2022/10/01 更新

写真a

タカシバ ショウゴ
髙柴 正悟
TAKASHIBA Shogo
所属
医歯薬学域 教授
職名
教授
連絡先
メールアドレス
プロフィール
歯や口の健康だけではなく全身の健康にいろいろと関係する「歯周病」の研究を,細菌学,免疫学,分子細胞生物学の観点によって,基礎から臨床まで幅広く行っています。 特に,感染の制御,炎症の制御,そして組織再生の制御の3分野での視点でもって,研究を発展させています。 最近では,臨床現場の先生方と連携した臨床疫学・観察・介入の研究へと発展させ,さらには種々の製品開発に関わる研究へと視野を拡大しています。

学位

  • 歯学博士 ( 1990年3月   岡山大学 )

  • 歯学士 ( 1986年3月   岡山大学 )

研究キーワード

  • 歯周病学

  • 歯内療法学

  • Periodontal Science (Periodontology & Endodontology)

  • 歯学

研究分野

  • ライフサイエンス / 保存治療系歯学

  • ライフサイエンス / 保存治療系歯学

学歴

  • 岡山大学   Graduate School of Dentistry  

    1986年4月 - 1990年3月

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    国名: 日本国

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  • 岡山大学   Dental School   School of Dentistry

    1980年4月 - 1986年3月

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    国名: 日本国

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経歴

  • 岡山大学   学術研究院 医歯薬学域   教授   歯学博士

    2021年4月 - 現在

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    国名:日本国

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  • 岡山大学   大学院医歯薬学総合研究科 歯周病態学分野   教授   歯学博士

    2005年4月 - 2021年3月

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    国名:日本国

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  • 岡山大学   大学院医歯学総合研究科 歯周病態学分野   教授   歯学博士

    2002年4月 - 2005年3月

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    国名:日本国

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  • 岡山大学   大学院医歯学総合研究科 歯周病態学分野   助教授   歯学博士

    2001年4月 - 2002年3月

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    国名:日本国

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  • 文部科学省在外研究員(USA & NIDCR, USA)

    1996年2月 - 1996年4月

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    国名:アメリカ合衆国

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  • 岡山大学   歯学部   助教授   歯学博士

    1995年11月 - 2001年3月

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    国名:日本国

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  • 岡山大学   歯学部   助手   歯学博士

    1994年12月 - 1995年11月

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    国名:日本国

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  • Eastman Dental Center (Rochester, NY, USA)   Periodontology (Prof. Van Dyke Lab)   客員研究員   歯学博士

    1992年4月 - 1994年11月

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    国名:アメリカ合衆国

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  • 岡山大学   歯学部附属病院   助手   歯学博士

    1990年4月 - 1992年3月

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    国名:日本国

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所属学協会

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委員歴

  • International Association for Dental Research   President of Periodontal Research Group  

    2021年7月 - 現在   

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  • 日本未病学会   理事  

    2020年 - 現在   

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    団体区分:学協会

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  • 日本歯科保存学会   理事  

    2002年4月 - 現在   

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    団体区分:学協会

    日本歯科保存学会

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  • 日本歯周病学会   理事  

    2002年4月 - 現在   

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    団体区分:学協会

    日本歯周病学会

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  • 岡山歯学会   理事  

    2002年4月 - 現在   

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    団体区分:学協会

    岡山歯学会

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  • 日本口腔検査学会   理事,学術委員会委員長  

    2021年4月 - 現在   

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    日本口腔検査学会

  • 日本歯周病学会   常任理事,研究委員会委員長  

    2021年4月 - 2023年3月   

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    団体区分:学協会

  • International Association for Dental Research   President Elect of Periodontal Research Group  

    2020年3月 - 2021年7月   

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  • International Association for Dental Research   President Elect of Periodontal Research  

    2020年3月 - 2021年7月   

  • 日本未病学会   理事  

    2020年 - 現在   

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    団体区分:学協会

    日本未病システム学会から日本未病学会へ改称(2020〜)

  • 日本歯周病学会   常任理事,学会あり方委員会委員長  

    2019年4月 - 2021年3月   

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    団体区分:学協会

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  • 日本歯周病学会   常任理事,学会あり方委員会委員長  

    2019年4月 - 2021年3月   

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    団体区分:学協会

  • International Association for Dental Research   Vice President of Periodontal Research Group  

    2019年3月 - 2020年3月   

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  • International Association for Dental Research   Vice President of Periodontal Research  

    2019年3月 - 2020年3月   

  • International Association for Dental Research   Editorial Board Member  

    2019年1月 - 2021年12月   

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    国際歯科研究学会

  • International Association for Dental Research   Editorial Board Member  

    2019年1月 - 2021年12月   

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  • 日本未病システム学会   理事  

    2019年   

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    団体区分:学協会

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  • 日本未病システム学会   理事  

    2019年   

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    団体区分:学協会

    日本未病システム学会

  • 日本予防医学会   理事  

    2017年9月 - 現在   

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    団体区分:学協会

    日本予防医学会

  • 日本歯科保存学会   常任理事,医療合理化委員会委員長  

    2017年4月 - 2021年3月   

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    団体区分:学協会

    日本歯科保存学会

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  • 日本歯周病学会   常任理事,専門医委員会医員長  

    2017年4月 - 2019年3月   

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    団体区分:学協会

    日本歯周病学会

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  • International Academy of Periodontology   Board Menber  

    2017年1月 - 現在   

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    国際歯周病アカデミー

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  • 日本予防医学会   理事  

    2017年 - 現在   

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    団体区分:学協会

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  • 日本歯周病学会   常任理事,研究委員会医員長  

    2011年4月 - 2015年3月   

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    団体区分:学協会

    日本歯周病学会

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  • 日本口腔検査学会   理事  

    2011年 - 現在   

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    団体区分:学協会

    日本口腔検査学会

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  • 日本歯周病学会   常任理事,用語委員会委員長  

    2009年4月 - 2011年3月   

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    団体区分:学協会

    日本歯周病学会

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  • 日本歯周病学会   2009年(第52回)春季日本歯周病学会 学術大会大会長  

    2009年   

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    団体区分:学協会

    日本歯周病学会

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  • 日本歯科保存学会   常任理事,編集委員会委員長  

    2007年4月 - 2010年3月   

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    団体区分:学協会

    日本歯科保存学会

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  • 日本未病システム学会   評議員  

    2006年 - 2018年   

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    団体区分:学協会

    日本未病システム学会

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  • International Association for Dental Research   Editorial Board Member  

    2001年1月 - 2004年12月   

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    国際歯科研究学会

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  • International Association for Dental Research   Abstract Reviewer (Periodontal Research Group)  

    2000年1月 - 2004年12月   

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    国際歯科研究学会

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論文

  • Functionalized Graphene Oxide Shields Tooth Dentin from Decalcification. 査読 国際誌

    MZI Nizami, Y Nishina, T Yamamoto, Y Shinoda-Ito, S Takashiba

    Journal of dental research   99 ( 2 )   182 - 188   2020年2月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    This in vitro study assessed the efficacy of functionalized graphene oxide (f-GO) nanocomposites on the decalcification of dentin, because dental caries of the root surface is becoming one of the new problems in aged society. Hydroxyapatite plates (HAP) and dentin slices were coated with f-GO nanocomposites by comparing them to silver diamine fluoride as a positive control, then treated with decalcification solutions such as ethylenediaminetetraacetic acid and citrate at 37°C for 24 h. Scanning electron microscopy (SEM) revealed significant protection of the surface morphology of HAP and dentin. On the other hand, a cariogenic Streptococcus mutans growth was inhibited by f-GO nanocomposites. In addition, cytotoxicity of them to epithelial cells was much less than that of povidone-iodine, which is commonly used for oral disinfectant. We synthesized 5 different f-GO nanocomposites such as GO-silver (Ag), GO-Ag-calcium fluoride (CaF2), GO-CaF2, GO-zinc, and GO-tricalcium phosphate (Ca3(PO4)2). They were standardized by evaluating under SEM, transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), thermogravimetry analysis (TGA), and Raman spectra after being synthesized in an aseptic technique. The abilities of GO-Ag, GO-Ag-CaF2, and GO-CaF2 nanocomposites were most preventive for decalcification. In addition, GO-Ag and GO-Ag-CaF2 almost completely inhibited S. mutans growth. However, they did not exhibit cytotoxicity to epithelial cells except at the highest concentration (0.1 w/v%) of GO-Ag and GO-Ag-CaF2. Furthermore, these f-GO nanocomposites exhibited less or no discoloration of dentin, although commonly used silver diamine fluoride causes discoloration of dentin to black. Thus, these f-GO nanocomposites are useful to protect dental caries on the tooth root that becomes a social problem in aged society.

    DOI: 10.1177/0022034519894583

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  • 多施設後ろ向き観察研究による臨床指標としての歯周炎症表面積の基準値 査読

    井上 裕貴, 畑中 加珠, 山本 直史, 平田 貴久, 三辺 正人, 山本 龍生, 内藤 徹, 山本 松男, 佐藤 秀一, 石幡 浩志, 稲垣 幸司, 三谷 章雄, 中島 啓介, 漆原 譲治, 高柴 正悟

    日本歯周病学会会誌   61 ( 4 )   159 - 167   2019年12月

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    担当区分:最終著者, 責任著者   記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:(NPO)日本歯周病学会  

    歯周炎症表面積(periodontal inflamed surface area:PISA)は,歯周組織の炎症部の面積を表す新たな歯周病の臨床指標である。従来伝わりづらかった歯周病の炎症程度を歯科以外の医療従事者が理解する上で,有用な指標であると考えられる。しかし,歯周病の程度や治療によるPISAの基準は未だ不明である。そこで,本研究は,日本歯周病学会が設ける歯周病専門医・認定医の電子申請書類のデータから各治療フェーズにおけるPISAの値を調べ,歯周病治療に伴う炎症度の基準値を提案することを目的とした。8施設で取得した113症例を用いて,Nesseらの方法によって歯周ポケット深さとプロービング時の出血からPISAを算出した。その結果,PISAの中央値は,初診時1,271.4mm2,歯周基本治療終了時211.8mm2,SPT移行時52.1mm2,そして最新SPT時30.0mm2であった。また,PISAはBOPと高い相関を示し(p<0.001),BOPよりも鋭敏に治療効果を反映した。以上から,中等度以上の歯周炎においてPISAを用いると,初診時は表面積約1,500mm2の歯周組織の炎症がSPT時は100mm2未満(初診時の約7%)に減少することが明らかになった。今後,更なるデータの蓄積および詳細な分析を行いながらPISAの使用を普及させると,PISAは医科歯科連携の際に歯周病炎症を伝える指標になり得ると考える。(著者抄録)

    DOI: 10.2329/perio.61.159

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  • Acceleration of bone regeneration of horizontal bone defect in rats using collagen-binding basic fibroblast growth factor combined with collagen scaffolds. 査読 国際誌

    Shin Nakamura, Takashi Ito, Kentaro Okamoto, Takehiko Mima, Kentaro Uchida, Yasir D Siddiqui, Masahiro Ito, Masako Tai, Keisuke Okubo, Keisuke Yamashiro, Kazuhiro Omori, Tadashi Yamamoto, Osamu Matsushita, Shogo Takashiba

    Journal of periodontology   90 ( 9 )   1043 - 1052   2019年9月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Basic fibroblast growth factor (bFGF) has been applied for periodontal regeneration. However, the application depends on bone defect morphology because bFGF diffuses rapidly from defect sites. In a previous study, collagen-binding bFGF (CB-bFGF) has been shown to enhance bone formation by collagen-anchoring in the orthopedic field. The aim of this study is to demonstrate the efficacy of CB-bFGF with collagen scaffolds in bone regeneration of horizontal bone defect. METHODS: Cell proliferation activity and collagen binding activity of CB-bFGF was confirmed by WST-8 assay and collagen binding assay, respectively. The retention of CB-bFGF in the collagen sheet (CS) was measured by fluorescence imaging. The rat horizontal alveolar bone defect model was employed to investigate the efficacy of CB-bFGF with collagen powder (CP). After 4 and 8 weeks, the regenerative efficacy was evaluated by microcomputed tomography, histological, and immunohistochemical analyses. RESULTS: CB-bFGF had a comparable proliferation activity to bFGF and a collagen binding activity. CB-bFGF was retained in CS longer than bFGF. At 8 weeks postoperation, bone volume, bone mineral content, and new bone area in CB-bFGF/CP group were significantly increased compared with those in other groups. Furthermore, epithelial downgrowth was significantly suppressed in CB-bFGF/CP group. At 4 weeks, the numbers of osteocalcin, proliferating cell nuclear antigen, and osteopontin-positive cells at the regeneration site in CB-bFGF/CP group were greater than those in other groups. CONCLUSIONS: CB-bFGF/CP effectively promoted bone regeneration of horizontal bone defect possibly by sustained release of bFGF. The potential of CB-bFGF composite material for improved periodontal regeneration in vertical axis was shown.

    DOI: 10.1002/JPER.18-0674

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  • Resolvin D2 Induces Resolution of Periapical Inflammation and Promotes Healing of Periapical Lesions in Rat Periapical Periodontitis 査読 国際共著 国際誌

    Yasir Dilshad Siddiqui, Kazuhiro Omori, Takashi Ito, Keisuke Yamashiro, Shin Nakamura, Kentaro Okamoto, Mitsuaki Ono, Tadashi Yamamoto, Thomas E. Van Dyke, Shogo Takashiba

    Frontiers in Immunology   10   2019年2月

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    担当区分:最終著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Frontiers Media SA  

    DOI: 10.3389/fimmu.2019.00307

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  • Quantitative real-time PCR using TaqMan and SYBR Green forActinobacillus actinomycetemcomitans,Porphyromonas gingivalis,Prevotella intermedia,tetQgene and total bacteria 査読

    Hiroshi Maeda, Chiyo Fujimoto, Yasuhiro Haruki, Takemasa Maeda, Susumu Kokeguchi, Millan Petelin, Hideo Arai, Ichiro Tanimoto, Fusanori Nishimura, Shogo Takashiba

    FEMS Immunology & Medical Microbiology   39 ( 1 )   81 - 86   2003年10月

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    担当区分:責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

    DOI: 10.1016/s0928-8244(03)00224-4

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  • Induced TNF-alpha and IL1-beta Production by Human Monocytes 査読 国際共著 国際誌

    L Shapira, S Takashiba, C Champagne, S Amar, TE Van Dyke

    JOURNAL OF IMMUNOLOGY   153 ( 4 )   1818 - 1824   1994年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC IMMUNOLOGISTS  

    Bacterial LPS stimulates human monocytes to secrete inflammatory cytokines, which are involved in several disease processes. However, the mechanism of LPS activation of cytokine expression and secretion is not completely understood. In this study, we investigated the signal transduction pathways involved in LPS-stimulated TNF-alpha and IL-1 beta secretion. TNF-alpha and IL-1 beta secretion were completely blocked by protein kinase C (PKC) and cyclic nucleotide-dependent protein kinase inhibitor, H-7, but were not affected by H-89, a specific cyclic nucleotide-dependent protein kinase inhibitor. In addition, LPS was found to induce activation of PKC, reaching maximal activity at 30 min and returning to unstimulated levels after 60 min. LPS stimulation only slightly increased intracellular levels of diacylglycerol, the natural activator of PKC, and pretreatment of monocytes with the diacylglycerol-kinase inhibitor, R59022, did not affect LPS-stimulated TNF-alpha secretion. LPS-induced PKC activation was found not to be affected by blocking of the LPS receptor, CD14, with mAb or by inhibition of protein tyrosine kinase with herbimycin A. However, these agents suppressed LPS-induced TNF-alpha secretion and TNF-alpha mRNA accumulation. The results suggest that TNF-alpha and IL-1 beta secretion after LPS stimulation of human monocytes requires the activation of protein tyrosine kinase and PKC, upstream to the activation of gene transcription. The activation of PKC by LPS is probably mediated by a diacylglycerol-independent pathway.

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  • A cross-sectional study assessing the relationship between non-alcoholic fatty liver disease and periodontal disease

    Satsuki Sato, Yohei Kamata, Takaomi Kessoku, Tomoko Shimizu, Takashi Kobayashi, Takeo Kurihashi, Shogo Takashiba, Kazu Hatanaka, Nobushiro Hamada, Toshiro Kodama, Takuma Higurashi, Masataka Taguri, Masato Yoneda, Haruki Usuda, Koichiro Wada, Atsushi Nakajima, Toshiya Morozumi, Masato Minabe

    Scientific Reports   12 ( 1 )   2022年8月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    Abstract

    The risk factors for non-alcoholic fatty liver disease (NAFLD) progression are not completely known. Porphyromonasgingivalis infection is a risk factor for systemic diseases. We investigated the association of P.gingivalis infection with the risk of non-alcoholic steatohepatitis progression. Here, hematological tests, periodontal examination, and saliva collection were performed for 164 patients with NAFLD. P.gingivalis was identified in saliva using polymerase chain reaction. Hepatic steatosis and stiffness were evaluated using vibration-controlled transient elastography (VCTE) and magnetic resonance imaging. In patients with NAFLD, P.gingivalis positivity (P.gingivalis ratio ≥ 0.01%) in saliva correlated with liver stiffness determined using magnetic resonance elastography (MRE; p &lt; 0.0001). A P.gingivalis ratio of 0.01% corresponds to 100,000 cells/mL and indicates the proportion of P.gingivalis in the total number of bacteria in the oral cavity. Patients with NAFLD and advanced fibrosis on MRE showed significantly elevated endotoxin activity; those who had &gt; 10 periodontal pockets with depths ≥ 4 mm had significantly increased hepatic stiffness on both VCTE and MRE.

    DOI: 10.1038/s41598-022-17917-2

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    その他リンク: https://www.nature.com/articles/s41598-022-17917-2

  • Treatment resistance of rheumatoid arthritis relates to infection of periodontal pathogenic bacteria: a case-control cross-sectional study. 国際誌

    Kazu Takeuchi-Hatanaka, Yoshinobu Koyama, Kentaro Okamoto, Kyosuke Sakaida, Tadashi Yamamoto, Shogo Takashiba

    Scientific reports   12 ( 1 )   12353 - 12353   2022年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Recent studies have shown that periodontitis is associated with rheumatoid arthritis (RA) and periodontal bacteria, such as Aggregatibacter actinomycetemcomitans (Aa) and Porphyromonas gingivalis (Pg) are involved in the pathogenesis of RA via citrullinated proteins. Smoking has also been shown to be involved in the pathogenesis of RA; however, the extent of this involvement is still poorly understood. In addition, RA and polymyalgia rheumatica (PMR) are sometimes difficult to differentiate; however, the relationship between PMR and the factors from smoking and periodontal bacteria is unclear. The aim of this study was to clarify the relationship between periodontal pathogenic bacterial infections and smoking in patients with RA or PMR. This case-control study included 142 patients with untreated RA or PMR. This study evaluated the serum antibody titers against periodontal pathogenic bacterial antigens and an anti-citrullinated peptide antibody (ACPA). In patients with RA, the relationship between antibody titers and disease activity of RA and response after 3 months of treatment was also investigated. Additionally, the effects of smoking were evaluated. Although there was no significant difference in serum antibody titer against periodontal pathogenic bacteria between the ACPA-positive RA group and the ACPA-negative PMR group, we found an association between the elevated antibody titer against Pg and the degree of ACPA value, especially between negative group and high-value positive group (≥ 100 U/mL). The antibody titers against Aa and Pg did not differ depending on disease activity score 28 (DAS28) at baseline; however, patients with high antibody titers had poor RA therapeutic response as judged by DAS28 after 3 months. We could not find any association between smoking and any of these parameters. Periodontal pathogenic bacteria, especially Pg, are associated with elevated ACPA levels. Our findings suggest that Pg and Aa infections interfere with the therapeutic response of RA.

    DOI: 10.1038/s41598-022-16279-z

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  • Analysis of subgingival microbiota in monozygotic twins with different severity and progression risk of periodontitis. 国際誌

    Tadashi Yamamoto, Makoto Taniguchi, Kazuyuki Matsunaga, Yusuke Kawata, Mari Kawamura, Keisuke Okubo, Keisuke Yamashiro, Kazuhiro Omori, Shogo Takashiba

    Clinical case reports   10 ( 4 )   e05725   2022年4月

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    記述言語:英語  

    The study aims to reveal the composition of subgingival bacteria in monozygotic twins with discordant in severity and progression risk of periodontitis. Microbiome analysis indicated that most bacteria were heritable but differed in their abundance and immune response. The dysbiotic bacteria can be considered as risk markers for periodontitis progression.

    DOI: 10.1002/ccr3.5725

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  • Estimation of periodontal pocket surface area in small to medium dogs: a proof-of-concept study. 国際誌

    Kazuya Tamura, Masako Tokuzen-Tai, Yasir Dilshad Siddiqui, Hitomi Tamura-Naito, Yoshiharu Nagahara, Kazu Hatanaka-Takeuchi, Tadashi Yamamoto, Shogo Takashiba

    BMC veterinary research   18 ( 1 )   13 - 13   2022年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Periodontal disease is the most common dental disease in dogs. Although the systemic effects of periodontal disease have not been clarified in veterinary science, it is necessary to evaluate the effects of periodontal disease in clinical trials in the future. There have been a few clinical attempts made, however, to assess the severity of periodontal inflammation and its impact on the systemic health of dogs. Meanwhile, in the field of dentistry for humans, the periodontal inflamed surface area (PISA) and periodontal epithelial surface area (PESA) have been used to quantitatively assess the degree of periodontal disease affecting a single tooth as well as the overall extent of periodontitis. Recent studies have also suggested the use of these assessments to examine the relationship between periodontal inflammation and systemic health. RESULTS: The estimation formula for a dog's periodontal pocket surface area (PPSA), an alternative to PISA and PESA in humans, was established using body weight and periodontal pocket depth. Actual values were measured using extracted teeth from various dog breeds and sizes (2.3-25.0 kg of body weight) to obtain universal regression equations for PPSA. Altogether, 625 teeth from 73 dogs of 16 breeds were extracted and subsequently analyzed for morphological information. PPSA was measured in 61 dogs of 10 breeds with periodontal disease using the established estimation formulas, and the correlation between PPSA and preoperative blood chemistry data was analyzed accordingly. A strong correlation was found between PPSA and serum globulin (r = 0.71) while moderate correlations were found for C-reactive protein (r = 0.54) and serum albumin (r = -0.51). CONCLUSIONS: Estimation formulas using body weight and the 6-point probing depth were established for determining PPSA. Direct correlations between PPSA and several blood test results were observed in the study sample. Taken together, these results suggest that PPSA could be useful for evaluating the effects of periodontitis on systemic conditions in dogs.

    DOI: 10.1186/s12917-021-03116-0

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  • Enzymatic measurement of short-chain fatty acids and application in periodontal disease diagnosis. 国際誌

    Kazu Hatanaka, Yasushi Shirahase, Toshiyuki Yoshida, Mari Kono, Naoki Toya, Shin-Ichi Sakasegawa, Kenji Konishi, Tadashi Yamamoto, Kuniyasu Ochiai, Shogo Takashiba

    PloS one   17 ( 7 )   e0268671   2022年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Periodontal disease is a chronic inflammatory condition caused by periodontal pathogens in the gingival sulcus. Short-chain fatty acids (SCFAs) produced by causal bacteria are closely related to the onset and progression of periodontal disease and have been reported to proliferate in the periodontal sulcus of patients experiencing this pathology. In such patients, propionic acid (C3), butyric acid (C4), isobutyric acid (IC4), valeric acid (C5), isovaleric acid (IC5), and caproic acid (C6), henceforth referred to as [C3-C6], has been reported to have a detrimental effect, while acetic acid (C2) exhibits no detrimental effect. In this study, we established an inexpensive and simple enzymatic assay that can fractionate and measure these acids. The possibility of applying this technique to determine the severity of periodontal disease by adapting it to specimens collected from humans has been explored. We established an enzyme system using acetate kinase and butyrate kinase capable of measuring SCFAs in two fractions, C2 and [C3-C6]. The gingival crevicular fluid (GCF) and saliva of 10 healthy participants and 10 participants with mild and severe periodontal disease were measured using the established enzymatic method and conventional gas chromatography-mass spectrometry (GC-MS). The quantification of C2 and [C3-C6] in human GCF and saliva was well correlated when using the GC-MS method. Furthermore, both C2 and [C3-C6] in the GCF increased with disease severity. However, while no significant difference was observed between healthy participants and periodontal patients when using saliva, [C3-C6] significantly differed between mild and severe periodontal disease. The enzymatic method was able to measure C2 and [C3-C6] separately as well as using the GC-MS method. Furthermore, the C2 and [C3-C6] fractions of GCF correlated with disease severity, suggesting that this method can be applied clinically. In contrast, the quantification of C2 and [C3-C6] in saliva did not differ significantly between healthy participants and patients with periodontal disease. Future studies should focus on inflammation rather than on tissue destruction.

    DOI: 10.1371/journal.pone.0268671

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  • 歯周病ポケット検査における代表歯・部位の選定

    両角 俊哉, 野村 義明, 中川 種昭, 沼部 幸博, 菅谷 勉, 佐藤 聡, 野口 和行, 吉成 伸夫, 吉村 篤利, 西村 英紀, 齋藤 淳, 福田 光男, 小林 宏明, 菅野 直之, 多部田 康一, 高橋 慶壮, 梅田 誠, 高柴 正悟, 三邉 正人, 小方 頼昌

    神奈川歯学   56 ( Abstracts )   P - 39   2021年12月

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    記述言語:日本語   出版者・発行元:神奈川歯科大学学会  

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  • Use of Highly Accurate Devices for a First Lower Premolar Endodontic Treatment with Multiple Root Canals. 査読 国際共著 国際誌

    Zulema Arias Martinez, Jorge Lopez Videla, Keisuke Yamashiro, Yuki Shinoda-Ito, Tadashi Yamamoto, Shogo Takashiba

    Acta medica Okayama   75 ( 5 )   641 - 645   2021年10月

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    担当区分:最終著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    This case report highlights the importance of using a dental operating microscope (DOM) and ultrasonic endodontic tips (UETs) to locate all root canals in the lower first premolar. A 53-year-old woman presented to our clinic with pain in the lower right first premolar. After a detailed search using a DOM and UETs, three root canals were found, prepared with rotary HyFlex endodontic files, and obturated using the lateral condensation technique. At the five-year follow-up after treatment, the tooth was completely restored and fulfilling its function, with no signs or symptoms of any post-treatment flare-up.

    DOI: 10.18926/AMO/62778

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  • 歯周病のポケット検査における代表歯・部位の選定

    両角 俊哉, 高柴 正悟, 三邉 正人, 野村 義明, 福田 光男, 花田 信弘, 角田 衣理加, 小林 宏明, 中村 利明, 中山 洋平, 西村 英紀, 野口 和行, 沼部 幸博, 小方 頼昌, 齋藤 淳, 佐藤 聡, 関野 愉, 菅野 直之, 菅谷 勉, 鈴木 史彦, 多部田 康一, 高橋 慶壮, 高井 英樹, 梅田 誠, 吉村 篤利, 吉成 伸夫, 中川 種昭

    日本口腔検査学会総会・学術大会プログラム・抄録集   14回   70 - 71   2021年8月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔検査学会  

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  • Malnutrition delayed wound healing after tooth extraction by HMGB1-related prolonged inflammation. 査読 国際誌

    Yao Zhang, Hidetaka Ideguchi, Hiroaki Aoyagi, Keisuke Yamashiro, Tadashi Yamamoto, Masahiro Nishibori, Shogo Takashiba

    International immunopharmacology   96   107772 - 107772   2021年7月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Malnutrition causes prolonged inflammation, resulting in delayed wound healing. High mobility group box-1 (HMGB1) is a damage-associated molecular pattern that is present in the nuclei of macrophages and is secreted into the extracellular milieu in response to stimuli. It stimulates the production of interleukin-1β (IL-1β) through the receptors for advanced glycation end products (RAGE), inducing an inflammatory response, which is an essential response to initiate wound healing. We hypothesized that malnutrition may interfere with this cascade, causing abnormal inflammation and ultimately delaying wound healing. We used tooth-extracted mice with malnutrition fed with low-casein diet for two weeks. On days 3 and 7 after tooth extraction, the wound tissue was histologically observed and analyzed for several factors in the inflammation-regeneration lineage, including IL-1β, mesenchymal stem cells, myeloperoxidase activity, HMGB1, macrophage polarization, and adenosine 5-triphosphate (ATP). On day 7, delayed wound healing was observed with the following findings under malnutrition conditions: decreased mRNA expression of genes for regeneration and mesenchymal stem cell (MSC) accumulation, an obvious increase in myeloperoxidase and IL-1β mRNA expression, an increase in HMGB1 levels, and an increase in ATP concentration in tissues with elevated proportion of M2 macrophages. These results suggest that the significantly increased secretion of HMGB1 associated with the upregulated production of ATP and IL-1β secretion via the RAGE pathway may interfere with the resolution of inflammation and wound healing under the state of malnutrition.

    DOI: 10.1016/j.intimp.2021.107772

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  • The Fungal Metabolite (+)-Terrein Abrogates Ovariectomy-Induced Bone Loss and Receptor Activator of Nuclear Factor-κB Ligand-Induced Osteoclastogenesis by Suppressing Protein Kinase-C α/βII Phosphorylation. 査読 国際誌

    Kyosuke Sakaida, Kazuhiro Omori, Masaaki Nakayama, Hiroki Mandai, Saki Nakagawa, Hidefumi Sako, Chiaki Kamei, Satoshi Yamamoto, Hiroya Kobayashi, Satoki Ishii, Mitsuaki Ono, Soichiro Ibaragi, Keisuke Yamashiro, Tadashi Yamamoto, Seiji Suga, Shogo Takashiba

    Frontiers in pharmacology   12   674366 - 674366   2021年6月

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    担当区分:最終著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Osteoporosis is a common disease characterized by a systemic impairment of bone mass and microarchitecture that results in fragility fractures. Severe bone loss due to osteoporosis triggers pathological fractures and consequently decreases the daily life activity and quality of life. Therefore, prevention of osteoporosis has become an important issue to be addressed. We have reported that the fungal secondary metabolite (+)-terrein (TER), a natural compound derived from Aspergillus terreus, has shown receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation by suppressing nuclear factor of activated T-cell 1 (NFATc1) expression, a master regulator of osteoclastogenesis. TER has been shown to possess extensive biological and pharmacological benefits; however, its effects on bone metabolism remain unclear. In this study, we investigated the effects of TER on the femoral bone metabolism using a mouse-ovariectomized osteoporosis model (OVX mice) and then on RANKL signal transduction using mouse bone marrow macrophages (mBMMs). In vivo administration of TER significantly improved bone density, bone mass, and trabecular number in OVX mice (p < 0.01). In addition, TER suppressed TRAP and cathepsin-K expression in the tissue sections of OVX mice (p < 0.01). In an in vitro study, TER suppressed RANKL-induced phosphorylation of PKCα/βII, which is involved in the expression of NFATc1 (p < 0.05). The PKC inhibitor, GF109203X, also inhibited RANKL-induced osteoclastogenesis in mBMMs as well as TER. In addition, TER suppressed the expression of osteoclastogenesis-related genes, such as Ocstamp, Dcstamp, Calcr, Atp6v0d2, Oscar, and Itgb3 (p < 0.01). These results provide promising evidence for the potential therapeutic application of TER as a novel treatment compound against osteoporosis.

    DOI: 10.3389/fphar.2021.674366

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  • Association between Psychosocial Factors and Oral Symptoms among Residents in Fukushima after the Great East Japan Earthquake: A Cross-Sectional Study from the Fukushima Health Management Survey. 査読 国際誌

    Narumi Funakubo, Ayaka Tsuboi, Eri Eguchi, Fumikazu Hayashi, Masaharu Maeda, Hirooki Yabe, Seiji Yasumura, Kenji Kamiya, Shogo Takashiba, Tetsuya Ohira, Mental Health Group Of The Fukushima Health Management Survey

    International journal of environmental research and public health   18 ( 11 )   2021年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Oral health is closely related to subjective general health and systemic diseases. This cross-sectional study aimed to identify the factors related to oral symptoms and their worsening in relation to psychosocial factors after the Great East Japan Earthquake. In this study, 64,186 residents aged 15-101 years old, who experienced the earthquake on 11 March 2011, were surveyed regarding their oral symptoms; psychological factors, such as post-traumatic reactions and psychological distress; and social factors such as evacuation, work change, and loss of a close person; history of systemic diseases; and lifestyle. Binomial logistic regression analysis was used to calculate odds ratios, and 95% confidence intervals were established for each factor associated with prevalent and exacerbated oral symptoms. The proportions of participants with prevalent and exacerbated oral symptoms were 10.3% and 1.6%, respectively. The multivariate odds ratios and 95% CI of psychosocial factors associated with exacerbated oral symptoms were as follows: post-traumatic stress disorder symptoms, 2.24 (1.64-3.06); work changes, 1.88 (1.34-2.65); history of dyslipidemia, 1.74 (1.27-2.39); and subjective current poor health condition, 2.73 (2.00-3.75). Psychological factors, social factors, and physical factors were associated with both prevalent and exacerbated oral symptoms.

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  • Prospective Longitudinal Changes in the Periodontal Inflamed Surface Area Following Active Periodontal Treatment for Chronic Periodontitis. 査読 国際誌

    Yoshiaki Nomura, Toshiya Morozumi, Atsushi Saito, Atsutoshi Yoshimura, Erika Kakuta, Fumihiko Suzuki, Fusanori Nishimura, Hideki Takai, Hiroaki Kobayashi, Kazuyuki Noguchi, Keiso Takahashi, Koichi Tabeta, Makoto Umeda, Masato Minabe, Mitsuo Fukuda, Naoyuki Sugano, Nobuhiro Hanada, Nobuo Yoshinari, Satoshi Sekino, Shogo Takashiba, Soh Sato, Toshiaki Nakamura, Tsutomu Sugaya, Yohei Nakayama, Yorimasa Ogata, Yukihiro Numabe, Taneaki Nakagawa

    Journal of clinical medicine   10 ( 6 )   2021年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Periodontal disease is a chronic inflammatory disease of the periodontal tissue. The periodontal inflamed surface area (PISA) is a proposed index for quantifying the inflammatory burden resulting from periodontitis lesions. This study aimed to investigate longitudinal changes in the periodontal status as evaluated by the PISA following the active periodontal treatment. To elucidate the prognostic factors of PISA, mixed-effect modeling was performed for clinical parameters, tooth-type, and levels of periodontal pathogens as independent variables. One-hundred-twenty-five patients with chronic periodontitis who completed the active periodontal treatment were followed-up for 24 months, with evaluations conducted at 6-month intervals. Five-times repeated measures of mean PISA values were 130+/-173, 161+/-276, 184+/-320, 175+/-417, and 209+/-469 mm2. Changes in clinical parameters and salivary and subgingival periodontal pathogens were analyzed by mixed-effect modeling. Plaque index, clinical attachment level, and salivary levels of Porphyromonas gingivalis were associated with changes in PISA at the patient- and tooth-level. Subgingival levels of P. gingivalis and Prevotella intermedia were associated with changes in PISA at the sample site. For most patients, changes in PISA were within 10% of baseline during the 24-month follow-up. However, an increase in the number of bleeding sites in a tooth with a deep periodontal pocket increased the PISA value exponentially.

    DOI: 10.3390/jcm10061165

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  • Estimation of the Periodontal Inflamed Surface Area by Simple Oral Examination. 査読 国際誌

    Yoshiaki Nomura, Toshiya Morozumi, Yukihiro Numabe, Yorimasa Ogata, Yohei Nakayama, Tsutomu Sugaya, Toshiaki Nakamura, Soh Sato, Shogo Takashiba, Satoshi Sekino, Nobuo Yoshinari, Nobuhiro Hanada, Naoyuki Sugano, Mitsuo Fukuda, Masato Minabe, Makoto Umeda, Koichi Tabeta, Keiso Takahashi, Kazuyuki Noguchi, Hiroaki Kobayashi, Hideki Takai, Fusanori Nishimura, Fumihiko Suzuki, Erika Kakuta, Atsutoshi Yoshimura, Atsushi Saito, Taneaki Nakagawa

    Journal of clinical medicine   10 ( 4 )   2021年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The periodontal inflamed surface area (PISA) is a useful index for clinical and epidemiological assessments, since it can represent the inflammation status of patients in one contentious variable. However, calculation of the PISA is difficult, requiring six point probing depth measurements with or without bleeding on probing on 28 teeth, followed by data input in a calculation program. More simple methods are essential for screening periodontal disease or in epidemiological studies. In this study, we tried to establish a convenient partial examination method to estimate PISA. Cross-sectional data of 254 subjects who completed active periodontal therapy were analyzed. Teeth that represent the PISA value were selected by an item response theory approach. The maxillary second molar, first premolar, and lateral incisor and the mandibular second molar and lateral incisor were selected. The sum of the PISAs of these teeth was significantly correlated with the patient's PISA (R2 = 0.938). More simply, the sum of the maximum values of probing pocket depth with bleeding for these teeth were also significantly correlated with the patient's PISA (R2 = 0.6457). The simple model presented in this study may be useful to estimate PISA.

    DOI: 10.3390/jcm10040723

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  • Microbiome composition comparison in oral and atherosclerotic plaque from patients with and without periodontitis. 査読 国際誌

    Daichi Isoshima, Keisuke Yamashiro, Kazuyuki Matsunaga, Makoto Taniguchi, Takehiro Matsubara, Shuta Tomida, Shinzo Ota, Michiyoshi Sato, Yutaka Shimoe, Tatsuo Kohriyama, Zulema Arias, Kazuhiro Omori, Tadashi Yamamoto, Shogo Takashiba

    Odontology   109 ( 1 )   239 - 249   2021年1月

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    担当区分:最終著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    There is no conclusive evidence regarding a causal relationship between periodontitis and atherosclerosis. In this study, we examined the microbiome in the oral cavity and atheromatous plaques from atherosclerosis patients with or without periodontitis to investigate the role of oral bacteria in the formation of atheromatous plaques. We chose four patients with and without periodontitis, who had undergone carotid endarterectomy. Bacterial samples were extracted from the tongue surface, from periodontal pocket (during the oral examination), and from the atheromatous plaques (APs). We investigated the general and oral conditions from each patient and performed next-generation sequencing (NGS) analysis for all bacterial samples. There were no significant differences between both groups concerning general conditions. However, the microbiome patterns of the gingival pocket showed differences depending on the absence or presence of periodontitis, while those of the tongue surface were relatively similar. The microbiome pattern of the atheromatous plaques was entirely different from that on the tongue surface and gingival pocket, and oral bacteria were seldom detected. However, the microbiome pattern in atheromatous plaques was different in the presence or absence of periodontitis. These results suggested that oral bacteria did not affect the formation of atheromatous plaques directly.

