transporter contains Cys residues but not Lys residues.

DOI： 10.1046/j.1469-8137.2003.00773.x

Genotypic variation in silicon concentration of barley grain

JF Ma, A Higashitani, K Sato, K Takeda

PLANT AND SOIL 249 ( 2 ) 383 - 387 2003.2

Language：English Publishing type：Research paper (scientific journal) Publisher：KLUWER ACADEMIC PUBL

Soluble silicon (Si) in foods and drinks has been suggested to have a protective effect against neurotoxicity of Al. We investigated the genotypic variation in Si concentration of barley grain, which has many uses including in livestock feeds, malts for beer and whisky, and some foods for human consumption. Two collections of barley, grown in the same field, were subjected to analysis; 274 standard varieties selected at the Barley Germplasm Center of the Research Institute for Bioresources, Okayama University (SV), and 135 varieties from the Barley Core Collection of Americans (BCCAM). The Si concentration of barley grain showed large variation, ranging from 0 (under detection) to 3600 mg kg(-1) in SV and from 0 to 3800 mg kg(-1) in BCCAM barleys. The Si concentration was much lower in hull-less barley than in hulled barley. The Si concentration of two-row barley was similar to that of six-row barley, suggesting that Si concentration is not affected by the number of spike rows. Si concentration also did not differ with the origin of the barley variety. More than 80% of total Si was localized in the hull. The Si concentration of the hull was between 15 343 and 27 089 mg kg(-1) in the varieties tested. A close correlation was obtained between the Si concentrations of barley grains harvested in different years, suggesting that the variation in Si concentration of barley grain is controlled genetically. These results provide fundamental data for breeding Si-rich cultivars.

DOI： 10.1023/A:1022842421926

A rice mutant defective in Si uptake

JF Ma, K Tamai, M Ichii, GF Wu

PLANT PHYSIOLOGY 130 ( 4 ) 2111 - 2117 2002.12

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC PLANT BIOLOGISTS

Rice (Oryza sativa) accumulates silicon (Si) in the tops to levels up to 10.0% of shoot dry weight, but the mechanism responsible for high Si uptake by rice roots is not understood. We isolated a rice mutant (GR1) that is defective in active Si uptake by screening M-2 seeds (64,000) of rice cv Oochikara that were treated with 10(-3) M sodium azide for 6 h at 25degreesC. There were no phenotypic differences between wild type (WT) and GR1 except that the leaf blade of GR1 remained droopy when Si was supplied. Uptake experiments showed that Si uptake by GR1 was significantly lower than that by WT at both low and high Si concentrations. However, there was no difference in the uptake of other nutrients such as phosphorus and potassium. Si concentration in the xylem sap of WT was 33-fold that of the external solution, but that of GR1 was 3-fold higher than the external solution at 0.15 mm Si. Si uptake by WT was inhibited by metabolic inhibitors including NaCN and 2,4-dinitrophenol and by low temperature, whereas Si uptake by GR1 was not inhibited by these agents. These results suggest that an active transport system for Si uptake is disrupted in GR1. Analysis of F-2 populations between GR1 and WT showed that roots with high Si uptake and roots with low Si uptake segregated at a 3:1 ratio, suggesting that GR1 is a recessive mutant of Si uptake.

DOI： 10.1104/pp.010348

Compartmentation of aluminium in leaves of an Al-accumulator, Fagopyrum esculentum Moench.

RF Shen, JF Ma, M Kyo, T Iwashita

PLANTA 215 ( 3 ) 394 - 398 2002.7

Language：English Publishing type：Research paper (scientific journal) Publisher：SPRINGER

Buckwheat (Fagopyrum esculentum Moench.) is an Al-accumulating plant, but the internal mechanism(s) of detoxification of Al is not fully understood. We investigated the subcellular localization of Al in the leaves of this plant (cv. Jianxi) by directly isolating protoplasts and vacuoles. Pure protoplasts and vacuoles from the leaves of buckwheat, grown hydroponically in Al solution, were obtained based oil light-microscopic observation and the activities of marker enzymes of cytosol and vacuoles. More than 80% of total Al in the leaves was present in the protoplasts. and was identified as an Al-oxalate complex (1:3 ratio) by 27 Al-nuclear magnetic resonance. Oxalate and Al in the protoplasts was localized in the vacuoles. These results suggest that internal detoxification of Al in the buckwheat leaves is achieved by both complexation with oxalate and sequestration into vacuoles.

