2021/10/14 更新

写真a

タカシバ ショウゴ
髙柴 正悟
TAKASHIBA Shogo
所属
医歯薬学域 教授
職名
教授
プロフィール
歯や口の健康だけではなく全身の健康にいろいろと関係する「歯周病」の研究を,細菌学,免疫学,分子細胞生物学の観点によって,基礎から臨床まで幅広く行っています。 特に,感染の制御,炎症の制御,そして組織再生の制御の3分野での視点でもって,研究を発展させています。 最近では,臨床現場の先生方と連携した臨床疫学・観察・介入の研究へと発展させ,さらには種々の製品開発に関わる研究へと視野を拡大しています。
外部リンク

学位

  • 歯学博士 ( 1990年3月   岡山大学 )

  • 歯学士 ( 1986年3月   岡山大学 )

研究キーワード

  • 歯周病学

  • 歯内療法学

  • Periodontal Science (Periodontology & Endodontology)

  • 歯学

研究分野

  • ライフサイエンス / 保存治療系歯学

  • ライフサイエンス / 保存治療系歯学

学歴

  • 岡山大学   Graduate School of Dentistry   Periodontology and Endodontology

    1986年4月 - 1990年3月

      詳細を見る

  • 岡山大学   Graduate School, Division of Dental Research  

    - 1990年

      詳細を見る

  • 岡山大学    

    - 1990年

      詳細を見る

    国名: 日本国

    researchmap

  • 岡山大学   Dental School   Dentistry

    1980年4月 - 1986年2月

      詳細を見る

  • 岡山大学   歯学部   歯学科

    - 1986年

      詳細を見る

    国名: 日本国

    researchmap

  • 岡山大学   Faculty of Dentistry  

    - 1986年

      詳細を見る

▼全件表示

経歴

  • Okayama University (Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Periodontal Science)   Professor

    2005年4月 - 現在

      詳細を見る

  • - 岡山大学医歯薬学総合研究科病態制御科学専攻 教授

    2005年

      詳細を見る

  • Okayama University (Graduate School of Medicine and Dentistry, Periodontal Science)   Professor

    2002年4月 - 2005年3月

      詳細を見る

  • Okayama University (Graduate School of Medicine and Dentistry, Periodontal Science)   Associate Professor

    2001年4月 - 2002年3月

      詳細を見る

  • Okayama University (Dental School, Periodontology & Endodontology)   Associate Professor

    1995年11月 - 2001年3月

      詳細を見る

  • Okayama University (Dental School, Periodontology & Endodontology)   Assistant Professor

    1994年12月 - 1995年11月

      詳細を見る

  • Eastman Dental Center (Periodontology: Prof. Van Dyke), Rochester, NY, USA   Visiting Scientist

    1992年4月 - 1994年11月

      詳細を見る

  • Okayama University (University Hospital of Dentistry, Periodontics & Endodontics)   Assistant Professor

    1990年4月 - 1992年3月

      詳細を見る

▼全件表示

所属学協会

▼全件表示

委員歴

  • 国際歯周病アカデミー   Board Menber  

    2017年   

      詳細を見る

    団体区分:学協会

    国際歯周病アカデミー

    researchmap

  • 日本歯科保存学会   常任理事,医療合理化委員会委員長  

    2017年   

      詳細を見る

    団体区分:学協会

    日本歯科保存学会

    researchmap

  • 日本歯周病学会   常任理事,専門医委員会医員長  

    2017年   

      詳細を見る

    団体区分:学協会

    日本歯周病学会

    researchmap

  • 日本歯周病学会   常任理事,研究委員会医員長  

    2011年 - 2014年   

      詳細を見る

    団体区分:学協会

    日本歯周病学会

    researchmap

  • 日本口腔検査学会   理事  

    2011年   

      詳細を見る

    団体区分:学協会

    日本口腔検査学会

    researchmap

  • 日本歯周病学会   2009年(第52回)春季日本歯周病学会 学術大会大会長  

    2009年 - 2011年   

      詳細を見る

    団体区分:学協会

    日本歯周病学会

    researchmap

  • 日本歯周病学会   常任理事,用語委員会委員長  

    2009年 - 2010年   

      詳細を見る

    団体区分:学協会

    日本歯周病学会

    researchmap

  • 日本歯科保存学会   常任理事,編集委員会委員長  

    2007年 - 2010年   

      詳細を見る

    団体区分:学協会

    日本歯科保存学会

    researchmap

  • 日本歯周病学会   理事  

    2007年   

      詳細を見る

    団体区分:学協会

    日本歯周病学会

    researchmap

  • 日本歯周病学会   理事  

    2007年   

      詳細を見る

    団体区分:学協会

    日本歯周病学会

    researchmap

  • 日本未病システム学会   評議員  

    2006年 - 2015年   

      詳細を見る

    団体区分:学協会

    日本未病システム学会

    researchmap

  • 日本歯科保存学会   理事  

    2002年   

      詳細を見る

    団体区分:学協会

    日本歯科保存学会

    researchmap

  • 岡山歯学会   理事  

    2002年   

      詳細を見る

    団体区分:学協会

    岡山歯学会

    researchmap

  • 国際歯科研究学会   Editorial Board Member  

    2001年 - 2004年   

      詳細を見る

    団体区分:学協会

    国際歯科研究学会

    researchmap

  • 国際歯科研究学会   Abstract Reviewer  

    2000年 - 2004年   

      詳細を見る

    団体区分:学協会

    国際歯科研究学会

    researchmap

▼全件表示

 

論文

  • Functionalized Graphene Oxide Shields Tooth Dentin from Decalcification. 国際誌

    M Z I Nizami, Y Nishina, T Yamamoto, Y Shinoda-Ito, S Takashiba

    Journal of dental research   99 ( 2 )   182 - 188   2020年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    This in vitro study assessed the efficacy of functionalized graphene oxide (f-GO) nanocomposites on the decalcification of dentin, because dental caries of the root surface is becoming one of the new problems in aged society. Hydroxyapatite plates (HAP) and dentin slices were coated with f-GO nanocomposites by comparing them to silver diamine fluoride as a positive control, then treated with decalcification solutions such as ethylenediaminetetraacetic acid and citrate at 37°C for 24 h. Scanning electron microscopy (SEM) revealed significant protection of the surface morphology of HAP and dentin. On the other hand, a cariogenic Streptococcus mutans growth was inhibited by f-GO nanocomposites. In addition, cytotoxicity of them to epithelial cells was much less than that of povidone-iodine, which is commonly used for oral disinfectant. We synthesized 5 different f-GO nanocomposites such as GO-silver (Ag), GO-Ag-calcium fluoride (CaF2), GO-CaF2, GO-zinc, and GO-tricalcium phosphate (Ca3(PO4)2). They were standardized by evaluating under SEM, transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), thermogravimetry analysis (TGA), and Raman spectra after being synthesized in an aseptic technique. The abilities of GO-Ag, GO-Ag-CaF2, and GO-CaF2 nanocomposites were most preventive for decalcification. In addition, GO-Ag and GO-Ag-CaF2 almost completely inhibited S. mutans growth. However, they did not exhibit cytotoxicity to epithelial cells except at the highest concentration (0.1 w/v%) of GO-Ag and GO-Ag-CaF2. Furthermore, these f-GO nanocomposites exhibited less or no discoloration of dentin, although commonly used silver diamine fluoride causes discoloration of dentin to black. Thus, these f-GO nanocomposites are useful to protect dental caries on the tooth root that becomes a social problem in aged society.

    DOI: 10.1177/0022034519894583

    PubMed

    researchmap

  • 多施設後ろ向き観察研究による臨床指標としての歯周炎症表面積の基準値 査読

    井上 裕貴, 畑中 加珠, 山本 直史, 平田 貴久, 三辺 正人, 山本 龍生, 内藤 徹, 山本 松男, 佐藤 秀一, 石幡 浩志, 稲垣 幸司, 三谷 章雄, 中島 啓介, 漆原 譲治, 高柴 正悟

    日本歯周病学会会誌   61 ( 4 )   159 - 167   2019年12月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    歯周炎症表面積(periodontal inflamed surface area:PISA)は,歯周組織の炎症部の面積を表す新たな歯周病の臨床指標である。従来伝わりづらかった歯周病の炎症程度を歯科以外の医療従事者が理解する上で,有用な指標であると考えられる。しかし,歯周病の程度や治療によるPISAの基準は未だ不明である。そこで,本研究は,日本歯周病学会が設ける歯周病専門医・認定医の電子申請書類のデータから各治療フェーズにおけるPISAの値を調べ,歯周病治療に伴う炎症度の基準値を提案することを目的とした。8施設で取得した113症例を用いて,Nesseらの方法によって歯周ポケット深さとプロービング時の出血からPISAを算出した。その結果,PISAの中央値は,初診時1,271.4mm2,歯周基本治療終了時211.8mm2,SPT移行時52.1mm2,そして最新SPT時30.0mm2であった。また,PISAはBOPと高い相関を示し(p<0.001),BOPよりも鋭敏に治療効果を反映した。以上から,中等度以上の歯周炎においてPISAを用いると,初診時は表面積約1,500mm2の歯周組織の炎症がSPT時は100mm2未満(初診時の約7%)に減少することが明らかになった。今後,更なるデータの蓄積および詳細な分析を行いながらPISAの使用を普及させると,PISAは医科歯科連携の際に歯周病炎症を伝える指標になり得ると考える。(著者抄録)

    researchmap

  • Acceleration of bone regeneration of horizontal bone defect in rats using collagen-binding basic fibroblast growth factor combined with collagen scaffolds. 査読 国際誌

    Shin Nakamura, Takashi Ito, Kentaro Okamoto, Takehiko Mima, Kentaro Uchida, Yasir D Siddiqui, Masahiro Ito, Masako Tai, Keisuke Okubo, Keisuke Yamashiro, Kazuhiro Omori, Tadashi Yamamoto, Osamu Matsushita, Shogo Takashiba

    Journal of periodontology   90 ( 9 )   1043 - 1052   2019年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Basic fibroblast growth factor (bFGF) has been applied for periodontal regeneration. However, the application depends on bone defect morphology because bFGF diffuses rapidly from defect sites. In a previous study, collagen-binding bFGF (CB-bFGF) has been shown to enhance bone formation by collagen-anchoring in the orthopedic field. The aim of this study is to demonstrate the efficacy of CB-bFGF with collagen scaffolds in bone regeneration of horizontal bone defect. METHODS: Cell proliferation activity and collagen binding activity of CB-bFGF was confirmed by WST-8 assay and collagen binding assay, respectively. The retention of CB-bFGF in the collagen sheet (CS) was measured by fluorescence imaging. The rat horizontal alveolar bone defect model was employed to investigate the efficacy of CB-bFGF with collagen powder (CP). After 4 and 8 weeks, the regenerative efficacy was evaluated by microcomputed tomography, histological, and immunohistochemical analyses. RESULTS: CB-bFGF had a comparable proliferation activity to bFGF and a collagen binding activity. CB-bFGF was retained in CS longer than bFGF. At 8 weeks postoperation, bone volume, bone mineral content, and new bone area in CB-bFGF/CP group were significantly increased compared with those in other groups. Furthermore, epithelial downgrowth was significantly suppressed in CB-bFGF/CP group. At 4 weeks, the numbers of osteocalcin, proliferating cell nuclear antigen, and osteopontin-positive cells at the regeneration site in CB-bFGF/CP group were greater than those in other groups. CONCLUSIONS: CB-bFGF/CP effectively promoted bone regeneration of horizontal bone defect possibly by sustained release of bFGF. The potential of CB-bFGF composite material for improved periodontal regeneration in vertical axis was shown.

    DOI: 10.1002/JPER.18-0674

    PubMed

    researchmap

  • Resolvin D2 Induces Resolution of Periapical Inflammation and Promotes Healing of Periapical Lesions in Rat Periapical Periodontitis. 査読 国際誌

    Siddiqui YD, Omori K, Ito T, Yamashiro K, Nakamura S, Okamoto K, Ono M, Yamamoto T, Van Dyke TE, Takashiba S

    Frontiers in immunology   10   307 - 307   2019年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3389/fimmu.2019.00307

    PubMed

    researchmap

  • Malnutrition delayed wound healing after tooth extraction by HMGB1-related prolonged inflammation. 国際誌

    Yao Zhang, Hidetaka Ideguchi, Hiroaki Aoyagi, Keisuke Yamashiro, Tadashi Yamamoto, Masahiro Nishibori, Shogo Takashiba

    International immunopharmacology   96   107772 - 107772   2021年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Malnutrition causes prolonged inflammation, resulting in delayed wound healing. High mobility group box-1 (HMGB1) is a damage-associated molecular pattern that is present in the nuclei of macrophages and is secreted into the extracellular milieu in response to stimuli. It stimulates the production of interleukin-1β (IL-1β) through the receptors for advanced glycation end products (RAGE), inducing an inflammatory response, which is an essential response to initiate wound healing. We hypothesized that malnutrition may interfere with this cascade, causing abnormal inflammation and ultimately delaying wound healing. We used tooth-extracted mice with malnutrition fed with low-casein diet for two weeks. On days 3 and 7 after tooth extraction, the wound tissue was histologically observed and analyzed for several factors in the inflammation-regeneration lineage, including IL-1β, mesenchymal stem cells, myeloperoxidase activity, HMGB1, macrophage polarization, and adenosine 5-triphosphate (ATP). On day 7, delayed wound healing was observed with the following findings under malnutrition conditions: decreased mRNA expression of genes for regeneration and mesenchymal stem cell (MSC) accumulation, an obvious increase in myeloperoxidase and IL-1β mRNA expression, an increase in HMGB1 levels, and an increase in ATP concentration in tissues with elevated proportion of M2 macrophages. These results suggest that the significantly increased secretion of HMGB1 associated with the upregulated production of ATP and IL-1β secretion via the RAGE pathway may interfere with the resolution of inflammation and wound healing under the state of malnutrition.

    DOI: 10.1016/j.intimp.2021.107772

    PubMed

    researchmap

  • Association between Psychosocial Factors and Oral Symptoms among Residents in Fukushima after the Great East Japan Earthquake: A Cross-Sectional Study from the Fukushima Health Management Survey. 国際誌

    Narumi Funakubo, Ayaka Tsuboi, Eri Eguchi, Fumikazu Hayashi, Masaharu Maeda, Hirooki Yabe, Seiji Yasumura, Kenji Kamiya, Shogo Takashiba, Tetsuya Ohira, Mental Health Group Of The Fukushima Health Management Survey

    International journal of environmental research and public health   18 ( 11 )   2021年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Oral health is closely related to subjective general health and systemic diseases. This cross-sectional study aimed to identify the factors related to oral symptoms and their worsening in relation to psychosocial factors after the Great East Japan Earthquake. In this study, 64,186 residents aged 15-101 years old, who experienced the earthquake on 11 March 2011, were surveyed regarding their oral symptoms; psychological factors, such as post-traumatic reactions and psychological distress; and social factors such as evacuation, work change, and loss of a close person; history of systemic diseases; and lifestyle. Binomial logistic regression analysis was used to calculate odds ratios, and 95% confidence intervals were established for each factor associated with prevalent and exacerbated oral symptoms. The proportions of participants with prevalent and exacerbated oral symptoms were 10.3% and 1.6%, respectively. The multivariate odds ratios and 95% CI of psychosocial factors associated with exacerbated oral symptoms were as follows: post-traumatic stress disorder symptoms, 2.24 (1.64-3.06); work changes, 1.88 (1.34-2.65); history of dyslipidemia, 1.74 (1.27-2.39); and subjective current poor health condition, 2.73 (2.00-3.75). Psychological factors, social factors, and physical factors were associated with both prevalent and exacerbated oral symptoms.

    DOI: 10.3390/ijerph18116054

    PubMed

    researchmap

  • Prospective Longitudinal Changes in the Periodontal Inflamed Surface Area Following Active Periodontal Treatment for Chronic Periodontitis. 国際誌

    Yoshiaki Nomura, Toshiya Morozumi, Atsushi Saito, Atsutoshi Yoshimura, Erika Kakuta, Fumihiko Suzuki, Fusanori Nishimura, Hideki Takai, Hiroaki Kobayashi, Kazuyuki Noguchi, Keiso Takahashi, Koichi Tabeta, Makoto Umeda, Masato Minabe, Mitsuo Fukuda, Naoyuki Sugano, Nobuhiro Hanada, Nobuo Yoshinari, Satoshi Sekino, Shogo Takashiba, Soh Sato, Toshiaki Nakamura, Tsutomu Sugaya, Yohei Nakayama, Yorimasa Ogata, Yukihiro Numabe, Taneaki Nakagawa

    Journal of clinical medicine   10 ( 6 )   2021年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Periodontal disease is a chronic inflammatory disease of the periodontal tissue. The periodontal inflamed surface area (PISA) is a proposed index for quantifying the inflammatory burden resulting from periodontitis lesions. This study aimed to investigate longitudinal changes in the periodontal status as evaluated by the PISA following the active periodontal treatment. To elucidate the prognostic factors of PISA, mixed-effect modeling was performed for clinical parameters, tooth-type, and levels of periodontal pathogens as independent variables. One-hundred-twenty-five patients with chronic periodontitis who completed the active periodontal treatment were followed-up for 24 months, with evaluations conducted at 6-month intervals. Five-times repeated measures of mean PISA values were 130+/-173, 161+/-276, 184+/-320, 175+/-417, and 209+/-469 mm2. Changes in clinical parameters and salivary and subgingival periodontal pathogens were analyzed by mixed-effect modeling. Plaque index, clinical attachment level, and salivary levels of Porphyromonas gingivalis were associated with changes in PISA at the patient- and tooth-level. Subgingival levels of P. gingivalis and Prevotella intermedia were associated with changes in PISA at the sample site. For most patients, changes in PISA were within 10% of baseline during the 24-month follow-up. However, an increase in the number of bleeding sites in a tooth with a deep periodontal pocket increased the PISA value exponentially.

    DOI: 10.3390/jcm10061165

    PubMed

    researchmap

  • Estimation of the Periodontal Inflamed Surface Area by Simple Oral Examination. 国際誌

    Yoshiaki Nomura, Toshiya Morozumi, Yukihiro Numabe, Yorimasa Ogata, Yohei Nakayama, Tsutomu Sugaya, Toshiaki Nakamura, Soh Sato, Shogo Takashiba, Satoshi Sekino, Nobuo Yoshinari, Nobuhiro Hanada, Naoyuki Sugano, Mitsuo Fukuda, Masato Minabe, Makoto Umeda, Koichi Tabeta, Keiso Takahashi, Kazuyuki Noguchi, Hiroaki Kobayashi, Hideki Takai, Fusanori Nishimura, Fumihiko Suzuki, Erika Kakuta, Atsutoshi Yoshimura, Atsushi Saito, Taneaki Nakagawa

    Journal of clinical medicine   10 ( 4 )   2021年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The periodontal inflamed surface area (PISA) is a useful index for clinical and epidemiological assessments, since it can represent the inflammation status of patients in one contentious variable. However, calculation of the PISA is difficult, requiring six point probing depth measurements with or without bleeding on probing on 28 teeth, followed by data input in a calculation program. More simple methods are essential for screening periodontal disease or in epidemiological studies. In this study, we tried to establish a convenient partial examination method to estimate PISA. Cross-sectional data of 254 subjects who completed active periodontal therapy were analyzed. Teeth that represent the PISA value were selected by an item response theory approach. The maxillary second molar, first premolar, and lateral incisor and the mandibular second molar and lateral incisor were selected. The sum of the PISAs of these teeth was significantly correlated with the patient's PISA (R2 = 0.938). More simply, the sum of the maximum values of probing pocket depth with bleeding for these teeth were also significantly correlated with the patient's PISA (R2 = 0.6457). The simple model presented in this study may be useful to estimate PISA.

    DOI: 10.3390/jcm10040723

    PubMed

    researchmap

  • Microbiome composition comparison in oral and atherosclerotic plaque from patients with and without periodontitis.

    Daichi Isoshima, Keisuke Yamashiro, Kazuyuki Matsunaga, Makoto Taniguchi, Takehiro Matsubara, Shuta Tomida, Shinzo Ota, Michiyoshi Sato, Yutaka Shimoe, Tatsuo Kohriyama, Zulema Arias, Kazuhiro Omori, Tadashi Yamamoto, Shogo Takashiba

    Odontology   109 ( 1 )   239 - 249   2021年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    There is no conclusive evidence regarding a causal relationship between periodontitis and atherosclerosis. In this study, we examined the microbiome in the oral cavity and atheromatous plaques from atherosclerosis patients with or without periodontitis to investigate the role of oral bacteria in the formation of atheromatous plaques. We chose four patients with and without periodontitis, who had undergone carotid endarterectomy. Bacterial samples were extracted from the tongue surface, from periodontal pocket (during the oral examination), and from the atheromatous plaques (APs). We investigated the general and oral conditions from each patient and performed next-generation sequencing (NGS) analysis for all bacterial samples. There were no significant differences between both groups concerning general conditions. However, the microbiome patterns of the gingival pocket showed differences depending on the absence or presence of periodontitis, while those of the tongue surface were relatively similar. The microbiome pattern of the atheromatous plaques was entirely different from that on the tongue surface and gingival pocket, and oral bacteria were seldom detected. However, the microbiome pattern in atheromatous plaques was different in the presence or absence of periodontitis. These results suggested that oral bacteria did not affect the formation of atheromatous plaques directly.

    DOI: 10.1007/s10266-020-00524-w

    PubMed

    researchmap

  • The Fungal Metabolite (+)-Terrein Abrogates Ovariectomy-Induced Bone Loss and Receptor Activator of Nuclear Factor-κB Ligand-Induced Osteoclastogenesis by Suppressing Protein Kinase-C α/βII Phosphorylation. 国際誌

    Kyosuke Sakaida, Kazuhiro Omori, Masaaki Nakayama, Hiroki Mandai, Saki Nakagawa, Hidefumi Sako, Chiaki Kamei, Satoshi Yamamoto, Hiroya Kobayashi, Satoki Ishii, Mitsuaki Ono, Soichiro Ibaragi, Keisuke Yamashiro, Tadashi Yamamoto, Seiji Suga, Shogo Takashiba

    Frontiers in pharmacology   12   674366 - 674366   2021年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Osteoporosis is a common disease characterized by a systemic impairment of bone mass and microarchitecture that results in fragility fractures. Severe bone loss due to osteoporosis triggers pathological fractures and consequently decreases the daily life activity and quality of life. Therefore, prevention of osteoporosis has become an important issue to be addressed. We have reported that the fungal secondary metabolite (+)-terrein (TER), a natural compound derived from Aspergillus terreus, has shown receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation by suppressing nuclear factor of activated T-cell 1 (NFATc1) expression, a master regulator of osteoclastogenesis. TER has been shown to possess extensive biological and pharmacological benefits; however, its effects on bone metabolism remain unclear. In this study, we investigated the effects of TER on the femoral bone metabolism using a mouse-ovariectomized osteoporosis model (OVX mice) and then on RANKL signal transduction using mouse bone marrow macrophages (mBMMs). In vivo administration of TER significantly improved bone density, bone mass, and trabecular number in OVX mice (p < 0.01). In addition, TER suppressed TRAP and cathepsin-K expression in the tissue sections of OVX mice (p < 0.01). In an in vitro study, TER suppressed RANKL-induced phosphorylation of PKCα/βII, which is involved in the expression of NFATc1 (p < 0.05). The PKC inhibitor, GF109203X, also inhibited RANKL-induced osteoclastogenesis in mBMMs as well as TER. In addition, TER suppressed the expression of osteoclastogenesis-related genes, such as Ocstamp, Dcstamp, Calcr, Atp6v0d2, Oscar, and Itgb3 (p < 0.01). These results provide promising evidence for the potential therapeutic application of TER as a novel treatment compound against osteoporosis.

    DOI: 10.3389/fphar.2021.674366

    PubMed

    researchmap

  • Optimal Examination Sites for Periodontal Disease Evaluation: Applying the Item Response Theory Graded Response Model. 国際誌

    Yoshiaki Nomura, Toshiya Morozumi, Mitsuo Fukuda, Nobuhiro Hanada, Erika Kakuta, Hiroaki Kobayashi, Masato Minabe, Toshiaki Nakamura, Yohei Nakayama, Fusanori Nishimura, Kazuyuki Noguchi, Yukihiro Numabe, Yorimasa Ogata, Atsushi Saito, Soh Sato, Satoshi Sekino, Naoyuki Sugano, Tsutomu Sugaya, Fumihiko Suzuki, Keiso Takahashi, Hideki Takai, Shogo Takashiba, Makoto Umeda, Hiromasa Yoshie, Atsutoshi Yoshimura, Nobuo Yoshinari, Taneaki Nakagawa

    Journal of clinical medicine   9 ( 11 )   2020年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Periodontal examination data have a complex structure. For epidemiological studies, mass screenings, and public health use, a simple index that represents the periodontal condition is necessary. Periodontal indices for partial examination of selected teeth have been developed. However, the selected teeth vary between indices, and a justification for the selection of examination teeth has not been presented. We applied a graded response model based on the item response theory to select optimal examination teeth and sites that represent periodontal conditions. Data were obtained from 254 patients who participated in a multicenter follow-up study. Baseline data were obtained from initial follow-up. Optimal examination sites were selected using item information calculated by graded response modeling. Twelve sites-maxillary 2nd premolar (palatal-medial), 1st premolar (palatal-distal), canine (palatal-medial), lateral incisor (palatal-central), central incisor (palatal-distal) and mandibular 1st premolar (lingual, medial)-were selected. Mean values for clinical attachment level, probing pocket depth, and bleeding on probing by full mouth examinations were used for objective variables. Measuring the clinical parameters of these sites can predict the results of full mouth examination. For calculating the periodontal index by partial oral examination, a justification for the selection of examination sites is essential. This study presents an evidence-based partial examination methodology and its modeling.

    DOI: 10.3390/jcm9113754

    PubMed

    researchmap

  • Follistatin expressed in mechanically-damaged salivary glands of male mice induces proliferation of CD49f+ cells. 国際誌

    A Ikeda, T Yamamoto, J Mineshiba, S Takashiba

    Scientific reports   10 ( 1 )   19959 - 19959   2020年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Salivary glands (SGs) are very important for maintaining the physiological functions of the mouth. When SGs regenerate and repair from various damages, including mechanical, radiological, and immune diseases, acinar and granular duct cells originate from intercalated duct cells. However, the recovery is often insufficient because of SGs' limited self-repair function. Furthermore, the precise repair mechanism has been unclear. Here, we focused on CD49f, one of the putative stem cell markers, and characterized CD49f positive cells (CD49f+ cells) isolated from male murine SGs. CD49f+ cells possess self-renewal ability and express epithelial and pluripotent markers. Compared to CD49f negative cells, freshly isolated CD49f+ cells highly expressed inhibin beta A and beta B, which are components of activin that has anti-proliferative effects. Notably, an inhibitor of activin, follistatin was expressed in mechanically-damaged SGs, meanwhile no follistatin was expressed in normal SGs in vivo. Moreover, sub-cultured CD49f+ cells highly expressed both Follistatin and a series of proliferative genes, expressions of which were decreased by Follistatin siRNA. These findings indicated that the molecular interaction between activin and follistatin may induce CD49f+ cells proliferation in the regeneration and repair of mouse SGs.

    DOI: 10.1038/s41598-020-77004-2

    PubMed

    researchmap

  • Isolation and identification of the antimicrobial substance included in tempeh using Rhizopus stolonifer NBRC 30816 for fermentation. 国際誌

    Masahiro Ito, Takashi Ito, Hideyuki Aoki, Koshi Nishioka, Tsugumi Shiokawa, Hiroko Tada, Yuki Takeuchi, Nobuyuki Takeyasu, Tadashi Yamamoto, Shogo Takashiba

    International journal of food microbiology   325   108645 - 108645   2020年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In this study, we focus on the antimicrobial properties of tempeh, a soybean fermented food, against oral bacteria. Tempeh showed antimicrobial activity against dental caries pathogenic bacterium Streptococcus mutans at a final concentration of 1 mg/mL. An antimicrobial substance contained in tempeh was present in the 100 kDa or greater fraction generated by ultrafiltration, but it was found not to be proteinaceous by native-PAGE, SDS-PAGE and protein degradation tests. Next, when the fraction was purified with an ODS column, the 80% and 100% methanol eluates showed antimicrobial activity against S. mutans. The 100% methanol eluate was further subjected to a 2nd column purification, and isolation of the target was confirmed by HPLC. When the isolated material was analyzed by ESI-MS, the m/z was 279.234. Further analysis by Raman spectroscopy revealed a peak similar to linoleic acid. This substance also possessed antimicrobial properties equivalent to linoleic acid.

    DOI: 10.1016/j.ijfoodmicro.2020.108645

    Scopus

    PubMed

    researchmap

  • Evaluation of the simulator with automatic irrigation control system designed for countermeasures of internal contamination in dental unit water lines. 国際誌

    Keisuke Okubo, Takashi Ito, Kentaro Okamoto, Ichiro Yamamoto, Hajime Mizutani, Yusuke Kawata, Yasuyoshi Shiota, Masahiro Ito, Shin Nakamura, Masako Tai, Tadashi Yamamoto, Shogo Takashiba

    Heliyon   6 ( 6 )   e04132   2020年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The prevention of nosocomial infections is an imperative task. The dental chair unit (DCU) is an indispensable device used in dental treatment. However, it is known that the dental unit water line (DUWL) can become contaminated with biofilm, consisting mainly of heterotrophic bacteria (HB). Recently, the International Organization for Standardization specified the methods for testing DUWL contamination management. On these grounds, a simulator reproducing DUWL was prepared to standardize the examination method of the DUWL contamination. Objectives: To evaluate the reproducibility of the DUWL simulator, monitor the DUWL contamination states, and test the efficacy of a commercial decontaminant for DUWL. Methods: The DUWL simulator was assembled by a DCU manufacturing company. The simulator's DUWL was filled with tap water (TW), and left for approximately one year. Neutral electrolyzed water (NEW) was used as a decontaminant for DUWL. Both TW and NEW were passed through DUWL in a timely manner simulating daily dental treatment. Water was sampled from the air turbine hand piece weekly for 4 weeks and used for HB culture. Contamination status was evaluated by measuring bacterial adenosine triphosphate release and by culturing on Reasoner's 2A medium. Results: The DUWL released contaminated water had a bacterial count of over 6 × 104 cfu/mL. After passing NEW through DUWL for 1 week, the count drastically decreased to its basal level and remained steady for 4 weeks. However, TW showed no effect on DUWL decontamination throughout the examination periods. Conclusions: The DUWL simulator could be useful to examine the efficacy of the decontaminant for DUWL and development of new methods in DUWL contamination management.

    DOI: 10.1016/j.heliyon.2020.e04132

    PubMed

    researchmap

  • The fungal metabolite (+)-terrein abrogates osteoclast differentiation via suppression of the RANKL signaling pathway through NFATc1. 査読 国際誌

    Saki Nakagawa, Kazuhiro Omori, Masaaki Nakayama, Hiroki Mandai, Satoshi Yamamoto, Hiroya Kobayashi, Hidefumi Sako, Kyosuke Sakaida, Hiroshi Yoshimura, Satoki Ishii, Soichiro Ibaragi, Kimito Hirai, Keisuke Yamashiro, Tadashi Yamamoto, Seiji Suga, Shogo Takashiba

    International immunopharmacology   83   106429 - 106429   2020年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Pathophysiological bone resorption is commonly associated with periodontal disease and involves the excessive resorption of bone matrix by activated osteoclasts. Receptor activator of nuclear factor (NF)-κB ligand (RANKL) signaling pathways have been proposed as targets for inhibiting osteoclast differentiation and bone resorption. The fungal secondary metabolite (+)-terrein is a natural compound derived from Aspergillus terreus that has previously shown anti-interleukin-6 properties related to inflammatory bone resorption. However, its effects and molecular mechanism of action on osteoclastogenesis and bone resorption remain unclear. In the present study, we showed that 10 µM synthetic (+)-terrein inhibited RANKL-induced osteoclast formation and bone resorption in a dose-dependent manner and without cytotoxicity. RANKL-induced messenger RNA expression of osteoclast-specific markers including nuclear factor of activated T-cells cytoplasmic 1 (NFATc1), the master regulator of osteoclastogenesis, cathepsin K, tartrate-resistant acid phosphatase (Trap) was completely inhibited by synthetic (+)-terrein treatment. Furthermore, synthetic (+)-terrein decreased RANKL-induced NFATc1 protein expression. This study revealed that synthetic (+)-terrein attenuated osteoclast formation and bone resorption by mediating RANKL signaling pathways, especially NFATc1, and indicated the potential effect of (+)-terrein on inflammatory bone resorption including periodontal disease.

    DOI: 10.1016/j.intimp.2020.106429

    PubMed

    researchmap

  • Efficacy and safety of PERIOdontal treatment versus usual care for Nonalcoholic liver disease: protocol of the PERION multicenter, two-arm, open-label, randomized trial. 国際誌

    Yohei Kamata, Takaomi Kessoku, Tomoko Shimizu, Takashi Kobayashi, Takeo Kurihashi, Satsuki Sato, Syotaro Kuraji, Norio Aoyama, Tomoyuki Iwasaki, Shogo Takashiba, Nobushiro Hamada, Toshiro Kodama, Toshiyuki Tamura, Satoshi Ino, Takuma Higurashi, Masataka Taguri, Takeharu Yamanaka, Masato Yoneda, Haruki Usuda, Koichiro Wada, Atsushi Nakajima, Masato Minabe

    Trials   21 ( 1 )   291 - 291   2020年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: We report the first protocol for a multicenter, randomized comparison study to compare the efficacies of periodontal scaling and root-planing treatment against that of tooth-brushing treatment for nonalcoholic fatty liver disease (NAFLD) (PERION: PERIOdontal treatment for NAFLD). Nonalcoholic steatohepatitis (NASH) is an advanced form of NAFLD, which can progress to cirrhosis and hepatocellular carcinoma. Increased endotoxemia is associated with the progression of NAFLD. Periodontal bacteria possess endotoxins; Porphyromonas gingivalis is well-known as a major pathogenic bacterium in periodontitis, and serum antibody levels for P. gingivalis are high in patients with periodontitis. Several reports have indicated that P. gingivalis is related to NAFLD. This study aims to investigate the effect of periodontal treatment for liver damage, P. gingivalis infection, and endotoxemia on patients with NAFLD. METHODS: We will include adult patients (20-85 years old) with NAFLD, alanine aminotransferase (ALT) ≥ 40 IU/L, and equivalent steatosis grade ≥ 1 (target sample size, n = 40 patients; planned number of patients with outcome data, n = 32). Participants will be randomly assigned to one of two groups: a scaling and root-planing group or tooth-brushing as the usual group. The primary outcome will be the change in ALT levels from baseline to 12 weeks; the key secondary outcome will be the change in the serum immunoglobulin G (IgG) antibody titer for P. gingivalis at 12 weeks. DISCUSSION: This study should determine whether periodontal treatment decreases liver damage, P. gingivalis infection, and endotoxemia in patients with NAFLD. TRIAL REGISTRATION: University Hospital Medical Information Network (UMIN) Clinical Trials Registry, ID: UMIN000022079.

    DOI: 10.1186/s13063-020-4201-y

    PubMed

    researchmap

  • Antimicrobial and antibiofilm effects of abietic acid on cariogenic Streptococcus mutans.

    Yuki Ito, Takashi Ito, Keisuke Yamashiro, Fumi Mineshiba, Kimito Hirai, Kazuhiro Omori, Tadashi Yamamoto, Shogo Takashiba

    Odontology   108 ( 1 )   57 - 65   2020年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Dental caries is a type of oral microbiome dysbiosis and biofilm infection that affects oral and systemic conditions. For healthy life expectancy, natural bacteriostatic products are ideal for daily and lifetime use as anti-oral infection agents. This study aimed to evaluate the inhibitory effects of abietic acid, a diterpene derived from pine rosin, on the in vitro growth of cariogenic bacterial species, Streptococcus mutans. The effective minimum inhibitory concentration of abietic acid was determined through observation of S. mutans growth, acidification, and biofilm formation. The inhibitory effects of abietic acid on the bacterial membrane were investigated through the use of in situ viability analysis and scanning electron microscopic analysis. Cytotoxicity of abietic acid was also examined in the context of several human cell lines using tetrazolium reduction assay. Abietic acid was found to inhibit key bacterial growth hallmarks such as colony forming ability, adenosine triphosphate activity (both planktonic and biofilm), acid production, and biofilm formation. Abietic acid was identified as bacteriostatic, and this compound caused minimal damage to the bacterial membrane. This action was different from that of povidone-iodine or cetylpyridinium chloride. Additionally, abietic acid was significantly less cytotoxic compared to povidone-iodine, and it exerted lower toxicity towards epithelial cells and fibroblasts compared to that against monocytic cells. These data suggest that abietic acid may prove useful as an antibacterial and antibiofilm agent for controlling S. mutans infection.

    DOI: 10.1007/s10266-019-00456-0

    PubMed

    researchmap

  • Graphene oxide: A new direction in dentistry

    Nizami, M.Z.I., Takashiba, S., Nishina, Y.

    Applied Materials Today   19   2020年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.apmt.2020.100576

    Scopus

    researchmap

  • Tailoring the interaction between graphene oxide and antibacterial pyridinium salts by terminal functional groups

    Fujii, R., Okubo, K., Takashiba, S., Bianco, A., Nishina, Y.

    Carbon   160   2020年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.carbon.2019.11.094

    Scopus

    researchmap

  • Identification and Modification of Porphyromonas gingivalis Cysteine Protease, Gingipain, Ideal for Screening Periodontitis. 国際誌

    Kimito Hirai, Tomoko Yamaguchi-Tomikawa, Toru Eguchi, Hiroshi Maeda, Shogo Takashiba

    Frontiers in immunology   11   1017 - 1017   2020年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Chronic periodontitis is an inflammatory disease caused by the formation of oral microbial biofilms. Periodontitis is associated with general health and not only oral diseases. Porphyromonas gingivalis is a well-known keystone pathogen for periodontitis and is associated with several systemic diseases, such as diabetes mellitus and Alzheimer's disease. We previously developed a system for screening periodontitis using P. gingivalis-specific serum immunoglobulin G (IgG) in an enzyme-linked immunosorbent assay with a sensitivity of 0.774 and a specificity of 0.586 and an area under the receiver operating characteristic curve of 0.708. However, the antigens elicited non-specific responses, since they were obtained from whole extracts of sonicated cultured bacteria. The purpose of this study was to identify antigens ideal for a sensitive and specific serum test. We identified the specific antigens using immunoaffinity columns immobilized with IgG antibodies from periodontitis patients. Liquid chromatography-tandem mass spectrometry identified 29 antigens from the elutes. Recombinant proteins for these candidates were synthesized using the wheat germ cell-free translation system and screened by dot blot analysis with serum from the columns. Three of the 16 candidates that reacted showed strongest affinities upon dot blot analysis; they included outer membrane protein 28, cysteine proteases, lysine gingipain Kgp, and arginine gingipain RgpA. Outer membrane protein 28 was not suitable for screening P. gingivalis infection because of its high false-negative rates. Kgp and RgpA were unstable antigens since they underwent self-digestion. They were made stable by substituting the active cysteine residues in Kgp and RgpA with alanine using site-directed mutagenesis. Using the modified antigens, we demonstrated that the patient serum IgG level against RgpA was the highest among all the antigens expressed in P. gingivalis. Moreover, the N-terminus of recombinant RgpA was excellent in differentiating between diseased and non-diseased states (with sensitivity of 0.85, specificity of 0.9, and area under the curve of 0.915). Although dot blot analysis was the only experiment used, the N-terminus of RgpA is an excellent antigen to immunologically test for P. gingivalis infection, especially for estimating the risks for periodontitis-associated systemic diseases. In conclusion, we have developed a P. gingivalis antigen for screening periodontitis.

    DOI: 10.3389/fimmu.2020.01017

    PubMed

    researchmap

  • High Mobility Group Box 1 Expression in Oral Inflammation and Regeneration. 国際誌

    Keisuke Yamashiro, Hidetaka Ideguchi, Hiroaki Aoyagi, Chiaki Yoshihara-Hirata, Anna Hirai, Risa Suzuki-Kyoshima, Yao Zhang, Hidenori Wake, Masahiro Nishibori, Tadashi Yamamoto, Shogo Takashiba

    Frontiers in immunology   11   1461 - 1461   2020年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    High mobility group box 1 (HMGB1) is a non-histone DNA-binding protein of about 30 kDa. It is released from a variety of cells into the extracellular milieu in response to inflammatory stimuli and acts on specific cell-surface receptors, such as receptors for advanced glycation end-products (RAGE), Toll-like receptor (TLR)2, TLR4, with or without forming a complex with other molecules. HMGB1 mediates various mechanisms such as inflammation, cell migration, proliferation, and differentiation. On the other hand, HMGB1 enhances chemotaxis acting through the C-X-C motif chemokine ligand (CXCL)12/C-X-C chemokine receptor (CXCR)4 axis and is involved in regeneration. In the oral cavity, high levels of HMGB1 have been detected in the gingival tissue from periodontitis and peri-implantitis patients, and it has been shown that secreted HMGB1 induces pro-inflammatory cytokine expression, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α, which prolong inflammation. In contrast, wound healing after tooth extraction or titanium dental implant osseointegration requires an initial acute inflammation, which is regulated by secreted HMGB1. This indicates that secreted HMGB1 regulates angiogenesis and bone remodeling by osteoclast and osteoblast activation and promotes bone healing in oral tissue repair. Therefore, HMGB1 can prolong inflammation in the periodontal tissue and, conversely, can regenerate or repair damaged tissues in the oral cavity. In this review, we highlight the role of HMGB1 in the oral cavity by comparing its function and regulation with its function in other diseases. We also discuss the necessity for further studies in this field to provide more specific scientific evidence for dentistry.

    DOI: 10.3389/fimmu.2020.01461

    PubMed

    researchmap

  • Acute Prevertebral Abscesses Caused by Bacterial-infected Traumatic Tooth Fractures.

    Kazuyuki Matsunaga, Makoto Takemaru, Keisuke Yamashiro, Chiaki Yoshihara-Hirata, Ken Inohara, Yutaka Shimoe, Akio Tanaka, Masaru Kuriyama, Shogo Takashiba

    Acta medica Okayama   73 ( 5 )   449 - 456   2019年10月

     詳細を見る

    記述言語:英語  

    We report a case of acute prevertebral abscess caused by traumatic tooth fractures in a 77-year-old Japanese man. After being transferred to our hospital the patient was initially diagnosed with a neck hematoma; however, blood culture showed Streptococcus parasanguinis, an oral bacterium, and an MRI examination suggested prevertebral abscesses. Tooth fractures, severe periodontitis, and peri-implantitis with Streptococcus parasanguinis were observed. Antibiotics were administered and fractured teeth were extracted. The patient's condition then gradually improved. We concluded that bacteremia caused by traumatic tooth fractures induced the acute prevertebral abscesses.

    DOI: 10.18926/AMO/57376

    PubMed

    researchmap

  • Construction and characterization of a PGN_0297 mutant of Porphyromonas gingivalis: evidence of the contribution of PGN_0297 to gingipain activity. 査読

    Ono S, Nakayama M, Tachibana M, Shahriar ASM, Heling W, Takashiba S, Ohara N

    Acta Medica Okayama   73 ( 4 )   315 - 323   2019年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.18926/AMO/56933

    PubMed

    researchmap

  • Effectiveness and safety of low-concentrated ozonized water for the reduction of contamination in dental unit water lines. 国際誌

    Keisuke Okubo, Takashi Ito, Yasuyoshi Shiota, Yusuke Kawata, Tadashi Yamamoto, Shogo Takashiba

    Heliyon   5 ( 8 )   e02306   2019年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Contamination of dental unit waterlines (DUWL) with heterotrophic bacteria can cause problems in immune compromised patients (aged, tumor and organ transplantation-patients). We focused on the use of low-concentrated ozonized water (OZW) as the biofilm formation restraint system for DUWL. Here, we examined the effects of low-concentrated OZW on the growth of bacteria and related biofilm formation and harmfulness to dental unit components (DUCs) in vitro. Objectives: To evaluate the bactericidal effects of OZW on biofilms in DUWL and DUC in vitro. Methods: Low-concentrated OZW (0.4 mg/L) was generated using an OZS-PTDX generator. Heterotrophic bacterial biofilms in old DUWL tubes and Candia albicans solution (control microbe) were treated with OZW for 1 h with gentle agitation before static culturing for 96 h in Reasoner's 2A liquid media. The control solutions were 0.1% cetylpyridinium chloride (CPC), chlorinated tap water (TW), and phosphate-buffered saline (PBS). Adenosine triphosphate (ATP) amounts of the microbes were measured and the biofilms of these microbes were observed using scanning electron microscopy (SEM). Moreover, surfaces of DUC soaked in OZW and TW were observed by SEM. Results: The OZW reduced ATP levels in microbes to 50% compared to TW and PBS treatment, although CPC reduced it below detection limits. SEM observation revealed deformation of microbes cultured with OZW, whereas no changes were seen on DUC surfaces. Conclusions: Low-concentrated OZW is bactericidal against heterotrophic bacteria biofilms and it is not harmful to DUC, suggesting that it might be useful in preventing DUWL contamination.

    DOI: 10.1016/j.heliyon.2019.e02306

    PubMed

    researchmap

  • Multidisciplinary clinical approach by sharing oral examination information to treat a diabetes patient with dysgeusia. 国際誌

    Kazuyuki Matsunaga, Yasuko Yoshida, Makoto Takemaru, Keisuke Yamashiro, Ikuko Monden, Ken Inohara, Saki Nakagawa, Eriko Maeda, Kanako Nakahama, Tatsuo Kohriyama, Shogo Takashiba

    Clinical case reports   7 ( 5 )   877 - 880   2019年5月

     詳細を見る

    記述言語:英語  

    Taste alteration is one of the complications of severe diabetes. It is important in diabetes treatment to assess taste alteration and perform dietary counseling, therapeutic exercise, and oral care. In this case, multidisciplinary clinical approach by medical staff was successful for a severely diabetic patient with dysgeusia.

    DOI: 10.1002/ccr3.2111

    PubMed

    researchmap

  • Photoelectrochemical properties of a well-structured 1.3 nm-thick pn junction crystal. 国際誌

    Keisuke Awaya, Akihide Takashiba, Takaaki Taniguchi, Michio Koinuma, Tatsumi Ishihara, Shintaro Ida

    Chemical communications (Cambridge, England)   55 ( 31 )   4586 - 4588   2019年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A 1.3 nm-thick nickel hydroxide (p-type, 0.5 nm)/titania (n-type, 0.8 nm) pn junction prepared by lamination of nanosheets improved the onset potential for photoelectrochemical oxidation and increased the photooxidation current, indicating that ultrathin pn junctions suppress the recombination of photo-generated carriers.

    DOI: 10.1039/c9cc01039d

    PubMed

    researchmap

  • Induction of migration of periodontal ligament cells by selective regulation of integrin subunits. 国際誌

    Mari Kawamura, Tadashi Yamamoto, Keisuke Yamashiro, Shinsuke Kochi, Chiaki Yoshihara-Hirata, Hidetaka Ideguchi, Hiroaki Aoyagi, Kazuhiro Omori, Shogo Takashiba

    Journal of cellular and molecular medicine   23 ( 2 )   1211 - 1223   2019年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The recruitment of tissue-resident stem cells is important for wound regeneration. Periodontal ligament cells (PDL cells) are heterogeneous cell populations with stemness features that migrate into wound sites to regenerate periodontal fibres and neighbouring hard tissues. Cell migration is regulated by the local microenvironment, coordinated by growth factors and the extracellular matrix (ECM). Integrin-mediated cell adhesion to the ECM provides essential signals for migration. We hypothesized that PDL cell migration could be enhanced by selective expression of integrins. The migration of primary cultured PDL cells was induced by platelet-derived growth factor-BB (PDGF-BB). The effects of blocking specific integrins on migration and ECM adhesion were investigated based on the integrin expression profiles observed during migration. Up-regulation of integrins α3, α5, and fibronectin was identified at distinct localizations in migrating PDL cells. Treatment with anti-integrin α5 antibodies inhibited PDL cell migration. Treatment with anti-integrin α3, α3-blocking peptide, and α3 siRNA significantly enhanced cell migration, comparable to treatment with PDGF-BB. Furthermore, integrin α3 inhibition preferentially enhanced adhesion to fibronectin via integrin α5. These findings indicate that PDL cell migration is reciprocally regulated by integrin α3-mediated inhibition and α5-mediated promotion. Thus, targeting integrin expression is a possible therapeutic strategy for periodontal regeneration.