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  • Allergic Reaction to Zirconia Ceramic Bridge Cementation Using a Dental Adhesive Resin Cement: a Case Report 査読 国際誌

    Keisuke Yamashiro, Haruna Miki, Masae Kitagawa, Hiroko Oka, Zulema Arias, Shogo Takashiba

    SN Comprehensive Clinical Medicine   3 ( 1 )   327 - 330   2021年1月

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    担当区分:最終著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    DOI: 10.1007/s42399-020-00671-9

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  • Optimal Examination Sites for Periodontal Disease Evaluation: Applying the Item Response Theory Graded Response Model. 査読 国際誌

    Yoshiaki Nomura, Toshiya Morozumi, Mitsuo Fukuda, Nobuhiro Hanada, Erika Kakuta, Hiroaki Kobayashi, Masato Minabe, Toshiaki Nakamura, Yohei Nakayama, Fusanori Nishimura, Kazuyuki Noguchi, Yukihiro Numabe, Yorimasa Ogata, Atsushi Saito, Soh Sato, Satoshi Sekino, Naoyuki Sugano, Tsutomu Sugaya, Fumihiko Suzuki, Keiso Takahashi, Hideki Takai, Shogo Takashiba, Makoto Umeda, Hiromasa Yoshie, Atsutoshi Yoshimura, Nobuo Yoshinari, Taneaki Nakagawa

    Journal of clinical medicine   9 ( 11 )   2020年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Periodontal examination data have a complex structure. For epidemiological studies, mass screenings, and public health use, a simple index that represents the periodontal condition is necessary. Periodontal indices for partial examination of selected teeth have been developed. However, the selected teeth vary between indices, and a justification for the selection of examination teeth has not been presented. We applied a graded response model based on the item response theory to select optimal examination teeth and sites that represent periodontal conditions. Data were obtained from 254 patients who participated in a multicenter follow-up study. Baseline data were obtained from initial follow-up. Optimal examination sites were selected using item information calculated by graded response modeling. Twelve sites-maxillary 2nd premolar (palatal-medial), 1st premolar (palatal-distal), canine (palatal-medial), lateral incisor (palatal-central), central incisor (palatal-distal) and mandibular 1st premolar (lingual, medial)-were selected. Mean values for clinical attachment level, probing pocket depth, and bleeding on probing by full mouth examinations were used for objective variables. Measuring the clinical parameters of these sites can predict the results of full mouth examination. For calculating the periodontal index by partial oral examination, a justification for the selection of examination sites is essential. This study presents an evidence-based partial examination methodology and its modeling.

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  • Follistatin expressed in mechanically-damaged salivary glands of male mice induces proliferation of CD49f+ cells. 査読 国際誌

    A Ikeda, T Yamamoto, J Mineshiba, S Takashiba

    Scientific reports   10 ( 1 )   19959 - 19959   2020年11月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Salivary glands (SGs) are very important for maintaining the physiological functions of the mouth. When SGs regenerate and repair from various damages, including mechanical, radiological, and immune diseases, acinar and granular duct cells originate from intercalated duct cells. However, the recovery is often insufficient because of SGs' limited self-repair function. Furthermore, the precise repair mechanism has been unclear. Here, we focused on CD49f, one of the putative stem cell markers, and characterized CD49f positive cells (CD49f+ cells) isolated from male murine SGs. CD49f+ cells possess self-renewal ability and express epithelial and pluripotent markers. Compared to CD49f negative cells, freshly isolated CD49f+ cells highly expressed inhibin beta A and beta B, which are components of activin that has anti-proliferative effects. Notably, an inhibitor of activin, follistatin was expressed in mechanically-damaged SGs, meanwhile no follistatin was expressed in normal SGs in vivo. Moreover, sub-cultured CD49f+ cells highly expressed both Follistatin and a series of proliferative genes, expressions of which were decreased by Follistatin siRNA. These findings indicated that the molecular interaction between activin and follistatin may induce CD49f+ cells proliferation in the regeneration and repair of mouse SGs.

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  • Isolation and identification of the antimicrobial substance included in tempeh using Rhizopus stolonifer NBRC 30816 for fermentation. 査読 国際誌

    Masahiro Ito, Takashi Ito, Hideyuki Aoki, Koshi Nishioka, Tsugumi Shiokawa, Hiroko Tada, Yuki Takeuchi, Nobuyuki Takeyasu, Tadashi Yamamoto, Shogo Takashiba

    International journal of food microbiology   325   108645 - 108645   2020年7月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In this study, we focus on the antimicrobial properties of tempeh, a soybean fermented food, against oral bacteria. Tempeh showed antimicrobial activity against dental caries pathogenic bacterium Streptococcus mutans at a final concentration of 1 mg/mL. An antimicrobial substance contained in tempeh was present in the 100 kDa or greater fraction generated by ultrafiltration, but it was found not to be proteinaceous by native-PAGE, SDS-PAGE and protein degradation tests. Next, when the fraction was purified with an ODS column, the 80% and 100% methanol eluates showed antimicrobial activity against S. mutans. The 100% methanol eluate was further subjected to a 2nd column purification, and isolation of the target was confirmed by HPLC. When the isolated material was analyzed by ESI-MS, the m/z was 279.234. Further analysis by Raman spectroscopy revealed a peak similar to linoleic acid. This substance also possessed antimicrobial properties equivalent to linoleic acid.

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  • Identification and Modification of Porphyromonas gingivalis Cysteine Protease, Gingipain, Ideal for Screening Periodontitis. 査読 国際誌

    Kimito Hirai, Tomoko Yamaguchi-Tomikawa, Toru Eguchi, Hiroshi Maeda, Shogo Takashiba

    Frontiers in immunology   11   1017 - 1017   2020年6月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Chronic periodontitis is an inflammatory disease caused by the formation of oral microbial biofilms. Periodontitis is associated with general health and not only oral diseases. Porphyromonas gingivalis is a well-known keystone pathogen for periodontitis and is associated with several systemic diseases, such as diabetes mellitus and Alzheimer's disease. We previously developed a system for screening periodontitis using P. gingivalis-specific serum immunoglobulin G (IgG) in an enzyme-linked immunosorbent assay with a sensitivity of 0.774 and a specificity of 0.586 and an area under the receiver operating characteristic curve of 0.708. However, the antigens elicited non-specific responses, since they were obtained from whole extracts of sonicated cultured bacteria. The purpose of this study was to identify antigens ideal for a sensitive and specific serum test. We identified the specific antigens using immunoaffinity columns immobilized with IgG antibodies from periodontitis patients. Liquid chromatography-tandem mass spectrometry identified 29 antigens from the elutes. Recombinant proteins for these candidates were synthesized using the wheat germ cell-free translation system and screened by dot blot analysis with serum from the columns. Three of the 16 candidates that reacted showed strongest affinities upon dot blot analysis; they included outer membrane protein 28, cysteine proteases, lysine gingipain Kgp, and arginine gingipain RgpA. Outer membrane protein 28 was not suitable for screening P. gingivalis infection because of its high false-negative rates. Kgp and RgpA were unstable antigens since they underwent self-digestion. They were made stable by substituting the active cysteine residues in Kgp and RgpA with alanine using site-directed mutagenesis. Using the modified antigens, we demonstrated that the patient serum IgG level against RgpA was the highest among all the antigens expressed in P. gingivalis. Moreover, the N-terminus of recombinant RgpA was excellent in differentiating between diseased and non-diseased states (with sensitivity of 0.85, specificity of 0.9, and area under the curve of 0.915). Although dot blot analysis was the only experiment used, the N-terminus of RgpA is an excellent antigen to immunologically test for P. gingivalis infection, especially for estimating the risks for periodontitis-associated systemic diseases. In conclusion, we have developed a P. gingivalis antigen for screening periodontitis.

    DOI: 10.3389/fimmu.2020.01017

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  • The fungal metabolite (+)-terrein abrogates osteoclast differentiation via suppression of the RANKL signaling pathway through NFATc1. 査読 国際誌

    Saki Nakagawa, Kazuhiro Omori, Masaaki Nakayama, Hiroki Mandai, Satoshi Yamamoto, Hiroya Kobayashi, Hidefumi Sako, Kyosuke Sakaida, Hiroshi Yoshimura, Satoki Ishii, Soichiro Ibaragi, Kimito Hirai, Keisuke Yamashiro, Tadashi Yamamoto, Seiji Suga, Shogo Takashiba

    International immunopharmacology   83   106429 - 106429   2020年6月

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    担当区分:最終著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Pathophysiological bone resorption is commonly associated with periodontal disease and involves the excessive resorption of bone matrix by activated osteoclasts. Receptor activator of nuclear factor (NF)-κB ligand (RANKL) signaling pathways have been proposed as targets for inhibiting osteoclast differentiation and bone resorption. The fungal secondary metabolite (+)-terrein is a natural compound derived from Aspergillus terreus that has previously shown anti-interleukin-6 properties related to inflammatory bone resorption. However, its effects and molecular mechanism of action on osteoclastogenesis and bone resorption remain unclear. In the present study, we showed that 10 µM synthetic (+)-terrein inhibited RANKL-induced osteoclast formation and bone resorption in a dose-dependent manner and without cytotoxicity. RANKL-induced messenger RNA expression of osteoclast-specific markers including nuclear factor of activated T-cells cytoplasmic 1 (NFATc1), the master regulator of osteoclastogenesis, cathepsin K, tartrate-resistant acid phosphatase (Trap) was completely inhibited by synthetic (+)-terrein treatment. Furthermore, synthetic (+)-terrein decreased RANKL-induced NFATc1 protein expression. This study revealed that synthetic (+)-terrein attenuated osteoclast formation and bone resorption by mediating RANKL signaling pathways, especially NFATc1, and indicated the potential effect of (+)-terrein on inflammatory bone resorption including periodontal disease.

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  • Evaluation of the simulator with automatic irrigation control system designed for countermeasures of internal contamination in dental unit water lines. 査読 国際誌

    Keisuke Okubo, Takashi Ito, Kentaro Okamoto, Ichiro Yamamoto, Hajime Mizutani, Yusuke Kawata, Yasuyoshi Shiota, Masahiro Ito, Shin Nakamura, Masako Tai, Tadashi Yamamoto, Shogo Takashiba

    Heliyon   6 ( 6 )   e04132   2020年6月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The prevention of nosocomial infections is an imperative task. The dental chair unit (DCU) is an indispensable device used in dental treatment. However, it is known that the dental unit water line (DUWL) can become contaminated with biofilm, consisting mainly of heterotrophic bacteria (HB). Recently, the International Organization for Standardization specified the methods for testing DUWL contamination management. On these grounds, a simulator reproducing DUWL was prepared to standardize the examination method of the DUWL contamination. Objectives: To evaluate the reproducibility of the DUWL simulator, monitor the DUWL contamination states, and test the efficacy of a commercial decontaminant for DUWL. Methods: The DUWL simulator was assembled by a DCU manufacturing company. The simulator's DUWL was filled with tap water (TW), and left for approximately one year. Neutral electrolyzed water (NEW) was used as a decontaminant for DUWL. Both TW and NEW were passed through DUWL in a timely manner simulating daily dental treatment. Water was sampled from the air turbine hand piece weekly for 4 weeks and used for HB culture. Contamination status was evaluated by measuring bacterial adenosine triphosphate release and by culturing on Reasoner's 2A medium. Results: The DUWL released contaminated water had a bacterial count of over 6 × 104 cfu/mL. After passing NEW through DUWL for 1 week, the count drastically decreased to its basal level and remained steady for 4 weeks. However, TW showed no effect on DUWL decontamination throughout the examination periods. Conclusions: The DUWL simulator could be useful to examine the efficacy of the decontaminant for DUWL and development of new methods in DUWL contamination management.

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  • Graphene oxide: A new direction in dentistry 査読 国際誌

    Mohammed Zahedul Islam Nizami, Shogo Takashiba, Yuta Nishina

    Applied Materials Today   19   100576   2020年6月

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    掲載種別:研究論文(学術雑誌)  

    This inclusive review summarizes the recent advances in the application of graphene oxide (GO) and functionalized GO in oral and dental research. GO possesses several extraordinary physical, chemical, optical, electrical, and mechanical properties. Because of its high surface area and oxygenated functional groups, GO exhibits excellent interaction ability with metals and ions as well as organic species. The current review reveals that GO has been used to produce a variety of functionalized nanocomposites, scaffolds, and advanced nanoparticle carriers. Accordingly, GO shows potential in a variety of research fields, such as tissue engineering, materials engineering, biomaterials, and drug delivery, indicating that the application of GO to biomedicine is particularly promising. More specifically, the recent application of GO in dentistry has provided outstanding results in antimicrobial action, regenerative dentistry, bone tissue engineering, drug delivery, physicomechanical property enhancement of dental biomaterials, and oral cancer treatment. The biocompatibilities of GO and its nanocomposites make them potential units in bone regeneration, osseointegration, and cell proliferation. Furthermore, its antibiofilm and antiadhesion properties have inspired researchers to develop GO for biofilm and caries prevention, as well as implant surface modification and as a quorum sensing inhibitor. This updated review is wide-ranging and provides a useful source for additional information on GO and its composites in dental research and applications.

    DOI: 10.1016/j.apmt.2020.100576

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  • Tailoring the interaction between graphene oxide and antibacterial pyridinium salts by terminal functional groups 査読 国際誌

    R. Fujii, K. Okubo, S. Takashiba, A. Bianco, Y. Nishina

    Carbon   160   204 - 210   2020年4月

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    掲載種別:研究論文(学術雑誌)  

    Nanocarbons, especially two-dimensional carbons, have received considerable attention due to their unique structure and physical and chemical properties, which make them promising candidate materials for biomedical applications. In this study, we focus on graphene oxide (GO), which has many oxygenated functional groups and high affinity with water and biomaterials, and the synthesis of GO complexes with antibacterial agents, like cetylpyridinium chloride (CPC) and its derivatives. We found that the sustained release of CPCs from GO can be controlled by changing the terminal functional group of CPC. The prepared GO-CPC complexes were subjected to antibacterial tests against S. mutans. CPC with the carboxy group was degraded by the oxidizing property of GO, resulting in the loss of antibacterial properties. On the other hand, the other CPC derivatives were released from GO and showed antibacterial activities. Finally, we propose a new mechanism describing how GO and CPC form a functional complex, and how CPC is released from this complex. These findings will lead to pioneering the carbon-based functional antibacterial agents designed at the molecular level.

    DOI: 10.1016/j.carbon.2019.11.094

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  • Efficacy and safety of PERIOdontal treatment versus usual care for Nonalcoholic liver disease: protocol of the PERION multicenter, two-arm, open-label, randomized trial. 査読 国際誌

    Yohei Kamata, Takaomi Kessoku, Tomoko Shimizu, Takashi Kobayashi, Takeo Kurihashi, Satsuki Sato, Syotaro Kuraji, Norio Aoyama, Tomoyuki Iwasaki, Shogo Takashiba, Nobushiro Hamada, Toshiro Kodama, Toshiyuki Tamura, Satoshi Ino, Takuma Higurashi, Masataka Taguri, Takeharu Yamanaka, Masato Yoneda, Haruki Usuda, Koichiro Wada, Atsushi Nakajima, Masato Minabe

    Trials   21 ( 1 )   291 - 291   2020年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: We report the first protocol for a multicenter, randomized comparison study to compare the efficacies of periodontal scaling and root-planing treatment against that of tooth-brushing treatment for nonalcoholic fatty liver disease (NAFLD) (PERION: PERIOdontal treatment for NAFLD). Nonalcoholic steatohepatitis (NASH) is an advanced form of NAFLD, which can progress to cirrhosis and hepatocellular carcinoma. Increased endotoxemia is associated with the progression of NAFLD. Periodontal bacteria possess endotoxins; Porphyromonas gingivalis is well-known as a major pathogenic bacterium in periodontitis, and serum antibody levels for P. gingivalis are high in patients with periodontitis. Several reports have indicated that P. gingivalis is related to NAFLD. This study aims to investigate the effect of periodontal treatment for liver damage, P. gingivalis infection, and endotoxemia on patients with NAFLD. METHODS: We will include adult patients (20-85 years old) with NAFLD, alanine aminotransferase (ALT) ≥ 40 IU/L, and equivalent steatosis grade ≥ 1 (target sample size, n = 40 patients; planned number of patients with outcome data, n = 32). Participants will be randomly assigned to one of two groups: a scaling and root-planing group or tooth-brushing as the usual group. The primary outcome will be the change in ALT levels from baseline to 12 weeks; the key secondary outcome will be the change in the serum immunoglobulin G (IgG) antibody titer for P. gingivalis at 12 weeks. DISCUSSION: This study should determine whether periodontal treatment decreases liver damage, P. gingivalis infection, and endotoxemia in patients with NAFLD. TRIAL REGISTRATION: University Hospital Medical Information Network (UMIN) Clinical Trials Registry, ID: UMIN000022079.

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  • Antimicrobial and antibiofilm effects of abietic acid on cariogenic Streptococcus mutans. 査読 国際誌

    Yuki Ito, Takashi Ito, Keisuke Yamashiro, Fumi Mineshiba, Kimito Hirai, Kazuhiro Omori, Tadashi Yamamoto, Shogo Takashiba

    Odontology   108 ( 1 )   57 - 65   2020年1月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Dental caries is a type of oral microbiome dysbiosis and biofilm infection that affects oral and systemic conditions. For healthy life expectancy, natural bacteriostatic products are ideal for daily and lifetime use as anti-oral infection agents. This study aimed to evaluate the inhibitory effects of abietic acid, a diterpene derived from pine rosin, on the in vitro growth of cariogenic bacterial species, Streptococcus mutans. The effective minimum inhibitory concentration of abietic acid was determined through observation of S. mutans growth, acidification, and biofilm formation. The inhibitory effects of abietic acid on the bacterial membrane were investigated through the use of in situ viability analysis and scanning electron microscopic analysis. Cytotoxicity of abietic acid was also examined in the context of several human cell lines using tetrazolium reduction assay. Abietic acid was found to inhibit key bacterial growth hallmarks such as colony forming ability, adenosine triphosphate activity (both planktonic and biofilm), acid production, and biofilm formation. Abietic acid was identified as bacteriostatic, and this compound caused minimal damage to the bacterial membrane. This action was different from that of povidone-iodine or cetylpyridinium chloride. Additionally, abietic acid was significantly less cytotoxic compared to povidone-iodine, and it exerted lower toxicity towards epithelial cells and fibroblasts compared to that against monocytic cells. These data suggest that abietic acid may prove useful as an antibacterial and antibiofilm agent for controlling S. mutans infection.

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  • High Mobility Group Box 1 Expression in Oral Inflammation and Regeneration. 査読 国際誌

    Keisuke Yamashiro, Hidetaka Ideguchi, Hiroaki Aoyagi, Chiaki Yoshihara-Hirata, Anna Hirai, Risa Suzuki-Kyoshima, Yao Zhang, Hidenori Wake, Masahiro Nishibori, Tadashi Yamamoto, Shogo Takashiba

    Frontiers in immunology   11   1461 - 1461   2020年

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    担当区分:最終著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    High mobility group box 1 (HMGB1) is a non-histone DNA-binding protein of about 30 kDa. It is released from a variety of cells into the extracellular milieu in response to inflammatory stimuli and acts on specific cell-surface receptors, such as receptors for advanced glycation end-products (RAGE), Toll-like receptor (TLR)2, TLR4, with or without forming a complex with other molecules. HMGB1 mediates various mechanisms such as inflammation, cell migration, proliferation, and differentiation. On the other hand, HMGB1 enhances chemotaxis acting through the C-X-C motif chemokine ligand (CXCL)12/C-X-C chemokine receptor (CXCR)4 axis and is involved in regeneration. In the oral cavity, high levels of HMGB1 have been detected in the gingival tissue from periodontitis and peri-implantitis patients, and it has been shown that secreted HMGB1 induces pro-inflammatory cytokine expression, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α, which prolong inflammation. In contrast, wound healing after tooth extraction or titanium dental implant osseointegration requires an initial acute inflammation, which is regulated by secreted HMGB1. This indicates that secreted HMGB1 regulates angiogenesis and bone remodeling by osteoclast and osteoblast activation and promotes bone healing in oral tissue repair. Therefore, HMGB1 can prolong inflammation in the periodontal tissue and, conversely, can regenerate or repair damaged tissues in the oral cavity. In this review, we highlight the role of HMGB1 in the oral cavity by comparing its function and regulation with its function in other diseases. We also discuss the necessity for further studies in this field to provide more specific scientific evidence for dentistry.

    DOI: 10.3389/fimmu.2020.01461

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  • Acute Prevertebral Abscesses Caused by Bacterial-infected Traumatic Tooth Fractures. 査読 国際誌

    Kazuyuki Matsunaga, Makoto Takemaru, Keisuke Yamashiro, Chiaki Yoshihara-Hirata, Ken Inohara, Yutaka Shimoe, Akio Tanaka, Masaru Kuriyama, Shogo Takashiba

    Acta medica Okayama   73 ( 5 )   449 - 456   2019年10月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We report a case of acute prevertebral abscess caused by traumatic tooth fractures in a 77-year-old Japanese man. After being transferred to our hospital the patient was initially diagnosed with a neck hematoma; however, blood culture showed Streptococcus parasanguinis, an oral bacterium, and an MRI examination suggested prevertebral abscesses. Tooth fractures, severe periodontitis, and peri-implantitis with Streptococcus parasanguinis were observed. Antibiotics were administered and fractured teeth were extracted. The patient's condition then gradually improved. We concluded that bacteremia caused by traumatic tooth fractures induced the acute prevertebral abscesses.

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  • Construction and characterization of a PGN_0297 mutant of Porphyromonas gingivalis: evidence of the contribution of PGN_0297 to gingipain activity. 査読 国際誌

    Ono S, Nakayama M, Tachibana M, Shahriar ASM, Heling W, Takashiba S, Ohara N

    Acta Medica Okayama   73 ( 4 )   315 - 323   2019年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.18926/AMO/56933

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  • Effectiveness and safety of low-concentrated ozonized water for the reduction of contamination in dental unit water lines. 査読 国際誌

    Keisuke Okubo, Takashi Ito, Yasuyoshi Shiota, Yusuke Kawata, Tadashi Yamamoto, Shogo Takashiba

    Heliyon   5 ( 8 )   e02306   2019年8月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Contamination of dental unit waterlines (DUWL) with heterotrophic bacteria can cause problems in immune compromised patients (aged, tumor and organ transplantation-patients). We focused on the use of low-concentrated ozonized water (OZW) as the biofilm formation restraint system for DUWL. Here, we examined the effects of low-concentrated OZW on the growth of bacteria and related biofilm formation and harmfulness to dental unit components (DUCs) in vitro. Objectives: To evaluate the bactericidal effects of OZW on biofilms in DUWL and DUC in vitro. Methods: Low-concentrated OZW (0.4 mg/L) was generated using an OZS-PTDX generator. Heterotrophic bacterial biofilms in old DUWL tubes and Candia albicans solution (control microbe) were treated with OZW for 1 h with gentle agitation before static culturing for 96 h in Reasoner's 2A liquid media. The control solutions were 0.1% cetylpyridinium chloride (CPC), chlorinated tap water (TW), and phosphate-buffered saline (PBS). Adenosine triphosphate (ATP) amounts of the microbes were measured and the biofilms of these microbes were observed using scanning electron microscopy (SEM). Moreover, surfaces of DUC soaked in OZW and TW were observed by SEM. Results: The OZW reduced ATP levels in microbes to 50% compared to TW and PBS treatment, although CPC reduced it below detection limits. SEM observation revealed deformation of microbes cultured with OZW, whereas no changes were seen on DUC surfaces. Conclusions: Low-concentrated OZW is bactericidal against heterotrophic bacteria biofilms and it is not harmful to DUC, suggesting that it might be useful in preventing DUWL contamination.

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  • Multidisciplinary clinical approach by sharing oral examination information to treat a diabetes patient with dysgeusia. 査読 国際誌

    Kazuyuki Matsunaga, Yasuko Yoshida, Makoto Takemaru, Keisuke Yamashiro, Ikuko Monden, Ken Inohara, Saki Nakagawa, Eriko Maeda, Kanako Nakahama, Tatsuo Kohriyama, Shogo Takashiba

    Clinical case reports   7 ( 5 )   877 - 880   2019年5月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Taste alteration is one of the complications of severe diabetes. It is important in diabetes treatment to assess taste alteration and perform dietary counseling, therapeutic exercise, and oral care. In this case, multidisciplinary clinical approach by medical staff was successful for a severely diabetic patient with dysgeusia.

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  • Molecular imaging assessment of periodontitis lesions in an experimental mouse model 査読 国際誌

    Hidetaka Ideguchi, Keisuke Yamashiro, Tadashi Yamamoto, Masayuki Shimoe, Shoichi Hongo, Shinsuke Kochi, Chiaki Yoshihara-Hirata, Hiroaki Aoyagi, Mari Kawamura, Shogo Takashiba

    Clinical Oral Investigations   23 ( 2 )   821 - 827   2019年2月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Verlag  

    Objective: We aimed to evaluate molecular imaging as a novel diagnostic tool for mice periodontitis model induced by ligature and Porphyromonas gingivalis (Pg) inoculation. Materials and methods: Twelve female mice were assigned to the following groups: no treatment as control group (n = 4)
    periodontitis group induced by ligature and Pg as Pg group (n = 4)
    and Pg group treated with glycyrrhizinic acid (GA) as Pg + GA group (n = 4). All mice were administered a myeloperoxidase (MPO) activity-specific luminescent probe and observed using a charge-coupled device camera on day 14. Image analysis on all mice was conducted using software to determine the signal intensity of inflammation. Additionally, histological and radiographic evaluation for periodontal inflammation and bone resorption at the site of periodontitis, and quantitative enzyme-linked immunosorbent assay (ELISA) were conducted on three mice for each group. Each experiment was performed three times. Results: Levels of serum IgG antibody against P. gingivalis were significantly higher in the Pg than in the Pg + GA group. Histological analyses indicated that the number of osteoclasts and neutrophils were significantly lower in the Pg + GA than in the Pg group. Micro-CT image analysis indicated no difference in bone resorption between the Pg and Pg + GA groups. The signal intensity of MPO activity was detected on the complete craniofacial image
    moreover, strong signal intensity was localized specifically at the periodontitis site in the ex vivo palate, with group-wise differences. Conclusions: Molecular imaging analysis based on MPO activity showed high sensitivity of detection of periodontal inflammation in mice. Clinical relevance: Molecular imaging analysis based on MPO activity has potential as a diagnostic tool for periodontitis.

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  • Induction of migration of periodontal ligament cells by selective regulation of integrin subunits. 査読 国際誌

    Mari Kawamura, Tadashi Yamamoto, Keisuke Yamashiro, Shinsuke Kochi, Chiaki Yoshihara-Hirata, Hidetaka Ideguchi, Hiroaki Aoyagi, Kazuhiro Omori, Shogo Takashiba

    Journal of cellular and molecular medicine   23 ( 2 )   1211 - 1223   2019年2月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The recruitment of tissue-resident stem cells is important for wound regeneration. Periodontal ligament cells (PDL cells) are heterogeneous cell populations with stemness features that migrate into wound sites to regenerate periodontal fibres and neighbouring hard tissues. Cell migration is regulated by the local microenvironment, coordinated by growth factors and the extracellular matrix (ECM). Integrin-mediated cell adhesion to the ECM provides essential signals for migration. We hypothesized that PDL cell migration could be enhanced by selective expression of integrins. The migration of primary cultured PDL cells was induced by platelet-derived growth factor-BB (PDGF-BB). The effects of blocking specific integrins on migration and ECM adhesion were investigated based on the integrin expression profiles observed during migration. Up-regulation of integrins α3, α5, and fibronectin was identified at distinct localizations in migrating PDL cells. Treatment with anti-integrin α5 antibodies inhibited PDL cell migration. Treatment with anti-integrin α3, α3-blocking peptide, and α3 siRNA significantly enhanced cell migration, comparable to treatment with PDGF-BB. Furthermore, integrin α3 inhibition preferentially enhanced adhesion to fibronectin via integrin α5. These findings indicate that PDL cell migration is reciprocally regulated by integrin α3-mediated inhibition and α5-mediated promotion. Thus, targeting integrin expression is a possible therapeutic strategy for periodontal regeneration.

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  • Involvement of PM2.5-bound protein and metals in PM2.5-induced allergic airway inflammation in mice 査読

    Keiki Ogino, Kenjiro Nagaoka, Tatsuo Ito, Kei Takemoto, Tomoaki Okuda, Shoji F. Nakayama, Noriyoshi Ogino, Yuka Seki, Hiroki Hamada, Shogo Takashiba, Yoshihisa Fujikura

    Inhalation Toxicology   30 ( 13-14 )   498 - 508   2018年12月

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    掲載種別:研究論文(学術雑誌)  

    Background: The aim of this study was to investigate the protein and trace element components of PM2.5 and their contribution to the allergic airway inflammation in BALB/c mice. Methods: PM2.5, treated at high temperature and with a strong acid to hydrolyze any protein content and remove trace elements, was administered to BALB/c mice. Allergic airway inflammation was compared between the three groups (saline, pure PM2.5 and treated PM2.5) by evaluating airway hyperresponsiveness (AHR), bronchoalveolar lavage fluid (BALF) cells, serum IgE, the mRNA of various cytokine (IL-4, IL-5, IL-13, eotaxin-1 and CXCL3), mucus protein mRNA (MUC5ac and MUC5b) and the filtration of inflammatory cells in the lung. Results: The treatment of PM2.5 with a strong acid at a high temperature attenuated AHR, eosinophil percentage in BALF, mRNA levels of IL-13 and CXCL3 and peribronchial inflammation. On the contrary, the percentage of neutrophils in BALF, mRNA expression of MIP2α, EGFR, Nrf2, and TLR4 and 4-OH-2-nonenal levels in the lung was increased. Moreover, the treatment of the PM2.5 reduced PM2.5-bound proteins as well as the percentages of the trace elements in PM2.5 in the order Zn > Cu > Pb > P > S > Mn > Fe > Ca > Ni, whereas the percentage of C, Si and Cl increased. Conclusions: PM2.5 collected by of the cyclone system induced allergic airway inflammation in mice. PM2.5-bound proteins and acid-soluble metals may be involved in the pathogenesis of PM2.5-induced allergic airway inflammation.

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  • Fungal metabolite (+)-terrein suppresses IL-6/sIL-6R-induced CSF1 secretion by inhibiting JAK1 phosphorylation in human gingival fibroblasts. 査読 国際誌

    Satoshi Yamamoto, Kazuhiro Omori, Hiroki Mandai, Masaaki Nakayama, Saki Nakagawa, Hiroya Kobayashi, Tadashi Kunimine, Hiroshi Yoshimura, Kyosuke Sakaida, Hidefumi Sako, Soichiro Ibaragi, Tadashi Yamamoto, Hiroshi Maeda, Seiji Suga, Shogo Takashiba

    Heliyon   4 ( 11 )   e00979 - e00979   2018年11月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    Control of bacterial infection-induced inflammatory responses is one of the effective therapeutic approaches of periodontal diseases. Natural products such as lipid mediators and metabolites from microorganisms have been used for decreasing inflammation. We previously reported that (+)-terrein inhibited activation of STAT3 and ERK1/2 in interleukin-6 (IL-6) signaling cascade, leading to prevent vascular endothelial growth factor (VEGF) secretion in human gingival fibroblasts (HGFs). However, little is still known about the role of (+)-terrein on inflammatory responses. In this study, we provided the possibility of novel action that (+)-terrein inhibits activation of Janus-activated kinase 1 (JAK1), which has a central function in IL-6 signaling cascade, and alters expression of mRNAs and proteins induced by IL-6/soluble IL-6 receptor (sIL-6R) stimulation in HGFs. First, we performed PCR array to examine IL-6/sIL-6R-induced mRNA expression, and then expression of mRNA and protein of colony stimulating factor-1 (CSF1) and VEGF were clearly determined by quantitative RT-PCR and ELISA, respectively. Treatment with (+)-terrein suppressed expression of mRNA and protein of CSF1 and VEGF by IL-6/sIL-6R stimulation. Next, to test the effect of (+)-terrein on IL-6/sIL-6R signaling cascade, we demonstrated whether (+)-terrein affects phosphorylation of JAK1 and its downstream proteins, Akt and SHP-2. Western blotting revealed that (+)-terrein inhibited IL-6/sIL-6R-induced phosphorylation of JAK1, Akt, and SHP-2. Therefore, (+)-terrein suppresses IL-6/sIL-6R-induced expression of CSF1 and VEGF via inhibition of JAK1, Akt, and SHP-2. Based on our results, we suggest that (+)-terrein is a candidate compound for anti-inflammatory effect associated with IL-6 signaling.

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  • HMGB1-induced inflammatory response promotes bone healing in murine tooth extraction socket 査読 国際誌

    Hiroaki Aoyagi, Keisuke Yamashiro, Chiaki Hirata-Yoshihara, Hidetaka Ideguchi, Mutsuyo Yamasaki, Mari Kawamura, Tadashi Yamamoto, Shinsuke Kochi, Hidenori Wake, Masahiro Nishibori, Shogo Takashiba

    Journal of Cellular Biochemistry   119 ( 7 )   5481 - 5490   2018年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley-Liss Inc.  

    High mobility group box 1 (HMGB1) is a non-histone DNA-binding protein that is secreted into the extracellular milieu in response to inflammatory stimuli. The secreted HMGB1 has been suggested to mediate various inflammatory diseases. However, it is still unknown whether HMGB1 is involved in a healing process in the tooth extraction socket, the tissue containing gingival epithelium, and alveolar bone that is exposed to oral bacteria. In this study, we constructed a murine tooth extraction model with anti-HMGB1 neutralization antibody administration and observed the inflammatory response and bone healing process in tooth extraction sockets by molecular imaging of myeloperoxidase (MPO) activity, histological analysis, and quantitative RT-PCR. The translocation of HMGB1 from the nucleus to the cytoplasm in gingival epithelial cells and inflammatory cells was inhibited by anti-HMGB1 antibody administration. The MPO activity around the tooth extraction socket was significantly reduced, and the numbers of CD31- and CD68-positive cells were significantly lower in the anti-HMGB1 antibody treatment samples than in the control samples. The TRAP-positive cells, osteocalcin positive cells, and the neoplastic bone area were significantly lower in anti-HMGB1 antibody treatment samples than in control samples. The expression levels of IL-1β and VEGF-A were also decreased in anti-HMGB1 antibody treatment samples compared to that in control samples. Secreted HMGB1 induced initial acute inflammation and inflammatory cells recruitment after tooth extraction. HMGB1 was associated with angiogenesis and bone remodeling by osteoclast and osteoblast activation and promoted bone healing in the tooth extraction socket.

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  • Effects of periodontal treatment on carotid intima-media thickness in patients with lifestyle-related diseases: Japanese prospective multicentre observational study 査読

    Chieko Kudo, Wee Soo Shin, Nobuhiro Sasaki, Kazuo Harai, Kai Kato, Hiroaki Seino, Eiji Goke, Takemasa Fujino, Nobuichi Kuribayashi, Youko Onuki Pearce, Masato Taira, Ryoji Matsushima, Masato Minabe, Shogo Takashiba, Kiyotaka Fujii, Tooru Hanai, Kouya Honda, Yoshichika Horiuchi, Hiroshi Inoue, Kiyoo Ishige, Shinichi Itoh, Sachiho Iwatsuka, Tomoko Kakugawa, Hideto Komai, Chikara Morimoto, Mitsutaka Motoyoshi, Masato Nakamura, Mikiko Niida, Ryuji Numaguchi, Kiyomi Oono, Hidetoshi Sakai, Yasunari Sakomura, Takaaki Shinohara, Yuichi Takakaze, Yukio Tsuruta, Daigaku Uchida, Norihide Ueno, Osamu Yasuda, Periodontitis and Atherosclerosis Project-Tokyo and Chiba Consortiums

    Odontology   106 ( 3 )   349 - 349   2018年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Tokyo  

    Unfortunately, in Table-5 of the original article, the parameter in the 5th row was published incorrectly as “LDL-C (mg/dL)”. The correct parameter should read as “HDL-C (mg/dL)”.

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  • Effects of periodontal treatment on carotid intima-media thickness in patients with lifestyle-related diseases: Japanese prospective multicentre observational study 査読

    Chieko Kudo, Wee Soo Shin, Nobuhiro Sasaki, Kazuo Harai, Kai Kato, Hiroaki Seino, Eiji Goke, Takemasa Fujino, Nobuichi Kuribayashi, Youko Onuki Pearce, Masato Taira, Ryoji Matsushima, Masato Minabe, Shogo Takashiba, Kiyotaka Fujii, Tooru Hanai, Kouya Honda, Yoshichika Horiuchi, Hiroshi Inoue, Kiyoo Ishige, Shinichi Itoh, Sachiho Iwatsuka, Tomoko Kakugawa, Hideto Komai, Chikara Morimoto, Mitsutaka Motoyoshi, Masato Nakamura, Mikiko Niida, Ryuji Numaguchi, Kiyomi Oono, Hidetoshi Sakai, Yasunari Sakomura, Takaaki Shinohara, Yuichi Takakaze, Yukio Tsuruta, Daigaku Uchida, Norihide Ueno, Osamu Yasuda, Periodontitis and Atherosclerosis Project-Tokyo and Chiba Consortiums

    Odontology   106 ( 3 )   349 - 327   2018年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Tokyo  

    Unfortunately, in Table-5 of the original article, the parameter in the 5th row was published incorrectly as “LDL-C (mg/dL)”. The correct parameter should read as “HDL-C (mg/dL)”.

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  • Anti-HMGB1 neutralizing antibody attenuates periodontal inflammation and bone resorption in a murine periodontitis model 査読 国際誌

    Chiaki Yoshihara-Hirata, Keisuke Yamashiro, Tadashi Yamamoto, Hiroaki Aoyagi, Hidetaka Ideguchi, Mari Kawamura, Risa Suzuki, Mitsuaki Ono, Hidenori Wake, Masahiro Nishibori, Shogo Takashiba

    Infection and Immunity   86 ( 5 )   2018年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    High mobility group box 1 (HMGB1) is a non-histone DNA-binding protein that is secreted into the extracellular milieu in response to inflammatory stimuli. The secreted HMGB1 mediates various inflammatory diseases, including periodontitis
    however, the underlying mechanisms of HMGB1-induced periodontal inflammation are not completely understood. Here, we examined whether anti-HMGB1 neutralizing antibody inhibits periodontal progression and investigated the molecular pathology of HMGB1 in vitro and in vivo. In vitro analysis indicated that HMGB1, granulocytemacrophage colony-stimulating factor (GM-CSF), and interleukin-1β (IL-1β) were secreted in response to tumor necrosis factor-α (TNF-α) stimuli in human gingival epithelial cells (HGECs) and human monocytic leukemia cells (THP-1) treated with phorbol myristate acetate. Increased levels of GM-CSF and IL-1β were observed in the conditioned media from TNF-α-stimulated HGECs and THP-1 in vitro. Simultaneous stimulation with TNF-α and anti-HMGB1 antibody significantly decreased TNF-α- induced inflammatory cytokine secretion. Experimental periodontitis was induced in mice using Porphyromonas gingivalis-soaked ligatures. The extracellular translocation was confirmed in gingival epithelia in the periodontitis model mice by immunofluorescence analysis. Systemic administration of anti-HMGB1 neutralizing antibody significantly inhibited translocation of HMGB1. The anti-HMGB1 antibody inhibited periodontal inflammation, expression of IL-1β and C-X-C motif chemokine ligand 1 (CXCL1), migration of neutrophils, and bone resorption, shown by bioluminescence imaging of myeloperoxidase activity, quantitative reverse transcription-PCR (RT-PCR), and micro-computed tomography analysis. These findings indicate that HMGB1 is secreted in response to inflammatory stimuli caused by periodontal infection, which is crucial for the initiation of periodontitis, and the anti-HMGB1 antibody attenuates the secretion of a series of inflammatory cytokines, consequently suppressing the progression of periodontitis.