DOI： 10.1007/s00425-002-0763-z

Response of rice to Al stress and identification of quantitative trait loci for Al tolerance

JF Ma, RF Shen, ZQ Zhao, M Wissuwa, Y Takeuchi, T Ebitani, M Yano

PLANT AND CELL PHYSIOLOGY 43 ( 6 ) 652 - 659 2002.6

Language：English Publishing type：Research paper (scientific journal) Publisher：OXFORD UNIV PRESS

Rice (Oryza sativa L.) shows the highest tolerance to Al toxicity among small-grain cereal crops, however, the mechanisms and genetics responsible for its high Al tolerance are not yet well understood. We investigated the response of rice to Al stress using the japonica variety Koshihikari in comparison to the indica variety Kasalath. Koshihikari showed higher tolerance at various Al concentrations than Kasalath. The Al content in root apexes was less in Koshihikari than in Kasalath, suggesting that exclusion mechanisms rather than internal detoxification are acting in Koshihikari. Al-induced secretion of citrate was observed in both Koshihikari and Kasalath, however, it is unlikely to be the mechanism for Al tolerance because there was no significant difference in the amount of citrate secreted between Koshihikari and Kasalath. Quantitative trait loci (QTLs) for Al tolerance were mapped in a population of 183 backcross inbred lines (BILs) derived from a cross of Koshihikari and Kasalath. Three putative QTLs controlling Al tolerance were detected on chromosomes 1, 2 and 6. Kasalath QTL alleles on chromosome 1 and 2 reduced Al tolerance but increased tolerance on chromosome 6. The three QTLs explained about 27% of the phenotypic variation in Al tolerance. The existence of QTLs for Al tolerance was confirmed in substitution lines for corresponding chromosomal segments.

DOI： 10.1093/pcp/pcf081

Role of root hairs and lateral roots in silicon uptake by rice

JF Ma, S Goto, K Tamai, M Ichii

PLANT PHYSIOLOGY 127 ( 4 ) 1773 - 1780 2001.12

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC PLANT BIOLOGISTS

The rice plant (Oryza sativa L. cv Oochikara) is known to be a Si accumulator, but the mechanism responsible for the high uptake of Si by the roots is not well understood. We investigated the role of root hairs and lateral roots in the Si uptake using two mutants of rice, one defective in the formation of root hairs (RH2) and another in that of lateral roots (RM109). Uptake experiments with nutrient solution during both a short term (up to 12 h) and relatively long term (26 d) showed that there was no significant difference in Si uptake between RH2 and the wild type (WT), whereas the Si uptake of RM109 was much less than that of WT. The number of silica bodies formed on the third leaf in RH2 was similar to that in WT, but the number of silica bodies in RM109 was only 40% of that in WT, when grown in soil amended with Si under flooded conditions. There was also no difference in the shoot Si concentration between WT and RH2 when grown in soil under upland conditions. Using a multi-compartment transport box, the Si uptake at the root tip (0-1 cm, without lateral roots and root hairs) was found to be similar in WT, RH2, and RM109. However, the Si uptake in the mature zone (1-4 cm from root tip) was significantly lower in RM109 than in WT, whereas no difference was found in Si uptake between WT and RH2. All these results clearly indicate that lateral roots contribute to the Si uptake in rice plant, whereas root hairs do not. Analysis of F-2 populations between RM109 and WT showed that Si uptake was correlated with the presence of lateral roots and that the gene controlling formation of lateral roots and Si uptake is a dominant gene.

DOI： 10.1104/pp.127.4.1773

Distribution and mobility of aluminium in an Al-accumulating plant, Fagopyrum esculentum Moench.

RF Shen, JF Ma

JOURNAL OF EXPERIMENTAL BOTANY 52 ( 361 ) 1683 - 1687 2001.8

Language：English Publishing type：Research paper (scientific journal) Publisher：OXFORD UNIV PRESS

Buckwheat (Fagopyrum esculentum Moench. cv. Jianxi) accumulates high concentrations of Al in the leaves without showing any toxicity. To understand the accumulation mechanism of All in buckwheat, the distribution and mobility of Al in buckwheat were investigated. Relatively long-term treatment (28 d) with Al led to a decrease in Al concentration from old to young leaves, while a short-term (1 d) exposure to Al resulted in a uniform distribution of All in the leaves. When the fourth leaf was wrapped inside a transparent plastic bag to suppress transpiration, the Al concentration of this leaf was only one-quarter of that in the corresponding leaf without wrappng. Wit in a leaf, the Al concentration at the margins was much higher than that in the centre. These results indicate that Al distribution in the leaves is controlled by both rate and duration of transpiration. The mobility of Al between old and new leaves was studied by first growing plants in a solution with Al, followed by culture in a solution without Al. The Al content in the two new leaves appeared after removal of external Al was very low, whereas that in the old leaves did not decrease but continued to increase. The increased Al content was found to be translocated from Al remaining in the roots. It is concluded that Al is not mobile once it is accumulated in the leaf.