    DOI: 10.1111/jcmm.14023

    PubMed

    researchmap

  • Involvement of PM2.5-bound protein and metals in PM2.5-induced allergic airway inflammation in mice

    Keiki Ogino, Kenjiro Nagaoka, Tatsuo Ito, Kei Takemoto, Tomoaki Okuda, Shoji F. Nakayama, Noriyoshi Ogino, Yuka Seki, Hiroki Hamada, Shogo Takashiba, Yoshihisa Fujikura

    Inhalation Toxicology   30 ( 13-14 )   498 - 508   2018年12月

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    Background: The aim of this study was to investigate the protein and trace element components of PM2.5 and their contribution to the allergic airway inflammation in BALB/c mice. Methods: PM2.5, treated at high temperature and with a strong acid to hydrolyze any protein content and remove trace elements, was administered to BALB/c mice. Allergic airway inflammation was compared between the three groups (saline, pure PM2.5 and treated PM2.5) by evaluating airway hyperresponsiveness (AHR), bronchoalveolar lavage fluid (BALF) cells, serum IgE, the mRNA of various cytokine (IL-4, IL-5, IL-13, eotaxin-1 and CXCL3), mucus protein mRNA (MUC5ac and MUC5b) and the filtration of inflammatory cells in the lung. Results: The treatment of PM2.5 with a strong acid at a high temperature attenuated AHR, eosinophil percentage in BALF, mRNA levels of IL-13 and CXCL3 and peribronchial inflammation. On the contrary, the percentage of neutrophils in BALF, mRNA expression of MIP2α, EGFR, Nrf2, and TLR4 and 4-OH-2-nonenal levels in the lung was increased. Moreover, the treatment of the PM2.5 reduced PM2.5-bound proteins as well as the percentages of the trace elements in PM2.5 in the order Zn > Cu > Pb > P > S > Mn > Fe > Ca > Ni, whereas the percentage of C, Si and Cl increased. Conclusions: PM2.5 collected by of the cyclone system induced allergic airway inflammation in mice. PM2.5-bound proteins and acid-soluble metals may be involved in the pathogenesis of PM2.5-induced allergic airway inflammation.

    DOI: 10.1080/08958378.2018.1561769

    Scopus

    PubMed

    researchmap

  • Fungal metabolite (+)-terrein suppresses IL-6/sIL-6R-induced CSF1 secretion by inhibiting JAK1 phosphorylation in human gingival fibroblasts. 査読 国際誌

    Satoshi Yamamoto, Kazuhiro Omori, Hiroki Mandai, Masaaki Nakayama, Saki Nakagawa, Hiroya Kobayashi, Tadashi Kunimine, Hiroshi Yoshimura, Kyosuke Sakaida, Hidefumi Sako, Soichiro Ibaragi, Tadashi Yamamoto, Hiroshi Maeda, Seiji Suga, Shogo Takashiba

    Heliyon   4 ( 11 )   e00979   2018年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Control of bacterial infection-induced inflammatory responses is one of the effective therapeutic approaches of periodontal diseases. Natural products such as lipid mediators and metabolites from microorganisms have been used for decreasing inflammation. We previously reported that (+)-terrein inhibited activation of STAT3 and ERK1/2 in interleukin-6 (IL-6) signaling cascade, leading to prevent vascular endothelial growth factor (VEGF) secretion in human gingival fibroblasts (HGFs). However, little is still known about the role of (+)-terrein on inflammatory responses. In this study, we provided the possibility of novel action that (+)-terrein inhibits activation of Janus-activated kinase 1 (JAK1), which has a central function in IL-6 signaling cascade, and alters expression of mRNAs and proteins induced by IL-6/soluble IL-6 receptor (sIL-6R) stimulation in HGFs. First, we performed PCR array to examine IL-6/sIL-6R-induced mRNA expression, and then expression of mRNA and protein of colony stimulating factor-1 (CSF1) and VEGF were clearly determined by quantitative RT-PCR and ELISA, respectively. Treatment with (+)-terrein suppressed expression of mRNA and protein of CSF1 and VEGF by IL-6/sIL-6R stimulation. Next, to test the effect of (+)-terrein on IL-6/sIL-6R signaling cascade, we demonstrated whether (+)-terrein affects phosphorylation of JAK1 and its downstream proteins, Akt and SHP-2. Western blotting revealed that (+)-terrein inhibited IL-6/sIL-6R-induced phosphorylation of JAK1, Akt, and SHP-2. Therefore, (+)-terrein suppresses IL-6/sIL-6R-induced expression of CSF1 and VEGF via inhibition of JAK1, Akt, and SHP-2. Based on our results, we suggest that (+)-terrein is a candidate compound for anti-inflammatory effect associated with IL-6 signaling.

    DOI: 10.1016/j.heliyon.2018.e00979

    PubMed

    researchmap

  • HMGB1-induced inflammatory response promotes bone healing in murine tooth extraction socket 査読 国際誌

    Hiroaki Aoyagi, Keisuke Yamashiro, Chiaki Hirata-Yoshihara, Hidetaka Ideguchi, Mutsuyo Yamasaki, Mari Kawamura, Tadashi Yamamoto, Shinsuke Kochi, Hidenori Wake, Masahiro Nishibori, Shogo Takashiba

    Journal of Cellular Biochemistry   119 ( 7 )   5481 - 5490   2018年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley-Liss Inc.  

    High mobility group box 1 (HMGB1) is a non-histone DNA-binding protein that is secreted into the extracellular milieu in response to inflammatory stimuli. The secreted HMGB1 has been suggested to mediate various inflammatory diseases. However, it is still unknown whether HMGB1 is involved in a healing process in the tooth extraction socket, the tissue containing gingival epithelium, and alveolar bone that is exposed to oral bacteria. In this study, we constructed a murine tooth extraction model with anti-HMGB1 neutralization antibody administration and observed the inflammatory response and bone healing process in tooth extraction sockets by molecular imaging of myeloperoxidase (MPO) activity, histological analysis, and quantitative RT-PCR. The translocation of HMGB1 from the nucleus to the cytoplasm in gingival epithelial cells and inflammatory cells was inhibited by anti-HMGB1 antibody administration. The MPO activity around the tooth extraction socket was significantly reduced, and the numbers of CD31- and CD68-positive cells were significantly lower in the anti-HMGB1 antibody treatment samples than in the control samples. The TRAP-positive cells, osteocalcin positive cells, and the neoplastic bone area were significantly lower in anti-HMGB1 antibody treatment samples than in control samples. The expression levels of IL-1β and VEGF-A were also decreased in anti-HMGB1 antibody treatment samples compared to that in control samples. Secreted HMGB1 induced initial acute inflammation and inflammatory cells recruitment after tooth extraction. HMGB1 was associated with angiogenesis and bone remodeling by osteoclast and osteoblast activation and promoted bone healing in the tooth extraction socket.

    DOI: 10.1002/jcb.26710

    Web of Science

    Scopus

    PubMed

    researchmap

  • Effects of periodontal treatment on carotid intima-media thickness in patients with lifestyle-related diseases: Japanese prospective multicentre observational study 査読

    Chieko Kudo, Wee Soo Shin, Nobuhiro Sasaki, Kazuo Harai, Kai Kato, Hiroaki Seino, Eiji Goke, Takemasa Fujino, Nobuichi Kuribayashi, Youko Onuki Pearce, Masato Taira, Ryoji Matsushima, Masato Minabe, Shogo Takashiba, Kiyotaka Fujii, Tooru Hanai, Kouya Honda, Yoshichika Horiuchi, Hiroshi Inoue, Kiyoo Ishige, Shinichi Itoh, Sachiho Iwatsuka, Tomoko Kakugawa, Hideto Komai, Chikara Morimoto, Mitsutaka Motoyoshi, Masato Nakamura, Mikiko Niida, Ryuji Numaguchi, Kiyomi Oono, Hidetoshi Sakai, Yasunari Sakomura, Takaaki Shinohara, Yuichi Takakaze, Yukio Tsuruta, Daigaku Uchida, Norihide Ueno, Osamu Yasuda, Periodontitis and Atherosclerosis Project-Tokyo and Chiba Consortiums

    Odontology   106 ( 3 )   349 - 349   2018年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Tokyo  

    Unfortunately, in Table-5 of the original article, the parameter in the 5th row was published incorrectly as “LDL-C (mg/dL)”. The correct parameter should read as “HDL-C (mg/dL)”.

    DOI: 10.1007/s10266-018-0346-5

    Web of Science

    Scopus

    PubMed

    researchmap

  • Effects of periodontal treatment on carotid intima-media thickness in patients with lifestyle-related diseases: Japanese prospective multicentre observational study 査読

    Chieko Kudo, Wee Soo Shin, Nobuhiro Sasaki, Kazuo Harai, Kai Kato, Hiroaki Seino, Eiji Goke, Takemasa Fujino, Nobuichi Kuribayashi, Youko Onuki Pearce, Masato Taira, Ryoji Matsushima, Masato Minabe, Shogo Takashiba, Kiyotaka Fujii, Tooru Hanai, Kouya Honda, Yoshichika Horiuchi, Hiroshi Inoue, Kiyoo Ishige, Shinichi Itoh, Sachiho Iwatsuka, Tomoko Kakugawa, Hideto Komai, Chikara Morimoto, Mitsutaka Motoyoshi, Masato Nakamura, Mikiko Niida, Ryuji Numaguchi, Kiyomi Oono, Hidetoshi Sakai, Yasunari Sakomura, Takaaki Shinohara, Yuichi Takakaze, Yukio Tsuruta, Daigaku Uchida, Norihide Ueno, Osamu Yasuda, Periodontitis and Atherosclerosis Project-Tokyo and Chiba Consortiums

    Odontology   106 ( 3 )   349 - 327   2018年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Tokyo  

    Unfortunately, in Table-5 of the original article, the parameter in the 5th row was published incorrectly as “LDL-C (mg/dL)”. The correct parameter should read as “HDL-C (mg/dL)”.

    DOI: 10.1007/s10266-018-0346-5

    Web of Science

    Scopus

    PubMed

    researchmap

  • Molecular imaging assessment of periodontitis lesions in an experimental mouse model 査読 国際誌

    Hidetaka Ideguchi, Keisuke Yamashiro, Tadashi Yamamoto, Masayuki Shimoe, Shoichi Hongo, Shinsuke Kochi, Chiaki Yoshihara-Hirata, Hiroaki Aoyagi, Mari Kawamura, Shogo Takashiba

    Clinical Oral Investigations   23 ( 2 )   1 - 7   2018年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Verlag  

    Objective: We aimed to evaluate molecular imaging as a novel diagnostic tool for mice periodontitis model induced by ligature and Porphyromonas gingivalis (Pg) inoculation. Materials and methods: Twelve female mice were assigned to the following groups: no treatment as control group (n = 4)
    periodontitis group induced by ligature and Pg as Pg group (n = 4)
    and Pg group treated with glycyrrhizinic acid (GA) as Pg + GA group (n = 4). All mice were administered a myeloperoxidase (MPO) activity-specific luminescent probe and observed using a charge-coupled device camera on day 14. Image analysis on all mice was conducted using software to determine the signal intensity of inflammation. Additionally, histological and radiographic evaluation for periodontal inflammation and bone resorption at the site of periodontitis, and quantitative enzyme-linked immunosorbent assay (ELISA) were conducted on three mice for each group. Each experiment was performed three times. Results: Levels of serum IgG antibody against P. gingivalis were significantly higher in the Pg than in the Pg + GA group. Histological analyses indicated that the number of osteoclasts and neutrophils were significantly lower in the Pg + GA than in the Pg group. Micro-CT image analysis indicated no difference in bone resorption between the Pg and Pg + GA groups. The signal intensity of MPO activity was detected on the complete craniofacial image
    moreover, strong signal intensity was localized specifically at the periodontitis site in the ex vivo palate, with group-wise differences. Conclusions: Molecular imaging analysis based on MPO activity showed high sensitivity of detection of periodontal inflammation in mice. Clinical relevance: Molecular imaging analysis based on MPO activity has potential as a diagnostic tool for periodontitis.

    DOI: 10.1007/s00784-018-2510-2

    Scopus

    PubMed

    researchmap

  • Anti-HMGB1 neutralizing antibody attenuates periodontal inflammation and bone resorption in a murine periodontitis model 査読 国際誌

    Chiaki Yoshihara-Hirata, Keisuke Yamashiro, Tadashi Yamamoto, Hiroaki Aoyagi, Hidetaka Ideguchi, Mari Kawamura, Risa Suzuki, Mitsuaki Ono, Hidenori Wake, Masahiro Nishibori, Shogo Takashiba

    Infection and Immunity   86 ( 5 )   2018年5月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    High mobility group box 1 (HMGB1) is a non-histone DNA-binding protein that is secreted into the extracellular milieu in response to inflammatory stimuli. The secreted HMGB1 mediates various inflammatory diseases, including periodontitis
    however, the underlying mechanisms of HMGB1-induced periodontal inflammation are not completely understood. Here, we examined whether anti-HMGB1 neutralizing antibody inhibits periodontal progression and investigated the molecular pathology of HMGB1 in vitro and in vivo. In vitro analysis indicated that HMGB1, granulocytemacrophage colony-stimulating factor (GM-CSF), and interleukin-1β (IL-1β) were secreted in response to tumor necrosis factor-α (TNF-α) stimuli in human gingival epithelial cells (HGECs) and human monocytic leukemia cells (THP-1) treated with phorbol myristate acetate. Increased levels of GM-CSF and IL-1β were observed in the conditioned media from TNF-α-stimulated HGECs and THP-1 in vitro. Simultaneous stimulation with TNF-α and anti-HMGB1 antibody significantly decreased TNF-α- induced inflammatory cytokine secretion. Experimental periodontitis was induced in mice using Porphyromonas gingivalis-soaked ligatures. The extracellular translocation was confirmed in gingival epithelia in the periodontitis model mice by immunofluorescence analysis. Systemic administration of anti-HMGB1 neutralizing antibody significantly inhibited translocation of HMGB1. The anti-HMGB1 antibody inhibited periodontal inflammation, expression of IL-1β and C-X-C motif chemokine ligand 1 (CXCL1), migration of neutrophils, and bone resorption, shown by bioluminescence imaging of myeloperoxidase activity, quantitative reverse transcription-PCR (RT-PCR), and micro-computed tomography analysis. These findings indicate that HMGB1 is secreted in response to inflammatory stimuli caused by periodontal infection, which is crucial for the initiation of periodontitis, and the anti-HMGB1 antibody attenuates the secretion of a series of inflammatory cytokines, consequently suppressing the progression of periodontitis.

    DOI: 10.1128/IAI.00111-18

    Web of Science

    Scopus

    PubMed

    researchmap

  • Expression of optineurin isolated from rat-injured dental pulp and the effects on inflammatory signals in normal rat kidney cells 査読

    Kyoko Senoo, Keisuke Yamashiro, Tadashi Yamamoto, Fumio Myokai, Mari Kawamura, Shogo Takashiba

    Odontology   106 ( 2 )   223 - 223   2018年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Tokyo  

    In the original publication of the article, one of the author name was published incorrectly as “Keisuke Yamashairo” and correct name should be “Keisuke Yamashiro”.

    DOI: 10.1007/s10266-017-0334-1

    Web of Science

    Scopus

    PubMed

    researchmap

  • Expression of optineurin isolated from rat-injured dental pulp and the effects on inflammatory signals in normal rat kidney cells.

    Kyoko Senoo, Keisuke Yamashiro, Tadashi Yamamoto, Fumio Myokai, Mari Kawamura, Shogo Takashiba

    Odontology   106 ( 2 )   135 - 144   2018年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We previously isolated rat 14.7K-interacting protein-2 (rFIP-2) from the rat-wounded pulp. The protein, homologous to human FIP-2, is known as optineurin and was initially identified as a novel tumor necrosis factor-α (TNF-α)-inducible protein, and more recently, as an autophagy receptor. However, the biological role of optineurin in dental pulp remains elusive. We hypothesized that optineurin has a crucial role in regulating molecular processes during pulp inflammatory responses induced by TNF-α. We examined the kinetics of optineurin expression in pulp inflammation. Optineurin localization and expression were examined using rat pulp fibroblasts. The cells were treated with pharmacological inhibitors for TNF-α-induced inflammatory signals or with hydrogen peroxide as apoptotic stimuli. Stable optineurin-knockdown cells (OPTN-KD cells) were established by transfecting normal rat kidney cells with a vector expressing optineurin-specific small interfering RNA. Cell proliferation and the profiles of cytokines and intracellular signaling molecules were examined using OPTN-KD cells stimulated by TNF-α. Optineurin was localized in the cytoplasm and then translocated into the nucleus upon apoptotic stimuli. Optineurin expression was increased by TNF-α and decreased by a specific inhibitor of c-Jun N-terminal kinase. The OPTN-KD cells secreted smaller amounts of monocyte chemotactic protein-1 (MCP-1) and intracellular MCP-1 mRNA, and cell proliferation was significantly increased. Apoptosis-related signaling molecules were downregulated in OPTN-KD cells. These results demonstrated that optineurin is a crucial molecule mediated by TNF-α, which induces the production of inflammatory factors and apoptosis signaling, suggesting the presence of signaling interactions between optineurin and a transcription factor for MCP-1.

    DOI: 10.1007/s10266-017-0314-5

    PubMed

    researchmap

  • Modulation of microenvironment for controlling the fate of periodontal ligament cells: the role of Rho/ROCK signaling and cytoskeletal dynamics 査読 国際誌

    Tadashi Yamamoto, Yuki Ugawa, Mari Kawamura, Keisuke Yamashiro, Shinsuke Kochi, Hidetaka Ideguchi, Shogo Takashiba

    Journal of Cell Communication and Signaling   12 ( 1 )   369 - 378   2018年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Netherlands  

    Cells behave in a variety of ways when they perceive changes in their microenvironment
    the behavior of cells is guided by their coordinated interactions with growth factors, niche cells, and extracellular matrix (ECM). Modulation of the microenvironment affects the cell morphology and multiple gene expressions. Rho/Rho-associated coiled-coil-containing protein kinase (ROCK) signaling is one of the key regulators of cytoskeletal dynamics and actively and/or passively determines the cell fate, such as proliferation, migration, differentiation, and apoptosis, by reciprocal communication with the microenvironment. During periodontal wound healing, it is important to recruit the residential stem cells into the defect site for regeneration and homeostasis of the periodontal tissue. Periodontal ligament (PDL) cells contain a heterogeneous fibroblast population, including mesenchymal stem cells, and contribute to the reconstruction of tooth-supporting tissues. Therefore, bio-regeneration of PDL cells has been the ultimate goal of periodontal therapy for decades. Recent stem cell researches have shed light on intrinsic ECM properties, providing paradigm shifts in cell fate determination. This review focuses on the role of ROCK activity and the effects of Y-27632, a specific inhibitor of ROCK, in the modulation of ECM-microenvironment. Further, it presents the current understanding of how Rho/ROCK signaling affects the fate determination of stem cells, especially PDL cells. In addition, we have also discussed in detail the underlying mechanisms behind the reciprocal response to the microenvironment.

    DOI: 10.1007/s12079-017-0425-3

    Web of Science

    Scopus

    PubMed

    researchmap

  • The KCNQ1 gene polymorphism as a shared genetic risk for rheumatoid arthritis and chronic periodontitis in Japanese adults: A pilot case-control study. 査読 国際誌

    Kobayashi T, Kido JI, Ishihara Y, Omori K, Ito S, Matsuura T, Bando T, Wada J, Murasawa A, Nakazono K, Mitani A, Takashiba S, Nagata T, Yoshie H

    Journal of periodontology   89 ( 3 )   315 - 324   2018年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1002/JPER.17-0412

    Web of Science

    PubMed

    researchmap

  • Effects of Mechanical Stress on Periodontal Ligament 査読

    Fujita Ayano, Morimatsu Masatoshi, Nishiyama Masayoshi, Takashiba Shogo, Naruse Keiji

    BIOPHYSICAL JOURNAL   114 ( 3 )   143A   2018年2月

  • Effects of Lectins on initial attachment of cariogenic Streptococcus mutans 査読 国際誌

    Takashi Ito, Yasuhiro Yoshida, Yasuyoshi Shiota, Yuki Ito, Tadashi Yamamoto, Shogo Takashiba

    Glycoconjugate Journal   35 ( 1 )   41 - 51   2018年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer New York LLC  

    Oral bacteria initiate biofilm formation by attaching to tooth surfaces via an interaction of a lectin-like bacterial protein with carbohydrate chains on the pellicle. This study aimed to find naturally derived lectins that inhibit the initial attachment of a cariogenic bacterial species, Streptococcus mutans (S. mutans), to carbohydrate chains in saliva in vitro. Seventy kinds of lectins were screened for candidate motifs that inhibit the attachment of S. mutans ATCC 25175 to a saliva-coated culture plate. The inhibitory effect of the lectins on attachment of the S. mutans to the plates was quantified by crystal violet staining, and the biofilm was observed under a scanning electron microscope (SEM). Surface plasmon resonance (SPR) analysis was performed to examine the binding of S. mutans to carbohydrate chains and the binding of candidate lectins to carbohydrate chains, respectively. Moreover, binding assay between the biotinylated-lectins and the saliva components was conducted to measure the lectin binding. Lectins recognizing a salivary carbohydrate chain, Galβ1-3GalNAc, inhibited the binding of S. mutans to the plate. In particular, Agaricus bisporus agglutinin (ABA) markedly inhibited the binding. This inhibition was confirmed by SEM observation. SPR analysis indicated that S. mutans strongly binds to Galβ1-3GalNAc, and ABA binds to Galβ1-3GalNAc. Finally, the biotinylated Galβ1-3GalNAc-binding lectins including ABA demonstrated marked binding to the saliva components. These results suggest that ABA lectin inhibited the attachment of S. mutans to Galβ1-3GalNAc in saliva and ABA can be useful as a potent inhibitor for initial attachment of oral bacteria and biofilm formation.

    DOI: 10.1007/s10719-017-9795-2

    Web of Science

    Scopus

    PubMed

    researchmap

  • IS1598 (IsPg4) distributed to abscess-forming strains of Porphyromonas gingivalis may enhance virulence through upregulation of nrdD-like gene expression 査読 国際誌

    Norihiro Sonoi, Hiroshi Maeda, Toshimitsu Murauchi, Tadashi Yamamoto, Kazuhiro Omori, Susumu Kokeguchi, Koji Naruishi, Shogo Takashiba

    New Microbiologica   41 ( 1 )   52 - 60   2018年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Luigi Ponzio e figlio Editori  

    An insertion sequence, IS1598 (IsPg4) has been found in virulent strains of Porphyromonas gingivalis in a murine abscess model. The present study was performed to investigate the effects of genetic rearrangements by IS1598 on the phenotypic characteristics of the virulent strains. For this purpose, we searched for a common insertion site of IS1598 among the virulent strains. Through cloning and database search, a common insertion site was identified beside an nrdD-like gene in the virulent FDC 381, W83 and W50 strains. In this region, predicted promoters of the nrdD-like gene and IS1598 are located in tandem, and accumulation of nrdD-like gene mRNA was 5-fold higher in virulent strains (W83, W50, FDC 381) than avirulent strains (ATCC33277, SU63, SUNY1021, ESO59 without IS1598). The role of the nrdD-like gene in virulence of P. gingivalis was investigated by constructing a nrdD-deficient mutant. In the murine abscess model, the parental W83 strain produced necrotic abscesses, while the nrdD-deficient mutant had almost lost this ability. Insertion of IS1598 into the nrdD-like gene promoter region may be related to the phenotypic differences in virulence among P. gingivalis strains through upregulation of the expression of this gene.

    Web of Science

    Scopus

    PubMed

    researchmap

  • Effects of new over-the-counter periodontal ointment-containing applicator with single-tuft brush on cytokine levels in gingival crevicular fluid during supportive periodontal therapy phase: a randomized double-blind clinical trial. 査読 国際誌

    K Takeuchi-Hatanaka, T Yasuda, Koji Naruishi, K Katsuragi-Fuke, J Inubushi, H Ootsuki, H Maeda, S Takashiba

    Journal of Periodontal Research   Vol.51 ( No.3 )   321 - 331   2016年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The levels of IL-1β, IL-6, IL-8 and TNF-α remained significantly lower in the test group compared to the placebo group. In the placebo group, when the probing pocket depth at baseline was 4 mm, IL-1β increased, particularly in the second molar tooth, and the greatest increase was seen when PPD at baseline was 5-6 mm. In the test group, IL-1β decreased markedly in cases with furcation involvement and low bleeding on probing at baseline. In both groups, IL-1β, IL-6 and TNF-α were closely correlated with each other.

    DOI: 10.1111/jre.12311

    PubMed

    researchmap

  • Production of germ cell-deficient salmonids by dead end gene knockdown, and their use as recipients for germ cell transplantation. 国際誌

    Goro Yoshizaki, Kuniko Takashiba, Shotaro Shimamori, Kiyoko Fujinuma, Shinya Shikina, Tomoyuki Okutsu, Sachi Kume, Makoto Hayashi

    Molecular reproduction and development   83 ( 4 )   298 - 311   2016年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We previously established a spermatogonial transplantation model in fish using triploid recipients. Although triploid salmonids are sterile, they carry a limited number of immature triploid germ cells that potentially compete with the donor-derived germ cells for their niche. We therefore assessed the biological characteristics of germ cell-deficient gonads in rainbow trout for their suitability as recipients for germ cell transplantation in this study. Antisense morpholino oligonucleotides against the dead end gene were microinjected into the fertilized eggs of rainbow trout to eliminate endogenous germ cells, leaving only their supporting cells. Unlike similar approaches performed in zebrafish and medaka, these germ cell-deficient rainbow trout did not show a male-biased sex ratio. Approximately 30,000 spermatogonia were then transplanted into the body cavities of both germ cell-deficient and control recipients. The donor-derived germ cells showed significantly higher proliferation in the gonads of germ cell-deficient recipients than those in the gonads of the control recipients. Finally, the applicability of the germ cell-deficient recipients for xenogeneic transplantation was evaluated by transplanting rainbow trout spermatogonia into germ cell-deficient masu salmon recipients. The resulting recipient salmon matured normally and produced trout gametes, and early survival of the resulting trout offspring was as high as that of the control offspring. Thus, dead end-knockdown salmonids appear to be ideal recipients for the intraperitoneal transplantation of spermatogonia.

    DOI: 10.1002/mrd.22625

    PubMed

    researchmap

  • Involvement of an Skp-Like Protein, PGN_0300, in the Type IX Secretion System of Porphyromonas gingivalis 査読 国際誌

    Yuko Taguchi, Keiko Sato, Hideharu Yukitake, Tetsuyoshi Inoue, Masaaki Nakayama, Mariko Naito, Yoshio Kondo, Konami Kano, Tomonori Hoshino, Koji Nakayama, Shogo Takashiba, Naoya Ohara

    INFECTION AND IMMUNITY   84 ( 1 )   230 - 240   2016年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    The oral Gram-negative anaerobic bacterium Porphyromonas gingivalis is an important pathogen involved in chronic periodontitis. Among its virulence factors, the major extracellular proteinases, Arg-gingipain and Lys-gingipain, are of interest given their abilities to degrade host proteins and process other virulence factors. Gingipains possess C-terminal domains (CTDs) and are translocated to the cell surface or into the extracellular milieu by the type IX secretion system (T9SS). Gingipains contribute to the colonial pigmentation of the bacterium on blood agar. In this study, Omp17, the PGN_0300 gene product, was found in the outer membrane fraction. A mutant lacking Omp17 did not show pigmentation on blood agar and showed reduced proteolytic activity of the gingipains. CTD-containing proteins were released from bacterial cells without cleavage of the CTDs in the omp17 mutant. Although synthesis of the anionic polysaccharide (A-LPS) was not affected in the omp17 mutant, the processing of and A-LPS modification of CTD-containing proteins was defective. PorU, a C-terminal signal peptidase that cleaves the CTDs of other CTD-containing proteins, was not detected in any membrane fraction of the omp17 mutant, suggesting that the defective maturation of CTD-containing proteins by impairment of Omp17 is partly due to loss of function of PorU. In the mouse subcutaneous infection experiment, the omp17 mutant was less virulent than the wild type. These results suggested that Omp17 is involved in P. gingivalis virulence.

    DOI: 10.1128/IAI.01308-15

    Web of Science

    PubMed

    researchmap

  • Cut-off values of Functional Independence Measure scores for discharge destination. 査読

    Koji Naruishi, Akiko Kunita, Toshihiko Nagata, Shogo Takashiba, Seiji Adachi

    Geriatrics & Gerontology International   Vol.15 ( No.5 )   670 - 671   2015年5月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/ggi.12430

    PubMed

    researchmap

  • With regard to our manuscripts on the commercial saliva substitute, Oralbalance®--its formula has been changed. 査読 国際誌

    Sugiura Y, Soga Y, Tanimoto I, Kokeguchi S, Morishige-Nishide S, Itami-Kono K, Takahashi K, Fujii N, Ishimaru F, Tanimoto M, Yamabe K, Tsutani S, Nishimura F, Takashiba S

    Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer   22 ( 12 )   3121 - 3122   2014年12月

     詳細を見る

  • Synthetic (+)-terrein suppresses interleukin-6/soluble interleukin-6 receptor induced-secretion of vascular endothelial growth factor in human gingival fibroblasts 査読 国際誌

    Hiroki Mandai, Kazuhiro Omori, Daisuke Yamamoto, Toki Tsumura, Kyouta Murota, Satoshi Yamamoto, Koichi Mitsudo, Soichiro Ibaragi, Akira Sasaki, Hiroshi Maeda, Shogo Takashiba, Seiji Suga

    BIOORGANIC & MEDICINAL CHEMISTRY   22 ( 19 )   5338 - 5344   2014年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Interleukin (IL)-6 is a proinflammatory cytokine that performs a wide variety of biological functions, including important roles in the progression of chronic inflammatory diseases such as periodontal disease. (+)-Terrein, a secondary bioactive fungal metabolite isolated from Aspergillus terreus, has various biological activities; however, its anti-inflammatory effects are still unknown. The purpose of this study was to examine the effect of synthetic (+)-terrein on IL-6 signaling and related protein production in human gingival fibroblasts. To our knowledge, this study is the first to report that synthetic (+)-terrein is not cytotoxic at concentrations less than 20 mu M and suppresses IL-6/soluble IL-6 receptor (sIL-6R)-induced phosphorylation of signal transducer and activator of transcription-3, extracellular signal-regulated kinase 1/2, and c-jun N-terminal kinase 1/2-signaling proteins that are downstream of IL-6 signaling. In addition, synthetic (+)-terrein suppresses IL-6/sIL-6R-induced vascular endothelial growth factor (VEGF) secretion in a concentration-dependent manner (p &lt; 0.01). These data suggest that synthetic (+)-terrein has potential anti-IL-6 signaling activity and suppresses VEGF-associated inflammatory disease progression. (C) 2014 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.bmc.2014.07.047

    Web of Science

    PubMed

    researchmap

  • Distribution of oral mucosal bacteria with mecA in patients undergoing hematopoietic cell transplantation 査読 国際誌

    Takayuki Ebinuma, Yoshihiko Soga, Takamaro Sato, Kazuyuki Matsunaga, Chieko Kudo, Hiroshi Maeda, Yoshinobu Maeda, Mitsune Tanimoto, Shogo Takashiba

    SUPPORTIVE CARE IN CANCER   22 ( 6 )   1679 - 1683   2014年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    We recently reported frequent detection of antibiotic-resistant bacteria on the oral mucosa during the period of hematopoietic cell transplantation (HCT) and suggested an association between oral mucositis and antibiotic-resistant bacterial infection. Methicillin-resistant Staphylococcus spp. were frequently detected, and the oral cavity may be a reservoir of the gene mediating methicillin resistance, mecA. Here, we examined the frequency of mecA carriers in patients undergoing HCT.
    Fifty-nine patients (male (M) = 37, female (F) = 22, 47.3 +/- 11.0 years) receiving HCT were enrolled in this study. Buccal swab samples were obtained four times from day -7 to day +20 (once/week), and mecA was detected by PCR. Fifty-two subjects without systemic disease, who completed dental treatment, especially periodontal treatment (M = 21, F = 31, 55.4 +/- 14.2 years), were also enrolled as controls and checked for mecA on the oral mucosa.
    Seventy-six percent (45/59) of the HCT patients carried mecA at least once in the study period (days -7 to +20), while no control subjects had mecA. The frequency of mecA carriers was 19.2 % from days -7 to -1, while it was significantly increased on days +7 to +13 and +14 to +20, with frequencies of 60.9 and 63.2 %, respectively (P &lt; 0.01, ANOVA).
    mecA was detected in oral mucosa of patients undergoing HCT. The high detection frequency of staphylococci resistant to penicillin and beta-lactams in our recent report was supported.

    DOI: 10.1007/s00520-014-2151-1

    Web of Science

    PubMed

    researchmap

  • Effect of Porphyromonas gingivalis outer membrane vesicles on gingipain-mediated detachment of cultured oral epithelial cells and immune responses 査読 国際誌

    Ryoma Nakao, Shogo Takashiba, Saori Kosono, Minoru Yoshida, Haruo Watanabe, Makoto Ohnishi, Hidenobu Senpuku

    MICROBES AND INFECTION   16 ( 1 )   6 - 16   2014年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Porphyromonas gingivalis is a major etiological agent of periodontal diseases and the outer membrane vesicles (OMVs) contain virulence factors such as LPS and gingipains. In this study, we investigated the potential role of the OMVs in host immune response and tissue destruction during P. gingivalis infection. Firstly, we found that sera from periodontitis patients had significantly stronger reactivity against an OMV-producing wild type strain than the isogenic OMV-depleted strain. OMVs were found to be highly antigenic, as absorption of patient sera with OMVs greatly reduced reactivity with whole cells of P. gingivalis. LC-MS/MS analysis of OMVs revealed multiple forms of gingipains and several gingipain-related proteins. Western blots of OMVs using patient sera revealed a conserved immunoreactive antigen profile resembling the profile of OMV antigens that were recognized by gingipain antiserum, suggesting a potential role of OMV-associated gingipains in triggering antibody-mediated immune responses to P. gingivalis infection. When OMVs were added to a monolayer of an oral squamous epithelial cell line, OMVs caused cell detachment, which was inhibited by preincubating OMVs with anti-gingipain antiserum. These data suggest that gingipain-laden OMVs may contribute to tissue destruction in periodontal diseases by serving as a vehicle for the antigens and active proteases. (C) 2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

    DOI: 10.1016/j.micinf.2013.10.005

    Web of Science

    PubMed

    researchmap

  • Predictors of improved functional outcome in elderly inpatients after rehabilitation: a retrospective study 査読 国際誌

    Koji Naruishi, Akiko Kunita, Katsuyuki Kubo, Toshihiko Nagata, Shogo Takashiba, Seiji Adachi

    CLINICAL INTERVENTIONS IN AGING   9   2133 - 2141   2014年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:DOVE MEDICAL PRESS LTD  

    Purpose: The number of elderly inpatients has been steadily increasing worldwide. However, the ability to predict the degree of improvement of functional capacity after comprehensive examination of elderly inpatients is still lacking. The purpose of this study was to investigate the predictors of improved functional outcome after rehabilitation of elderly inpatients.
    Methods: We performed a retrospective cohort study with 1,079 patients (age &lt;70 years: N=331, age &gt;= 70 years: N=748) who had been admitted to Tottori Municipal Hospital. Functional Independence Measure (FIM) scores were measured both at admission and discharge to calculate FIM gain and efficiency. Of these patients, 262 patients had oral examinations on admission. The Mann-Whitney U-test or chi-square test was used for statistical analyses. Conditional logistic regression analysis was used to compute the odds ratio (OR) and 95% confidence interval (CI). Cut-off values of FIM scores to determine if elderly inpatients were able to return home after discharge were determined using a receiver operating characteristic curve.
    Results: FIM scores, including FIM gain and efficiency, of elderly patients were significantly lower than those of middle-aged patients. Inability to close the lips and dysfunctional tongue movement, but not the loss of teeth, were correlated with a reduced improvement of FIM scores. Cognitive impairment and aspiration pneumonia, but not cerebrovascular disease, were also correlated with a reduced improvement of FIM scores. Interestingly, FIM scores were significantly lower in patients with both cerebrovascular disease and a loss of posterior occlusion. Factors shown to have a significant impact on the improvement of FIM scores included the stable posterior occlusion (OR: 2.23, 95% CI: 1.2-4.1), closed lips (OR: 5.15, 95% CI: 2.3-11.7), functional tongue movement (OR: 5.74, 95% CI: 3.0-11.0), presence of cognitive impairment (OR: 0.31, 95% CI: 0.17-0.49), and presence of aspiration pneumonia (OR: 0.27, 95% CI: 0.15-0.51).
    Conclusion: Age and disorder of oral function may be significant predictors of improved functional capacity after rehabilitation for elderly inpatients.

    DOI: 10.2147/CIA.S73388

    Web of Science

    PubMed

    researchmap

  • Cytokine expression in human dermal fibroblasts stimulated with eosinophil cationic protein measured by protein array 査読 国際誌

    Takamaro Sato, Yoshihiko Soga, Tomoko Yamaguchi, Michio Meguro, Hiroshi Maeda, Joji Tada, Takayuki Otani, Masaharu Seno, Shogo Takashiba

    ASIAN PACIFIC JOURNAL OF ALLERGY AND IMMUNOLOGY   31 ( 4 )   271 - 276   2013年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ALLERGY IMMUNOL SOC THAILAND,  

    Background: Eosinophil cationic protein (ECP) was reported previously to be involved in allergic inflammation with cytotoxic activity. On the other hand, recent studies showed that ECP did not induce cell death but inhibited the growth of cancer-derived cells. Our previous study indicated that human ECP enhanced differentiation of rat neonatal cardiomyocytes and stress fiber formation in Balb/c 3T3 mouse fibroblasts, while the effects of human ECP on human fibroblasts are unknown.
    Objective: The present study was performed to determine the effects of human ECP on cytokine expression in human fibroblasts by protein array.
    Methods: The effects of recombinant human ECP (rhECP) on normal human dermal fibroblasts (NHDF) were examined by assaying cell growth. Furthermore, cytokine expression of NHDF stimulated by ECP, which could influence cell growth, was evaluated by protein array.
    Results: ECP was not cytotoxic but enhanced the growth of NHDF. The peak rhECP concentration that enhanced the cell counts by 1.56-fold was 100 ng/mL, which was significantly different from cultures without ECP stimulation (ANOVA/ Scheffe's test, P &lt; 0.05). Array analyses indicated that ciliary neurotrophic factor (CNTF), neutrophil-activating peptide (NAP)-2, and neurotrophin (NT)-3 were significantly upregulated in NHDF stimulated with 100 ng/mL ECP compared to those without stimulation.
    Conclusion: ECP is not cytotoxic but enhances the growth of NHDF. CNTF, NAP-2, and NT-3 were suggested to be involved in enhancing the growth of NHDF. These findings will contribute to determination of the role of ECP in allergic inflammation.

    DOI: 10.12932/AP0287.31.4.2013

    Web of Science

    PubMed

    researchmap

  • Serum antibody response to group II chaperonin from Methanobrevibacter oralis and human chaperonin CCT 査読 国際誌

    Kimito Hirai, Hiroshi Maeda, Kazuhiro Omori, Tadashi Yamamoto, Susumu Kokeguchi, Shogo Takashiba

    PATHOGENS AND DISEASE   68 ( 1 )   12 - 19   2013年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Both group I (HSP60) and group II (CCT) chaperonins are targets of autoantibodies. Autoimmune reactions to HSP60 have been well characterized, while immune reactions to group II chaperonin have not been clarified. Methanobrevibacter oralis is a suspected periodontal pathogen with group II chaperonin. In this study, serum responses to M.oralis chaperonin, human HSP60, and CCT subunits were examined using sera from patients with periodontitis and autoimmune diseases. In comparison with healthy controls, periodontitis patients showed significantly higher responses to CCT4 and CCT8 on dot blot analysis. Signals for CCT3 and CCT8 in autoimmune disease patients were significantly higher than in controls. Significant differences were also demonstrated by Western blotting in anti-CCT4 response in both patient groups. All subjects showed strong reactivity to M.oralis chaperonin and faint signals to human HSP60. Autoantibodies were raised against CCT rather than HSP60; and CCT3, CCT4, and CCT8 were shown to be the main targets. Host immune systems may be frequently exposed to chaperonins of Archaea in various habitats. Although further studies of the cross-reactivity between M.oralis chaperonin and human CCT are required, anti-CCT autoantibodies may be involved in the pathogenesis of periodontitis and autoimmune diseases.

    DOI: 10.1111/2049-632X.12041

    Web of Science

    PubMed

    researchmap

  • Antibiotic sensitivity of bacteria on the oral mucosa after hematopoietic cell transplantation 査読 国際誌

    Yoshihiko Soga, Yoshinobu Maeda, Mitsune Tanimoto, Takayuki Ebinuma, Hiroshi Maeda, Shogo Takashiba

    SUPPORTIVE CARE IN CANCER   21 ( 2 )   367 - 368   2013年2月

     詳細を見る

    記述言語:英語   出版者・発行元:SPRINGER  

    DOI: 10.1007/s00520-012-1602-9

    Web of Science

    PubMed

    researchmap

  • Assessment of the Plasma/Serum IgG Test to Screen for Periodontitis 査読 国際誌

    C. Kudo, K. Naruishi, H. Maeda, Y. Abiko, T. Hino, M. Iwata, C. Mitsuhashi, S. Murakami, T. Nagasawa, T. Nagata, S. Yoneda, Y. Nomura, T. Noguchi, Y. Numabe, Y. Ogata, T. Sato, H. Shimauchi, K. Yamazaki, A. Yoshimura, S. Takashiba

    JOURNAL OF DENTAL RESEARCH   91 ( 12 )   1190 - 1195   2012年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE PUBLICATIONS INC  

    Chronic periodontitis is a silent infectious disease prevalent worldwide and affects lifestyle-related diseases. Therefore, efficient screening of patients is essential for general health. This study was performed to evaluate prospectively the diagnostic utility of a blood IgG antibody titer test against periodontal pathogens. Oral examination was performed, and IgG titers against periodontal pathogens were measured by ELISA in 1,387 individuals. The cut-off value of the IgG titer was determined in receiver operating characteristic curve analysis, and changes in periodontal clinical parameters and IgG titers by periodontal treatment were evaluated. The relationships between IgG titers and severity of periodontitis were analyzed. The best cut-off value of IgG titer against Porphyromonas gingivalis for screening periodontitis was 1.682. Both clinical parameters and IgG titers decreased significantly under periodontal treatment. IgG titers of periodontitis patients were significantly higher than those of healthy controls, especially in those with sites of probing pocket depth over 4 mm. Multiplied cut-off values were useful to select patients with severe periodontitis. A blood IgG antibody titer test for Porphyromonas gingivalis is useful to screen hitherto chronic periodontitis patients (ClinicalTrials.gov number NCT01658475).

    DOI: 10.1177/0022034512461796

    Web of Science

    PubMed

    researchmap

  • Assessment of the plasma/serum IgG test to screen for periodontitis. 査読

    C Kudo, Koji Naruishi, H Maeda, Y Abiko, T Hino, M Iwata, C Mitsuhashi, S Murakami, T Nagasawa, Toshihiko Nagata, Satoshi Yoneda, Y Nomura, T Noguchi, Y Numabe, Y Ogata, T Sato, H Shimauchi, K Yamazaki, A Yoshimura, S Takashiba

    Journal of Dental Research   Vol.91 ( No.12 )   1190 - 1195   2012年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Chronic periodontitis is a silent infectious disease prevalent worldwide and affects lifestyle-related diseases. Therefore, efficient screening of patients is essential for general health. This study was performed to evaluate prospectively the diagnostic utility of a blood IgG antibody titer test against periodontal pathogens. Oral examination was performed, and IgG titers against periodontal pathogens were measured by ELISA in 1,387 individuals. The cut-off value of the IgG titer was determined in receiver operating characteristic curve analysis, and changes in periodontal clinical parameters and IgG titers by periodontal treatment were evaluated. The relationships between IgG titers and severity of periodontitis were analyzed. The best cut-off value of IgG titer against Porphyromonas gingivalis for screening periodontitis was 1.682. Both clinical parameters and IgG titers decreased significantly under periodontal treatment. IgG titers of periodontitis patients were significantly higher than those of healthy controls, especially in those with sites of probing pocket depth over 4 mm. Multiplied cut-off values were useful to select patients with severe periodontitis. A blood IgG antibody titer test for Porphyromonas gingivalis is useful to screen hitherto chronic periodontitis patients.

    DOI: 10.1177/0022034512461796

    PubMed

    researchmap

  • Smad2 Decelerates Re-epithelialization during Gingival Wound Healing 査読 国際誌

    K. Tomikawa, T. Yamamoto, N. Shiomi, M. Shimoe, S. Hongo, K. Yamashiro, T. Yamaguchi, H. Maeda, S. Takashiba

    JOURNAL OF DENTAL RESEARCH   91 ( 8 )   764 - 770   2012年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE PUBLICATIONS INC  

    During periodontal regeneration, inhibition of gingival downgrowth is necessary to promote migration of mesenchymal cells into the defects. Transforming growth factor (TGF)-beta is a pleiotropic cytokine that has numerous cell functions, including regulation of epithelial growth. Recent studies have shown that Smad2, a downstream transcription factor of TGF-beta, plays crucial roles in wound healing in the epithelia. Therefore, we investigated the effects of Smad2 overexpression on re-epithelialization of gingival wounds. Transgenic mice overexpressing smad2 driven by the keratin 14 promoter (k14-smad2) were confirmed to have significant Smad2 phosphorylation in gingival basal epithelia. Punch wounds were made in the palatal gingiva, and wound healing was assessed histologically for 7 days. Re-epithelialization was significantly retarded on day 2, while collagen deposition was enhanced on day 7 in k14-smad2 compared with wild-type mice. Moreover, expression of keratin 16 (K16), an indicator of keratinocyte migration, was significantly inhibited in wound-edge keratinocytes in k14-smad2. The inhibition of K16 coincided with the induction of Smad2 in the corresponding epithelia, while BrdU incorporation was unaffected. These results indicated that Smad2 has inhibitory effects in regulating keratinocyte migration during gingival wound healing. TGF-beta/Smad2 signaling mediating alteration of K16 expression must be tightly regulated during periodontal regeneration.

    DOI: 10.1177/0022034512451449

    Web of Science

    PubMed

    researchmap

  • Histological and immunohistochemical features of gingival enlargement in a patient with AML 査読

    Norihiro Sonoi, Yoshihiko Soga, Hiroshi Maeda, Koichi Ichimura, Tadashi Yoshino, Kazutoshi Aoyama, Nobuharu Fujii, Yoshinobu Maeda, Mitsune Tanimoto, Richard Logan, Judith Raber-Durlacher, Shogo Takashiba

    ODONTOLOGY   100 ( 2 )   254 - 257   2012年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Here, we discuss the pathophysiology of leukemia-associated gingival enlargement based on a case of acute myelomonocytic leukemia (AML-M4) with typical gingival enlargement. Uniquely, this patient was well enough to allow full periodontal examination and incisional gingival biopsy to be performed both before and after chemotherapy. The patient was a 39-year-old Japanese woman with AML-M4 showing gingival enlargement. Histological and immunohistochemical features of gingiva and bacterial counts in the periodontal pockets were examined before and after chemotherapy. The results were as follows: (1) infiltration of myelomonocytic blasts in enlarged gingiva; (2) resolution of gingival enlargement with complete remission of AML by anticancer chemotherapy; and (3) the numbers of bacteria in the periodontal pockets were not high and were not altered before or after chemotherapy. In patients with AML-M4, remarkable mucosal enlargement is not generally observed in the body except in the gingiva. We hypothesized that antigens derived from periodontal bacteria, even if they are not present in large numbers, could act as chemoattractants for myelomonocytic leukemic cells.