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  • Correction to: Expression of optineurin isolated from rat-injured dental pulp and the effects on inflammatory signals in normal rat kidney cells (Odontology, (2018), 106, 2, (135-144), 10.1007/s10266-017-0314-5) 査読

    Kyoko Senoo, Keisuke Yamashiro, Tadashi Yamamoto, Fumio Myokai, Mari Kawamura, Shogo Takashiba

    Odontology   106 ( 2 )   223 - 223   2018年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Tokyo  

    In the original publication of the article, one of the author name was published incorrectly as “Keisuke Yamashairo” and correct name should be “Keisuke Yamashiro”.

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  • Expression of optineurin isolated from rat-injured dental pulp and the effects on inflammatory signals in normal rat kidney cells 査読 国際誌

    Senoo K, Yamashiro K, Yamamoto T, Myokai F, Kawamura M, Takashiba S

    Odontology   106 ( 2 )   135 - 144   2018年4月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

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  • Modulation of microenvironment for controlling the fate of periodontal ligament cells: the role of Rho/ROCK signaling and cytoskeletal dynamics 査読 国際誌

    Tadashi Yamamoto, Yuki Ugawa, Mari Kawamura, Keisuke Yamashiro, Shinsuke Kochi, Hidetaka Ideguchi, Shogo Takashiba

    Journal of Cell Communication and Signaling   12 ( 1 )   369 - 378   2018年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Netherlands  

    Cells behave in a variety of ways when they perceive changes in their microenvironment
    the behavior of cells is guided by their coordinated interactions with growth factors, niche cells, and extracellular matrix (ECM). Modulation of the microenvironment affects the cell morphology and multiple gene expressions. Rho/Rho-associated coiled-coil-containing protein kinase (ROCK) signaling is one of the key regulators of cytoskeletal dynamics and actively and/or passively determines the cell fate, such as proliferation, migration, differentiation, and apoptosis, by reciprocal communication with the microenvironment. During periodontal wound healing, it is important to recruit the residential stem cells into the defect site for regeneration and homeostasis of the periodontal tissue. Periodontal ligament (PDL) cells contain a heterogeneous fibroblast population, including mesenchymal stem cells, and contribute to the reconstruction of tooth-supporting tissues. Therefore, bio-regeneration of PDL cells has been the ultimate goal of periodontal therapy for decades. Recent stem cell researches have shed light on intrinsic ECM properties, providing paradigm shifts in cell fate determination. This review focuses on the role of ROCK activity and the effects of Y-27632, a specific inhibitor of ROCK, in the modulation of ECM-microenvironment. Further, it presents the current understanding of how Rho/ROCK signaling affects the fate determination of stem cells, especially PDL cells. In addition, we have also discussed in detail the underlying mechanisms behind the reciprocal response to the microenvironment.

    DOI: 10.1007/s12079-017-0425-3

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  • The KCNQ1 gene polymorphism as a shared genetic risk for rheumatoid arthritis and chronic periodontitis in Japanese adults: A pilot case-control study. 査読 国際誌

    Kobayashi T, Kido JI, Ishihara Y, Omori K, Ito S, Matsuura T, Bando T, Wada J, Murasawa A, Nakazono K, Mitani A, Takashiba S, Nagata T, Yoshie H

    Journal of periodontology   89 ( 3 )   315 - 324   2018年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1002/JPER.17-0412

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  • Effects of Lectins on initial attachment of cariogenic Streptococcus mutans 査読 国際誌

    Takashi Ito, Yasuhiro Yoshida, Yasuyoshi Shiota, Yuki Ito, Tadashi Yamamoto, Shogo Takashiba

    Glycoconjugate Journal   35 ( 1 )   41 - 51   2018年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer New York LLC  

    Oral bacteria initiate biofilm formation by attaching to tooth surfaces via an interaction of a lectin-like bacterial protein with carbohydrate chains on the pellicle. This study aimed to find naturally derived lectins that inhibit the initial attachment of a cariogenic bacterial species, Streptococcus mutans (S. mutans), to carbohydrate chains in saliva in vitro. Seventy kinds of lectins were screened for candidate motifs that inhibit the attachment of S. mutans ATCC 25175 to a saliva-coated culture plate. The inhibitory effect of the lectins on attachment of the S. mutans to the plates was quantified by crystal violet staining, and the biofilm was observed under a scanning electron microscope (SEM). Surface plasmon resonance (SPR) analysis was performed to examine the binding of S. mutans to carbohydrate chains and the binding of candidate lectins to carbohydrate chains, respectively. Moreover, binding assay between the biotinylated-lectins and the saliva components was conducted to measure the lectin binding. Lectins recognizing a salivary carbohydrate chain, Galβ1-3GalNAc, inhibited the binding of S. mutans to the plate. In particular, Agaricus bisporus agglutinin (ABA) markedly inhibited the binding. This inhibition was confirmed by SEM observation. SPR analysis indicated that S. mutans strongly binds to Galβ1-3GalNAc, and ABA binds to Galβ1-3GalNAc. Finally, the biotinylated Galβ1-3GalNAc-binding lectins including ABA demonstrated marked binding to the saliva components. These results suggest that ABA lectin inhibited the attachment of S. mutans to Galβ1-3GalNAc in saliva and ABA can be useful as a potent inhibitor for initial attachment of oral bacteria and biofilm formation.

    DOI: 10.1007/s10719-017-9795-2

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  • IS1598 (IsPg4) distributed to abscess-forming strains of Porphyromonas gingivalis may enhance virulence through upregulation of nrdD-like gene expression 査読 国際誌

    Norihiro Sonoi, Hiroshi Maeda, Toshimitsu Murauchi, Tadashi Yamamoto, Kazuhiro Omori, Susumu Kokeguchi, Koji Naruishi, Shogo Takashiba

    New Microbiologica   41 ( 1 )   52 - 60   2018年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Luigi Ponzio e figlio Editori  

    An insertion sequence, IS1598 (IsPg4) has been found in virulent strains of Porphyromonas gingivalis in a murine abscess model. The present study was performed to investigate the effects of genetic rearrangements by IS1598 on the phenotypic characteristics of the virulent strains. For this purpose, we searched for a common insertion site of IS1598 among the virulent strains. Through cloning and database search, a common insertion site was identified beside an nrdD-like gene in the virulent FDC 381, W83 and W50 strains. In this region, predicted promoters of the nrdD-like gene and IS1598 are located in tandem, and accumulation of nrdD-like gene mRNA was 5-fold higher in virulent strains (W83, W50, FDC 381) than avirulent strains (ATCC33277, SU63, SUNY1021, ESO59 without IS1598). The role of the nrdD-like gene in virulence of P. gingivalis was investigated by constructing a nrdD-deficient mutant. In the murine abscess model, the parental W83 strain produced necrotic abscesses, while the nrdD-deficient mutant had almost lost this ability. Insertion of IS1598 into the nrdD-like gene promoter region may be related to the phenotypic differences in virulence among P. gingivalis strains through upregulation of the expression of this gene.

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  • Regulatory mechanism of CCN2 production by serotonin (5-HT) via 5-HT2A and 5-HT2B receptors in chondrocytes 査読

    Ayaka Hori, Takashi Nishida, Shogo Takashiba, Satoshi Kubota, Masaharu Takigawa

    PLOS ONE   12 ( 11 )   e0188014 - e0188014   2017年11月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Public Library of Science (PLoS)  

    DOI: 10.1371/journal.pone.0188014

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  • Medication-related osteonecrosis of the jaws caused lethal sepsis in an edentulous patient with multiple systemic factors 査読

    Keisuke Yamashiro, Aki Sato, Fumihiko Okazaki, Makoto Nakano, Koichi Sawaki, Yasuhisa Hirata, Eiki Yamachika, Seiji Iida, Shogo Takashiba

    Clinical Case Reports   5 ( 2 )   97 - 103   2017年2月

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    担当区分:最終著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1002/ccr3.751

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  • Assessing the progression of chronic periodontitis using subgingival pathogen levels: a 24-month prospective multicenter cohort study. 査読 国際誌

    E Kakuta, Y Nomura, T Morozumi, T Nakagawa, T Nakamura, K Noguchi, A Yoshimura, Y Hara, O Fujise, F Nishimura, T Kono, M Umeda, M Fukuda, T Noguchi, N Yoshinari, C Fukaya, S Sekino, Y Numabe, N Sugano, K Ito, H Kobayashi, Y Izumi, H Takai, Y Ogata, S Takano, M Minabe, A Makino-Oi, A Saito, Y Abe, S Sato, F Suzuki, K Takahashi, T Sugaya, M Kawanami, N Hanada, S Takashiba, H Yoshie

    BMC oral health   17 ( 1 )   46 - 46   2017年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: The diagnosis of the progression of periodontitis presently depends on the use of clinical symptoms (such as attachment loss) and radiographic imaging. The aim of the multicenter study described here was to evaluate the diagnostic use of the bacterial content of subgingival plaque recovered from the deepest pockets in assessing disease progression in chronic periodontitis patients. METHODS: This study consisted of a 24-month investigation of a total of 163 patients with chronic periodontitis who received trimonthly follow-up care. Subgingival plaque from the deepest pockets was recovered and assessed for bacterial content of Porphyromonas gingivalis, Prevotella intermedia, and Aggregatibacter actinomycetemcomitans using the modified Invader PLUS assay. The corresponding serum IgG titers were measured using ELISA. Changes in clinical parameters were evaluated over the course of 24 months. The sensitivity, specificity, and prediction values were calculated and used to determine cutoff points for prediction of the progression of chronic periodontitis. RESULTS: Of the 124 individuals who completed the 24-month monitoring phase, 62 exhibited progression of periodontitis, whereas 62 demonstrated stable disease. The P. gingivalis counts of subgingival plaque from the deepest pockets was significantly associated with the progression of periodontitis (p < 0.001, positive predictive value = 0.708). CONCLUSIONS: The P. gingivalis counts of subgingival plaque from the deepest pockets may be associated with the progression of periodontitis.

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  • Salivary pathogen and serum antibody to assess the progression of chronic periodontitis: a 24-mo prospective multicenter cohort study 査読

    T. Morozumi, T. Nakagawa, Y. Nomura, T. Sugaya, M. Kawanami, F. Suzuki, K. Takahashi, Y. Abe, S. Sato, A. Makino-Oi, A. Saito, S. Takano, M. Minabe, Y. Nakayama, Y. Ogata, H. Kobayashi, Y. Izumi, N. Sugano, K. Ito, S. Sekino, Y. Numabe, C. Fukaya, N. Yoshinari, M. Fukuda, T. Noguchi, T. Kono, M. Umeda, O. Fujise, F. Nishimura, A. Yoshimura, Y. Hara, T. Nakamura, K. Noguchi, E. Kakuta, N. Hanada, S. Takashiba, H. Yoshie

    Journal of Periodontal Research   51 ( 6 )   768 - 778   2016年12月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/jre.12353

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  • Smad2 overexpression enhances adhesion of gingival epithelial cells 査読

    Shoichi Hongo, Tadashi Yamamoto, Keisuke Yamashiro, Masayuki Shimoe, Kazuya Tomikawa, Yuki Ugawa, Shinsuke Kochi, Hidetaka Ideguchi, Hiroshi Maeda, Shogo Takashiba

    Archives of Oral Biology   71   46 - 53   2016年11月

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    担当区分:最終著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

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  • The potential of positron emission tomography/computerized tomography (PET/CT) scanning as a detector of high-risk patients with oral infection during preoperative staging 査読

    Keisuke Yamashiro, Makoto Nakano, Koichi Sawaki, Fumihiko Okazaki, Yasuhisa Hirata, Shogo Takashiba

    Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology   122 ( 2 )   242 - 249   2016年8月

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    担当区分:最終著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

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  • Effects of new over-the-counter periodontal ointment-containing applicator with single-tuft brush on cytokine levels in gingival crevicular fluid during supportive periodontal therapy phase: a randomized double-blind clinical trial. 査読 国際誌

    K Takeuchi-Hatanaka, T Yasuda, Koji Naruishi, K Katsuragi-Fuke, J Inubushi, H Ootsuki, H Maeda, S Takashiba

    Journal of Periodontal Research   Vol.51 ( No.3 )   321 - 331   2016年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The levels of IL-1β, IL-6, IL-8 and TNF-α remained significantly lower in the test group compared to the placebo group. In the placebo group, when the probing pocket depth at baseline was 4 mm, IL-1β increased, particularly in the second molar tooth, and the greatest increase was seen when PPD at baseline was 5-6 mm. In the test group, IL-1β decreased markedly in cases with furcation involvement and low bleeding on probing at baseline. In both groups, IL-1β, IL-6 and TNF-α were closely correlated with each other.

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  • Synthetic Terrein Inhibits Progression of Head and Neck Cancer by Suppressing Angiogenin Production 査読

    Shibata A, Ibaragi S, Mandai H, Tsumura T, Kishimoto K, Okui T, Hassan NM, Shimo T, Omori K, Hu GF, Takashiba S, Suga S, Sasaki A

    Anticancer Reserach   36 ( 5 )   2161 - 2168   2016年5月

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  • Randomized Placebo‐Controlled and Controlled Non‐Inferiority Phase III Trials Comparing Trafermin, a Recombinant Human Fibroblast Growth Factor 2, and Enamel Matrix Derivative in Periodontal Regeneration in Intrabony Defects 査読

    Masahiro Kitamura, Motoki Akamatsu, Masamitsu Kawanami, Yasushi Furuichi, Takeo Fujii, Mari Mori, Kazushi Kunimatsu, Hidetoshi Shimauchi, Yorimasa Ogata, Matsuo Yamamoto, Taneaki Nakagawa, Shuichi Sato, Koichi Ito, Takefumi Ogasawara, Yuichi Izumi, Kazuhiro Gomi, Kazuhisa Yamazaki, Hiromasa Yoshie, Mitsuo Fukuda, Toshihide Noguchi, Shogo Takashiba, Hidemi Kurihara, Toshihiko Nagata, Takafumi Hamachi, Katsumasa Maeda, Makoto Yokota, Ryuji Sakagami, Yoshitaka Hara, Kazuyuki Noguchi, Toshi Furuuchi, Takashi Sasano, Enyu Imai, Masatoshi Ohmae, Hayuru Koizumi, Mitsuru Watanuki, Shinya Murakami

    Journal of Bone and Mineral Research   31 ( 4 )   806 - 814   2016年4月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1002/jbmr.2738

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  • Involvement of an Skp-Like Protein, PGN_0300, in the Type IX Secretion System of Porphyromonas gingivalis 査読

    Yuko Taguchi, Keiko Sato, Hideharu Yukitake, Tetsuyoshi Inoue, Masaaki Nakayama, Mariko Naito, Yoshio Kondo, Konami Kano, Tomonori Hoshino, Koji Nakayama, Shogo Takashiba, Naoya Ohara

    Infection and Immunity   84 ( 1 )   230 - 240   2016年1月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    <title>ABSTRACT</title>

    The oral Gram-negative anaerobic bacterium
    <named-content content-type="genus-species">Porphyromonas gingivalis</named-content>
    is an important pathogen involved in chronic periodontitis. Among its virulence factors, the major extracellular proteinases, Arg-gingipain and Lys-gingipain, are of interest given their abilities to degrade host proteins and process other virulence factors. Gingipains possess C-terminal domains (CTDs) and are translocated to the cell surface or into the extracellular milieu by the type IX secretion system (T9SS). Gingipains contribute to the colonial pigmentation of the bacterium on blood agar. In this study, Omp17, the PGN_0300 gene product, was found in the outer membrane fraction. A mutant lacking Omp17 did not show pigmentation on blood agar and showed reduced proteolytic activity of the gingipains. CTD-containing proteins were released from bacterial cells without cleavage of the CTDs in the
    <italic>omp17</italic>
    mutant. Although synthesis of the anionic polysaccharide (A-LPS) was not affected in the
    <italic>omp17</italic>
    mutant, the processing of and A-LPS modification of CTD-containing proteins was defective. PorU, a C-terminal signal peptidase that cleaves the CTDs of other CTD-containing proteins, was not detected in any membrane fraction of the
    <italic>omp17</italic>
    mutant, suggesting that the defective maturation of CTD-containing proteins by impairment of Omp17 is partly due to loss of function of PorU. In the mouse subcutaneous infection experiment, the
    <italic>omp17</italic>
    mutant was less virulent than the wild type. These results suggested that Omp17 is involved in
    <named-content content-type="genus-species">P. gingivalis</named-content>
    virulence.

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  • Analysis of the relationship between periodontal disease and atherosclerosis within a local clinical system: a cross-sectional observational pilot study.

    Chieko Kudo, Wee Soo Shin, Masato Minabe, Kazuo Harai, Kai Kato, Hiroaki Seino, Eiji Goke, Nobuhiro Sasaki, Takemasa Fujino, Nobuichi Kuribayashi, Youko Onuki Pearce, Masato Taira, Hiroshi Maeda, Shogo Takashiba

    Odontology   103 ( 3 )   314 - 21   2015年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    It has been revealed that atherosclerosis and periodontal disease may have a common mechanism of "chronic inflammation". Several reports have indicated that periodontal infection is related to atherosclerosis, but none have yet reported such an investigation through the cooperation of local clinics. This study was performed in local Japanese clinics to examine the relationship between periodontal disease and atherosclerosis under collaborative medical and dental care. A pilot multicenter cross-sectional study was conducted on 37 medical patients with lifestyle-related diseases under consultation in participating medical clinics, and 79 periodontal patients not undergoing medical treatment but who were seen by participating dental clinics. Systemic examination and periodontal examination were performed at baseline, and the relationships between periodontal and atherosclerosis-related clinical markers were analyzed. There was a positive correlation between LDL-C level and plasma IgG antibody titer to Porphyromonas gingivalis. According to the analysis under adjusted age, at a cut-off value of 5.04 for plasma IgG titer to Porphyromonas gingivalis, the IgG titer was significantly correlated with the level of low-density lipoprotein cholesterol (LDL-C). This study suggested that infection with periodontal bacteria (Porphyromonas gingivalis) is associated with the progression of atherosclerosis. Plasma IgG titer to Porphyromonas gingivalis may be useful as the clinical risk marker for atherosclerosis related to periodontal disease. Moreover, the application of the blood examination as a medical check may lead to the development of collaborative medical and dental care within the local medical clinical system for the purpose of preventing the lifestyle-related disease.

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  • Identification of transactivation-responsive DNA-binding protein 43 (TARDBP43; TDP-43) as a novel factor for TNF-α expression upon lipopolysaccharide stimulation in human monocytes. 国際誌

    H Murata, T Hattori, H Maeda, S Takashiba, M Takigawa, J Kido, T Nagata

    Journal of periodontal research   50 ( 4 )   452 - 60   2015年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND AND OBJECTIVE: Tumor necrosis factor alpha (TNF-α) is a major cytokine implicated in various inflammatory diseases. The nature of the nuclear factors associated with human TNF-α gene regulation is not well elucidated. We previously identified a novel region located from -550 to -487 in human TNF-α promoter that did not contain the reported binding sites for nuclear factor kappa B (NF-κB) but showed lipopolysaccharide (LPS)-induced transcriptional activity. The purpose of this study is to identify novel factors that bind to the promoter region and regulate TNF-α expression. MATERIAL AND METHODS: To identify DNA-binding proteins that bound to the target region of TNF-α promoter, a cDNA library from LPS-stimulated human monocytic cell line THP-1 was screened using a yeast one-hybrid system. Cellular localizations of the DNA-binding protein in the cells were examined by subcellular immunocytochemistry. Nuclear amounts of the protein in LPS-stimulated THP-1 cells were identified by western blot analysis. Expression of mRNA of the protein in the cells was quantified by real-time polymerase chain reaction. Electrophoretic mobility shift assays were performed to confirm the DNA-binding profile. Overexpression of the protein and knockdown of the gene were also performed to investigate the role for TNF-α expression. RESULTS: Several candidates were identified from the cDNA library and transactivation-responsive DNA-binding protein 43 (TARDBP43; TDP-43) was focused on. Western blot analysis revealed that nuclear TDP-43 protein was increased in the LPS-stimulated THP-1 cells. Expression of TDP-43 mRNA was already enhanced before TNF-α induction by LPS. Electrophoretic mobility shift assay analysis showed that nuclear extracts obtained by overexpressing FLAG-tagged TDP-43 bound to the -550 to -487 TNF-α promoter fragments. Overexpression of TDP-43 in THP-1 cells resulted in an increase of TNF-α expression. Knockdown of TDP-43 in THP-1 cells downregulated TNF-α expression. CONCLUSION: We identified TDP-43 as one of the novel TNF-α factors and found that it bound to the LPS-responsive element in the TNF-α promoter to increase TNF-α expression.

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  • Cut-off values of Functional Independence Measure scores for discharge destination 査読

    Koji Naruishi, Akiko Kunita, Toshihiko Nagata, Shogo Takashiba, Seiji Adachi

    Geriatrics & Gerontology International   15 ( 5 )   670 - 671   2015年5月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

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  • With regard to our manuscripts on the commercial saliva substitute, Oralbalance®—its formula has been changed 査読

    Yuko Sugiura, Yoshihiko Soga, Ichiro Tanimoto, Susumu Kokeguchi, Sachiko Morishige-Nishide, Kotoe Itami-Kono, Kanayo Takahashi, Nobuharu Fujii, Fumihiko Ishimaru, Mitsune Tanimoto, Kokoro Yamabe, Soichiro Tsutani, Fusanori Nishimura, Shogo Takashiba

    Supportive Care in Cancer   22 ( 12 )   3121 - 3122   2014年12月

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    担当区分:最終著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

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  • Predictors of improved functional outcome in elderly inpatients after rehabilitation: a&nbsp;retrospective study 査読

    Koji Naruishi, Akiko Kunita, Katsuyuki Kubo, Toshihiko Nagata, Shogo Takashiba, Seiji Adachi

    Clinical Interventions in Aging   2133 - 2133   2014年12月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Informa UK Limited  

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  • Oral infection control to assist infliximab therapy in a Behçet's disease patient with severe eye inflammation in response to dental treatment: a case report

    Chieko Kudo, Hiroshi Wakabayashi, Masayuki Shimoe, Hiroya Kobayashi, Takashi Ito, Toshinori Ohkawa, Arisa Isoshima‐Nakamura, Junji Mineshiba, Norie Yoshioka, Kumiko Nawachi, Hiroshi Maeda, Toshihiko Matsuo, Hirofumi Makino, Shogo Takashiba

    Clinical Case Reports   2 ( 6 )   274 - 280   2014年12月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

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  • Synthetic (+)-terrein suppresses interleukin-6/soluble interleukin-6 receptor induced-secretion of vascular endothelial growth factor in human gingival fibroblasts 査読

    Hiroki Mandai, Kazuhiro Omori, Daisuke Yamamoto, Toki Tsumura, Kyouta Murota, Satoshi Yamamoto, Koichi Mitsudo, Soichiro Ibaragi, Akira Sasaki, Hiroshi Maeda, Shogo Takashiba, Seiji Suga

    Bioorganic & Medicinal Chemistry   22 ( 19 )   5338 - 5344   2014年10月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.bmc.2014.07.047

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  • Distribution of oral mucosal bacteria with mecA in patients undergoing hematopoietic cell transplantation 査読

    Takayuki Ebinuma, Yoshihiko Soga, Takamaro Sato, Kazuyuki Matsunaga, Chieko Kudo, Hiroshi Maeda, Yoshinobu Maeda, Mitsune Tanimoto, Shogo Takashiba

    Supportive Care in Cancer   22 ( 6 )   1679 - 1683   2014年6月

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    担当区分:最終著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

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  • Overexpression of Smad2 inhibits proliferation of gingival epithelial cells. 査読 国際誌

    M Shimoe, T Yamamoto, N Shiomi, K Tomikawa, S Hongo, K Yamashiro, T Yamaguchi, H Maeda, S Takashiba

    Journal of periodontal research   49 ( 3 )   290 - 8   2014年6月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND AND OBJECTIVE: Spatiotemporal inhibition of apical migration and proliferation of gingival epithelium are significant factors involved in periodontal regeneration. Transforming growth factor β (TGF-β) is important in multiple aspects of wound healing, and Smad2, a downstream transcription factor of TGF-β, has an inhibitory effect on re-epithelialization during gingival wound healing. Therefore, we investigated the effects on migration and proliferation status, and intra/extracellular signaling regulated by Smad2 overexpression in gingival epithelial cells. MATERIAL AND METHODS: Gingival epithelial cells were isolated from the palatal gingival tissue of transgenic mice overexpressing Smad2 driven by the Keratin14 promoter. Smad2 expression was identified by western blotting and immunofluorescence analysis. Scratch assay and 5-bromo-2'-deoxyuridine staining were performed to assess cell migration and proliferation. To inactivate TGF-β type I receptor, the cultures were supplemented with SB431542. Secreted TGF-β was quantified by ELISA. Smad2 target gene expression was examined by real-time RT-PCR and in vivo immunofluorescence analysis of gingival junctional epithelium. RESULTS: Smad2-overexpressing cells were confirmed to have significant phosphorylated Smad2 in the nucleus. Scratch assay and 5-bromo-2'-deoxyuridine staining indicated that Smad2-overexpressing cells showed no significant differences in migration, but had reduced proliferation rates compared to wild-type controls. SB431542 significantly inhibited Smad2 phosphorylation, which coincided with restoration of the proliferation rate in Smad2-overexpressing cells. ELISA of TGF-β release did not show any differences between genotypes. The cell cycle inhibitors, p15 and p21, showed significant upregulation in Smad2-overexpressing cells compared to wild-type controls. Moreover, junctional epithelium of the transgenic mice showed increased expression of P-Smad2, p15 and p21. CONCLUSION: The signaling activation triggered by overexpression of Smad2 was dependent on TGF-β type I receptor, and the activated Smad2 increased p15 and p21 expression, responsible for inhibiting cell cycle entry, resulting in antiproliferative effects on gingival epithelial cells. Understanding of Smad2-induced signaling would be useful for possible clinical application to regulate gingival epithelial downgrowth.

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  • Effect of Porphyromonas gingivalis outer membrane vesicles on gingipain-mediated detachment of cultured oral epithelial cells and immune responses 査読

    Ryoma Nakao, Shogo Takashiba, Saori Kosono, Minoru Yoshida, Haruo Watanabe, Makoto Ohnishi, Hidenobu Senpuku

    Microbes and Infection   16 ( 1 )   6 - 16   2014年1月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.micinf.2013.10.005

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  • Serum antibody response to group II chaperonin fromMethanobrevibacter oralisand human chaperonin CCT 査読

    Kimito Hirai, Hiroshi Maeda, Kazuhiro Omori, Tadashi Yamamoto, Susumu Kokeguchi, Shogo Takashiba

    Pathogens and Disease   68 ( 1 )   12 - 19   2013年6月

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    担当区分:最終著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

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  • Cytokine expression in human dermal fibroblasts stimulated with eosinophil cationic protein measured by protein array 査読

    Takamaro Sato, Yoshihiko Soga, Tomoko Yamaguchi, Michio Meguro, Hiroshi Maeda, Joji Tada, Takayuki Otani, Masaharu Seno, Shogo Takashiba

    Asian Pacific Journal of Allergy and Immunology   31 ( 4 )   2013年4月

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    担当区分:最終著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Allergy, Asthma, and Immunology Association of Thailand  

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  • Antibiotic sensitivity of bacteria on the oral mucosa after hematopoietic cell transplantation 査読

    Soga Yoshihiko, Maeda Yoshinobu, Tanimoto Mitsune, Ebinuma Takayuki, Maeda Hiroshi, Takashiba Shogo

    Supportive Care in Cancer   21 ( 2 )   367 - 368   2013年2月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer-Verlag  

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  • Assessment of the Plasma/Serum IgG Test to Screen for Periodontitis 査読

    C. Kudo, K. Naruishi, H. Maeda, Y. Abiko, T. Hino, M. Iwata, C. Mitsuhashi, S. Murakami, T. Nagasawa, T. Nagata, S. Yoneda, Y. Nomura, T. Noguchi, Y. Numabe, Y. Ogata, T. Sato, H. Shimauchi, K. Yamazaki, A. Yoshimura, S. Takashiba

    Journal of Dental Research   91 ( 12 )   1190 - 1195   2012年12月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE Publications  

    Chronic periodontitis is a silent infectious disease prevalent worldwide and affects lifestyle-related diseases. Therefore, efficient screening of patients is essential for general health. This study was performed to evaluate prospectively the diagnostic utility of a blood IgG antibody titer test against periodontal pathogens. Oral examination was performed, and IgG titers against periodontal pathogens were measured by ELISA in 1,387 individuals. The cut-off value of the IgG titer was determined in receiver operating characteristic curve analysis, and changes in periodontal clinical parameters and IgG titers by periodontal treatment were evaluated. The relationships between IgG titers and severity of periodontitis were analyzed. The best cut-off value of IgG titer against Porphyromonas gingivalis for screening periodontitis was 1.682. Both clinical parameters and IgG titers decreased significantly under periodontal treatment. IgG titers of periodontitis patients were significantly higher than those of healthy controls, especially in those with sites of probing pocket depth over 4 mm. Multiplied cut-off values were useful to select patients with severe periodontitis. A blood IgG antibody titer test for Porphyromonas gingivalis is useful to screen hitherto chronic periodontitis patients (ClinicalTrials.gov number NCT01658475).

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  • Smad2 Decelerates Re-epithelialization during Gingival Wound Healing 査読

    K. Tomikawa, T. Yamamoto, N. Shiomi, M. Shimoe, S. Hongo, K. Yamashiro, T. Yamaguchi, H. Maeda, S. Takashiba

    Journal of Dental Research   91 ( 8 )   764 - 770   2012年8月

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    担当区分:最終著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE Publications  

    During periodontal regeneration, inhibition of gingival downgrowth is necessary to promote migration of mesenchymal cells into the defects. Transforming growth factor (TGF)-β is a pleiotropic cytokine that has numerous cell functions, including regulation of epithelial growth. Recent studies have shown that Smad2, a downstream transcription factor of TGF-β, plays crucial roles in wound healing in the epithelia. Therefore, we investigated the effects of Smad2 overexpression on re-epithelialization of gingival wounds. Transgenic mice overexpressing smad2 driven by the keratin 14 promoter ( k14-smad2) were confirmed to have significant Smad2 phosphorylation in gingival basal epithelia. Punch wounds were made in the palatal gingiva, and wound healing was assessed histologically for 7 days. Re-epithelialization was significantly retarded on day 2, while collagen deposition was enhanced on day 7 in k14-smad2 compared with wild-type mice. Moreover, expression of keratin 16 (K16), an indicator of keratinocyte migration, was significantly inhibited in wound-edge keratinocytes in k14-smad2. The inhibition of K16 coincided with the induction of Smad2 in the corresponding epithelia, while BrdU incorporation was unaffected. These results indicated that Smad2 has inhibitory effects in regulating keratinocyte migration during gingival wound healing. TGF-β/Smad2 signaling mediating alteration of K16 expression must be tightly regulated during periodontal regeneration.

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  • Relationship between Periodontitis-Related Antibody and Frequent Exacerbations in Chronic Obstructive Pulmonary Disease 査読

    Tamaki Takahashi, Shigeo Muro, Naoya Tanabe, Kunihiko Terada, Hirofumi Kiyokawa, Susumu Sato, Yuma Hoshino, Emiko Ogawa, Kazuko Uno, Koji Naruishi, Shogo Takashiba, Michiaki Mishima

    PLoS ONE   7 ( 7 )   e40570 - e40570   2012年7月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Public Library of Science (PLoS)  

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  • Histological and immunohistochemical features of gingival enlargement in a patient with AML 査読

    Norihiro Sonoi, Yoshihiko Soga, Hiroshi Maeda, Koichi Ichimura, Tadashi Yoshino, Kazutoshi Aoyama, Nobuharu Fujii, Yoshinobu Maeda, Mitsune Tanimoto, Richard Logan, Judith Raber-Durlacher, Shogo Takashiba

    Odontology   100 ( 2 )   254 - 257   2012年7月

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    担当区分:最終著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    DOI: 10.1007/s10266-011-0051-0

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  • 特定非営利活動法人としての研究を考える

    高柴 正悟

    日本歯周病学会会誌   54 ( 1 )   3 - 4   2012年3月

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    記述言語:日本語   出版者・発行元:JAPANESE SOCIETY OF PERIODONTOLOGY  

    DOI: 10.2329/perio.54.3

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  • Influence of resin coating materials on Porphyromonas gingivalis attachment 査読

    Ai KUMADA, Yoshizo MATSUKA, Atsushi MINE, Mitsuaki ONO, Junji UEHARA, Norihiro SONOI, Takashi ITO, Shogo TAKASHIBA, Takuo KUBOKI

    Dental Materials Journal   31 ( 1 )   86 - 91   2012年

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Japanese Society for Dental Materials and Devices  

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  • Outer Membrane Vesicles of Porphyromonas gingivalis Elicit a Mucosal Immune Response 査読

    Ryoma Nakao, Hideki Hasegawa, Kuniyasu Ochiai, Shogo Takashiba, Akira Ainai, Makoto Ohnishi, Haruo Watanabe, Hidenobu Senpuku

    PLoS ONE   6 ( 10 )   e26163 - e26163   2011年10月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Public Library of Science (PLoS)  

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  • Bacterial substitution of coagulase-negative staphylococci for streptococci on the oral mucosa after hematopoietic cell transplantation 査読

    Soga Yoshihiko, Maeda Yoshinobu, Ishimaru Fumihiko, Tanimoto Mitsune, Maeda Hiroshi, Nishimura Fusanori, Takashiba Shogo

    Supportive Care in Cancer   19 ( 7 )   995 - 1000   2011年7月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Coagulase-negative staphylococci (CoNS) are frequently isolated from blood cultures of hematopoietic cell transplantation (HCT) patients. Generally, the use of central venous catheters is recognized as a significant risk factor for CoNS infection, while the impact of CoNS infection from oral ulcerative mucositis, which occurs frequently in HCT, may be underestimated. Here, we examined the bacteria on the buccal mucosa after HCT. Sixty-one patients were examined for bacteria on the buccal mucosa routinely once a week from 1 week before to 3 weeks after allogeneic HCT. Subjects were divided into groups with short and long periods of antibiotic use, and differences in bacterial substitution were evaluated. The relationships between type of HCT (conventional HCT or RIST) and bacterial substitution were also evaluated. The changes in detection frequencies of CoNS and alpha-streptococci from before to 3 weeks after HCT were significant (P < 0.05, chi (2) test): 14.5-53.3% and 92.7-53.1%, respectively. Significant bacterial substitution of CoNS for streptococci was observed in the long-term antibiotic use group (P < 0.05, chi (2) test), but also occurred in cases with short-term or no antibiotic use. No relationships between type of HCT (conventional HCT or RIST) were observed. Bacterial substitution of CoNS for streptococci occurred frequently on the buccal mucosa after HCT. In addition to antibiotic use, environmental factors may be involved in bacterial substitution. It is important to consider the presence of oral mucositis in CoNS infection after HCT.

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  • Chronic periodontitis with multiple risk factor syndrome: a case report. 査読 国際誌

    Shimoe M, Yamamoto T, Iwamoto Y, Shiomi N, Maeda H, Nishimura F, Takashiba S

    Journal of the International Academy of Periodontology   13 ( 2 )   40 - 47   2011年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:2  

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  • Specific In Situ Visualization of Plasma Cells Producing Antibodies against Porphyromonas gingivalis in Gingival Radicular Cyst 査読

    Shinya Tsuge, Yasuyoshi Mizutani, Kazuhiro Matsuoka, Tatsuya Sawasaki, Yaeta Endo, Koji Naruishi, Hiroshi Maeda, Shogo Takashiba, Kazuya Shiogama, Ken-ichi Inada, Yutaka Tsutsumi

    Journal of Histochemistry & Cytochemistry   59 ( 7 )   673 - 689   2011年7月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE Publications  

    The enzyme-labeled antigen method was applied to visualize plasma cells producing antibodies to Porphyromonas gingivalis, flora of the human oral cavity. Antibodies to P. gingivalis have reportedly been detected in sera of patients with periodontitis. Biotinylated bacterial antigens, Ag53, and four gingipain domains (Arg-pro, Arg-hgp, Lys-pro, and Lys-hgp) were prepared by the cell-free protein synthesis system using the wheat germ extract. In paraformaldehyde-fixed frozen sections of rat lymph nodes experimentally immunized with Ag53-positive and Ag53-negative P. gingivalis, plasma cells were labeled with biotinylated Arg-hgp and Lys-hgp. Antibodies to Ag53 were detected only in the nodes immunized with Ag53-positive bacteria. In two of eight lesions of gingival radicular cyst with inflammatory infiltration, CD138-positive plasma cells in frozen sections were signalized for Arg-hgp and Lys-hgp. An absorption study using unlabeled antigens confirmed the specificity of staining. The AlphaScreen method identified the same-type antibodies in tissue extracts but not in sera. Antibodies to Ag53, Arg-pro, and Lys-pro were undetectable. In two cases, serum antibodies to Arg-hgp and Lys-hgp were AlphaScreen positive, whereas plasma cells were scarcely observed within the lesions. These findings indicate the validity of the enzyme-labeled antigen method. This is the very first application of this novel histochemical technique to human clinical samples.

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  • Discovery of a patient with strongly suspected bullous pemphigoid in a ward by oral health care providers 査読

    N Kanda, Y Soga, M Meguro, A Tanabe, Y Yagi, Y Himuro, Y Fujiwara, S Takashiba, N Kobayashi

    International Journal of Dental Hygiene   9 ( 2 )   159 - 162   2011年5月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

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  • IMMUNE RESPONSES TO PORPHYROMONAS GINGIVALIS INFECTION SUPPRESS SYSTEMIC INFLAMMATORY RESPONSE IN EXPERIMENTAL MURINE MODEL 査読

    K. Naruishi, K. Omori, H. Maeda, N. Sonoi, K. Funakoshi, K. Hirai, M. Ishii, K. Kubo, H. Kobayashi, T. Tomiyama, D. Yamamoto, I. Tanimoto, K. Kunimatsu, S. Takashiba

    JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS   25 ( 2 )   195 - 202   2011年4月

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    担当区分:最終著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BIOLIFE SAS  

    Periodontitis is localized infectious disease caused by periodontopathic bacteria such as Porphyromonas gingivalis (T: gingivalis), and the severity correlates to significance of immune responses. Recently, it has been reported that periodontitis is associated with the development of systemic disease such as diabetes and atherosclerosis because of increasing invasion of oral pathogens to the circulation. However, the association between local and systemic infectious responses is still unclear. In the present study, we examined the differences of biological responses in animals with or without bacterial infection. After Balb/c mice were infected subcutaneously with live P gingivalis W83, serum, skin and liver were collected according to experimental protocol. The skin and liver tissues were observed pathologically by haematoxylin-eosin staining, and serum IL-6 levels were measured using ELISA method. Throughout the experimental period, conditions of the mice were observed continuously. As expected, severe infiltration of leukocytes were observed at inflamed skin corresponding to the number of bacterial challenges. Although no inflammatory appearance of skin was observed, serum IL-6 levels were increased dramatically (P &lt; 0.01, Student&apos;s t-test) and liver tissues were injured in the mice without bacterial challenge. Interestingly, although severe inflammatory appearance of the skin was observed, serum IL-6 levels were not increased and no inflammatory responses were observed in the liver of the 3-times bacterially challenged group. Importantly, immunoglobulin G against P gingivalis W83 was detected in the blood of mice with 3-times bacterial challenge corresponding to improvement of weight loss and survival. In conclusion, although multiple infections develop severe localized inflammation, the immune system should be sufficient to protect the systemic inflammatory responses.