DOI： 10.1093/jexbot/52.361.1683

Aluminium tolerance in plants and the complexing role of organic acids

JF Ma, PR Ryan, E Delhaize

TRENDS IN PLANT SCIENCE 6 ( 6 ) 273 - 278 2001.6

Language：English Publisher：ELSEVIER SCIENCE LONDON

The aluminium cation Al3+ is toxic to many plants at micromolar concentrations. A range of plant species has evolved mechanisms that enable them to grow on acid soils where toxic concentrations of Al3+ can limit plant growth, Organ ic acids play a central role in these aluminium tolerance mechanisms. Some plants detoxify aluminium in the rhizosphere by releasing organic acids that chelate aluminium. In at least two species, wheat and maize, the transport of organic acid anions out of the root cells is mediated by aluminium-activated anion channels in the plasma membrane. Other plants, including species that accumulate aluminium in their leaves, detoxify aluminium internally by forming complexes with organic acids.

DOI： 10.1016/S1360-1385(01)01961-6

Regulatory mechanisms of Al-induced secretion of organic acids anions-Involvement of ABA in the Al-induced secretion of oxalate in buckwheat

Ma, J. F, Zhang, W, Zhao, Z

Plant Nutrition-Food security and sustainability of agro-ecosystems through basic and applied Research. 2001

Pattern of aluminum-induced secretion of organic acids differs between rye and wheat

XF Li, JF Ma, H Matsumoto

PLANT PHYSIOLOGY 123 ( 4 ) 1537 - 1543 2000.8

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC PLANT PHYSIOLOGISTS

Al-Induced secretion of organic acids from the roots has been considered as a mechanism of Al tolerance, but the processes leading to the secretion of organic acids are still unknown. In this study, the secretion pattern and alteration in the metabolism of organic acids under Al stress were examined in rye (Secale cereale L. cv King) and wheat (Triticum aestivum L. cv Atlas 66). Al induced rapid secretion of malate in the wheat, but a lag (6 and 10 h for melic and citric acids, respectively) between the exposure to Al and the secretion of organic acids was observed in the rye. The activities of isocitrate dehydrogenase, phosphoenolpyruvate carboxylase, and malate dehydrogenase were not affected by Al in either plant. The activity of citrate synthase was increased by the exposure to Al in the rye, but not in the wheat. The secretion of malate was not suppressed at low temperature in the wheat, but that of citrate was stopped in the rye. The Al-induced secretion of citrate from roots of the rye was inhibited by the inhibitors of a citrate carrier, which transports citrate from the mitochondria to the cytoplasm. All of these results suggest that alteration in the metabolism of organic acids is involved in the Al-induced secretion of organic acids in rye, but only activation of an anion channel seems to be responsible for the rapid secretion of malate in the wheat.

DOI： 10.1104/pp.123.4.1537

Role of organic acids in detoxification of aluminum in higher plants

JF Ma

PLANT AND CELL PHYSIOLOGY 41 ( 4 ) 383 - 390 2000.4

Language：English Publisher：OXFORD UNIV PRESS

Phytotoxicity of aluminum ion (Al3+) is a serious problem limiting crop production on acid soils. Organic acids with Al-chelating ability play an important role in the detoxification of Al both externally and internally. Al is detoxified externally by the secretion of organic acids such as citric, oxalic, and /or malic acids from the roots. The secretion of organic acids is highly specific to Al and the site of secretion is localized to the root apex. The kind of organic acids secreted as well as secretion pattern differ among plant species. There are two patterns of Al-induced secretion of organic acids: In pattern I, there is no discernible delay between the addition of Al and the onset of the release of organic acids. Activation of the anion channel seems to be involved in this pattern; In pattern II, there is a marked lag phase between the addition of Al and the onset of organic acid release. The action of genes related to the metabolism and secretion of organic acids seems to be involved in this pattern. Internal detoxification of Al in Al-accumulating plants is achieved by the formation of Al-organic acid complex. For instance, the complex of Al-citrate (1:1) in hydrangea and Al-oxalate (1:3) in buckwheat has been identified.