    DOI: 10.1007/s10266-011-0051-0

    Web of Science

    PubMed

    researchmap

  • Relationship between Periodontitis-Related Antibody and Frequent Exacerbations in Chronic Obstructive Pulmonary Disease 査読 国際誌

    Tamaki Takahashi, Shigeo Muro, Naoya Tanabe, Kunihiko Terada, Hirofumi Kiyokawa, Susumu Sato, Yuma Hoshino, Emiko Ogawa, Kazuko Uno, Koji Naruishi, Shogo Takashiba, Michiaki Mishima

    PLOS ONE   7 ( 7 )   e40570   2012年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Background: To identify patients with chronic obstructive pulmonary disease (COPD) who are susceptible to frequent exacerbations is important. Although periodontitis aggravated by poor oral hygiene might increase the risk of lower respiratory tract infection, the relationship between periodontitis and COPD exacerbations remains unknown. This prospective cohort study investigates the relationship between periodontitis-related antibody and exacerbation frequency over a one-year period.
    Methods: We assessed an IgG antibody titer against Porphyromonas gingivalis, which is a major pathogen of periodontitis, and then prospectively followed up 93 individuals over one year to detect exacerbations.
    Results: The numbers of exacerbations and the rate of individuals with frequent exacerbations (at least two per year) were significantly lower in patients with higher IgG titer than those with normal IgG titer (0.8 vs. 1.2 per year, p = 0.045 and 14.3 vs. 38.6%, p = 0.009, respectively). Multivariate logistic regression analysis showed that being normal-IgG titer for periodontitis-related antibody significantly increased the risk of frequent exacerbations (relative risk, 5.27, 95% confidence interval, 1.30-25.7; p = 0.019) after adjusting for other possible confounders, such as a history of exacerbations in the past year, disease severity, COPD medication and smoking status.
    Conclusions: Normal-IgG titer for periodontitis-related antibody can be an independent predictor of frequent exacerbations. Measuring periodontitis-related antibody titers might be useful to identify patients with susceptibility to frequent exacerbations so that an aggressive prevention strategy can be designed.

    DOI: 10.1371/journal.pone.0040570

    Web of Science

    PubMed

    researchmap

  • Influence of resin coating materials on Porphyromonas gingivalis attachment 査読

    Ai Kumada, Yoshizo Matsuka, Atsushi Mine, Mitsuaki Ono, Junji Uehara, Norihiro Sonoi, Takashi Ito, Shogo Takashiba, Takuo Kuboki

    DENTAL MATERIALS JOURNAL   31 ( 1 )   86 - 91   2012年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPANESE SOC DENTAL MATERIALS DEVICES  

    Resin coating materials have been used for composite resin or provisional restoration in order to prevent plaque accumulation on their surfaces. However, it is not clear whether the coating materials influence attachment of periodontal bacteria. Therefore, we investigated the effect of resin coating materials on the attachment of Porphyromonas gingivalis (Pg). The polymerized auto cure resin plates were coated with two resin coating materials. To estimate the Pg attachment, each plate was immersed in brain heart infusion medium containing Pg. The quantity of bacteria attached on each plate was evaluated by crystal violet quantification. Morphological change of Pg was recorded using scanning electron microscopy. Both coating groups presented significantly lower Pg attachment compared to the control. The Pg shapes on the plates with resin coating materials were similar to the non-treated control plates. The resin coating materials clearly prevent Pg attachment on the polymerized auto cure resin plate.

    DOI: 10.4012/dmj.2011-164

    Web of Science

    CiNii Article

    researchmap

  • Outer Membrane Vesicles of Porphyromonas gingivalis Elicit a Mucosal Immune Response 査読 国際誌

    Ryoma Nakao, Hideki Hasegawa, Kuniyasu Ochiai, Shogo Takashiba, Akira Ainai, Makoto Ohnishi, Haruo Watanabe, Hidenobu Senpuku

    PLOS ONE   6 ( 10 )   e26163   2011年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    We previously reported that mutation of galE in Porphyromonas gingivalis has pleiotropic effects, including a truncated lipopolysaccharide (LPS) O-antigen and deglycosylation of the outer membrane protein OMP85 homolog. In the present study, further analysis of the galE mutant revealed that it produced little or no outer membrane vesicles (OMVs). Using three mouse antisera raised against whole cells of the P. gingivalis wild type strain, we performed ELISAs to examine the reactivity of these antisera with whole cells of the wild type or the galE mutant. All three antisera had significantly lower reactivity against the galE mutant compared to wild type. OMVs, but not LPS, retained the immunodominant determinant of P. gingivalis, as determined by ELISAs (with wild type LPS or OMVs as antigen) and absorption assays. In addition, we assessed the capacity of OMVs as a vaccine antigen by intranasal immunization to BALB/c mice. Synthetic double-stranded RNA polyriboinosinic polyribocytidylic acid [Poly (I: C)], an agonist of Toll-like receptor 3 (TLR3), was used as the mucosal adjuvant. Vaccination with OMV elicited dramatically high levels of P. gingivalis-specific IgA in nasal washes and saliva, as well as serum IgG and IgA. In conclusion, the OMVs of P. gingivalis have an important role in mucosal immunogenicity as well as in antigenicity. We propose that P. gingivalis OMV is an intriguing immunogen for development of a periodontal disease vaccine.

    DOI: 10.1371/journal.pone.0026163

    Web of Science

    PubMed

    researchmap

  • Bacterial substitution of coagulase-negative staphylococci for streptococci on the oral mucosa after hematopoietic cell transplantation 査読 国際誌

    Yoshihiko Soga, Yoshinobu Maeda, Fumihiko Ishimaru, Mitsune Tanimoto, Hiroshi Maeda, Fusanori Nishimura, Shogo Takashiba

    SUPPORTIVE CARE IN CANCER   19 ( 7 )   995 - 1000   2011年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Coagulase-negative staphylococci (CoNS) are frequently isolated from blood cultures of hematopoietic cell transplantation (HCT) patients. Generally, the use of central venous catheters is recognized as a significant risk factor for CoNS infection, while the impact of CoNS infection from oral ulcerative mucositis, which occurs frequently in HCT, may be underestimated. Here, we examined the bacteria on the buccal mucosa after HCT.
    Sixty-one patients were examined for bacteria on the buccal mucosa routinely once a week from 1 week before to 3 weeks after allogeneic HCT. Subjects were divided into groups with short and long periods of antibiotic use, and differences in bacterial substitution were evaluated. The relationships between type of HCT (conventional HCT or RIST) and bacterial substitution were also evaluated.
    The changes in detection frequencies of CoNS and alpha-streptococci from before to 3 weeks after HCT were significant (P &lt; 0.05, chi (2) test): 14.5-53.3% and 92.7-53.1%, respectively. Significant bacterial substitution of CoNS for streptococci was observed in the long-term antibiotic use group (P &lt; 0.05, chi (2) test), but also occurred in cases with short-term or no antibiotic use. No relationships between type of HCT (conventional HCT or RIST) were observed.
    Bacterial substitution of CoNS for streptococci occurred frequently on the buccal mucosa after HCT. In addition to antibiotic use, environmental factors may be involved in bacterial substitution. It is important to consider the presence of oral mucositis in CoNS infection after HCT.

    DOI: 10.1007/s00520-010-0923-9

    Web of Science

    PubMed

    researchmap

  • Chronic periodontitis with multiple risk factor syndrome: a case report. 査読 国際誌

    Shimoe M, Yamamoto T, Iwamoto Y, Shiomi N, Maeda H, Nishimura F, Takashiba S

    Journal of the International Academy of Periodontology   13 ( 2 )   40 - 47   2011年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:2  

    PubMed

    researchmap

  • Specific In Situ Visualization of Plasma Cells Producing Antibodies against Porphyromonas gingivalis in Gingival Radicular Cyst: Application of the Enzyme-Labeled Antigen Method 査読 国際誌

    Shinya Tsuge, Yasuyoshi Mizutani, Kazuhiro Matsuoka, Tatsuya Sawasaki, Yaeta Endo, Koji Naruishi, Hiroshi Maeda, Shogo Takashiba, Kazuya Shiogama, Ken-ichi Inada, Yutaka Tsutsumi

    JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY   59 ( 7 )   673 - 689   2011年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE PUBLICATIONS LTD  

    The enzyme-labeled antigen method was applied to visualize plasma cells producing antibodies to Porphyromonas gingivalis, flora of the human oral cavity. Antibodies to P. gingivalis have reportedly been detected in sera of patients with periodontitis. Biotinylated bacterial antigens, Ag53, and four gingipain domains (Arg-pro, Arg-hgp, Lys-pro, and Lys-hgp) were prepared by the cell-free protein synthesis system using the wheat germ extract. In paraformaldehyde-fixed frozen sections of rat lymph nodes experimentally immunized with Ag53-positive and Ag53-negative P. gingivalis, plasma cells were labeled with biotinylated Arg-hgp and Lys-hgp. Antibodies to Ag53 were detected only in the nodes immunized with Ag53-positive bacteria. In two of eight lesions of gingival radicular cyst with inflammatory infiltration, CD138-positive plasma cells in frozen sections were signalized for Arg-hgp and Lys-hgp. An absorption study using unlabeled antigens confirmed the specificity of staining. The AlphaScreen method identified the same-type antibodies in tissue extracts but not in sera. Antibodies to Ag53, Arg-pro, and Lys-pro were undetectable. In two cases, serum antibodies to Arg-hgp and Lys-hgp were AlphaScreen positive, whereas plasma cells were scarcely observed within the lesions. These findings indicate the validity of the enzyme-labeled antigen method. This is the very first application of this novel histochemical technique to human clinical samples. (J Histochem Cytochem 59:673-689, 2011)

    DOI: 10.1369/0022155411408906

    Web of Science

    PubMed

    researchmap

  • Discovery of a patient with strongly suspected bullous pemphigoid in a ward by oral health care providers 査読 国際誌

    N. Kanda, Y. Soga, M. Meguro, A. Tanabe, Y. Yagi, Y. Himuro, Y. Fujiwara, S. Takashiba, N. Kobayashi

    INTERNATIONAL JOURNAL OF DENTAL HYGIENE   9 ( 2 )   159 - 162   2011年5月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Objectives: Oral health care providers may discover systemic diseases incidentally from signs observed in the oral cavity. Here, we report a case in which oral health care providers in a hospital discovered a patient with strongly suspected bullous pemphigoid (BP), which is a relatively rare but important disease, in a ward. Methods: The patient was a 78-year-old Japanese woman admitted to our hospital because of severe Alzheimer&apos;s disease. We discovered recurrent ulcers in the oral mucosa and skin when performing oral care in her ward. Biopsy could not be performed safely because of involuntary biting. We performed blood tests for anti-BP180-NC16a antibody, which is autoantibody specific for BP. Results: The patient had a very high anti-BP180-NC16a antibody titre. We consulted a dermatologist regarding her clinical course and the clinical features of the oral mucosa and skin along with blood test results. BP was very strongly suspected. Discussion: In cases in which oral health care providers suspect their patients may have BP, appropriate examination and provision of information to the doctor are important. Oral health care providers should have knowledge about systemic diseases, the signs of which appear in oral cavity to avoid missing important systemic diseases.

    DOI: 10.1111/j.1601-5037.2010.00448.x

    Web of Science

    PubMed

    researchmap

  • IMMUNE RESPONSES TO PORPHYROMONAS GINGIVALIS INFECTION SUPPRESS SYSTEMIC INFLAMMATORY RESPONSE IN EXPERIMENTAL MURINE MODEL 査読

    K. Naruishi, K. Omori, H. Maeda, N. Sonoi, K. Funakoshi, K. Hirai, M. Ishii, K. Kubo, H. Kobayashi, T. Tomiyama, D. Yamamoto, I. Tanimoto, K. Kunimatsu, S. Takashiba

    JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS   25 ( 2 )   195 - 202   2011年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BIOLIFE SAS  

    Periodontitis is localized infectious disease caused by periodontopathic bacteria such as Porphyromonas gingivalis (T: gingivalis), and the severity correlates to significance of immune responses. Recently, it has been reported that periodontitis is associated with the development of systemic disease such as diabetes and atherosclerosis because of increasing invasion of oral pathogens to the circulation. However, the association between local and systemic infectious responses is still unclear. In the present study, we examined the differences of biological responses in animals with or without bacterial infection. After Balb/c mice were infected subcutaneously with live P gingivalis W83, serum, skin and liver were collected according to experimental protocol. The skin and liver tissues were observed pathologically by haematoxylin-eosin staining, and serum IL-6 levels were measured using ELISA method. Throughout the experimental period, conditions of the mice were observed continuously. As expected, severe infiltration of leukocytes were observed at inflamed skin corresponding to the number of bacterial challenges. Although no inflammatory appearance of skin was observed, serum IL-6 levels were increased dramatically (P &lt; 0.01, Student&apos;s t-test) and liver tissues were injured in the mice without bacterial challenge. Interestingly, although severe inflammatory appearance of the skin was observed, serum IL-6 levels were not increased and no inflammatory responses were observed in the liver of the 3-times bacterially challenged group. Importantly, immunoglobulin G against P gingivalis W83 was detected in the blood of mice with 3-times bacterial challenge corresponding to improvement of weight loss and survival. In conclusion, although multiple infections develop severe localized inflammation, the immune system should be sufficient to protect the systemic inflammatory responses.

    Web of Science

    Scopus

    PubMed

    researchmap

  • Progress of oral care and reduction of oral mucositis-a pilot study in a hematopoietic stem cell transplantation ward 査読 国際誌

    Yoshihiko Soga, Yuko Sugiura, Kanayo Takahashi, Hitomi Nishimoto, Yoshinobu Maeda, Mitsune Tanimoto, Shogo Takashiba

    SUPPORTIVE CARE IN CANCER   19 ( 2 )   303 - 307   2011年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Oral mucositis is a common symptomatic complication associated with hematopoietic stem cell transplantation (HCT). We use simple strategies aimed to reduce oral mucositis by keeping the oral cavity clean and moist. Here, we report on the progress of oral care and the changes in the degree of oral mucositis. The purpose of this pilot study is to evaluate the effects of our strategies on the prevalence and the severity of oral mucositis.
    Fifty-three consecutive patients from 2003 to 2006 administered with conventional allogeneic HCT were enrolled in this study. The degree of oral mucositis was evaluated daily in all patients. Our oral care program was divided into two periods: "examination and trial period (2003 and 2004)" and "intensive oral care period (2005 and 2006)." In the latter, an oral care regimen was carried out systematically by a multidisciplinary team.
    Using our oral care strategies, the prevalence of ulcerative oral mucositis was decreased significantly. The rate was reduced from 76% (10 of 13) of patients with ulcerative oral mucositis in 2003 to only 20% (3 of 15) in 2006.
    Our pilot study suggests that oral mucositis in HCT patients can be alleviated by simple strategies aimed at keeping the oral cavity clean and moist.

    DOI: 10.1007/s00520-010-1002-y

    Web of Science

    PubMed

    researchmap

  • FGF-2 Stimulates Periodontal Regeneration: Results of a Multi-center Randomized Clinical Trial 査読 国際誌

    M. Kitamura, M. Akamatsu, M. Machigashira, Y. Hara, R. Sakagami, T. Hirofuji, T. Hamachi, K. Maeda, M. Yokota, J. Kido, T. Nagata, H. Kurihara, S. Takashiba, T. Sibutani, M. Fukuda, T. Noguchi, K. Yamazaki, H. Yoshie, K. Ioroi, T. Arai, T. Nakagawa, K. Ito, S. Oda, Y. Izumi, Y. Ogata, S. Yamada, H. Shimauchi, K. Kunimatsu, M. Kawanami, T. Fujii, Y. Furuichi, T. Furuuchi, T. Sasano, E. Imai, M. Omae, S. Yamada, M. Watanuki, S. Murakami

    JOURNAL OF DENTAL RESEARCH   90 ( 1 )   35 - 40   2011年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE PUBLICATIONS INC  

    The efficacy of the local application of recombinant human fibroblast growth factor-2 (FGF-2) in periodontal regeneration has been investigated. In this study, a randomized, double-blind, placebo-controlled clinical trial was conducted in 253 adult patients with periodontitis. Modified Widman periodontal surgery was performed, during which 200 mu L of the investigational formulation containing 0% (vehicle alone), 0.2%, 0.3%, or 0.4% FGF-2 was administered to 2- or 3-walled vertical bone defects. Each dose of FGF-2 showed significant superiority over vehicle alone (p &lt; 0.01) for the percentage of bone fill at 36 wks after administration, and the percentage peaked in the 0.3% FGF-2 group. No significant differences among groups were observed in clinical attachment regained, scoring approximately 2 mm. No clinical safety problems, including an abnormal increase in alveolar bone or ankylosis, were identified. These results strongly suggest that topical application of FGF-2 can be efficacious in the regeneration of human periodontal tissue that has been destroyed by periodontitis.

    DOI: 10.1177/0022034510384616

    Web of Science

    PubMed

    researchmap

  • Prognosis of Periodontitis Recurrence After Intensive Periodontal Treatment Using Examination of Serum IgG Antibody Titer Against Periodontal Bacteria 査読 国際誌

    Noriko Sugi, Koji Naruishi, Chieko Kudo, Aya Hisaeda-Kako, Takayuki Kono, Hiroshi Maeda, Shogo Takashiba

    JOURNAL OF CLINICAL LABORATORY ANALYSIS   25 ( 1 )   25 - 32   2011年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Chronic periodontitis is associated with systemic diseases such as atherosclerosis. In this study, we evaluated the efficacy of serum IgG antibody titer to periodontal bacteria for prognosis of periodontitis recurrence during supportive periodontal therapy (SPT) phase. The 139 patients during SPT phase were selected and divided to two groups as follows: "Stable" and "Recurrence" group at SPT phase for case control study: "High IgG titer" and "Normal IgG titer" group before transition to SPT phase for cohort study. We examined whether clinical findings or serum IgG antibody titers to periodontal bacteria are risk factors for the development of periodontitis recurrence. Case control study showed that there were significant differences between the stable and recurrence groups in age and number of teeth. The serum IgG antibody titer to Eikenella corrodens FDC1073, Porphyromonas gingivalis SU63, and Campylobacter rectus ATCC33238 was significantly higher in the recurrence group. Next, we found, that the recurrence ratio in the high IgG titer group to Gram-negative obligate anaerobe, Prevotella intermedia, Treponema denticola, and C. rectus was significantly higher than that of the normal IgG titer group. Taken together, serum IgG antibody titer test is useful in the prognosis of periodontitis recurrence during the SPT phase. J. Clin. Lab. Anal. 25:25-32, 2011. (C) 2011 Wiley-Liss, Inc.

    DOI: 10.1002/jcla.20381

    Web of Science

    PubMed

    researchmap

  • Clinical and Immunological Assessment of Periodontal Disease in Japanese Leprosy Patients 査読

    Hideki Ohyama, Hiroshi Hongyo, Naoko Shimizu, Yoshikazu Shimizu, Fusanori Nishimura, Masatsugu Nakagawa, Hideo Arai, Nahoko Kato-Kogoe, Nobuyuki Terada, Atsushi Nagai, Shogo Takashiba, Hidemi Kurihara, Yoshio Nomura, Yoji Murayama

    JAPANESE JOURNAL OF INFECTIOUS DISEASES   63 ( 6 )   427 - 432   2010年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATL INST INFECTIOUS DISEASES  

    Periodontitis is a chronic inflammatory disease caused by the infection of periodontopathic bacteria in dental plaque However, an individual's susceptibility to this disease appears to be associated with multiple genetic factors, as seen in the case of leprosy In order to gain a better understanding of the pathophysiology of periodontal disease in subjects with leprosy, we investigated the clinical features of periodontitis and the immunological responses against periodontopathic bacteria in 382 subjects with a history of leprosy and 451 age-matched control subjects The prevalence of periodontitis and the degree of periodontal pocket depth were found to be significantly higher in leprosy patients than in age-matched controls Furthermore, a comparison of the clinical parameters of lepromatous leprosy (L-lep) and tuberculoid leprosy (T-lep) patients showed that the probing pocket depth of L-lep patients with periodontal disease was significantly higher than that for T-lep patients In contrast, serum IgG titers against Porphyromonas gingivalis in L-lep patients were significantly lower than in T-lep patients These results imply that L-lep patients show more severe periodontal disease than T-lep patients or age-matched control subjects, and that low humoral immunity against P gingivalis might be one of the genetic factors determining periodontal disease susceptibility in leprosy patients

    Web of Science

    PubMed

    researchmap

  • Rapid detection of mecA and spa by the loop-mediated isothermal amplification (LAMP) method 査読 国際誌

    Y. Koide, H. Maeda, K. Yamabe, K. Naruishi, T. Yamamoto, S. Kokeguchi, S. Takashiba

    LETTERS IN APPLIED MICROBIOLOGY   50 ( 4 )   386 - 392   2010年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    Aim:
    To develop a detection assay for staphylococcal mecA and spa by using loop-mediated isothermal amplification (LAMP) method.
    Methods and Results:
    Staphylococcus aureus and other related species were subjected to the detection of mecA and spa by both PCR and LAMP methods. The LAMP successfully amplified the genes under isothermal conditions at 64 degrees C within 60 min, and demonstrated identical results with the conventional PCR methods. The detection limits of the LAMP for mecA and spa, by gel electrophoresis, were 102 and 10 cells per tube, respectively. The naked-eye inspections were possible with 103 and 10 cells for detection of mecA and spa, respectively. The LAMP method was then applied to sputum and dental plaque samples. The LAMP and PCR demonstrated identical results for the plaque samples, although frequency in detection of mecA and spa by the LAMP was relatively lower for the sputum samples when compared to the PCR methods.
    Conclusion:
    Application of the LAMP enabled a rapid detection assay for mecA and spa. The assay may be applicable to clinical plaque samples.
    Significance and Impact of the Study:
    The LAMP offers an alternative detection assay for mecA and spa with a great advantage of the rapidity.

    DOI: 10.1111/j.1472-765X.2010.02806.x

    Web of Science

    PubMed

    CiNii Article

    researchmap

  • Antigenic group II chaperonin in Methanobrevibacter oralis may cross-react with human chaperonin CCT 査読 国際誌

    K. Yamabe, H. Maeda, S. Kokeguchi, Y. Soga, M. Meguro, K. Naruishi, S. Asakawa, S. Takashiba

    MOLECULAR ORAL MICROBIOLOGY   25 ( 2 )   112 - 122   2010年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    P&gt;Methanobrevibacter oralis is an archaeal species frequently isolated from sites of severe periodontitis. However, its pathogenic roles remain unclear. Here, we aimed to isolate group II chaperonin from M. oralis and examine its antigenicity. The genes encoding two chaperonin subunits (Cpn-1 and Cpn-2) were cloned from M. oralis using polymerase chain reaction and genome walking procedures. Recombinant proteins Cpn-1 and Cpn-2 were generated, and the reactivities of sera from patients with periodontitis were examined by Western immunoblotting. The open reading frames of Cpn-1 and Cpn-2 genes consisted of 1641 and 1614 base pairs, respectively. Putative ATP-binding domains conserved among the chaperonin family were observed in both genes. The deduced amino acid sequences of the two genes showed 28.8-40.0% identity to each of the subunits of human CCT (CCT1-8). Thirty and 29 of 36 patients&apos; sera reacted with the recombinant Cpn-1 and recombinant Cpn-2, respectively. Western immunoblotting using antiserum against human CCT subunits indicated that anti-CCT3 and anti-CCT8 antibodies recognized recombinant Cpn-1. In addition, anti-CCT1, CCT3, CCT6, and CCT8 antibodies recognized an antigen of approximately 60 kDa in M. oralis. The results suggested that the chaperonin subunits of M. oralis were antigenic molecules that were recognized by periodontitis patients and that may cross-react with human chaperonin CCT.

    DOI: 10.1111/j.2041-1014.2009.00548.x

    Web of Science

    PubMed

    researchmap

  • Highly expressed genes in a rough-colony-forming phenotype of Aggregatibacter actinomycetemcomitans: implication of a mip-like gene for the invasion of host tissue 査読 国際誌

    Takemasa Maeda, Hiroshi Maeda, Kokoro Yamabe, Junji Mineshiba, Ichiro Tanimoto, Tadashi Yamamoto, Koji Naruishi, Susumu Kokeguchi, Shogo Takashiba

    FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY   58 ( 2 )   226 - 236   2010年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Aggregatibacter actinomycetemcomitans, a potent pathogen of periodontitis, typically grows as a rough and adherent colony on primary isolated cultures. The colony transforms into a smooth phenotype during repeated subculture. In this study, we aimed to identify highly expressed genes in the rough-colony-forming phenotype for isolation of host-induced genes. Using a cDNA-subtractive hybridization technique, three genes, homologous to a macrophage infectivity potentiator gene (mip), peroxiredoxin gene (prx) and outer membrane protein gene (ompA), were identified. The expression levels of these genes in the rough-colony-forming phenotype were 4-10-fold higher as compared with the smooth-colony-forming phenotype. Attention was focused on the mip-like gene, and a recombinant protein and a deficient mutant were constructed. The recombinant protein reacted with sera from patients with periodontitis, suggesting the production of the Mip-like protein in periodontal lesions. Viable quantitative invasion assay demonstrated that the viable cell counts of the wild-type strain that invaded HeLa cells were more than fourfold as compared with the mip-deficient mutant. The expression of the mip-like gene, prx-like gene and ompA-like gene may be enhanced in the host, and the mip-like gene may play an important role in the infection of A. actinomycetemcomitans, especially in its invasion of the epithelium.

    DOI: 10.1111/j.1574-695X.2009.00624.x

    Web of Science

    PubMed

    researchmap

  • Total bacterial counts on oral mucosa after using a commercial saliva substitute in patients undergoing hematopoietic cell transplantation 査読 国際誌

    Yuko Sugiura, Yoshihiko Soga, Kokoro Yamabe, Soichiro Tsutani, Ichiro Tanimoto, Hiroshi Maeda, Susumu Kokeguchi, Nobuharu Fujii, Fumihiko Ishimaru, Mitsune Tanimoto, Fusanori Nishimura, Shogo Takashiba

    SUPPORTIVE CARE IN CANCER   18 ( 3 )   395 - 398   2010年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    The commercial saliva substitute OralbalanceA (R) has been reported to alleviate symptoms of postradiotherapy xerostomia in head and neck cancer patients. OralbalanceA (R) may also be effective for xerostomia in patients undergoing hematopoietic cell transplantation (HCT) with high-dose chemotherapy and total-body irradiation. However, HCT patients are in a severely compromised condition, and saliva substitute must not promote infection. We reported previously that OralbalanceA (R) has antimicrobial effects against microbial species detected during HCT in vitro. This study was performed to determine the in vivo effects of OralbalanceA (R) on oral mucosal total bacterial counts in patients undergoing HCT.
    A total of 18 neutropenic patients undergoing HCT were enrolled in this study. Before and after 1 week of OralbalanceA (R) use, bacterial samples were obtained from patients by wiping an area of I center dot 1 cm on the buccal mucosa with sterilized cotton swabs. Total bacterial counts of the obtained samples were examined by quantitative polymerase chain reaction amplification of the bacterial 16S ribosomal RNA gene. As controls, bacterial samples were also obtained from ten healthy subjects, and total bacterial counts were examined.
    No significant increase in bacterial count was observed with use of OralbalanceA (R). None of the patients showed bacterial counts above the range found in healthy controls after using OralbalanceA (R).
    In neutropenic patients undergoing HCT, OralbalanceA (R) did not increase the total counts of oral mucosal bacteria beyond the range found in healthy controls. Oral care using OralbalanceA (R) may alleviate the symptoms induced by hyposalivation without promoting infection.

    DOI: 10.1007/s00520-009-0789-x

    Web of Science

    PubMed

    researchmap

  • Rapid detection of mecA and spa by the loop-mediated isothermal amplification (LAMP) method. 査読

    Y Koide, H Maeda, K Yamabe, Koji Naruishi, T Yamamoto, S Kokeguchi, S Takashiba

    Letters in Applied Microbiology   Vol.50 ( No.4 )   386 - 392   2010年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The LAMP offers an alternative detection assay for mecA and spa with a great advantage of the rapidity.

    DOI: 10.1111/j.1472-765X.2010.02806.x

    PubMed

    researchmap

  • Oral mucositis in patients receiving reduced-intensity regimens for allogeneic hematopoietic cell transplantation: comparison with conventional regimen 査読 国際誌

    Kanayo Takahashi, Yoshihiko Soga, Yumeno Murayama, Mika Udagawa, Hitomi Nishimoto, Yuko Sugiura, Yoshinobu Maeda, Mitsune Tanimoto, Shogo Takashiba

    SUPPORTIVE CARE IN CANCER   18 ( 1 )   115 - 119   2010年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Severe oral mucositis induced by allogeneic hematopoietic cell transplantation (HCT) is associated with intolerable pain and risk of systemic bacteremia infection. Differences between conventional HCT and reduced-intensity regimens for allogeneic HCT (RIST) may influence the occurrence and severity of oral mucositis. Here, we evaluated oral mucositis in patients undergoing RIST and compared the results with those in conventional allogeneic HCT patients to facilitate predictive measures for mucositis.
    A total of 127 consecutive patients undergoing HCT (conventional, 63; RIST, 64) were included in this study. Severity of oral mucositis during HCT period was evaluated daily. Differences in severity of mucositis among HCT types were analyzed. Use of morphine to control pain due to oral mucositis was evaluated in each HCT method.
    The severity of oral mucositis was reduced in patients undergoing RIST. Worsening of oral mucositis was delayed in patients receiving RIST. Use of morphine to control pain due to oral mucositis was significantly decreased in patients undergoing RIST compared with those receiving conventional allogeneic HCT.
    The severity of oral mucositis was reduced and the peak day of oral mucositis was delayed in RIST patients compared with those receiving conventional HCT.

    DOI: 10.1007/s00520-009-0637-z

    Web of Science

    PubMed

    researchmap

  • Genetic Risk Factors for Periodontitis in a Japanese Population 査読 国際誌

    T. Kobayashi, T. Nagata, S. Murakami, S. Takashiba, H. Kurihara, Y. Izumi, Y. Numabe, H. Watanabe, M. Kataoka, A. Nagai, J. Hayashi, H. Ohyama, Y. Okamatsu, Y. Inagaki, H. Tai, H. Yoshie

    JOURNAL OF DENTAL RESEARCH   88 ( 12 )   1137 - 1141   2009年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE PUBLICATIONS INC  

    Genetic variants at multiple loci have been shown to be associated with susceptibility to periodontitis. To better assess the genetic risk factors for periodontitis, we performed a case-control study in 319 Japanese individuals with periodontitis (172 aggressive and 147 chronic disease) and 303 race-matched healthy control individuals. Thirty-five functional gene polymorphisms that had been previously associated with immune responses were genotyped. For all gene polymorphisms tested, no significant differences were observed in the allele frequencies of persons with aggressive, chronic, and combined ( aggressive and chronic) periodontitis, compared with control individuals. Multiple logistic regression analysis revealed a significant association of the vitamin D receptor + 1056 T/C polymorphism with susceptibility to chronic periodontitis, after adjustment for age, gender, and smoking status (P = 0.002). These results suggest that none of the polymorphisms tested was strongly associated with periodontitis in a Japanese population. However, the vitamin D receptor + 1056 polymorphism may be related to chronic periodontitis.

    DOI: 10.1177/0022034509350037

    Web of Science

    PubMed

    researchmap

  • Porphyromonas gingivalis fimbriae-dependent interleukin-6 autocrine regulation by increase of gp130 in endothelial cells 査読 国際誌

    Y-S. Ho, M-T. Lai, S-J. Liu, C-T. Lin, K. Naruishi, S. Takashiba, H-H. Chou

    JOURNAL OF PERIODONTAL RESEARCH   44 ( 4 )   550 - 556   2009年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    Background and Objective:
    Local persistent infection by Porphyromonas gingivalis leads to inflammatory systemic diseases, such as atherosclerosis. We have reported previously that avirulent P. gingivalis fimbriae-dependent invasion into endothelial cells might be involved in progression of atherosclerosis. Although interleukin-6 (IL-6) regulates progression of atherosclerosis, little is known about the relationship of P. gingivalis fimbriae-dependent invasion to IL-6 regulation in endothelial cells.
    Material and Methods:
    We examined the secretion of IL-6 and the expression of the IL-6 signal transducer gp130 in human umbilical vein endothelial cells (HUVEC) infected with the wild-type FDC381 strain of P. gingivalisand a fimbriae-deficient mutant (fimA) by enzyme-linked immunosorbent assay, quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and flow cytometry (fluorescence-activated cell sorting, FACS) analysis.
    Results:
    Coculture of HUVEC with P. gingivalis resulted in increase of IL-6 secretion at 24 h postinfection. Interestingly, the increase was inhibited significantly in HUVEC infected with the P. gingivalis fimA mutant. In addition, the increase of IL-6 secretion induced by P. gingivalis infection was significantly impaired by the meiosis specific kinase 1 inhibitor, PD98059, or the nuclear factor kappa B inhibitor, Bay11-7082. Furthermore, we demonstrated that gp130 expression increased with P. gingivalis infection. Importantly, gp130 expression was significantly impaired by P gingivalis fimA mutant infection compared with wild-type P. gingivalis infection, as assessed by both quantitative RT-PCR and FACS analysis.
    Conclusion:
    Our findings indicate that P. gingivalis fimbriae are important factors in the autocrine regulation of IL-6, by increasing gp130 in endothelial cells.

    DOI: 10.1111/j.1600-0765.2008.01150.x

    Web of Science

    PubMed

    researchmap

  • Febrile neutropenia and periodontitis: lessons from a case periodontal treatment in the intervals between chemotherapy cycles for leukemia reduced febrile neutropenia 査読 国際誌

    Yoshihiko Soga, Yoshiko Yamasuji, Chieko Kudo, Kaori Matsuura-Yoshimoto, Kokoro Yamabe, Yuko Sugiura, Yoshinobu Maeda, Fumihiko Ishimaru, Mitsune Tanimoto, Fusanori Nishimura, Shogo Takashiba

    SUPPORTIVE CARE IN CANCER   17 ( 5 )   581 - 587   2009年5月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Oral and systemic infections arising from the oral cavity are significant problems in clinical management of patients undergoing leukemia treatment. However, there is significant disparity in the reported incidences of development of periodontal infections. Evidence is limited to those showing the systemic influence of periodontal infection in neutropenic patients. This study indicated an association between febrile neutropenia (FN) and periodontitis in a case in which periodontal treatment in the intervals between chemotherapy cycles reduced FN in subsequent courses of chemotherapy and hematopoietic transplantation (HCT).
    Periodontal treatment was performed in a 61-year-old man with advanced periodontitis, who received HCT following three cycles of chemotherapy. After recovery from neutropenia induced by initial chemotherapy, periodontal treatment was performed in each chemotherapy interval period. Following extraction of teeth with severe advanced periodontitis, all teeth were subjected to periodontal pocket curettage and root planning, which are common periodontal treatments to reduce periodontal pockets harboring anaerobic periodontal bacteria, before HCT.
    Periodontal treatment successfully reduced periodontal pockets from 4.1 +/- 1.5 mm to 3.0 +/- 0.6 mm, which was almost within the healthy range (&lt; 3.0 mm), before HCT. The frequency of FN decreased significantly with increasing cycles of chemotherapy, and decreases in FN corresponded to progress of periodontal treatment. Blood cultures obtained a total of 12 times throughout leukemia treatment were all negative.
    The observations reported here indicate the importance of periodontal treatment in clinical management of patients undergoing leukemia treatment to prevent FN, although all blood cultures were negative.

    DOI: 10.1007/s00520-008-0532-z

    Web of Science

    PubMed

    researchmap

  • Antibacterial effect of bactericide immobilized in resin matrix 査読 国際誌

    Naoko Namba, Yasuhiro Yoshida, Noriyuki Nagaoka, Seisuke Takashima, Kaori Matsuura-Yoshimoto, Hiroshi Maeda, Bart Van Meerbeek, Kazuomi Suzuki, Shogo Takashiba

    DENTAL MATERIALS   25 ( 4 )   424 - 430   2009年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI LTD  

    Objective. Biomaterials with anti-microbial properties are highly desirable in the oral cavity. Ideally, bactericidal molecules should be immobilized within the biomaterial to avoid unwanted side-effects against surrounding tissues. They may then however loose much of their antibacterial efficiency. The aim of this study was to investigate how much antibacterial effect an immobilized bactericidal molecule still has against oral bacteria.
    Methods. Experimental resins containing 0, 1 and 3% cetylpyridinium chloride (CPC) were polymerized, and the bacteriostatic and bactericidal effects against Streptococcus mutans were determined. Adherent S. mutans on HAp was quantitatively determined using FE-SEM and living cells of S. mutans were quantified using real-time RT-PCR. The amount of CPC released from the 0%-, 1%- and 3%-CPC resin sample into water was spectrometrically quantified using a UV-vis recording spectrophotometer.
    Results. UV spectrometry revealed that less than 0.11 ppm of CPC was released from the resin into water for all specimens, which is lower than the minimal concentration generally needed to inhibit biofilm formation. Growth of S. mutans was significantly inhibited on the surface of the 3%-CPC-containing resin coating, although no inhibitory effect was observed on bacteria that were not in contact with its surface. When immersed in water, the antibacterial capability of 3%-CPC resin lasted for 7 days, as compared to resin that did not contain CPC.
    Significance. These results demonstrated that the bactericidal molecule still possessed significant contact bacteriostatic activity when it was immobilized in the resin matrix. (C) 2008 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.dental.2008.08.012

    Web of Science

    PubMed

    researchmap

  • Assessment of Chromosome 19 for Genetic Association in Severe Chronic Periodontitis 査読 国際誌

    Koichi Tabeta, Yasuko Shimada, Hideaki Tai, Yuichi Ishihara, Toshihide Noguchi, Yoshihiko Soga, Shogo Takashiba, Genki Suzuki, Terukazu Kobayashi, Akira Oka, Tetsuo Kobayashi, Kazuhisa Yamazaki, Hidetoshi Inoko, Hirornasa Yoshie

    JOURNAL OF PERIODONTOLOGY   80 ( 4 )   663 - 671   2009年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    Background: A genome-association study is a powerful toot for analyzing small gene effects in complex diseases such as chronic periodontitis (CP), although the cost of analysis is prohibitive. We designed a study using the DNA pooling method, which could be a breakthrough in lowering such costs. This study was conducted to assess the genetic association in severe CP in a Japanese population.
    Methods: We adopted a DNA pooling method by genotyping 454 densely spaced microsatellite (MS) markers in chromosome 19 as a pilot study, with the possibility of future use in a whole-genome study. This can reduce the high cost and technical burden, which is generally unavoidable in a genomic association study. Pooled DNA samples from 300 case subjects, 300 control subjects, and 200 systemically healthy subjects were screened by genotyping MS markers. The case-control association in the candidate region was analyzed by individual typing of MS and single nucleotide polymorphisms (SNPs).
    Results: The single MS marker allele 17 of 1902G31 was isolated in association with severe CP (P = 0.0012 for 2 x 2; P&lt;0.046 for 2 x m, where m refers to the number of polymorphic alleles observed in a population). No other SNP or MS polymorphism hypothesized to affect biologic functions in the critical region was found in the linkage disequilibrium block analysis.
    Conclusions: We efficiently isolated the susceptible locus for severe CP in chromosome 19 and identified a useful marker to evaluate the risk for disease. This approach can be applied to a whole-genome study in severe CP. J Periodontol 2009;80:663-671.

    DOI: 10.1902/jop.2009.080516

    Web of Science

    PubMed

    researchmap

  • Human leukocyte histocompatibility antigen class II-induced cytokines from human gingival fibroblasts promote proliferation of human umbilical vein endothelial cells: potential association with enhanced angiogenesis in chronic periodontal inflammation 査読 国際誌

    Y. Okada, M. Meguro, H. Ohyama, S. Yoshizawa, K. Takeuchi-Hatanaka, N. Kato, S. Matsushita, S. Takashiba, F. Nishimura

    JOURNAL OF PERIODONTAL RESEARCH   44 ( 1 )   103 - 109   2009年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    The role of human leukocyte histocompatibility antigen (HLA) class II molecules on non-antigen-presenting cells has been a matter of controversy. We previously reported that HLA-II molecules on human gingival fibroblasts (GF) do not present antigens, but transduce signals into the cells, resulting in the expression of several cytokines, such as interleukin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1), regulated upon activation, normal T-cell expressed and secreted (RANTES) and IL-8. However, the exact role of these cytokines, as well as other cytokines which are potentially secreted from GF, in the pathogenesis of chronic periodontal inflammation is not fully understood. The aim of this study was to observe the effects of HLA-II-induced cytokines on the proliferation of human umbilical vein endothelial cells (HUVEC).
    Antibody-based cytokine-microarray analyses were performed to detect potential cytokines associated with angiogenesis. Next, cytokine productivity was confirmed by quantitative methods. Then, cell proliferation assay was performed to see whether these cytokines promoted the proliferation of HUVEC.
    Besides IL-6, MCP-1, RANTES and IL-8, growth-related gene product (GRO) was newly identified as an HLA-II-induced cytokine released from GF. This was confirmed by a quantitative method. Cell culture supernatant from HLA-II-stimulated GF cultures promoted the growth of HUVEC. Addition of anti-IL-8 neutralizing antibody, anti-CXC receptor (CXCR)1 antibody and anti-MCP-1 antibody inhibited the growth of HUVEC in a dose-dependent manner, while addition of anti-GRO alpha antibody did not.
    The HLA-II-induced IL-8, via CXCR1, as well as MCP-1 from GF, promotes endothelial cell proliferation, which is possibly associated with enhanced angiogenesis in chronic periodontal lesions.

    DOI: 10.1111/j.1600-0765.2008.01097.x

    Web of Science

    PubMed

    researchmap

  • IL-6/sIL-6R enhances cathepsin B and L production via caveolin-1-mediated JNK-AP-1 pathway in human gingival fibroblasts 査読 国際誌

    Tomoko Yamaguchi, Koji Naruishi, Hideo Arai, Fusanori Nishimura, Shogo Takashiba

    JOURNAL OF CELLULAR PHYSIOLOGY   217 ( 2 )   423 - 432   2008年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Interleukir (IL)-6 has an important role in inflammatory diseases. Lysosomal enzymes cathepsins are widely expressed as cysteine proteases regulating inflammatory process. Caveolin-1 (Cav-1) is a scaffolding/regulatory membrane protein that interacts with signaling molecules. In this study, we investigated the role of Cav-1 on (1) the productivity, and (2) the enzymatic activity of cathepsin B and L in human gingival fibroblasts (HGFs) treated with IL-6 in the presence of soluble form of IL-6 receptor (sIL-6R). At first, we established the siRNA-mediated Cav-1 down-regulating in vitro systems by transient transfection of Cav-1 siRNA. The siRNA-mediated Cav-1 down-regulated cells were treated with IL-6/sIL-6R for indicated times. Then, cell lysates were collected, and examined the IL-6-induced signaling pathway, cathepsin B and L production, and measurement of cathepsins activity. To investigate the cathepsin L activity, cathepsin-(B . L) activity was measured after pretreatment with CA-074Me, a specific inhibitor for cathepsin B. We found that IL-6/sIL-6R enhanced significantly both production and activity of cathepsin B and L in HGFs. Interestingly, IL-6-mediated phosphorylation of both p44/42 MAPK and JNK was dramatically suppressed in Cav-1 down-regulated HGFs treated with IL-6/sIL-6R. In addition, both production and activity of cathepsin B and L were also significantly suppressed. Importantly, we demonstrated that JNK inhibition, but not p44/42 MAPK inhibition, significantly diminished IL-6/sIL-6R-induced cathepsin B and L production. Taken together, we concluded that IL-6/sIL-6R enhances cathepsin B and L production via IL-6/sIL-6R-mediated Cav-1-JNK-AP-1 pathway in HGFs. Our findings indicate that Cav-1 might be a therapeutic target for IL-6-mediated tissue degradation in periodontitis.

    DOI: 10.1002/jcp.21517

    Web of Science

    PubMed

    CiNii Article

    researchmap

  • Evaluation of xerostomia in hematopoietic cell transplantation by a simple capacitance method device 査読 国際誌

    Yuko Sugiura, Yoshihiko Soga, Sachiko Nishide, Kotoe Kono, Kanayo Takahashi, Nobuharu Fujii, Fumihiko Ishimaru, Mitsune Tanimoto, Fusanori Nishimura, Shogo Takashiba

    SUPPORTIVE CARE IN CANCER   16 ( 10 )   1197 - 1200   2008年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Goals Hematopoietic cell transplantation (HCT) may lead to the development of xerostomia. However, there have been few reports of xerostomia in HCT patients based on objective data. We investigated moisture in the oral mucosa in patients undergoing HCT by the capacitance method using a convenient device, Moisture Checker for Mucus (R) (MCM; Life Co., Ltd., Saitama, Japan).
    Subjects and methods Thirty-six patients undergoing HCT at Okayama University Hospital of Medicine and Dentistry (Male=22, Female=14; age=41.6 +/- 16.2 years old) were enrolled in this study. Moisture in the oral mucosa was measured by MCM in accordance with the manufacturer&apos;s instructions. The results were obtained as MCM values (%), which are the weight percentage of water content in the oral mucosal epithelium. As controls, moisture of the oral mucosa was also examined in healthy volunteers (Male=27, Female=35; age=43.0 +/- 14.6 years old).
    Main results Throughout the examination period, MCM values were significantly lower in patients who underwent HCT than in controls. The degree of mucosal moisture in HCT patients showed wide interindividual differences.
    Conclusion The degree of mucosal moisture in HCT patients was low and showed wide interindividual differences. Evaluation of xerostomia using such a device may contribute to appropriate oral care with saliva substitute.

    DOI: 10.1007/s00520-008-0470-9

    Web of Science

    PubMed

    researchmap

  • Distribution of Archaea in Japanese patients with periodontitis and humoral immune response to the components 査読 国際誌

    Kokoro Yamabe, Hiroshi Maeda, Susumu Kokeguchi, Ichiro Tanimoto, Norihiro Sonoi, Susumu Asakawa, Shogo Takashiba

    FEMS MICROBIOLOGY LETTERS   287 ( 1 )   69 - 75   2008年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING  

    There is controversy regarding the existence of archaeal pathogens. Periodontitis is one of the human diseases in which Archaea have been suggested to have roles as pathogens. This study was performed to investigate the distribution of Archaea in Japanese patients with periodontitis and to examine the serum IgG responses to archaeal components. Subgingival plaque samples were collected from 111 periodontal pockets of 49 patients (17 with aggressive periodontitis and 32 with chronic periodontitis), and 30 subgingival plaque samples were collected from 17 healthy subjects. By PCR targeting the 16S rRNA genie, Archaea were detected in 15 plaque samples (13.5% of total samples) from 11 patients (29.4% of patients with aggressive periodontitis and 18.8% of patients with chronic periodontitis). Archaea Were detected mostly (14/15) in severe diseased sites (pocket depth &gt;= 6 mm), while no amplicons were observed in any samples from healthy controls. Sequence analysis, of the PCR products revealed that the majority of Archaea in periodontal pockets were a Methanobrevibacter oralis-like phylotype. Western immnoblotting detected IgG antibodies against M. oralis in eight of the 11 sera from patients. These results suggest the potential of Archaea (M. oralis) as an antigenic pathogen of periodontitis.

    DOI: 10.1111/j.1574-6968.2008.01304.x

    Web of Science

    PubMed

    CiNii Article

    researchmap

  • Rapid and simple detection of eight major periodontal pathogens by the loop-mediated isothermal amplification method 査読 国際誌

    Junko Miyagawa, Hiroshi Maeda, Toshimitsu Murauchi, Susumu Kokeguchi, Kokoro Yamabe, Ichiro Tanimoto, Fusanori Nishimura, Kazuhiro Fukui, Shogo Takashiba

    FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY   53 ( 3 )   314 - 321   2008年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Loop-mediated isothermal amplification (LAMP) was applied to develop a rapid and simple detection system for eight periodontal pathogens: Aggregatibacter (Actinobacillus) actinomycetemcomitans, Campylobacter rectus, Eikenella corrodens, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia, Treponema denticola and Tannerella forsythia. Primers were designed from the 16S ribosomal RNA gene for each pathogen, and the LAMP amplified the targets specifically and efficiently under isothermal condition at 64 degrees C. To simplify the manipulation of LAMP examination, boiled cells and intact cells suspended in phosphate-buffered saline (PBS) were tested as templates besides extracted DNA template. The detection limits were 1-10 cells per tube using extracted DNA template. However, LAMP methods using boiled cells and intact cells required 10-100 and 100-1000 cells per tube, respectively. LAMPs for A. actinomycetemcomitans, P. gingivalis and P. intermedia were then applied to clinical plaque samples, and the method demonstrated equal or higher sensitivity compared with the conventional real-time PCR method. These findings suggest the usefulness of the LAMP method for the rapid and simple microbiological diagnosis of periodontitis, and the possibility of LAMP examination without the DNA extraction step.