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  • Progress of oral care and reduction of oral mucositis-a pilot study in a hematopoietic stem cell transplantation ward 査読

    Soga Yoshihiko, Sugiura Yuko, Takahashi Kanayo, Nishimoto Hitomi, Maeda Yoshinobu, Tanimoto Mitsune, Takashiba Shogo

    Supportive Care in Cancer   19 ( 2 )   303 - 307   2011年2月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Oral mucositis is a common symptomatic complication associated with hematopoietic stem cell transplantation (HCT). We use simple strategies aimed to reduce oral mucositis by keeping the oral cavity clean and moist. Here, we report on the progress of oral care and the changes in the degree of oral mucositis. The purpose of this pilot study is to evaluate the effects of our strategies on the prevalence and the severity of oral mucositis. Fifty-three consecutive patients from 2003 to 2006 administered with conventional allogeneic HCT were enrolled in this study. The degree of oral mucositis was evaluated daily in all patients. Our oral care program was divided into two periods: "examination and trial period (2003 and 2004)" and "intensive oral care period (2005 and 2006)." In the latter, an oral care regimen was carried out systematically by a multidisciplinary team. Using our oral care strategies, the prevalence of ulcerative oral mucositis was decreased significantly. The rate was reduced from 76% (10 of 13) of patients with ulcerative oral mucositis in 2003 to only 20% (3 of 15) in 2006. Our pilot study suggests that oral mucositis in HCT patients can be alleviated by simple strategies aimed at keeping the oral cavity clean and moist.

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  • FGF-2 Stimulates Periodontal Regeneration: results of a multi-center randomized clinical trial 査読

    M. Kitamura, M. Akamatsu, M. Machigashira, Y. Hara, R. Sakagami, T. Hirofuji, T. Hamachi, K. Maeda, M. Yokota, J. Kido, T. Nagata, H. Kurihara, S. Takashiba, T. Sibutani, M. Fukuda, T. Noguchi, K. Yamazaki, H. Yoshie, K. Ioroi, T. Arai, T. Nakagawa, K. Ito, S. Oda, Y. Izumi, Y. Ogata, S. Yamada, H. Shimauchi, K. Kunimatsu, M. Kawanami, T. Fujii, Y. Furuichi, T. Furuuchi, T. Sasano, E. Imai, M. Omae, S. Yamada, M. Watanuki, S. Murakami

    Journal of Dental Research   90 ( 1 )   35 - 40   2011年1月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE Publications  

    The efficacy of the local application of recombinant human fibroblast growth factor-2 (FGF-2) in periodontal regeneration has been investigated. In this study, a randomized, double-blind, placebo-controlled clinical trial was conducted in 253 adult patients with periodontitis. Modified Widman periodontal surgery was performed, during which 200 µL of the investigational formulation containing 0% (vehicle alone), 0.2%, 0.3%, or 0.4% FGF-2 was administered to 2- or 3-walled vertical bone defects. Each dose of FGF-2 showed significant superiority over vehicle alone (p &lt; 0.01) for the percentage of bone fill at 36 wks after administration, and the percentage peaked in the 0.3% FGF-2 group. No significant differences among groups were observed in clinical attachment regained, scoring approximately 2 mm. No clinical safety problems, including an abnormal increase in alveolar bone or ankylosis, were identified. These results strongly suggest that topical application of FGF-2 can be efficacious in the regeneration of human periodontal tissue that has been destroyed by periodontitis.

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  • Prognosis of periodontitis recurrence after intensive periodontal treatment using examination of serum IgG antibody titer against periodontal bacteria 査読

    Noriko Sugi, Koji Naruishi, Chieko Kudo, Aya Hisaeda-Kako, Takayuki Kono, Hiroshi Maeda, Shogo Takashiba

    Journal of Clinical Laboratory Analysis   25 ( 1 )   25 - 32   2011年

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    担当区分:最終著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1002/jcla.20381

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  • IL-6/sIL-6RはRAW264.7細胞におけるRANKL誘導性の破骨細胞分化を抑制し,IL-8の産生を亢進する

    久保 克行, 大森 一弘, 成石 浩司, 冨山 高史, 小林 寛也, 山本 大介, 前田 博史, 高柴 正悟

    特定非営利活動法人 日本歯周病学会学術大会 プログラムおよび講演抄録集   2011   19 - 19   2011年

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    記述言語:日本語   出版者・発行元:特定非営利活動法人 日本歯周病学会  

    DOI: 10.14833/amjsp.2011s.0.19.0

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  • Smad2による歯肉創傷治癒の再上皮化抑制

    冨川 和哉, 山本 直史, 下江 正幸, 塩見 信行, 峯柴 淳二, 山口 知子, 本郷 昌一, 前田 博史, 高柴 正悟

    特定非営利活動法人 日本歯周病学会学術大会 プログラムおよび講演抄録集   2011   21 - 21   2011年

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    記述言語:日本語   出版者・発行元:特定非営利活動法人 日本歯周病学会  

    DOI: 10.14833/amjsp.2011s.0.21.0

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  • 血中IgG抗体価測定に用いるPorphyromonas gingivalis抗原タンパク質の選抜と合成

    野添 幹雄, 山口 知子, 工藤 値英子, 山部 こころ, 平井 公人, 江口 徹, 前田 博史, 高柴 正悟

    特定非営利活動法人 日本歯周病学会学術大会 プログラムおよび講演抄録集   2011   5 - 5   2011年

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    記述言語:日本語   出版者・発行元:特定非営利活動法人 日本歯周病学会  

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  • 歯周病治療後の予後管理 SPT期のセルフケアの工夫

    高柴 正悟

    特定非営利活動法人 日本歯周病学会学術大会 プログラムおよび講演抄録集   2011   73 - 73   2011年

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    記述言語:日本語   出版者・発行元:特定非営利活動法人 日本歯周病学会  

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  • 血中IgG抗体価検査用のPorphyromonas gingivalis合成抗原タンパク質の患者血清との反応性

    山口 知子, 野添 幹雄, 工藤 値英子, 山部 こころ, 平井 公人, 江口 徹, 前田 博史, 高柴 正悟

    特定非営利活動法人 日本歯周病学会学術大会 プログラムおよび講演抄録集   2011   6 - 6   2011年

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    記述言語:日本語   出版者・発行元:特定非営利活動法人 日本歯周病学会  

    DOI: 10.14833/amjsp.2011s.0.6.0

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  • Clinical and Immunological Assessment of Periodontal Disease in Japanese Leprosy Patients 査読

    Hideki Ohyama, Hiroshi Hongyo, Naoko Shimizu, Yoshikazu Shimizu, Fusanori Nishimura, Masatsugu Nakagawa, Hideo Arai, Nahoko Kato-Kogoe, Nobuyuki Terada, Atsushi Nagai, Shogo Takashiba, Hidemi Kurihara, Yoshio Nomura, Yoji Murayama

    JAPANESE JOURNAL OF INFECTIOUS DISEASES   63 ( 6 )   427 - 432   2010年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATL INST INFECTIOUS DISEASES  

    Periodontitis is a chronic inflammatory disease caused by the infection of periodontopathic bacteria in dental plaque However, an individual's susceptibility to this disease appears to be associated with multiple genetic factors, as seen in the case of leprosy In order to gain a better understanding of the pathophysiology of periodontal disease in subjects with leprosy, we investigated the clinical features of periodontitis and the immunological responses against periodontopathic bacteria in 382 subjects with a history of leprosy and 451 age-matched control subjects The prevalence of periodontitis and the degree of periodontal pocket depth were found to be significantly higher in leprosy patients than in age-matched controls Furthermore, a comparison of the clinical parameters of lepromatous leprosy (L-lep) and tuberculoid leprosy (T-lep) patients showed that the probing pocket depth of L-lep patients with periodontal disease was significantly higher than that for T-lep patients In contrast, serum IgG titers against Porphyromonas gingivalis in L-lep patients were significantly lower than in T-lep patients These results imply that L-lep patients show more severe periodontal disease than T-lep patients or age-matched control subjects, and that low humoral immunity against P gingivalis might be one of the genetic factors determining periodontal disease susceptibility in leprosy patients

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  • The inhibition of DNA synthesis by prostaglandin E2 in human gingival fibroblasts is independent of the cyclic AMP-protein kinase A signal transduction pathway 査読

    H. Arai, Y. Nomura, M. Kinoshita, F. Nishimura, M. Takigawa, K. Takahashi, N. Washio, S. Takashiba, Y. Murayama

    Journal of Periodontal Research   33 ( 1 )   33 - 39   2010年6月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/j.1600-0765.1998.tb02289.x

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  • Antigenic group II chaperonin inMethanobrevibacter oralismay cross-react with human chaperonin CCT 査読

    K. Yamabe, H. Maeda, S. Kokeguchi, Y. Soga, M. Meguro, K. Naruishi, S. Asakawa, S. Takashiba

    Molecular Oral Microbiology   25 ( 2 )   112 - 122   2010年4月

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    担当区分:最終著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/j.2041-1014.2009.00548.x

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  • Rapid detection ofmecAandspaby the loop-mediated isothermal amplification (LAMP) method 査読 国際誌

    Y. Koide, H. Maeda, K. Yamabe, K. Naruishi, T. Yamamoto, S. Kokeguchi, S. Takashiba

    Letters in Applied Microbiology   50 ( 4 )   386 - 392   2010年4月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    Aim:
    To develop a detection assay for staphylococcal mecA and spa by using loop-mediated isothermal amplification (LAMP) method.
    Methods and Results:
    Staphylococcus aureus and other related species were subjected to the detection of mecA and spa by both PCR and LAMP methods. The LAMP successfully amplified the genes under isothermal conditions at 64 degrees C within 60 min, and demonstrated identical results with the conventional PCR methods. The detection limits of the LAMP for mecA and spa, by gel electrophoresis, were 102 and 10 cells per tube, respectively. The naked-eye inspections were possible with 103 and 10 cells for detection of mecA and spa, respectively. The LAMP method was then applied to sputum and dental plaque samples. The LAMP and PCR demonstrated identical results for the plaque samples, although frequency in detection of mecA and spa by the LAMP was relatively lower for the sputum samples when compared to the PCR methods.
    Conclusion:
    Application of the LAMP enabled a rapid detection assay for mecA and spa. The assay may be applicable to clinical plaque samples.
    Significance and Impact of the Study:
    The LAMP offers an alternative detection assay for mecA and spa with a great advantage of the rapidity.

    DOI: 10.1111/j.1472-765x.2010.02806.x

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  • Highly expressed genes in a rough-colony-forming phenotype ofAggregatibacter actinomycetemcomitans: implication of amip-like gene for the invasion of host tissue 査読

    Takemasa Maeda, Hiroshi Maeda, Kokoro Yamabe, Junji Mineshiba, Ichiro Tanimoto, Tadashi Yamamoto, Koji Naruishi, Susumu Kokeguchi, Shogo Takashiba

    FEMS Immunology & Medical Microbiology   58 ( 2 )   226 - 236   2010年3月

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    担当区分:最終著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

    DOI: 10.1111/j.1574-695x.2009.00624.x

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  • Total bacterial counts on oral mucosa after using a commercial saliva substitute in patients undergoing hematopoietic cell transplantation 査読

    Sugiura Yuko, Soga Yoshihiko, Yamabe Kokoro, Tsutani Soichiro, Tanimoto Ichiro, Maeda Hiroshi, Kokeguchi Susumu, Fujii Nobuharu, Ishimaru Fumihiko, Tanimoto Mitsune, Nishimura Fusanori, Takashiba Shogo

    Supportive Care in Cancer   18 ( 3 )   395 - 398   2010年3月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The commercial saliva substitute OralbalanceA (R) has been reported to alleviate symptoms of postradiotherapy xerostomia in head and neck cancer patients. OralbalanceA (R) may also be effective for xerostomia in patients undergoing hematopoietic cell transplantation (HCT) with high-dose chemotherapy and total-body irradiation. However, HCT patients are in a severely compromised condition, and saliva substitute must not promote infection. We reported previously that OralbalanceA (R) has antimicrobial effects against microbial species detected during HCT in vitro. This study was performed to determine the in vivo effects of OralbalanceA (R) on oral mucosal total bacterial counts in patients undergoing HCT. A total of 18 neutropenic patients undergoing HCT were enrolled in this study. Before and after 1 week of OralbalanceA (R) use, bacterial samples were obtained from patients by wiping an area of I center dot 1 cm on the buccal mucosa with sterilized cotton swabs. Total bacterial counts of the obtained samples were examined by quantitative polymerase chain reaction amplification of the bacterial 16S ribosomal RNA gene. As controls, bacterial samples were also obtained from ten healthy subjects, and total bacterial counts were examined. No significant increase in bacterial count was observed with use of OralbalanceA (R). None of the patients showed bacterial counts above the range found in healthy controls after using OralbalanceA (R). In neutropenic patients undergoing HCT, OralbalanceA (R) did not increase the total counts of oral mucosal bacteria beyond the range found in healthy controls. Oral care using OralbalanceA (R) may alleviate the symptoms induced by hyposalivation without promoting infection.

    DOI: 10.1007/s00520-009-0789-x

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  • Oral mucositis in patients receiving reduced-intensity regimens for allogeneic hematopoietic cell transplantation: comparison with conventional regimen 査読

    Kanayo Takahashi, Yoshihiko Soga, Yumeno Murayama, Mika Udagawa, Hitomi Nishimoto, Yuko Sugiura, Yoshinobu Maeda, Mitsune Tanimoto, Shogo Takashiba

    Supportive Care in Cancer   18 ( 1 )   115 - 119   2010年1月

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    担当区分:最終著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    DOI: 10.1007/s00520-009-0637-z

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  • Genetic Risk Factors for Periodontitis in a Japanese Population 査読

    KOBAYASHI T., NAGATA T., MURAKAMI S., TAKASHIBA S., KURIHARA H., IZUMI Y., NUMABE Y., WATANABE H., KATAOKA M., NAGAI A., HAYASHI J., OHYAMA H., OKAMATSU Y., INAGAKI Y., TAI H., YOSHIE H.

    Journal of dental research   88 ( 12 )   1137 - 1141   2009年12月

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  • Porphyromonas gingivalisfimbriae-dependent interleukin-6 autocrine regulation by increase of gp130 in endothelial cells 査読

    Y-S. Ho, M-T. Lai, S-J. Liu, C-T. Lin, K. Naruishi, S. Takashiba, H-H. Chou

    Journal of Periodontal Research   44 ( 4 )   550 - 556   2009年8月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/j.1600-0765.2008.01150.x

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  • Febrile neutropenia and periodontitis: lessons from a case periodontal treatment in the intervals between chemotherapy cycles for leukemia reduced febrile neutropenia 査読

    Soga Yoshihiko, Yamasuji Yoshiko, Kudo Chieko, Matsuura-Yoshimoto Kaori, Yamabe Kokoro, Sugiura Yuko, Maeda Yoshinobu, Ishimaru Fumihiko, Tanimoto Mitsune, Nishimura Fusanori, Takashiba Shogo

    Supportive Care in Cancer   17 ( 5 )   581 - 587   2009年5月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Oral and systemic infections arising from the oral cavity are significant problems in clinical management of patients undergoing leukemia treatment. However, there is significant disparity in the reported incidences of development of periodontal infections. Evidence is limited to those showing the systemic influence of periodontal infection in neutropenic patients. This study indicated an association between febrile neutropenia (FN) and periodontitis in a case in which periodontal treatment in the intervals between chemotherapy cycles reduced FN in subsequent courses of chemotherapy and hematopoietic transplantation (HCT). Periodontal treatment was performed in a 61-year-old man with advanced periodontitis, who received HCT following three cycles of chemotherapy. After recovery from neutropenia induced by initial chemotherapy, periodontal treatment was performed in each chemotherapy interval period. Following extraction of teeth with severe advanced periodontitis, all teeth were subjected to periodontal pocket curettage and root planning, which are common periodontal treatments to reduce periodontal pockets harboring anaerobic periodontal bacteria, before HCT. Periodontal treatment successfully reduced periodontal pockets from 4.1 +/- 1.5 mm to 3.0 +/- 0.6 mm, which was almost within the healthy range (< 3.0 mm), before HCT. The frequency of FN decreased significantly with increasing cycles of chemotherapy, and decreases in FN corresponded to progress of periodontal treatment. Blood cultures obtained a total of 12 times throughout leukemia treatment were all negative. The observations reported here indicate the importance of periodontal treatment in clinical management of patients undergoing leukemia treatment to prevent FN, although all blood cultures were negative.

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  • Antibacterial effect of bactericide immobilized in resin matrix 査読

    Naoko Namba, Yasuhiro Yoshida, Noriyuki Nagaoka, Seisuke Takashima, Kaori Matsuura-Yoshimoto, Hiroshi Maeda, Bart Van Meerbeek, Kazuomi Suzuki, Shogo Takashiba

    Dental Materials   25 ( 4 )   424 - 430   2009年4月

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    担当区分:最終著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.dental.2008.08.012

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  • Assessment of Chromosome 19 for Genetic Association in Severe Chronic Periodontitis 査読

    Koichi Tabeta, Yasuko Shimada, Hideaki Tai, Yuichi Ishihara, Toshihide Noguchi, Yoshihiko Soga, Shogo Takashiba, Genki Suzuki, Terukazu Kobayashi, Akira Oka, Tetsuo Kobayashi, Kazuhisa Yamazaki, Hidetoshi Inoko, Hiromasa Yoshie

    Journal of Periodontology   80 ( 4 )   663 - 671   2009年4月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1902/jop.2009.080516

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  • Human leukocyte histocompatibility antigen class II-induced cytokines from human gingival fibroblasts promote proliferation of human umbilical vein endothelial cells: potential association with enhanced angiogenesis in chronic periodontal inflammation 査読

    Y. Okada, M. Meguro, H. Ohyama, S. Yoshizawa, K. Takeuchi-Hatanaka, N. Kato, S. Matsushita, S. Takashiba, F. Nishimura

    Journal of Periodontal Research   44 ( 1 )   103 - 109   2009年2月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/j.1600-0765.2008.01097.x

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  • ショットガンクローニング法によるPorphyromonas gingivalisからのsmall non-coding RNAの同定

    山部 こころ, 前田 博史, 樋口 彩子, 園井 教裕, 目黒 道生, 谷本 一郎, 山本 直史, 成石 浩司, 苔口 進, 高柴 正悟

    特定非営利活動法人 日本歯周病学会学術大会 プログラムおよび講演抄録集   2009   24 - 24   2009年

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    記述言語:日本語   出版者・発行元:特定非営利活動法人 日本歯周病学会  

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  • 開咬と下顎前歯部の叢生によって増悪した中等度慢性歯周炎の一症例

    小出 康史, 成石 浩司, 河田 有祐, 岩本 義博, 峯柴 淳二, 前田 博史, 高柴 正悟

    特定非営利活動法人 日本歯周病学会学術大会 プログラムおよび講演抄録集   2009   124 - 124   2009年

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    記述言語:日本語   出版者・発行元:特定非営利活動法人 日本歯周病学会  

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  • IL-6/sIL-6R enhances cathepsin B and L production via caveolin-1-mediated JNK-AP-1 pathway in human gingival fibroblasts 査読

    Tomoko Yamaguchi, Koji Naruishi, Hideo Arai, Fusanori Nishimura, Shogo Takashiba

    Journal of Cellular Physiology   217 ( 2 )   423 - 432   2008年11月

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    担当区分:最終著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1002/jcp.21517

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  • Evaluation of xerostomia in hematopoietic cell transplantation by a simple capacitance method device 査読

    Sugiura Yuko, Soga Yoshihiko, Nishide Sachiko, Kono Kotoe, Takahashi Kanayo, Fujii Nobuharu, Ishimaru Fumihiko, Tanimoto Mitsune, Nishimura Fusanori, Takashiba Shogo

    Supportive Care in Cancer   16 ( 10 )   1197 - 1200   2008年10月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Goals Hematopoietic cell transplantation (HCT) may lead to the development of xerostomia. However, there have been few reports of xerostomia in HCT patients based on objective data. We investigated moisture in the oral mucosa in patients undergoing HCT by the capacitance method using a convenient device, Moisture Checker for Mucus (R) (MCM; Life Co., Ltd., Saitama, Japan). Subjects and methods Thirty-six patients undergoing HCT at Okayama University Hospital of Medicine and Dentistry (Male=22, Female=14; age=41.6 +/- 16.2 years old) were enrolled in this study. Moisture in the oral mucosa was measured by MCM in accordance with the manufacturer's instructions. The results were obtained as MCM values (%), which are the weight percentage of water content in the oral mucosal epithelium. As controls, moisture of the oral mucosa was also examined in healthy volunteers (Male=27, Female=35; age=43.0 +/- 14.6 years old). Main results Throughout the examination period, MCM values were significantly lower in patients who underwent HCT than in controls. The degree of mucosal moisture in HCT patients showed wide interindividual differences. Conclusion The degree of mucosal moisture in HCT patients was low and showed wide interindividual differences. Evaluation of xerostomia using such a device may contribute to appropriate oral care with saliva substitute.

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  • Distribution ofArchaeain Japanese patients with periodontitis and humoral immune response to the components 査読

    Kokoro Yamabe, Hiroshi Maeda, Susumu Kokeguchi, Ichiro Tanimoto, Norihiro Sonoi, Susumu Asakawa, Shogo Takashiba

    FEMS Microbiology Letters   287 ( 1 )   69 - 75   2008年10月

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    担当区分:最終著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

    DOI: 10.1111/j.1574-6968.2008.01304.x

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  • Rapid and simple detection of eight major periodontal pathogens by the loop-mediated isothermal amplification method 査読

    Junko Miyagawa, Hiroshi Maeda, Toshimitsu Murauchi, Susumu Kokeguchi, Kokoro Yamabe, Ichiro Tanimoto, Fusanori Nishimura, Kazuhiro Fukui, Shogo Takashiba

    FEMS Immunology & Medical Microbiology   53 ( 3 )   314 - 321   2008年8月

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    担当区分:最終著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

    DOI: 10.1111/j.1574-695x.2008.00417.x

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  • Periodontal Tissue Regeneration Using Fibroblast Growth Factor -2: Randomized Controlled Phase II Clinical Trial 査読

    Masahiro Kitamura, Keisuke Nakashima, Yusuke Kowashi, Takeo Fujii, Hidetoshi Shimauchi, Takashi Sasano, Toshi Furuuchi, Mitsuo Fukuda, Toshihide Noguchi, Toshiaki Shibutani, Yukio Iwayama, Shogo Takashiba, Hidemi Kurihara, Masami Ninomiya, Jun-ichi Kido, Toshihiko Nagata, Takafumi Hamachi, Katsumasa Maeda, Yoshitaka Hara, Yuichi Izumi, Takao Hirofuji, Enyu Imai, Masatoshi Omae, Mitsuru Watanuki, Shinya Murakami

    PLoS ONE   3 ( 7 )   e2611 - e2611   2008年7月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Public Library of Science (PLoS)  

    DOI: 10.1371/journal.pone.0002611

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  • Chemotactic response of periodontal ligament cells decreases with donor age: association with reduced expression of c-fos 査読

    Y. Asahara, F. Nishimura, H. Arai, H. Kurihara, S. Takashiba, Y. Murayama

    Oral Diseases   5 ( 4 )   337 - 343   2008年6月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/j.1601-0825.1999.tb00100.x

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  • Antimicrobial effects of the saliva substitute, Oralbalance®, against microorganisms from oral mucosa in the hematopoietic cell transplantation period 査読

    Yuko Sugiura, Yoshihiko Soga, Ichiro Tanimoto, Susumu Kokeguchi, Sachiko Nishide, Kotoe Kono, Kanayo Takahashi, Nobuharu Fujii, Fumihiko Ishimaru, Mitsune Tanimoto, Kokoro Yamabe, Soichiro Tsutani, Fusanori Nishimura, Shogo Takashiba

    Supportive Care in Cancer   16 ( 4 )   421 - 424   2008年4月

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    担当区分:最終著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    DOI: 10.1007/s00520-007-0391-z

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  • Polymorphisms in the 5' flanking region of IL12RB2 are associated with susceptibility to periodontal diseases in the Japanese population 査読

    Kazu Takeuchi-Hatanaka, Hideki Ohyama, Fusanori Nishimura, Nahoko Kato-Kogoe, Yoshihiko Soga, Sho Matsushita, Keiji Nakasho, Koji Yamanegi, Naoko Yamada, Nobuyuki Terada, Shogo Takashiba

    Journal of Clinical Periodontology   35 ( 4 )   317 - 323   2008年4月

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    担当区分:最終著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/j.1600-051x.2008.01208.x

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  • Appearance of Multidrug-Resistant Opportunistic Bacteria on the Gingiva During Leukemia Treatment 査読

    Yoshihiko Soga, Takashi Saito, Fusanori Nishimura, Fumihiko Ishimaru, Junji Mineshiba, Fumi Mineshiba, Hirokazu Takaya, Hideaki Sato, Chieko Kudo, Susumu Kokeguchi, Nobuharu Fujii, Mitsune Tanimoto, Shogo Takashiba

    Journal of Periodontology   79 ( 1 )   181 - 186   2008年1月

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    担当区分:最終著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1902/jop.2008.070205

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  • 歯周病細菌感染度診断のための血清IgG抗体価検査の臨床的有用性-血清バンク(バイオバンクジャパン)試料での検討-

    久枝 綾, 成石 浩司, 工藤 値英子, 安孫子 宣光, 小方 頼昌, 島内 英俊, 長澤 敏行, 永田 俊彦, 沼部 幸博, 野口 俊英, 日野 孝宗, 村上 伸也, 山崎 和久, 吉村 篤利, 新井 英雄, 高柴 正悟

    特定非営利活動法人 日本歯周病学会学術大会 プログラムおよび講演抄録集   2008   126 - 126   2008年

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    記述言語:日本語   出版者・発行元:特定非営利活動法人 日本歯周病学会  

    DOI: 10.14833/amjsp.2008s.0.126.0

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  • ある侵襲性歯周炎患者の歯周病治療の経過と血清IgG抗体価の関連性の検討:症例報告

    内藤 仁美, 工藤 値英子, 岩本 義博, 成石 浩司, 新井 英雄, 高柴 正悟

    特定非営利活動法人 日本歯周病学会学術大会 プログラムおよび講演抄録集   2008   128 - 128   2008年

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    記述言語:日本語   出版者・発行元:特定非営利活動法人 日本歯周病学会  

    DOI: 10.14833/amjsp.2008s.0.128.0

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  • 歯周病細菌感染度検査のための指尖血漿IgG抗体価の臨床的評価

    工藤 値英子, 成石 浩司, 久枝 綾, 安孫子 宣光, 小方 頼昌, 島内 英俊, 長澤 敏行, 永田 俊彦, 沼部 幸博, 野口 俊英, 日野 孝宗, 村上 伸也, 山崎 和久, 吉村 篤利, 新井 英雄, 高柴 正悟

    特定非営利活動法人 日本歯周病学会学術大会 プログラムおよび講演抄録集   2008   127 - 127   2008年

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    記述言語:日本語   出版者・発行元:特定非営利活動法人 日本歯周病学会  

    DOI: 10.14833/amjsp.2008s.0.127.0

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  • Smad2は歯肉上皮細胞の増殖抑制に関与する

    下江 正幸, 塩見 信行, 冨川 和哉, 峯柴 淳二, 新井 英雄, 高柴 正悟

    特定非営利活動法人 日本歯周病学会学術大会 プログラムおよび講演抄録集   2008   70 - 70   2008年

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    記述言語:日本語   出版者・発行元:特定非営利活動法人 日本歯周病学会  

    DOI: 10.14833/amjsp.2008s.0.70.0

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  • 歯周病患者における肥満およびその他の生活習慣と歯周病の関連性

    冨川 和哉, 目黒 道生, 畑中 加珠, 岩本 義博, 新井 英雄, 高柴 正悟

    特定非営利活動法人 日本歯周病学会学術大会 プログラムおよび講演抄録集   2008   92 - 92   2008年

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    記述言語:日本語   出版者・発行元:特定非営利活動法人 日本歯周病学会  

    DOI: 10.14833/amjsp.2008s.0.92.0

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  • 低濃度LPSにより脂肪細胞・マクロファージ共培養系から産生される分子のサイトカインアレイ解析

    山下 明子, 西村 英紀, 曽我 賢彦, 岩本 義博, 苔口 進, 高柴 正悟

    特定非営利活動法人 日本歯周病学会学術大会 プログラムおよび講演抄録集   2008   114 - 114   2008年

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    記述言語:日本語   出版者・発行元:特定非営利活動法人 日本歯周病学会  

    DOI: 10.14833/amjsp.2008s.0.114.0

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  • Aggregatibacter (Actinobacillus) actinomycetemcomitansからのsmall non-coding RNAならびにRNAシャペロンの同定

    前田 博史, 苔口 進, 谷本 一郎, 小出 康史, 園井 教裕, 山部 こころ, 新井 英雄, 高柴 正悟

    特定非営利活動法人 日本歯周病学会学術大会 プログラムおよび講演抄録集   2008   108 - 108   2008年

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    記述言語:日本語   出版者・発行元:特定非営利活動法人 日本歯周病学会  

    DOI: 10.14833/amjsp.2008s.0.108.0

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  • Focal adhesion kinase mediates human leukocyte histocompatibility antigen class II-induced signaling in gingival fibroblasts

    S. Yoshizawa, M. Meguro, H. Ohyama, K. Takeuchi-Hatanaka, S. Matsushita, S. Takashiba, F. Nishimura

    Journal of Periodontal Research   42 ( 6 )   572 - 579   2007年12月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/j.1600-0765.2007.00985.x

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  • Macrophage-Adipocyte Interaction: Marked Interleukin-6 Production by Lipopolysaccharide** 査読

    Akiko Yamashita, Yoshihiko Soga, Yoshihiro Iwamoto, Sayuri Yoshizawa, Hirotaka Iwata, Susumu Kokeguchi, Shogo Takashiba, Fusanori Nishimura

    Obesity   15 ( 11 )   2549 - 2552   2007年11月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1038/oby.2007.305

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  • cAMP-response element binding protein (CREB) regulates cyclosporine-A-mediated down-regulation of cathepsin B and L synthesis 査読

    Kazuhiro Omori, Koji Naruishi, Tomoko Yamaguchi, Shun-Ai Li, Mayumi Yamaguchi-Morimoto, Kaori Matsuura, Hideo Arai, Kohji Takei, Shogo Takashiba

    Cell and Tissue Research   330 ( 1 )   75 - 82   2007年9月

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    担当区分:最終著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    DOI: 10.1007/s00441-007-0457-8

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  • High glucose up-regulates lipopolysaccharide-stimulated inflammatory cytokine production via c-jun N-terminal kinase in the monocytic cell line THP-1 査読

    Hirotaka Iwata, Yoshihiko Soga, Michio Meguro, Sayuri Yoshizawa, Yuka Okada, Yoshihiro Iwamoto, Akiko Yamashita, Shogo Takashiba, Fusanori Nishimura

    Journal of Endotoxin Research   13 ( 4 )   227 - 234   2007年8月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE Publications  

    Diabetic subjects are susceptible to atherosclerosis. It has been postulated that inflammation plays a crucial role in atherogenesis. Since previous studies suggested persistent low-grade infection by Gram-negative bacteria such as Chlamydia spp. and/or periodontal infection is associated with increased atherogenesis among diabetic subjects, we hypothesized that macrophages under hyperglycemia respond to lipopolysaccharide (LPS) challenge in a more exaggerated manner than under normal glucose conditions. Therefore, we examined cytokine productivity and associated signal transduction molecules in LPS-stimulated the monocytic cell line THP-1, under conditions of hyperglycemia. Differentiated THP-1 cells were cultured under normal and high glucose conditions without fetal bovine serum, and were stimulated with Escherichia coli LPS in the presence of LPS binding protein. Following stimulation, activated signal transduction molecules were detected by protein microarray and confirmed thereafter. Results indicated that c-jun N-terminal kinase (JNK) was highly-phosphorylated at high glucose concentrations, and this was confirmed by Western-immunoblotting. Tumor necrosis factor-α and monocyte chemo-attractant protein-1 production were significantly enhanced under these conditions. SP600125, a selective inhibitor of JNK, dose-dependently suppressed the production of these cytokine. Therefore, we suggest that this may be one of the mechanisms by which sub-clinical infection by Gram-negative bacteria promotes atherosclerosis in diabetic subjects.

    DOI: 10.1177/0968051907082608

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  • Gene Profiles during Root Canal Treatment in Experimental Rat Periapical Lesions 査読

    Zulema Rosalia Arias Martinez, Koji Naruishi, Keisuke Yamashiro, Fumio Myokai, Teruo Yamada, Kaori Matsuura, Naoko Namba, Hideo Arai, Junzo Sasaki, Yoshimitsu Abiko

    Journal of Endodontics   33 ( 8 )   936 - 943   2007年8月

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    担当区分:最終著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.joen.2007.04.016

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  • Relationship of periodontal infection to serum antibody levels to periodontopathic bacteria and inflammatory markers in periodontitis patients with coronary heart disease 査読

    K. Yamazaki, T. Honda, H. Domon, T. Okui, K. Kajita, R. Amanuma, C. Kudoh, S. Takashiba, S. Kokeguchi, F. Nishimura, M. Kodama, Y. Aizawa, H. Oda

    Clinical & Experimental Immunology   149 ( 3 )   445 - 452   2007年7月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/j.1365-2249.2007.03450.x

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  • Oligonucleotide array analysis of cyclic tension-responsive genes in human periodontal ligament fibroblasts 査読

    Keisuke Yamashiro, Fumio Myokai, Koichi Hiratsuka, Tadashi Yamamoto, Kyoko Senoo, Hideo Arai, Fusanori Nishimura, Yoshimitsu Abiko, Shogo Takashiba

    The International Journal of Biochemistry & Cell Biology   39 ( 5 )   910 - 921   2007年1月

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    担当区分:最終著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.biocel.2007.01.015

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  • Regression of pustulosis palmaris et plantaris by periodontal treatment in a subject with severe periodontitis 査読

    Hiroshi Akazawa, Fushanori Nishimura, Hiroshi Maeda, Shogo Takashiba, Atsushi Mine, Kenji Maekawa, Takuo Kuboki

    International Journal of Dermatology   45 ( 12 )   1420 - 1422   2006年12月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/j.1365-4632.2006.02900.x

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  • Cloning and characterization of lipopolysaccharide-induced tumor necrosis factor α factor promoter 査読

    Nobuyuki Shiomi, Fumio Myokai, Koji Naruishi, Kosuke Oyaizu, Kyoko Senoo, Tomoko Yamaguchi, Salomon Amar, Shogo Takashiba

    FEMS Immunology & Medical Microbiology   47 ( 3 )   360 - 368   2006年8月

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    担当区分:最終著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

    DOI: 10.1111/j.1574-695x.2006.00094.x

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  • Gene polymorphisms in periodontal health and disease. 査読 国際誌

    Shogo Takashiba, Koji Naruishi

    Periodontology 2000   40 ( 1 )   94 - 106   2006年2月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/j.1600-0757.2005.00142.x

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  • The regulatory effect of fermentable sugar levels on the production of leukotoxin by Actinobacillus actinomycetemcomitans 査読

    Katsunori Mizoguchi, Hiroyuki Ohta, Atsushi Miyagi, Hidemi Kurihara, Shogo Takashiba, Keijiro Kato, Yoji Murayama, Kazuhiro Fukui

    FEMS Microbiology Letters   146 ( 1 )   161 - 166   2006年1月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

    DOI: 10.1111/j.1574-6968.1997.tb10187.x

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  • Anti-inflammatory effect of linear polarized infrared irradiation on interleukin-1β-induced chemokine production in MH7A rheumatoid synovial cells 査読

    Yasuko Shibata, Naomi Ogura, Keisuke Yamashiro, Shogo Takashiba, Toshirou Kondoh, Keiji Miyazawa, Masaru Matsui, Yoshimitsu Abiko

    Lasers in Medical Science   20 ( 3-4 )   109 - 113   2005年12月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    DOI: 10.1007/s10103-005-0350-1

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  • α2 Integrin +807 Polymorphism in Drug-induced Gingival Overgrowth 査読 国際誌

    M. Ogino, J. Kido, M. Bando, N. Hayashi, C. Wada, T. Nagata, F. Nishimura, Y. Soga, S. Takashiba, T. Kubota, M. Itagaki, Y. Shimada, H. Tai, H. Yoshie, N. Yamazaki, Y. Shinohara, M. Kataoka

    Journal of Dental Research   84 ( 12 )   1183 - 1186   2005年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE Publications  

    α2 integrin on fibroblasts is reported to play an important role in the induction of drug-induced gingival overgrowth, which is characterized by excessive accumulation of type I collagen in gingival connective tissue. Silent polymorphism 807 T/C within the α2 integrin gene is associated with high/low α2 integrin expression. The aim of this study was to test the hypothesis that expression of α2 integrin 807 T/C polymorphism correlates with drug-induced gingival overgrowth. A case-control study comparing 136 subjects taking calcium channel blockers (72 with vs. 64 without drug-induced gingival overgrowth) demonstrated that the frequency of the +807 C allele was significantly higher in the case group than in the controls (odds ratio, 3.61; 95% confidence interval, 2.14 – 6.10; P &lt; 0.05). The present findings suggest that the α2 +807 C allele is one of the genetic risk factors for drug-induced gingival overgrowth.