DOI： 10.1093/pcp/41.4.383

IDENTIFICATION OF CHROMOSOMAL ARM LOCATION OF AL-TOLERANT GENES IN RYE

MA Jian Feng, TAKETA Shin, YANG Zhen Ming, TAKEDA Kazuyoshi, MATSUMOTO Hideaki

40 s101 - s101 1999.3

IDENTIFICATION OF CHROMOSOMAL ARM LOCATION OF GENES CONTROLLING HYDROXYLATION OF PHYTOSIDEROPHORES IN BARLEY

MA Jian Feng, TAKETA Shin, CHANG Yi-Chieh, NOMOTO Kyosuke, IWASHITA Takashi, TAKEDA Kazuyoshi, MATSUMOTO Hideaki

40 s96 - s96 1999.3

Role of metal-chelating substances in root exudates

MA Jian Feng

Root Research 7 ( 4 ) 105 - 108 1998.12

9-21 ソバにおけるアルミニウムストレスの抵抗性機構 : アルミニウム存在形態の同定(9.植物の無機栄養)

馬建鋒, 平舘俊太郎, 松本英明

日本土壌肥料学会講演要旨集 44 75 - 75 1998

Early events induced by aluminum stress in elongating cells of wheat root

Masao Sasaki, Yoko Yamamoto, Jian Feng Ma, Hideaki Matsumoto

Soil Science and Plant Nutrition 43 ( SPEC. ISS. ) 1009 - 1014 1997.12

Publishing type：Research paper (international conference proceedings)

The relation between the toxic effects of alminum (Al) on growth and cell viability in roots of wheat (Triticum aestivum L. cv Atlas 66) was investigated. Root elongation of wheat was reduced after 3 h of treatment with 50 pM AlCl3 at pH 4.7. Only the apical region of the root treated with Al was stained with hematoxylin. Al treatment decreased the length and increased the diameter of the cells at the elongation zone of the root. Staining with propidium iodide which can permeate through only a damaged plasma membrane showed that treatment with Al for longer than 3 h decreased cell viability. These findings suggest that inhibition of cell elongation by Al is partially accompanied by cell death. Lignin was deposited in the cell wall of the second and third layers of the cortex in the elongation zone of the root. Also the disruption of microtubules ocurred in elongating cells treated with 50 μM Al for 3 h. The effects of Al stress on root elongation and cell viability in the elongation zone were inhibited by 1 mM Ca. In conclusion, inhibition of root elongation by Al is strongly related to the inhibition of cell elongation, accompanied by decreased cell viability, lignin deposition and the disruption of microtubules.

DOI： 10.1080/00380768.1997.11863708

A rapid hydroponic screening for aluminium tolerance in barley

JF Ma, SJ Zheng, XF Li, K Takeda, H Matsumoto

PLANT AND SOIL 191 ( 1 ) 133 - 137 1997.4

Language：English Publishing type：Research paper (scientific journal) Publisher：SPRINGER

Selection and breeding of crops for aluminium (Al) tolerance is a useful approach to increase production on acid soils. This requires a rapid and reliable system to discriminate between Al-tolerant and Al-sensitive genotypes. A hydroponic system was developed to screen for Al tolerance in barley (Hordeum vulgare L.) to overcome several problems encountered in previous screening methods. Four levels of Al (5, 10, 20, and 40 mu M) in 1 mM CaCl2 solution at pH 4.5 were used to rank lines for Al-tolerance. Each line was cultured in a different compartment to eliminate chemical and pH interactions among lines. To avoid changes in Al tolerance due to other factors such as the calcium (Ca) concentration of the solution, Al-tolerant (Atlas 66) and Al-sensitive (Scout 66) cultivars of wheat (Triticum aestivum L.) were used as reference cultivars. Five ranks of Al tolerance from highly tolerant to highly sensitive were established by comparison with each reference. Eriochrome cyanine R staining was used for the rapid evaluation of Al tolerance. This screening system allowed classification of about 50 barley lines into five different Al tolerance groups within one week. Using this system, screening of ca. 600 barley lines from various regions of the world was conducted. Most lines were sensitive to Al, but ninety lines showed intermediate Al-tolerance. Thirty nine lines were highly sensitive to Al in solution.

DOI： 10.1023/A:1004257711952

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