    DOI: 10.1111/j.1574-695X.2008.00417.x

    Web of Science

    PubMed

    researchmap

  • Periodontal Tissue Regeneration Using Fibroblast Growth Factor-2: Randomized Controlled Phase II Clinical Trial 査読 国際誌

    Masahiro Kitamura, Keisuke Nakashima, Yusuke Kowashi, Takeo Fujii, Hidetoshi Shimauchi, Takashi Sasano, Toshi Furuuchi, Mitsuo Fukuda, Toshihide Noguchi, Toshiaki Shibutani, Yukio Iwayama, Shogo Takashiba, Hidemi Kurihara, Masami Ninomiya, Jun-ichi Kido, Toshihiko Nagata, Takafumi Hamachi, Katsumasa Maeda, Yoshitaka Hara, Yuichi Izumi, Takao Hirofuji, Enyu Imai, Masatoshi Omae, Mitsuru Watanuki, Shinya Murakami

    PLOS ONE   3 ( 7 )   e2611   2008年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Background: The options for medical use of signaling molecules as stimulators of tissue regeneration are currently limited. Preclinical evidence suggests that fibroblast growth factor (FGF)-2 can promote periodontal regeneration. This study aimed to clarify the activity of FGF-2 in stimulating regeneration of periodontal tissue lost by periodontitis and to evaluate the safety of such stimulation.
    Methodology/Principal Findings: We used recombinant human FGF-2 with 3% hydroxypropylcellulose (HPC) as vehicle and conducted a randomized double-blinded controlled trial involving 13 facilities. Subjects comprised 74 patients displaying a 2- or 3-walled vertical bone defect as measured &gt;= 3 mm apical to the bone crest. Patients were randomly assigned to 4 groups: Group P, given HPC with no FGF-2; Group L, given HPC containing 0.03% FGF-2; Group M, given HPC containing 0.1% FGF-2; and Group H, given HPC containing 0.3% FGF-2. Each patient underwent flap operation during which we administered 200 mu L of the appropriate investigational drug to the bone defect. Before and for 36 weeks following administration, patients underwent periodontal tissue inspections and standardized radiography of the region under investigation. As a result, a significant difference (p = 0.021) in rate of increase in alveolar bone height was identified between Group P (23.92%) and Group H (58.62%) at 36 weeks. The linear increase in alveolar bone height at 36 weeks in Group P and H was 0.95 mm and 1.85 mm, respectively (p = 0.132). No serious adverse events attributable to the investigational drug were identified.
    Conclusions: Although no statistically significant differences were noted for gains in clinical attachment level and alveolar bone gain for FGF-2 groups versus Group P, the significant difference in rate of increase in alveolar bone height (p = 0.021) between Groups P and H at 36 weeks suggests that some efficacy could be expected from FGF-2 in stimulating regeneration of periodontal tissue in patients with periodontitis.

    DOI: 10.1371/journal.pone.0002611

    Web of Science

    PubMed

    researchmap

  • Antimicrobial effects of the saliva substitute, Oralbalance (R), against microorganisms from oral mucosa in the hematopoietic cell transplantation period 査読 国際誌

    Yuko Sugiura, Yoshihiko Soga, Ichiro Tanimoto, Susumu Kokeguchi, Sachiko Nishide, Kotoe Kono, Kanayo Takahashi, Nobuharu Fujii, Fumihiko Ishimaru, Mitsune Tanimoto, Kokoro Yamabe, Soichiro Tsutani, Fusanori Nishimura, Shogo Takashiba

    SUPPORTIVE CARE IN CANCER   16 ( 4 )   421 - 424   2008年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Goals The commercially available saliva substitute Oralbalance (R) has been reported to alleviate symptoms of post-radiotherapy xerostomia in head and neck cancer patients. Oralbalance (R) may also be effective for xerostomia in patients undergoing hematopoietic cell transplantation (HCT) with high-dose chemotherapy and total-body irradiation. However, HCT patients are severely compromised, and saliva substitute must therefore not promote infection. This study was performed to determine the effects of Oralbalance (R) on microbial species identified during HCT.
    Patients and methods Microbial identification of oral mucosa was performed in 28 patients undergoing HCT. The antimicrobial effects of Oralbalance (R) against bacteria and fungi detected in the HCT period were examined in vitro. Briefly, bacteria and fungi were spread on agar plates, and 0.1g of Oralbalance (R) gel was applied (about phi 1cm). After incubation at 37 degrees C for 24h, the presence of a transparent zone of inhibition around Oralbalance (R) was observed.
    Main results Not only bacterial species constituting normal flora of the oral mucosa but also those not usually constituting normal flora, e.g., coagulase-negative Staphylococcus, were detected. A transparent zone was observed around Oralbalance (R) in all bacterial species examined. No transparent zone was observed for Candida albicans, but growth was inhibited in the area where Oralbalance (R) was applied.
    Conclusions Oralbalance (R) does not facilitate increases in microorganisms in the HCT period. Oral care with Oralbalance (R) does not promote infection in patients undergoing HCT.

    DOI: 10.1007/s00520-007-0391-z

    Web of Science

    PubMed

    researchmap

  • Polymorphisms in the 5 ' flanking region of IL12RB2 are associated with susceptibility to periodontal diseases in the Japanese population 査読 国際誌

    Kazu Takeuchi-Hatanaka, Hideki Ohyama, Fusanori Nishimura, Nahoko Kato-Kogoe, Yoshihiko Soga, Sho Matsushita, Keiji Nakasho, Koji Yamanegi, Naoko Yamada, Nobuyuki Terada, Shogo Takashiba

    JOURNAL OF CLINICAL PERIODONTOLOGY   35 ( 4 )   317 - 323   2008年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING  

    Objectives: The expression of interleukin (IL)-12R beta 2 molecule is a crucial regulatory factor in the T-helper type (Th) 1 differentiation of T cells. To elucidate the role of the cell-mediated immune (CMI) response in the pathogenesis of periodontitis, Japanese periodontal patients were subjected to single nucleotide polymorphism (SNP) analyses of the 5' flanking region of IL12RB2, whose variants are frequently detected in lepromatous leprosy patients, in which the very weak cellular immune response is caused by low expression of IL-12R beta 2.
    Material and Methods: The gene polymorphisms of the 5' flanking region of IL12RB2 were examined in subjects with several types of periodontal disease and in healthy controls. Serum immunoglobulin (Ig) G antibody titres against periodontopathic bacteria were measured and compared in periodontal patients with and without variant alleles of IL12RB2.
    Results: The frequencies of variant alleles of IL12RB2 were significantly higher in aggressive periodontitis patients as compared with healthy controls or chronic periodontitis patients. Serum IgG titres against all periodontal bacteria examined in subjects carrying variant alleles were higher than those in subjects without variant alleles.
    Conclusion: IL-12R beta 2 SNPs could be useful as genetic markers to access the susceptibility of the general population to periodontal disease. Low CMI responses or high humoral responses are associated with the pathogenesis of inflammatory periodontal diseases.

    DOI: 10.1111/j.1600-051X.2008.01208.x

    Web of Science

    PubMed

    researchmap

  • Appearance of multidrug-resistant opportunistic bacteria on the gingiva during leukemia treatment 査読 国際誌

    Yoshihiko Soga, Takashi Saito, Fusanori Nishimura, Fumihiko Ishimaru, Junji Mineshiba, Fumi Mineshiba, Hirokazu Takaya, Hideaki Sato, Chieko Kudo, Susumu Kokeguchi, Nobuharu Fujii, Mitsune Tanimoto, Shogo Takashiba

    JOURNAL OF PERIODONTOLOGY   79 ( 1 )   181 - 186   2008年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    Background: Dentists generally recognize the importance of periodontal treatment inpatients with leukemia, with the most attention paid to preventing the development of odontogenic infection. For physicians, the worst type of infection is one caused by multidrug-resistant bacteria. Here, we report a patient with an abnormal increase in multidrug-resistant opportunistic bacteria in the gingiva during hematopoietic cell transplantation (HCT).
    Methods: A 53-year-old woman receiving HCT for leukemia had an insufficient blood cell count for invasive periodontal treatment before HCT. Even brushing caused difficulties with hemostasis. Therefore, frequent pocket irrigation and local minocycline administration were performed.
    Results: The multidrug-resistant opportunistic bacterium Stenotrophomonas maltophilia was detected first in phlegm 2 days before HCT, and it was detected in a gingival smear and a blood sample 7 and I I days after HCT, respectively. The patient developed sepsis on day I I and died 14 days after HCT. Frequent irrigation and local antibiotic application were ineffective against S. maltophilia on the gingiva. Inflammatory gingiva without scaling and root planing showed bleeding tendency, and this interfered with the eradication of this bacterium.
    Conclusions: The gingiva in patients undergoing leukemia treatment acts as sites of proliferation and reservoirs for multidrug-resistant opportunistic bacteria. Severe systemic infection by multidrug-resistant bacteria in such patients with leukemia also may involve the gingiva. To prevent abnormal increases in such bacteria on the gingiva, scaling and/or root planing before chemotherapy, which reduces bleeding on brushing during the neutropenic period caused by chemotherapy, may contribute to infection control in such patients, although it was impossible in this case.

    DOI: 10.1902/jop.2008.070205

    Web of Science

    PubMed

    researchmap

  • Focal adhesion kinase mediates human leukocyte histocompatibility antigen class II-induced signaling in gingival fibroblasts 査読 国際誌

    S. Yoshizawa, M. Meguro, H. Ohyama, K. Takeuchi-Hatanaka, S. Matsushita, S. Takashiba, F. Nishimura

    JOURNAL OF PERIODONTAL RESEARCH   42 ( 6 )   572 - 579   2007年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING  

    Background and Objective: The role of human leukocyte antigen class II molecules on nonantigen-presenting cells has been a matter of controversy. We previously reported that human leukocyte antigen class II molecules on human gingival fibroblasts do not present antigens, but transduce signals into the cells by making a complex with antigenic peptide T-cell receptor or by stimulating cell surface human leukocyte antigen-DR molecules with human leukocyte antigen-DR antibody (L243), which mimics the formation of the human leukocyte antigen class II-antigenic peptide T-cell receptor complex, resulting in the expression of several cytokines. The aim of this study was to detect human leukocyte antigen class II-associated molecules mediating human leukocyte antigen class II-induced signals into the cells.
    Material and Methods: Antibody-based protein-microarray analysis was performed to detect activated signaling molecules in gingival fibroblasts stimulated via human leukocyte antigen class II molecules. Then, we examined if these molecules structurally associate with human leukocyte antigen class II and actually transduce signals into the cells.
    Results: Stimulation of human leukocyte antigen class II on gingival fibroblasts by L243 resulted in enhanced phosphorylation of focal adhesion kinase. Focal adhesion kinase was co-immunoprecipitated with human leukocyte antigen-DR by L243. Stimulation of gingival fibroblasts with L243 induced phosphorylation of focal adhesion kinase. Luteolin, a putative focal adhesion kinase inhibitor, suppressed phosphorylation of focal adhesion kinase and dose dependently inhibited human leukocyte antigen class II-induced cytokine production.
    Conclusion: Focal adhesion kinase is structurally associated with human leukocyte antigen-DR and mediates human leukocyte antigen class II-induced signals in gingival fibroblasts.

    DOI: 10.1111/j.1600-0765.2007.00985.x

    Web of Science

    PubMed

    researchmap

  • Macrophage-adipocyte interaction: Marked interleukin-6 production by lipopolysaccharide 査読 国際誌

    Akiko Yamashita, Yoshihiko Soga, Yoshihiro Iwamoto, Sayuri Yoshizawa, Hirotaka Iwata, Susumu Kokeguchi, Shogo Takashiba, Fusanori Nishimura

    OBESITY   15 ( 11 )   2549 - 2552   2007年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Objective: Recent studies suggested macrophages were integrated in adipose tissues, interacting with adipocytes, thereby exacerbating inflammatory responses. Persistent low-grade infection by gram-negative bacteria appears to promote atherogenesis. We hypothesized a ligand for toll-like receptor 4 (TLR4), bacterial lipopolysaccharide (LPS), would further exaggerate macrophage-adipocyte interaction.
    Research Methods and Procedures: RAW264.7 macrophage cell line and differentiated 3T3-L1 preadipocytes were co-cultured using transwell system. As a control, each cell was cultured independently. After incubation of the cells with or without Escherichia coli LPS, tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 production was evaluated.
    Results: Co-culture of macrophages and adipocytes with low concentration of Escherichia coli LPS (1 ng/mL) markedly up-regulated IL-6 production (nearly 100-fold higher than that of adipocyte culture alone, p &lt; 0.01), whereas TNF-alpha production was not significantly influenced. This increase was partially inhibited by anti-TNF-alpha neutralizing antibody. Recombinant TNF-a and LPS synergistically upregulated IL-6 production in adipocytes. However, this increase did not reach the level of production observed in co-cultures stimulated with LPS.
    Discussion: A ligand for TLR-4 stimulates macrophages to produce TNF-alpha. TNF-alpha, thus produced, cooperatively upregulates IL-6 production with other soluble factors secreted either from adipocytes or macrophages in these cells. Markedly up-regulated IL-6 would greatly influence the pathophysiology of diabetes and its vascular complications.

    DOI: 10.1038/oby.2007.305

    Web of Science

    PubMed

    researchmap

  • cAMP-response element binding protein (CREB) regulates cyclosporine-A-mediated down-regulation of cathepsin B and L synthesis 査読 国際誌

    Kazuhiro Omori, Koji Naruishi, Tomoko Yamaguchi, Shun-Ai Li, Mayumi Yamaguchi-Morimoto, Kaori Matsuura, Hideo Arai, Kohji Takei, Shogo Takashiba

    CELL AND TISSUE RESEARCH   330 ( 1 )   75 - 82   2007年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Cyclosporin A (CsA) is an immunosuppressant with severe side effects including gingival overgrowth. We have previously reported that CsA impairs the activity of the lysosomal enzymes cathepsin B and L in human gingival fibroblasts (HGFs). Here, we have examined the effects of CsA on the DNA-binding activity of the cyclic AMP response element-binding protein (CREB) and cell viability, and the effects of CREB on cathepsin B and L synthesis and activity in HGFs. We have confirmed that CsA down-regulates cathepsin B and L synthesis. Further, CsA has no effect on cell viability and dramatically impairs CREB-DNA binding activity. Importantly, the synthesis of cathepsin B and L is down-regulated, and their activity is also significantly impaired in HGFs transfected with plasmid expressing dominant-negative CREB. These results suggest that CREB is essential for the CsA-mediated down-regulation of cathepsin B and L synthesis in HGFs.

    DOI: 10.1007/s00441-007-0457-8

    Web of Science

    PubMed

    researchmap

  • Relationship of periodontal infection to serum antibody levels to periodontopathic bacteria and inflammatory markers in periodontitis patients with coronary heart disease 査読 国際誌

    K. Yamazaki, T. Honda, H. Domon, T. Okui, K. Kajita, R. Amanuma, C. Kudoh, S. Takashiba, S. Kokeguchi, F. Nishimura, M. Kodama, Y. Aizawa, H. Oda

    CLINICAL AND EXPERIMENTAL IMMUNOLOGY   149 ( 3 )   445 - 452   2007年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING  

    Several reports have demonstrated a possible association of periodontal infections with coronary heart disease (CHD) by elevated antibody titre to periodontopathic bacteria in CHD patients compared with non-diseased controls. Although each periodontopathic bacterium may vary in virulence for periodontitis and atherosclerosis, antibody response to multiple bacteria in CHD patients has not been understood fully. Therefore, serum levels of antibody to 12 periodontopathic bacteria together with other atherosclerotic risk markers were compared among 51 patients with CHD, 55 patients with moderate to severe chronic periodontitis and 37 healthy individuals. The antibody response was the most prevalent for Porphyromonas gingivalis, a major causative organism, in CHD as well as periodontitis patients. However, antibody positivity was different between CHD and periodontitis if the response was analysed for two different strains of P. gingivalis, namely FDC381 and Su63. While periodontitis patients were positive for both P. gingivalis FDC381 and Su63, a high frequency of antibody positivity for P. gingivalis Su63 but not for FDC381 was observed in CHD patients. The results indicate that the presence of particular periodontopathic bacteria with high virulence may affect atherogenesis. Identifying the virulence factors of P. gingivalis Su63 may gain insight into the new therapeutic modality for infection-induced deterioration of atherosclerosis.

    DOI: 10.1111/j.1365-2249.2007.03450.x

    Web of Science

    PubMed

    researchmap

  • Gene profiles during root canal treatment in experimental rat periapical lesions 査読 国際誌

    Zulema Rosalia Arias Martinez, Koji Naruishi, Keisuke Yamashiro, Fumio Myokai, Teruo Yamada, Kaori Matsuura, Naoko Namba, Hideo Arai, Junzo Sasaki, Yoshimitsu Abiko, Shogo Takashiba

    JOURNAL OF ENDODONTICS   33 ( 8 )   936 - 943   2007年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    The purpose of this study was to profile gene expression in periapical lesions during root canal treatment (RCT). Periapical lesions were induced experimentally by exposing the pulp in Sprague-Dawley rats. After 3 wk, the animals received root canal filling (RCF) and were sacrificed 1 or 4 wk later. From the periapical tissues, total RNA was extracted and processed for cDNA-microarray analysis. The lesions were histologically and radiographically confirmed to expand 4 wk after pulp exposure (inflammation phase) and to stabilize 4 wk after RCF (healing phase). In approximately 30,000 genes on the microarray, 203 genes were up-regulated to more than 5-fold (e.g., IL-1 beta), and 864 genes were down-regulated to less than 20% of baseline level (e.g., caspase 8) in inflammation phase. Compared with inflammation phase, we found that 133 genes were up-regulated (e.g., IL-1 alpha) and 50 genes were down-regulated (e.g., defensin alpha 5) in healing phase. Corresponding to the gene expression profiles, accumulation of IL-1 alpha and IL-1 beta was observed in the periapical lesions by immunohistochemistry. These gene profiles might be useful in diagnosing the healing process of periapical lesions.

    DOI: 10.1016/j.joen.2007.04.016

    Web of Science

    PubMed

    researchmap

  • Oligonucleotide array analysis of cyclic tension-responsive genes in human periodontal ligament fibroblasts 査読 国際誌

    Keisuke Yamashiro, Fumio Myokai, Koichi Hiratsuka, Tadashi Yamamoto, Kyoko Senoo, Hideo Arai, Fusanori Nishimura, Yoshimitsu Abiko, Shogo Takashiba

    INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY   39 ( 5 )   910 - 921   2007年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Mechanical stress results in differential gene expression that is critical to convert the stimulus into biochemical signals. Under physiological stress such as occlusal force, human periodontal ligament fibroblasts (HPLF) are associated with homeostasis of periodontal tissues however the changes in response to mechanotransduction remain uncharacterized. We hypothesized that cyclic tension-responsive (CT) genes may be used to identify a set of fundamental pathways of mechanotransduction. Our goal was to catalogue CT genes in cultured HPLE HPLF were subjected to cyclic tension up to 16 h, and total RNA was isolated from both tension-loaded and static HPLE The oligonucleotide arrays analysis revealed significant changes of mRNA accumulation for 122 CT genes, and their kinetics were assigned by the K-means clustering methods. Ingenuity Pathway Analysis was completed for HPLF mechanotransduction using 50 CT genes. This analysis revealed that cyclic tension immediately down-regulated all nuclear transcription factors except v-fos FBJ murine osteosarcoma viral oncogene homolog (FOS) reacting as an early responsive gene. In turn, transcription factors such as tumor protein p53 binding protein 2 (TP53BP2), and extra-nuclear molecules such as adrenergic receptor beta 2 (ADRB2) were up-regulated after 1-2 h, which may result in fundamental HPLF functions to adapt to cyclic tension. Subsequent inhibition assays using Y27632, a pharmacologic inhibitor of Rho-associated kinase (ROCK), suggested that HPLF has both ROCK-dependent and ROCK-independent CT genes. Mechanical stress was found to effect the expression of numerous genes, in particular, expression of an early responsive gene; FOS initiates alteration of HPLF behaviors to control homeostasis of the periodontal ligament. (C) 2007 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.biocel.2007.01.015

    Web of Science

    PubMed

    researchmap

  • High glucose up-regulates lipopolysaccharide-stimulated inflammatory cytokine production via c-jun N-terminal kinase in the monocytic cell line THP-1 査読 国際誌

    Hirotaka Iwata, Yoshihiko Soga, Michio Meguro, Sayuri Yoshizawa, Yuka Okada, Yoshihiro Iwamoto, Akiko Yamashita, Shogo Takashiba, Fusanori Nishimura

    JOURNAL OF ENDOTOXIN RESEARCH   13 ( 4 )   227 - 234   2007年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE PUBLICATIONS LTD  

    Diabetic subjects are susceptible to atherosclerosis. It has been postulated that inflammation plays a crucial role in atherogenesis. Since previous studies suggested persistent low-grade infection by Gram-negative bacteria such as Chlamydia spp. and/or periodontal infection is associated with increased atherogenesis among diabetic subjects, we hypothesized that macrophages under hyperglycemia respond to lipopolysaccharide (LPS) challenge in a more exaggerated manner than under normal glucose conditions. Therefore, we examined cytokine productivity and associated signal transduction molecules in LPS-stimulated the monocytic cell line THP-1, under conditions of hyperglycemia. Differentiated THP-1 cells were cultured under normal and high glucose conditions without fetal bovine serum, and were stimulated with Escherichia coli LPS in the presence of LPS binding protein. Following stimulation, activated signal transduction molecules were detected by protein rnicroarray and confirmed thereafter. Results indicated that c-jun N-terminal kinase (INK) was highly-phosphorylated at high glucose concentrations, and this was confirmed by Western-immunoblotting. Tumor necrosis factor-alpha and monocyte chemo-attractant protein-1 production were significantly enhanced under these conditions. SP600125, a selective inhibitor of JNK, dose-dependently suppressed the production of these cytokine. Therefore, we suggest that this may be one of the mechanisms by which sub-clinical infection by Gram-negative bacteria promotes atherosclerosis in diabetic subjects.

    DOI: 10.1177/0968051907082608

    Web of Science

    PubMed

    researchmap

  • Regression of pustulosis palmaris et plantaris by periodontal treatment in a subject with severe periodontitis 査読 国際誌

    Hiroshi Akazawa, Fushanori Nishimura, Hiroshi Maeda, Shogo Takashiba, Atsushi Mine, Kenji Maekawa, Takuo Kuboki

    INTERNATIONAL JOURNAL OF DERMATOLOGY   45 ( 12 )   1420 - 1422   2006年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING  

    A 38-year-old woman with pustulosis palmaris et plantaris (PPP) was referred to the dentist with a suspicion of metal allergy (Fig. 1A). She had noticed the symptoms 3 years previously on her palms and legs. As her symptoms did not improve spontaneously, she visited her dermatologist. There, she was recorded as follows: (1) pustules localized only on the palms and soles; (2) no lesions with eczema or psoriasis on any other parts of the body; and (3) no family history of pustulosis. On the basis of these observations, she was diagnosed with PPP. She was treated with topical application of corticosteroids; however, her symptoms did not improve. Her dermatologist then suspected metal allergy and performed a patch test. Her skin reacted positively with chromium sulfate, zinc chloride, and mercury bichloride. Therefore, she was referred to our dental hospital for the investigation of the possible association of PPP with oral restorative materials.
    Her medical records were unremarkable, except for extremely severe periodontitis, which she was aware of more than 10 years previously; however, she had not received any comprehensive periodontal treatment. On presentation, we first analyzed the restorative materials of the teeth to determine whether they contained any positive elements detected by the patch test. Unfortunately, we could not detect any positive elements in the oral cavity. Her blood chemistry did not indicate any abnormalities. The white blood cell count was 5600/mm(3), with differential counts of 49.3% neutrophils, 28.0% lymphocytes, 7.6% monocytes, 14.4% eosinophils, and 0.7% basophils. C-reactive protein was not elevated, as measured by the conventional method. Immunoglobulin G (IgG), IgA, and IgM concentrations were all within the normal range (IgG, 1243.0 mg/dL; IgA, 131.6 mg/dL; IgM, 149.6 mg/dL). Because of these laboratory data, we first treated her periodontitis to determine whether there was a possible relationship of her PPP to severe periodontitis.
    Her periodontitis was very severe (Fig. 2A,B). The extraction of several teeth, as well as the correction of the shape of the inflamed alveolar bone (the bone supporting the teeth) to a physiological shape, was necessary. We first treated her periodontitis with the topical application of antibiotics (minocycline-HCl ointment) to determine the effect of anti-infectious periodontal treatment on PPP. We administered the antibiotics in every periodontal pocket once a week for a period of 1 month. One month after treatment, her symptoms appeared to improve slightly (Fig. 1B). We next performed surgical treatment, including teeth extractions and correction of the bone shape. Two to three days after surgery, her PPP symptoms worsened suddenly (Fig. 1C), and continued for up to 1 month (Fig. 1D). From 1 month after surgery, however, her symptoms gradually improved and complete remission was observed (Fig. 1E). In addition, although more than 2 years have passed since complete remission, no recurrence has been observed (Fig. 1F).

    DOI: 10.1111/j.1365-4632.2006.02900.x

    Web of Science

    PubMed

    researchmap

  • Cloning and characterization of lipopolysaccharide-induced tumor necrosis factor alpha factor promoter 査読 国際誌

    Nobuyuki Shiomi, Fumio Myokai, Koji Naruishi, Kosuke Oyaizu, Kyoko Senoo, Tomoko Yamaguchi, Salomon Amar, Shogo Takashiba

    FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY   47 ( 3 )   360 - 368   2006年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING  

    We have recently identified lipopolysaccharide tumor-induced tumor necrosis factor alpha factor (LITAF) as a novel transcription factor controlling necrosis factor (TNF)-alpha expression in the human monocytic cell line, THP-1. To characterize the human (h) LITAF promoter, we isolated a 1.2-kb DNA fragment and followed this by a screening of human genomic DNA with a hLITAF cDNA probe. A 34-bp sequence domain located from nucleotides -74 to -43 in the hLITAF promoter exhibited the highest basal reporter gene activity; however, the activity was not elevated by lipopolysaccharide (LPS) stimulation. The sequence domain included a consensus sequence for hepatocyte nuclear factor (HNF)-3 alpha, regulating the transcription of many kinds of genes. Interestingly, the DNA sequence position between -542 and -538 in the hLITAF promoter contained the CTCCC motif, which has been reported to act as a specific binding site for hLITAF protein. Electrophoretic mobility shift assays demonstrated that LPS induced the binding of THP-1 nuclear factors to a 22 bp probe containing the CTCCC motif. In addition, hLITAF mRNA and nuclear hLITAF protein increased significantly in the THP-1 cells immediately after LPS stimulation. These results suggest that the consensus sequence for HNF-3 alpha, or a nuclear binding protein to the CTCCC motif, may play an important role in regulating LPS-dependent LITAF transcription.

    DOI: 10.1111/j.1574-695X.2006.00094.x

    Web of Science

    PubMed

    researchmap

  • Identification of phosphorylation sites in N-linked glycans by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. 国際誌

    Minako Takashiba, Yasunori Chiba, Yoshifumi Jigami

    Analytical chemistry   78 ( 14 )   5208 - 13   2006年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Glycan phosphorylation is a significant feature of complex carbohydrate chemistry and glycobiology. For example, N-linked glycans containing mannose-6-phosphate (Man-6-P) residues play a key role as targeting signals for the transport of proteins from the Golgi apparatus to lysosomes. Structural information on Man-6-P glycans involved in transport of proteins is usually obtained using nuclear magnetic resonance (NMR) spectroscopy. However, an alternative and simple method with comparable accuracy is desirable because large amounts of samples and special techniques are required for structural analysis using NMR. Recently, postsource decay (PSD) fragment spectra obtained by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) have provided critical information on complex carbohydrates. Since few Man-6-P-containing glycans are commercially available, very little information has been collected on the mass spectrometry of phosphorylated glycans. In this report, four kinds of phosphorylated glycans labeled with 2-aminopyridine (PA) were purified from yeast mannan, and their PSD spectra were measured in the positive ion mode. The phospho-6-O-mannose monoester linkages (PO3H-Man) in glycans are stable, although cleavage of the mannose-1-phosphate linkage (Man-alpha-1-PO3H) occurs readily. Fragment ions indicated the presence of the alpha-1,3-branching chain of an N-linked high-mannose-type glycan, and characteristic fragmentation patterns were observed for phosphorylated glycans. On the basis of the MALDI-PSD spectra, we deduced fragmentation rules for phosphorylated N-glycans that will be valuable for distinguishing the position of phosphorylation.

    PubMed

    researchmap

  • Gene polymorphisms in periodontal health and disease 査読 国際誌

    S Takashiba, K Naruishi

    PERIODONTOLOGY 2000   40   94 - 106   2006年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING  

    DOI: 10.1111/j.1600-0757.2005.00142.x

    Web of Science

    PubMed

    researchmap

  • Anti-inflammatory effect of linear polarized infrared irradiation on interleukin-1 beta-induced chemokine production in MH7A rheumatoid synovial cells 査読 国際誌

    Y Shibata, N Ogura, K Yamashiro, S Takashiba, T Kondoh, K Miyazawa, M Matsui, Y Abiko

    LASERS IN MEDICAL SCIENCE   20 ( 3-4 )   109 - 113   2005年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER LONDON LTD  

    We examined the anti-inflammatory effect of infrared linear polarized light irradiation on the MH7A rheumatoid fibroblast-like synoviocytes (FLS) stimulated with the proinflammatory cytokine interleukin (IL)-1 beta. Expression of messenger ribonucleic acids (mRNAs) encoding IL-8, RANTES (regulated upon activation, normal T cell expressed and secreted), growth-related gene alpha (GRO alpha), and macrophage inflammatory protein-1 alpha (MIP1 alpha) was measured using real-time reverse transcription polymerase chain reaction, and the secreted proteins were measured in the conditioned media using enzyme-linked immunosorbent assays. We found that irradiation with linear polarized infrared light suppressed IL-1 beta-induced expression of IL-8 mRNA and, correspondingly, the synthesis and release of IL-8 protein in MH7A cells. This anti-inflammatory effect was equivalent to that obtained with the glucocorticoid dexamethasone. Likewise, irradiation suppressed the IL-1 beta-induced expression of RANTES and GRO alpha mRNA. These results suggest that the irradiation of the areas around the articular surfaces of joints affected by rheumatoid arthritis (RA) using linear polarized light may represent a useful new approach to treatment.

    DOI: 10.1007/s10103-005-0350-1

    Web of Science

    PubMed

    researchmap

  • alpha 2 integrin+807 polymorphism in drug-induced gingival overgrowth 査読 国際誌

    M Ogino, J Kido, M Bando, N Hayashi, C Wada, T Nagata, F Nishimura, Y Soga, S Takashiba, T Kubota, M Itagaki, Y Shimada, H Tai, H Yoshie, N Yamazaki, Y Shinohara, M Kataoka

    JOURNAL OF DENTAL RESEARCH   84 ( 12 )   1183 - 1186   2005年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

    alpha 2 integrin on fibroblasts is reported to play an important role in the induction of drug- induced gingival overgrowth, which is characterized by excessive accumulation of type I collagen in gingival connective tissue. Silent polymorphism 807 T/ C within the alpha 2 integrin gene is associated with high/ low alpha 2 integrin expression. The aim of this study was to test the hypothesis that expression of alpha 2 integrin 807 T/ C polymorphism correlates with drug- induced gingival overgrowth. A case- control study comparing 136 subjects taking calcium channel blockers ( 72 with vs. 64 without drug- induced gingival overgrowth) demonstrated that the frequency of the + 807 C allele was significantly higher in the case group than in the controls ( odds ratio, 3.61; 95% confidence interval, 2.14 - 6.10; P &lt; 0.05). The present findings suggest that the alpha 2 + 807 C allele is one of the genetic risk factors for drug- induced gingival overgrowth.

    DOI: 10.1177/154405910508401217

    Web of Science

    PubMed

    researchmap

  • alpha 2 integrin+807 polymorphism in drug-induced gingival overgrowth 査読

    M Ogino, J Kido, M Bando, N Hayashi, C Wada, T Nagata, F Nishimura, Y Soga, S Takashiba, T Kubota, M Itagaki, Y Shimada, H Tai, H Yoshie, N Yamazaki, Y Shinohara, M Kataoka

    JOURNAL OF DENTAL RESEARCH   84 ( 12 )   1183 - 1186   2005年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

    alpha 2 integrin on fibroblasts is reported to play an important role in the induction of drug- induced gingival overgrowth, which is characterized by excessive accumulation of type I collagen in gingival connective tissue. Silent polymorphism 807 T/ C within the alpha 2 integrin gene is associated with high/ low alpha 2 integrin expression. The aim of this study was to test the hypothesis that expression of alpha 2 integrin 807 T/ C polymorphism correlates with drug- induced gingival overgrowth. A case- control study comparing 136 subjects taking calcium channel blockers ( 72 with vs. 64 without drug- induced gingival overgrowth) demonstrated that the frequency of the + 807 C allele was significantly higher in the case group than in the controls ( odds ratio, 3.61; 95% confidence interval, 2.14 - 6.10; P &lt; 0.05). The present findings suggest that the alpha 2 + 807 C allele is one of the genetic risk factors for drug- induced gingival overgrowth.

    DOI: 10.1177/154405910508401217

    Web of Science

    researchmap

  • Isolation and expression of FIP-2 in wounded pulp of the rat 査読 国際誌

    M Oyama, F Myokai, T Ohira, N Shiomi, K Yamashiro, H Arai, F Nishimura, S Takashiba

    JOURNAL OF DENTAL RESEARCH   84 ( 9 )   842 - 847   2005年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

    Pulpal wound healing followed by cavity preparation may involve reactionary or reparative dentinogenesis in relation to the cavity position; however, little is known about the molecular responses. We aimed to isolate and analyze genes induced or suppressed in the wounded pulp to identify molecular processes involved in the pulp responses to injury. Twenty-three cDNAs were isolated by cDNA subtraction between healthy and wounded pulp of rats. By library screening, we identified rat 14.7K-interacting protein (rFIP)-2A and B genes homologous to human FIP-2, being involved in regulating membrane trafficking and cellular morphogenesis. RT-PCR analysis showed induction for only rFIP-2B in the wounded pulp. In situ hybridization analysis revealed that both rFIP-2s were expressed strongly in condensing mesenchymal cells of the palatal process and surrounding Meckel's cartilage, but not in intramembranous chondrogenic cells. Thus, upregulated rFIP-2B expression may play a role in regulating membrane trafficking or cellular morphogenesis of these embryonic and wounded pulpal cells.

    DOI: 10.1177/154405910508400912

    Web of Science

    PubMed

    researchmap

  • Isolation and expression of FIP-2 in wounded pulp of the rat 査読

    M Oyama, F Myokai, T Ohira, N Shiomi, K Yamashiro, H Arai, F Nishimura, S Takashiba

    JOURNAL OF DENTAL RESEARCH   84 ( 9 )   842 - 847   2005年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

    Pulpal wound healing followed by cavity preparation may involve reactionary or reparative dentinogenesis in relation to the cavity position; however, little is known about the molecular responses. We aimed to isolate and analyze genes induced or suppressed in the wounded pulp to identify molecular processes involved in the pulp responses to injury. Twenty-three cDNAs were isolated by cDNA subtraction between healthy and wounded pulp of rats. By library screening, we identified rat 14.7K-interacting protein (rFIP)-2A and B genes homologous to human FIP-2, being involved in regulating membrane trafficking and cellular morphogenesis. RT-PCR analysis showed induction for only rFIP-2B in the wounded pulp. In situ hybridization analysis revealed that both rFIP-2s were expressed strongly in condensing mesenchymal cells of the palatal process and surrounding Meckel's cartilage, but not in intramembranous chondrogenic cells. Thus, upregulated rFIP-2B expression may play a role in regulating membrane trafficking or cellular morphogenesis of these embryonic and wounded pulpal cells.

    DOI: 10.1177/154405910508400912

    Web of Science

    researchmap

  • Transcriptional regulation of beta-defensin-2 by lipopolysaccharide in cultured human cervical carcinoma (HeLa) cells 査読 国際誌

    J Mineshiba, F Myokai, F Mineshiba, K Matsuura, F Nishimura, S Takashiba

    FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY   45 ( 1 )   37 - 44   2005年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Human beta-defensin-2 (hBD-2) is an antimicrobial peptide with a broad spectrum of antimicrobial activity against bacteria, yeast and fungi. Here, we analyzed the transcriptional regulation of hBD-2 in cultured human cervical carcinoma (HeLa) cells with or without lipopolysaccharide (LPS). DNA from position -329 to -39 in the hBD-2 promoter region contained the consensus binding sites for transcription factors, one site for nuclear factor for IL-6 expression (NF-IL6) and two sites for nuclear factor-kappa B (NF-kappa B). Reporter gene assays for promoter activity revealed that the region had the highest level of responsiveness to LPS. Furthermore, mutations in both of the NF-kappa B binding sites caused a significant reduction of the responsiveness to LPS, whereas mutation in the NF-IL6 binding site resulted in an elevation of the basal promoter activity. Electrophoretic mobility shift assays demonstrated that LPS induced the binding of HeLa nuclear factors to 60-bp probe containing the two NF-kappa B binding sites, suggesting that the sites were essential for the binding. Our results suggest that the two NF-kappa B binding sites contribute to LPS-mediated hBD-2 transcription while the NF-IL6 binding site represses LPS-independent hBD-2 transcription in the HeLa cells. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.femsim.2005.01.008

    Web of Science

    PubMed

    researchmap

  • Periodontal treatment in severe aplastic anemia 査読 国際誌

    K Oyaizu, F Mineshiba, J Mineshiba, H Takaya, F Nishimura, Tanimoto, I, H Arai, S Takashiba

    JOURNAL OF PERIODONTOLOGY   76 ( 7 )   1211 - 1216   2005年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    Background: Aplastic anemia (AA) is a rare hematologic disease characterized by hypo-cellular bone marrow. The clinical features include fatigue, increased bruising, and gingival bleeding caused by anemia, leukopenia, and thrombocytopenia. A patient with AA is at high risk for infection because of leukopenia. The risk of systemic infection is especially high in AA patients with severe local infections, including periodontitis. Accordingly, periodontal treatment should include antibiotic prophylaxis to reduce the risk of systemic infection. However, treatment of periodontitis in the AA patient is significantly complicated by the bleeding disorder. We present a case report of the successful periodontal treatment of an AA patient with spontaneous gingival bleeding.
    Methods: The patient was closely monitored for platelet and neutrophil counts before every treatment. The patient's platelet count was always under 10,000/mu l. Therefore, it was necessary to increase platelet counts to over 25,000/mu l by transfusion, after which subgingival scaling with anesthesia was performed. When the neutrophil count was less than 2,000/mu l, local minocycline chemotherapy was applied to the pockets. Periodontal infection was monitored by detection of bacterial DNA and measurement of serum immunoglobulin (Ig) G titer against periodontal bacteria.
    Results: Following the physical and chemical treatment, the gingival appearance improved dramatically and the spontaneous gingival bleeding disappeared. Moreover, the IgG titer against periodontal bacteria decreased to normal range and specific periodontal pathogens were no longer detectable in the tested pockets.
    Conclusion: We believe that the treatment strategy in the present report provides new sight into treatment planning for severely medically compromised patients.

    DOI: 10.1902/jop.2005.76.7.1211

    Web of Science

    PubMed

    researchmap

  • Periodontal treatment in severe aplastic anemia 査読

    K Oyaizu, F Mineshiba, J Mineshiba, H Takaya, F Nishimura, Tanimoto, I, H Arai, S Takashiba

    JOURNAL OF PERIODONTOLOGY   76 ( 7 )   1211 - 1216   2005年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    Background: Aplastic anemia (AA) is a rare hematologic disease characterized by hypo-cellular bone marrow. The clinical features include fatigue, increased bruising, and gingival bleeding caused by anemia, leukopenia, and thrombocytopenia. A patient with AA is at high risk for infection because of leukopenia. The risk of systemic infection is especially high in AA patients with severe local infections, including periodontitis. Accordingly, periodontal treatment should include antibiotic prophylaxis to reduce the risk of systemic infection. However, treatment of periodontitis in the AA patient is significantly complicated by the bleeding disorder. We present a case report of the successful periodontal treatment of an AA patient with spontaneous gingival bleeding.
    Methods: The patient was closely monitored for platelet and neutrophil counts before every treatment. The patient's platelet count was always under 10,000/mu l. Therefore, it was necessary to increase platelet counts to over 25,000/mu l by transfusion, after which subgingival scaling with anesthesia was performed. When the neutrophil count was less than 2,000/mu l, local minocycline chemotherapy was applied to the pockets. Periodontal infection was monitored by detection of bacterial DNA and measurement of serum immunoglobulin (Ig) G titer against periodontal bacteria.
    Results: Following the physical and chemical treatment, the gingival appearance improved dramatically and the spontaneous gingival bleeding disappeared. Moreover, the IgG titer against periodontal bacteria decreased to normal range and specific periodontal pathogens were no longer detectable in the tested pockets.
    Conclusion: We believe that the treatment strategy in the present report provides new sight into treatment planning for severely medically compromised patients.

    DOI: 10.1902/jop.2005.76.7.1211

    Web of Science

    researchmap

  • Role of helper T cells in the humoral immune responses against 53-kDa outer membrane protein from Porphyromonas gingivalis 査読 国際誌

    N Kato, H Ohyama, F Nishimura, S Matsushita, S Takashiba, Y Murayama

    ORAL MICROBIOLOGY AND IMMUNOLOGY   20 ( 2 )   112 - 117   2005年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL MUNKSGAARD  

    Outer membrane protein with a 53-kDa molecular weight (Ag53) isolated from Porphyromonas gingivalis evokes strong humoral immune responses in many periodontitis patients. To examine the effects of cytokines produced by Ag53-specific Th cells on the IgG production against Ag53, we established Ag53-specific Th-cell lines from patients with early onset periodontitis and from healthy volunteers. We then developed a mixed lymphocyte culture system between Ag53-specific Th cells and auto- or allo-derived T-cell-depleted leukocytes produced from the subjects whose HLA class II haplotypes were completely matched. Interferon-gamma production was observed in all Th cell lines from patients and healthy subjects. As for Th2 type cytokines, interleukin (IL)-4, IL-5, IL-6 and IL-10 production varied greatly in Th cells regardless of the periodontal condition of the donor. Only Th cell lines with a high Th2/Th1 ratio induced Ag53-specific IgG production when cocultured with T-cell-depleted leukocytes. Thus, the difference in Th2/Th1 balance may regulate the Ag53-specific IgG production.

    DOI: 10.1111/j.1399-302X.2004.00203.x

    Web of Science

    PubMed

    researchmap

  • Thiazolidinedione (pioglitazone) blocks P-gingivalis- and F-nucleatum, but not E-coli, lipopolysaccharide (LPS)-induced interleukin-6 (IL-6) production in adipocytes 査読

    M Yamaguchi, F Nishimura, H Naruishi, Y Soga, S Kokeguchi, S Takashiba

    JOURNAL OF DENTAL RESEARCH   84 ( 3 )   240 - 244   2005年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

    An elevated level of C-reactive protein (CRP) predicts the future development of coronary heart disease. Periodontitis appears to up-regulate CRP. CRP is produced by hepatocytes in response to interleukin-6 (IL-6). A major source of IL-6 in obese subjects is adipocytes. We hypothesized that lipopolysaccharide (LPS) from periodontal pathogens stimulated adipocytes to produce IL-6, and that the production was suppressed by the drugs targeted against insulin resistance, thiazolidinedione ( pioglitazone), since this agent potentially showed an anti-inflammatory effect. Mouse 3T3-L1 adipocytes were stimulated with E. coli, P. gingivalis, and F. nucleatum LPS. The IL-6 concentration in culture supernatants was measured. All LPS stimulated adipocytes to produce IL-6. Although pioglitazone changed adipocyte appearance from large to small, and completely suppressed P. gingivalis and F. nucleatum LPS-induced IL-6 production, E. coli LPS-induced IL-6 production was not efficiently blocked. Thus, pioglitazone completely blocked periodontal-bacteria-derived LPS-induced IL-6 production in adipocytes, a major inducer of CRP.

    Web of Science

    researchmap

  • Thiazolidinedione (pioglitazone) blocks P-gingivalis- and F-nucleatum, but not E-coli, lipopolysaccharide (LPS)-induced interleukin-6 (IL-6) production in adipocytes 査読 国際誌

    M Yamaguchi, F Nishimura, H Naruishi, Y Soga, S Kokeguchi, S Takashiba

    JOURNAL OF DENTAL RESEARCH   84 ( 3 )   240 - 244   2005年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

    An elevated level of C-reactive protein (CRP) predicts the future development of coronary heart disease. Periodontitis appears to up-regulate CRP. CRP is produced by hepatocytes in response to interleukin-6 (IL-6). A major source of IL-6 in obese subjects is adipocytes. We hypothesized that lipopolysaccharide (LPS) from periodontal pathogens stimulated adipocytes to produce IL-6, and that the production was suppressed by the drugs targeted against insulin resistance, thiazolidinedione ( pioglitazone), since this agent potentially showed an anti-inflammatory effect. Mouse 3T3-L1 adipocytes were stimulated with E. coli, P. gingivalis, and F. nucleatum LPS. The IL-6 concentration in culture supernatants was measured. All LPS stimulated adipocytes to produce IL-6. Although pioglitazone changed adipocyte appearance from large to small, and completely suppressed P. gingivalis and F. nucleatum LPS-induced IL-6 production, E. coli LPS-induced IL-6 production was not efficiently blocked. Thus, pioglitazone completely blocked periodontal-bacteria-derived LPS-induced IL-6 production in adipocytes, a major inducer of CRP.

    Web of Science

    PubMed

    researchmap

  • Detection of periodontal pathogen Porphyromonas gingivalis by loop-mediated isothermal amplification method 査読 国際誌

    H Maeda, S Kokeguchi, C Fujimoto, Tanimoto, I, W Yoshizumi, F Nishimura, S Takashiba

    FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY   43 ( 2 )   233 - 239   2005年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    A method for nucleic acid amplification, loop-mediated isothermal amplification (LAMP) was employed to develop a rapid and simple detection system for periodontal pathogen, Porphyromonas gingivalis. A set of six primers was designed by targeting the 16S ribosomal RNA gene. By the detection system, target DNA was amplified and visualized on agarose gel within 30 min under isothermal condition at 64degreesC with a detection limit of 20 cells of P. gingivalis. Without gel electrophoresis, the LAMP amplicon was directly visualized in the reaction tube by addition of SYBR Green I for a naked-eye inspection. The LAMP reaction was also assessed by white turbidity of magnesium pyrophosphate (a by-product of LAMP) in the tube. Detection limits of these naked-eye inspections were 20 cells and 200 cells, respectively. Although false-positive DNA amplification was observed from more than 107 cells of Porphyromonas endodontalis, no amplification was observed in other five related oral pathogens. Further, quantitative detection of P. gingivalis was accomplished by a real-time monitoring of the LAMP reaction using SYBR Green I with linearity over a range of 10(2)-10(6) cells. The real-time LAMP was then applied to clinical samples of dental plaque and demonstrated almost identical results to the conventional real-time PCR with an advantage of rapidity. These findings indicate the potential usefulness of LAMP for detecting and quantifying P. gingivalis, especially in its rapidity and simplicity. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.femsim.2004.08.005

    Web of Science

    PubMed

    researchmap

  • Long-term cyclosporin A exposure suppresses cathepsin-B and -L activity in gingival fibroblasts 査読 国際誌

    M Yamaguchi, K Naruishi, H Yamada-Naruishi, K Omori, F Nishimura, S Takashiba

    JOURNAL OF PERIODONTAL RESEARCH   39 ( 5 )   320 - 326   2004年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL MUNKSGAARD  

    Background: Gingival overgrowth is a common side-effect following administration of cyclosporin A. We reported previously that lysosomal protease cathepsin-L activity, but not cathepsin-B, was significantly suppressed by short-term cyclosporin A exposure in human gingival fibroblasts. Although this suppression may lead to decreased degradation of gingival connective tissue, a net increase in matrix proteins, and gingival overgrowth, the effects of cyclosporin A need to be more elucidated, considering the long-term use for patients following organ transplantation.
    Objective: The aim of the present study was to evaluate the effects of clinically relevant doses of cyclosporin A on cultured human gingival fibroblasts. We evaluated the effects of long-term cyclosporin A exposure on cell proliferation, mRNA expression of various proteases and both cathepsin-B and -L activity in human gingival fibroblasts.
    Materials and Methods: Human gingival fibroblasts were isolated from three donors' healthy gingiva and cultured from five to eight passages with or without 200 ng/ml of cyclosporin A. Proliferative activity of cyclosporin A-treated cells was examined using MTT assay. Total RNA and cellular proteins were collected for semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis and for measurement of the cathepsin-B and -L activity.
    Results: Long-term cyclosporin A exposure had no effects on cell proliferation. Accumulation of cathepsin-B, -H and -L mRNA was markedly suppressed by long-term cyclosporin A exposure, whereas accumulation of another lysosomal enzyme N-acetyl-beta-d-glucosaminidase mRNA, which is involved in remodeling of gingival epithelium, was not apparently impaired in cyclosporin A-treated cells. Accumulation of matrix metalloprotease-1 (MMP-1) and tissue inhibitor of matrix metalloprotease-1 (TIMP-1) mRNA, which are involved in remodeling of extracellular matrix, also was not impaired. In addition, we demonstrated that long-term cyclosporin A exposure significantly suppressed not only the activity of the active form of cathepsin-(B + L) compared to the activity in non-treated cells (p = 0.0458), but also the activity of the active form of cathepsin-B (p &lt; 0.0001) in human gingival fibroblasts.
    Conclusion: The decreased ability of protein degradation by not only cathepsin-L but also cathepsin-B is, at least, one of the several factors developing the cyclosporin A-induced gingival overgrowth.