    DOI: 10.1177/154405910508401217

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  • Isolation and Expression of FIP-2 in Wounded Pulp of the Rat 査読 国際誌

    M. Oyama, F. Myokai, T. Ohira, N. Shiomi, K. Yamashiro, H. Arai, F. Nishimura, S. Takashiba

    Journal of Dental Research   84 ( 9 )   842 - 847   2005年9月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE Publications  

    Pulpal wound healing followed by cavity preparation may involve reactionary or reparative dentinogenesis in relation to the cavity position; however, little is known about the molecular responses. We aimed to isolate and analyze genes induced or suppressed in the wounded pulp to identify molecular processes involved in the pulp responses to injury. Twenty-three cDNAs were isolated by cDNA subtraction between healthy and wounded pulp of rats. By library screening, we identified rat 14.7K-interacting protein (rFIP)-2A and B genes homologous to human FIP-2, being involved in regulating membrane trafficking and cellular morphogenesis. RT-PCR analysis showed induction for only rFIP-2B in the wounded pulp. In situ hybridization analysis revealed that both rFIP-2s were expressed strongly in condensing mesenchymal cells of the palatal process and surrounding Meckel’s cartilage, but not in intramembranous chondrogenic cells. Thus, up-regulated rFIP-2B expression may play a role in regulating membrane trafficking or cellular morphogenesis of these embryonic and wounded pulpal cells.

    DOI: 10.1177/154405910508400912

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  • Transcriptional regulation of β-defensin-2by lipopolysaccharide in cultured human cervical carcinoma (HeLa) cells 査読

    Junji Mineshiba, Fumio Myokai, Fumi Mineshiba, Kaori Matsuura, Fusanori Nishimura, Shogo Takashiba

    FEMS Immunology & Medical Microbiology   45 ( 1 )   37 - 44   2005年7月

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    担当区分:最終著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

    DOI: 10.1016/j.femsim.2005.01.008

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  • Periodontal Treatment in Severe Aplastic Anemia 査読 国際誌

    Kosuke Oyaizu, Fumi Mineshiba, Junji Mineshiba, Hirokazu Takaya, Fusanori Nishimura, Ichiro Tanimoto, Hideo Arai, Shogo Takashiba

    Journal of Periodontology   76 ( 7 )   1211 - 1216   2005年7月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    Background: Aplastic anemia (AA) is a rare hematologic disease characterized by hypo-cellular bone marrow. The clinical features include fatigue, increased bruising, and gingival bleeding caused by anemia, leukopenia, and thrombocytopenia. A patient with AA is at high risk for infection because of leukopenia. The risk of systemic infection is especially high in AA patients with severe local infections, including periodontitis. Accordingly, periodontal treatment should include antibiotic prophylaxis to reduce the risk of systemic infection. However, treatment of periodontitis in the AA patient is significantly complicated by the bleeding disorder. We present a case report of the successful periodontal treatment of an AA patient with spontaneous gingival bleeding.
    Methods: The patient was closely monitored for platelet and neutrophil counts before every treatment. The patient's platelet count was always under 10,000/mu l. Therefore, it was necessary to increase platelet counts to over 25,000/mu l by transfusion, after which subgingival scaling with anesthesia was performed. When the neutrophil count was less than 2,000/mu l, local minocycline chemotherapy was applied to the pockets. Periodontal infection was monitored by detection of bacterial DNA and measurement of serum immunoglobulin (Ig) G titer against periodontal bacteria.
    Results: Following the physical and chemical treatment, the gingival appearance improved dramatically and the spontaneous gingival bleeding disappeared. Moreover, the IgG titer against periodontal bacteria decreased to normal range and specific periodontal pathogens were no longer detectable in the tested pockets.
    Conclusion: We believe that the treatment strategy in the present report provides new sight into treatment planning for severely medically compromised patients.

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  • Role of helper T cells in the humoral immune responses against 53-kDa outer membrane protein from Porphyromonas gingivalis 査読

    N. Kato, H. Ohyama, F. Nishimura, S. Matsushita, S. Takashiba, Y. Murayama

    Oral Microbiology and Immunology   20 ( 2 )   112 - 117   2005年4月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/j.1399-302x.2004.00203.x

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  • Thiazolidinedione (Pioglitazone) Blocks P. gingivalis- and F. nucleatum, but not E. coli, Lipopolysaccharide (LPS)-induced Interleukin-6 (IL-6) Production in Adipocytes 査読 国際誌

    M. Yamaguchi, F. Nishimura, H. Naruishi, Y. Soga, S. Kokeguchi, S. Takashiba

    Journal of Dental Research   84 ( 3 )   240 - 244   2005年3月

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    担当区分:最終著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE Publications  

    An elevated level of C-reactive protein (CRP) predicts the future development of coronary heart disease. Periodontitis appears to up-regulate CRP. CRP is produced by hepatocytes in response to interleukin-6 (IL-6). A major source of IL-6 in obese subjects is adipocytes. We hypothesized that lipopolysaccharide (LPS) from periodontal pathogens stimulated adipocytes to produce IL-6, and that the production was suppressed by the drugs targeted against insulin resistance, thiazolidinedione (pioglitazone), since this agent potentially showed an anti-inflammatory effect. Mouse 3T3-L1 adipocytes were stimulated with E. coli, P. gingivalis, and F. nucleatum LPS. The IL-6 concentration in culture supernatants was measured. All LPS stimulated adipocytes to produce IL-6. Although pioglitazone changed adipocyte appearance from large to small, and completely suppressed P. gingivalis and F. nucleatum LPS-induced IL-6 production, E. coli LPS-induced IL-6 production was not efficiently blocked. Thus, pioglitazone completely blocked periodontal-bacteria-derived LPS-induced IL-6 production in adipocytes, a major inducer of CRP.

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  • Detection of periodontal pathogenPorphyromonas gingivalisby loop-mediated isothermal amplification method 査読

    Hiroshi Maeda, Susumu Kokeguchi, Chiyo Fujimoto, Ichiro Tanimoto, Wakako Yoshizumi, Fusanori Nishimura, Shogo Takashiba

    FEMS Immunology & Medical Microbiology   43 ( 2 )   233 - 239   2005年2月

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    担当区分:最終著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

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  • Long-term cyclosporin A exposure suppresses cathepsin-B and -L activity in gingival fibroblasts. 査読 国際誌

    Mayumi Yamaguchi, Koji Naruishi, Hisa Yamada-Naruishi, Kazuhiro Omori, Fusanori Nishimura, Shogo Takashiba

    Journal of periodontal research   39 ( 5 )   320 - 6   2004年10月

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    担当区分:最終著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    BACKGROUND: Gingival overgrowth is a common side-effect following administration of cyclosporin A. We reported previously that lysosomal protease cathepsin-L activity, but not cathepsin-B, was significantly suppressed by short-term cyclosporin A exposure in human gingival fibroblasts. Although this suppression may lead to decreased degradation of gingival connective tissue, a net increase in matrix proteins, and gingival overgrowth, the effects of cyclosporin A need to be more elucidated, considering the long-term use for patients following organ transplantation. OBJECTIVE: The aim of the present study was to evaluate the effects of clinically relevant doses of cyclosporin A on cultured human gingival fibroblasts. We evaluated the effects of long-term cyclosporin A exposure on cell proliferation, mRNA expression of various proteases and both cathepsin-B and -L activity in human gingival fibroblasts. MATERIALS AND METHODS: Human gingival fibroblasts were isolated from three donors' healthy gingiva and cultured from five to eight passages with or without 200 ng/ml of cyclosporin A. Proliferative activity of cyclosporin A-treated cells was examined using MTT assay. Total RNA and cellular proteins were collected for semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis and for measurement of the cathepsin-B and -L activity. RESULTS: Long-term cyclosporin A exposure had no effects on cell proliferation. Accumulation of cathepsin-B, -H and -L mRNA was markedly suppressed by long-term cyclosporin A exposure, whereas accumulation of another lysosomal enzyme N-acetyl-beta-D-glucosaminidase mRNA, which is involved in remodeling of gingival epithelium, was not apparently impaired in cyclosporin A-treated cells. Accumulation of matrix metalloprotease-1 (MMP-1) and tissue inhibitor of matrix metalloprotease-1 (TIMP-1) mRNA, which are involved in remodeling of extracellular matrix, also was not impaired. In addition, we demonstrated that long-term cyclosporin A exposure significantly suppressed not only the activity of the active form of cathepsin-(B + L) compared to the activity in non-treated cells (p = 0.0458), but also the activity of the active form of cathepsin-B (p < 0.0001) in human gingival fibroblasts. CONCLUSION: The decreased ability of protein degradation by not only cathepsin-L but also cathepsin-B is, at least, one of the several factors developing the cyclosporin A-induced gingival overgrowth.

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  • Identification of Genes Differentially Regulated in Rat Alveolar Bone Wound Healing by Subtractive Hybridization 査読 国際誌

    T. Ohira, F. Myokai, N. Shiomi, K. Yamashiro, T. Yamamoto, Y. Murayama, H. Arai, F. Nishimura, S. Takashiba

    Journal of Dental Research   83 ( 7 )   546 - 551   2004年7月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE Publications  

    Periodontal healing requires the participation of regulatory molecules, cells, and scaffold or matrix. Here, we hypothesized that a certain set of genes is expressed in alveolar bone wound healing. Reciprocal subtraction gave 400 clones from the injured alveolar bone of Wistar rats. Identification of 34 genes and analysis of their expression in injured tissue revealed several clusters of unique gene regulation patterns, including the up-regulation at 1 wk of cytochrome c oxidase regulating electron transfer and energy metabolism, presumably occurring at the site of inflammation; up-regulation at 2.5 wks of pro-α-2 type I collagen involving the formation of a connective tissue structure; and up-regulation at 1 and 2 wks and down-regulation at 2.5 and 4 wks of ubiquitin carboxyl-terminal hydrolase l3 involving cell cycle, DNA repair, and stress response. The differential expression of genes may be associated with the processes of inflammation, wound contraction, and formation of a connective tissue structure.

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  • High Glucose Enhances Interleukin-6-induced Vascular Endothelial Growth Factor 165 Expression via Activation of Gp130-mediated p44/42 MAPK-CCAAT/Enhancer Binding Protein Signaling in Gingival Fibroblasts 査読

    Kazuhiro Omori, Koji Naruishi, Fusanori Nishimura, Hisa Yamada-Naruishi, Shogo Takashiba

    Journal of Biological Chemistry   279 ( 8 )   6643 - 6649   2004年2月

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    担当区分:最終著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1074/jbc.m311688200

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  • Systemic up-regulation of sTNFR2 and IL-6 in Porphyromonas gingivalis pneumonia in mice 査読

    Milan Petelin, Koji Naruishi, Nobuyuki Shiomi, Junji Mineshiba, Hideo Arai, Fusanori Nishimura, Shogo Takashiba, Yoji Murayama

    Experimental and Molecular Pathology   76 ( 1 )   76 - 81   2004年2月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.yexmp.2003.09.002

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  • CYP2C polymorphisms, phenytoin metabolism and gingival overgrowth in epileptic subjects 査読

    Yoshihiko Soga, Fusanori Nishimura, Yoko Ohtsuka, Hiroaki Araki, Yoshihiro Iwamoto, Hisa Naruishi, Nobuyuki Shiomi, Yoshitomo Kobayashi, Shogo Takashiba, Kenji Shimizu, Yutaka Gomita, Eiji Oka

    Life Sciences   74 ( 7 )   827 - 834   2004年1月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.lfs.2003.07.018

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  • C-Jun N-terminal kinase (JNK) inhibitor, SP600125, blocks interleukin (IL)–6-induced vascular endothelial growth factor (VEGF) production: cyclosporine A partially mimics this inhibitory effect 査読

    Koji Naruishi, Fusanori Nishimura, Hisa Yamada-Naruishi, Kazuhiro Omori, Mayumi Yamaguchi, Shogo Takashiba

    Transplantation   76 ( 9 )   1380 - 1382   2003年11月

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    担当区分:責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Ovid Technologies (Wolters Kluwer Health)  

    DOI: 10.1097/01.tp.0000085661.52980.95

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  • Application of denaturing gradient gel electrophoresis (DGGE) to the analysis of microbial communities of subgingival plaque 査読

    C. Fujimoto, H. Maeda, S. Kokeguchi, S. Takashiba, F. Nishimura, H. Arai, K. Fukui, Y. Murayama

    Journal of Periodontal Research   38 ( 4 )   440 - 445   2003年8月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1034/j.1600-0765.2003.02607.x

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  • Gene Profiling in Human Periodontal Ligament Fibroblasts by Subtractive Hybridization 査読

    T. Yamamoto, F. Myokai, F. Nishimura, T. Ohira, N. Shiomi, K. Yamashiro, H. Arai, Y. Murayama, S. Takashiba

    Journal of Dental Research   82 ( 8 )   641 - 645   2003年8月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE Publications  

    Genes expressed by human periodontal ligament fibroblasts (HPFs) are likely to be associated with specific functions of the ligament. The aim of this study is to profile genes expressed highly by HPFs. A library (6 × 103pfu) was constructed, followed by subtraction of HPF cDNAs with human gingival fibroblast (HGF) cDNAs. Reverse-dot hybridization revealed that 33 clones expressed higher levels of specific mRNAs in HPFs than in HGFs. These were mRNAs for known genes, including several associated with maturation and differentiation of cells. None had been reported in PFs. One clone, PDL-29, identified as a COX assembly factor, showed much stronger mRNA expression in HPFs than in HGFs in culture. In rat periodontium, however, PDL-29 mRNA expression was similar in PFs and GFs. These results suggest that HPFs express many previously unreported genes associated with maturation and differentiation, but expression can differ in vitro and in vivo.

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  • Antimicrobial Periodontal Treatment Decreases Serum C-Reactive Protein, Tumor Necrosis Factor-Alpha, But Not Adiponectin Levels in Patients with Chronic Periodontitis 査読

    Yoshihiro Iwamoto, Fusanori Nishimura, Yoshihiko Soga, Kazu Takeuchi, Mikinao Kurihara, Shogo Takashiba, Yoji Murayama

    Journal of Periodontology   74 ( 8 )   1231 - 1236   2003年8月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1902/jop.2003.74.8.1231

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  • Tumor necrosis factor-alpha gene (TNF-α) −1031/−863, −857 single-nucleotide polymorphisms (SNPs) are associated with severe adult periodontitis in Japanese 査読 国際誌

    Yoshihiko Soga, Fusanori Nishimura, Hideki Ohyama, Hiroshi Maeda, Shogo Takashiba, Yoji Murayama

    Journal of Clinical Periodontology   30 ( 6 )   524 - 531   2003年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    Objectives: Tumour necrosis factor-alpha (TNF-alpha ) and interleukin-1beta (IL-1beta ) participate in the establishment of inflammatory lesions in periodontitis. High production of these cytokines may relate to the severity of periodontitis. There have already been several studies examining the association between periodontitis and single nucleotide polymorphisms (SNPs) that affect cytokine productivity. Recently, new SNPs of TNF-alpha , -1031, -863 and -857, variants of which are observed in a relatively large proportion in Japanese, have been identified. The variant alleles of these SNPs have been suggested to be related to high TNF-alpha production. For a better understanding of the genetic factors associated with the severity of periodontitis, further analysis including these newly identified SNPs is essential. In addition, previous reports on TNF-alpha or IL-1beta SNPs associated with periodontitis were mainly for Caucasian populations. Therefore, the aim of this study is to examine the association between severe periodontitis in Japanese and the following SNPs: five in the TNF-alpha gene promoter (-1031, -863, -857, -308, -238) and three in the IL-1beta gene (-511, -31, +3953).
    Material and Methods: A total of 128 Japanese individuals were enrolled in this study. They were 64 patients with severe adult periodontitis and 64 healthy subjects. TNF-alpha and IL-1beta SNPs were genotyped by polymerase chain reaction-restriction fragment length polymorphism for all subjects. TNF-alpha and IL-1beta production from LPS-stimulated monocytes/macrophages was also measured for 15 healthy male subjects.
    Results: TNF-alpha production in TNF-alpha -1031/-863 (linkage disequilibrated) or -857 SNP variant allele carriers tended to be elevated, and the frequency of subjects who carried at least one variant allele in TNF-alpha -1031, -863 or -857 SNPs among severe periodontitis patients was significantly higher than in healthy subjects.
    Conclusion: Since the frequency of subjects who carried at least one variant allele in TNF-alpha -1031, -863 or -857 SNPs was higher in periodontitis patients than in healthy subjects, TNF-alpha -1031, -863 and -857 SNPs appear to be associated with severe adult periodontitis in Japanese populations.

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  • Unique genes induced by mechanical stress in periodontal ligament cells 査読 国際誌

    Fumio Myokai, Masataka Oyama, Fusanori Nishimura, Taisuke Ohira, Tadashi Yamamoto, Hideo Arai, Shogo Takashiba, Yoji Murayama

    Journal of Periodontal Research   38 ( 3 )   255 - 261   2003年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    Objectives: The aim of this study is to isolate mechanical stress-induced genes (MSGens) from human periodontal ligament (PDL) cells and to analyze profiles of the mRNA expression of these genes.
    Background: Differential expression of genes in PDL cells under physiological stress such as occlusal force is thought to be orchestrated not only for the remodeling of PDL itself but also for the repair and regeneration of periodontal tissues. However, little is known about the genes expressed in PDL cells under mechanical stress.
    Methods: The cDNA from mechanical stress-applied human PDL cells was subtracted against the cDNA from static control cells. The subtracted cDNA was amplified by polymerase chain reaction (PCR) and cloned for further analysis.
    Results: Among 68 independent clones isolated, 15 contained DNA fragments greater than 250 bp. Reverse Northern analysis revealed a marked induction of MSGen-15 and MSGen-28 mRNA expression in the mechanical stress-applied cells. However, little difference in the magnitude of expression for the other MSGens was detected between the stress-applied cells and the control cells. After nucleotide sequencing and the analysis of homology with known genes, five clones were identified; ribosomal protein S27 (MSGen-9), MRG 15 (MSGen-15), androgen-binding protein (MSGen-18), cathepsin H (MSGen-28), and cytochrome c (MSGen-47). Interestingly, it has been reported that MRG 15 is a novel transcription factor involved in the regulation of cell growth and senescence. The remaining 10 clones, classified into six sequence types, had no significant homology with any known genes.
    Conclusions: These results suggest that many known and unknown genes are expressed in response to mechanical stress in PDL cells, and that a transcription factor, MRG 15, may be responsible for molecular events in PDL cells under mechanical stress.

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  • Ligation of IFN-γ-induced HLA-DR molecules on fibroblasts induces RANTES expression via c-Jun N-terminal kinase (JNK) pathway 査読

    Michio Meguro, Fusanori Nishimura, Hideki Ohyama, Shogo Takashiba, Yoji Murayama, Sho Matsushita

    Cytokine   22 ( 5 )   107 - 115   2003年6月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/s1043-4666(03)00123-6

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  • Antibacterial activity of synthetic human B defensin-2 against periodontal bacteria. 査読 国際誌

    Mineshiba F, Takashiba S, Mineshiba J, Matsuura K, Kokeguchi S, Murayama Y

    Journal of the International Academy of Periodontology   5 ( 2 )   35 - 40   2003年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:2  

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  • Perspective of Cytokine Regulation for Periodontal Treatment: Fibroblast Biology 査読

    Shogo Takashiba, Koji Naruishi, Yoji Murayama

    Journal of Periodontology   74 ( 1 )   103 - 110   2003年1月

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    担当区分:筆頭著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

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  • COUNTER-ANTIGEN PRESENTATION: FIBROBLASTS PRODUCE CYTOKINES BY SIGNALLING THROUGH HLA CLASS II MOLECULES WITHOUT INDUCING T-CELL PROLIFERATION 査読

    Hideki Ohyama, Fusanori Nishimura, Michio Meguro, Shogo Takashiba, Yoji Murayama, Sho Matsushita

    Cytokine   17 ( 4 )   175 - 181   2002年2月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

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  • T cell responses to major membrane protein II (MMP II) of Mycobacterium leprae are restricted by HLA-DR molecules in patients with leprosy 査読

    Hideki Ohyama, Sho Matsushita, Fusanori Nishimura, Nahoko Kato, Kentaro Hatano, Shogo Takashiba, Yoji Murayama

    Vaccine   20 ( 3-4 )   475 - 482   2001年11月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/s0264-410x(01)00354-1

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  • Subgingival microflora and antibody responses against periodontal bacteria of young Japanese patients with type 1 diabetes mellitus. 査読 国際誌

    Takahashi K, Nishimura F, Kurihara M, Iwamoto Y, Takashiba S, Miyata T, Murayama Y

    Journal of the International Academy of Periodontology   3 ( 4 )   104 - 111   2001年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:4  

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  • Characterization of Two Genes Encoding Ferritin-Like Protein inActinobacillus actinomycetemcomitans 査読

    Masaru Hirosue, Susumu Kokeguchi, Hiroshi Maeda, Fusanori Nishimura, Shogo Takashiba, Yoji Murayama

    Microbiology and Immunology   45 ( 10 )   721 - 727   2001年10月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/j.1348-0421.2001.tb01307.x

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  • Impairment of Gingival Fibroblast Adherence by IL-6/sIL-6R 査読 国際誌

    K. Naruishi, S. Takashiba, F. Nishimura, H.-H. Chou, H. Arai, H. Yamada, Y. Murayama

    Journal of Dental Research   80 ( 5 )   1421 - 1424   2001年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE Publications  

    Interleukin-6 (IL-6) binds to human gingival fibroblasts (HGF) in the presence of a soluble form of IL-6 receptor (sIL-6R). We investigated the effects of IL-6 on the functions of HGF in the presence of sIL-6R. HGF changed their morphology from spindle-shaped to round, and detached from the culture dish by stimulation with IL-6/sIL-6R. In this condition, a signal transducer gpl30 and a transcription factor Stat3 were phosphorylated, resulting in activation of transcription factors Stat3 and C/EBPβ. Cytoskeletal β-actin and adhesion molecule integrin-a5, a subunit of α5β1 integrin (VLA-5), were found to possess potential binding domains for these transcription factors in their promoters. Accumulation of (3-actin and integrin-a5 mRNA decreased, contrary to the expectation of the induction of gene transcription. Furthermore, the decrease in their mRNAs was associated with reduced expression of both actin and VLA-5 proteins. These results suggest that the expression of VLA-5 and actin was down-regulated in HGF through an IL-6 signaling pathway, resulting in impairment of HGF adherence.

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  • Identification and characterization of B-cell epitopes of a 53-kDa outer membrane protein from Porphyromonas gingivalis 査読

    K. Oyaizu, H. Ohyama, F. Nishimura, H. Kurihara, S. Matsushita, H. Maeda, S. Kokeguchi, H. Hongyo, S. Takashiba, Y. Murayama

    Oral Microbiology and Immunology   16 ( 2 )   73 - 78   2001年4月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1034/j.1399-302x.2001.016002073.x

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  • Serum phenytoin concentration and IgG antibody titre to periodontal bacteria in patients with phenytoin-induced gingival overgrowth. 査読 国際誌

    Yamada H, Nishimura F, Furuno K, Naruishi K, Kobayashi Y, Takashiba S, Murayama Y

    Journal of the International Academy of Periodontology   3 ( 2 )   42 - 47   2001年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:2  

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  • Heterogeneity of Host Immunological Risk Factors in Patients With Aggressive Periodontitis 査読

    Keiso Takahashi, Hideki Ohyama, Michitaka Kitanaka, Takamasa Sawa, Junji Mineshiba, Fusanori Nishimura, Hideo Arai, Shogo Takashiba, Yoji Murayama

    Journal of Periodontology   72 ( 4 )   425 - 437   2001年4月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

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  • Immunopathological Diagnosis of Cicatricial Pemphigoid With Desquamative Gingivitis. A Case Report 査読

    Mikinao Kurihara, Fusanori Nishimura, Takashi Hashimoto, Ayako Komai, Hiroyoshi Ueda, Susumu Kokeguchi, Shogo Takashiba, Yoji Murayama

    Journal of Periodontology   72 ( 2 )   243 - 249   2001年2月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

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  • Tumor Necrosis Factor-α (TNF-α)-Induced and Interleukin-1β (IL-1β)-Induced Shedding of TNF Receptors from Gingival Fibroblasts 査読

    Hyogo Ohe, Shogo Takashiba, Koji Naruishi, Hsin-Hua Chou, Hisa Yamada, Fusanori Nishimura, Hideo Arai, Yoji Murayama

    Journal of Interferon & Cytokine Research   20 ( 12 )   1077 - 1082   2000年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Mary Ann Liebert Inc  

    Tumor necrosis factor-alpha (TNF-alpha) exerts its functions by binding two different receptors (TNFR55 and TNFR75), Both TNFR55 and TNFR75 exist in cell-associated and soluble forms. Soluble TNF receptors (sTNFR), sTNFR55 and sTNFR75, are proteolytically shed upon inflammatory stimuli and then modulate various TNF-alpha bioactivities. As human gingival fibroblasts (HGF) can be potential targets for TNF-alpha in inflamed gingiva, we hypothesized that HGF partially modulate the cellular responses to TNF-alpha by regulating their own TNFR. In this study, the kinetics of expression of cell-associated and soluble forms of both receptors from cultured HGF in response to proinflammatory cytokines TNF-alpha and interleukin-1 beta (IL-1 beta) were investigated in vitro. Both TNF-alpha and IL-1 beta upregulated the gene expression of TNFR75 and did not affect that of TNFR55. TNF-alpha and IL-1 beta decreased binding of [I-125]TNF-alpha to HGF. Moreover, TNF-alpha and IL-1 beta upregulated the release of sTNFR75 from HGF but not that of sTNFR55, These results suggest that HGF under inflammatory conditions may contribute to the inactivation of circulating TNF-a through the preferential induction and shedding of TNFR75.

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  • Molecular characterization of the hlyX-like gene of Actinobacillus actinomycetemcomitans Y4 査読 国際誌

    Susumu Kokeguchi, Masaru Hirosue, Hiroshi Maeda, Manabu Miyamoto, Shogo Takashiba, Yoji Murayama

    Research in Microbiology   151 ( 9 )   721 - 725   2000年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    We isolated and characterized a possible regulatory gene, designated actX gene, from Actinobacillus actinomyctemcomitans Y4, which defined the Actinobacillus pleuropneumoniae hlyX-like regulatory gene. DNA sequence analysis for plasmid clone pKM317 containing a 1.6-kb DNA insert indicated an open reading frame encoding a polypeptide of 257 amino acid residues. Analysis of the deduced amino acid sequence showed the presence of five characteristic cysteine residues in the N-terminal region and a putative DNA binding residue in the C-terminal region, indicating that actX might belong to a regulatory gene family. Escherichia coli DH5 alpha and a mutant strain JRG1728 transformed by plasmid carrying actX manifested apparent hemolytic activity on sheep blood agar and grew anaerobically, although the original strains did not. (C) 2000 Editions scientifiques et medicales Elsevier SAS.

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  • Antibody responses against Porphyromonas gingivalis infection in patients with early-onset periodontitis 査読 国際誌

    Shujuan Guo, Keiso Takahashi, Susumu Kokeguchi, Shogo Takashiba, Denis F. Kinane, Yoji Murayama

    Journal of Clinical Periodontology   27 ( 10 )   769 - 777   2000年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    Background, aims: The aim of this study was to evaluate antibody responses against Porphyromonas gingivalis (P. gingivalis) infection in early-onset periodontitis (EOP) patients to elucidate further the host-parasite interactions in the pathogenesis of EOP.
    Method: 16 P. gingivalis-infected EOP and 20 adult periodontitis (AP) patients, and 18 periodontally healthy subjects (HS) participated in this study. Serum immunoglobulin G (IgG) antibody levels and avidities against extracted P. gingivalis whole cells were measured. The components of P. gingivalis outer membrane antigens (OMA) reacting to patients' sera were analysed from the molecular weights by Western blotting. Serum antibody levels against P. gingivalis lipopolysaccharide (LPS) were also measured. The ability of the patients' sera to block interleukin-1 beta (IL-1 beta) production by human mononuclear cells in response to P. gingivalis LPS was examined.
    Results: Antibody levels were positively correlated with antibody avidities in both EOP and AP patients (r=0.91, r=0.72, p&lt;0.0005, respectively), while not significantly so in HS (r=0.09). There was variability in the antigen recognition of P. gingivalis OMA in EOP and AP patients. Smear and 53-kDa protein were more frequently recognized by sera of EOP and AP patients rather than that of HS (p&lt;0.05). The smear was partly diminished by absorption with P. gingivalis LPS, indicating the smear antigen was partly composed of LPS. There was high correlation between antibody levels against P. gingivalis whole-cell extracts and LPS in EOP and AP patients (r=0.81, p=0.0002, r=0.87, p&lt;0.0001, respectively), while not significant in HS (r=0.22). The sera of EOP and AP patients with high IgG titre to P. gingivalis LPS blocked IL-1 beta production more effectively than that of the patients with low IgG titre to P. gingivalis LPS.
    Conclusions: These results indicate that EOP patients' antibody response against P. gingivalis infection does not differ significantly from that of AP patients. The person-to-person heterogeneous antibody production against P. gingivalis LPS could contribute to our understanding of the relationship between the defensive ability of EOP patients and their chronic infection with this pathogen.

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  • Induction of Intracellular Interleukin-1 β Signals via Type II Interleukin-1 Receptor in Human Gingival Fibroblasts 査読 国際誌

    H.-H. Chou, S. Takashiba, H. Maeda, K. Naruishi, F. Nishimura, H. Arai, H.-k. Lu, Y. Murayama

    Journal of Dental Research   79 ( 9 )   1683 - 1688   2000年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE Publications  

    The type II interleukin-1 receptor (IL-1RII) has been thought to be incapable of transducing signals to cells because of its short intracellular domain, while type I IL-1 receptor (IL-1RI) does transduce signals. Since over-expression of IL-1RII has been demonstrated to inhibit cytokine production in the fibroblastic cell line, it has been proposed to use IL-1RII to prevent IL-1-induced inflammation in connective tissue. In this study, trace amounts of IL-1RII mRNA expression were detected in human gingival fibroblasts (HGFs), which are affected by cytokines in inflammatory periodontal disease. Cloning of the cDNA encoding IL-1RII expressed in HGFs revealed 3 amino acid substitutions in the extracellular domain, when compared with the 408 residues predicted from human B-cells. Over-expression of IL-1RII on HGFs by gene transfer down-regulated the expression of IL-1β mRNA and IL-6 mRNA in response to IL-1β stimulation, while the expression of IL-8 mRNA was not affected. In the IL-1RII-transfected HGFs, phosphorylation of 25-and 74-kDa proteins was up-regulated upon IL-1β stimulation in the transfected HGFs. The phosphorylation of these proteins was suppressed by the addition of a neutralizing antibody against IL-1RII. These results suggest that the IL-1RII may regulate HGFs expression of cytokine mRNA upon IL-1β stimulation, possibly by altering the IL-1RI-dependent signals.

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  • High Glucose Suppresses Cathepsin Activity in Periodontal-ligament-derived Fibroblastic Cells 査読 国際誌

    F. Nishimura, K. Naruishi, H. Yamada, T. Kono, S. Takashiba, Y. Murayama

    Journal of Dental Research   79 ( 8 )   1614 - 1617   2000年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE Publications  

    The accumulation of extracellular matrices and integrins by high glucose has been reported in relation to diabetic complications. We previously reported that PDL cells expressed a higher amount of VLA-5 when cultured in high-glucose (4500 mg/L) medium than those cultured in low-glucose (1100 mg/L) medium. In this study, we aimed to address (1) whether this effect was mediated by the transcriptional level of the gene or the degradative level of the protein, and (2) whether this effect was mediated by TGF-β. The results indicated that the level of mRNA encoding a5 integrin did not change in PDL cells regardless of the concentration of glucose. Alternatively, high glucose suppressed cathepsin B+L activity. Additionally, the level of mRNA encoding TGF-β was not affected by high glucose, nor did an anti-TGF-β neutralizing antibody have an effect on the expression of β5 gene or cathepsin activity. Therefore, the effects of high glucose appeared to be mediated by impaired protein degradation, but not by autocrine TGF-β.

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  • Epitope mapping of heat shock protein 60 (GroEL) fromPorphyromonas gingivalis 査読

    Hiroshi Maeda, Manabu Miyamoto, Susumu Kokeguchi, Takayuki Kono, Fusanori Nishimura, Shogo Takashiba, Yoji Murayama

    FEMS Immunology & Medical Microbiology   28 ( 3 )   219 - 224   2000年7月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

    DOI: 10.1111/j.1574-695x.2000.tb01480.x

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  • Development of 16S rDNA-based PCR assay for detecting Centipeda periodontii and Selenomonas sputigena 査読

    S. Sawada, S. Kokeguchi, S. Takashiba, Y. Murayama

    Letters in Applied Microbiology   30 ( 6 )   423 - 426   2000年6月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1046/j.1472-765x.2000.00735.x

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  • Phenytoin and Cyclosporin A Suppress the Expression of MMP-1, TIMP-1, and Cathepsin L, But Not Cathepsin B in Cultured Gingival Fibroblasts 査読 国際誌

    Hisa Yamada, Fusanori Nishimura, Koji Naruishi, Hsin-Hua Chou, Shogo Takashiba, George M. Albright, Salvador Nares, Anthony M. Iacopino, Yoji Murayama

    Journal of Periodontology   71 ( 6 )   955 - 960   2000年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    Background: Fibroblasts are known not only to synthesize and secrete extracellular matrix proteins, but also to degrade them for connective tissue remodeling. Drug-induced gingival overgrowth is characterized by a massive accumulation of extracellular matrix components in gingival connective tissues. Although some previous reports suggested that causative drugs stimulated the fibroblast proliferation, the results are not conclusive yet. In this study, we hypothesized that drug-induced gingival overgrowth could be a consequence of impaired ability of matrix degradation rather than an enhanced proliferation of gingival fibroblasts induced by these drugs.
    Methods: Normal human gingival fibroblasts were cultured with or without either 20 mug/ml of phenytoin or 200 ng/ml of cyclosporin A. Total RNA and cellular proteins were collected every day for RT-PCR analyses and for measuring lysosomal enzyme activity. In addition, an immunohistochemical study was performed to detect lysosomal enzymes in cells from enlarged gingiva of the patients with phenytoin-induced gingival overgrowth.
    Results: RT-PCR analyses revealed that these drugs suppressed the expression of MMP-1, TIMP-1, and cathepsin L, but not that of cathepsin B in a time-dependent manner. Then, we measured the activity of lysosomal enzymes and cathepsin B and L. The results indicated that although cathepsin B activity was not observed to be impaired, regardless of the drugs used in these cells, both total and active forms of combined activity of cathepsins B and L were suppressed in a time-dependent manner.
    Conclusions: The results indicate that, besides suggested effects of these drugs on gingival fibroblasts and/or on accumulated cells in the gingival tissues, extracellular matrix-degrading ability, particularly that by cathepsin L, is also suppressed by cyclosporin A and phenytoin in gingival fibroblasts, and that lysosomal enzyme plays an important role in the pathogenesis of drug-induced gingival hyperplasia.

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  • Cell Surface-Associated Enolase inActinobacillus actinomycetemcomitans 査読

    Hiroaki Hara, Hiroyuki Ohta, Tetsuyoshi Inoue, Toshio Ohashi, Shogo Takashiba, Yoji Murayama, Kazuhiro Fukui

    Microbiology and Immunology   44 ( 5 )   349 - 356   2000年5月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/j.1348-0421.2000.tb02505.x

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  • Differentiation of Monocytes to Macrophages Primes Cells for Lipopolysaccharide Stimulation via Accumulation of Cytoplasmic Nuclear Factor κB 査読 国際誌

    Shogo Takashiba, Thomas E. Van Dyke, Salomon Amar, Yoji Murayama, Aubrey W. Soskolne, Lior Shapira

    Infection and Immunity   67 ( 11 )   5573 - 5578   1999年11月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    <title>ABSTRACT</title>
    During infection, circulating blood monocytes migrate from the vasculature to the extravascular compartments where they mature into tissue macrophages. The maturation process prepares the cell to actively participate in the inflammatory and the immune responses, and many transcription factors have been found to be involved. Here we report on a novel role for nuclear factor κB (NF-κB) in this process. Its accumulation in the cytoplasm of differentiated macrophages is responsible for the enhanced ability of the cell to respond to lipopolysaccharide (LPS) stimulation, as determined by tumor necrosis factor alpha (TNF-α) secretion. Differentiation of the human monocytic cell line THP-1 into macrophage-like cells was induced by exposure of the cells to phorbol myristate acetate. DNA-bindable NF-κB was not detected in the cytoplasm of undifferentiated THP-1 cells but accumulated in the cytoplasm of the cells following differentiation. No TNF-α was detected in the media of resting differentiated and nondifferentiated THP-1 cells. Stimulation with LPS of differentiated cells induced the production of higher levels of TNF-α than stimulation of nondifferentiated cells. This hyperresponsiveness to LPS was found in the mRNA and secreted TNF-α levels. Furthermore, stimulation with LPS induced the translocation of NF-κB from the cytoplasm into the nucleus. This translocation process was more rapid in the differentiated cells than in the nondifferentiated cells, and the resultant accumulated levels of NF-κB in the nucleus were higher. The DNA-bindable NF-κB was identified as a heterodimer of p65 and p50. The results suggest that NF-κB accumulation in the cytoplasm during maturation of monocytes to macrophages primes the cells for enhanced responsiveness to LPS and results in the rapid secretion of inflammatory mediators, such as TNF-α, by mature macrophages following LPS challenge.

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  • Identification by Subtractive Hybridization of a Novel Insertion Sequence Specific for Virulent Strains of Porphyromonas gingivalis 査読 国際誌

    Koichi Sawada, Susumu Kokeguchi, Hiroshi Hongyo, Satoko Sawada, Manabu Miyamoto, Hiroshi Maeda, Fusanori Nishimura, Shogo Takashiba, Yoji Murayama

    Infection and Immunity   67 ( 11 )   5621 - 5625   1999年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    <title>ABSTRACT</title>

    Subtractive hybridization was employed to isolate specific genes from virulent
    <italic>Porphyromonas gingivalis</italic>
    strains that are possibly related to abscess formation. The genomic DNA from the virulent strain
    <italic>P. gingivalis</italic>
    W83 was subtracted with DNA from the avirulent strain ATCC 33277. Three clones unique to strain W83 were isolated and sequenced. The cloned DNA fragments were 885, 369, and 132 bp and had slight homology with only
    <italic>Bacillus stearothermophilus</italic>
    IS
    <italic>5377</italic>
    , which is a putative transposase. The regions flanking the cloned DNA fragments were isolated and sequenced, and the gene structure around the clones was revealed. These three clones were located side-by-side in a gene reported as an outer membrane protein. The three clones interrupt the open reading frame of the outer membrane protein gene. This inserted DNA, consisting of three isolated clones, was designated IS
    <italic>1598</italic>
    , which was 1,396 bp (i.e., a 1,158-bp open reading frame) in length and was flanked by 16-bp terminal inverted repeats and a 9-bp duplicated target sequence. IS
    <italic>1598</italic>
    was detected in
    <italic>P. gingivalis</italic>
    W83, W50, and FDC 381 by Southern hybridization. All three
    <italic>P. gingivalis</italic>
    strains have been shown to possess abscess-forming ability in animal models. However, IS
    <italic>1598</italic>
    was not detected in avirulent strains of
    <italic>P. gingivalis</italic>
    , including ATCC 33277. The IS
    <italic>1598</italic>
    may interrupt the synthesis of the outer membrane protein, resulting in changes in the structure of the bacterial outer membrane. The IS
    <italic>1598</italic>
    isolated in this study is a novel insertion element which might be a specific marker for virulent
    <italic>P. gingivalis</italic>
    strains.

    DOI: 10.1128/iai.67.11.5621-5625.1999

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  • HLA genetics for diagnosis of susceptibility to early-onset periodontitis 査読 国際誌

    Shogo Takashiba, Hideki Ohyama, Kosuke Oyaizu, Nahoko Kogoe-Kato, Yoji Murayama

    Journal of Periodontal Research   34 ( 7 )   374 - 378   1999年10月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    Human leukocyte antigens (HLA) are essential in the recognition of foreign antigens in humoral immune response, which is genetically predetermined. Susceptibility to certain diseases that involve the immune response has been studied in relation to distinct HLA types. Although some diseases have been found Co correlate to specific HLA loci positively, it has been difficult to isolate HLA types that predispose patients to periodontal destruction. Here. we review the current knowledge and recent advances in HLA genetics and its biology, which determine susceptibility to early-onset periodontitis (EOP). The HLA-DRB1*1501-DQB1*0602 genotype has been found with increasing frequency in EOP patients. This HLA genotype expresses aspartic acid at position 57 and glycine at position 70 on the DQ beta chain, suggesting a capability to bind certain bacterial antigens. The T cell response against the outer membrane protein (Ag53) of Porphyromonas against gingivalis was examined via this HLA genotype. Strong T cell response against Ag53 p141-161 was inhibited partially by anti-DR antibody, but not by anti-DQ antibody, possible host and bacterial peptides capable of binding DRB1*1501 were elucidated when the peptide sequence was compared to gene and protein databases. These results suggest that patients who have the HLA-DRB1*1501-DQB1*0602 genotype may have an accelerated T cell response to certain periodontapathic bacteria such as P. gingivalis in hyperimmune reactions and thus increased susceptibility to EOP.

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  • Role of soluble interleukin-6 receptor in inflamed gingiva for binding of interleukin-6 to gingival fibroblasts 査読 国際誌

    Koji Naruishi, Shogo Takashiba, Hsin-Hua Chou, Hideo Arai, Fusanori Nishimura, Yoji Murayama

    Journal of Periodontal Research   34 ( 6 )   296 - 300   1999年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    Interleukin-6 (IL-6), frequently detected in periodontitis, is known to mediate important signals in the inflammatory cytokine network. Gingival fibroblasts (GF) secrete cytokines upon stimulation with inflammatory mediators. However, it is not clear if GF respond to IL-6. We examined the lL-6 receptor gene expression in GF. Furthermore, we tested whether GF are target cells for IL-6 by examination of binding of IL-6. GF were found to contain trace amounts of mRNA for IL-6 receptor (IL-GR), but had high levels of mRNA for 130-kDa glycoprotein (gp130), which is a signal transducer for IL-6/IL-6R complex. Based on this observation, we hypothesized that IL-6 could bind GF if exogenous soluble forms of IL-6R (sIL-6R) existed in the gingiva or culture condition. Thus, we investigated the existence of sIL-6R in gingiva using enzyme-linked immunosorbent assay and whether sIL-6R influenced the binding of IL-6 to GF in vitro. In inflamed gingiva, sIL-6R was detected and its concentration ranged from 150 to 700 pg/mu g protein. The sIL-6R enhanced the binding of IL-6 to GF in a dose-dependent manner. This enhancement was inhibited by an antibody against gp130, suggesting that the IL-6/sIL-6R complex bound to the fibroblasts via gp130. These data demonstrated that gingival fibroblasts can be target cells for IL-6 in the presence of appropriate amounts of sIL-6R. This situation may exist during inflammation in periodontal tissue.

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  • A novel lipopolysaccharide-induced transcription factor regulating tumor necrosis factor   gene expression: Molecular cloning, sequencing, characterization, and chromosomal assignment 査読

    F. Myokai, S. Takashiba, R. Lebo, S. Amar

    Proceedings of the National Academy of Sciences   96 ( 8 )   4518 - 4523   1999年4月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Proceedings of the National Academy of Sciences  

    DOI: 10.1073/pnas.96.8.4518

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  • In Vitro Induction of Activation-Induced Cell Death in Lymphocytes from Chronic Periodontal Lesions by Exogenous Fas Ligand 査読 国際誌

    Takamasa Sawa, Fusanori Nishimura, Hideki Ohyama, Keiso Takahashi, Shogo Takashiba, Yoji Murayama

    Infection and Immunity   67 ( 3 )   1450 - 1454   1999年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    <title>ABSTRACT</title>
    Periodontitis is a chronic inflammatory disease which gradually destroys the supporting tissues of the teeth, leading to tooth loss in adults. The lesions are characterized by a persistence of inflammatory cells in gingival and periodontal connective tissues. To understand what mechanisms are involved in the establishment of chronic lesions, we hypothesized that infiltrating lymphocytes might be resistant to apoptosis. However, both Bcl-2 and Bcl-xL were weakly detected in lymphocytes from the lesions, compared with those from peripheral blood, suggesting that these cells are susceptible to apoptosis. Nevertheless, very few apoptotic cells were observed in tissue sections from the lesions. Lymphocytes from the lesions expressed mRNA encoding Fas, whereas Fas-ligand mRNA was very weakly expressed in lymphocytes from the lesions and in periodontal tissues. Since the results indicated that lymphocytes in the lesions might be susceptible to Fas-mediated apoptosis but lack the death signal, we next investigated if these lymphocytes actually undergo apoptosis by the addition of anti-Fas antibodies in vitro. Fas-positive lymphocytes from the lesions underwent apoptosis by these antibodies, but Fas-negative lymphocytes and Fas-positive peripheral lymphocytes did not undergo apoptosis by these antibodies. These results indicate that lymphocytes in the lesions are susceptible to activation-induced cell death and are induced to die by apoptosis after the addition of exogenous Fas ligand.

    DOI: 10.1128/iai.67.3.1450-1454.1999

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  • Phylogenetic characterization of Centipeda periodontii, Selenomonas sputigena and Selenomonas species by 16S rRNA gene sequence analysis 査読 国際誌

    S Sawada, S Kokeguchi, F Nishimura, S Takashiba, Y Murayama

    MICROBIOS   98 ( 391 )   133 - 140   1999年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:FACULTY PRESS  

    The nearly complete 16S rRNA gene sequences for oral Gram-negative anaerobic motile bacteria, Centipeda periodontii, Selenomonas sputigena and Selenomonas species (formerly S. sputigena type strain), were determined in order to unveil their relationship to other oral motile bacteria. To determine the phylogenetic characterization of these bacteria, their 16S rRNA gene sequences were obtained and compared with those from the ribosomal sequence databases previously reported. The 16S rRNA gene sequences of these bacteria were similar to those of Selenomonas ruminantium and Schwartzia succinivorans isolated from rumens, and to Pectinatus cerevisiiphilus isolated from spoiled beer. Among oral bacteria, the nucleotide sequence analysis of these bacteria revealed high nucleotide similarity to Veillonella species, whereas low similarity to oral motile bacteria such as Campylobacter species. Phylogenetic analysis clearly confirmed that C. periodontii and two Selenomonas species were classified as relatives of a group besides Selenomonas, Schwartzia, and Pectinatus species, and not as close relatives to oral motile bacteria, such as Campylobacter species. These results suggest that such oral Gram-negative anaerobic motile bacteria are close relatives of oral bacteria.

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  • T cell responses to 53-kDa outer membrane protein of porphyromonas gingivalis in humans with early-onset periodontitis 査読 国際誌

    Hideki Ohyama, Sho Matsushita, Nahoko Kato, Fusanori Nishimura, Kosuke Oyaizu, Susumu Kokeguchi, Hidemi Kurihara, Shogo Takashiba, Yasuharu Nishimura, Yoji Murayama

    Human Immunology   59 ( 10 )   635 - 643   1998年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    Patients with early-onset periodontitis (EOP) are susceptible to infection with periodontopathic bacteria, such as Porphyromonas gingivalis. Ag53, 53-kDa outer membrane protein of P. gingivalis, evokes strong humoral immune responses in EOP patients. In a first step to clarify how host immune cells recognize Ag53, we established Ag53-specific short-term T cell lines from 22 subjects including 6 EOP patients and 16 healthy donors, using overlapping peptides based on Ag53 amino acid sequences. All T cell lines from active EOP patients recognized a common region (p141-181, especially p141-161) on Ag53, while those from healthy donors showed heterogeneous specificity. p141-181 was not recognized by T cell lines established from EOP patients following therapy. A monoclonal antibody to HSA-DRB1 inhibited Ag53-induced proliferation of most of the T cell lines. Our observations suggest that, although antigen-presenting molecules are common in EOP patients and in healthy individuals, p141-161 includes a major T cell epitope(s) on Ag53 for active EOP patients but not for healthy individuals or inactive EOP patients. (C) American Society for Histocompatibility and Immunogenetics, 1998 Published by Elsevier Science Inc.

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  • The S-layer protein fromCampylobacter rectus: sequence determination and function of the recombinant protein 査読 国際誌

    Manabu Miyamoto, Hiroshi Maeda, Michitaka Kitanaka, Susumu Kokeguchi, Shogo Takashiba, Yoji Murayama

    FEMS Microbiology Letters   166 ( 2 )   275 - 281   1998年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

    The gene encoding the crystalline surface layer (S-layer) protein from Campylobacter rectus, designated sip, was sequenced and the recombinant gene product was expressed in Escherichia coli. The gene consisted of 4086 nucleotides encoding a protein with 1361 amino acids. The N-terminal amino acid sequence revealed that Sip did not contain a signal sequence, but that the initial methionine residue was processed. The deduced amino acid sequence displayed some common characteristic features of S-layer proteins previously reported. A homology search showed a high similarity to the Campylobacter fetus S-layer proteins, especially in their N-terminus. The C-terminal third of Sip exhibited homology with the RTX toxins from Gram-negative bacteria via the region including the glycine-rich repeats. The Sip protein had the same N-terminal sequence as a 104-kDa cytotoxin isolated from the culture supernatants of C. rectus. However, neither native nor recombinant Sip showed cytotoxicity against HL-60 cells or human peripheral white blood cells. These data support the idea that the N-terminus acts as an anchor to the cell surface components and that the C-terminus is involved in the assembly and/or transport of the protein. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

    DOI: 10.1111/j.1574-6968.1998.tb13901.x

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  • Periodontal disease as a complication of diabetes mellitus. 査読 国際誌

    Nishimura F, Takahashi K, Kurihara M, Takashiba S, Murayama Y

    Annals of periodontology / the American Academy of Periodontology   3 ( 1 )   20 - 29   1998年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:1  

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  • Comparative study of two outer membrane protein genes from Porphyromonas gingivalis 査読 国際誌

    H Hongyo, S Kokeguchi, H Kurihara, M Miyamoto, H Maeda, S Takashiba, Y Murayama

    MICROBIOS   95 ( 381 )   91 - 100   1998年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:FACULTY PRESS  

    A periodontal pathogen, Porphyromonas gingivalis possesses either a 53 kD (Ag53) or a 67 kD (Ag67) outer membrane protein (OMP). Almost all sera from patients with periodontal diseases reacted strongly with either Ag53 or Ag67. In previous work the cloning and sequencing of the 53 kD outer membrane protein gene designated pga53 from Fl gingivalis FDC381, was reported and the presence of a gene homologous to pga53 in Fl gingivalis ATCC 33277 demonstrated. In the present work this pga53-homologous gene from Fl gingivalis ATCC 33277 was isolated and characterized. Nucleotide sequence analysis revealed that this gene encoded Ag67, and the gene was designated pga67. The deduced amino acid sequence and composition of pga67 was similar to the amino acid composition and N-terminal partial sequence of Ag67. An open reading frame of pga67 consisted of 1,692 nucleotides encoded as 564 amino acids, including a 49 amino acid signal sequence. The comparative analysis between pga67 and pga53 revealed that (1) the deduced amino acid sequence showed a 30.1% homology; (2) signal sequence and proline-rich regions at the C-terminus were the most conserved regions; (3) considerable differences were found mainly in the middle part of the OMPs; and (4) obvious differences in the two-dimensional models were evoked. These differences between pga67 and pga53 may explain the antigenic diversity between Ag67 and Ag53 OMPs.

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  • Host Defensive, Immunological, and Microbiological Observations of an Early-Onset Periodontitis Patient With Virus-Associated Hemophagocytic Syndrome 査読

    Takayuki Kono, Masayuki Takigawa, Fusanori Nishimura, Shogo Takashiba, Masatsugu Nakagawa, Hiroshi Maeda, Hideo Arai, Atsushi Nagai, Hidemi Kurihara, Yoji Murayama

    Journal of Periodontology   68 ( 12 )   1223 - 1230   1997年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    VIRUS-ASSOCIATED HEMOPHAGOCYTIC SYNDROME (VAHS) is a disorder characterized by benign generalized histiocytic proliferation and marked hemophagocytosis associated with systemic viral infection. An immunodeficiency which includes an extremely decreased leukocyte and platelet count together with abnormalities in the CD4/CD8 ratio are the most common features of VAHS. Here we report an early-onset periodontitis (EOP) patient with VAHS from the standpoint of host-parasite interaction to understand the effect of this systemic disorder which might possibly influence susceptibility to periodontal disease. The patient is a 16-year-old Japanese male clinically diagnosed as having generalized EOP with slight gingival inflammation and moderate bone loss. This patient manifested VAHS at 3 years of age, and then had an unusual 4 recurrences (at 5, 7, 11, and 14 years old). Laboratory tests conducted include: 1) complete blood analyses; 2) peripheral neutrophil functions (chemotaxis, phagocytosis, superoxide production, and adherence); 3) peripheral lymphocyte subpopulations and functions, T-cell proliferative activity and productivity of cytokines (interleukin-2 [IL-2], interferon gamma [IFN-gamma], and tumor necrosis factor alpha [TNF-alpha]); 4) serum cytokine levels cn. Ip, IL-2, soluble IL-2 receptor [sIL-2R], IL-4, IL-6, IFN-gamma, and TNF-alpha; 5) serum immunoglobulin G (IgG) antibody titers against periodontopathic bacteria; 6) serological human leukocyte antigen (HLA) typing; and 7) determination of bacterial flora of the periodontal pockets. The results indicated that the patient's neutrophil chemotaxis and random migration were below the normal range. In lymphocyte examinations, T-cell proliferative activity, IL-2, and IFN-gamma productivity were elevated. Serum IFN-gamma level was also significantly higher than normal range. No specific periodontopathic bacteria were predominant in the periodontal pockets, however, the serum IgG titer against Porphyromonas gingivalis was elevated throughout the examination period. It is suggested that VAHS might be a possible risk factor for periodontal disease, and hence may serve as a model in understanding the role of host defense mechanisms in the establishment of inflammatory periodontal disease.

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  • Comparison of in vitro proliferative capacity of human periodontal ligament cells in juvenile and aged donors 査読 国際誌

    F. Nishimura, V. P. Terranova, M. Braithwaite, R. Orman, H. Ohyama, J. Mineshiba, H. H. Chou, S. Takashiba, Y. Murayama

    Oral Diseases   3 ( 3 )   162 - 166   1997年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:3  

    OBJECTIVE: The aim of this study is to compare the in vitro proliferative capacity of periodontal ligament (PDL) cells from aged and juvenile donors. MATERIALS AND METHODS: Flow-cytometric analysis of the cell cycle was used to compare the length of each cell cycle, and the ratio of the cells progressing through the cycles between four PDL cells from juvenile donors and four cells from aged donors. Then, replicative capacity of the PDL cells from three juvenile and three aged donors was compared by serial cultures. Finally, expression of c-fos was compared between cells proliferating and cells which had reached senescent. RESULTS: Flow- cytometric analysis of the cell cycle had revealed that although there were no differences in the length of each phase of the cell cycle, significant differences were found in the ratio of the cells entering from Gap I to DNA synthesis phase of the cell cycle (P &lt
    0.025). Replicative capacity was much longer in two cells from juvenile donors (about 20 population doublings), while all cells from aged donors showed short dividing abilities (less than eight population doublings), hence entered senescent phases shortly. Additionally, no c-fos was detected in cells which had reached senescence upon stimulation with serum. CONCLUSIONS: It is generally believed that aged humans have an impaired wound healing ability. We believe that more fibrotic PDL tissues seen in aged humans might be the reason for this, and suggest that this phenomena might be due to the progressive accumulation of senescent cell populations.

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  • ヒト歯肉線維芽細胞のDNA合成および細胞外基質蛋白合成に及ぼすTNF-αの影響

    樋口 豊, 滝川 雅之, 新井 英雄, 鷲尾 憲文, 大江 丙午, 西村 英紀, 清水 秀樹, 野村 慶雄, 高柴 正悟, 村山 洋二

    日本歯周病学会会誌   39 ( 2 )   264 - 272   1997年6月

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    記述言語:日本語   出版者・発行元:特定非営利活動法人日本歯周病学会  

    腫瘍壊死因子(TNF)-αは,初期の炎症反応として産生される炎症性サイトカインのひとつで,炎症病巣の成立に関与していると考えられている。我々は,歯肉結合組織におけるTNF-αの役割を理解するために,ヒト歯肉線維芽細胞のDNA合成,コラーゲン合成,および非コラーゲン蛋白合成に対するTNF-αの作用を,線維芽細胞自らが産生するプロスタグランジン(PG)E_2,および血小板成長因子(PDGF)のオートクラインな影響を考慮して調べた。結果は以下の通りである。1) TNF-αはヒト歯肉線維芽細胞におけるDNA合成,コラーゲン合成,および非コラーゲン蛋白合成を促進した。2) TNF-αはヒト歯肉線維芽細胞におけるPGE_2産生を著明に促進した。3)インドメタシンを用いてPGE_2産生を阻害することにより,TNF-αがヒト歯肉線維芽細胞におけるDNA合成,コラーゲン合成,および非コラーゲン蛋白合成を促進する効果を増強した。4) TNF-αはヒト歯肉線維芽細胞におけるPDGF-A鎖mRNAの発現を促進した。以上の結果から,TNF-αはヒト歯肉線維芽細胞のDNA合成および細胞外基質蛋白合成に対し促進的に作用することが明らかとなった。また,これらの作用は,TNF-αが産生を誘導するPGE_2によって抑制を受けていた。さらにTNF-αがDNA合成を促進する作用には,内因性PDGFが関与している可能性が示唆された。

    DOI: 10.2329/perio.39.264

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  • Molecular cloning and characterization of the gene encoding 53 kD outer membrane protein of Porphyromonas gingivalis 査読 国際誌

    H Hongyo, H Kurihara, S Kokeguchi, M Miyamoto, H Maeda, M Hayakawa, Y Abiko, S Takashiba, Y Murayama

    MICROBIOS   92 ( 370 )   47 - 57   1997年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:FACULTY PRESS  

    The pga53 gene which encoded the antigenic 53 kD outer membrane protein (Ag53) was isolated from a genomic DNA library of Porphyromonas gingivalis FDC381 by using an Ag53-immunized rabbit serum. Determination of its complete nucleotide sequence revealed that the precursor of Ag53 had a 50 amino-acid putative signal sequence and the mature protein of 448 amino acids. The deduced amino acid sequence after a 50 amino-acid putative signal sequence was in complete agreement with the first 20 N-terminal amino acids of purified Ag53. Analysis of the deduced amino acid sequence revealed the presence of a highly hydrophilic proline-rich region at the C-terminal of Ag53. The deduced amino acid sequence showed 29.9% homology with that of a 72 kD cell-surface protein in P. gingivalis. Southern hybridization revealed that pga53 was specific to several P. gingivalis strains and that P. gingivalis strains which did not possess Ag53 had genes homologous to pga53.

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  • Inhibition of nuclear factor kappa B subunit p65 mRNA accumulation in lipopolysaccharide-stimulated human monocytic cells treated with sodium salicylate 査読

    S. Tokashiba, T. E. Dyke, S. Amar

    Oral Microbiology and Immunology   11 ( 6 )   420 - 424   1996年12月

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    担当区分:筆頭著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/j.1399-302x.1996.tb00205.x

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  • HLA Class II Genotypes Associated With Early-Onset Periodontitis: DQB1 Molecule Primarily Confers Susceptibility to the Disease 査読 国際誌

    Hideki Ohyama, Shogo Takashiba, Kosuke Oyaizu, Atsushi Nagai, Taeko Naruse, Hidetoshi Inoko, Hidemi Kurihara, Yoji Murayama

    Journal of Periodontology   67 ( 9 )   888 - 894   1996年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DNA TYPING WAS PERFORMED on 24 Japanese patients with early-onset periodontitis (EOP) using the PCR-RFLP method to investigate an association of the susceptibility to EOP with the particular HLA class II alleles (HLA-DRB1, -DQA1, and -DQB1), DRB1*1401, DRB1*1501, DQB1*0503, and DQB1*0602 were found more frequently (''susceptible'') in the EOP patients than in healthy controls. In contrast, DRB1*0405 and DQB1*0401 were found less frequently (''resistant'') in EOP patients, All patients carrying DQB1*0602 had an atypical BamHI site in the intron upstream of the third exon of the DQB1 gene, which in our previous studies appeared to be a susceptible marker for EOP. A comparative analysis of the amino acid sequences of these susceptible and resistant HLA-DRB1 and DQB1 alleles elucidated some differences in antigen-derived peptide binding sites related to the susceptible or resistant alleles. Especially, DQB1*0503 and DQB1*0602 alleles carrying aspartic acid at position 57 and glycine at position 70 are increased significantly in EOP. Since amino acid residues at positions 57 and 70 on the DQB1 molecule are supposed to be involved in antigen binding, amino acid substitutions at these positions may affect the immune responsiveness to the periodontopathic antigen. Our results suggest that the DQB1 molecule plays a crucial role in the pathogenesis of EOP and that the susceptibility to EOP may be determined by the binding ability between the peptide and HLA-DQ antigens.

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  • Host Defensive Functions in a Family Manifesting Early-Onset Periodontitis 査読 国際誌

    Hideo Arai, Toshihiro Chihara, Keiso Takahashi, Atsushi Nagai, Isao Akutsu, Shogo Takashiba, Fusanori Nishimura, Hidemi Kurihara, Yoji Murayama

    Journal of Periodontology   67 ( 4 )   433 - 442   1996年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    FAMILY CASE STUDIES HELP US IDENTIFY host risk factors in periodontal disease. In this study we examine a family consisting of a mother (40 years old, with rapidly progressive periodontitis), her elder daughter (14 years old, with localized juvenile periodontitis), and younger daughter (13 years old, with simple gingivitis). We examined 1) the peripheral neutrophil functions (chemotactic migration, phagocytosis, superoxide production); 2) lymphocyte functions (proliferative activity and cytokine productivity of T cells, immunoglobulin [Ig] M productivity of B cells when stimulated with pokeweed mitogen); 3) phenotypic analyses of peripheral lymphocyte subpopulations; 4) serum IgG antibody titers against periodontopathic bacteria; and 5) serological type of HLA class IS. All the subjects exhibited high T4/T8 ratios due to high percentage of CD4-positive cells, showed high IgG titers to Actinobacillus actinomycetemcomitans, and had a HLA DQw1 in common. The mother showed a slight deficiency of neutrophil chemotactic migration to N-formyl methyonyl leucyl phenylalanin (fMLP), raised interleukin-2 productivity of T cell, and high levels of IgG titers to Porphyromonus gingivalis and Fusobacterium nucleatum. Both daughters showed weak T cell proliferative response to anti-CD3 monoclonal antibody and low IgM productivity. Low lymphocyte responsiveness may be involved in the pathogenesis of periodontal disease of these daughters; therefore, the lymphocyte dysfunctions shown should be considered in relation to the progression of periodontal disease.

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  • Clinical and Laboratory Studies on a Patient With Early Onset Periodontitis and Her Family Members. A Case Report 査読

    Keiso Takahashi, Masayuki Takigawa, Hiroaki Hara, Atsushi Nagai, Shogo Takashiba, Fusanori Nishimura, Toshihiro Chibara, Hideki Ohyama, Nobuhiko Satoh, Hidemi Kurihara, Yoji Murayama

    Journal of Periodontology   66 ( 5 )   403 - 412   1995年5月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1902/jop.1995.66.5.403

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  • Lipopolysaccharide-inducible and salicylate-sensitive nuclear factor(s) on human tumor necrosis factor alpha promoter 査読 国際誌

    S Takashiba, T E Van Dyke, L Shapira, S Amar

    Infection and Immunity   63 ( 4 )   1529 - 1534   1995年4月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    Lipopolysaccharide (LPS) is one of the most potent trigger substances for monocytes and macrophages causing secretion of tumor necrosis factor alpha (TNF-alpha) and other inflammatory mediators. The nature of the nuclear factors involved in human TNF-alpha gene regulation is still unknown. Nuclear factor kappa B (NF-kappa B) proteins have been suggested to play an important role in gene transcription of inflammatory mediators when monocytes are stimulated with LPS. However, it remains unclear whether these nuclear factors are the only ones involved in human TNF-alpha gene regulation. In this report, to further the identification of nuclear factor(s) involved in TNF-alpha gene regulation, human monocytic THP-1 cells were transfected with a series of truncated versions of human TNF-alpha promoter. A 98-bp region located from nucleotides -584 to -487 demonstrated strong promoter activity. Electrophoretic mobility shift assays demonstrated that a 64-bp fragment located within the 98-bp region and lacking any potential NF-kappa B-binding sites avidly bound LPS-challenged THP-1 nuclear protein. Although this binding was inhibited in salicylate-treated cells, as was binding of NF-kappa B, the pattern of binding was found to differ from that noted for NF-kappa B. Analysis of this 64-bp fragment disclosed the absence of an NF-kappa B consensus sequence, suggesting a novel nuclear DNA-binding protein necessary for the initiation of human TNF-alpha transcription other than, or in addition to, NF-kappa B.

    DOI: 10.1128/iai.63.4.1529-1534.1995

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  • Prostaglandin E2 Inhibits Interleukin-6 Release But Not Its Transcription in Human Gingival Fibroblasts Stimulated With Interleukin-1β or Tumor Necrosis Factor-α 査読

    Masayuki Takigawa, Shogo Takashiba, Keiso Takahashi, Hideo Arai, Hidemi Kurihara, Yoji Murayama

    Journal of Periodontology   65 ( 12 )   1122 - 1127   1994年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    INFLAMMATORY MEDIATORS PRODUCED BY HUMAN GINGIVAL FIBROBLASTS (HGF) have been implicated in the initiation and progression of periodontal disease. The purpose of this study was to examine whether prostaglandin E(2) (PGE(2)), which is produced in abundance from HGF after stimulation with interleukin (IL)-1 beta or tumor necrosis factor-alpha (TNF-alpha), could regulate IL-6 production by HGF. HGF stimulated with either IL-1 beta or TNF-alpha showed a rapid and dose-dependent increase in IL-6 mRNA accumulation and IL-6 secretion, as demonstrated by reverse transcription-polymerase chain reaction analysis and bioassay. IL-6 secretion from either IL-1 beta- or TNF-alpha-stimulated HGF was enhanced by the inhibition of PGE(2) synthesis with indomethacin. Furthermore, the addition of PGE(2) inhibited IL-6 secretion from these cells. In contrast, indomethacin or PGE(2) did not affect the accumulation of IL-6 mRNA in IL-1 beta-stimulated HGF. These data indicate that IL-6 production by HGF is up-regulated by specific cytokines, IL-1 beta and TNF-alpha, and suggest that this production may be partially down-regulated by endogenous and exogenous PGE(2) at the post-transcriptional level.

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  • Cytokine-Dependent Synergistic Regulation of Interleukin-8 Production From Human Gingival Fibroblasts 査読 国際誌

    Masayuki Takigawa, Shogo Takashiba, Fumio Myokai, Keiso Takahashi, Hideo Arai, Hidemi Kurihara, Yoji Murayama

    Journal of Periodontology   65 ( 11 )   1002 - 1007   1994年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    HUMAN GINGIVAL FIBROBLASTS (HGF) may have an important role in the orchestration of immuno participant cells infiltrating the gingiva in response to continuously recurring bacterial infection. To examine the cytokine network regulating HGF-derived interleukin (IL)-8, a potent neutrophil chemotactic cytokine, we analyzed the effects of inflammatory cytokines alone and iii combination on IL-8 production by HGF. IL-1 beta, tumor necrosis factor-alpha (TNF-alpha), Interferon-gamma (IFN-gamma), IL-6, and IL-8 were used as stimulants. HGF secreted IL-8 in a dose-dependent manner after stimulation with either IL-1 beta or TNF-alpha, but not with IFN-gamma or IL-6. Furthermore, IL-8 itself did not affect IL-8 mRNA accumulation in HGF in an autocrine manner, The combination of IL-1 beta and TNF-alpha synergistically enhanced the secretion of IL-8, whereas IFN-gamma suppressed IL-8 secretion by IL-1 beta- or TNF-alpha-stimulated HGF. These effects were also observed at each level of IL-8 mRNA expression in HGF. IL-8 secretion by cytokine-stimulated HGF was not influenced my the inhibition of PGE(2) synthesis with indomethacin, indicating that endogenous PGE(2) was not involved in IL-8 production by HGF. These results indicate that IL-8 production by HGF is synergistically stimulated by specific cytokines, IL-1 beta and TNF-alpha, and suggest that these stimulatory effects are down-regulated by IFN-gamma at the transcriptional let ei through PGE(2)-independent pathways. Thus, neutrophil-mediated processes in periodontal disease may be regulated in part by HGF in the cytokine network of immuno-participant cells.

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  • Molecular Basis of Leukocyte Adhesion Molecules in Early-Onset Periodontitis Patients With Decreased CD11/CD18 Expression on Leukocytes 査読 国際誌

    Kyoko Katsuragi, Shogo Takashiba, Hidemi Kurihara, Yoji Murayama

    Journal of Periodontology   65 ( 10 )   949 - 957   1994年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    WE ANALYZED THE CELL-CELL ADHERENCE RELATED to CD11/CD18 and CD18 mRNA in individuals with decreased CD11/CD18 expression on their neutrophil surface. Epstein Barr virus-transformed B cell lines were developed from one localized juvenile periodontitis (LJP) patient with decreased CD11/CD18 in the peripheral blood neutrophils and without systemic diseases; two siblings with generalized prepubertal periodontitis (GPP) caused by leukocyte adhesion deficiency (LAD); another LJP patient; one localized prepubertal periodontitis (LPP) patient; and two healthy subjects. Adhesion of leukocytes to each other was measured as cluster formation by aggregation assay. The length and the amount of CD18 mRNA expressed in the cell lines were analyzed by Northern blotting using the P-32-labeled CD18 cDNA. The coding region of the mRNA was analyzed by the reverse transcription-polymerase chain reaction method. Base-mismatches between CD18 mRNA and the P-32-labeled RNA probe synthesized from CD18 cDNA were analyzed by RNase protection assay. In the adherence assay, cells from the LJP patients with decreased CD11/CD18 formed more clusters of smaller size and fewer cells than those of the other subjects. The cells from GPP and LAD patients did not aggregate and did not form clusters either in the absence or presence of PMA. There were no differences in the length and the amount of mRNA between the LJP patients and the other subjects, while GPP-LAD patients expressed a small amount of long mRNA. The whole coding region (2,313 base pairs) of all subjects was amplified except for the GPP-LAD patients, and the 5'-region (1,119 base pairs) was amplified from all subjects. Base-mismatches were detected on the coding region from 965 to 1,450 nucleotides of CD18 mRNA in GPP-LAD patients. No mismatch was detected on other regions of CD18 mRNA in any subject. These results suggest that the LJP patient with an anomaly of qualitative aggregation related to CD11/CD18 is not related to sequence abnormalities of CD18 mRNA suggesting other mechanisms. In contrast, the adherence defect in two GPP-LAD patients was related to heterogeneous molecular mutations on CD18.

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  • An Atypical Site In HLA-DQb1 Detected in Leprosy Patients 査読

    H OHYAMA, A NAGAI, S TAKASHIBA, K SUGIYAMA, S INOUE, M MIZUSHIMA, A KOHZUMA, Y MURAYAMA

    INTERNATIONAL JOURNAL OF LEPROSY AND OTHER MYCOBACTERIAL DISEASES   62 ( 2 )   293 - 294   1994年6月

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    記述言語:英語   出版者・発行元:AMER LEPROSY MISSION  

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  • Unique Intronic Variations of HLA-DQβ Gene in Early-Onset Periodontitis 査読 国際誌

    Shogo Takashiba, Sumihare Noji, Fusanori Nishimura, Hideki Ohyama, Hidemi Kurihara, Yoshio Nomura, Shigehiko Taniguchi, Yoji Murayama

    Journal of Periodontology   65 ( 5 )   379 - 386   1994年5月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    HUMAN LEUKOCYTE ANTIGEN (HLA) class II beta chain plays an important role in the recognition of foreign antigens in immune reactions. Different forms of immune reaction may be concerned with initiation and progression of infectious diseases such as periodontitis. In this study we examined the frequency of HLA class II serotype and the variation of HLA class II beta gene in periodontitis patients. HLA serotypic frequencies in 70 Japanese patients with periodontitis and 26 individuals with periodontal health were examined. No HLA serotype specific to any type of periodontitis was observed. In order to detect differences among some HLA serotypes, restriction fragment length polymorphism (RFLP) analysis was undertaken with cDNA probes for HLA-DR beta and HLA-DQ beta genes in 20 subjects (15 patients and 5 healthy individuals). Atypical BamHI and EcoRI restriction sites were found in the HLA-DQ beta gene from 3 patients with early-onset periodontitis. In addition to these 20 subjects, an additional 80 subjects (40 patients and 40 healthy individuals) were screened for the atypical BamHI restriction site using the polymerase chain reaction method. It was detected in 7 patients with early-onset periodontitis, 1 patient with adult periodontitis, and 3 healthy subjects. No clinical differences except age were found between patients with this gene variation and other patients. Interestingly, all 3 healthy subjects with this gene variation were from subjects whose family members developed early-onset periodontitis with the gene variation. Atypical BamHI and EcoRI restriction sites and 41-nt repeated sequence were found in the intron before the third exon of HLA-DQB gene. These results suggest that these intronic gene variations may be useful as gene markers for a subpopulation of early-onset periodontitis and might affect immune reactions such as antigen recognition.

    DOI: 10.1902/jop.1994.65.5.379

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  • Porphyromonas gingivalis lipopolysaccharide stimulation of human monocytes: dependence on serum and CD14 receptor 査読 国際誌

    L. Shapira, S. Takashiba, S. Amar, T. E. Dyke

    Oral Microbiology and Immunology   9 ( 2 )   112 - 117   1994年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    The purpose of this study was to investigate factors influencing the ability of lipopolysaccharide (LPS) derived from Porphyromonas gingivalis to elicit secretion of tumor necrosis factor-alpha (TNFalpha) from human monocytes (adherent mononuclear cells). The results indicate that P gingivalis LPS stimulation of TNFalpha from monocytes is comparable to LPS from Escherichia coli. Both LPS, although structurally different, increased TNFalpha secretion in a dose-dependent manner. In serum-free conditions, TNFalpha secretion was relatively low, but it dramatically increased at human serum concentrations as low as 1%. Maximal secretion was observed in the presence of 10% serum, with a slight decrease at higher serum concentrations. The CD14 molecule is a putative monocyte LPS receptor. When cells were pre-incubated with a blocking monoclonal antibody (My4) to CD14, TNFalpha-mRNA accumulation and TNFalpha secretion were reduced to control levels at LPS concentrations of up to 10 ng/ml. At higher LPS concentrations, the blocking effect was only partial, in spite of 50-fold excess antibody concentration. The blocking effect was observed only in the presence of serum. The effect of the CD14 antibody was dose-dependent with saturation at 2.5 mug/ml. The results suggest that CD14 is one of the major receptors for P gingivalis LPS but highlight the necessity to investigate other cell-surface receptors mediating P gingivalis-LPS interactions. These interactions are believed to be important in the pathogenesis of periodontal destruction.

    DOI: 10.1111/j.1399-302x.1994.tb00044.x

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  • Role of Cytokine in the Induction of Adhesion Molecules on Cultured Human Gingival Fibroblasts 査読 国際誌

    Keiso Takahashi, Masayuki Takigawa, Shogo Takashiba, Atsushi Nagai, Manabu Miyamoto, Hidemi Kurihara, Yoji Murayama

    Journal of Periodontology   65 ( 3 )   230 - 235   1994年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    THIS STUDY WAS UNDERTAKEN IN AN EFFORT to understand the role of cytokines on human gingival fibroblasts and T lymphocyte trafficing into inflamed gingival tissue. Using flow cytometry we examined gingival fibroblasts to determine the level of cell surface expression and the percentage of cells positive for intercellular adhesion molecule 1 (ICAM-1), the HLA-DR antigen, lymphocyte function-associated antigen 3 (LFA-3), and the CD44 molecule, which are involved in antigen presentation. The following cytokines were used: interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), IL-6, and IL-8. The levels of ICAM-1 expression were enhanced in a dose- and time-dependent manner by IL-1 beta, TNF-alpha, or IFN-gamma, but not by IL-6 or IL-8. HLA-DR surface expression was induced only by IFN-gamma in a dose- and time-dependent manner, but not by the other cytokines tested. In contrast, the expression of LFA3 and the CD44 molecule could be detected without the stimulation of any cytokine, but the levels of their expression were not significantly changed by any cytokines. The enhanced ICAM-1 expression by cytokines was reduced in a time-dependent manner following the removal of cytokines from the reaction mixture, while IFN-gamma-induced HLA-DR expression was maintained even 7 days after the removal of IFN-gamma. These data support an interactive role for inflammatory cytokines and the expression of adhesion molecules on gingival fibroblasts in the pathogenesis of gingival inflammation in periodontal disease.

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  • Assessment of Interleukin-6 in the Pathogenesis of Periodontal Disease 査読 国際誌

    Keiso Takahashi, Shogo Takashiba, Atsushi Nagai, Masayuki Takigawa, Fumio Myoukai, Hidemi Kurihara, Yoji Murayama

    Journal of Periodontology   65 ( 2 )   147 - 153   1994年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    THIS STUDY WAS PERFORMED TO INVESTIGATE the aspects of interleukin-6 (IL-6) production in both the gingival tissue and the peripheral blood of patients with periodontal disease and of periodontally healthy subjects. In addition, IL-6 expression in human gingival tissues was studied by reverse transcription-polymerase chain reaction analysis and by immunoperoxidase staining with anti-IL-6 monoclonal antibody. The levels of IL-6 in the culture supernatants from peripheral blood mononuclear cells (PBMC) stimulated with lipopolysaccharide and in serum were examined by bioassay. We detected IL-6 mRNA expression in all inflamed gingival tissues (17/17) examined and in 2/4 in healthy gingival tissues. IL-6 protein was detected mainly in endothelial cells, fibroblasts, and macrophages but not in the area containing T or B cells in the inflamed gingival tissues, and was not detected at all in healthy gingival tissues. There was no significant difference between the subjects with periodontal disease and those with healthy gingival tissues either in serum IL-6 levels or in the amount of IL-6 produced by PBMC. These results suggest that non-lymphoid cells in inflamed gingival tissue may contribute to the pathogenesis of periodontal disease via IL-6 production, and that the IL-6 produced in gingival tissue may not reflect the IL-6 levels in peripheral blood. 3.