    DOI: 10.1111/j.1600-0765.2004.00746.x

    Web of Science

    PubMed

    researchmap

  • Identification of genes differentially regulated in rat alveolar bone wound healing by subtractive hybridization 査読

    T Ohira, F Myokai, N Shiomi, K Yamashiro, T Yamamoto, Y Murayama, H Arai, F Nishimura, S Takashiba

    JOURNAL OF DENTAL RESEARCH   83 ( 7 )   546 - 551   2004年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

    Periodontal healing requires the participation of regulatory molecules, cells, and scaffold or matrix. Here, we hypothesized that a certain set of genes is expressed in alveolar bone wound healing. Reciprocal subtraction gave 400 clones from the injured alveolar bone of Wistar rats. Identification of 34 genes and analysis of their expression in injured tissue revealed several clusters of unique gene regulation patterns, including the up-regulation at 1 wk of cytochrome c oxidase regulating electron transfer and energy metabolism, presumably occurring at the site of inflammation; up-regulation at 2.5 wks of pro-alpha-2 type I collagen involving the formation of a connective tissue structure; and up-regulation at 1 and 2 wks and down-regulation at 2.5 and 4 wks of ubiquitin carboxyl-terminal hydrolase 13 involving cell cycle, DNA repair, and stress response. The differential expression of genes may be associated with the processes of inflammation, wound contraction, and formation of a connective tissue structure.

    Web of Science

    researchmap

  • Identification of genes differentially regulated in rat alveolar bone wound healing by subtractive hybridization 査読 国際誌

    T Ohira, F Myokai, N Shiomi, K Yamashiro, T Yamamoto, Y Murayama, H Arai, F Nishimura, S Takashiba

    JOURNAL OF DENTAL RESEARCH   83 ( 7 )   546 - 551   2004年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

    Periodontal healing requires the participation of regulatory molecules, cells, and scaffold or matrix. Here, we hypothesized that a certain set of genes is expressed in alveolar bone wound healing. Reciprocal subtraction gave 400 clones from the injured alveolar bone of Wistar rats. Identification of 34 genes and analysis of their expression in injured tissue revealed several clusters of unique gene regulation patterns, including the up-regulation at 1 wk of cytochrome c oxidase regulating electron transfer and energy metabolism, presumably occurring at the site of inflammation; up-regulation at 2.5 wks of pro-alpha-2 type I collagen involving the formation of a connective tissue structure; and up-regulation at 1 and 2 wks and down-regulation at 2.5 and 4 wks of ubiquitin carboxyl-terminal hydrolase 13 involving cell cycle, DNA repair, and stress response. The differential expression of genes may be associated with the processes of inflammation, wound contraction, and formation of a connective tissue structure.

    Web of Science

    PubMed

    researchmap

  • High glucose enhances interleukin-6-induced vascular endothelial growth factor 165 expression via activation of Gp130-mediated p44/42 MAPK-CCAAT/enhancer binding protein signaling in gingival fibroblasts 査読 国際誌

    K Omori, K Naruishi, F Nishimura, H Yamada-Naruishi, S Takashiba

    JOURNAL OF BIOLOGICAL CHEMISTRY   279 ( 8 )   6643 - 6649   2004年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC  

    Diabetic patients are susceptible to severe inflammatory periodontitis manifesting as swollen gingiva with bleeding, but the underlying mechanism is not well understood. Our purpose was to determine the effect of a high glucose (HG) condition on the interleukin-6/soluble interleukin-6 receptor (IL-6/sIL-6R)-induced activation of signaling and vascular endothelial growth factor (VEGF) expression in human gingival fibroblasts (HGFs). In this study, HGFs were cultured for at least two passages under a normal glucose (NG; 5.5 mm) condition or high glucose (25 mm) condition. Importantly, the HG condition significantly induced expression of gp130 mRNA in HGFs compared with levels in control cells. Consistent with the expression of its mRNA, the HG condition also increased the expression of gp130 protein, and phosphorylation of the tyrosine residue by gp130 was enhanced significantly by IL-6/sIL-6R stimulation. Furthermore, the HG condition enhanced the IL-6/sIL-6R-induced phosphorylation of p44/42 MAPK and led to subsequent activation of CCAAT/enhancer binding protein in nuclei. In contrast, there was no significant difference in phosphorylation of JNK between the HG and NG condition. Interestingly, HGFs increased IL-6/sIL-6R,induced VEGF165 mRNA expression and VEGF165 secretion under the HG condition compared with levels under the NG condition. In contrast, the induction of VEGF165 secretion was partially inhibited by PD98059 (selective p44/42 MAPK inhibitor) under the HG condition. In addition, the VEGF165 secretion was completely inhibited by the combination of PD98059 and SP600125 (JNK inhibitor). Our findings suggest that the HG condition indirectly increases VEGF expression via activation of gp130-mediated p44/42 MAPK-CCAAT/enhancer binding protein signaling in HGFs. Thus, elevated VEGF secretion in HGFs under the HG condition may play a role in the development of the severe periodontitis observed in diabetic patients.

    DOI: 10.1074/jbc.M311688200

    Web of Science

    PubMed

    CiNii Article

    researchmap

  • Systemic up-regulation of sTNFR2 and IL-6 in Porphyromonas gingivalis pneumonia in mice 査読 国際誌

    M Petelin, K Naruishi, N Shiomi, J Mineshiba, H Arai, F Nishimura, S Takashiba, Y Murayama

    EXPERIMENTAL AND MOLECULAR PATHOLOGY   76 ( 1 )   76 - 81   2004年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Aspiration pneumonia is a common cause of death in older people, and the pathophysiology is a chronic respiratory failure with a mild airway inflammation. In this study, we established a mild inflammatory pneumonia model using Porphyromonas gingivalis (Pg) pathogen-infected mice. It elucidated the effects of Pg-infected pneumonia on proinflammatory cytokines tumor necrosis factor (TNF)-alpha, interleukin-6 (IL-6), and IL-1beta production in both lung tissue and serum. We also elucidated production of soluble (s) TNF receptor (R) s, because TNF-alpha is considered to be a dominant inflammatory mediator. Lung TNF-alpha levels significantly increased at 2 h after infection, and rapidly returned to basal level at 24 h. Consistent with increase of TNF-alpha, remarkable increase of sTNFR2 but not sTNFR1 was detected in lung tissue from 2 to 72 h. Interestingly, sTNFR2/sTNFR1 ratio was significantly enhanced at 2 h in serum. In addition, lung IL-1beta and IL-6 levels also significantly increased from 2 to 24 h. Importantly, we found that IL-6 levels in serum reflected its local level. These results may suggest that systemically produced sTNFR2 and IL-6 could be a key role to modulate proinflammatory activities of TNF-alpha in Pg-induced lung inflammation simulated aspiration pneumonia. (C) 2003 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.yexmp.2003.09.002

    Web of Science

    PubMed

    researchmap

  • CYP2C polymorphisms, phenytoin metabolism and gingival overgrowth in epileptic subjects 査読 国際誌

    Y Soga, F Nishimura, Y Ohtsuka, H Araki, Y Iwamoto, H Naruishi, N Shiomi, Y Kobayashi, S Takashiba, K Shimizu, Y Gomita, E Oka

    LIFE SCIENCES   74 ( 7 )   827 - 834   2004年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Previous studies suggested that the onset of phenytoin-induced gingival overgrowth depended on serum phenytoin concentration. Cytochrome P450 2C (CYP2C) plays an important role in phenytoin metabolism. Recently, single nucleotide polymorphisms in the coding region of CYP 2C influencing phenytoin metabolism were identified. The purpose of the present study was to see if CYP 2C polymorphisms might relate to the onset and severity of phenytoin-induced gingival overgrowth. Twenty-eight epileptic patients taking phenytoin aged 15 to 75 (mean age: 42.2 years old, 20 males and 8 females) and 56 unrelated healthy subjects aged 30 to 48 (mean age: 36.8 years old, 48 males and 8 females) were examined for CYP 2C polymorphisms. All epileptic subjects were examined for the degree of gingival overgrowth, daily phenytoin dose and serum phenytoin concentration. The results indicated about 7% of the subjects including epileptic and healthy subjects examined were positive for CYP 2C9*3. However, the degree of gingival overgrowth did not directly correlate with CYP 2C polymorphisms. Nevertheless, the subjects with severer gingival overgrowth exhibited significantly higher serum phenytoin concentration, indicating that phenytoin metabolism is an important determinant for the severity of the disease. Additionally, CYP 2C9*3 carriers exhibited significantly higher serum drug concentration to drug dose. Therefore, we concluded although the gene analysis is not directly related to diagnose the disease itself, it can be utilized in estimating serum phenytoin concentration from drug dose, which in turn serves to predict the future development and clinical course of the disease. (C) 2003 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.lfs.2003.07.018

    Web of Science

    PubMed

    CiNii Article

    researchmap

  • C-jun N-terminal kinase (JNK) interleukin SP600125, blocks interleukin (IL)-6-induced vascular endothelial growth factor (VEGF) production: Cyclosporine A partially mimics this inhibitory effect 査読 国際誌

    K Naruishi, F Nishimura, H Yamada-Naruishi, K Omori, M Yamaguchi, S Takashiba

    TRANSPLANTATION   76 ( 9 )   1380 - 1382   2003年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

    Angiogenesis is a common complication of organtransplant rejection. One of the primary responsible molecules for enhanced angiogenesis is vascular endothelial growth factor (VEGF). Activated protein (AP)-1 is considered to play a key role in the transcription of VEGF. c-jun N-terminal kinase (JNK), one of the MAP kinase family members, plays a critical role in AP-1 activation. Thus, we tested the effect of a novel JNK inhibitor, SP600125, on VEGF production in fibroblasts. SP600125 significantly suppressed interleukin (IL)-6-induced production of VEGF in cultured fibroblasts. Cyclosporine A (CsA), a known in vitro antiangiogenic reagent, partially mimicked this suppression. In fact, CsA suppressed IL-6-induced phosphorylation of JNK. The results indicate that although both SP600125 and CsA are anti-angiogenic by inhibiting VEGF production by way of a JNK-dependent pathway, the inhibitory effect was much stronger with the novel inhibitor of JNK than with CsA.

    DOI: 10.1097/01.TP.0000085661.52980.95

    Web of Science

    PubMed

    researchmap

  • Quantitative real-time PCR using TaqMan and SYBR Green for Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, tetQ gene and total bacteria 査読 国際誌

    H Maeda, C Fujimoto, Y Haruki, T Maeda, S Kokeguchi, M Petelin, H Arai, Tanimoto, I, F Nishimura, S Takashiba

    FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY   39 ( 1 )   81 - 86   2003年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Accurate quantification of bacterial species in dental plaque is needed for microbiological diagnosis of periodontal diseases. The present study was designed to assess the sensitivity, specificity and quantitativity of the real-time PCR using the GeneAmp(R) Sequence Detection System with two fluorescence chemistries. TaqMan probe with reporter and quencher dye, and SYBR Green dye were used for sources of the fluorescence. Primers and probes were designed for Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia and total bacteria based on the nucleotide sequences of the respective 16S ribosomal RNA genes. Since spread of antibiotic resistance genes is one of the crucial problems in periodontal therapy, quantitative detection of tetQ gene, which confers resistance to tetracycline, was included in the examination. The detection of P. gingivalis, P. intermedia and A. actinomycetemcomitans was linear over a range of 10-10(7) Cells (10-10(7) copies for tetQ gene), while the quantitative range for total bacteria was 10(2)-10(7) cells. Species-specific amplifications were observed for the three periodontal bacteria, and there was no significant difference between the TaqMan and SYBR Green chemistry in their specificity, quantitativity and sensitivity. The SYBR Green assay, which was simpler than TaqMan assay in its manipulations, was applied to the clinical plaque samples. The plaque samples were obtained from eight patients (eight periodontal pockets) before and 1 week after the local drug delivery of minocycline. Although the number of P. gingivalis, P. intermedia and A. actinomycetemcomitans markedly decreased after the antibiotic therapy in most cases, higher copy numbers of the tetQ gene were detectable. The real-time PCR demonstrated sufficient sensitivity, specificity and quantitativity to be a powerful tool for microbiological examination in periodontal disease, and the quantitative monitoring of antibiotic resistance gene accompanied with the antibiotic therapy should be included in the examination. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

    DOI: 10.1016/S0928-8244(03)00224-4

    Web of Science

    PubMed

    researchmap

  • Application of denaturing gradient gel electrophoresis (DGGE) to the analysis of microbial communities of subgingival plaque 査読 国際誌

    C Fujimoto, H Maeda, S Kokeguchi, S Takashiba, F Nishmura, H Arai, K Fukui, Y Murayama

    JOURNAL OF PERIODONTAL RESEARCH   38 ( 4 )   440 - 445   2003年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL MUNKSGAARD  

    Objectives: Denaturing gradient gel electrophoresis (DGGE) was applied to the microbiologic examination of subgingival plaque.
    Materials and methods: The PCR primers were designed from conserved nucleotide sequences on 16S ribosomal RNA gene (16SrDNA) with GC rich clamp at the 5'-end. Polymerase chain reaction (PCR) was performed using the primers and genomic DNAs of typical periodontal bacteria. The generated 16SrDNA fragments were separated by denaturing gel.
    Results: Although the sizes of the amplified DNA fragments were almost the same among the species, 16SrDNAs of the periodontal bacteria were distinguished according to their specific sequences. The microflora of clinical plaque samples were profiled by the PCR-DGGE method, and the dominant 16SrDNA bands were cloned and sequenced. Simultaneously, Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis and Prevotella intermedia were detected by ail ordinary PCR method. In the deep periodontal pockets, the bacterial community structures were complicated and P. gingivalis was the most dominant species, whereas the DGGE profiles were simple and Streptococcus or Neisseria species were dominant in the shallow pockets. The species-specific PCR method revealed the presence of A. actinomycetemcomitans, P. gingivalis and P. intermedia in the clinical samples. However, corresponding bands were not always observed in the DGGE profiles, indicating a lower sensitivity of the DGGE method.
    Conclusion: Although the DGGE method may have a lower sensitivity than the ordinary PCR methods, it could visualize the bacterial qualitative compositions and reveal the major species of the plaque. The DGGE analysis and following sequencing may have the potential to be a promising bacterial examination procedure in periodontal diseases.

    Web of Science

    PubMed

    researchmap

  • Gene profiling in human periodontal ligament fibroblasts by subtractive hybridization 査読 国際誌

    T Yamamoto, F Myokai, F Nishimura, T Ohira, N Shiomi, K Yamashiro, H Arai, Y Murayama, S Takashiba

    JOURNAL OF DENTAL RESEARCH   82 ( 8 )   641 - 645   2003年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R  

    Genes expressed by human periodontal ligament fibroblasts (HPFs)are likely to be associated with specific functions of the ligament. The aim of this study is to profile genes expressed highly by HPFs. A library (6 x 103 pfu)was constructed, followed by subtraction of HPF cDNAs with human gingival fibroblast (HGF) cDNAs. Reverse-dot hybridization revealed that 33 clones expressed higher levels of specific mRNAs in HPFs than in HGFs. These were mRNAs for known genes including several associated with maturation and differentiation of cells. None had been reported in PFs. One clone, PDL-29 identified as a COX assembly factor, showed much stronger mRNA expression in HPFs than in HGFs in culture. In rat periodontium, however, PDL-29 mRNA expression was similar in PFs and GFs. These results suggest that HPFs express many previously unreported genes associated with maturation and differentiation, but expression can differ in vitro and in vivo.

    Web of Science

    PubMed

    researchmap

  • Antimicrobial periodontal treatment decreases serum C-reactive protein, tumor necrosis factor-alpha, but not adiponectin levels in patients with chronic periodontitis 査読 国際誌

    Y Iwamoto, F Nishimura, Y Soga, K Takeuchi, M Kurihara, S Takashiba, Y Murayama

    JOURNAL OF PERIODONTOLOGY   74 ( 8 )   1231 - 1236   2003年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    Background: Elevated levels of C-reactive protein (CRP) and decreased plasma adiponectin are associated with increased risk of atherosclerosis. Furthermore, recent observations suggested that adiponectin and tumor necrosis factor-alpha (TNF-alpha) suppressed each other's production. Since periodontal disease has been suggested to act as a risk factor for atherosclerosis, we examined the effects of antimicrobial periodontal treatment on CRP, adiponectin, and TNF-a levels.
    Methods: Fifteen chronic periodontitis patients with various systemic conditions at high risk for atherosclerosis were enrolled in the study. Patients were non-surgically treated with topical application of antibiotics and mechanical debridement of calculus once a week for 1 month. Before and after therapy, CRP, adiponectin, and TNF-alpha levels were measured.
    Results: Both CRP and TNF-alpha levels were significantly decreased after treatment (P &lt;0.01 and P &lt;0.03, respectively), while adiponectin levels did not change significantly.
    Conclusions: Periodontal treatment is effective in reducing CRP and TNF-alpha, while adiponectin does not appear to be influenced by periodontal treatment. Elevated levels of CRP and TNF-alpha may be associated with increased risk for future development of atherosclerosis in periodontitis patients.

    DOI: 10.1902/jop.2003.74.8.1231

    Web of Science

    PubMed

    researchmap

  • Tumor necrosis factor-alpha gene (TNF-alpha)-1031/-863,-857 single-nucleotide polymorphisms (SNPs) are associated with severe adult periodontitis in Japanese 査読 国際誌

    Y Soga, F Nishimura, H Ohyama, H Maeda, S Takashiba, Y Murayama

    JOURNAL OF CLINICAL PERIODONTOLOGY   30 ( 6 )   524 - 531   2003年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL MUNKSGAARD  

    Objectives: Tumour necrosis factor-alpha (TNF-alpha ) and interleukin-1beta (IL-1beta ) participate in the establishment of inflammatory lesions in periodontitis. High production of these cytokines may relate to the severity of periodontitis. There have already been several studies examining the association between periodontitis and single nucleotide polymorphisms (SNPs) that affect cytokine productivity. Recently, new SNPs of TNF-alpha , -1031, -863 and -857, variants of which are observed in a relatively large proportion in Japanese, have been identified. The variant alleles of these SNPs have been suggested to be related to high TNF-alpha production. For a better understanding of the genetic factors associated with the severity of periodontitis, further analysis including these newly identified SNPs is essential. In addition, previous reports on TNF-alpha or IL-1beta SNPs associated with periodontitis were mainly for Caucasian populations. Therefore, the aim of this study is to examine the association between severe periodontitis in Japanese and the following SNPs: five in the TNF-alpha gene promoter (-1031, -863, -857, -308, -238) and three in the IL-1beta gene (-511, -31, +3953).
    Material and Methods: A total of 128 Japanese individuals were enrolled in this study. They were 64 patients with severe adult periodontitis and 64 healthy subjects. TNF-alpha and IL-1beta SNPs were genotyped by polymerase chain reaction-restriction fragment length polymorphism for all subjects. TNF-alpha and IL-1beta production from LPS-stimulated monocytes/macrophages was also measured for 15 healthy male subjects.
    Results: TNF-alpha production in TNF-alpha -1031/-863 (linkage disequilibrated) or -857 SNP variant allele carriers tended to be elevated, and the frequency of subjects who carried at least one variant allele in TNF-alpha -1031, -863 or -857 SNPs among severe periodontitis patients was significantly higher than in healthy subjects.
    Conclusion: Since the frequency of subjects who carried at least one variant allele in TNF-alpha -1031, -863 or -857 SNPs was higher in periodontitis patients than in healthy subjects, TNF-alpha -1031, -863 and -857 SNPs appear to be associated with severe adult periodontitis in Japanese populations.

    DOI: 10.1034/j.1600-051X.2003.00287.x

    Web of Science

    PubMed

    researchmap

  • Unique genes induced by mechanical stress in periodontal ligament cells 査読

    F Myokai, M Oyama, F Nishimura, T Ohira, T Yamamoto, H Arai, S Takashiba, Y Murayama

    JOURNAL OF PERIODONTAL RESEARCH   38 ( 3 )   255 - 261   2003年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL MUNKSGAARD  

    Objectives: The aim of this study is to isolate mechanical stress-induced genes (MSGens) from human periodontal ligament (PDL) cells and to analyze profiles of the mRNA expression of these genes.
    Background: Differential expression of genes in PDL cells under physiological stress such as occlusal force is thought to be orchestrated not only for the remodeling of PDL itself but also for the repair and regeneration of periodontal tissues. However, little is known about the genes expressed in PDL cells under mechanical stress.
    Methods: The cDNA from mechanical stress-applied human PDL cells was subtracted against the cDNA from static control cells. The subtracted cDNA was amplified by polymerase chain reaction (PCR) and cloned for further analysis.
    Results: Among 68 independent clones isolated, 15 contained DNA fragments greater than 250 bp. Reverse Northern analysis revealed a marked induction of MSGen-15 and MSGen-28 mRNA expression in the mechanical stress-applied cells. However, little difference in the magnitude of expression for the other MSGens was detected between the stress-applied cells and the control cells. After nucleotide sequencing and the analysis of homology with known genes, five clones were identified; ribosomal protein S27 (MSGen-9), MRG 15 (MSGen-15), androgen-binding protein (MSGen-18), cathepsin H (MSGen-28), and cytochrome c (MSGen-47). Interestingly, it has been reported that MRG 15 is a novel transcription factor involved in the regulation of cell growth and senescence. The remaining 10 clones, classified into six sequence types, had no significant homology with any known genes.
    Conclusions: These results suggest that many known and unknown genes are expressed in response to mechanical stress in PDL cells, and that a transcription factor, MRG 15, may be responsible for molecular events in PDL cells under mechanical stress.

    Web of Science

    researchmap

  • Ligation of IFN-gamma-induced HLA-DR molecules on fibroblasts induces RANTES expression via c-Jun N-terminal kinase (JNK) pathway 査読 国際誌

    M Meguro, F Nishimura, H Ohyama, S Takashiba, Y Murayama, S Matsushita

    CYTOKINE   22 ( 5 )   107 - 115   2003年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD  

    The role of human leukocyte antigen (HLA) class 11 Molecules on non-antigen presenting cells has been a matter of controversy. We recently reported that ligation of HLA-DR molecule with anti-HLA-DR antibodies (L243) and/or antigenic peptide/T cell receptor complex resulted in a secretion of several chemokines such as RANTES. In the present study, we aimed to detect putative signal transduction pathway leading to RANTES production from fibroblasts when the DR molecules were ligated with L243. Protein tyrosine kinase inhibitor (GF109203X) suppressed RANTES expression in a dose dependent manner for up to 50% from gingival fibroblasts (GF), while protein kinase C inhibitor (genistein) had no inhibitory effect. Ligation of DR molecules with L243 resulted in tyrosine phosphorylation of 54kDa cellular protein. Thus, we suspected that either Jun N-terminal kinase-2 (JNK-2) or Src family proteins were involved in HLA-DR-mediated signaling. JNK inhibitor (SP600125), but not Src inhibitor (PP2), suppressed both L243 stimulated RANTES mRNA expression and protein secretion. The maximum inhibition for RANTES production by SP600125 was more than 80%. Additionally, JNK inhibitor nearly completely blocked tumor necrosis factor-alpha (TNF-alpha)-induced RANTES production in GF. Furthermore, ligation of GF HLA-DR with L243 induced selective phosphorylation of JNK-2. We concluded that JNK-2 was one of the HLA-DR-mediated signal transduction pathways. (C) 2003 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/S1043-4666(03)00123-6

    Web of Science

    PubMed

    researchmap

  • Tumor necrosis factor-alpha gene (TNF-alpha)-1031/-863,-857 single-nucleotide polymorphisms (SNPs) are associated with severe adult periodontitis in Japanese 査読

    Y Soga, F Nishimura, H Ohyama, H Maeda, S Takashiba, Y Murayama

    JOURNAL OF CLINICAL PERIODONTOLOGY   30 ( 6 )   524 - 531   2003年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL MUNKSGAARD  

    Objectives: Tumour necrosis factor-alpha (TNF-alpha ) and interleukin-1beta (IL-1beta ) participate in the establishment of inflammatory lesions in periodontitis. High production of these cytokines may relate to the severity of periodontitis. There have already been several studies examining the association between periodontitis and single nucleotide polymorphisms (SNPs) that affect cytokine productivity. Recently, new SNPs of TNF-alpha , -1031, -863 and -857, variants of which are observed in a relatively large proportion in Japanese, have been identified. The variant alleles of these SNPs have been suggested to be related to high TNF-alpha production. For a better understanding of the genetic factors associated with the severity of periodontitis, further analysis including these newly identified SNPs is essential. In addition, previous reports on TNF-alpha or IL-1beta SNPs associated with periodontitis were mainly for Caucasian populations. Therefore, the aim of this study is to examine the association between severe periodontitis in Japanese and the following SNPs: five in the TNF-alpha gene promoter (-1031, -863, -857, -308, -238) and three in the IL-1beta gene (-511, -31, +3953).
    Material and Methods: A total of 128 Japanese individuals were enrolled in this study. They were 64 patients with severe adult periodontitis and 64 healthy subjects. TNF-alpha and IL-1beta SNPs were genotyped by polymerase chain reaction-restriction fragment length polymorphism for all subjects. TNF-alpha and IL-1beta production from LPS-stimulated monocytes/macrophages was also measured for 15 healthy male subjects.
    Results: TNF-alpha production in TNF-alpha -1031/-863 (linkage disequilibrated) or -857 SNP variant allele carriers tended to be elevated, and the frequency of subjects who carried at least one variant allele in TNF-alpha -1031, -863 or -857 SNPs among severe periodontitis patients was significantly higher than in healthy subjects.
    Conclusion: Since the frequency of subjects who carried at least one variant allele in TNF-alpha -1031, -863 or -857 SNPs was higher in periodontitis patients than in healthy subjects, TNF-alpha -1031, -863 and -857 SNPs appear to be associated with severe adult periodontitis in Japanese populations.

    DOI: 10.1034/j.1600-051X.2003.00287.x

    Web of Science

    researchmap

  • Unique genes induced by mechanical stress in periodontal ligament cells 査読 国際誌

    F Myokai, M Oyama, F Nishimura, T Ohira, T Yamamoto, H Arai, S Takashiba, Y Murayama

    JOURNAL OF PERIODONTAL RESEARCH   38 ( 3 )   255 - 261   2003年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL MUNKSGAARD  

    Objectives: The aim of this study is to isolate mechanical stress-induced genes (MSGens) from human periodontal ligament (PDL) cells and to analyze profiles of the mRNA expression of these genes.
    Background: Differential expression of genes in PDL cells under physiological stress such as occlusal force is thought to be orchestrated not only for the remodeling of PDL itself but also for the repair and regeneration of periodontal tissues. However, little is known about the genes expressed in PDL cells under mechanical stress.
    Methods: The cDNA from mechanical stress-applied human PDL cells was subtracted against the cDNA from static control cells. The subtracted cDNA was amplified by polymerase chain reaction (PCR) and cloned for further analysis.
    Results: Among 68 independent clones isolated, 15 contained DNA fragments greater than 250 bp. Reverse Northern analysis revealed a marked induction of MSGen-15 and MSGen-28 mRNA expression in the mechanical stress-applied cells. However, little difference in the magnitude of expression for the other MSGens was detected between the stress-applied cells and the control cells. After nucleotide sequencing and the analysis of homology with known genes, five clones were identified; ribosomal protein S27 (MSGen-9), MRG 15 (MSGen-15), androgen-binding protein (MSGen-18), cathepsin H (MSGen-28), and cytochrome c (MSGen-47). Interestingly, it has been reported that MRG 15 is a novel transcription factor involved in the regulation of cell growth and senescence. The remaining 10 clones, classified into six sequence types, had no significant homology with any known genes.
    Conclusions: These results suggest that many known and unknown genes are expressed in response to mechanical stress in PDL cells, and that a transcription factor, MRG 15, may be responsible for molecular events in PDL cells under mechanical stress.

    Web of Science

    PubMed

    researchmap

  • Antibacterial activity of synthetic human B defensin-2 against periodontal bacteria. 査読 国際誌

    Mineshiba F, Takashiba S, Mineshiba J, Matsuura K, Kokeguchi S, Murayama Y

    Journal of the International Academy of Periodontology   5 ( 2 )   35 - 40   2003年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:2  

    PubMed

    researchmap

  • Perspective of cytokine regulation for periodontal treatment: Fibroblast biology 査読 国際誌

    S Takashiba, K Naruishi, Y Murayama

    JOURNAL OF PERIODONTOLOGY   74 ( 1 )   103 - 110   2003年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    Efforts to understand the pathogenesis of periodontal diseases have been underway for decades. Studies of immunological aspects in addition to the structural components of gingival fibroblasts showed that the fibroblasts actively participate in immune and inflammatory events in periodontal diseases. Future strategies for the prevention and treatment of periodontal diseases should biologically regulate fibroblast activities. These cells are surrounded by monocyte-derived proinflammatory cytokines such as interleukin-1 beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha), and lymphocyte-derived interleukin-6 (IL-6) in inflamed gingival tissue. Recent anti-cytokine therapy for inflammatory diseases including rheumatoid arthritis aimed to inhibit the binding of cytokines to targeted cells such as fibroblasts and condrocytes. IL-1beta and TNF-alpha are thought to be therapeutic targets because these cytokines are essential for the initiation of inflammatory immune reactions and are produced for prolonged periods in inflammatory diseases. IL-6 is also a target, because it is abundantly present in inflammatory lesions and activates fibroblasts in the presence of soluble IL-6 receptor. In addition, these cytokines accelerate gingival fibroblasts to produce collagenolytic enzymes, resulting in tissue destruction. Soluble receptors for IL-1beta and TNF-alpha are suggested to be candidates for therapeutic molecules, but soluble receptor for IL-6 is suggested to be a factor-stimulating fibroblast. This paper will review the utilization of soluble receptors specific to inflammatory cytokines which potentially stimulate fibroblasts to regulate biological events involved in the pathogenesis of periodontal diseases.

    DOI: 10.1902/jop.2003.74.1.103

    Web of Science

    PubMed

    researchmap

  • Counter-antigen presentation: Fibroblasts produce cytokines by signalling through HLA class II molecules without inducing T-cell proliferation 査読 国際誌

    H Ohyama, F Nishimura, M Meguro, S Takashiba, Y Murayama, S Matsushita

    CYTOKINE   17 ( 4 )   175 - 181   2002年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD  

    Fibroblasts are known to express histocompatibility leukocyte antigen DR (HLA-DR) molecules on their cell surface upon stimulation with interferon gamma (IFN-gamma), while the exact roles of HLA-DR on fibroblasts remain undetermined. To understand the role of HLA-DR molecules on fibroblasts, we examined whether: (1) fibroblasts act as antigen presenting cells (APC) which activate helper T (Th) cells; and/or (2) fibroblasts are activated via HLA-II molecules by making a T-cell receptor (TCR)-peptide-major histocompatibility complex (MHC) complex. We used Th-0 clone HT8.3, which recognizes an antigenic peptide (Ag53 p141-161) in the context of DRB1*1501, as well as IFN-gamma-treated and irradiated periodontal ligament fibroblasts (PDL) expressing DRB1*1501 molecules. When peptide-pulsed fibroblasts were co-incubated with HT8.3 treated by the protein synthesis inhibitor emetine, peptide-induced de novo expression of lymphokines and cell-surface molecules on T cells can be neglected. The antigen presenting capacity of these fibroblasts was evaluated by examining the proliferative responses of Th cells. Possible activation of fibroblasts by stimulation via HLA-DR molecules was evaluated by quantitating secreted cytokines in the supernatants after 18-h culture with or without anti-HLA-DR monoclonal antibody (mAb) or emetine-treated HT8.3. Indeed, Th cells did not show proliferative responses when peptide-pulsed PDL were used as APC, whereas PDL produced larger amounts of interleukin (IL) 6, IL-8, monocyte chemoattractant protein 1 (MCP-1) and regulated upon activation, normal T expressed and secreted (RANTES) compared with controls, when cultured with anti-HLA-DR mAb or emetine-treated HT8.3. These findings suggest that HLA-DR expressed on fibroblasts do not present antigens to induce T-cell proliferation, but may act as receptor molecules that transmit signals into fibroblasts, based on DR-peptide-TCR interaction, resulting in the secretion of several cytokine species. (C) 2002 Elsvier Science Ltd. All rights reserved.

    DOI: 10.1006/cyto.2001.0976

    Web of Science

    PubMed

    researchmap

  • T cell responses to major membrane protein II (MMP II) of Mycobacterium leprae are restricted by HLA-DR molecules in patients with leprosy 査読 国際誌

    H Ohyama, S Matsushita, F Nishimura, N Kato, K Hatano, S Takashiba, Y Murayama

    VACCINE   20 ( 3-4 )   475 - 482   2001年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI LTD  

    Major membrane protein II (MMP II) of Mycobacterium leprae (M. leprae) is a 22 kDa protein inducing humoral immune response in leprosy patients. MMP II-specific bulk T cell lines were established from leprosy patients to determine major T cell epitopes in MMP Il and to evaluate lymphokine production induced by MMP II. These bulk T cell lines reacted to one or more peptides in the locus of amino acid residues from 23 to 109 of MMP II. The proliferative responses of all T cell lines were mainly inhibited by the addition of anti-DRB1 mAb. Many bulk T cell lines induced IFN-gamma. IL-5, but not IL-4. However, it was not possible to distinguish the LL or TT types of leprosy based on the pattern of T cell epitopes and the lymphokine productivity in the responses against MMP II, Thus, it appears that T cell response to MMP II is restricted by the HLA-DRB1 molecule, but not by DQ and DP molecules, which results in the induction of IFN-gamma production. (C) 2001 Elsevier Science Ltd. All rights reserved.

    DOI: 10.1016/S0264-410X(01)00354-1

    Web of Science

    PubMed

    researchmap

  • Subgingival microflora and antibody responses against periodontal bacteria of young Japanese patients with type 1 diabetes mellitus. 査読 国際誌

    Takahashi K, Nishimura F, Kurihara M, Iwamoto Y, Takashiba S, Miyata T, Murayama Y

    Journal of the International Academy of Periodontology   3 ( 4 )   104 - 111   2001年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:4  

    PubMed

    researchmap

  • Impairment of gingival fibroblast adherence by IL-6/sIL-6R 査読

    K Naruishi, S Takashiba, F Nishimura, HH Chou, H Arai, H Yamada, Y Murayama

    JOURNAL OF DENTAL RESEARCH   80 ( 5 )   1421 - 1424   2001年5月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC DENTAL RESEARCH  

    Interleukin-6 (IL-6) binds to human gingival fibroblasts (HGF) in the presence of a soluble form of IL-6 receptor (sIL-6R). We investigated the effects of IL-6 on the functions of HGF in the presence of sIL-6R. HGF changed their morphology from spindle-shaped to round, and detached from the culture dish by stimulation with IL-6/sIL-6R. In this condition, a signal transducer gp130 and a transcription factor Stat3 were phosphorylated, resulting in activation of transcription factors Stat3 and C/EBP beta. Cytoskeletal beta -actin and adhesion molecule integrin-alpha5, a subunit of alpha (5)beta (1) integrin (VLA-5), were found to possess potential binding domains for these transcription factors in their promoters. Accumulation of beta -actin and integrin-alpha5 mRNA decreased, contrary to the expectation of the induction of gene transcription. Furthermore, the decrease in their mRNAs was associated with reduced expression of both actin and VLA-5 proteins. These results suggest that the expression of VLA-5 and actin was down-regulated in HGF through an IL-6 signaling pathway, resulting in impairment of HGF adherence.

    Web of Science

    researchmap

  • Impairment of gingival fibroblast adherence by IL-6/sIL-6R 査読 国際誌

    K Naruishi, S Takashiba, F Nishimura, HH Chou, H Arai, H Yamada, Y Murayama

    JOURNAL OF DENTAL RESEARCH   80 ( 5 )   1421 - 1424   2001年5月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC DENTAL RESEARCH  

    Interleukin-6 (IL-6) binds to human gingival fibroblasts (HGF) in the presence of a soluble form of IL-6 receptor (sIL-6R). We investigated the effects of IL-6 on the functions of HGF in the presence of sIL-6R. HGF changed their morphology from spindle-shaped to round, and detached from the culture dish by stimulation with IL-6/sIL-6R. In this condition, a signal transducer gp130 and a transcription factor Stat3 were phosphorylated, resulting in activation of transcription factors Stat3 and C/EBP beta. Cytoskeletal beta -actin and adhesion molecule integrin-alpha5, a subunit of alpha (5)beta (1) integrin (VLA-5), were found to possess potential binding domains for these transcription factors in their promoters. Accumulation of beta -actin and integrin-alpha5 mRNA decreased, contrary to the expectation of the induction of gene transcription. Furthermore, the decrease in their mRNAs was associated with reduced expression of both actin and VLA-5 proteins. These results suggest that the expression of VLA-5 and actin was down-regulated in HGF through an IL-6 signaling pathway, resulting in impairment of HGF adherence.

    Web of Science

    PubMed

    researchmap

  • Identification and characterization of B-cell epitopes of a 53-kDa outer membrane protein from Porphyromonas gingivalis 査読 国際誌

    K Oyaizu, H Ohyama, F Nishimura, H Kurihara, S Matsushita, H Maeda, S Kokeguchi, H Hongyo, S Takashiba, Y Murayama

    ORAL MICROBIOLOGY AND IMMUNOLOGY   16 ( 2 )   73 - 78   2001年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MUNKSGAARD INT PUBL LTD  

    We have previously reported that Porphyromonas gingivalis FDC 381 possesses a 53-kDa protein antigen (Ag53) on its outer membrane that evokes a strong humoral immune response in many patients with periodontal disease and that the humoral immune responses to Ag53 differ greatly among patients. To understand how the individual humoral immune system against Ag53 was determined, the regions of Ag53 recognized by specific antibody (B-cell epitopes) and dominant subclasses of serum immunoglobulin G (IgG) against major B-cell epitopes were examined by enzyme-linked immunosorbent assay. This study used sera from six patients with periodontitis, which all reacted strongly with sonic extracts of P. gingivalis 381 and with purified Ag53, and sera from six periodontally healthy children, which did not react with either sonic extracts of P. gingivalis 381 or Ag53. The epitopes were identified using synthetic 5-residue overlapping decapeptides covering the entire Ag53. Thirteen of 89 synthetic decapeptides showed a strong reaction with sera from the periodontal patients, but no reaction with those from the healthy children. Four peptides of 13 exerted different immune responses among patients. Furthermore, restriction analyses of the highly antigenic regions revealed that three sequences, RAAIRAS, YYLQ and MSPARR, were identified as major B-cell epitopes. Additionally these epitopes were recognized mainly by the IgG2 isotype. These data suggest that the difference of B-cell epitopes might influence individual differences in antibody titer against Ag53 and also that the epitopes recognized commonly by multiple antibodies are quite valuable for peptide vaccine development against P. gingivalis infection.

    DOI: 10.1034/j.1399-302X.2001.016002073.x

    Web of Science

    PubMed

    researchmap

  • Serum phenytoin concentration and IgG antibody titre to periodontal bacteria in patients with phenytoin-induced gingival overgrowth. 査読 国際誌

    Yamada H, Nishimura F, Furuno K, Naruishi K, Kobayashi Y, Takashiba S, Murayama Y

    Journal of the International Academy of Periodontology   3 ( 2 )   42 - 47   2001年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:2  

    PubMed

    researchmap

  • Heterogeneity of host immunological risk factors in patients with aggressive periodontitis 査読 国際誌

    K Takahashi, H Ohyama, M Kitanaka, T Sawa, J Mineshiba, F Nishimura, H Arai, S Takashiba, Y Murayama

    JOURNAL OF PERIODONTOLOGY   72 ( 4 )   425 - 437   2001年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    Background: The pathogenesis of early-onset periodontitis (EOP) can be explained by various host risk factors. Previous studies have focused on a single (among many possible) immunological risk factor and the association among the factors has not been assessed. We comprehensively investigated the associations among multiple host immunological risk factors in EOP patients to further elucidate their role in the pathogenesis of EOP.
    Methods: Sixty-eight EOP patients (50 generalized EOP, 18 localized EOP), 51 EOP-suspected patients (S-EOP), 43 adult periodontitis (AP) patients, and 36 periodontally healthy subjects (HS) participated in this cross-sectional study. We examined peripheral neutrophil functions, phenotypic and functional characterization of peripheral lymphocytes (lymphocyte subsets, T-cell proliferative activity), cytokine productivity (interleukin [IL]-1, IL-2, tumor necrosis factor [TNF]-alpha, interferon [IFN]-gamma, IL-4 and IL-6), serum immunoglobulin G (IgG) antibody titers against 12 periodontal bacteria, and HLA class II genotypes.
    Results: G-EOP, S-EOP, and AP patient groups showed significantly lower percentages of pan T cells and CD8-positive cells (P&lt;0.02) compared with the HS group. L-EOP patients showed depressed IL-4 and TNF-&lt;alpha&gt; productivity compared with the HS group (P &lt;0.02). The EOP group showed significantly elevated antibody levels against Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola, and Fusobacterium nucleatum compared with the HS group (P &lt;0.05). The frequency with DQB1*0503 was significantly higher in the EOP patient group than the HS group (P = 0.045) due to the higher frequency in L-EOP patients than the HS group (P = 0.035). There were wide interindividual variations in each of the tests among patient and HS groups; however, EOP patients showed wider intradiagnostic group variations in certain host defensive cell functions than the other groups. There were some EOP patients who showed extremely low or high values in some tests; the EOP patients could be further divided into subgroups according to their host defensive and immunological profiles. However, there was heterogeneity in some of the other host immunological tests even in the subgroups.
    Conclusions: The association of host immunological risk factors in EOP patients is widely varied and more complex than previously thought. These results indicate the difficulty of explaining the pathogenesis of EOP based on a single host risk factor and also emphasize the importance of critical assessment of not only EOP patient groups, but also individual patients.

    DOI: 10.1902/jop.2001.72.4.425

    Web of Science

    PubMed

    researchmap

  • Immunopathological diagnosis of cicatricial pemphigoid with desquamative gingivitis. A case report 査読 国際誌

    M Kurihara, F Nishimura, T Hashimoto, A Komai, H Ueda, S Kokeguchi, S Takashiba, Y Murayama

    JOURNAL OF PERIODONTOLOGY   72 ( 2 )   243 - 249   2001年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    Cicatricial pemphigoid (CP) is a chronic subepidermal bullous dermatosis which primarily involves the mucous membranes. The oral cavity and the eye are most frequently involved. Since extension of the lesion into the pharynx and esophagus causes sore throat and dysphagia and progressive ocular lesions may cause blindness, early and valid diagnosis is very important. Here we present a case of cicatricial pemphigoid with onset at age 45 in a patient who manifested severe periodontal disease and showed the lesion on the mucous membranes of the mouth (desquamative gingivitis), skin, and eyes. Since definite diagnosis is very important, we describe how we made a differential diagnosis from other diseases which also accompany desquamative gingivitis. We examined the clinical manifestations, blood test results, HLA-genotype, histopathologic findings of the affected tissue, and immunological findings in relation to autoimmunity. Since many of the CP cases are first referred to periodontists or dentists, we believe that the diagnostic strategy described in the present study will be quite informative for making rapid and definite diagnoses of similar cases.

    DOI: 10.1902/jop.2001.72.2.243

    Web of Science

    PubMed

    researchmap

  • Characterization of two genes encoding ferritin-like protein in Actinobacillus actinomycetemcomitans 査読 国際誌

    M Hirosue, S Kokeguchi, H Maeda, F Nishimura, S Takashiba, Y Murayama

    MICROBIOLOGY AND IMMUNOLOGY   45 ( 10 )   721 - 727   2001年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CENTER ACADEMIC PUBL JAPAN  

    Two genes encoding ferritin-like protein, designated afnA and afnB, were identified in the upstream region of actX on the Actinobacillus actinomycetemcomitans chromosomal DNA. The actX has been reported to be a regulatory gene homologous to the Escherichia coli fnr, which controls the growth and virulence of A. actinomycetemcomitans under anaerobic conditions. The afnB located 340 bp-upstream from the actX, and the afnA located just 15 bp-upstream from afnB. The afnA and afnB encoded 161 and 165 amino acid residues, respectively, which were similar to ferritin-like proteins of other microorganisms. Western immunoblotting using rabbit antiserum against E. coli ferritin showed these two proteins, which are reactive with the serum with 19-kDa molecular masses, are produced from A. actinomycetemcomitans. The N-terminal amino acid sequences of the two proteins were consequent with those deduced from afnA and afnB. Northern hybridization revealed that the afnA and afnB constituted a bicistronic operon and the accumulation of afnA and afnB mRNA was upregulated under aerobic conditions. These findings suggested that the operon was regulated by the presence of oxygen. The two ferritin-like proteins may have important roles in the adaptation of A. actinomycetemcomitans to oxidative environmental changes.

    DOI: 10.1111/j.1348-0421.2001.tb01307.x

    Web of Science

    PubMed

    researchmap

  • Tumor necrosis factor-alpha (TNF-alpha)-induced and interleukin-1 beta (IL-1 beta)-induced shedding of TNF receptors from gingival fibroblasts 査読

    H Ohe, S Takashiba, K Naruishi, HH Chou, H Yamada, F Nishimura, H Arai, Y Murayama

    JOURNAL OF INTERFERON AND CYTOKINE RESEARCH   20 ( 12 )   1077 - 1082   2000年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MARY ANN LIEBERT INC PUBL  

    Tumor necrosis factor-alpha (TNF-alpha) exerts its functions by binding two different receptors (TNFR55 and TNFR75), Both TNFR55 and TNFR75 exist in cell-associated and soluble forms. Soluble TNF receptors (sTNFR), sTNFR55 and sTNFR75, are proteolytically shed upon inflammatory stimuli and then modulate various TNF-alpha bioactivities. As human gingival fibroblasts (HGF) can be potential targets for TNF-alpha in inflamed gingiva, we hypothesized that HGF partially modulate the cellular responses to TNF-alpha by regulating their own TNFR. In this study, the kinetics of expression of cell-associated and soluble forms of both receptors from cultured HGF in response to proinflammatory cytokines TNF-alpha and interleukin-1 beta (IL-1 beta) were investigated in vitro. Both TNF-alpha and IL-1 beta upregulated the gene expression of TNFR75 and did not affect that of TNFR55. TNF-alpha and IL-1 beta decreased binding of [I-125]TNF-alpha to HGF. Moreover, TNF-alpha and IL-1 beta upregulated the release of sTNFR75 from HGF but not that of sTNFR55, These results suggest that HGF under inflammatory conditions may contribute to the inactivation of circulating TNF-a through the preferential induction and shedding of TNFR75.

    DOI: 10.1089/107999000750053744

    Web of Science

    PubMed

    CiNii Article

    researchmap

  • Tumor necrosis factor-alpha (TNF-alpha)-induced and interleukin-1 beta (IL-1 beta)-induced shedding of TNF receptors from gingival fibroblasts 査読

    H Ohe, S Takashiba, K Naruishi, HH Chou, H Yamada, F Nishimura, H Arai, Y Murayama

    JOURNAL OF INTERFERON AND CYTOKINE RESEARCH   20 ( 12 )   1077 - 1082   2000年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MARY ANN LIEBERT INC PUBL  

    Tumor necrosis factor-alpha (TNF-alpha) exerts its functions by binding two different receptors (TNFR55 and TNFR75), Both TNFR55 and TNFR75 exist in cell-associated and soluble forms. Soluble TNF receptors (sTNFR), sTNFR55 and sTNFR75, are proteolytically shed upon inflammatory stimuli and then modulate various TNF-alpha bioactivities. As human gingival fibroblasts (HGF) can be potential targets for TNF-alpha in inflamed gingiva, we hypothesized that HGF partially modulate the cellular responses to TNF-alpha by regulating their own TNFR. In this study, the kinetics of expression of cell-associated and soluble forms of both receptors from cultured HGF in response to proinflammatory cytokines TNF-alpha and interleukin-1 beta (IL-1 beta) were investigated in vitro. Both TNF-alpha and IL-1 beta upregulated the gene expression of TNFR75 and did not affect that of TNFR55. TNF-alpha and IL-1 beta decreased binding of [I-125]TNF-alpha to HGF. Moreover, TNF-alpha and IL-1 beta upregulated the release of sTNFR75 from HGF but not that of sTNFR55, These results suggest that HGF under inflammatory conditions may contribute to the inactivation of circulating TNF-a through the preferential induction and shedding of TNFR75.

    Web of Science

    researchmap

  • Molecular characterization of the hlyX-like gene of Actinobacillus actinomycetemcomitans Y4 査読 国際誌

    S Kokeguchi, M Hirosue, H Maeda, M Miyamoto, S Takashiba, Y Murayama

    RESEARCH IN MICROBIOLOGY   151 ( 9 )   721 - 725   2000年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER  

    We isolated and characterized a possible regulatory gene, designated actX gene, from Actinobacillus actinomyctemcomitans Y4, which defined the Actinobacillus pleuropneumoniae hlyX-like regulatory gene. DNA sequence analysis for plasmid clone pKM317 containing a 1.6-kb DNA insert indicated an open reading frame encoding a polypeptide of 257 amino acid residues. Analysis of the deduced amino acid sequence showed the presence of five characteristic cysteine residues in the N-terminal region and a putative DNA binding residue in the C-terminal region, indicating that actX might belong to a regulatory gene family. Escherichia coli DH5 alpha and a mutant strain JRG1728 transformed by plasmid carrying actX manifested apparent hemolytic activity on sheep blood agar and grew anaerobically, although the original strains did not. (C) 2000 Editions scientifiques et medicales Elsevier SAS.

    DOI: 10.1016/S0923-2508(00)01137-2

    Web of Science

    PubMed

    researchmap

  • Molecular characterization of the hlyX-like gene of Actinobacillus actinomycetemcomitans Y4 査読

    S Kokeguchi, M Hirosue, H Maeda, M Miyamoto, S Takashiba, Y Murayama

    RESEARCH IN MICROBIOLOGY   151 ( 9 )   721 - 725   2000年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER  

    We isolated and characterized a possible regulatory gene, designated actX gene, from Actinobacillus actinomyctemcomitans Y4, which defined the Actinobacillus pleuropneumoniae hlyX-like regulatory gene. DNA sequence analysis for plasmid clone pKM317 containing a 1.6-kb DNA insert indicated an open reading frame encoding a polypeptide of 257 amino acid residues. Analysis of the deduced amino acid sequence showed the presence of five characteristic cysteine residues in the N-terminal region and a putative DNA binding residue in the C-terminal region, indicating that actX might belong to a regulatory gene family. Escherichia coli DH5 alpha and a mutant strain JRG1728 transformed by plasmid carrying actX manifested apparent hemolytic activity on sheep blood agar and grew anaerobically, although the original strains did not. (C) 2000 Editions scientifiques et medicales Elsevier SAS.

    DOI: 10.1016/S0923-2508(00)01137-2

    Web of Science

    researchmap

  • Antibody responses against Porphyromonas gingivalis infection in patients with early-onset periodontitis 査読 国際誌

    SJ Guo, K Takahashi, S Kokeguchi, S Takashiba, DF Kinane, Y Murayama

    JOURNAL OF CLINICAL PERIODONTOLOGY   27 ( 10 )   769 - 777   2000年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MUNKSGAARD INT PUBL LTD  

    Background, aims: The aim of this study was to evaluate antibody responses against Porphyromonas gingivalis (P. gingivalis) infection in early-onset periodontitis (EOP) patients to elucidate further the host-parasite interactions in the pathogenesis of EOP.
    Method: 16 P. gingivalis-infected EOP and 20 adult periodontitis (AP) patients, and 18 periodontally healthy subjects (HS) participated in this study. Serum immunoglobulin G (IgG) antibody levels and avidities against extracted P. gingivalis whole cells were measured. The components of P. gingivalis outer membrane antigens (OMA) reacting to patients' sera were analysed from the molecular weights by Western blotting. Serum antibody levels against P. gingivalis lipopolysaccharide (LPS) were also measured. The ability of the patients' sera to block interleukin-1 beta (IL-1 beta) production by human mononuclear cells in response to P. gingivalis LPS was examined.
    Results: Antibody levels were positively correlated with antibody avidities in both EOP and AP patients (r=0.91, r=0.72, p&lt;0.0005, respectively), while not significantly so in HS (r=0.09). There was variability in the antigen recognition of P. gingivalis OMA in EOP and AP patients. Smear and 53-kDa protein were more frequently recognized by sera of EOP and AP patients rather than that of HS (p&lt;0.05). The smear was partly diminished by absorption with P. gingivalis LPS, indicating the smear antigen was partly composed of LPS. There was high correlation between antibody levels against P. gingivalis whole-cell extracts and LPS in EOP and AP patients (r=0.81, p=0.0002, r=0.87, p&lt;0.0001, respectively), while not significant in HS (r=0.22). The sera of EOP and AP patients with high IgG titre to P. gingivalis LPS blocked IL-1 beta production more effectively than that of the patients with low IgG titre to P. gingivalis LPS.
    Conclusions: These results indicate that EOP patients' antibody response against P. gingivalis infection does not differ significantly from that of AP patients. The person-to-person heterogeneous antibody production against P. gingivalis LPS could contribute to our understanding of the relationship between the defensive ability of EOP patients and their chronic infection with this pathogen.

    Web of Science

    PubMed

    researchmap

  • Antibody responses against Porphyromonas gingivalis infection in patients with early-onset periodontitis 査読

    SJ Guo, K Takahashi, S Kokeguchi, S Takashiba, DF Kinane, Y Murayama

    JOURNAL OF CLINICAL PERIODONTOLOGY   27 ( 10 )   769 - 777   2000年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MUNKSGAARD INT PUBL LTD  

    Background, aims: The aim of this study was to evaluate antibody responses against Porphyromonas gingivalis (P. gingivalis) infection in early-onset periodontitis (EOP) patients to elucidate further the host-parasite interactions in the pathogenesis of EOP.
    Method: 16 P. gingivalis-infected EOP and 20 adult periodontitis (AP) patients, and 18 periodontally healthy subjects (HS) participated in this study. Serum immunoglobulin G (IgG) antibody levels and avidities against extracted P. gingivalis whole cells were measured. The components of P. gingivalis outer membrane antigens (OMA) reacting to patients' sera were analysed from the molecular weights by Western blotting. Serum antibody levels against P. gingivalis lipopolysaccharide (LPS) were also measured. The ability of the patients' sera to block interleukin-1 beta (IL-1 beta) production by human mononuclear cells in response to P. gingivalis LPS was examined.
    Results: Antibody levels were positively correlated with antibody avidities in both EOP and AP patients (r=0.91, r=0.72, p&lt;0.0005, respectively), while not significantly so in HS (r=0.09). There was variability in the antigen recognition of P. gingivalis OMA in EOP and AP patients. Smear and 53-kDa protein were more frequently recognized by sera of EOP and AP patients rather than that of HS (p&lt;0.05). The smear was partly diminished by absorption with P. gingivalis LPS, indicating the smear antigen was partly composed of LPS. There was high correlation between antibody levels against P. gingivalis whole-cell extracts and LPS in EOP and AP patients (r=0.81, p=0.0002, r=0.87, p&lt;0.0001, respectively), while not significant in HS (r=0.22). The sera of EOP and AP patients with high IgG titre to P. gingivalis LPS blocked IL-1 beta production more effectively than that of the patients with low IgG titre to P. gingivalis LPS.
    Conclusions: These results indicate that EOP patients' antibody response against P. gingivalis infection does not differ significantly from that of AP patients. The person-to-person heterogeneous antibody production against P. gingivalis LPS could contribute to our understanding of the relationship between the defensive ability of EOP patients and their chronic infection with this pathogen.

    Web of Science

    researchmap

  • Induction of intracellular interleukin-1 beta signals via type II interleukin-1 receptor in human gingival fibroblasts 査読 国際誌

    HH Chou, S Takashiba, H Maeda, K Naruishi, F Nishimura, H Arai, H Lu, Y Murayama

    JOURNAL OF DENTAL RESEARCH   79 ( 9 )   1683 - 1688   2000年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC DENTAL RESEARCH  

    The type II interleukin-1 receptor (IL-1RII) has been thought to be incapable of transducing signals to cells because of its short intracellular domain, while type I IL-1 receptor (IL-1RI) does transduce signals. Since over-expression of IL-1RII has been demonstrated to inhibit cytokine production in the fibroblastic cell line, it has been proposed to use IL-1RII to prevent IL-1-induced inflammation in connective tissue. In this study, trace amounts of IL-1RII mRNA expression were detected in human gingival fibroblasts (HGFs), which are affected by cytokines in inflammatory periodontal disease. Cloning of the cDNA encoding IL-1RII expressed in HGFs revealed 3 amino acid substitutions in the extracellular domain, when compared with the 408 residues predicted from human B-cells. Over-expression of IL-1RII on HGFs by gene transfer down-regulated the expression of IL-1 beta mRNA and IL-6 mRNA in response to IL-1 beta stimulation, while the expression of IL-8 mRNA was not affected. In the IL-1RII-transfected HGFs, phosphorylation of 25- and 74-kDa proteins was up-regulated upon IL-1 beta stimulation in the transfected HGFs. The phosphorylation of these proteins was suppressed by the addition of a neutralizing antibody against IL-1RII. These results suggest that the IL-1RII may regulate HGFs expression of cytokine mRNA upon IL-1 beta stimulation, possibly by altering the IL-1RI-dependent signals.

    Web of Science

    PubMed

    researchmap

  • Induction of intracellular interleukin-1 beta signals via type II interleukin-1 receptor in human gingival fibroblasts 査読

    HH Chou, S Takashiba, H Maeda, K Naruishi, F Nishimura, H Arai, H Lu, Y Murayama

    JOURNAL OF DENTAL RESEARCH   79 ( 9 )   1683 - 1688   2000年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC DENTAL RESEARCH  

    The type II interleukin-1 receptor (IL-1RII) has been thought to be incapable of transducing signals to cells because of its short intracellular domain, while type I IL-1 receptor (IL-1RI) does transduce signals. Since over-expression of IL-1RII has been demonstrated to inhibit cytokine production in the fibroblastic cell line, it has been proposed to use IL-1RII to prevent IL-1-induced inflammation in connective tissue. In this study, trace amounts of IL-1RII mRNA expression were detected in human gingival fibroblasts (HGFs), which are affected by cytokines in inflammatory periodontal disease. Cloning of the cDNA encoding IL-1RII expressed in HGFs revealed 3 amino acid substitutions in the extracellular domain, when compared with the 408 residues predicted from human B-cells. Over-expression of IL-1RII on HGFs by gene transfer down-regulated the expression of IL-1 beta mRNA and IL-6 mRNA in response to IL-1 beta stimulation, while the expression of IL-8 mRNA was not affected. In the IL-1RII-transfected HGFs, phosphorylation of 25- and 74-kDa proteins was up-regulated upon IL-1 beta stimulation in the transfected HGFs. The phosphorylation of these proteins was suppressed by the addition of a neutralizing antibody against IL-1RII. These results suggest that the IL-1RII may regulate HGFs expression of cytokine mRNA upon IL-1 beta stimulation, possibly by altering the IL-1RI-dependent signals.

    Web of Science

    researchmap

  • High glucose suppresses cathepsin activity in periodontal-ligament-derived fibroblastic cells 査読 国際誌

    F Nishimura, K Naruishi, H Yamada, T Kono, S Takashiba, Y Murayama

    JOURNAL OF DENTAL RESEARCH   79 ( 8 )   1614 - 1617   2000年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC DENTAL RESEARCH  

    The accumulation of extracellular matrices and integrins by high glucose has been reported in relation to diabetic complications. We previously reported that PDL cells expressed a higher amount of VLA-5 when cultured in high-glucose (4500 mg/L) medium than those cultured in low-glucose (1100 mg/L) medium. In this study, we aimed to address (1) whether this effect was mediated by the transcriptional level of the gene or the degradative level of the protein, and (2) whether this effect was mediated by TGF-beta. The results indicated that the level of mRNA encoding 015 integrin did not change in PDL cells regardless of the concentration of glucose. Alternatively, high glucose suppressed cathepsin B+L activity. Additionally, the level of mRNA encoding TGF-beta was not affected by high glucose, nor did an anti-TGF-beta neutralizing antibody have an effect on the expression of beta 5 gene or cathepsin activity. Therefore, the effects of high glucose appeared to be mediated by impaired protein degradation, but not by autocrine TGF-beta.

    Web of Science

    PubMed

    researchmap

  • High glucose suppresses cathepsin activity in periodontal-ligament-derived fibroblastic cells 査読

    F Nishimura, K Naruishi, H Yamada, T Kono, S Takashiba, Y Murayama

    JOURNAL OF DENTAL RESEARCH   79 ( 8 )   1614 - 1617   2000年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC DENTAL RESEARCH  

    The accumulation of extracellular matrices and integrins by high glucose has been reported in relation to diabetic complications. We previously reported that PDL cells expressed a higher amount of VLA-5 when cultured in high-glucose (4500 mg/L) medium than those cultured in low-glucose (1100 mg/L) medium. In this study, we aimed to address (1) whether this effect was mediated by the transcriptional level of the gene or the degradative level of the protein, and (2) whether this effect was mediated by TGF-beta. The results indicated that the level of mRNA encoding 015 integrin did not change in PDL cells regardless of the concentration of glucose. Alternatively, high glucose suppressed cathepsin B+L activity. Additionally, the level of mRNA encoding TGF-beta was not affected by high glucose, nor did an anti-TGF-beta neutralizing antibody have an effect on the expression of beta 5 gene or cathepsin activity. Therefore, the effects of high glucose appeared to be mediated by impaired protein degradation, but not by autocrine TGF-beta.

    Web of Science

    researchmap

  • Epitope mapping of heat shock protein 60 (GroEL) from Porphyromonas gingivalis 査読 国際誌

    H Maeda, M Miyamoto, S Kokeguchi, T Kono, F Nishimura, S Takashiba, Y Murayama

    FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY   28 ( 3 )   219 - 224   2000年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Porphyromonas gingivalis, a putative pathogen in human periodontal disease, possesses a 60-kDa heat shock protein (hsp60. GroEL). The GroEL homologs are known to be key molecules in auto-immune reactions because of the sequence similarity with human hsp60. In this study, B-cell epitopes on P. gingivalis GroEL (PgGroEL) were analyzed by both Western immunoblotting with truncated PgGroEL and by the multi-pin synthetic peptide approach. To examine auto-antibody production in periodontitis patients, Western immunoblotting with human gingival fibroblasts was performed. Deletion mutants were constructed from the cloned PgGroEL gene (P. gingivalis groEL), and four C-terminal truncated PgGroEL and one N-terminal truncated PgGroEL were prepared from the deletants. Sera from periodontitis patients reacted with all truncated PgGroEL used in this study. The results suggest that the B-cell epitopes were overlaid throughout PgGroEL. To determine the detailed locations of the B-cell epitope, 84 decapeptides covering the entire PgGroEL were synthesized and the serum IgG response to the peptides was examined. Epitope mapping using the synthetic peptides confirmed that the B-cell epitopes were overlaid throughout the length of PgGroEL and revealed that highly conserved peptides between PgGroEL and human hsp60 were recognized by the serum antibodies. Immuno-reactivity against human gingival fibroblasts was examined with sera from 30 periodontitis patients and 10 periodontally healthy subjects. IgG antibody against the 65-kDa antigen in human gingival fibroblasts (same molecular mass as human hsp60) was detected in two patients. Although IgG production against human hsp60 may be rare case in periodontitis patients, the results of epitope mapping demonstrated the potential of PgGroEL to cause the cross-reactions with human hsp60. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

    DOI: 10.1016/S0928-8244(00)00159-0

    Web of Science

    PubMed

    CiNii Article

    researchmap

  • Development of 16S rDNA-based PCR assay for detecting Centipeda periodontii and Selenomonas sputigena 査読

    S Sawada, S Kokeguchi, S Takashiba, Y Murayama

    LETTERS IN APPLIED MICROBIOLOGY   30 ( 6 )   423 - 426   2000年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL SCIENCE LTD  

    To detect oral motile bacteria directly from dental plaque, specific PCR primers for Centipeda periodontii and Selenomonas sputigena were designed based on the sequence analysis of their 16S rDNA. The primers were specific and sensitive enough to amplify DNA fragments from the available oral bacteria. The detection limit was fewer than 10 bacterial cells per sample. It was also possible to detect these bacteria in dental plaque. The prevalence of these bacteria varied in each sample. The specific primers designed in this study may clarify the epidemiology of periodontal disease.

    DOI: 10.1046/j.1472-765X.2000.00735.x

    Web of Science

    PubMed

    CiNii Article

    researchmap

  • Phenytoin and cyclosporin A suppress the expression of MMP-1, TIMP-1, and cathepsin L, but not cathepsin B in cultured gingival fibroblasts 査読 国際誌

    H Yamada, F Nishimura, K Naruishi, HH Chou, S Takashiba, GM Albright, S Nares, AM Iacopino, Y Murayama

    JOURNAL OF PERIODONTOLOGY   71 ( 6 )   955 - 960   2000年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    Background: Fibroblasts are known not only to synthesize and secrete extracellular matrix proteins, but also to degrade them for connective tissue remodeling. Drug-induced gingival overgrowth is characterized by a massive accumulation of extracellular matrix components in gingival connective tissues. Although some previous reports suggested that causative drugs stimulated the fibroblast proliferation, the results are not conclusive yet. In this study, we hypothesized that drug-induced gingival overgrowth could be a consequence of impaired ability of matrix degradation rather than an enhanced proliferation of gingival fibroblasts induced by these drugs.
    Methods: Normal human gingival fibroblasts were cultured with or without either 20 mug/ml of phenytoin or 200 ng/ml of cyclosporin A. Total RNA and cellular proteins were collected every day for RT-PCR analyses and for measuring lysosomal enzyme activity. In addition, an immunohistochemical study was performed to detect lysosomal enzymes in cells from enlarged gingiva of the patients with phenytoin-induced gingival overgrowth.
    Results: RT-PCR analyses revealed that these drugs suppressed the expression of MMP-1, TIMP-1, and cathepsin L, but not that of cathepsin B in a time-dependent manner. Then, we measured the activity of lysosomal enzymes and cathepsin B and L. The results indicated that although cathepsin B activity was not observed to be impaired, regardless of the drugs used in these cells, both total and active forms of combined activity of cathepsins B and L were suppressed in a time-dependent manner.
    Conclusions: The results indicate that, besides suggested effects of these drugs on gingival fibroblasts and/or on accumulated cells in the gingival tissues, extracellular matrix-degrading ability, particularly that by cathepsin L, is also suppressed by cyclosporin A and phenytoin in gingival fibroblasts, and that lysosomal enzyme plays an important role in the pathogenesis of drug-induced gingival hyperplasia.

    DOI: 10.1902/jop.2000.71.6.955

    Web of Science

    PubMed

    researchmap

  • Phenytoin and cyclosporin A suppress the expression of MMP-1, TIMP-1, and cathepsin L, but not cathepsin B in cultured gingival fibroblasts 査読

    H Yamada, F Nishimura, K Naruishi, HH Chou, S Takashiba, GM Albright, S Nares, AM Iacopino, Y Murayama

    JOURNAL OF PERIODONTOLOGY   71 ( 6 )   955 - 960   2000年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    Background: Fibroblasts are known not only to synthesize and secrete extracellular matrix proteins, but also to degrade them for connective tissue remodeling. Drug-induced gingival overgrowth is characterized by a massive accumulation of extracellular matrix components in gingival connective tissues. Although some previous reports suggested that causative drugs stimulated the fibroblast proliferation, the results are not conclusive yet. In this study, we hypothesized that drug-induced gingival overgrowth could be a consequence of impaired ability of matrix degradation rather than an enhanced proliferation of gingival fibroblasts induced by these drugs.
    Methods: Normal human gingival fibroblasts were cultured with or without either 20 mug/ml of phenytoin or 200 ng/ml of cyclosporin A. Total RNA and cellular proteins were collected every day for RT-PCR analyses and for measuring lysosomal enzyme activity. In addition, an immunohistochemical study was performed to detect lysosomal enzymes in cells from enlarged gingiva of the patients with phenytoin-induced gingival overgrowth.
    Results: RT-PCR analyses revealed that these drugs suppressed the expression of MMP-1, TIMP-1, and cathepsin L, but not that of cathepsin B in a time-dependent manner. Then, we measured the activity of lysosomal enzymes and cathepsin B and L. The results indicated that although cathepsin B activity was not observed to be impaired, regardless of the drugs used in these cells, both total and active forms of combined activity of cathepsins B and L were suppressed in a time-dependent manner.
    Conclusions: The results indicate that, besides suggested effects of these drugs on gingival fibroblasts and/or on accumulated cells in the gingival tissues, extracellular matrix-degrading ability, particularly that by cathepsin L, is also suppressed by cyclosporin A and phenytoin in gingival fibroblasts, and that lysosomal enzyme plays an important role in the pathogenesis of drug-induced gingival hyperplasia.

    Web of Science

    researchmap

  • Cell surface-associated enolase in Actinobacillus actinomycetemcomitans 査読 国際誌

    H Hara, H Ohta, T Inoue, T Ohashi, S Takashiba, Y Murayama, K Fukui

    MICROBIOLOGY AND IMMUNOLOGY   44 ( 5 )   349 - 356   2000年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CENTER ACADEMIC PUBL JAPAN  

    Cell surface-associated materials of Actinobacillus actinomycetemcomitans were extracted by a short incubation of the cell suspension in a Tris-buffered saline in the presence and absence of a restriction enzyme, EcoRI, The supernatants (which we termed EcoRI extract and surface extract, respectively) contained a number of extracellularly released proteins. Of these proteins, four major proteins were identified by N-terminal sequencing to be the 34 and 39 kDa outer membrane proteins, the GroEL-like protein, and a 47 kDa protein homologous to Haemophilus influenzae enolase, Enolase activity was found in the extracts and its relative amount of activity in the EcoRI extract from a culture of the mid-exponential growth phase was estimated as 5.7% of total enzyme activity, In contrast, the relative amount of activity of another cytosolic enzyme, lactate dehydrogenase, was extremely low in the extracts and also in the culture supernatant, These results suggest the external localization of enolase in this bacterium.

    Web of Science

    PubMed

    researchmap

  • Identification by subtractive hybridization of a novel insertion sequence specific for virulent strains of Porphyromonas gingivalis 査読 国際誌

    K Sawada, S Kokeguchi, H Hongyo, S Sawada, M Miyamoto, H Maeda, F Nishimura, S Takashiba, Y Murayama

    INFECTION AND IMMUNITY   67 ( 11 )   5621 - 5625   1999年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    Subtractive hybridization was employed to isolate specific genes from virulent Porphyromonas gingivalis strains that are possibly related to abscess formation. The genomic DNA from the virulent strain P, gingivalis W83 was subtracted with DNA from the avirulent strain ATCC 33277, Three clones unique to strain W83 were isolated and sequenced. The cloned DNA fragments were 885, 369, and 132 bp and had slight homology with only Bacillus stearothermophilus IS5377, which is a putative transposase, The regions flanking the cloned DNA fragments were isolated and sequenced, and the gene structure around the clones was revealed. These three clones were located side-by-side in a gene reported as an outer membrane protein. The three clones interrupt the open reading frame of the outer membrane protein gene. This inserted DNA, consisting of three isolated clones, was designated IS1598, which was 1,396 bp (i.e., a 1,158-bp open reading frame) in length and was flanked by 16-bp terminal inverted repeats and a 9-bp duplicated target sequence. IS1598 was detected in P. gingivalis W83, W50, and FDC 381 by Southern hybridization. All three P. gingivalis strains have been shown to possess abscess-forming ability in animal models. However, IS1598 was not detected in avirulent strains of P,gingivalis, including ATCC 33277, The IS1598 may interrupt the synthesis of the outer membrane protein, resulting in changes in the structure of the bacterial outer membrane. The IS1598 isolated in this study is a novel insertion element which might be a specific marker for virulent P. gingivalis strains.

    Web of Science

    PubMed

    researchmap

  • Identification by subtractive hybridization of a novel insertion sequence specific for virulent strains of Porphyromonas gingivalis 査読

    K Sawada, S Kokeguchi, H Hongyo, S Sawada, M Miyamoto, H Maeda, F Nishimura, S Takashiba, Y Murayama

    INFECTION AND IMMUNITY   67 ( 11 )   5621 - 5625   1999年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    Subtractive hybridization was employed to isolate specific genes from virulent Porphyromonas gingivalis strains that are possibly related to abscess formation. The genomic DNA from the virulent strain P, gingivalis W83 was subtracted with DNA from the avirulent strain ATCC 33277, Three clones unique to strain W83 were isolated and sequenced. The cloned DNA fragments were 885, 369, and 132 bp and had slight homology with only Bacillus stearothermophilus IS5377, which is a putative transposase, The regions flanking the cloned DNA fragments were isolated and sequenced, and the gene structure around the clones was revealed. These three clones were located side-by-side in a gene reported as an outer membrane protein. The three clones interrupt the open reading frame of the outer membrane protein gene. This inserted DNA, consisting of three isolated clones, was designated IS1598, which was 1,396 bp (i.e., a 1,158-bp open reading frame) in length and was flanked by 16-bp terminal inverted repeats and a 9-bp duplicated target sequence. IS1598 was detected in P. gingivalis W83, W50, and FDC 381 by Southern hybridization. All three P. gingivalis strains have been shown to possess abscess-forming ability in animal models. However, IS1598 was not detected in avirulent strains of P,gingivalis, including ATCC 33277, The IS1598 may interrupt the synthesis of the outer membrane protein, resulting in changes in the structure of the bacterial outer membrane. The IS1598 isolated in this study is a novel insertion element which might be a specific marker for virulent P. gingivalis strains.

    Web of Science

    researchmap

  • Differentiation of monocytes to macrophages primes cells for lipopolysaccharide stimulation via accumulation of cytoplasmic nuclear factor kappa B 査読

    S Takashiba, TE Van Dyke, S Amar, Y Murayama, AW Soskolne, L Shapira

    INFECTION AND IMMUNITY   67 ( 11 )   5573 - 5578   1999年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    During infection, circulating blood monocytes migrate from the vasculature to the extravascular compartments where they mature into tissue macrophages. The maturation process prepares the cell to actively participate in the inflammatory and the immune responses, and many transcription factors have been found to be involved. Here we report on a novel role for nuclear factor kappa B (NF-kappa B) in this process. Its accumulation in the cytoplasm of differentiated macrophages is responsible for the enhanced ability of the cell to respond to lipopolysaccharide (LPS) stimulation, as determined by tumor necrosis factor alpha (TNF-alpha) secretion. Differentiation of the human monocytic cell line THP-1 into macrophage-like cells was induced by exposure of the cells to phorbol myristate acetate. DNA-bindable NF-kappa B was not detected in the cytoplasm of undifferentiated THP-1 cells but accumulated in the cytoplasm of the cells following differentiation. No TNF-alpha was detected in the media of resting differentiated and nondifferentiated THP-1 cells. Stimulation with LPS of differentiated cells induced the production of higher levels of TNF-alpha than stimulation of nondifferentiated cells. This hyperresponsiveness to LPS was found in the mRNA and secreted TNF-alpha levels. Furthermore, stimulation with LPS induced the translocation of NF-kappa B from the cytoplasm into the nucleus. This translocation process was more rapid in the differentiated cells than in the nondifferentiated cells, and the resultant accumulated levels of NF-kappa B in the nucleus were higher. The DNA-bindable NF-kappa B was identified as a heterodimer of p65 and p50. The results suggest that NF-kappa B accumulation in the cytoplasm during maturation of monocytes to macrophages primes the cells for enhanced responsiveness to LPS and results in the rapid secretion of inflammatory mediators, such as TNF-alpha, by mature macrophages following LPS challenge.

    Web of Science

    researchmap

  • Differentiation of monocytes to macrophages primes cells for lipopolysaccharide stimulation via accumulation of cytoplasmic nuclear factor kappa B 査読 国際誌

    S Takashiba, TE Van Dyke, S Amar, Y Murayama, AW Soskolne, L Shapira

    INFECTION AND IMMUNITY   67 ( 11 )   5573 - 5578   1999年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    During infection, circulating blood monocytes migrate from the vasculature to the extravascular compartments where they mature into tissue macrophages. The maturation process prepares the cell to actively participate in the inflammatory and the immune responses, and many transcription factors have been found to be involved. Here we report on a novel role for nuclear factor kappa B (NF-kappa B) in this process. Its accumulation in the cytoplasm of differentiated macrophages is responsible for the enhanced ability of the cell to respond to lipopolysaccharide (LPS) stimulation, as determined by tumor necrosis factor alpha (TNF-alpha) secretion. Differentiation of the human monocytic cell line THP-1 into macrophage-like cells was induced by exposure of the cells to phorbol myristate acetate. DNA-bindable NF-kappa B was not detected in the cytoplasm of undifferentiated THP-1 cells but accumulated in the cytoplasm of the cells following differentiation. No TNF-alpha was detected in the media of resting differentiated and nondifferentiated THP-1 cells. Stimulation with LPS of differentiated cells induced the production of higher levels of TNF-alpha than stimulation of nondifferentiated cells. This hyperresponsiveness to LPS was found in the mRNA and secreted TNF-alpha levels. Furthermore, stimulation with LPS induced the translocation of NF-kappa B from the cytoplasm into the nucleus. This translocation process was more rapid in the differentiated cells than in the nondifferentiated cells, and the resultant accumulated levels of NF-kappa B in the nucleus were higher. The DNA-bindable NF-kappa B was identified as a heterodimer of p65 and p50. The results suggest that NF-kappa B accumulation in the cytoplasm during maturation of monocytes to macrophages primes the cells for enhanced responsiveness to LPS and results in the rapid secretion of inflammatory mediators, such as TNF-alpha, by mature macrophages following LPS challenge.

    Web of Science

    PubMed

    researchmap

  • HLA genetics for diagnosis of susceptibility to early-onset periodontitis 査読 国際誌

    S Takashiba, H Ohyama, K Oyaizu, N Kogoe-Kato, Y Murayama

    JOURNAL OF PERIODONTAL RESEARCH   34 ( 7 )   374 - 378   1999年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MUNKSGAARD INT PUBL LTD  

    Human leukocyte antigens (HLA) are essential in the recognition of foreign antigens in humoral immune response, which is genetically predetermined. Susceptibility to certain diseases that involve the immune response has been studied in relation to distinct HLA types. Although some diseases have been found Co correlate to specific HLA loci positively, it has been difficult to isolate HLA types that predispose patients to periodontal destruction. Here. we review the current knowledge and recent advances in HLA genetics and its biology, which determine susceptibility to early-onset periodontitis (EOP). The HLA-DRB1*1501-DQB1*0602 genotype has been found with increasing frequency in EOP patients. This HLA genotype expresses aspartic acid at position 57 and glycine at position 70 on the DQ beta chain, suggesting a capability to bind certain bacterial antigens. The T cell response against the outer membrane protein (Ag53) of Porphyromonas against gingivalis was examined via this HLA genotype. Strong T cell response against Ag53 p141-161 was inhibited partially by anti-DR antibody, but not by anti-DQ antibody, possible host and bacterial peptides capable of binding DRB1*1501 were elucidated when the peptide sequence was compared to gene and protein databases. These results suggest that patients who have the HLA-DRB1*1501-DQB1*0602 genotype may have an accelerated T cell response to certain periodontapathic bacteria such as P. gingivalis in hyperimmune reactions and thus increased susceptibility to EOP.

    Web of Science

    PubMed

    researchmap

  • HLA genetics for diagnosis of susceptibility to early-onset periodontitis 査読

    S Takashiba, H Ohyama, K Oyaizu, N Kogoe-Kato, Y Murayama

    JOURNAL OF PERIODONTAL RESEARCH   34 ( 7 )   374 - 378   1999年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MUNKSGAARD INT PUBL LTD  

    Human leukocyte antigens (HLA) are essential in the recognition of foreign antigens in humoral immune response, which is genetically predetermined. Susceptibility to certain diseases that involve the immune response has been studied in relation to distinct HLA types. Although some diseases have been found Co correlate to specific HLA loci positively, it has been difficult to isolate HLA types that predispose patients to periodontal destruction. Here. we review the current knowledge and recent advances in HLA genetics and its biology, which determine susceptibility to early-onset periodontitis (EOP). The HLA-DRB1*1501-DQB1*0602 genotype has been found with increasing frequency in EOP patients. This HLA genotype expresses aspartic acid at position 57 and glycine at position 70 on the DQ beta chain, suggesting a capability to bind certain bacterial antigens. The T cell response against the outer membrane protein (Ag53) of Porphyromonas against gingivalis was examined via this HLA genotype. Strong T cell response against Ag53 p141-161 was inhibited partially by anti-DR antibody, but not by anti-DQ antibody, possible host and bacterial peptides capable of binding DRB1*1501 were elucidated when the peptide sequence was compared to gene and protein databases. These results suggest that patients who have the HLA-DRB1*1501-DQB1*0602 genotype may have an accelerated T cell response to certain periodontapathic bacteria such as P. gingivalis in hyperimmune reactions and thus increased susceptibility to EOP.

    Web of Science

    researchmap

  • Chemotactic response of periodontal ligament cells decreases with donor age: association with reduced expression of c-fos 査読

    Y Asahara, F Nishimura, H Arai, H Kurihara, S Takashiba, Y Murayama

    ORAL DISEASES   5 ( 4 )   337 - 343   1999年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:STOCKTON PRESS  

    OBJECTIVE: To understand the effects of aging on cellular motility of human periodontal ligament (PDL) cells, and to determine the possible link between cell proliferation and migration in relation to cellular aging.
    MATERIALS AND METHODS: The chemotactic response of PDL cells from three juvenile and four older donors were compared. Then, migrated or unmigrated cells were examined for their cell cycle by morphological and immunocytochemical procedures, Finally, migrated or unmigrated cells were examined for the expression of c-fos and c-myc by in situ hybridization.
    RESULTS: PDL cells from older donors showed lower chemotaxis compared with the cells from juvenile donors (P &lt; 0.05), Cells undergoing migration were found not to be in the S- or M-phase of the cell cycle. However, all migrated cells were found to express c-fos, while many of the cells which did not migrate were found not to express c-fos,
    CONCLUSIONS: Cellular motility of PDL cells decreases with donor age as well as cell proliferation. Since the cells reaching senescence fail to express c-fos, the mechanisms linked to cellular senescence may be a possible underlying mechanism for low migration seen in the older cells.

    Web of Science

    researchmap

  • Chemotactic response of periodontal ligament cells decreases with donor age: association with reduced expression of c-fos 査読 国際誌

    Y Asahara, F Nishimura, H Arai, H Kurihara, S Takashiba, Y Murayama

    ORAL DISEASES   5 ( 4 )   337 - 343   1999年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:STOCKTON PRESS  

    OBJECTIVE: To understand the effects of aging on cellular motility of human periodontal ligament (PDL) cells, and to determine the possible link between cell proliferation and migration in relation to cellular aging.
    MATERIALS AND METHODS: The chemotactic response of PDL cells from three juvenile and four older donors were compared. Then, migrated or unmigrated cells were examined for their cell cycle by morphological and immunocytochemical procedures, Finally, migrated or unmigrated cells were examined for the expression of c-fos and c-myc by in situ hybridization.
    RESULTS: PDL cells from older donors showed lower chemotaxis compared with the cells from juvenile donors (P &lt; 0.05), Cells undergoing migration were found not to be in the S- or M-phase of the cell cycle. However, all migrated cells were found to express c-fos, while many of the cells which did not migrate were found not to express c-fos,
    CONCLUSIONS: Cellular motility of PDL cells decreases with donor age as well as cell proliferation. Since the cells reaching senescence fail to express c-fos, the mechanisms linked to cellular senescence may be a possible underlying mechanism for low migration seen in the older cells.

    Web of Science

    PubMed

    researchmap

  • Role of soluble interleukin-6 receptor in inflamed gingiva for binding of interleukin-6 to gingival fibroblasts 査読

    K Naruishi, S Takashiba, HH Chou, H Arai, F Nishimura, Y Murayama

    JOURNAL OF PERIODONTAL RESEARCH   34 ( 6 )   296 - 300   1999年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MUNKSGAARD INT PUBL LTD  

    Interleukin-6 (IL-6), frequently detected in periodontitis, is known to mediate important signals in the inflammatory cytokine network. Gingival fibroblasts (GF) secrete cytokines upon stimulation with inflammatory mediators. However, it is not clear if GF respond to IL-6. We examined the lL-6 receptor gene expression in GF. Furthermore, we tested whether GF are target cells for IL-6 by examination of binding of IL-6. GF were found to contain trace amounts of mRNA for IL-6 receptor (IL-GR), but had high levels of mRNA for 130-kDa glycoprotein (gp130), which is a signal transducer for IL-6/IL-6R complex. Based on this observation, we hypothesized that IL-6 could bind GF if exogenous soluble forms of IL-6R (sIL-6R) existed in the gingiva or culture condition. Thus, we investigated the existence of sIL-6R in gingiva using enzyme-linked immunosorbent assay and whether sIL-6R influenced the binding of IL-6 to GF in vitro. In inflamed gingiva, sIL-6R was detected and its concentration ranged from 150 to 700 pg/mu g protein. The sIL-6R enhanced the binding of IL-6 to GF in a dose-dependent manner. This enhancement was inhibited by an antibody against gp130, suggesting that the IL-6/sIL-6R complex bound to the fibroblasts via gp130. These data demonstrated that gingival fibroblasts can be target cells for IL-6 in the presence of appropriate amounts of sIL-6R. This situation may exist during inflammation in periodontal tissue.

    Web of Science

    researchmap

  • Role of soluble interleukin-6 receptor in inflamed gingiva for binding of interleukin-6 to gingival fibroblasts 査読 国際誌

    K Naruishi, S Takashiba, HH Chou, H Arai, F Nishimura, Y Murayama

    JOURNAL OF PERIODONTAL RESEARCH   34 ( 6 )   296 - 300   1999年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MUNKSGAARD INT PUBL LTD  

    Interleukin-6 (IL-6), frequently detected in periodontitis, is known to mediate important signals in the inflammatory cytokine network. Gingival fibroblasts (GF) secrete cytokines upon stimulation with inflammatory mediators. However, it is not clear if GF respond to IL-6. We examined the lL-6 receptor gene expression in GF. Furthermore, we tested whether GF are target cells for IL-6 by examination of binding of IL-6. GF were found to contain trace amounts of mRNA for IL-6 receptor (IL-GR), but had high levels of mRNA for 130-kDa glycoprotein (gp130), which is a signal transducer for IL-6/IL-6R complex. Based on this observation, we hypothesized that IL-6 could bind GF if exogenous soluble forms of IL-6R (sIL-6R) existed in the gingiva or culture condition. Thus, we investigated the existence of sIL-6R in gingiva using enzyme-linked immunosorbent assay and whether sIL-6R influenced the binding of IL-6 to GF in vitro. In inflamed gingiva, sIL-6R was detected and its concentration ranged from 150 to 700 pg/mu g protein. The sIL-6R enhanced the binding of IL-6 to GF in a dose-dependent manner. This enhancement was inhibited by an antibody against gp130, suggesting that the IL-6/sIL-6R complex bound to the fibroblasts via gp130. These data demonstrated that gingival fibroblasts can be target cells for IL-6 in the presence of appropriate amounts of sIL-6R. This situation may exist during inflammation in periodontal tissue.

    Web of Science

    PubMed

    researchmap

  • A novel lipopolysaccharide-induced transcription factor regulating tumor necrosis factor alpha gene expression: Molecular cloning, sequencing, characterization, and chromosomal assignment 査読 国際誌

    F Myokai, S Takashiba, R Lebo, S Amar

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   96 ( 8 )   4518 - 4523   1999年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATL ACAD SCIENCES  

    Lipopolysaccharide (LPS) is a potent stimulator of monocytes and macrophages, causing secretion of tumor necrosis factor alpha (TNF-alpha) and other inflammatory mediators. Given the deleterious effects to the host of TNF-alpha, it has been postulated that TNF-alpha gene expression must be tightly regulated. The nature of the nuclear factor(s) that control TNF-alpha gene transcription in humans remains obscure, although NF-kappa B has been suggested. Our previous studies pertaining to macrophage response to LPS identified a novel DNA-binding domain located from -550 to -487 in the human TNF-alpha promoter that contains transcriptional activity, but lacks any known NF-kappa B-binding sites. We have used this DNA fragment to isolate and purify a 60-kDa protein binding to this fragment and obtained its aminoterminal sequence, which was used to design degenerate probes to screen a cDNA library from THP-1 cells. A novel cDNA done (1.8 kb) was isolated and fully sequenced. Characterization of this cDNA clone revealed that its induction was dependent on LPS activation of THP-1 cells; hence, the name LPS-induced TNF-alpha factor (LITAF). Inhibition of LITAF mRNA expression in THP-1 cells resulted in a reduction of TNF-alpha transcripts. In addition, high level of expression of LITAF mRNA was observed predominantly in the placenta, peripheral blood leukocytes, lymph nodes, and the spleen. Finally, chromosomal localization using fluorescence in situ hybridization revealed that LITAF mapped to chromosome 16p12-16p13.3. Together, these findings suggest that LITAF plays an important role in the activation of the human TNF-alpha gene and proposes a new mechanism to control TNF-alpha gene expression.

    DOI: 10.1073/pnas.96.8.4518

    Web of Science

    PubMed

    CiNii Article

    researchmap

  • A novel lipopolysaccharide-induced transcription factor regulating tumor necrosis factor alpha gene expression: Molecular cloning, sequencing, characterization, and chromosomal assignment 査読

    F Myokai, S Takashiba, R Lebo, S Amar

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   96 ( 8 )   4518 - 4523   1999年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATL ACAD SCIENCES  

    Lipopolysaccharide (LPS) is a potent stimulator of monocytes and macrophages, causing secretion of tumor necrosis factor alpha (TNF-alpha) and other inflammatory mediators. Given the deleterious effects to the host of TNF-alpha, it has been postulated that TNF-alpha gene expression must be tightly regulated. The nature of the nuclear factor(s) that control TNF-alpha gene transcription in humans remains obscure, although NF-kappa B has been suggested. Our previous studies pertaining to macrophage response to LPS identified a novel DNA-binding domain located from -550 to -487 in the human TNF-alpha promoter that contains transcriptional activity, but lacks any known NF-kappa B-binding sites. We have used this DNA fragment to isolate and purify a 60-kDa protein binding to this fragment and obtained its aminoterminal sequence, which was used to design degenerate probes to screen a cDNA library from THP-1 cells. A novel cDNA done (1.8 kb) was isolated and fully sequenced. Characterization of this cDNA clone revealed that its induction was dependent on LPS activation of THP-1 cells; hence, the name LPS-induced TNF-alpha factor (LITAF). Inhibition of LITAF mRNA expression in THP-1 cells resulted in a reduction of TNF-alpha transcripts. In addition, high level of expression of LITAF mRNA was observed predominantly in the placenta, peripheral blood leukocytes, lymph nodes, and the spleen. Finally, chromosomal localization using fluorescence in situ hybridization revealed that LITAF mapped to chromosome 16p12-16p13.3. Together, these findings suggest that LITAF plays an important role in the activation of the human TNF-alpha gene and proposes a new mechanism to control TNF-alpha gene expression.

    DOI: 10.1073/pnas.96.8.4518

    Web of Science

    researchmap

  • In vitro induction of activation-induced cell death in lymphocytes from chronic periodontal lesions by exogenous Fas ligand 査読 国際誌

    T Sawa, F Nishimura, H Ohyama, K Takahashi, S Takashiba, Y Murayama

    INFECTION AND IMMUNITY   67 ( 3 )   1450 - 1454   1999年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    Periodontitis is a chronic inflammatory disease which gradually destroys the supporting tissues of the teeth, leading to tooth loss in adults, The lesions are characterized by a persistence of inflammatory cells in gingival and periodontal connective tissues. To understand what mechanisms are involved in the establishment of chronic lesions, we hypothesized that infiltrating lymphocytes might be resistant to apoptosis. However, both Bcl-2 and Bcl-xL were weakly detected in lymphocytes from the lesions, compared with those from peripheral blood, suggesting that these cells are susceptible to apoptosis, Nevertheless, very few apoptotic cells were observed in tissue sections from the lesions. Lymphocytes from the lesions expressed mRNA encoding Fas, whereas Fas-ligand mRNA was very weakly expressed in lymphocytes from the lesions and in periodontal tissues. Since the results indicated that lymphocytes in the lesions might be susceptible to Fas-mediated apoptosis but lack the death signal, we next investigated if these lymphocytes actually undergo apoptosis by the addition of anti-Fas antibodies in vitro. Fas-positive lymphocytes from the lesions underwent apoptosis by these antibodies, but Fas-negative lymphocytes and Fas-positive peripheral lymphocytes did not undergo apoptosis by these antibodies. These results indicate that lymphocytes in the lesions are susceptible to activation-induced cell death and are induced to die by apoptosis after the addition of exogenous Fas ligand.

    Web of Science

    PubMed

    researchmap

  • In vitro induction of activation-induced cell death in lymphocytes from chronic periodontal lesions by exogenous Fas ligand 査読

    T Sawa, F Nishimura, H Ohyama, K Takahashi, S Takashiba, Y Murayama

    INFECTION AND IMMUNITY   67 ( 3 )   1450 - 1454   1999年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    Periodontitis is a chronic inflammatory disease which gradually destroys the supporting tissues of the teeth, leading to tooth loss in adults, The lesions are characterized by a persistence of inflammatory cells in gingival and periodontal connective tissues. To understand what mechanisms are involved in the establishment of chronic lesions, we hypothesized that infiltrating lymphocytes might be resistant to apoptosis. However, both Bcl-2 and Bcl-xL were weakly detected in lymphocytes from the lesions, compared with those from peripheral blood, suggesting that these cells are susceptible to apoptosis, Nevertheless, very few apoptotic cells were observed in tissue sections from the lesions. Lymphocytes from the lesions expressed mRNA encoding Fas, whereas Fas-ligand mRNA was very weakly expressed in lymphocytes from the lesions and in periodontal tissues. Since the results indicated that lymphocytes in the lesions might be susceptible to Fas-mediated apoptosis but lack the death signal, we next investigated if these lymphocytes actually undergo apoptosis by the addition of anti-Fas antibodies in vitro. Fas-positive lymphocytes from the lesions underwent apoptosis by these antibodies, but Fas-negative lymphocytes and Fas-positive peripheral lymphocytes did not undergo apoptosis by these antibodies. These results indicate that lymphocytes in the lesions are susceptible to activation-induced cell death and are induced to die by apoptosis after the addition of exogenous Fas ligand.

    Web of Science

    researchmap

  • Phylogenetic characterization of Centipeda periodontii, Selenomonas sputigena and Selenomonas species by 16S rRNA gene sequence analysis 査読 国際誌

    S Sawada, S Kokeguchi, F Nishimura, S Takashiba, Y Murayama

    MICROBIOS   98 ( 391 )   133 - 140   1999年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:FACULTY PRESS  

    The nearly complete 16S rRNA gene sequences for oral Gram-negative anaerobic motile bacteria, Centipeda periodontii, Selenomonas sputigena and Selenomonas species (formerly S. sputigena type strain), were determined in order to unveil their relationship to other oral motile bacteria. To determine the phylogenetic characterization of these bacteria, their 16S rRNA gene sequences were obtained and compared with those from the ribosomal sequence databases previously reported. The 16S rRNA gene sequences of these bacteria were similar to those of Selenomonas ruminantium and Schwartzia succinivorans isolated from rumens, and to Pectinatus cerevisiiphilus isolated from spoiled beer. Among oral bacteria, the nucleotide sequence analysis of these bacteria revealed high nucleotide similarity to Veillonella species, whereas low similarity to oral motile bacteria such as Campylobacter species. Phylogenetic analysis clearly confirmed that C. periodontii and two Selenomonas species were classified as relatives of a group besides Selenomonas, Schwartzia, and Pectinatus species, and not as close relatives to oral motile bacteria, such as Campylobacter species. These results suggest that such oral Gram-negative anaerobic motile bacteria are close relatives of oral bacteria.