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  • LEUKOCYTE ADHESION MOLECULES CD11/CD18 AND THEIR ROLE IN PERIODONTAL-DISEASES 査読

    Y MURAYAMA, K KATSURAGI, S TAKASHIBA, H KURIHARA

    MOLECULAR PATHOGENESIS OF PERIODONTAL DISEASE   215 - 233   1994年

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:AMER SOC MICROBIOLOGY  

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  • Cloning and characterization of human TNFα promoter region 査読 国際誌

    Shogo Takashiba, Lior Shapira, Salomon Amar, Thomas E. Van Dyke

    Gene   131 ( 2 )   307 - 308   1993年9月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    We report the sequence of a 1.2-kb human tumor necrosis factor alpha (TNFalpha) promoter region, which was cloned using PCR. The sequence has several variations from two previous reports and exhibits many potential DNA-binding sites specific to mammalian gene regulatory proteins inducible by lipopolysaccharides.

    DOI: 10.1016/0378-1119(93)90314-s

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  • Rapid fluorometric quantificaition of monocyte attachment in tissue culture wells 査読 国際誌

    Lior Shapira, Shogo Takashiba, John R. Kalmar, Thomas E. Van Dyke, Vivian Barak, W. Aubrey Soskolne

    Journal of Immunological Methods   165 ( 1 )   93 - 98   1993年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    A simple fluorometric assay that permits rapid quantification of attachment of monocytes or macrophages in tissue culture wells is described. Using 4,6-diamidino-2-phenylindole (DAPI) as a specific fluorochrome marker for DNA, we observed a dose-dependent increase with strong linear correlation in fluorescent emission over a broad range of DNA concentrations. Measurements of the DNA content of the human monocytic cell line THP-1 demonstrated a linear correlation between fluorescence intensity and cell number from 5 x 10(4) to 1 x 10(6) cells, with an estimated average DNA content of 7.5 pg DNA per cell. While untreated THP-1 cells were not detectably adherent, PMA induction for 24 h results in 57-76% adherence to plastic surface. This method was found to be useful for measuring the number of peripheral blood monocytes separated from lymphocytes by attachment. 16 subjects were sampled and the standard deviation of each individual did not exceed 10%. The number of attached cells was between 10-16% of the total mononuclear cells. Fluorescence measurement of DNA with DAPI permits rapid and accurate determination of cell numbers and appears useful in the quantification of adherent populations such as myelocytic cells and cell lines.

    DOI: 10.1016/0022-1759(93)90110-s

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  • Interleukin-8 is a major neutrophil chemotactic factor derived from cultured human gingival fibroblasts stimulated with interleukin-1 beta or tumor necrosis factor alpha 査読 国際誌

    S Takashiba, M Takigawa, K Takahashi, F Myokai, F Nishimura, T Chihara, H Kurihara, Y Nomura, Y Murayama

    Infection and Immunity   60 ( 12 )   5253 - 5258   1992年12月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    Inflammatory mediators produced by cells in the gingiva have been implicated in the initiation and progression of periodontal disease, a common infectious disease. In this study, we examined the biological activity of neutrophil chemotactic factors and the kinetics of expression of interleukin-8 (IL-8) mRNA derived from normal gingival fibroblasts in response to inflammatory mediators in an in vitro model. Gingival fibroblasts stimulated by either recombinant human interleukin-1 beta or recombinant human tumor necrosis factor alpha produced neutrophil chemotactic factors after 4 h, whereas expression of cell-derived IL-8 mRNA was detected within 1 h after stimulation. Furthermore, in a neutralization assay, rabbit anti-recombinant human IL-8 antiserum inhibited neutrophil chemotactic activity to basal levels. These results provide evidence that gingival fibroblasts synthesize potent chemotactic factors such as IL-8 in the presence of the inflammatory mediators interleukin-1 beta and tumor necrosis factor alpha. The activity of these factors may contribute to neutrophil-mediated processes in the pathogenesis of periodontal disease.

    DOI: 10.1128/iai.60.12.5253-5258.1992

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  • 早期発症型歯周炎を発現する1家族の宿主細胞機能所見

    千原 敏裕, 永井 淳, 阿久津 功, 本行 博, 高橋 慶壮, 清水 尚子, 谷本 一郎, 島袋 修, 藤田 直子, 宮本 学, 高柴 正悟, 後藤 弘幸, 西村 英紀, 磯島 修, 清水 秀樹, 栗原 英見, 野村 慶雄, 村山 洋二

    日本歯周病学会会誌   34 ( 1 )   204 - 212   1992年3月

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    記述言語:日本語   出版者・発行元:特定非営利活動法人日本歯周病学会  

    早期発症型歯周炎を発症している家族(母親とその娘2人)の歯周病病態を宿主防御細胞機能に重点を置いて解析した。母親(40歳)は急速進行性歯周炎,娘A (14歳)は限局性若年性歯周炎,そして娘B (13歳)は単純性歯肉炎と臨床診断した。好中球機能は,母親が遊走能において低かった。CD4陽性細胞検出率およびT4/T8は母娘全員が高い値を示した。CD3抗体で刺激したときのTリンパ球増殖活性は,娘Bが低かった。HLAフェノタイプは,母娘で共通してDQw1とw3およびDRw10とw12を検出した。Actinobacillus actinomycetemcomitansに対し母親,娘Aおよび娘Bが,Porphyromonas gingivalisに対し母親と娘Bが,Fusobacterium nucleatumに対し母親が高いIgG抗体価を示した。本家族の歯周病発症機序は,本研究において調べた生体防御機能の諸機能所見だけから,明確にできるものではなかった。歯周病の病態解析には,さらに幅広い生体防御機構のネットワークを念頭に置く必要がある。

    DOI: 10.2329/perio.34.204

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  • 抗体産生関連リンパ球諸機能の歯周病病態への関わりの研究

    高橋 慶壮, 永井 淳, 阿久津 功, 佐藤 暢彦, 高柴 正悟, 宮本 学, 滝川 雅之, 明貝 文夫, 葛城 教子, 橋本 俊明, 野村 慶雄, 村山 洋二

    日本歯周病学会会誌   33 ( 3 )   685 - 694   1991年9月

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    記述言語:日本語   出版者・発行元:特定非営利活動法人日本歯周病学会  

    歯周病患者の抗体産生に関連する免疫ネットワーク諸因子間の関わりを知ることを目的に,歯周病原性細菌に対して"低い血清抗体価"を示す歯周病患者18名と"高い血清抗体価"を示す患者14名,および健常者18名について,末梢血レベルにおける抗体産生能,単核球サブセットの割合,およびT細胞と単球の機能を調べた。健常者群と比較して,患者群はサプレッサーT細胞数の低下と抗体産生能の亢進を示す特徴を有していた。また,調べた諸機能の亢進あるいは低下を示す被験者の割合は血清抗体価の高低に関わらず患者群に多かった。しかし,調べた諸機能値はいずれも個体差によるばらつきが大きかった。体液性免疫応答に関連する個体のリンパ球因子間の関わりは,個体毎に明らかにされなければならないと考えられる。

    DOI: 10.2329/perio.33.685

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  • A Family Study of a Mother and Daughter with Increased Susceptibility to Early-Onset Periodontitis: Microbiological, Immunological, Host Defensive, and Genetic Analyses 査読 国際誌

    Fusanori Nishimura, Atsushi Nagai, Keiji Kurimoto, Osama Isoshima, Shogo Takashiba, Mitsuharu Kobayashi, Isao Akutsu, Hidemi Kurihara, Yoshio Nomura, Yoji Murayama, Hiroyuki Ohta, Keijiro Kato

    Journal of Periodontology   61 ( 12 )   755 - 765   1990年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    Microbiological, immunological, host-defensive, and genetic analyses were performed on a mother and daughter, both of whom had early-onset periodontitis (rapidly progressive periodontitis in the mother; localized juvenile periodontitis in the daughter). Microscopic examination revealed a greatly elevated percentage of rod-form bacteria in both subjects. Fusobacterium sp. and Porphyromonas gingivalis (formerly Bacteroides gingivalis) were the predominant microorganisms cultured. The humoral immune responses to F. nucleatum, P. gingivalis, and Actinobacillus actinomycetemcomitans were much higher in both subjects than those to any other periodontal bacteria examined. Functional and phenotypic analysis of the peripheral lymphocytes showed no significant abnormalities. However, investigation of neutrophil function showed that the mother had depressed neutrophil chemotaxis and superoxide production. The daughter had depression not only of chemotaxis and superoxide production, but also of neutrophil phagocytosis. Serological typing of HLA antigens revealed the same Class II HLA profile in both subjects. It was concluded that both subjects very probably had an identical condition and that these patients provided a unique model for improving our understanding of the host factors involved in periodontal disease.

    DOI: 10.1902/jop.1990.61.12.755

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  • ミノサイクリンの局所的応用による歯周炎治療法 : LS-007による治療方法の臨床的検討

    栗本 桂二, 磯島 修, 直良 有香, 穴田 高, 小林 芳友, 小林 充治, 新井 英雄, 高柴 正悟, 難波 秀樹, 横山 雅之, 光田 由可, 水島 ゆみ, 野村 慶雄, 村山 洋二, 上田 雅俊, 寺西 義浩, 藤原 一幸, 橋爪 彰子, 釜谷 晋平, 細山 陽子, 上羽 建二, 大西 和久, 白井 健雄, 大橋 哲, 東 浩介, 木岡 慶文, 南林 繁良, 田中 真弓, 北村 卓也, 牧草 一人, 山岡 昭, 浦口 良治, 萩原 さつき, 福田 光男, 小田 茂, 林 成忠, 竹蓋 弥, 米良 豊常, 峯岸 大造, 梅田 誠, 中元 弘, 稲富 洋文, ナロンサック ラーシイシン, 野口 俊英, 石川 烈

    日本歯周病学会会誌   30 ( 1 )   191 - 205   1988年3月

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    記述言語:日本語   出版者・発行元:特定非営利活動法人日本歯周病学会  

    塩酸ミノサイクリン(MINO,日本レダリー,東京)を用いて歯周炎の局所治療法を確立するための研究を行なってきた。本研究は,MINOを2% (力価)に含有する軟膏製剤(LS-007)を臨床的に用い,その有効性,安全性ならびに有用性をもとに用法を検討したものである。4mm以上のポケットを有する辺縁性歯周炎患者45名の119歯を被験歯とし,LS-007とそのプラセボ,および市販のミノマイシン錠(日本レダリー)を用い,微生物学的および臨床的に用法を検討した。その結果,LS-007の局所投与は歯周病治療において,臨床的有効性,安全性および有用性があると結論した。

    DOI: 10.2329/perio.30.191

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  • POLYMORPHISM OF COMPROMISED HOSTS IN PERIODONTITIS PATIENTS 査読

    S TAKASHIBA, F NISHIMURA, M KOBAYASHI, A NAGAI, AKUTSU, I, K OKAMURA, O ISOSHIMA, H KURIHARA, Y NOMURA, Y MURAYAMA

    RECENT ADVANCES IN CLINICAL PERIODONTOLOGY   790   383 - 386   1988年

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:ELSEVIER SCIENCE PUBL B V  

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  • 早期発症型歯周炎を伴った母娘症例の細菌叢,ならびに宿主防御機能に関する研究

    西村 英紀, 永井 淳, 岡村 和則, 栗本 桂二, 磯島 修, 直良 有香, 熊澤 寛, 杉山 雅昭, 穴田 高, 清水 秀樹, 中村 哲也, 小林 芳友, 小林 充治, 高柴 正悟, 水島 ゆみ, 光田 由可, 横山 雅之, 栗原 英見, 木下 正彦, 野村 慶雄, 村山 洋二, 太田 寛行, 加藤 慶二郎

    日本歯周病学会会誌   29 ( 2 )   492 - 505   1987年6月

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    記述言語:日本語   出版者・発行元:特定非営利活動法人日本歯周病学会  

    早期発症型歯周炎は宿主-寄生体相互作用の概念から注目される。家族性に発症した早期発症型歯周炎の1症例に対し, 紬菌学的ならぴに宿主防御機能の面から検索を行った。38歳の母親は, 重篤な歯肉の炎症症状と進行した骨破壊を呈し, 重度進行性歯周炎と診断した。16歳の娘は, 歯肉の炎症はないが, 局所的骨破壊が進行し, 限局型若年性歯周炎と診断した。母のポケットでは, 桿状菌とスピロへ一タが高率を占めた。娘のポケット内も, 桿状菌の占有率は高いが, スピロヘータは少なかった。母娘ともに優勢菌は Fusobacterium 種であった。A. actinomycetemcomitans は検出されなかった。体液性免疫応答は, 母娘ともに A. actinomycetemcomitans, B. gingivalis, F. nulcleatum に対して高い IgG 抗体反応で示された。好中球機能は, 母は貪食能において, 娘は貪食能と遊走能において低下があった。母娘の病態は酷似しており, 発症のメカニズムが同じであると推測される。

    DOI: 10.2329/perio.29.492

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  • 歯周病関連細菌に対する血清IgG抗体 : 第2報 歯周病治療に伴う血清IgG抗体の変動

    岡村 和則, 永井 淳, 熊澤 寛, 杉山 雅昭, 水島 ゆみ, 光田 由可, 高柴 正悟, 栗原 英見, 野村 慶雄, 村山 洋二

    日本歯周病学会会誌   29 ( 1 )   146 - 154   1987年3月

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    記述言語:日本語   出版者・発行元:特定非営利活動法人日本歯周病学会  

    歯周病患者71人を対象として, 歯周病関連細菌に対する血清IgG抗体が, 歯周病治療に伴いどのように変動するかを調べた。歯周病関連細菌として, Bacteroides gingivalis, Actinobacilus actinomycetemcomitans, Capnocytophaga ochraceaを取り上げた。抗体の測定には, 酵素免疫測定法 (ELlSA) を用いた。処置に伴い, 各菌種に対する抗体価は下降した。なかでも, 治療が終了したものでは有意な下降が示された。また, 観血的歯周病治療後の経過日数により, 抗体価の変動の様相に違いがあった。30日未満では, 変化がないかむしろ上昇傾向が認められ, 30日以降には有意な下降が認められた。以上のことから, 歯周病関連細菌に対する血清IgG抗体は, 歯周病治療に伴い変動し, 治療効果の判定に有効な指標となりうることが示唆された。

    DOI: 10.2329/perio.29.146

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    その他リンク: http://search.jamas.or.jp/link/ui/1988088820

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書籍等出版物

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    (NPO) 日本歯周病学会  2016年 

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  • 歯周病評価における最適検査部位の選定 項目反応理論Graded response modelの応用

    両角 俊哉, 野村 義明, 福田 光男, 花田 信弘, 角田 衣理加, 小林 宏明, 三邉 正人, 中村 利明, 中山 洋平, 西村 英紀, 野口 和行, 沼部 幸博, 小方 頼昌, 齋藤 淳, 佐藤 聡, 関野 愉, 菅野 直之, 菅谷 勉, 鈴木 史彦, 多部田 康一, 高橋 慶壮, 高井 英樹, 高柴 正悟, 梅田 誠, 吉江 弘正, 吉村 篤利, 吉成 伸夫, 中川 種昭

    日本歯周病学会会誌   63 ( 春季特別 )   106 - 106   2021年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 不妊治療中患者に対する血清IgG抗体価検査を用いた歯周病原細菌の感染度調査

    亀井 千晶, 大森 一弘, 佐光 秀文, 坂井田 京佑, 徳善 真砂子, 平井 公人, 小林 寛也, 山本 直史, 滝川 雅之, 三宅 貴仁, 高柴 正悟

    日本歯周病学会会誌   63 ( 春季特別 )   102 - 102   2021年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 歯科保存治療での「保存の可否」とは? 生命を紡ぐ

    高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   154回   40 - 40   2021年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

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  • Aggregatibacter actinomycetemcomitansに対し高い血清IgG抗体価反応を示す歯周炎患者の治療経過と病態考察

    岡本 憲太郎, 高知 信介, 小林 寛也, 大森 一弘, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   63 ( 春季特別 )   120 - 120   2021年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • ω-3脂肪酸誘導体の抗炎症作用による歯髄保存の試み

    米田 光宏, Zulema Rosalia Arias Martinez, 中村 心, 岡本 憲太郎, 伊東 昌洋, 田村 和也, 井手口 英隆, 大森 一弘, 山城 圭介, 山本 直史, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   154回   140 - 140   2021年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

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  • X連鎖性低リン血症性くる病を起因とした多発根尖性歯周炎に対し歯内療法を行った症例の病態考察

    佐光 秀文, 大森 一弘, 坂井田 京佑, 亀井 千晶, 小林 寛也, 井手口 英隆, 山本 直史, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   154回   126 - 126   2021年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

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  • 歯科保存治療での「保存の可否」とは? 生命を紡ぐ

    高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   154回   40 - 40   2021年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

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  • 大豆発酵食品テンペに含まれる抗菌性物質の単離と同定

    伊東 昌洋, 伊東 孝, 中村 心, 青木 秀之, 西岡 功志, 塩川 つぐみ, 多田 宏子, 竹内 祐貴, 武安 伸幸, 山本 直史, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   154回   159 - 159   2021年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

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  • HMGB1はM1マクロファージの分化を制御して歯周炎の進行に影響を及ぼす

    平井 杏奈, 井手口 英隆, 山城 圭介, Yao Zhang, 青柳 浩明, 山本 直史, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   154回   155 - 155   2021年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

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  • 【産婦人科医も知っておきたい歯科の知識】歯周病と不妊

    大森 一弘, 高柴 正悟, 三宅 貴仁

    産科と婦人科   88 ( 4 )   465 - 469   2021年4月

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    記述言語:日本語   出版者・発行元:(株)診断と治療社  

    歯周病は、Porphyromonas gingivalisに代表される歯周病原細菌が歯周組織に感染して発症する口腔感染症である。近年、歯周病原細菌の感染、そして、感染によって惹起された歯周炎症が不妊環境の構築に関与する可能性が示唆されはじめている。本稿では、歯周病と不妊の関連について文献的に考察するとともに、不妊治療中の重度歯周病患者に専門的治療を行うことによって自然妊娠・正常出産に至った実症例を提示しながら、その関連性を考察する。(著者抄録)

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    その他リンク: https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2021&ichushi_jid=J00525&link_issn=&doc_id=20210324150010&doc_link_id=%2Fae4sanke%2F2021%2F008804%2F011%2F0465b0469%26dl%3D3&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fae4sanke%2F2021%2F008804%2F011%2F0465b0469%26dl%3D3&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_4.gif

  • コラーゲン結合型塩基性線維芽細胞増殖因子を用いた水平性骨吸収に対する歯周組織再生療法の開発

    中村 心, 伊東 孝, 岡本 憲太郎, 美間 健彦, 内田 健太郎, 山本 直史, 松下 治, 高柴 正悟

    日本歯科医学会誌   40   78 - 78   2021年3月

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    記述言語:日本語   出版者・発行元:日本歯科医学会  

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  • 臨床推論の教育をどうするか 病態の理解は検査と臨床推論から

    高柴 正悟

    日本口腔診断学会雑誌   34 ( 1 )   52 - 52   2021年2月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔診断学会  

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  • 歯内療法の薬物的な新規治療

    高柴 正悟

    日本歯内療法学会雑誌   42 ( 1 )   1 - 4   2021年1月

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    記述言語:日本語   出版者・発行元:日本歯内療法学会  

    炎症の場においては、組織の炎症を調整するメディエーターが急性期に作用して炎症を解消し、炎症性に破壊された組織を結果的に再生へ向かわせる。この作用を活用した炎症性疾患治療の、歯内療法への応用を紹介した。歯髄や根尖歯周組織の炎症を調節し、組織の修復・再生に向かわせる生体反応調節性の歯内療法薬剤の研究開発が期待されている。

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    その他リンク: https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2021&ichushi_jid=J03868&link_issn=&doc_id=20210209200001&doc_link_id=10.20817%2Fjeajournal.42.1_1&url=https%3A%2F%2Fdoi.org%2F10.20817%2Fjeajournal.42.1_1&type=J-STAGE&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00007_3.gif

  • 【ペリオと○○-最新歯周治療トピックス集-】(Topic 5)ペリオとPISA 歯周組織の炎症面積で歯周病の重症度を表現しよう!

    高柴 正悟

    デンタルハイジーン   41 ( 1 )   40 - 42   2021年1月

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    記述言語:日本語   出版者・発行元:医歯薬出版(株)  

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  • 子宮内膜症の治療と妊娠を契機に進行したと疑われる慢性歯周炎患者の病態考察と治療経過

    坂井田 京佑, 大森 一弘, 小林 寛也, 山本 直史, 高柴 正悟

    岡山歯学会雑誌   39 ( 2 )   36 - 37   2020年12月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

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  • 臨床推論の教育をどうするか 病態の理解は検査と臨床推論から

    高柴 正悟

    日本口腔内科学会雑誌   26 ( 2 )   100 - 100   2020年12月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔内科学会  

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  • 侵襲性歯周炎の血液診断バイオマーカーとしての細胞外小胞由来マイクロRNAの探索

    河本 美奈, 山本 直史, 河村 麻理, 森 彩乃, 山城 圭介, 大森 一弘, 小野 喜章, 江口 傑徳, 十川 千春, 高柴 正悟

    岡山歯学会雑誌   39 ( 2 )   35 - 36   2020年12月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

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  • 真菌代謝産物(+)-terreinがマウス骨粗鬆症モデルにおける骨代謝に及ぼす影響

    坂井田 京佑, 大森 一弘, 中川 沙紀, 佐光 秀文, 亀井 千晶, 山本 総司, 小林 寛也, 山城 圭介, 山本 直史, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   153回   36 - 36   2020年11月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

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  • High Mobility Group Box 1(HMGB1)はマクロファージからのCCL2分泌を制御して抜歯窩の歯周組織再生を促進する

    井手口 英隆, 平井 杏奈, 山城 圭介, 京嶌 里沙, 青柳 浩明, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   62 ( 秋季特別 )   121 - 121   2020年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 急性期脳卒中患者における喀痰内の多剤耐性菌検出とその関連因子(横断研究)

    井上 裕貴, 松永 一幸, 山城 圭介, 高柴 正悟

    日本未病学会学術総会抄録集   27回   107 - 107   2020年10月

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    記述言語:日本語   出版者・発行元:(一社)日本未病学会  

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  • 歯の病的移動と歯周-歯内病変を併発した侵襲性歯周炎患者に対する歯周組織再生療法

    本行 令奈, 井手口 英隆, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   62 ( 秋季特別 )   142 - 142   2020年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 24年間の治療効果に関係なく高い抗P.gingivalis IgG抗体価を示す限局性侵襲性歯周炎患者

    峯柴 淳二, 峯柴 史, 岩本 義博, 高柴 正悟

    日本歯周病学会会誌   62 ( 秋季特別 )   133 - 133   2020年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 次の時代を見据えた戦略的未病対策とは 歯科疾患の未病対策は全身の未病対策に繋がる

    高柴 正悟

    日本未病学会学術総会抄録集   27回   71 - 71   2020年10月

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    記述言語:日本語   出版者・発行元:(一社)日本未病学会  

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  • ハンチントン舞踏病の広汎型慢性歯周炎患者への歯周病治療で感じた歯科衛生士の役割

    佐藤 由実, 伊藤 実有, 北村 彰子, よし田 亜紀, 苅田 奈生子, 磯島 大地, 伊東 昌洋, 長島 義之, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   62 ( 秋季特別 )   152 - 152   2020年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 歯周組織の炎症と不妊の関連性を示唆する、ある侵襲性歯周炎患者の病態生理

    大森 一弘, 河野 隆幸, 小林 寛也, 新井 英雄, 山本 直史, 高柴 正悟

    日本歯科保存学雑誌   63 ( 5 )   451 - 460   2020年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

    緒言:歯周病原細菌の感染と歯周組織の炎症が,妊娠に影響を与える可能性が報告されている.今回,不妊治療の経過が思わしくない侵襲性歯周炎患者に感染源除去の観点から専門的歯周治療を行い,自然妊娠から正常出産にいたった症例の経過をふまえ病態を考察する.症例:33歳,女性,既婚(不妊治療中).2016年9月,26の動揺および同部の自発痛を自覚し,かかりつけ歯科医院を受診した.同院でエックス線検査を受けて,重度の歯槽骨吸収があると説明された.早期の専門的歯周治療を勧められ,当科を紹介された.既往歴の特記事項はなく,不妊検査においても患者本人および夫ともに異常所見はなかった.歯周組織検査において,probing pocket depthが4mm以上の部位の割合は49.5%,bleeding on probingは47.9%,plaque control recordは3.1%,歯周炎症表面積(PISA)は2,392mm2であった.エックス線検査所見では,主訴部の26部を中心に根尖に及ぶ骨吸収像が多数存在した.歯周病原細菌に対する血清抗体価検査および歯周ポケット内細菌DNA検査ともに,Porphyromonas gingivalisの感染が強く疑われた.診断は広汎型侵襲性歯周炎(ステージIV,グレードC),二次性咬合性外傷とした.治療方針として,患者の妊娠希望に配慮して,できるかぎり早期(1年以内)の歯周環境の改善を目指すこととした.また,歯周外科治療が終了するまでの不妊治療を含めた妊娠活動を控える必要性について説明し,同意を得た.治療計画は,(1)歯周基本治療(患者教育,抜歯,局所抗菌療法を併用したスケーリング・ルートプレーニング,暫間固定),(2)歯周組織再生療法,(3)口腔機能回復治療,(4)歯周病安定期治療(SPT)とした.治療経過として,歯周治療に対する宿主反応性は非常に良く,炎症改善と歯槽骨の再生を確認した(歯周外科治療後PISA:43mm2).口腔機能回復治療中に自然妊娠し,35歳時に男児を正常出産(経腟分娩,3,240g,出産週数:38週+5日)した.考察および結論:重度のP. gingivalis感染および歯周炎症を伴う侵襲性歯周炎の罹患が,妊娠成立に影響を及ぼす可能性が示唆された.本症例のように不妊治療の経過が思わしくない場合には,歯周組織を含めた口腔状態を一度精査することが望まれる.(著者抄録)

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    その他リンク: https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2020&ichushi_jid=J01092&link_issn=&doc_id=20201110310013&doc_link_id=%2Fdo1conde%2F2020%2F006305%2F013%2F0451-0460%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fdo1conde%2F2020%2F006305%2F013%2F0451-0460%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

  • 急性期脳卒中患者における喀痰内の多剤耐性菌検出とその関連因子(横断研究)

    井上 裕貴, 松永 一幸, 坪井 綾香, 山城 圭介, 高柴 正悟

    日本歯周病学会会誌   62 ( 秋季特別 )   125 - 125   2020年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 新しい日本での歯周病治療と歯周病専門医の展開

    高柴 正悟

    日本歯周病学会会誌   62 ( 3 )   129 - 135   2020年9月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    超高齢社会の日本において医療界、特に歯科医療界がどこに向かうのか考察した。大学を含む病院が主な勤務先である医師とは異なり、歯科医師はほとんどが歯科診療所に勤務している。また、厚労省の統計によると歯科医師は医師よりも平均年齢が7歳若く、これは高齢者の割合が増加している患者への対応方法に若干の違いをもたらしている可能性がある。一方で医師と歯科医師の分布の地域差が顕著であり、来たるべき歯科医師の高齢化についても配慮が必要となる。こうした社会の変化の中で、日本歯周病学会の歯周病専門医は厚生労働省の「医療に関する広告が可能となった医師等の専門性に関する資格名」として周知されている。今後は、(一社)日本歯科専門医機構の承認も得ることには違いない。また、高度で広範囲な歯周病専門医による治療を日本国内の各地に均平化することも必要である。

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    その他リンク: https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2020&ichushi_jid=J01095&link_issn=&doc_id=20201009370001&doc_link_id=130007919193&url=https%3A%2F%2Fci.nii.ac.jp%2Fnaid%2F130007919193&type=CiNii&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00003_1.gif

  • インテグリンα3の選択的阻害による微小環境の構築と歯槽骨再生

    森 彩乃, 山本 直史, 河村 麻理, 井手口 英隆, 青柳 浩明, 中村 心, 岡本 憲太郎, 平井 杏奈, 山城 圭介, 大森 一弘, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   152回   142 - 142   2020年6月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

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  • High Mobility Group Box 1が抜歯窩治癒過程の間葉系幹細胞の遊走に及ぼす影響

    京嶌 里紗, 井手口 英隆, 山城 圭介, 平井 杏奈, 青柳 浩明, 山本 直史, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   152回   141 - 141   2020年6月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

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  • 血糖管理不良の2型糖尿病に罹患する重度慢性歯周炎患者への歯周治療

    山城 圭介, 新井 英雄, アリアス・スレマ, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   152回   55 - 55   2020年6月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

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  • 歯髄壊死した根未完成下顎第二小臼歯に対してrevascularizationを行った症例

    佐光 秀文, 高柴 正悟

    日本歯内療法学会学術大会プログラム・抄録集   41回   91 - 91   2020年6月

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    記述言語:日本語   出版者・発行元:日本歯内療法学会  

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  • 歯周病重症度マーカーとしての洗口液・唾液・歯肉溝滲出液中の短鎖脂肪酸

    畑中 加珠, 川瀬 貴博, 白波瀬 泰史, 河野 麻理, 吉田 敏之, 塚原 隆充, 落合 邦康, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   62 ( 春季特別 )   145 - 145   2020年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 真菌二次代謝産物(+)-terreinはTNF-αの発現を抑制し歯周炎マウスモデルにおける歯槽骨吸収を抑制する

    佐光 秀文, 大森 一弘, 中川 沙紀, 亀井 千晶, 坂井田 京佑, 山本 総司, 井手口 英隆, 小林 寛也, 山城 圭介, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   62 ( 春季特別 )   141 - 141   2020年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 歯周組織再生療法の違いが歯肉縁下細菌叢に及ぼす影響 侵襲性歯周炎患者の一症例

    大森 一弘, 河野 隆幸, 新井 英雄, 小林 寛也, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   62 ( 春季特別 )   165 - 165   2020年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 急性期脳卒中患者における入院時評価項目と喀痰内の薬剤耐性菌検出状況に関する調査

    井上 裕貴, 松永 一幸, 山城 圭介, 高柴 正悟

    日本口腔診断学会雑誌   33 ( 1 )   119 - 119   2020年2月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔診断学会  

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  • 歯科用接着性レジン系セメントを用いた補綴物装着によるアレルギー発症症例と検査の考察

    山城 圭介, 北川 雅恵, 岡 広子, 高柴 正悟

    日本口腔診断学会雑誌   33 ( 1 )   88 - 88   2020年2月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔診断学会  

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  • 細菌性コラゲナーゼのコラーゲン・アンカーの構造活性相関と歯周病治療への応用

    松下 治, 美間 健彦, 後藤 和義, 山本 由弥子, Caviness Perry, Sakon Joshua, 内田 健太郎, 中村 心, 岡本 健太郎, 高柴 正悟

    日本細菌学雑誌   75 ( 1 )   138 - 138   2020年1月

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    記述言語:日本語   出版者・発行元:日本細菌学会  

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  • 3根管の下顎小臼歯の歯内療法-症例報告-

    ROSALIA Arias Martinez Zulema, YAMASHIRO Keisuke, SHINODA-ITO Yuki, YAMAMOTO Tadashi, TAKASHIBA Shogo

    日本歯科保存学会学術大会プログラムおよび講演抄録集(Web)   152nd   2020年

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  • 真菌二次代謝産物terreinはマウス歯周病モデルにおける歯槽骨吸収を抑制する

    佐光 秀文, 大森 一弘, 中川 沙紀, 坂井田 京佑, 山本 総司, 青柳 浩明, 小林 寛也, 大野 充昭, 平井 公人, 山城 圭介, 山本 直史, 高柴 正悟

    有病者歯科医療   28 ( 6 )   430 - 431   2019年12月

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    記述言語:日本語   出版者・発行元:(一社)日本有病者歯科医療学会  

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  • 急性期脳卒中患者における入院時評価項目と喀痰内の薬剤耐性菌検出状況に関する調査

    井上 裕貴, 松永 一幸, 山城 圭介, 高柴 正悟

    日本口腔内科学会雑誌   25 ( 2 )   109 - 109   2019年12月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔内科学会  

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  • 歯科用接着性レジン系セメントを用いた補綴物装着によるアレルギー発症症例と検査の考察

    山城 圭介, 北川 雅恵, 岡 広子, 高柴 正悟

    日本口腔内科学会雑誌   25 ( 2 )   78 - 78   2019年12月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔内科学会  

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  • 多施設後ろ向き観察研究による臨床指標としての歯周炎症表面積の基準値

    井上 裕貴, 畑中 加珠, 山本 直史, 平田 貴久, 三辺 正人, 山本 龍生, 内藤 徹, 山本 松男, 佐藤 秀一, 石幡 浩志, 稲垣 幸司, 三谷 章雄, 中島 啓介, 漆原 譲治, 高柴 正悟

    日本歯周病学会会誌   61 ( 4 )   159 - 167   2019年12月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    歯周炎症表面積(periodontal inflamed surface area:PISA)は,歯周組織の炎症部の面積を表す新たな歯周病の臨床指標である。従来伝わりづらかった歯周病の炎症程度を歯科以外の医療従事者が理解する上で,有用な指標であると考えられる。しかし,歯周病の程度や治療によるPISAの基準は未だ不明である。そこで,本研究は,日本歯周病学会が設ける歯周病専門医・認定医の電子申請書類のデータから各治療フェーズにおけるPISAの値を調べ,歯周病治療に伴う炎症度の基準値を提案することを目的とした。8施設で取得した113症例を用いて,Nesseらの方法によって歯周ポケット深さとプロービング時の出血からPISAを算出した。その結果,PISAの中央値は,初診時1,271.4mm2,歯周基本治療終了時211.8mm2,SPT移行時52.1mm2,そして最新SPT時30.0mm2であった。また,PISAはBOPと高い相関を示し(p<0.001),BOPよりも鋭敏に治療効果を反映した。以上から,中等度以上の歯周炎においてPISAを用いると,初診時は表面積約1,500mm2の歯周組織の炎症がSPT時は100mm2未満(初診時の約7%)に減少することが明らかになった。今後,更なるデータの蓄積および詳細な分析を行いながらPISAの使用を普及させると,PISAは医科歯科連携の際に歯周病炎症を伝える指標になり得ると考える。(著者抄録)

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    その他リンク: https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2019&ichushi_jid=J01095&link_issn=&doc_id=20200128510001&doc_link_id=10.2329%2Fperio.61.159&url=https%3A%2F%2Fdoi.org%2F10.2329%2Fperio.61.159&type=J-STAGE&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00007_3.gif

  • 歯周組織の炎症と不妊との関連性を示唆する侵襲性歯周炎症例の病態生理 岡山大学病院・侵襲性歯周炎センターでの取り組み

    亀井 千晶, 大森 一弘, 小林 寛也, 山本 直史, 高柴 正悟

    岡山歯学会雑誌   38 ( 2 )   91 - 92   2019年12月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

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  • フッ素イオンによるCCNファミリー遺伝子の制御

    水川 朋美, 西田 崇, 明石 翔, 堀 彩花, 高柴 正悟, 上岡 寛, 滝川 正春, 久保田 聡

    岡山歯学会雑誌   38 ( 2 )   85 - 85   2019年12月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

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  • 基幹病院⇔診療所間の医療情報連携の現実と理想

    高柴 正悟, 岡山大学病院歯周科:医療情報部専門委員会

    医療情報学連合大会論文集   39回   86 - 87   2019年11月

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    記述言語:日本語   出版者・発行元:(一社)日本医療情報学会  

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  • 広汎型中等度慢性歯周炎患者に対して衛生管理しやすい口腔内環境を確立した症例

    田村 仁美, 清水 明美, 伊東 孝, 伊東 有希, 河野 隆幸, 高柴 正悟

    日本歯周病学会会誌   61 ( 秋季特別 )   168 - 168   2019年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 急性期脳卒中患者における喀痰内の薬剤耐性菌検出状況と関与する背景因子

    井上 裕貴, 松永 一幸, 山城 圭介, 高柴 正悟

    日本未病システム学会学術総会抄録集   26回   131 - 131   2019年10月

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    記述言語:日本語   出版者・発行元:(一社)日本未病学会  

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  • 真菌二次代謝産物terreinはマウス骨粗鬆症モデルにおいて大腿骨吸収を抑制する

    坂井田 京佑, 大森 一弘, 中川 沙紀, 佐光 秀文, 山本 総司, 小林 寛也, 平井 公人, 山城 圭介, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   61 ( 秋季特別 )   146 - 146   2019年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • High Mobility Group Box 1(HMGB1)は間葉系幹細胞の遊走を介して抜歯窩の創傷治癒を促進する

    平井 杏奈, 井手口 英隆, 山城 圭介, 青柳 浩明, 鈴木 里紗, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   61 ( 秋季特別 )   137 - 137   2019年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 二次性咬合性外傷を伴う重度慢性歯周炎患者への歯周組織再生治療の成功要因

    山本 直史, 新井 英雄, 高柴 正悟

    日本歯周病学会会誌   61 ( 秋季特別 )   165 - 165   2019年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 広汎型中等度慢性歯周炎患者の24年経過からみたSPTの重要性

    福家 教子, 佐藤 佐智子, 新井 英雄, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   61 ( 秋季特別 )   156 - 156   2019年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • Porphyromonas gingivalisのPGN_0297の機能解析

    小野 晋太郎, 中山 真彰, 大原 直也, 高柴 正悟

    日本歯周病学会会誌   61 ( 秋季特別 )   124 - 124   2019年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 【糖尿病専門医として知っておきたい歯周炎のこと】歯周病の新しい捉え方

    高柴 正悟

    月刊糖尿病   11 ( 4 )   46 - 55   2019年10月

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    記述言語:日本語   出版者・発行元:(株)医学出版  

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  • 周期的伸展刺激と静水圧刺激に対するヒト歯根膜細胞の形態と配向の変化

    藤田 彩乃, 高柴 正悟

    岡山歯学会雑誌   38 ( 1 )   1 - 12   2019年6月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

    ヒト歯根膜細胞の伸展刺激による配向性変化と、静水圧刺激による細胞形態変化について、in vitro機械刺激負荷制御システムを用いて検討した。結果、伸展刺激開始後6時間で伸展方向に対して60〜75°に配向し、静水圧刺激20MPa以上で細胞形態が変化し、40MPa以上で核形態も変化した。

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  • 歯周病原細菌を原因とするヒトと伴侶動物の犬における人獣共通感染症検査に関する研究

    田井 真砂子, 高柴 正悟

    岡山歯学会雑誌   38 ( 1 )   1 - 19   2019年6月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

    ヒトと犬における歯周病原細菌の交差感染を調べるための検査方法として、ヒトで確立されているリアルタイムPCR法による細菌DNA検査およびELISA法による血清IgG抗体検査を用い、代表的な歯周病原細菌であるP.gingivalisとP.gulaeを対象とした検査を確立することを目的に研究を行った。リアルタイムPCRで推奨される増幅DNA長150bp以下のプライマーを作製するとともに、ELISAに用いる両細菌の超音波破砕抽出抗原を作製し、犬に対する有用性について検討した。結果、作製したプライマー・抗原は犬の歯周病検査にも有用と考えられた。