    Web of Science

    PubMed

    researchmap

  • T cell responses to 53-kDa outer membrane protein of Porphyromonas gingivalis in humans with early-onset periodontitis 査読

    H Ohyama, S Matsushita, N Kato, F Nishimura, K Oyaizu, S Kokeguchi, H Kurihara, S Takashiba, Y Nishimura, Y Murayama

    HUMAN IMMUNOLOGY   59 ( 10 )   635 - 643   1998年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    Patients with early-onset periodontitis (EOP) are susceptible to infection with periodontopathic bacteria, such as Porphyromonas gingivalis. Ag53, 53-kDa outer membrane protein of P. gingivalis, evokes strong humoral immune responses in EOP patients. In a first step to clarify how host immune cells recognize Ag53, we established Ag53-specific short-term T cell lines from 22 subjects including 6 EOP patients and 16 healthy donors, using overlapping peptides based on Ag53 amino acid sequences. All T cell lines from active EOP patients recognized a common region (p141-181, especially p141-161) on Ag53, while those from healthy donors showed heterogeneous specificity. p141-181 was not recognized by T cell lines established from EOP patients following therapy. A monoclonal antibody to HSA-DRB1 inhibited Ag53-induced proliferation of most of the T cell lines. Our observations suggest that, although antigen-presenting molecules are common in EOP patients and in healthy individuals, p141-161 includes a major T cell epitope(s) on Ag53 for active EOP patients but not for healthy individuals or inactive EOP patients. (C) American Society for Histocompatibility and Immunogenetics, 1998 Published by Elsevier Science Inc.

    DOI: 10.1016/S0198-8859(98)00064-0

    Web of Science

    researchmap

  • T cell responses to 53-kDa outer membrane protein of Porphyromonas gingivalis in humans with early-onset periodontitis 査読 国際誌

    H Ohyama, S Matsushita, N Kato, F Nishimura, K Oyaizu, S Kokeguchi, H Kurihara, S Takashiba, Y Nishimura, Y Murayama

    HUMAN IMMUNOLOGY   59 ( 10 )   635 - 643   1998年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    Patients with early-onset periodontitis (EOP) are susceptible to infection with periodontopathic bacteria, such as Porphyromonas gingivalis. Ag53, 53-kDa outer membrane protein of P. gingivalis, evokes strong humoral immune responses in EOP patients. In a first step to clarify how host immune cells recognize Ag53, we established Ag53-specific short-term T cell lines from 22 subjects including 6 EOP patients and 16 healthy donors, using overlapping peptides based on Ag53 amino acid sequences. All T cell lines from active EOP patients recognized a common region (p141-181, especially p141-161) on Ag53, while those from healthy donors showed heterogeneous specificity. p141-181 was not recognized by T cell lines established from EOP patients following therapy. A monoclonal antibody to HSA-DRB1 inhibited Ag53-induced proliferation of most of the T cell lines. Our observations suggest that, although antigen-presenting molecules are common in EOP patients and in healthy individuals, p141-161 includes a major T cell epitope(s) on Ag53 for active EOP patients but not for healthy individuals or inactive EOP patients. (C) American Society for Histocompatibility and Immunogenetics, 1998 Published by Elsevier Science Inc.

    DOI: 10.1016/S0198-8859(98)00064-0

    Web of Science

    PubMed

    CiNii Article

    researchmap

  • The S-layer protein from Campylobacter rectus: sequence determination and function of the recombinant protein 査読 国際誌

    M Miyamoto, H Maeda, M Kitanaka, S Kokeguchi, S Takashiba, Y Murayama

    FEMS MICROBIOLOGY LETTERS   166 ( 2 )   275 - 281   1998年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    The gene encoding the crystalline surface layer (S-layer) protein from Campylobacter rectus, designated sip, was sequenced and the recombinant gene product was expressed in Escherichia coli. The gene consisted of 4086 nucleotides encoding a protein with 1361 amino acids. The N-terminal amino acid sequence revealed that Sip did not contain a signal sequence, but that the initial methionine residue was processed. The deduced amino acid sequence displayed some common characteristic features of S-layer proteins previously reported. A homology search showed a high similarity to the Campylobacter fetus S-layer proteins, especially in their N-terminus. The C-terminal third of Sip exhibited homology with the RTX toxins from Gram-negative bacteria via the region including the glycine-rich repeats. The Sip protein had the same N-terminal sequence as a 104-kDa cytotoxin isolated from the culture supernatants of C. rectus. However, neither native nor recombinant Sip showed cytotoxicity against HL-60 cells or human peripheral white blood cells. These data support the idea that the N-terminus acts as an anchor to the cell surface components and that the C-terminus is involved in the assembly and/or transport of the protein. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

    DOI: 10.1016/S0378-1097(98)00343-7

    Web of Science

    PubMed

    CiNii Article

    researchmap

  • The S-layer protein from Campylobacter rectus: sequence determination and function of the recombinant protein 査読

    M Miyamoto, H Maeda, M Kitanaka, S Kokeguchi, S Takashiba, Y Murayama

    FEMS MICROBIOLOGY LETTERS   166 ( 2 )   275 - 281   1998年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    The gene encoding the crystalline surface layer (S-layer) protein from Campylobacter rectus, designated sip, was sequenced and the recombinant gene product was expressed in Escherichia coli. The gene consisted of 4086 nucleotides encoding a protein with 1361 amino acids. The N-terminal amino acid sequence revealed that Sip did not contain a signal sequence, but that the initial methionine residue was processed. The deduced amino acid sequence displayed some common characteristic features of S-layer proteins previously reported. A homology search showed a high similarity to the Campylobacter fetus S-layer proteins, especially in their N-terminus. The C-terminal third of Sip exhibited homology with the RTX toxins from Gram-negative bacteria via the region including the glycine-rich repeats. The Sip protein had the same N-terminal sequence as a 104-kDa cytotoxin isolated from the culture supernatants of C. rectus. However, neither native nor recombinant Sip showed cytotoxicity against HL-60 cells or human peripheral white blood cells. These data support the idea that the N-terminus acts as an anchor to the cell surface components and that the C-terminus is involved in the assembly and/or transport of the protein. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

    DOI: 10.1016/S0378-1097(98)00343-7

    Web of Science

    researchmap

  • Periodontal disease as a complication of diabetes mellitus. 査読 国際誌

    Nishimura F, Takahashi K, Kurihara M, Takashiba S, Murayama Y

    Annals of periodontology / the American Academy of Periodontology   3 ( 1 )   20 - 29   1998年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:1  

    PubMed

    researchmap

  • Identification of a novel insertion sequence element from Porphyromonas gingivalis W83 査読

    Sawada K, Kokeguchi S, Hongyo H, Sawada S, Hirosue M, Fujimoto C, Wataki T, Shimizu A, Katayama T, Kawabata E, Miyamoto M, Takashiba S, Murayama Y

    JOURNAL OF DENTAL RESEARCH   77 ( 5 )   1299   1998年5月

  • The inhibition of DNA synthesis by prostaglandin E-2 in human gingival fibroblasts is independent of the cyclic AMP-protein kinase A signal transduction pathway 査読 国際誌

    H Arai, Y Nomura, M Kinoshita, F Nishimura, M Takigawa, K Takahashi, N Washio, S Takashiba, Y Murayama

    JOURNAL OF PERIODONTAL RESEARCH   33 ( 1 )   33 - 39   1998年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL MUNKSGAARD  

    In this study we attempted to clarify the mechanism of the inhibitory effects of PGE(2) on DNA synthesis in Gin-1 (fibroblasts derived from healthy human gingiva) from the aspect of the cyclic AMP-dependent protein kinase signal transduction pathway. PGE(2) upregulated intracellular cyclic AMP accumulation and inhibited DNA synthesis in Gin-1 in a dose-dependent manner. When the PGE(2)-induced intracellular cyclic AMP accumulation was further enhanced by treatment with the cyclic AMP-phosphodiesterase inhibitor, IBMX, the inhibitory effect of PGE(2) on DNA synthesis was also enhanced. Furthermore, when we examined the effects of forskolin, an activator of cyclic AMP production, on intracellular cyclic AMP accumulation and DNA synthesis, similar results were obtained. However, inhibitors of cyclic AMP-dependent protein kinase (protein kinase A) such as HA1004 did not diminish the inhibitory effect of PGE(2) on DNA synthesis in Gin-1. These results suggest that in Gin-1, PGE(2)-induced cyclic AMP accumulation may not lead to the activation of protein kinase A or protein kinase A activity may not relate directly to the growth inhibitory effect of PGE(2), and that PGE(2) does not inhibit DNA synthesis through the cyclic AMP-protein kinase A signal transduction pathway in Gin-1.

    Web of Science

    PubMed

    researchmap

  • The inhibition of DNA synthesis by prostaglandin E-2 in human gingival fibroblasts is independent of the cyclic AMP-protein kinase A signal transduction pathway 査読

    H Arai, Y Nomura, M Kinoshita, F Nishimura, M Takigawa, K Takahashi, N Washio, S Takashiba, Y Murayama

    JOURNAL OF PERIODONTAL RESEARCH   33 ( 1 )   33 - 39   1998年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL MUNKSGAARD  

    In this study we attempted to clarify the mechanism of the inhibitory effects of PGE(2) on DNA synthesis in Gin-1 (fibroblasts derived from healthy human gingiva) from the aspect of the cyclic AMP-dependent protein kinase signal transduction pathway. PGE(2) upregulated intracellular cyclic AMP accumulation and inhibited DNA synthesis in Gin-1 in a dose-dependent manner. When the PGE(2)-induced intracellular cyclic AMP accumulation was further enhanced by treatment with the cyclic AMP-phosphodiesterase inhibitor, IBMX, the inhibitory effect of PGE(2) on DNA synthesis was also enhanced. Furthermore, when we examined the effects of forskolin, an activator of cyclic AMP production, on intracellular cyclic AMP accumulation and DNA synthesis, similar results were obtained. However, inhibitors of cyclic AMP-dependent protein kinase (protein kinase A) such as HA1004 did not diminish the inhibitory effect of PGE(2) on DNA synthesis in Gin-1. These results suggest that in Gin-1, PGE(2)-induced cyclic AMP accumulation may not lead to the activation of protein kinase A or protein kinase A activity may not relate directly to the growth inhibitory effect of PGE(2), and that PGE(2) does not inhibit DNA synthesis through the cyclic AMP-protein kinase A signal transduction pathway in Gin-1.

    Web of Science

    researchmap

  • Comparative study of two outer membrane protein genes from Porphyromonas gingivalis 査読 国際誌

    H Hongyo, S Kokeguchi, H Kurihara, M Miyamoto, H Maeda, S Takashiba, Y Murayama

    MICROBIOS   95 ( 381 )   91 - 100   1998年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:FACULTY PRESS  

    A periodontal pathogen, Porphyromonas gingivalis possesses either a 53 kD (Ag53) or a 67 kD (Ag67) outer membrane protein (OMP). Almost all sera from patients with periodontal diseases reacted strongly with either Ag53 or Ag67. In previous work the cloning and sequencing of the 53 kD outer membrane protein gene designated pga53 from Fl gingivalis FDC381, was reported and the presence of a gene homologous to pga53 in Fl gingivalis ATCC 33277 demonstrated. In the present work this pga53-homologous gene from Fl gingivalis ATCC 33277 was isolated and characterized. Nucleotide sequence analysis revealed that this gene encoded Ag67, and the gene was designated pga67. The deduced amino acid sequence and composition of pga67 was similar to the amino acid composition and N-terminal partial sequence of Ag67. An open reading frame of pga67 consisted of 1,692 nucleotides encoded as 564 amino acids, including a 49 amino acid signal sequence. The comparative analysis between pga67 and pga53 revealed that (1) the deduced amino acid sequence showed a 30.1% homology; (2) signal sequence and proline-rich regions at the C-terminus were the most conserved regions; (3) considerable differences were found mainly in the middle part of the OMPs; and (4) obvious differences in the two-dimensional models were evoked. These differences between pga67 and pga53 may explain the antigenic diversity between Ag67 and Ag53 OMPs.

    Web of Science

    PubMed

    researchmap

  • Association with Porphyromonas gingivalis infection in early-onset periodontitis patients. 査読

    Guo SJ, Takahashi K, Kokeguchi S, Wataki T, Fujimoto C, Katayama T, Ogawa E, Arai H, Nishimura F, Takashiba S, Murayama Y

    JOURNAL OF DENTAL RESEARCH   77   919   1998年

  • Characteristics of outer membrane protein of Actinobacillus actinomycetemcomitans grown with maltose. 査読

    Kokeguchi S, Miyamoto M, Maeda H, Hongyo H, Hirosue M, Sawada K, Takashiba S, Murayama Y

    JOURNAL OF DENTAL RESEARCH   77   985   1998年

  • Host defensive, immunological, and microbiological observations of an early-onset periodontitis patient with virus-associated hemophagocytic syndrome 査読

    T Kono, M Takigawa, F Nishimura, S Takashiba, M Nakagawa, H Maeda, H Arai, A Nagai, H Kurihara, Y Murayama

    JOURNAL OF PERIODONTOLOGY   68 ( 12 )   1223 - 1230   1997年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    VIRUS-ASSOCIATED HEMOPHAGOCYTIC SYNDROME (VAHS) is a disorder characterized by benign generalized histiocytic proliferation and marked hemophagocytosis associated with systemic viral infection. An immunodeficiency which includes an extremely decreased leukocyte and platelet count together with abnormalities in the CD4/CD8 ratio are the most common features of VAHS. Here we report an early-onset periodontitis (EOP) patient with VAHS from the standpoint of host-parasite interaction to understand the effect of this systemic disorder which might possibly influence susceptibility to periodontal disease. The patient is a 16-year-old Japanese male clinically diagnosed as having generalized EOP with slight gingival inflammation and moderate bone loss. This patient manifested VAHS at 3 years of age, and then had an unusual 4 recurrences (at 5, 7, 11, and 14 years old). Laboratory tests conducted include: 1) complete blood analyses; 2) peripheral neutrophil functions (chemotaxis, phagocytosis, superoxide production, and adherence); 3) peripheral lymphocyte subpopulations and functions, T-cell proliferative activity and productivity of cytokines (interleukin-2 [IL-2], interferon gamma [IFN-gamma], and tumor necrosis factor alpha [TNF-alpha]); 4) serum cytokine levels cn. Ip, IL-2, soluble IL-2 receptor [sIL-2R], IL-4, IL-6, IFN-gamma, and TNF-alpha; 5) serum immunoglobulin G (IgG) antibody titers against periodontopathic bacteria; 6) serological human leukocyte antigen (HLA) typing; and 7) determination of bacterial flora of the periodontal pockets. The results indicated that the patient's neutrophil chemotaxis and random migration were below the normal range. In lymphocyte examinations, T-cell proliferative activity, IL-2, and IFN-gamma productivity were elevated. Serum IFN-gamma level was also significantly higher than normal range. No specific periodontopathic bacteria were predominant in the periodontal pockets, however, the serum IgG titer against Porphyromonas gingivalis was elevated throughout the examination period. It is suggested that VAHS might be a possible risk factor for periodontal disease, and hence may serve as a model in understanding the role of host defense mechanisms in the establishment of inflammatory periodontal disease.

    Web of Science

    PubMed

    researchmap

  • Host defensive, immunological, and microbiological observations of an early-onset periodontitis patient with virus-associated hemophagocytic syndrome 査読

    T Kono, M Takigawa, F Nishimura, S Takashiba, M Nakagawa, H Maeda, H Arai, A Nagai, H Kurihara, Y Murayama

    JOURNAL OF PERIODONTOLOGY   68 ( 12 )   1223 - 1230   1997年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    VIRUS-ASSOCIATED HEMOPHAGOCYTIC SYNDROME (VAHS) is a disorder characterized by benign generalized histiocytic proliferation and marked hemophagocytosis associated with systemic viral infection. An immunodeficiency which includes an extremely decreased leukocyte and platelet count together with abnormalities in the CD4/CD8 ratio are the most common features of VAHS. Here we report an early-onset periodontitis (EOP) patient with VAHS from the standpoint of host-parasite interaction to understand the effect of this systemic disorder which might possibly influence susceptibility to periodontal disease. The patient is a 16-year-old Japanese male clinically diagnosed as having generalized EOP with slight gingival inflammation and moderate bone loss. This patient manifested VAHS at 3 years of age, and then had an unusual 4 recurrences (at 5, 7, 11, and 14 years old). Laboratory tests conducted include: 1) complete blood analyses; 2) peripheral neutrophil functions (chemotaxis, phagocytosis, superoxide production, and adherence); 3) peripheral lymphocyte subpopulations and functions, T-cell proliferative activity and productivity of cytokines (interleukin-2 [IL-2], interferon gamma [IFN-gamma], and tumor necrosis factor alpha [TNF-alpha]); 4) serum cytokine levels cn. Ip, IL-2, soluble IL-2 receptor [sIL-2R], IL-4, IL-6, IFN-gamma, and TNF-alpha; 5) serum immunoglobulin G (IgG) antibody titers against periodontopathic bacteria; 6) serological human leukocyte antigen (HLA) typing; and 7) determination of bacterial flora of the periodontal pockets. The results indicated that the patient's neutrophil chemotaxis and random migration were below the normal range. In lymphocyte examinations, T-cell proliferative activity, IL-2, and IFN-gamma productivity were elevated. Serum IFN-gamma level was also significantly higher than normal range. No specific periodontopathic bacteria were predominant in the periodontal pockets, however, the serum IgG titer against Porphyromonas gingivalis was elevated throughout the examination period. It is suggested that VAHS might be a possible risk factor for periodontal disease, and hence may serve as a model in understanding the role of host defense mechanisms in the establishment of inflammatory periodontal disease.

    Web of Science

    researchmap

  • Comparison of in vitro proliferative capacity of human periodontal ligament cells in juvenile and aged donors 査読 国際誌

    F. Nishimura, V. P. Terranova, M. Braithwaite, R. Orman, H. Ohyama, J. Mineshiba, H. H. Chou, S. Takashiba, Y. Murayama

    Oral Diseases   3 ( 3 )   162 - 166   1997年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:3  

    OBJECTIVE: The aim of this study is to compare the in vitro proliferative capacity of periodontal ligament (PDL) cells from aged and juvenile donors. MATERIALS AND METHODS: Flow-cytometric analysis of the cell cycle was used to compare the length of each cell cycle, and the ratio of the cells progressing through the cycles between four PDL cells from juvenile donors and four cells from aged donors. Then, replicative capacity of the PDL cells from three juvenile and three aged donors was compared by serial cultures. Finally, expression of c-fos was compared between cells proliferating and cells which had reached senescent. RESULTS: Flow- cytometric analysis of the cell cycle had revealed that although there were no differences in the length of each phase of the cell cycle, significant differences were found in the ratio of the cells entering from Gap I to DNA synthesis phase of the cell cycle (P &lt
    0.025). Replicative capacity was much longer in two cells from juvenile donors (about 20 population doublings), while all cells from aged donors showed short dividing abilities (less than eight population doublings), hence entered senescent phases shortly. Additionally, no c-fos was detected in cells which had reached senescence upon stimulation with serum. CONCLUSIONS: It is generally believed that aged humans have an impaired wound healing ability. We believe that more fibrotic PDL tissues seen in aged humans might be the reason for this, and suggest that this phenomena might be due to the progressive accumulation of senescent cell populations.

    Scopus

    PubMed

    researchmap

  • The regulatory effect of fermentable sugar levels on the production of leukotoxin by Actinobacillus actinomycetemcomitans 査読 国際誌

    K Mizoguchi, H Ohta, A Miyagi, H Kurihara, S Takashiba, K Kato, Y Murayama, K Fukui

    FEMS MICROBIOLOGY LETTERS   146 ( 1 )   161 - 166   1997年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    The relationship between sugar availability and RTX (repeats in toxin) cytotoxin (leukotoxin) production in the periodontopathic bacterium, Actinobacillus actinomycetemcomitans, was investigated using a chemostat. A. actinomycetemcomitans 301-b produced significant amounts of leukotoxin in anaerobic fructose-limited chemostat cultures at a dilution rate of 0.15 h(-1) and at pH 7.0. When the growth limitation was relieved by pulsing the cultures with 50 or 150 mM fructose (final concentrations), leukotoxin production immediately stopped and the amount of cellular leukotoxin decreased until the culture was returned to fructose-limited conditions. Leukotoxin synthesis was also repressed in the chemostat cultures by pulsing with glucose but not with the non-fermentable sugar analog, alpha-methyl-D-glucoside. Leukotoxin production was also repressed by fructose in chemostat cultures of ATCC 33384, which is generally recognized as a non-leukotoxin-producing or minimally leukotoxic strain.

    DOI: 10.1016/S0378-1097(96)00473-9

    Web of Science

    PubMed

    CiNii Article

    researchmap

  • The regulatory effect of fermentable sugar levels on the production of leukotoxin by Actinobacillus actinomycetemcomitans 査読

    K Mizoguchi, H Ohta, A Miyagi, H Kurihara, S Takashiba, K Kato, Y Murayama, K Fukui

    FEMS MICROBIOLOGY LETTERS   146 ( 1 )   161 - 166   1997年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    The relationship between sugar availability and RTX (repeats in toxin) cytotoxin (leukotoxin) production in the periodontopathic bacterium, Actinobacillus actinomycetemcomitans, was investigated using a chemostat. A. actinomycetemcomitans 301-b produced significant amounts of leukotoxin in anaerobic fructose-limited chemostat cultures at a dilution rate of 0.15 h(-1) and at pH 7.0. When the growth limitation was relieved by pulsing the cultures with 50 or 150 mM fructose (final concentrations), leukotoxin production immediately stopped and the amount of cellular leukotoxin decreased until the culture was returned to fructose-limited conditions. Leukotoxin synthesis was also repressed in the chemostat cultures by pulsing with glucose but not with the non-fermentable sugar analog, alpha-methyl-D-glucoside. Leukotoxin production was also repressed by fructose in chemostat cultures of ATCC 33384, which is generally recognized as a non-leukotoxin-producing or minimally leukotoxic strain.

    DOI: 10.1016/S0378-1097(96)00473-9

    Web of Science

    researchmap

  • Molecular cloning and characterization of the gene encoding 53 kD outer membrane protein of Porphyromonas gingivalis 査読 国際誌

    H Hongyo, H Kurihara, S Kokeguchi, M Miyamoto, H Maeda, M Hayakawa, Y Abiko, S Takashiba, Y Murayama

    MICROBIOS   92 ( 370 )   47 - 57   1997年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:FACULTY PRESS  

    The pga53 gene which encoded the antigenic 53 kD outer membrane protein (Ag53) was isolated from a genomic DNA library of Porphyromonas gingivalis FDC381 by using an Ag53-immunized rabbit serum. Determination of its complete nucleotide sequence revealed that the precursor of Ag53 had a 50 amino-acid putative signal sequence and the mature protein of 448 amino acids. The deduced amino acid sequence after a 50 amino-acid putative signal sequence was in complete agreement with the first 20 N-terminal amino acids of purified Ag53. Analysis of the deduced amino acid sequence revealed the presence of a highly hydrophilic proline-rich region at the C-terminal of Ag53. The deduced amino acid sequence showed 29.9% homology with that of a 72 kD cell-surface protein in P. gingivalis. Southern hybridization revealed that pga53 was specific to several P. gingivalis strains and that P. gingivalis strains which did not possess Ag53 had genes homologous to pga53.

    Web of Science

    PubMed

    researchmap

  • A regulatory gene controlling growth of Actinobacillus actinomycetemcomitans. 査読

    Kokeguchi S, Miyamoto M, Maeda H, Hongyo H, Hirosue M, Chou HH, Nishimura F, Arai H, Takashiba S, Murayama Y

    JOURNAL OF DENTAL RESEARCH   76   1683   1997年

  • Molecular and biological features of S-layer protein from Campylobacter rectus. 査読

    Maeda H, Kitanaka M, Kokeguchi S, Miyamoto M, Arai H, Nishimura F, Takashiba S, VanDyke TE, Murayama Y

    JOURNAL OF DENTAL RESEARCH   76   1687   1997年

  • Inhibition of nuclear factor kappa B subunit p65 mRNA accumulation in lipopolysaccharide-stimulated human monocytic cells treated with sodium salicylate 査読

    S Takashiba, TE VanDyke, S Amar

    ORAL MICROBIOLOGY AND IMMUNOLOGY   11 ( 6 )   420 - 424   1996年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MUNKSGAARD INT PUBL LTD  

    Lipopolysaccharide is one of the most potent trigger substances for monocytes and macrophages causing secretion of inflammatory mediators such as tumor necrosis factor and interleukin-1. The nature of the nuclear factors involved in regulation of these cytokine genes is still unknown. Nuclear factor kappa B (NF-kappa B: heterodimer of p50 and p65) proteins have been suggested to play an important role in gene transcription of inflammatory mediators when monocytes are stimulated with lipopolysaccharide. Nonsteroidal anti-inflammatory drugs such as salicylates have been used to treat symptoms of inflammation, and a new mechanism of drug action was suggested recently. Salicylates have been shown to inhibit lipopolysaccharide-induced gene transcription via inhibition of NF-kappa B activation by preventing the degradation of NF-kappa B inhibitor ''I kappa B'', blocking the translocation of NF-kappa B into the nuclear compartment. However, the nature of the subunit involved in this mechanism has not been defined. To examine the mechanisms by which salicylates affect cytokine gene transcription, the amount of active and inactive NF-kappa B and NF-kappa B mRNA, in Porphyromonas gingivalis lipopolysaccharide-stimulated human monocytic cells was assessed. High doses of sodium salicylate suppressed NF-kappa B p65 mRNA accumulation, resulting in suppression of total NF-kappa B, p50 on tissue oligonucleotide had no effects on lipopolysaccharide-induced NF-kappa B activation. The data demonstrate that the p65 subunit of NF-kappa B is inhibited by salicylate treatment and highlight the role of salicylate in the control of gene expression of inflammatory mediators.

    Web of Science

    researchmap

  • Inhibition of nuclear factor kappa B subunit p65 mRNA accumulation in lipopolysaccharide-stimulated human monocytic cells treated with sodium salicylate 査読 国際誌

    S Takashiba, TE VanDyke, S Amar

    ORAL MICROBIOLOGY AND IMMUNOLOGY   11 ( 6 )   420 - 424   1996年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MUNKSGAARD INT PUBL LTD  

    Lipopolysaccharide is one of the most potent trigger substances for monocytes and macrophages causing secretion of inflammatory mediators such as tumor necrosis factor and interleukin-1. The nature of the nuclear factors involved in regulation of these cytokine genes is still unknown. Nuclear factor kappa B (NF-kappa B: heterodimer of p50 and p65) proteins have been suggested to play an important role in gene transcription of inflammatory mediators when monocytes are stimulated with lipopolysaccharide. Nonsteroidal anti-inflammatory drugs such as salicylates have been used to treat symptoms of inflammation, and a new mechanism of drug action was suggested recently. Salicylates have been shown to inhibit lipopolysaccharide-induced gene transcription via inhibition of NF-kappa B activation by preventing the degradation of NF-kappa B inhibitor ''I kappa B'', blocking the translocation of NF-kappa B into the nuclear compartment. However, the nature of the subunit involved in this mechanism has not been defined. To examine the mechanisms by which salicylates affect cytokine gene transcription, the amount of active and inactive NF-kappa B and NF-kappa B mRNA, in Porphyromonas gingivalis lipopolysaccharide-stimulated human monocytic cells was assessed. High doses of sodium salicylate suppressed NF-kappa B p65 mRNA accumulation, resulting in suppression of total NF-kappa B, p50 on tissue oligonucleotide had no effects on lipopolysaccharide-induced NF-kappa B activation. The data demonstrate that the p65 subunit of NF-kappa B is inhibited by salicylate treatment and highlight the role of salicylate in the control of gene expression of inflammatory mediators.

    Web of Science

    PubMed

    researchmap

  • HLA class II genotypes associated with early-onset periodontitis: DQB1 molecule primarily confers susceptibility to the disease 査読 国際誌

    H Ohyama, S Takashiba, K Oyaizu, A Nagai, T Naruse, H Inoko, H Kurihara, Y Murayama

    JOURNAL OF PERIODONTOLOGY   67 ( 9 )   888 - 894   1996年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    DNA TYPING WAS PERFORMED on 24 Japanese patients with early-onset periodontitis (EOP) using the PCR-RFLP method to investigate an association of the susceptibility to EOP with the particular HLA class II alleles (HLA-DRB1, -DQA1, and -DQB1), DRB1*1401, DRB1*1501, DQB1*0503, and DQB1*0602 were found more frequently (''susceptible'') in the EOP patients than in healthy controls. In contrast, DRB1*0405 and DQB1*0401 were found less frequently (''resistant'') in EOP patients, All patients carrying DQB1*0602 had an atypical BamHI site in the intron upstream of the third exon of the DQB1 gene, which in our previous studies appeared to be a susceptible marker for EOP. A comparative analysis of the amino acid sequences of these susceptible and resistant HLA-DRB1 and DQB1 alleles elucidated some differences in antigen-derived peptide binding sites related to the susceptible or resistant alleles. Especially, DQB1*0503 and DQB1*0602 alleles carrying aspartic acid at position 57 and glycine at position 70 are increased significantly in EOP. Since amino acid residues at positions 57 and 70 on the DQB1 molecule are supposed to be involved in antigen binding, amino acid substitutions at these positions may affect the immune responsiveness to the periodontopathic antigen. Our results suggest that the DQB1 molecule plays a crucial role in the pathogenesis of EOP and that the susceptibility to EOP may be determined by the binding ability between the peptide and HLA-DQ antigens.

    Web of Science

    PubMed

    researchmap

  • HLA class II genotypes associated with early-onset periodontitis: DQB1 molecule primarily confers susceptibility to the disease 査読

    H Ohyama, S Takashiba, K Oyaizu, A Nagai, T Naruse, H Inoko, H Kurihara, Y Murayama

    JOURNAL OF PERIODONTOLOGY   67 ( 9 )   888 - 894   1996年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    DNA TYPING WAS PERFORMED on 24 Japanese patients with early-onset periodontitis (EOP) using the PCR-RFLP method to investigate an association of the susceptibility to EOP with the particular HLA class II alleles (HLA-DRB1, -DQA1, and -DQB1), DRB1*1401, DRB1*1501, DQB1*0503, and DQB1*0602 were found more frequently (''susceptible'') in the EOP patients than in healthy controls. In contrast, DRB1*0405 and DQB1*0401 were found less frequently (''resistant'') in EOP patients, All patients carrying DQB1*0602 had an atypical BamHI site in the intron upstream of the third exon of the DQB1 gene, which in our previous studies appeared to be a susceptible marker for EOP. A comparative analysis of the amino acid sequences of these susceptible and resistant HLA-DRB1 and DQB1 alleles elucidated some differences in antigen-derived peptide binding sites related to the susceptible or resistant alleles. Especially, DQB1*0503 and DQB1*0602 alleles carrying aspartic acid at position 57 and glycine at position 70 are increased significantly in EOP. Since amino acid residues at positions 57 and 70 on the DQB1 molecule are supposed to be involved in antigen binding, amino acid substitutions at these positions may affect the immune responsiveness to the periodontopathic antigen. Our results suggest that the DQB1 molecule plays a crucial role in the pathogenesis of EOP and that the susceptibility to EOP may be determined by the binding ability between the peptide and HLA-DQ antigens.

    Web of Science

    researchmap

  • Host defensive functions in a family manifesting early-onset periodontitis 査読

    H Arai, T Chihara, K Takahashi, A Nagai, Akutsu, I, S Takashiba, F Nishimura, H Kurihara, Y Murayama

    JOURNAL OF PERIODONTOLOGY   67 ( 4 )   433 - 442   1996年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    FAMILY CASE STUDIES HELP US IDENTIFY host risk factors in periodontal disease. In this study we examine a family consisting of a mother (40 years old, with rapidly progressive periodontitis), her elder daughter (14 years old, with localized juvenile periodontitis), and younger daughter (13 years old, with simple gingivitis). We examined 1) the peripheral neutrophil functions (chemotactic migration, phagocytosis, superoxide production); 2) lymphocyte functions (proliferative activity and cytokine productivity of T cells, immunoglobulin [Ig] M productivity of B cells when stimulated with pokeweed mitogen); 3) phenotypic analyses of peripheral lymphocyte subpopulations; 4) serum IgG antibody titers against periodontopathic bacteria; and 5) serological type of HLA class IS. All the subjects exhibited high T4/T8 ratios due to high percentage of CD4-positive cells, showed high IgG titers to Actinobacillus actinomycetemcomitans, and had a HLA DQw1 in common. The mother showed a slight deficiency of neutrophil chemotactic migration to N-formyl methyonyl leucyl phenylalanin (fMLP), raised interleukin-2 productivity of T cell, and high levels of IgG titers to Porphyromonus gingivalis and Fusobacterium nucleatum. Both daughters showed weak T cell proliferative response to anti-CD3 monoclonal antibody and low IgM productivity. Low lymphocyte responsiveness may be involved in the pathogenesis of periodontal disease of these daughters; therefore, the lymphocyte dysfunctions shown should be considered in relation to the progression of periodontal disease.

    Web of Science

    researchmap

  • Host defensive functions in a family manifesting early-onset periodontitis 査読 国際誌

    H Arai, T Chihara, K Takahashi, A Nagai, Akutsu, I, S Takashiba, F Nishimura, H Kurihara, Y Murayama

    JOURNAL OF PERIODONTOLOGY   67 ( 4 )   433 - 442   1996年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    FAMILY CASE STUDIES HELP US IDENTIFY host risk factors in periodontal disease. In this study we examine a family consisting of a mother (40 years old, with rapidly progressive periodontitis), her elder daughter (14 years old, with localized juvenile periodontitis), and younger daughter (13 years old, with simple gingivitis). We examined 1) the peripheral neutrophil functions (chemotactic migration, phagocytosis, superoxide production); 2) lymphocyte functions (proliferative activity and cytokine productivity of T cells, immunoglobulin [Ig] M productivity of B cells when stimulated with pokeweed mitogen); 3) phenotypic analyses of peripheral lymphocyte subpopulations; 4) serum IgG antibody titers against periodontopathic bacteria; and 5) serological type of HLA class IS. All the subjects exhibited high T4/T8 ratios due to high percentage of CD4-positive cells, showed high IgG titers to Actinobacillus actinomycetemcomitans, and had a HLA DQw1 in common. The mother showed a slight deficiency of neutrophil chemotactic migration to N-formyl methyonyl leucyl phenylalanin (fMLP), raised interleukin-2 productivity of T cell, and high levels of IgG titers to Porphyromonus gingivalis and Fusobacterium nucleatum. Both daughters showed weak T cell proliferative response to anti-CD3 monoclonal antibody and low IgM productivity. Low lymphocyte responsiveness may be involved in the pathogenesis of periodontal disease of these daughters; therefore, the lymphocyte dysfunctions shown should be considered in relation to the progression of periodontal disease.

    Web of Science

    PubMed

    researchmap

  • CLINICAL AND LABORATORY STUDIES ON A PATIENT WITH EARLY-ONSET PERIODONTITIS AND HER FAMILY MEMBERS - A CASE-REPORT 査読

    K TAKAHASHI, M TAKIGAWA, H HARA, A NAGAI, S TAKASHIBA, F NISHIMURA, T CHIHARA, H OHYAMA, N SATOH, H KURIHARA, Y MURAYAMA

    JOURNAL OF PERIODONTOLOGY   66 ( 5 )   403 - 412   1995年5月

     詳細を見る

    記述言語:英語   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    EXTENSIVE CLINICAL, MICROBIOLOGICAL, HEMATOLOGICAL, AND IMMUNOLOGICAL STUDIES were performed on a patient with early onset periodontitis (EOP) and two other members of the family. The proband, a 27-year-old female, had early onset periodontitis and a high level of serum rheumatoid factors (RF) with no diagnosable medical disease. Her mother had lost all her teeth at the age of 50 because of advanced periodontitis, while her elder sister was unaffected by periodontitis. Neither the proband's periodontally-affected mother nor her unaffected sister exhibited a detectable level of RE In this study, we examined: 1) serum immunoglobulin G (IgG) antibody titers against putative periodontal pathogenic bacteria; 2) peripheral neutrophil functions; 3) phenotypic analyses of peripheral lymphocyte subpopulations; and 4) peripheral lymphocyte functions (T cell proliferative activity, ability of cytokine [interleukin (IL)-2, tumor necrosis factor-alpha, interferon-gamma, IL-6 and IL-8] and IgG and IgM productivity). High antibody titers to Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Campylobacter rectus were detected in the sera of the proband, as were high serum antibody titers to P. gingivalis in the mother and to C. rectus in the unaffected sister compared to the non-periodontitis affected subjects. The proband also showed enhanced neutrophil chemotaxis; a high percentage of pan-B cells; and high productivity of IL-6, IgG, and IgM compared to individuals who were not periodontally affected. The mother showed slightly low helper/induced T cells (T-h/i) suppressor/cytotoxic T cells (T-s/c) ratios due to the elevated count of Ts/c, and high IFN-gamma productivity compared to control subjects. We could not identify any common risk factor in this family, but the immunological profiles of this proband might be useful for discussing the relationship between RF and periodontal disease.

    Web of Science

    researchmap

  • CLINICAL AND LABORATORY STUDIES ON A PATIENT WITH EARLY-ONSET PERIODONTITIS AND HER FAMILY MEMBERS - A CASE-REPORT 査読 国際誌

    K TAKAHASHI, M TAKIGAWA, H HARA, A NAGAI, S TAKASHIBA, F NISHIMURA, T CHIHARA, H OHYAMA, N SATOH, H KURIHARA, Y MURAYAMA

    JOURNAL OF PERIODONTOLOGY   66 ( 5 )   403 - 412   1995年5月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    EXTENSIVE CLINICAL, MICROBIOLOGICAL, HEMATOLOGICAL, AND IMMUNOLOGICAL STUDIES were performed on a patient with early onset periodontitis (EOP) and two other members of the family. The proband, a 27-year-old female, had early onset periodontitis and a high level of serum rheumatoid factors (RF) with no diagnosable medical disease. Her mother had lost all her teeth at the age of 50 because of advanced periodontitis, while her elder sister was unaffected by periodontitis. Neither the proband's periodontally-affected mother nor her unaffected sister exhibited a detectable level of RE In this study, we examined: 1) serum immunoglobulin G (IgG) antibody titers against putative periodontal pathogenic bacteria; 2) peripheral neutrophil functions; 3) phenotypic analyses of peripheral lymphocyte subpopulations; and 4) peripheral lymphocyte functions (T cell proliferative activity, ability of cytokine [interleukin (IL)-2, tumor necrosis factor-alpha, interferon-gamma, IL-6 and IL-8] and IgG and IgM productivity). High antibody titers to Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Campylobacter rectus were detected in the sera of the proband, as were high serum antibody titers to P. gingivalis in the mother and to C. rectus in the unaffected sister compared to the non-periodontitis affected subjects. The proband also showed enhanced neutrophil chemotaxis; a high percentage of pan-B cells; and high productivity of IL-6, IgG, and IgM compared to individuals who were not periodontally affected. The mother showed slightly low helper/induced T cells (T-h/i) suppressor/cytotoxic T cells (T-s/c) ratios due to the elevated count of Ts/c, and high IFN-gamma productivity compared to control subjects. We could not identify any common risk factor in this family, but the immunological profiles of this proband might be useful for discussing the relationship between RF and periodontal disease.

    Web of Science

    PubMed

    researchmap

  • LIPOPOLYSACCHARIDE-INDUCIBLE AND SALICYLATE-SENSITIVE NUCLEAR FACTOR(S) ON HUMAN TUMOR-NECROSIS-FACTOR-ALPHA PROMOTER 査読

    S TAKASHIBA, TE VANDYKE, L SHAPIRA, S AMAR

    INFECTION AND IMMUNITY   63 ( 4 )   1529 - 1534   1995年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    Lipopolysaccharide (LPS) is one of the most potent trigger substances for monocytes and macrophages causing secretion of tumor necrosis factor alpha (TNF-alpha) and other inflammatory mediators, The nature of the nuclear factors involved in human TNF-alpha gene regulation is still unknown. Nuclear factor kappa B (NF-kappa B) proteins have been suggested to play an important role in gene transcription of inflammatory mediators when monocytes are stimulated with LPS. However, it remains unclear whether these nuclear factors are the only ones involved in human TNF-alpha gene regulation. In this report, to further the identification of nuclear factor(s) involved in TNF-alpha gene regulation, human monocytic THP-1 cells were transfected with a series of truncated versions of human TNF-alpha promoter. A 98-bp region located from nucleotides -584 to -487 demonstrated strong promoter activity, Electrophoretic mobility shift assays demonstrated that a 64-bp fragment located within the 98-bp region and lacking any potential NP-kappa B-binding sites avidly bound LPS-challenged THP-1 nuclear protein, Although this binding was inhibited in salicylate-treated cells, as was binding of NF-kappa B, the pattern of binding was found to differ from that noted for NF-kappa B. Analysis of this 64-bp fragment disclosed the absence of an NF-kappa B consensus sequence, suggesting a novel nuclear DNA-binding protein necessary for the initiation of human TNF-alpha transcription other than, or in addition to, NF-kappa B.

    Web of Science

    researchmap

  • LIPOPOLYSACCHARIDE-INDUCIBLE AND SALICYLATE-SENSITIVE NUCLEAR FACTOR(S) ON HUMAN TUMOR-NECROSIS-FACTOR-ALPHA PROMOTER 査読 国際誌

    S TAKASHIBA, TE VANDYKE, L SHAPIRA, S AMAR

    INFECTION AND IMMUNITY   63 ( 4 )   1529 - 1534   1995年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    Lipopolysaccharide (LPS) is one of the most potent trigger substances for monocytes and macrophages causing secretion of tumor necrosis factor alpha (TNF-alpha) and other inflammatory mediators, The nature of the nuclear factors involved in human TNF-alpha gene regulation is still unknown. Nuclear factor kappa B (NF-kappa B) proteins have been suggested to play an important role in gene transcription of inflammatory mediators when monocytes are stimulated with LPS. However, it remains unclear whether these nuclear factors are the only ones involved in human TNF-alpha gene regulation. In this report, to further the identification of nuclear factor(s) involved in TNF-alpha gene regulation, human monocytic THP-1 cells were transfected with a series of truncated versions of human TNF-alpha promoter. A 98-bp region located from nucleotides -584 to -487 demonstrated strong promoter activity, Electrophoretic mobility shift assays demonstrated that a 64-bp fragment located within the 98-bp region and lacking any potential NP-kappa B-binding sites avidly bound LPS-challenged THP-1 nuclear protein, Although this binding was inhibited in salicylate-treated cells, as was binding of NF-kappa B, the pattern of binding was found to differ from that noted for NF-kappa B. Analysis of this 64-bp fragment disclosed the absence of an NF-kappa B consensus sequence, suggesting a novel nuclear DNA-binding protein necessary for the initiation of human TNF-alpha transcription other than, or in addition to, NF-kappa B.

    Web of Science

    PubMed

    researchmap

  • PROSTAGLANDIN E(2) INHIBITS INTERLEUKIN-6 RELEASE BUT NOT ITS TRANSCRIPTION IN HUMAN GINGIVAL FIBROBLASTS STIMULATED WITH INTERLEUKIN-1-BETA OR TUMOR-NECROSIS-FACTOR-ALPHA 査読

    M TAKIGAWA, S TAKASHIBA, K TAKAHASHI, H ARAI, H KURIHARA, Y MURAYAMA

    JOURNAL OF PERIODONTOLOGY   65 ( 12 )   1122 - 1127   1994年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    INFLAMMATORY MEDIATORS PRODUCED BY HUMAN GINGIVAL FIBROBLASTS (HGF) have been implicated in the initiation and progression of periodontal disease. The purpose of this study was to examine whether prostaglandin E(2) (PGE(2)), which is produced in abundance from HGF after stimulation with interleukin (IL)-1 beta or tumor necrosis factor-alpha (TNF-alpha), could regulate IL-6 production by HGF. HGF stimulated with either IL-1 beta or TNF-alpha showed a rapid and dose-dependent increase in IL-6 mRNA accumulation and IL-6 secretion, as demonstrated by reverse transcription-polymerase chain reaction analysis and bioassay. IL-6 secretion from either IL-1 beta- or TNF-alpha-stimulated HGF was enhanced by the inhibition of PGE(2) synthesis with indomethacin. Furthermore, the addition of PGE(2) inhibited IL-6 secretion from these cells. In contrast, indomethacin or PGE(2) did not affect the accumulation of IL-6 mRNA in IL-1 beta-stimulated HGF. These data indicate that IL-6 production by HGF is up-regulated by specific cytokines, IL-1 beta and TNF-alpha, and suggest that this production may be partially down-regulated by endogenous and exogenous PGE(2) at the post-transcriptional level.

    Web of Science

    researchmap

  • PROSTAGLANDIN E(2) INHIBITS INTERLEUKIN-6 RELEASE BUT NOT ITS TRANSCRIPTION IN HUMAN GINGIVAL FIBROBLASTS STIMULATED WITH INTERLEUKIN-1-BETA OR TUMOR-NECROSIS-FACTOR-ALPHA 査読

    M TAKIGAWA, S TAKASHIBA, K TAKAHASHI, H ARAI, H KURIHARA, Y MURAYAMA

    JOURNAL OF PERIODONTOLOGY   65 ( 12 )   1122 - 1127   1994年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    INFLAMMATORY MEDIATORS PRODUCED BY HUMAN GINGIVAL FIBROBLASTS (HGF) have been implicated in the initiation and progression of periodontal disease. The purpose of this study was to examine whether prostaglandin E(2) (PGE(2)), which is produced in abundance from HGF after stimulation with interleukin (IL)-1 beta or tumor necrosis factor-alpha (TNF-alpha), could regulate IL-6 production by HGF. HGF stimulated with either IL-1 beta or TNF-alpha showed a rapid and dose-dependent increase in IL-6 mRNA accumulation and IL-6 secretion, as demonstrated by reverse transcription-polymerase chain reaction analysis and bioassay. IL-6 secretion from either IL-1 beta- or TNF-alpha-stimulated HGF was enhanced by the inhibition of PGE(2) synthesis with indomethacin. Furthermore, the addition of PGE(2) inhibited IL-6 secretion from these cells. In contrast, indomethacin or PGE(2) did not affect the accumulation of IL-6 mRNA in IL-1 beta-stimulated HGF. These data indicate that IL-6 production by HGF is up-regulated by specific cytokines, IL-1 beta and TNF-alpha, and suggest that this production may be partially down-regulated by endogenous and exogenous PGE(2) at the post-transcriptional level.

    Web of Science

    PubMed

    researchmap

  • CYTOKINE-DEPENDENT SYNERGISTIC REGULATION OF INTERLEUKIN-8 PRODUCTION FROM HUMAN GINGIVAL FIBROBLASTS 査読

    M TAKIGAWA, S TAKASHIBA, F MYOKAI, L TAKAHASHI, H ARAI, H KURIHARA, Y MURAYAMA

    JOURNAL OF PERIODONTOLOGY   65 ( 11 )   1002 - 1007   1994年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    HUMAN GINGIVAL FIBROBLASTS (HGF) may have an important role in the orchestration of immuno participant cells infiltrating the gingiva in response to continuously recurring bacterial infection. To examine the cytokine network regulating HGF-derived interleukin (IL)-8, a potent neutrophil chemotactic cytokine, we analyzed the effects of inflammatory cytokines alone and iii combination on IL-8 production by HGF. IL-1 beta, tumor necrosis factor-alpha (TNF-alpha), Interferon-gamma (IFN-gamma), IL-6, and IL-8 were used as stimulants. HGF secreted IL-8 in a dose-dependent manner after stimulation with either IL-1 beta or TNF-alpha, but not with IFN-gamma or IL-6. Furthermore, IL-8 itself did not affect IL-8 mRNA accumulation in HGF in an autocrine manner, The combination of IL-1 beta and TNF-alpha synergistically enhanced the secretion of IL-8, whereas IFN-gamma suppressed IL-8 secretion by IL-1 beta- or TNF-alpha-stimulated HGF. These effects were also observed at each level of IL-8 mRNA expression in HGF. IL-8 secretion by cytokine-stimulated HGF was not influenced my the inhibition of PGE(2) synthesis with indomethacin, indicating that endogenous PGE(2) was not involved in IL-8 production by HGF. These results indicate that IL-8 production by HGF is synergistically stimulated by specific cytokines, IL-1 beta and TNF-alpha, and suggest that these stimulatory effects are down-regulated by IFN-gamma at the transcriptional let ei through PGE(2)-independent pathways. Thus, neutrophil-mediated processes in periodontal disease may be regulated in part by HGF in the cytokine network of immuno-participant cells.