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  • 歯科関連行動とIgG抗体価で示す歯周病原細菌の感染度との関連の横断研究

    坪井 綾香, 高柴 正悟

    岡山歯学会雑誌   38 ( 1 )   1 - 10   2019年6月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

    2008〜2015年に全国の日本歯周病学会会員(歯科医院)で市販の自己採血キットを用いて採取された血液から得られたIgG抗体価と、採血時の問診票に記載された歯科関連行動との関連性について検討した。対象は、全9286データ中、40歳未満と重複分を除外した5602データとした。検討の結果、年1回以上の歯科健診および歯石除去は歯周病原菌感染度と関連していることが明らかになり、この関連は特に女性と65歳未満者において顕著であった。

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  • コラーゲン結合型塩基性線維芽細胞増殖因子とコラーゲン基剤を用いた複合剤は水平性骨欠損における歯周組織再生を促進する

    中村 心, 高柴 正悟

    岡山歯学会雑誌   38 ( 1 )   1 - 14   2019年6月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

    コラーゲン結合型bFGF(CB-bFGF)は整形外科領域において骨形成促進作用が報告されており、マウスの大腿骨骨折モデルに対してCB-bFGFにコラーゲン基剤を併用すると、bFGF単独に比べて骨折部の骨形成が有意に促進されたと報告されている。そこで、CB-bFGFとコラーゲン基剤を用いた複合剤を歯周組織再生療法に応用することを考えた。しかし、応用するには口腔の解剖学的特徴すなわち、創部が開放創となりやすく、材料が流出しやすいという点を考慮する必要がある。また、整形外科領域の報告にある長管骨は中胚葉由来であるのに対し、歯周組織は外胚歯由来であり、発生学的に異なる背景をもつ。これらのことを踏まえて複合剤の効果をin vitroとin vivoで検討した結果、bFGF単独に比べて有意に歯周組織再生を促進することが確認された。

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  • Rhizopus stolonifer NBRC 30816を用いて作製した大豆発酵食品テンペに含まれる抗菌性物質の単離と同定

    伊東 昌洋, 高柴 正悟

    岡山歯学会雑誌   38 ( 1 )   1 - 16   2019年6月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

    テンペは、インドネシア原産のRhizopus属によって調製された伝統的な非塩漬け大豆発酵食品であり、発酵には、R.stolonifer、R.oligosporus、R.oryzaeがそれぞれ単独あるいは混合して使用される。テンペはグラム陽性菌に対して抗菌活性を示すと報告されていることから、著者等はテンペが口腔内細菌に対しても抗菌性をもつという仮説を立て、検証を行った。方法は、R.stolonifer、R.oligosporus、R.oryzaeそれぞれを用いて調製した3種のテンペについて、口腔内グラム陽性球菌に対する抗菌性を調べるとともに、各テンペに含まれる抗菌性物質の単離・同定を試みた。結果、3種のうちR.stoloniferで調製したテンペは抗菌性が最も強く、同テンペの粗抽出液は1mg/mL以上の濃度でS.mutansに対して抗菌性を示した。同テンペ中に含まれる抗菌性物質はリノール酸であると考えられた。

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  • 歯周組織の炎症と不妊の関連性を示唆するある侵襲性歯周炎患者の病態生理

    大森 一弘, 河野 隆幸, 小林 寛也, 新井 英雄, 山本 直史, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   150回   58 - 58   2019年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

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  • 子宮全摘出・卵巣片側摘出直後から急性化した重度慢性歯周炎症例の治療と病態考察

    坂井田 京佑, 大森 一弘, 佐光 秀文, 小林 寛也, 高知 信介, 河野 隆幸, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   61 ( 春季特別 )   157 - 157   2019年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 徹底した感染管理が垂直性骨欠損を改善する要因であった重度慢性歯周炎症例

    大久保 圭祐, 高知 信介, 本郷 昌一, 河野 隆幸, 大森 一弘, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   61 ( 春季特別 )   152 - 152   2019年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 周期性好中球減少症を有する母娘に認められた重度歯周炎の症例

    二宮 雅美, 坂本 英次郎, 成石 浩司, 生田 貴久, 高木 亮輔, 畑中 加珠, 岡本 憲太郎, 小野 晋太郎, 高柴 正悟, 湯本 浩通

    日本歯周病学会会誌   61 ( 春季特別 )   164 - 164   2019年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • コラーゲン結合型塩基性線維芽細胞成長因子はコラーゲン基剤からの徐放によって歯周組織再生を促進する

    岡本 憲太郎, 中村 心, 伊東 孝, Siddiqui Yasir Dilshad, 美間 健彦, 内田 健太郎, 大森 一弘, 山本 直史, 松下 治, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   150回   113 - 113   2019年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

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  • 細菌性コラゲナーゼのコラーゲン・アンカーと歯周組織再生への応用(Collagen anchors of bacterial collagenases and their application to periodontal tissue regeneration)

    松下 治, 美間 健彦, 後藤 和義, 山本 由弥子, Perry Caviness, Sakon Joshua, 小出 隆規, 内田 健太郎, 中村 心, 高柴 正悟

    日本細菌学雑誌   74 ( 1 )   84 - 84   2019年3月

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    記述言語:英語   出版者・発行元:日本細菌学会  

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  • 歯科から医療界へ発信する「口腔の感染・炎症・機能」に基づく歯周病の包括的臨床検査の確立

    高柴 正悟, 栗原 英見, 山崎 和久, 西村 英紀, 三辺 正人, 和泉 雄一

    日本歯科医学会誌   38   47 - 51   2019年3月

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    記述言語:日本語   出版者・発行元:日本歯科医学会  

    医科歯科連携に深く関連する歯周病関連部分を、医療全般から理解される検査として再構築することによって、プロジェクト研究テーマB「歯科診療における臨床検査の新規開発」の一部へ対応させる。これによって、21世紀の医療において、歯科医療のみならず医療全般に変革をもたらす検査法と診断体系の開発に繋がる。ペリオドンタルメディシンの考え方が普及して、口腔と全身の疾患との関連(平成28年3月に日本歯周病学会が『歯周病と全身の健康』を刊行)の中から特に糖尿病治療体系に歯周病治療が取り入れられ、さらには抗菌剤のポケット内への投与が保険収載されるようにもなった。こうした展開を歯周病が関連する他の疾患へも広げることが、歯科医療のみならず医療全般の充実をもたらす。そこで、歯周病の発症と進行、口腔機能の喪失、全身への影響という観点から、(1)感染、(2)炎症、(3)咬合力・咀嚼率、といった3つの因子を包括的に把握する。これらの3分野の組み合せで歯周病の病状を捉えるように解析方法を開発するとともに、歯周病が関連する疾患への影響度の指標となるように解析方法を検討した。(著者抄録)

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  • 真菌二次代謝産物terreinはマウス歯周病モデルにおける歯槽骨吸収を抑制する

    佐光 秀文, 大森 一弘, 中川 沙紀, 坂井田 京佑, 山本 総司, 青柳 浩明, 小林 寛也, 大野 充昭, 平井 公人, 山城 圭介, 山本 直史, 高柴 正悟

    歯科薬物療法   38 ( 2 )   128 - 128   2019年3月

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    記述言語:日本語   出版者・発行元:(一社)日本歯科薬物療法学会  

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  • 専門外来最前線 侵襲性歯周炎に対する専門外来での対応 岡山大学病院・侵襲性歯周炎センターの取り組み

    大森 一弘, 高柴 正悟

    日本歯科評論   79 ( 3 )   141 - 147   2019年3月

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    記述言語:日本語   出版者・発行元:(株)ヒョーロン・パブリッシャーズ  

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  • 【歯周病と糖尿病・代謝疾患】歯周病と菌血症・感染症

    高柴 正悟

    内分泌・糖尿病・代謝内科   48 ( 2 )   108 - 113   2019年2月

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    記述言語:日本語   出版者・発行元:(有)科学評論社  

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  • 歯周病治療介入は誤嚥性肺炎を抑制できるのか? 歯周炎と誤嚥性肺炎の関係

    高柴 正悟

    日本臨床歯周病学会会誌   36 ( 2 )   40 - 42   2019年2月

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    記述言語:日本語   出版者・発行元:(NPO)日本臨床歯周病学会  

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  • 当院における成人先天性心疾患患者の口腔状態の現況(Current Oral Condition of Patients with Adult Congenital Heart Disease in ACHD Center/Okayama University Hospital)

    大森 一弘, 杜 徳尚, 高知 信介, 山本 直史, 赤木 禎治, 伊藤 浩, 高柴 正悟

    日本成人先天性心疾患学会雑誌   8 ( 1 )   142 - 142   2019年1月

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    記述言語:英語   出版者・発行元:日本成人先天性心疾患学会  

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  • 歯科診療室における情報提示の不備が引き起こす患者への影響

    安原啓太, 杉原太郎, 柳文修, 高柴正悟

    情報科学技術フォーラム講演論文集   18th   2019年

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  • 上顎中切歯の頬側副根管の歯内療法:1症例報告

    ROSALIA Arias Martinez Zulema, SIDDIQUI Yasir Dilshad, YAMASHIRO Keisuke, SHINODA-ITO Yuki, YAMAMOTO Tadashi, TAKASHIBA Shogo

    日本歯科保存学会学術大会プログラムおよび講演抄録集(Web)   151st   2019年

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  • Porphyromonas gingivalisのPGN_0297の機能解析

    小野晋太郎, 中山真彰, 大原直也, 高柴正悟

    日本歯周病学会会誌(Web)   61   2019年

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  • 広汎型中等度慢性歯周炎患者に対して衛生管理しやすい口腔内環境を確立した症例

    田村仁美, 田村仁美, 清水明美, 清水明美, 伊東孝, 伊東有希, 河野隆幸, 高柴正悟

    日本歯周病学会会誌(Web)   61   2019年

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  • Porphyromonas gingivalisのジンジパインの機能発現におけるPGN_0297の役割

    小野 晋太郎, 高柴 正悟, 大原 直也

    岡山歯学会雑誌   37 ( 2 )   81 - 82   2018年12月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

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  • Porphyromonas gingivalis感染合併非アルコール性脂肪肝疾患に対する病態把握と治療の目安となる歯周組織検査所見の探索 多施設共同前向き観察研究

    鎌田 要平, 結束 貴臣, 清水 智子, 佐藤 五月, 青山 典生, 高柴 正悟, 中島 淳, 三辺 正人

    神奈川歯学   53 ( 抄録集 )   53 - 53   2018年12月

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    記述言語:日本語   出版者・発行元:神奈川歯科大学学会  

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  • 岡山大学病院・糖尿病教育入院患者を対象とした医科歯科連携システムの概況

    清水 由梨香, 大森 一弘, 利根 淳仁, 高知 信介, 山本 直史, 和田 淳, 高柴 正悟

    岡山歯学会雑誌   37 ( 2 )   87 - 87   2018年12月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

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  • 歯科用ユニット給水管路汚染対策に向けた自動注水型試験機の有効性評価

    岡本 憲太郎, 大久保 圭祐, 伊東 孝, 伊東 昌洋, 田井 真沙子, 中村 心, 山口 唯菜, 塩田 康祥, 河田 有祐, 大森 一弘, 山本 直史, 高柴 正悟

    岡山歯学会雑誌   37 ( 2 )   83 - 84   2018年12月

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    記述言語:日本語   出版者・発行元:岡山歯学会  

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  • 医療連携に必要な医療情報と医療連携レベル・患者個人レベルでのICT格差

    高柴 正悟, 医療情報部専門委員会

    医療情報学連合大会論文集   38回   158 - 159   2018年11月

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    記述言語:日本語   出版者・発行元:(一社)日本医療情報学会  

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  • 歯周炎罹患犬における歯周ポケット表面積推算法の確立と臨床的意義

    田村 和也, 田井 真砂子, 永原 未悠, 永原 美治, 高柴 正悟

    動物臨床医学会年次大会プロシーディング   39回 ( 2 )   131 - 132   2018年11月

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    記述言語:日本語   出版者・発行元:動物臨床医学会  

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  • コラーゲン結合型塩基性線維芽細胞成長因子とコラーゲン基剤を用いた複合剤の歯周組織再生への応用

    中村 心, 伊東 孝, 松下 治, 岡本 憲太郎, 美間 健彦, 内田 健太郎, Siddiqui Yasir Dilshad, 伊東 昌洋, 田井 真砂子, 大久保 圭祐, 山城 圭介, 大森 一弘, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   60 ( 秋季特別 )   115 - 115   2018年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 歯周病の炎症度を示す臨床的検査基準値の検討

    井上 裕貴, 畑中 加珠, 大森 一弘, 山本 直史, 三辺 正人, 高柴 正悟, 平田 貴久, 山本 龍生, 内藤 徹, 山本 松男, 佐藤 秀一, 石幡 浩志, 稲垣 幸司, 三谷 章雄, 中島 啓介, 漆原 譲治

    日本未病システム学会学術総会抄録集   25回   108 - 108   2018年10月

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    記述言語:日本語   出版者・発行元:(一社)日本未病学会  

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  • Porphyromonas gingivalis感染合併非アルコール性脂肪肝疾患に対する病態把握と治療の目安となる歯周組織検査所見の探索 多施設共同前向き観察研究

    鎌田 要平, 結束 貴臣, 清水 智子, 佐藤 五月, 青山 典生, 小林 貴, 米田 正人, 畑中 加珠, 高柴 正悟, 岩崎 知之, 栗橋 健夫, 児玉 利朗, 田村 利之, 井野 智, 中島 淳, 三辺 正人

    日本歯周病学会会誌   60 ( 秋季特別 )   115 - 115   2018年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 真菌二次代謝産物(+)-terreinはマウス実験的歯周炎モデルにおける歯槽骨吸収を抑制する

    佐光 秀文, 大森 一弘, 中川 沙紀, 坂井田 京佑, 山本 総司, 青柳 浩明, 小林 寛也, 山城 圭介, 山本 直史, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   149回   150 - 150   2018年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

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  • 歯周病原細菌によるヒトと伴侶動物イヌとの人獣共通感染症検査の研究

    田井 真砂子, 伊東 孝, 平山 晴子, 矢田 範夫, 小川 寛人, 田村 和也, 伊東 有希, 大久保 圭祐, 伊東 昌洋, 中村 心, 岡本 憲太郎, 平井 公人, 山城 圭介, 大森 一弘, 山本 直史, 樅木 勝巳, 高柴 正悟

    日本歯周病学会会誌   60 ( 秋季特別 )   135 - 135   2018年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 侵襲性歯周炎患者の血漿エクソソーム由来microRNAの発現解析

    高木 美奈, 山本 直史, 河村 麻理, 高知 信介, 山城 圭介, 大森 一弘, 江口 傑徳, 十川 千春, 高柴 正悟

    日本歯周病学会会誌   60 ( 秋季特別 )   134 - 134   2018年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 侵襲性歯周炎患者の専門外来部門連携による包括的な治療と病態解析

    高知 信介, 久保 克行, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   60 ( 秋季特別 )   148 - 148   2018年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 歯周病患者における機能指標としての咀嚼機能検査の有用性について

    宮沢 春菜, 中島 貴子, 松川 由実, 清水 伸太郎, 古市 保志, 根本 英二, 高井 英樹, 中山 洋平, 小方 頼昌, 岩崎 拓也, 石原 裕一, 大井 麻子, 齋藤 淳, 藤原 千春, 村上 伸也, 畑中 加珠, 高柴 正悟, 武田 克浩, 藤田 剛, 栗原 英見, 山崎 和久

    日本歯周病学会会誌   60 ( 秋季特別 )   136 - 136   2018年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 歯根膜細胞における機械刺激による恒常性への影響

    藤田 彩乃, 森松 賢順, 西山 雅祥, 成瀬 恵治, 高柴 正悟

    日本歯周病学会会誌   60 ( 秋季特別 )   117 - 117   2018年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 歯周病の炎症度を示す臨床的検査基準値の検討

    井上 裕貴, 畑中 加珠, 大森 一弘, 山本 直史, 三辺 正人, 高柴 正悟, 平田 貴久, 山本 龍生, 内藤 徹, 山本 松男, 佐藤 秀一, 石幡 浩志, 稲垣 幸司, 三谷 章雄, 中島 啓介, 漆原 譲治

    日本未病システム学会学術総会抄録集   25回   108 - 108   2018年10月

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    記述言語:日本語   出版者・発行元:(一社)日本未病システム学会  

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  • 化学療法施行中に口腔乾燥を訴えた進行再発乳がん患者の口腔内所見

    杉浦 裕子, 高橋 麻里子, 畑中 加珠, 田端 雅弘, 高柴 正悟

    日本歯科衛生学会雑誌   13 ( 1 )   126 - 126   2018年8月

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    記述言語:日本語   出版者・発行元:日本歯科衛生学会  

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  • 歯周炎と誤嚥性肺炎の関係

    高柴 正悟

    特定非営利活動法人日本臨床歯周病学会年次大会プログラム・講演抄録集   36回   61 - 62   2018年7月

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    記述言語:日本語   出版者・発行元:(NPO)日本臨床歯周病学会  

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  • PET(18F-FDG)/CT検査を用いた慢性歯周炎患者における歯周組織炎症の評価

    井手口 英隆, 山城 圭介, 下江 正幸, 山本 直史, 長島 義之, 高柴 正悟

    日本歯周病学会会誌   60 ( 2 )   105 - 116   2018年6月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    歯周炎に対する検査方法は多数報告されているが、歯周組織炎症の程度を客観的に評価することが出来る方法は未だに確立されていない。我々は、医科領域で広く用いられているPET(18F-FDG)/CTが炎症組織の局在と炎症強度を可視化することができることに着目した。そして、乳がんの既往を有する慢性歯周炎患者に対する歯周治療の効果を、既存の歯周組織検査方法とPET(18F-FDG)/CTとで経時的に比較検討した。歯周治療が進むに従って歯周組織炎症は消失し、BOPの割合は56%から3%に、PISAは1143mm2から27mm2に改善した。さらに、歯周治療前にPET(18F-FDG)/CTで検出された18F-FDGの顕著な集積は歯周治療後には消失した。これらの結果から、PET(18F-FDG)/CTは炎症性口腔疾患に対する新規検査方法として有用と考えられる。すなわち本症例報告は、PET(18F-FDG)/CTが医科-歯科共通の検査項目となることによって、周術期の医科-歯科連携の強化や、全身疾患を有する多くの歯周炎患者に対してさらに効果的な歯周治療を行うことが出来る可能性を提案するものである。(著者抄録)

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    その他リンク: https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2018&ichushi_jid=J01095&link_issn=&doc_id=20180706390006&doc_link_id=%2Fcp9perlo%2F2018%2F006002%2F006%2F0105-0116%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fcp9perlo%2F2018%2F006002%2F006%2F0105-0116%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

  • High Mobility Group Box 1(HMGB1)誘導性の炎症反応はマウス抜歯窩の骨治癒を促進する

    青柳 浩明, 山城 圭介, 平田 千暁, 井手口 英隆, 山崎 睦代, 河村 麻理, 鈴木 里紗, 山本 直史, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   148回   94 - 94   2018年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

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  • 真菌二次代謝産物(+)-terreinはヒト歯肉上皮細胞におけるAggregatibacter actinomycetemcomitans刺激による細胞間接着分子の発現低下を抑制する

    中村 亜里紗, 大森 一弘, 小林 寛也, 冨川 知子, 山本 総司, 中川 沙紀, 山本 直史, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   148回   153 - 153   2018年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

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  • 性別と年齢で層別した東日本大震災後の心理社会的要因と口腔内環境との関連 福島県「県民健康調査」

    坪井 綾香, 江口 依里, 大森 一弘, 山本 直史, 伊藤 達男, 長岡 憲次郎, 大平 哲也, 荻野 景規, 高柴 正悟

    日本歯周病学会会誌   60 ( 春季特別 )   120 - 120   2018年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 2型糖尿病患者に対する歯周病治療時の課題を実感した1症例

    高橋 麻里子, 大森 一弘, 千神 八重子, 山本 直史, 高柴 正悟

    糖尿病   61 ( 5 )   359 - 359   2018年5月

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    記述言語:日本語   出版者・発行元:(一社)日本糖尿病学会  

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  • アレッ、あなたも…? 「口臭を捉える検査」

    高柴 正悟

    日本口臭学会会誌   9 ( 1 )   37 - 38   2018年5月

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    記述言語:日本語   出版者・発行元:日本口臭学会  

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  • 歯科治療介入が生活習慣の改善につながった糖尿病関連性歯周炎患者の一症例

    志茂 加代子, 河村 麻理, 大森 一弘, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   60 ( 春季特別 )   162 - 162   2018年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 精神疾患を有する重度慢性歯周炎患者に対する侵襲度が必要最小限の治療例

    村田 裕美, 高柴 正悟

    日本歯周病学会会誌   60 ( 春季特別 )   153 - 153   2018年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 歯周病専門医申請症例の臨床データを用いた歯周炎症表面積(PISA)の基準値の検討

    畑中 加珠, 平田 貴久, 三辺 正人, 山本 龍生, 内藤 徹, 山本 松男, 佐藤 秀一, 石幡 浩志, 稲垣 幸司, 三谷 章雄, 中島 啓介, 漆原 譲治, 高柴 正悟

    日本歯周病学会会誌   60 ( 春季特別 )   120 - 120   2018年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 糖尿病重症度により層別したPISAによる歯周病重症度と動脈硬化との関連

    杉 典子, 坪井 綾香, 畑中 加珠, 江口 依里, 荻野 景規, 吉良 友里, 三浦 晶子, 土居 健太郎, 高柴 正悟

    日本歯周病学会会誌   60 ( 春季特別 )   120 - 120   2018年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 上顎中切歯の歯内-歯周病変に対して部分矯正治療と自家骨移植術を行い歯科インプラント治療を回避した一症例

    鈴木 里紗, 井手口 英隆, 本郷 昌一, 大森 一弘, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   60 ( 春季特別 )   144 - 144   2018年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 糖尿病教育入院患者の歯周病重症度と糖尿病合併症との関連

    杉 典子, 坪井 綾香, 畑中 加珠, 江口 依里, 荻野 景規, 高柴 正悟, 吉良 友里, 三浦 晶子, 土居 健太郎

    糖尿病   61 ( Suppl.1 )   S - 184   2018年4月

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    記述言語:日本語   出版者・発行元:(一社)日本糖尿病学会  

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  • 歯科用ユニット給水管路汚染対策用の注水制御シミュレータの評価

    岡本 憲太郎, 大久保 圭祐, 伊東 孝, 山本 一郎, 水谷 元, 伊東 昌洋, 田井 真沙子, 中村 心, 塩田 康祥, 河田 有祐, 大森 一弘, 山本 直史, 高柴 正悟

    日本口腔機能水学会誌   19 ( 1 )   34 - 35   2018年3月

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    記述言語:日本語   出版者・発行元:日本口腔機能水学会  

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  • 感染、炎症、機能の観点から見た口腔検査指標の歯周病治療に対する有用性 パイロット研究

    畑中 加珠, 片山 広大, 井上 裕貴, 坂井田 京佑, 清水 由梨香, 鈴木 里紗, 高木 美奈, 山本 直史, 高柴 正悟

    日本口腔検査学会雑誌   10 ( 1 )   58 - 63   2018年3月

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    記述言語:日本語   出版者・発行元:(一社)日本口腔検査学会  

    目的:歯周病治療の効果を「感染」、「炎症」、そして「機能」の3つの観点から評価して、治療効果を示す指標を探った。方法:歯周病治療を終えて安定期治療に至った7症例において、歯周局所の歯周病原細菌数と9菌種の血清IgG抗体価、歯周ポケット深さとプロービング時の出血から算出する歯周炎症表面積(PISA)、そして咀嚼機能の簡易指標として残存歯の動揺度を測定した。これら4つの指標の変動を治療時期の一定ポイントで調べ、それらの相互関係を検討した。結果:歯周治療が進むにしたがって、PISA、歯の動揺度およびPorphyromonas gingivalis(Pg)に対する抗体価の数値は低下し、7症例の各時期における平均値をとると、経時的な有意差が認められた(P<0.05)。しかしながら、他の菌種に対する抗体価や歯周局所の細菌数には増加するものもみられた。結論:「感染」、「炎症」、「機能」の3つの観点から歯周病治療の効果を評価する指標として、抗Pg抗体価、PISA、そして歯の動揺度を用いることは有用であった。(著者抄録)

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    その他リンク: https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2018&ichushi_jid=J05670&link_issn=&doc_id=20180514360008&doc_link_id=http%3A%2F%2Fhdl.handle.net%2F10130%2F4539&url=http%3A%2F%2Fhdl.handle.net%2F10130%2F4539&type=%93%8C%8B%9E%8E%95%89%C8%91%E5%8Aw%81F%93%8C%8B%9E%8E%95%89%C8%91%E5%8Aw%8Aw%8Fp%8B%40%8A%D6%83%8A%83%7C%83W%83g%83%8A%81FIRUCAA%40TDC&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F80092_3.gif

  • 多職種と連携した脳血管障害後遺症患者の管理における歯科衛生士の役割

    森本 祥代, 後藤 絢香, 大森 一弘, 高柴 正悟

    日本歯科評論   78 ( 2 )   123 - 131   2018年2月

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    記述言語:日本語   出版者・発行元:(株)ヒョーロン・パブリッシャーズ  

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  • 歯学の行方 歯周病の客観的な検査方法の課題 一般医療との連携と未病への発展を目指した"夢"

    高柴 正悟

    日本歯科評論   78 ( 1 )   21 - 23   2018年1月

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    記述言語:日本語   出版者・発行元:(株)ヒョーロン・パブリッシャーズ  

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  • 2型糖尿病患者に対する歯周治療の効果と課題を実感した一症例

    高橋 麻里子, 大森 一弘, 千神 八重子, 山本 直史, 高柴 正悟

    日本歯科評論   78 ( 1 )   141 - 150   2018年1月

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    記述言語:日本語   出版者・発行元:(株)ヒョーロン・パブリッシャーズ  

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  • コラーゲン結合型塩基性線維芽細胞成長因子とコラーゲン基剤を用いた複合剤の歯周組織再生への応用

    中村心, 伊東孝, 松下治, 岡本憲太郎, 美間健彦, 内田健太郎, SIDDIQUI Yasir Dilshad, 伊東昌洋, 田井真砂子, 大久保圭祐, 山城圭介, 大森一弘, 山本直史, 高柴正悟

    日本歯周病学会会誌(Web)   60   2018年

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  • 人工関節置換術の周術期における早期からの口腔感染管理が奏功した慢性歯周炎患者の一症例

    小川 侑子, 山本 総司, 佐藤 公麿, 峠 亜也香, 宮岡 満奈, 向井 麻里子, 児玉 由佳, 竹本 奈奈, 大森 一弘, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   59 ( 秋季特別 )   232 - 232   2017年11月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 医科歯科連携を目指した歯周病の検査と診断 歯周病原細菌の血清抗体価検査の伝統と革新

    高柴 正悟

    日本歯周病学会会誌   59 ( 秋季特別 )   121 - 121   2017年11月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 口腔バイオフィルム感染症を制御するパウダー状の天然食品の探索

    伊東 昌洋, 伊東 孝, 河田 有祐, 塩田 康祥, 大久保 圭祐, 田井 真砂子, 中村 心, 岡本 憲太郎, 青木 秀之, 二井 広平, 宮島 彩, 大森 一弘, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   59 ( 秋季特別 )   190 - 190   2017年11月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 中等度慢性歯周炎患者に対して歯周組織再生療法を行った術後5年経過症例

    大江 丙午, 高柴 正悟

    日本歯周病学会会誌   59 ( 秋季特別 )   218 - 218   2017年11月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 口腔細菌の特定と口腔治療が急性椎前部膿瘍の治癒に奏功した症例

    松永 一幸, 山城 圭介, 平田 千暁, 猪原 健, 大久保 圭祐, 磯島 大地, 坂井田 京佑, 大森 一弘, 山本 直史, 高柴 正悟, 竹丸 誠, 下江 豊, 栗山 勝

    日本歯周病学会会誌   59 ( 秋季特別 )   193 - 193   2017年11月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 血清IgG抗体価検査がスクリーニング、早期診断および管理につながった侵襲性歯周炎患者の22年間経過症例

    大森 一弘, 大山 秀樹, 河野 隆幸, 冨川 知子, 清水 明美, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   59 ( 秋季特別 )   225 - 225   2017年11月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 咬合挙上と自家骨移植が治療ポイントとなった重度慢性歯周炎患者症例

    中村 心, 井手口 英隆, 佐々木 禎子, 峯柴 淳二, 下江 正幸, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   59 ( 秋季特別 )   218 - 218   2017年11月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 家族性リポ蛋白リパーゼ欠損症および2型糖尿病に罹患した重度慢性歯周炎患者に対する非外科的歯周治療の効果を実感した一症例

    千神 八重子, 大森 一弘, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   59 ( 秋季特別 )   233 - 233   2017年11月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 歯周病罹患の有無によるアテローム性動脈硬化病変の細菌叢のメタゲノム解析

    磯島 大地, 山城 圭介, 松永 一幸, 大森 一弘, 山本 直史, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   147回   125 - 125   2017年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

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  • 歯科用ユニット給水管路汚染対策用の自動注水制御シミュレータの評価

    大久保 圭祐, 伊東 孝, 山本 一郎, 水谷 元, 伊東 昌洋, 田井 真砂子, 中村 心, 岡本 憲太郎, 塩田 康祥, 河田 有祐, 大森 一弘, 山本 直史, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   147回   134 - 134   2017年10月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

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  • 初回外来化学療法患者の口腔カンジダ感染量が口腔内の疼痛やQOLに及ぼす影響

    杉浦 裕子, 山城 圭介, 畑中 加珠, 高坂 由紀奈, 小倉 早妃, 益成 美保, 大森 一弘, 曽我 賢彦, 佐々木 朗, 高柴 正悟

    日本歯科衛生学会雑誌   12 ( 1 )   84 - 84   2017年8月

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    記述言語:日本語   出版者・発行元:日本歯科衛生学会  

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  • 関節・成長板軟骨細胞におけるセロトニン(5-HT)によるCCN2産生の差別的制御メカニズム

    堀 綾花, 西田 崇, 高柴 正悟, 久保田 聡, 滝川 正春

    日本骨代謝学会学術集会プログラム抄録集   35回   167 - 167   2017年7月

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    記述言語:日本語   出版者・発行元:(一社)日本骨代謝学会  

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  • 患者視点からの歯周組織再生療法の選択基準

    畑中 加珠, 下江 正幸, 大森 一弘, 山本 直史, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   146回   170 - 170   2017年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

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  • インテグリンサブユニットの選択的制御による歯根膜細胞の遊走促進

    河村 麻理, 山本 直史, 山城 圭介, 平田 千暁, 青柳 浩明, 井手口 英隆, 大森 一弘, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   146回   154 - 154   2017年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

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  • 真菌二次代謝産物(+)-terreinはRANKL誘導性破骨細胞分化におけるNFATc1の発現を抑制する

    中川 沙紀, 大森 一弘, 山本 総司, 小林 寛也, 河村 麻理, 中村 亜里紗, 山本 直史, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   146回   54 - 54   2017年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

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  • 歯肉縁下細菌と血清抗体価による慢性歯周炎進行の予知判定 24ヵ月多施設前向きコホート研究

    両角 俊哉, 中川 種昭, 野口 和行, 原 宜興, 西村 英紀, 梅田 誠, 野口 俊英, 吉成 伸夫, 沼部 幸博, 伊藤 公一, 和泉 雄一, 小方 頼昌, 三邉 正人, 齋藤 淳, 佐藤 聡, 高橋 慶壮, 川浪 雅光, 花田 信弘, 高柴 正悟, 吉江 弘正, 角田 衣理加, 中村 利明, 吉村 篤利, 前田 勝正, 藤瀬 修, 上田 雅俊, 河野 智生, 福田 光男, 横井 隆, 高橋 美穂, 深谷 千絵, 関野 愉, 菅野 直之, 小林 宏明, 高井 英樹, 中山 洋平, 高野 聡美, 山田 了, 大井 麻子, 奥田 倫子, 横山 智子, 阿部 祐三, 鈴木 史彦, 菅谷 勉, 野村 義明

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   146回   174 - 174   2017年5月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

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  • 2型糖尿病患者に対する歯周治療の効果とSPT時の課題を実感した一症例

    高橋 麻里子, 大森 一弘, 千神 八重子, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   59 ( 春季特別 )   170 - 170   2017年4月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 多職種と連携した脳血管障害後遺症患者の管理における歯科衛生士の役割

    森本 祥代, 後藤 絢香, 赤枝 久美, 森石 真代, 佐藤 由美, 長島 義之, 大森 一弘, 高柴 正悟

    日本歯周病学会会誌   59 ( 春季特別 )   172 - 172   2017年4月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • HMGB1が歯槽骨治癒に及ぼす影響

    青柳 浩明, 山城 圭介, 井手口 英隆, 平田 千暁, 河村 麻理, 下江 正幸, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   59 ( 春季特別 )   124 - 124   2017年4月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 専門医を求めて受診した広汎型重度慢性歯周炎の一症例

    岩本 義博, 鵜川 祐樹, 佐藤 毅, 冨川 和哉, 湊 ゆかり, 高柴 正悟

    日本歯周病学会会誌   59 ( 春季特別 )   158 - 158   2017年4月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 広汎型侵襲性歯周炎患者の治療後7年経過症例

    鵜川 祐樹, 岩本 義博, 佐藤 毅, 冨川 和哉, 湊 ゆかり, 高柴 正悟

    日本歯周病学会会誌   59 ( 春季特別 )   158 - 158   2017年4月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • PET(18F-FDG)/CT検査を用いた慢性歯周炎患者における歯周組織炎症の評価

    井手口 英隆, 森本 祥代, 後藤 絢香, 長島 義之, 高柴 正悟

    日本歯周病学会会誌   59 ( 春季特別 )   163 - 163   2017年4月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 矯正治療と自家骨移植を用いた再生療法を行い良好な経過が得られた慢性歯周炎患者の一症例

    山城 圭介, 河野 隆幸, 下江 正幸, 高柴 正悟

    日本歯周病学会会誌   59 ( 春季特別 )   159 - 159   2017年4月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 真菌代謝産物terreinはAggregatibacter actinomycetemcomitans歯肉上皮感染時のIL-8産生を抑制する

    中村 亜里紗, 大森 一弘, 小林 寛也, 山本 総司, 中川 沙紀, 冨川 知子, 峯柴 史, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   59 ( 春季特別 )   137 - 137   2017年4月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 歯周炎と全身疾患(糖尿病・関節リウマチ)に共通するリスク遺伝子の解析

    小林 哲夫, 木戸 淳一, 石原 裕一, 大森 一弘, 三谷 章雄, 高柴 正悟, 永田 俊彦, 吉江 弘正

    日本歯周病学会会誌   59 ( 春季特別 )   128 - 128   2017年4月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 歯肉縁下細菌と血清抗体価を用いた慢性歯周炎進行の評価 24ヵ月多施設前向きコホート研究

    両角 俊哉, 角田 衣理加, 野村 義明, 中川 種昭, 野口 和行, 原 宜興, 西村 英紀, 梅田 誠, 野口 俊英, 吉成 伸夫, 沼部 幸博, 伊藤 公一, 和泉 雄一, 小方 頼昌, 三邉 正人, 齋藤 淳, 佐藤 聡, 高橋 慶壮, 川浪 雅光, 花田 信弘, 高柴 正悟, 吉江 弘正

    日本歯周病学会会誌   59 ( 春季特別 )   145 - 145   2017年4月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • 歯周病罹患の有無によるアテローム性動脈硬化病変部のマイクロバイオームの違い

    磯島 大地, 山城 圭介, 松永 一幸, 大森 一弘, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   59 ( 春季特別 )   142 - 142   2017年4月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

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  • メカノストレスによる歯周組織リモデリング機構の解明

    藤田 彩乃, 森松 賢順, 高橋 賢, 高柴 正悟, 成瀬 恵治

    日本生理学雑誌   79 ( 1 )   44 - 44   2017年2月

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    記述言語:日本語   出版者・発行元:(一社)日本生理学会  

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  • 歯周病対策としてのバイオフィルム制御の展望

    高柴 正悟

    日本歯周病学会会誌   58 ( 4 )   229 - 235   2017年1月

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    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    歯周病対策の基本である口腔細菌バイオフィルムの制御について概説した。バイオフィルムの形成は、細菌の初期付着、不可逆性付着、バイオフィルムの形成、増大と成熟、バイオフィルムからの細菌の分散、といった5段階であるとされる。バイオフィルムを形成すると、細菌間での情報交換であるquorum sensingが活発になり、浮遊状態である細菌の代謝活性とは異なるものになる。口腔に棲息する常在細菌叢を、質的に各細菌種の構成割合を維持し、一方で量的には各細菌種の細菌数を減少させることが,細菌と生体のバランスを考えると望ましいと考えられ、相利共生(symbiosis)の概念が普及してきた。また、細菌叢内でのquorum sensingを理解することで、細菌間での連携を攪乱する方法でバイオフィルムを阻止することも考えられている。

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    その他リンク: https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2017&ichushi_jid=J01095&link_issn=&doc_id=20170322320002&doc_link_id=%2Fcp9perlo%2F2016%2F005804%2F002%2F0229-0235%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fcp9perlo%2F2016%2F005804%2F002%2F0229-0235%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

  • 女性特有のライフイベントを意識した広汎型侵襲性歯周炎患者に対する長期的な歯周治療支援

    稲垣 明子[高橋], 大森 一弘, 村内 利光, 高柴 正悟

    日本歯科評論   77 ( 1 )   123 - 131   2017年1月

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    記述言語:日本語   出版者・発行元:(株)ヒョーロン・パブリッシャーズ  

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  • 新規除鉄剤スーパーポリフェノール(SP)を応用した口腔感染制御システムの開発

    伊東有希, 大森一弘, 大原利章, 高柴正悟

    日本鉄バイオサイエンス学会学術集会プログラム・抄録集   41st   2017年

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  • 口腔バイオフィルム感染症を制御するパウダー状の天然食品の探索

    伊東昌洋, 伊東孝, 河田有祐, 塩田康祥, 大久保圭祐, 田井真砂子, 中村心, 岡本憲太郎, 青木秀之, 二井広平, 宮島彩, 大森一弘, 山本直史, 高柴正悟

    日本歯周病学会会誌(Web)   59   2017年

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  • 歯肉縁下細菌と血清抗体価を用いた慢性歯周炎進行の評価:24ヵ月多施設前向きコホート研究

    両角俊哉, 角田衣理加, 野村義明, 中川種昭, 野口和行, 原宜興, 西村英紀, 梅田誠, 野口俊英, 吉成伸夫, 沼部幸博, 伊藤公一, 和泉雄一, 小方頼昌, 三邉正人, 齋藤淳, 佐藤聡, 高橋慶壮, 川浪雅光, 花田信弘, 高柴正悟, 吉江弘正

    日本歯周病学会会誌(Web)   59   2017年

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