    Web of Science

    researchmap

  • CYTOKINE-DEPENDENT SYNERGISTIC REGULATION OF INTERLEUKIN-8 PRODUCTION FROM HUMAN GINGIVAL FIBROBLASTS 査読 国際誌

    M TAKIGAWA, S TAKASHIBA, F MYOKAI, L TAKAHASHI, H ARAI, H KURIHARA, Y MURAYAMA

    JOURNAL OF PERIODONTOLOGY   65 ( 11 )   1002 - 1007   1994年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    HUMAN GINGIVAL FIBROBLASTS (HGF) may have an important role in the orchestration of immuno participant cells infiltrating the gingiva in response to continuously recurring bacterial infection. To examine the cytokine network regulating HGF-derived interleukin (IL)-8, a potent neutrophil chemotactic cytokine, we analyzed the effects of inflammatory cytokines alone and iii combination on IL-8 production by HGF. IL-1 beta, tumor necrosis factor-alpha (TNF-alpha), Interferon-gamma (IFN-gamma), IL-6, and IL-8 were used as stimulants. HGF secreted IL-8 in a dose-dependent manner after stimulation with either IL-1 beta or TNF-alpha, but not with IFN-gamma or IL-6. Furthermore, IL-8 itself did not affect IL-8 mRNA accumulation in HGF in an autocrine manner, The combination of IL-1 beta and TNF-alpha synergistically enhanced the secretion of IL-8, whereas IFN-gamma suppressed IL-8 secretion by IL-1 beta- or TNF-alpha-stimulated HGF. These effects were also observed at each level of IL-8 mRNA expression in HGF. IL-8 secretion by cytokine-stimulated HGF was not influenced my the inhibition of PGE(2) synthesis with indomethacin, indicating that endogenous PGE(2) was not involved in IL-8 production by HGF. These results indicate that IL-8 production by HGF is synergistically stimulated by specific cytokines, IL-1 beta and TNF-alpha, and suggest that these stimulatory effects are down-regulated by IFN-gamma at the transcriptional let ei through PGE(2)-independent pathways. Thus, neutrophil-mediated processes in periodontal disease may be regulated in part by HGF in the cytokine network of immuno-participant cells.

    Web of Science

    PubMed

    researchmap

  • MOLECULAR-BASIS OF LEUKOCYTE ADHESION MOLECULES IN EARLY-ONSET PERIODONTITIS PATIENTS WITH DECREASED CD11/CD18 EXPRESSION ON LEUKOCYTES 査読 国際誌

    K KATSURAGI, S TAKASHIBA, H KURIHARA, Y MURAYAMA

    JOURNAL OF PERIODONTOLOGY   65 ( 10 )   949 - 957   1994年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    WE ANALYZED THE CELL-CELL ADHERENCE RELATED to CD11/CD18 and CD18 mRNA in individuals with decreased CD11/CD18 expression on their neutrophil surface. Epstein Barr virus-transformed B cell lines were developed from one localized juvenile periodontitis (LJP) patient with decreased CD11/CD18 in the peripheral blood neutrophils and without systemic diseases; two siblings with generalized prepubertal periodontitis (GPP) caused by leukocyte adhesion deficiency (LAD); another LJP patient; one localized prepubertal periodontitis (LPP) patient; and two healthy subjects. Adhesion of leukocytes to each other was measured as cluster formation by aggregation assay. The length and the amount of CD18 mRNA expressed in the cell lines were analyzed by Northern blotting using the P-32-labeled CD18 cDNA. The coding region of the mRNA was analyzed by the reverse transcription-polymerase chain reaction method. Base-mismatches between CD18 mRNA and the P-32-labeled RNA probe synthesized from CD18 cDNA were analyzed by RNase protection assay. In the adherence assay, cells from the LJP patients with decreased CD11/CD18 formed more clusters of smaller size and fewer cells than those of the other subjects. The cells from GPP and LAD patients did not aggregate and did not form clusters either in the absence or presence of PMA. There were no differences in the length and the amount of mRNA between the LJP patients and the other subjects, while GPP-LAD patients expressed a small amount of long mRNA. The whole coding region (2,313 base pairs) of all subjects was amplified except for the GPP-LAD patients, and the 5'-region (1,119 base pairs) was amplified from all subjects. Base-mismatches were detected on the coding region from 965 to 1,450 nucleotides of CD18 mRNA in GPP-LAD patients. No mismatch was detected on other regions of CD18 mRNA in any subject. These results suggest that the LJP patient with an anomaly of qualitative aggregation related to CD11/CD18 is not related to sequence abnormalities of CD18 mRNA suggesting other mechanisms. In contrast, the adherence defect in two GPP-LAD patients was related to heterogeneous molecular mutations on CD18.

    Web of Science

    PubMed

    researchmap

  • MOLECULAR-BASIS OF LEUKOCYTE ADHESION MOLECULES IN EARLY-ONSET PERIODONTITIS PATIENTS WITH DECREASED CD11/CD18 EXPRESSION ON LEUKOCYTES 査読

    K KATSURAGI, S TAKASHIBA, H KURIHARA, Y MURAYAMA

    JOURNAL OF PERIODONTOLOGY   65 ( 10 )   949 - 957   1994年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    WE ANALYZED THE CELL-CELL ADHERENCE RELATED to CD11/CD18 and CD18 mRNA in individuals with decreased CD11/CD18 expression on their neutrophil surface. Epstein Barr virus-transformed B cell lines were developed from one localized juvenile periodontitis (LJP) patient with decreased CD11/CD18 in the peripheral blood neutrophils and without systemic diseases; two siblings with generalized prepubertal periodontitis (GPP) caused by leukocyte adhesion deficiency (LAD); another LJP patient; one localized prepubertal periodontitis (LPP) patient; and two healthy subjects. Adhesion of leukocytes to each other was measured as cluster formation by aggregation assay. The length and the amount of CD18 mRNA expressed in the cell lines were analyzed by Northern blotting using the P-32-labeled CD18 cDNA. The coding region of the mRNA was analyzed by the reverse transcription-polymerase chain reaction method. Base-mismatches between CD18 mRNA and the P-32-labeled RNA probe synthesized from CD18 cDNA were analyzed by RNase protection assay. In the adherence assay, cells from the LJP patients with decreased CD11/CD18 formed more clusters of smaller size and fewer cells than those of the other subjects. The cells from GPP and LAD patients did not aggregate and did not form clusters either in the absence or presence of PMA. There were no differences in the length and the amount of mRNA between the LJP patients and the other subjects, while GPP-LAD patients expressed a small amount of long mRNA. The whole coding region (2,313 base pairs) of all subjects was amplified except for the GPP-LAD patients, and the 5'-region (1,119 base pairs) was amplified from all subjects. Base-mismatches were detected on the coding region from 965 to 1,450 nucleotides of CD18 mRNA in GPP-LAD patients. No mismatch was detected on other regions of CD18 mRNA in any subject. These results suggest that the LJP patient with an anomaly of qualitative aggregation related to CD11/CD18 is not related to sequence abnormalities of CD18 mRNA suggesting other mechanisms. In contrast, the adherence defect in two GPP-LAD patients was related to heterogeneous molecular mutations on CD18.

    Web of Science

    researchmap

  • INVOLVEMENT OF PROTEIN-KINASE-C AND PROTEIN-TYROSINE KINASE IN LIPOPOLYSACCHARIDE-INDUCED TNF-ALPHA AND IL-1-BETA PRODUCTION BY HUMAN MONOCYTES 査読 国際誌

    L SHAPIRA, S TAKASHIBA, C CHAMPAGNE, S AMAR, TE VANDYKE

    JOURNAL OF IMMUNOLOGY   153 ( 4 )   1818 - 1824   1994年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC IMMUNOLOGISTS  

    Bacterial LPS stimulates human monocytes to secrete inflammatory cytokines, which are involved in several disease processes. However, the mechanism of LPS activation of cytokine expression and secretion is not completely understood. In this study, we investigated the signal transduction pathways involved in LPS-stimulated TNF-alpha and IL-1 beta secretion. TNF-alpha and IL-1 beta secretion were completely blocked by protein kinase C (PKC) and cyclic nucleotide-dependent protein kinase inhibitor, H-7, but were not affected by H-89, a specific cyclic nucleotide-dependent protein kinase inhibitor. In addition, LPS was found to induce activation of PKC, reaching maximal activity at 30 min and returning to unstimulated levels after 60 min. LPS stimulation only slightly increased intracellular levels of diacylglycerol, the natural activator of PKC, and pretreatment of monocytes with the diacylglycerol-kinase inhibitor, R59022, did not affect LPS-stimulated TNF-alpha secretion. LPS-induced PKC activation was found not to be affected by blocking of the LPS receptor, CD14, with mAb or by inhibition of protein tyrosine kinase with herbimycin A. However, these agents suppressed LPS-induced TNF-alpha secretion and TNF-alpha mRNA accumulation. The results suggest that TNF-alpha and IL-1 beta secretion after LPS stimulation of human monocytes requires the activation of protein tyrosine kinase and PKC, upstream to the activation of gene transcription. The activation of PKC by LPS is probably mediated by a diacylglycerol-independent pathway.

    Web of Science

    PubMed

    researchmap

  • INVOLVEMENT OF PROTEIN-KINASE-C AND PROTEIN-TYROSINE KINASE IN LIPOPOLYSACCHARIDE-INDUCED TNF-ALPHA AND IL-1-BETA PRODUCTION BY HUMAN MONOCYTES 査読

    L SHAPIRA, S TAKASHIBA, C CHAMPAGNE, S AMAR, TE VANDYKE

    JOURNAL OF IMMUNOLOGY   153 ( 4 )   1818 - 1824   1994年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC IMMUNOLOGISTS  

    Bacterial LPS stimulates human monocytes to secrete inflammatory cytokines, which are involved in several disease processes. However, the mechanism of LPS activation of cytokine expression and secretion is not completely understood. In this study, we investigated the signal transduction pathways involved in LPS-stimulated TNF-alpha and IL-1 beta secretion. TNF-alpha and IL-1 beta secretion were completely blocked by protein kinase C (PKC) and cyclic nucleotide-dependent protein kinase inhibitor, H-7, but were not affected by H-89, a specific cyclic nucleotide-dependent protein kinase inhibitor. In addition, LPS was found to induce activation of PKC, reaching maximal activity at 30 min and returning to unstimulated levels after 60 min. LPS stimulation only slightly increased intracellular levels of diacylglycerol, the natural activator of PKC, and pretreatment of monocytes with the diacylglycerol-kinase inhibitor, R59022, did not affect LPS-stimulated TNF-alpha secretion. LPS-induced PKC activation was found not to be affected by blocking of the LPS receptor, CD14, with mAb or by inhibition of protein tyrosine kinase with herbimycin A. However, these agents suppressed LPS-induced TNF-alpha secretion and TNF-alpha mRNA accumulation. The results suggest that TNF-alpha and IL-1 beta secretion after LPS stimulation of human monocytes requires the activation of protein tyrosine kinase and PKC, upstream to the activation of gene transcription. The activation of PKC by LPS is probably mediated by a diacylglycerol-independent pathway.

    Web of Science

    researchmap

  • AN ATYPICAL SITE IN HLA-DQB1 DETECTED IN LEPROSY PATIENTS 査読 国際誌

    H OHYAMA, A NAGAI, S TAKASHIBA, K SUGIYAMA, S INOUE, M MIZUSHIMA, A KOHZUMA, Y MURAYAMA

    INTERNATIONAL JOURNAL OF LEPROSY AND OTHER MYCOBACTERIAL DISEASES   62 ( 2 )   293 - 294   1994年6月

     詳細を見る

    記述言語:英語   出版者・発行元:AMER LEPROSY MISSION  

    Web of Science

    PubMed

    researchmap

  • AN ATYPICAL SITE IN HLA-DQB1 DETECTED IN LEPROSY PATIENTS 査読

    H OHYAMA, A NAGAI, S TAKASHIBA, K SUGIYAMA, S INOUE, M MIZUSHIMA, A KOHZUMA, Y MURAYAMA

    INTERNATIONAL JOURNAL OF LEPROSY AND OTHER MYCOBACTERIAL DISEASES   62 ( 2 )   293 - 294   1994年6月

     詳細を見る

    記述言語:英語   出版者・発行元:AMER LEPROSY MISSION  

    Web of Science

    researchmap

  • UNIQUE INTRONIC VARIATIONS OF HLA-DQ-BETA GENE IN EARLY-ONSET PERIODONTITIS 査読 国際誌

    S TAKASHIBA, S NOJI, F NISHIMURA, H OHYAMA, H KURIHARA, Y NOMURA, S TANIGUCHI, Y MURAYAMA

    JOURNAL OF PERIODONTOLOGY   65 ( 5 )   379 - 386   1994年5月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    HUMAN LEUKOCYTE ANTIGEN (HLA) class II beta chain plays an important role in the recognition of foreign antigens in immune reactions. Different forms of immune reaction may be concerned with initiation and progression of infectious diseases such as periodontitis. In this study we examined the frequency of HLA class II serotype and the variation of HLA class II beta gene in periodontitis patients. HLA serotypic frequencies in 70 Japanese patients with periodontitis and 26 individuals with periodontal health were examined. No HLA serotype specific to any type of periodontitis was observed. In order to detect differences among some HLA serotypes, restriction fragment length polymorphism (RFLP) analysis was undertaken with cDNA probes for HLA-DR beta and HLA-DQ beta genes in 20 subjects (15 patients and 5 healthy individuals). Atypical BamHI and EcoRI restriction sites were found in the HLA-DQ beta gene from 3 patients with early-onset periodontitis. In addition to these 20 subjects, an additional 80 subjects (40 patients and 40 healthy individuals) were screened for the atypical BamHI restriction site using the polymerase chain reaction method. It was detected in 7 patients with early-onset periodontitis, 1 patient with adult periodontitis, and 3 healthy subjects. No clinical differences except age were found between patients with this gene variation and other patients. Interestingly, all 3 healthy subjects with this gene variation were from subjects whose family members developed early-onset periodontitis with the gene variation. Atypical BamHI and EcoRI restriction sites and 41-nt repeated sequence were found in the intron before the third exon of HLA-DQB gene. These results suggest that these intronic gene variations may be useful as gene markers for a subpopulation of early-onset periodontitis and might affect immune reactions such as antigen recognition.

    Web of Science

    PubMed

    researchmap

  • UNIQUE INTRONIC VARIATIONS OF HLA-DQ-BETA GENE IN EARLY-ONSET PERIODONTITIS 査読

    S TAKASHIBA, S NOJI, F NISHIMURA, H OHYAMA, H KURIHARA, Y NOMURA, S TANIGUCHI, Y MURAYAMA

    JOURNAL OF PERIODONTOLOGY   65 ( 5 )   379 - 386   1994年5月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    HUMAN LEUKOCYTE ANTIGEN (HLA) class II beta chain plays an important role in the recognition of foreign antigens in immune reactions. Different forms of immune reaction may be concerned with initiation and progression of infectious diseases such as periodontitis. In this study we examined the frequency of HLA class II serotype and the variation of HLA class II beta gene in periodontitis patients. HLA serotypic frequencies in 70 Japanese patients with periodontitis and 26 individuals with periodontal health were examined. No HLA serotype specific to any type of periodontitis was observed. In order to detect differences among some HLA serotypes, restriction fragment length polymorphism (RFLP) analysis was undertaken with cDNA probes for HLA-DR beta and HLA-DQ beta genes in 20 subjects (15 patients and 5 healthy individuals). Atypical BamHI and EcoRI restriction sites were found in the HLA-DQ beta gene from 3 patients with early-onset periodontitis. In addition to these 20 subjects, an additional 80 subjects (40 patients and 40 healthy individuals) were screened for the atypical BamHI restriction site using the polymerase chain reaction method. It was detected in 7 patients with early-onset periodontitis, 1 patient with adult periodontitis, and 3 healthy subjects. No clinical differences except age were found between patients with this gene variation and other patients. Interestingly, all 3 healthy subjects with this gene variation were from subjects whose family members developed early-onset periodontitis with the gene variation. Atypical BamHI and EcoRI restriction sites and 41-nt repeated sequence were found in the intron before the third exon of HLA-DQB gene. These results suggest that these intronic gene variations may be useful as gene markers for a subpopulation of early-onset periodontitis and might affect immune reactions such as antigen recognition.

    Web of Science

    researchmap

  • PORPHYROMONAS-GINGIVALIS LIPOPOLYSACCHARIDE STIMULATION OF HUMAN MONOCYTES - DEPENDENCE ON SERUM AND CD14-RECEPTOR 査読

    L SHAPIRA, S TAKASHIBA, S AMAR, TE VANDYKE

    ORAL MICROBIOLOGY AND IMMUNOLOGY   9 ( 2 )   112 - 117   1994年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MUNKSGAARD INT PUBL LTD  

    The purpose of this study was to investigate factors influencing the ability of lipopolysaccharide (LPS) derived from Porphyromonas gingivalis to elicit secretion of tumor necrosis factor-alpha (TNFalpha) from human monocytes (adherent mononuclear cells). The results indicate that P gingivalis LPS stimulation of TNFalpha from monocytes is comparable to LPS from Escherichia coli. Both LPS, although structurally different, increased TNFalpha secretion in a dose-dependent manner. In serum-free conditions, TNFalpha secretion was relatively low, but it dramatically increased at human serum concentrations as low as 1%. Maximal secretion was observed in the presence of 10% serum, with a slight decrease at higher serum concentrations. The CD14 molecule is a putative monocyte LPS receptor. When cells were pre-incubated with a blocking monoclonal antibody (My4) to CD14, TNFalpha-mRNA accumulation and TNFalpha secretion were reduced to control levels at LPS concentrations of up to 10 ng/ml. At higher LPS concentrations, the blocking effect was only partial, in spite of 50-fold excess antibody concentration. The blocking effect was observed only in the presence of serum. The effect of the CD14 antibody was dose-dependent with saturation at 2.5 mug/ml. The results suggest that CD14 is one of the major receptors for P gingivalis LPS but highlight the necessity to investigate other cell-surface receptors mediating P gingivalis-LPS interactions. These interactions are believed to be important in the pathogenesis of periodontal destruction.

    Web of Science

    researchmap

  • PORPHYROMONAS-GINGIVALIS LIPOPOLYSACCHARIDE STIMULATION OF HUMAN MONOCYTES - DEPENDENCE ON SERUM AND CD14-RECEPTOR 査読 国際誌

    L SHAPIRA, S TAKASHIBA, S AMAR, TE VANDYKE

    ORAL MICROBIOLOGY AND IMMUNOLOGY   9 ( 2 )   112 - 117   1994年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MUNKSGAARD INT PUBL LTD  

    The purpose of this study was to investigate factors influencing the ability of lipopolysaccharide (LPS) derived from Porphyromonas gingivalis to elicit secretion of tumor necrosis factor-alpha (TNFalpha) from human monocytes (adherent mononuclear cells). The results indicate that P gingivalis LPS stimulation of TNFalpha from monocytes is comparable to LPS from Escherichia coli. Both LPS, although structurally different, increased TNFalpha secretion in a dose-dependent manner. In serum-free conditions, TNFalpha secretion was relatively low, but it dramatically increased at human serum concentrations as low as 1%. Maximal secretion was observed in the presence of 10% serum, with a slight decrease at higher serum concentrations. The CD14 molecule is a putative monocyte LPS receptor. When cells were pre-incubated with a blocking monoclonal antibody (My4) to CD14, TNFalpha-mRNA accumulation and TNFalpha secretion were reduced to control levels at LPS concentrations of up to 10 ng/ml. At higher LPS concentrations, the blocking effect was only partial, in spite of 50-fold excess antibody concentration. The blocking effect was observed only in the presence of serum. The effect of the CD14 antibody was dose-dependent with saturation at 2.5 mug/ml. The results suggest that CD14 is one of the major receptors for P gingivalis LPS but highlight the necessity to investigate other cell-surface receptors mediating P gingivalis-LPS interactions. These interactions are believed to be important in the pathogenesis of periodontal destruction.

    Web of Science

    PubMed

    researchmap

  • ROLE OF CYTOKINE IN THE INDUCTION OF ADHESION MOLECULES ON CULTURED HUMAN GINGIVAL FIBROBLASTS 査読

    K TAKAHASHI, M TAKIGAWA, S TAKASHIBA, A NAGAI, M MIYAMOTO, H KURIHARA, Y MURAYAMA

    JOURNAL OF PERIODONTOLOGY   65 ( 3 )   230 - 235   1994年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    THIS STUDY WAS UNDERTAKEN IN AN EFFORT to understand the role of cytokines on human gingival fibroblasts and T lymphocyte trafficing into inflamed gingival tissue. Using flow cytometry we examined gingival fibroblasts to determine the level of cell surface expression and the percentage of cells positive for intercellular adhesion molecule 1 (ICAM-1), the HLA-DR antigen, lymphocyte function-associated antigen 3 (LFA-3), and the CD44 molecule, which are involved in antigen presentation. The following cytokines were used: interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), IL-6, and IL-8. The levels of ICAM-1 expression were enhanced in a dose- and time-dependent manner by IL-1 beta, TNF-alpha, or IFN-gamma, but not by IL-6 or IL-8. HLA-DR surface expression was induced only by IFN-gamma in a dose- and time-dependent manner, but not by the other cytokines tested. In contrast, the expression of LFA3 and the CD44 molecule could be detected without the stimulation of any cytokine, but the levels of their expression were not significantly changed by any cytokines. The enhanced ICAM-1 expression by cytokines was reduced in a time-dependent manner following the removal of cytokines from the reaction mixture, while IFN-gamma-induced HLA-DR expression was maintained even 7 days after the removal of IFN-gamma. These data support an interactive role for inflammatory cytokines and the expression of adhesion molecules on gingival fibroblasts in the pathogenesis of gingival inflammation in periodontal disease.

    Web of Science

    researchmap

  • ROLE OF CYTOKINE IN THE INDUCTION OF ADHESION MOLECULES ON CULTURED HUMAN GINGIVAL FIBROBLASTS 査読 国際誌

    K TAKAHASHI, M TAKIGAWA, S TAKASHIBA, A NAGAI, M MIYAMOTO, H KURIHARA, Y MURAYAMA

    JOURNAL OF PERIODONTOLOGY   65 ( 3 )   230 - 235   1994年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    THIS STUDY WAS UNDERTAKEN IN AN EFFORT to understand the role of cytokines on human gingival fibroblasts and T lymphocyte trafficing into inflamed gingival tissue. Using flow cytometry we examined gingival fibroblasts to determine the level of cell surface expression and the percentage of cells positive for intercellular adhesion molecule 1 (ICAM-1), the HLA-DR antigen, lymphocyte function-associated antigen 3 (LFA-3), and the CD44 molecule, which are involved in antigen presentation. The following cytokines were used: interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), IL-6, and IL-8. The levels of ICAM-1 expression were enhanced in a dose- and time-dependent manner by IL-1 beta, TNF-alpha, or IFN-gamma, but not by IL-6 or IL-8. HLA-DR surface expression was induced only by IFN-gamma in a dose- and time-dependent manner, but not by the other cytokines tested. In contrast, the expression of LFA3 and the CD44 molecule could be detected without the stimulation of any cytokine, but the levels of their expression were not significantly changed by any cytokines. The enhanced ICAM-1 expression by cytokines was reduced in a time-dependent manner following the removal of cytokines from the reaction mixture, while IFN-gamma-induced HLA-DR expression was maintained even 7 days after the removal of IFN-gamma. These data support an interactive role for inflammatory cytokines and the expression of adhesion molecules on gingival fibroblasts in the pathogenesis of gingival inflammation in periodontal disease.

    Web of Science

    PubMed

    researchmap

  • ASSESSMENT OF INTERLEUKIN-6 IN THE PATHOGENESIS OF PERIODONTAL-DISEASE 査読

    K TAKAHASHI, S TAKASHIBA, A NAGAI, M TAKIGAWA, F MYOUKAI, H KURIHARA, Y MURAYAMA

    JOURNAL OF PERIODONTOLOGY   65 ( 2 )   147 - 153   1994年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    THIS STUDY WAS PERFORMED TO INVESTIGATE the aspects of interleukin-6 (IL-6) production in both the gingival tissue and the peripheral blood of patients with periodontal disease and of periodontally healthy subjects. In addition, IL-6 expression in human gingival tissues was studied by reverse transcription-polymerase chain reaction analysis and by immunoperoxidase staining with anti-IL-6 monoclonal antibody. The levels of IL-6 in the culture supernatants from peripheral blood mononuclear cells (PBMC) stimulated with lipopolysaccharide and in serum were examined by bioassay. We detected IL-6 mRNA expression in all inflamed gingival tissues (17/17) examined and in 2/4 in healthy gingival tissues. IL-6 protein was detected mainly in endothelial cells, fibroblasts, and macrophages but not in the area containing T or B cells in the inflamed gingival tissues, and was not detected at all in healthy gingival tissues. There was no significant difference between the subjects with periodontal disease and those with healthy gingival tissues either in serum IL-6 levels or in the amount of IL-6 produced by PBMC. These results suggest that non-lymphoid cells in inflamed gingival tissue may contribute to the pathogenesis of periodontal disease via IL-6 production, and that the IL-6 produced in gingival tissue may not reflect the IL-6 levels in peripheral blood. 3.

    Web of Science

    researchmap

  • ASSESSMENT OF INTERLEUKIN-6 IN THE PATHOGENESIS OF PERIODONTAL-DISEASE 査読 国際誌

    K TAKAHASHI, S TAKASHIBA, A NAGAI, M TAKIGAWA, F MYOUKAI, H KURIHARA, Y MURAYAMA

    JOURNAL OF PERIODONTOLOGY   65 ( 2 )   147 - 153   1994年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    THIS STUDY WAS PERFORMED TO INVESTIGATE the aspects of interleukin-6 (IL-6) production in both the gingival tissue and the peripheral blood of patients with periodontal disease and of periodontally healthy subjects. In addition, IL-6 expression in human gingival tissues was studied by reverse transcription-polymerase chain reaction analysis and by immunoperoxidase staining with anti-IL-6 monoclonal antibody. The levels of IL-6 in the culture supernatants from peripheral blood mononuclear cells (PBMC) stimulated with lipopolysaccharide and in serum were examined by bioassay. We detected IL-6 mRNA expression in all inflamed gingival tissues (17/17) examined and in 2/4 in healthy gingival tissues. IL-6 protein was detected mainly in endothelial cells, fibroblasts, and macrophages but not in the area containing T or B cells in the inflamed gingival tissues, and was not detected at all in healthy gingival tissues. There was no significant difference between the subjects with periodontal disease and those with healthy gingival tissues either in serum IL-6 levels or in the amount of IL-6 produced by PBMC. These results suggest that non-lymphoid cells in inflamed gingival tissue may contribute to the pathogenesis of periodontal disease via IL-6 production, and that the IL-6 produced in gingival tissue may not reflect the IL-6 levels in peripheral blood. 3.

    Web of Science

    PubMed

    researchmap

  • LEUKOCYTE ADHESION MOLECULES CD11/CD18 AND THEIR ROLE IN PERIODONTAL-DISEASES 査読

    Y MURAYAMA, K KATSURAGI, S TAKASHIBA, H KURIHARA

    MOLECULAR PATHOGENESIS OF PERIODONTAL DISEASE   215 - 233   1994年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:AMER SOC MICROBIOLOGY  

    Web of Science

    researchmap

  • CLONING AND CHARACTERIZATION OF HUMAN TNF-ALPHA PROMOTER REGION 査読

    S TAKASHIBA, L SHAPIRA, S AMAR, TE VANDYKE

    GENE   131 ( 2 )   307 - 308   1993年9月

     詳細を見る

    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    We report the sequence of a 1.2-kb human tumor necrosis factor alpha (TNFalpha) promoter region, which was cloned using PCR. The sequence has several variations from two previous reports and exhibits many potential DNA-binding sites specific to mammalian gene regulatory proteins inducible by lipopolysaccharides.

    DOI: 10.1016/0378-1119(93)90314-S

    Web of Science

    researchmap

  • RAPID FLUOROMETRIC QUANTIFICATION OF MONOCYTE ATTACHMENT IN TISSUE-CULTURE WELLS 査読 国際誌

    L SHAPIRA, S TAKASHIBA, KALMAR, JR, TE VANDYKE, BARAK, V, WA SOSKOLNE

    JOURNAL OF IMMUNOLOGICAL METHODS   165 ( 1 )   93 - 98   1993年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    A simple fluorometric assay that permits rapid quantification of attachment of monocytes or macrophages in tissue culture wells is described. Using 4,6-diamidino-2-phenylindole (DAPI) as a specific fluorochrome marker for DNA, we observed a dose-dependent increase with strong linear correlation in fluorescent emission over a broad range of DNA concentrations. Measurements of the DNA content of the human monocytic cell line THP-1 demonstrated a linear correlation between fluorescence intensity and cell number from 5 x 10(4) to 1 x 10(6) cells, with an estimated average DNA content of 7.5 pg DNA per cell. While untreated THP-1 cells were not detectably adherent, PMA induction for 24 h results in 57-76% adherence to plastic surface. This method was found to be useful for measuring the number of peripheral blood monocytes separated from lymphocytes by attachment. 16 subjects were sampled and the standard deviation of each individual did not exceed 10%. The number of attached cells was between 10-16% of the total mononuclear cells. Fluorescence measurement of DNA with DAPI permits rapid and accurate determination of cell numbers and appears useful in the quantification of adherent populations such as myelocytic cells and cell lines.

    DOI: 10.1016/0022-1759(93)90110-S

    Web of Science

    PubMed

    CiNii Article

    researchmap

  • RAPID FLUOROMETRIC QUANTIFICATION OF MONOCYTE ATTACHMENT IN TISSUE-CULTURE WELLS 査読

    L SHAPIRA, S TAKASHIBA, KALMAR, JR, TE VANDYKE, BARAK, V, WA SOSKOLNE

    JOURNAL OF IMMUNOLOGICAL METHODS   165 ( 1 )   93 - 98   1993年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    A simple fluorometric assay that permits rapid quantification of attachment of monocytes or macrophages in tissue culture wells is described. Using 4,6-diamidino-2-phenylindole (DAPI) as a specific fluorochrome marker for DNA, we observed a dose-dependent increase with strong linear correlation in fluorescent emission over a broad range of DNA concentrations. Measurements of the DNA content of the human monocytic cell line THP-1 demonstrated a linear correlation between fluorescence intensity and cell number from 5 x 10(4) to 1 x 10(6) cells, with an estimated average DNA content of 7.5 pg DNA per cell. While untreated THP-1 cells were not detectably adherent, PMA induction for 24 h results in 57-76% adherence to plastic surface. This method was found to be useful for measuring the number of peripheral blood monocytes separated from lymphocytes by attachment. 16 subjects were sampled and the standard deviation of each individual did not exceed 10%. The number of attached cells was between 10-16% of the total mononuclear cells. Fluorescence measurement of DNA with DAPI permits rapid and accurate determination of cell numbers and appears useful in the quantification of adherent populations such as myelocytic cells and cell lines.

    DOI: 10.1016/0022-1759(93)90110-S

    Web of Science

    researchmap

  • CLONING AND CHARACTERIZATION OF HUMAN TNF-ALPHA PROMOTER REGION 査読 国際誌

    S TAKASHIBA, L SHAPIRA, S AMAR, TE VANDYKE

    GENE   131 ( 2 )   307 - 308   1993年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    We report the sequence of a 1.2-kb human tumor necrosis factor alpha (TNFalpha) promoter region, which was cloned using PCR. The sequence has several variations from two previous reports and exhibits many potential DNA-binding sites specific to mammalian gene regulatory proteins inducible by lipopolysaccharides.

    DOI: 10.1016/0378-1119(93)90314-S

    Web of Science

    PubMed

    CiNii Article

    researchmap

  • INTERLEUKIN-8 IS A MAJOR NEUTROPHIL CHEMOTACTIC FACTOR DERIVED FROM CULTURED HUMAN GINGIVAL FIBROBLASTS STIMULATED WITH INTERLEUKIN-1-BETA OR TUMOR-NECROSIS-FACTOR-ALPHA 査読 国際誌

    S TAKASHIBA, M TAKIGAWA, K TAKAHASHI, F MYOKAI, F NISHIMURA, T CHIHARA, H KURIHARA, Y NOMURA, Y MURAYAMA

    INFECTION AND IMMUNITY   60 ( 12 )   5253 - 5258   1992年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    Inflammatory mediators produced by cells in the gingiva have been implicated in the initiation and progression of periodontal disease, a common infectious disease. In this study, we examined the biological activity of neutrophil chemotactic factors and the kinetics of expression of interleukin-8 (IL-8) mRNA derived from normal gingival fibroblasts in response to inflammatory, mediators in an in vitro model. Gingival fibroblasts stimulated by either recombinant human interleukin-1beta or recombinant human tumor necrosis factor alpha produced neutrophil chemotactic factors after 4 h, whereas expression of cell-derived IL-8 mRNA was detected within 1 h after stimulation. Furthermore, in a neutralization assay, rabbit anti-recombinant human IL-8 antiserum inhibited neutrophil chemotactic activity to basal levels. These results provide evidence that gingival fibroblasts synthesize potent chemotactic factors such as IL-8 in the presence of the inflammatory mediators interleukin-1beta and tumor necrosis factor alpha. The activity of these factors may contribute to neutrophil-mediated processes in the pathogenesis of periodontal disease.

    Web of Science

    PubMed

    researchmap

  • INTERLEUKIN-8 IS A MAJOR NEUTROPHIL CHEMOTACTIC FACTOR DERIVED FROM CULTURED HUMAN GINGIVAL FIBROBLASTS STIMULATED WITH INTERLEUKIN-1-BETA OR TUMOR-NECROSIS-FACTOR-ALPHA 査読

    S TAKASHIBA, M TAKIGAWA, K TAKAHASHI, F MYOKAI, F NISHIMURA, T CHIHARA, H KURIHARA, Y NOMURA, Y MURAYAMA

    INFECTION AND IMMUNITY   60 ( 12 )   5253 - 5258   1992年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    Inflammatory mediators produced by cells in the gingiva have been implicated in the initiation and progression of periodontal disease, a common infectious disease. In this study, we examined the biological activity of neutrophil chemotactic factors and the kinetics of expression of interleukin-8 (IL-8) mRNA derived from normal gingival fibroblasts in response to inflammatory, mediators in an in vitro model. Gingival fibroblasts stimulated by either recombinant human interleukin-1beta or recombinant human tumor necrosis factor alpha produced neutrophil chemotactic factors after 4 h, whereas expression of cell-derived IL-8 mRNA was detected within 1 h after stimulation. Furthermore, in a neutralization assay, rabbit anti-recombinant human IL-8 antiserum inhibited neutrophil chemotactic activity to basal levels. These results provide evidence that gingival fibroblasts synthesize potent chemotactic factors such as IL-8 in the presence of the inflammatory mediators interleukin-1beta and tumor necrosis factor alpha. The activity of these factors may contribute to neutrophil-mediated processes in the pathogenesis of periodontal disease.

    Web of Science

    researchmap

  • A FAMILY STUDY OF A MOTHER AND DAUGHTER WITH INCREASED SUSCEPTIBILITY TO EARLY-ONSET PERIODONTITIS - MICROBIOLOGICAL, IMMUNOLOGICAL, HOST DEFENSIVE, AND GENETIC ANALYSES 査読 国際誌

    F NISHIMURA, A NAGAI, K KURIMOTO, O ISOSHIMA, S TAKASHIBA, M KOBAYASHI, AKUTSU, I, H KURIHARA, Y NOMURA, Y MURAYAMA, H OHTA, K KATO

    JOURNAL OF PERIODONTOLOGY   61 ( 12 )   755 - 765   1990年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    Microbiological, immunological, host-defensive, and genetic analyses were performed on a mother and daughter, both of whom had early-onset periodontitis (rapidly progressive periodontitis in the mother; localized juvenile periodontitis in the daughter). Microscopic examination revealed a greatly elevated percentage of rod-form bacteria in both subjects. Fusobacterium sp. and Porphyromonas gingivalis (formerly Bacteroides gingivalis) were the predominant microorganisms cultured. The humoral immune responses to F. nucleatum, P. gingivalis, and Actinobacillus actinomycetemcomitans were much higher in both subjects than those to any other periodontal bacteria examined. Functional and phenotypic analysis of the peripheral lymphocytes showed no significant abnormalities. However, investigation of neutrophil function showed that the mother had depressed neutrophil chemotaxis and superoxide production. The daughter had depression not only of chemotaxis and superoxide production, but also of neutrophil phagocytosis. Serological typing of HLA antigens revealed the same Class II HLA profile in both subjects. It was concluded that both subjects very probably had an identical condition and that these patients provided a unique model for improving our understanding of the host factors involved in periodontal disease.

    Web of Science

    PubMed

    researchmap

  • A FAMILY STUDY OF A MOTHER AND DAUGHTER WITH INCREASED SUSCEPTIBILITY TO EARLY-ONSET PERIODONTITIS - MICROBIOLOGICAL, IMMUNOLOGICAL, HOST DEFENSIVE, AND GENETIC ANALYSES 査読

    F NISHIMURA, A NAGAI, K KURIMOTO, O ISOSHIMA, S TAKASHIBA, M KOBAYASHI, AKUTSU, I, H KURIHARA, Y NOMURA, Y MURAYAMA, H OHTA, K KATO

    JOURNAL OF PERIODONTOLOGY   61 ( 12 )   755 - 765   1990年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ACAD PERIODONTOLOGY  

    Web of Science

    researchmap

  • POLYMORPHISM OF COMPROMISED HOSTS IN PERIODONTITIS PATIENTS 査読

    S TAKASHIBA, F NISHIMURA, M KOBAYASHI, A NAGAI, AKUTSU, I, K OKAMURA, O ISOSHIMA, H KURIHARA, Y NOMURA, Y MURAYAMA

    RECENT ADVANCES IN CLINICAL PERIODONTOLOGY   790   383 - 386   1988年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:ELSEVIER SCIENCE PUBL B V  

    Web of Science

    researchmap

▼全件表示

書籍等出版物

  • 歯周病と全身の健康

    (NPO) 日本歯周病学会  2016年 

     詳細を見る

  • 歯周病と全身の健康

    (NPO) 日本歯周病学会  2016年 

     詳細を見る

  • 腸内細菌・口腔細菌と全身疾患

    シーエムシー出版  2015年 

     詳細を見る

  • 最新歯科衛生士教本 歯周病学 第2版

    医歯薬出版  2015年 

     詳細を見る

  • 歯周病の検査キット,薬 '15/'16 歯科 疾患名から治療薬と処方例がすぐわかる本.第1版

    クインテッセンス出版  2014年 

     詳細を見る

  • 感染していることがわかった患者への対応(第2部歯科医院に勧める効果的な「院内感染」対策),患者が求める「医療安全」「院内感染」対策

    ヒョーロン・パブリッシャーズ  2014年 

     詳細を見る

  • 臨床歯周病学 第2版

    医歯薬出版株式会社  2013年 

     詳細を見る

  • Kommunikation Der Zellen Illustrierte Beitrage aus der Zahnmedizinischen Forschung und Praxis

    Quintessenz Verlag  2013年 

     詳細を見る

  • イラストで語る歯科医学最前線

    クインテッセンス出版株式会社  2013年 

     詳細を見る

  • At The Forefront Illustrated Topics in Dental Research and Clinical Practice

    Quintessence Publishing Co, Inc  2012年  ( ISBN:9780867155150

     詳細を見る

  • 歯科衛生士のための歯周治療ガイドブック

    医歯薬出版株式界者  2009年 

     詳細を見る

  • 別冊the Quintessence YEAR BOOK 2008 現代の治療指針 歯周治療と全治療分野編

    クインテッセンス出版,東京  2008年 

     詳細を見る

  • 歯科医師・歯科衛生士のための唾液検査ハンドブック

    ヒョーロン・パブリッシャーズ,東京  2008年 

     詳細を見る

  • Preventive Periodontology

    医歯薬出版,東京  2007年 

     詳細を見る

  • 臨床歯周病学

    医歯薬出版,東京  2007年 

     詳細を見る

  • 歯周病と7つの病気,8つのNEWS

    吉江 弘正, 高柴 正悟, 岩本 義博

    永末書店,京都  2007年  ( ISBN:9784816011825

     詳細を見る

    総ページ数:v, 193p, 図版[2]p   記述言語:日本語

    CiNii Books

    researchmap

  • 歯周病の遺伝子治療 : 局所的遺伝子導入による生体反応の制御

    高柴 正悟, 窪木 拓男

    高柴正悟  2006年 

     詳細を見る

    総ページ数:1冊  

    CiNii Books

    researchmap

  • 最新歯科衛生士教本 歯周疾患

    医歯薬出版,東京  2006年 

     詳細を見る

  • 平成15-16年度科学研究費補助金(基盤研究(C)(2)一般)研究成果報告書

    2005年 

     詳細を見る

  • 平成13-15年度科学研究費補助金(基盤研究(C)(2))研究成果報告書

    2004年 

     詳細を見る

  • 平成13-15年度科学研究費補助金 (基盤研究(A)(1))研究成果報告書 (若年者における歯周病の予防システム構築のための総合的研究 研究代表者 昭和大学・歯学部・教授 長谷川紘司)

    2004年 

     詳細を見る

  • 単球系細胞が産生するTNF-αの新規転写因子を制御することによる歯周炎治療

    高柴 正悟

    高柴正悟  2002年 

     詳細を見る

  • 象牙質特異遺伝子を局所に導入することによって歯質保全を図る研究

    高柴 正悟

    高柴正悟  2002年 

     詳細を見る

    総ページ数:1冊  

    CiNii Books

    researchmap

  • ペリオドンタルメディスン

    医歯薬出版株式会社  2001年 

     詳細を見る

  • 先端医療シリーズ・歯科医学2 歯周病 新しい治療を求めて

    寺田国際事務所/先端医療技術研究所  2000年 

     詳細を見る

  • 組織再生性歯周療法 炎症をどうコントロールするか(単著)

    歯周病学最前線 日本歯科評論  2000年 

     詳細を見る

  • Concepts for the biological treatment of periodontal diseases(jointly worked)

    Progress of Periodontal Research and Practice in Asian Pacific Countries  2000年 

     詳細を見る

  • 歯周病の病因 歯周病の遺伝的素因(単著)

    先端医療シリーズ・歯科医学2 歯周病 新しい治療を求めて 寺田国際事務所/先端医療技術研究所  2000年 

     詳細を見る

  • 歯周病学最前線

    日本歯科評論社  2000年 

     詳細を見る

  • Progress of Periodontal Research and Practice in Asian Pacific Countries

    Asian Pacific Society of Periodontology  2000年 

     詳細を見る

  • Concepts for the biological treatment of periodontal diseases(jointly worked)

    Progress of Periodontal Research and Practice in Asian Pacific Countries  2000年 

     詳細を見る

  • 第2編 免疫・遺伝子診断のためのリサーチプロセス,4.白血球からみた感受性診断(共著)

    歯周病診断のストラテジー医歯薬出版  1999年 

     詳細を見る

  • 歯周疾患:晩期発症型歯周炎,早期発症型歯周炎,白血病の歯周炎,妊娠時の歯周炎,糖尿病の歯周炎,口呼吸による歯周炎,薬剤による歯周炎,剥離性歯内炎(共著)

    カラーでみる口腔粘膜疾患の診かた 南江堂  1999年 

     詳細を見る

  • 歯周疾患と遺伝的背景(共著)

    歯周病学 永末書店  1996年 

     詳細を見る

  • Assessment of host defense risk factors for periodontal disease : a case of family members with juvenile periodontitis(jointly worked)

    Risk Factors in Asian Pacific Population  1996年 

     詳細を見る

  • Assessment of host defense risk factors for periodontal disease : a case of family members with juvenile periodontitis(jointly worked)

    Risk Factors in Asian Pacific Population  1996年 

     詳細を見る

  • Leukocyte adhesion molecules CD11/CD18 and their role in periodontal diseases(jointly worked)

    Molecular Pathogenesis of Periodontal Disease ASM Press  1994年 

     詳細を見る

  • Leukocyte adhesion molecules CD11/CD18 and their role in periodontal diseases(jointly worked)

    Molecular Pathogenesis of Periodontal Disease ASM Press  1994年 

     詳細を見る

  • Polymorphism of compromised hosts in periodontitis patients(jointly worked)

    Recent advances in clinical periodontology Elsevier Science Publisher B.V.  1988年 

     詳細を見る

  • Polymorphism of compromised hosts in periodontitis patients(jointly worked)

    Recent advances in clinical periodontology Elsevier Science Publisher B.V.  1988年 

     詳細を見る

▼全件表示

MISC

  • Aggregatibacter actinomycetemcomitansに対し高い血清IgG抗体価反応を示す歯周炎患者の治療経過と病態考察

    岡本 憲太郎, 高知 信介, 小林 寛也, 大森 一弘, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   63 ( 春季特別 )   120 - 120   2021年5月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • ω-3脂肪酸誘導体の抗炎症作用による歯髄保存の試み

    米田 光宏, Zulema Rosalia Arias Martinez, 中村 心, 岡本 憲太郎, 伊東 昌洋, 田村 和也, 井手口 英隆, 大森 一弘, 山城 圭介, 山本 直史, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   154回   140 - 140   2021年5月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

    researchmap

  • X連鎖性低リン血症性くる病を起因とした多発根尖性歯周炎に対し歯内療法を行った症例の病態考察

    佐光 秀文, 大森 一弘, 坂井田 京佑, 亀井 千晶, 小林 寛也, 井手口 英隆, 山本 直史, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   154回   126 - 126   2021年5月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

    researchmap

  • 大豆発酵食品テンペに含まれる抗菌性物質の単離と同定

    伊東 昌洋, 伊東 孝, 中村 心, 青木 秀之, 西岡 功志, 塩川 つぐみ, 多田 宏子, 竹内 祐貴, 武安 伸幸, 山本 直史, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   154回   159 - 159   2021年5月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

    researchmap

  • HMGB1はM1マクロファージの分化を制御して歯周炎の進行に影響を及ぼす

    平井 杏奈, 井手口 英隆, 山城 圭介, Yao Zhang, 青柳 浩明, 山本 直史, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   154回   155 - 155   2021年5月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

    researchmap

  • 歯周病評価における最適検査部位の選定 項目反応理論Graded response modelの応用

    両角 俊哉, 野村 義明, 福田 光男, 花田 信弘, 角田 衣理加, 小林 宏明, 三邉 正人, 中村 利明, 中山 洋平, 西村 英紀, 野口 和行, 沼部 幸博, 小方 頼昌, 齋藤 淳, 佐藤 聡, 関野 愉, 菅野 直之, 菅谷 勉, 鈴木 史彦, 多部田 康一, 高橋 慶壮, 高井 英樹, 高柴 正悟, 梅田 誠, 吉江 弘正, 吉村 篤利, 吉成 伸夫, 中川 種昭

    日本歯周病学会会誌   63 ( 春季特別 )   106 - 106   2021年5月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • 不妊治療中患者に対する血清IgG抗体価検査を用いた歯周病原細菌の感染度調査

    亀井 千晶, 大森 一弘, 佐光 秀文, 坂井田 京佑, 徳善 真砂子, 平井 公人, 小林 寛也, 山本 直史, 滝川 雅之, 三宅 貴仁, 高柴 正悟

    日本歯周病学会会誌   63 ( 春季特別 )   102 - 102   2021年5月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • 歯科保存治療での「保存の可否」とは? 生命を紡ぐ

    高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   154回   40 - 40   2021年5月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

    researchmap

  • 【産婦人科医も知っておきたい歯科の知識】歯周病と不妊

    大森 一弘, 高柴 正悟, 三宅 貴仁

    産科と婦人科   88 ( 4 )   465 - 469   2021年4月

     詳細を見る

    記述言語:日本語   出版者・発行元:(株)診断と治療社  

    歯周病は、Porphyromonas gingivalisに代表される歯周病原細菌が歯周組織に感染して発症する口腔感染症である。近年、歯周病原細菌の感染、そして、感染によって惹起された歯周炎症が不妊環境の構築に関与する可能性が示唆されはじめている。本稿では、歯周病と不妊の関連について文献的に考察するとともに、不妊治療中の重度歯周病患者に専門的治療を行うことによって自然妊娠・正常出産に至った実症例を提示しながら、その関連性を考察する。(著者抄録)

    researchmap

    その他リンク: https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2021&ichushi_jid=J00525&link_issn=&doc_id=20210324150010&doc_link_id=%2Fae4sanke%2F2021%2F008804%2F011%2F0465b0469%26dl%3D3&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fae4sanke%2F2021%2F008804%2F011%2F0465b0469%26dl%3D3&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_4.gif

  • コラーゲン結合型塩基性線維芽細胞増殖因子を用いた水平性骨吸収に対する歯周組織再生療法の開発

    中村 心, 伊東 孝, 岡本 憲太郎, 美間 健彦, 内田 健太郎, 山本 直史, 松下 治, 高柴 正悟

    日本歯科医学会誌   40   78 - 78   2021年3月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本歯科医学会  

    researchmap

  • 臨床推論の教育をどうするか 病態の理解は検査と臨床推論から

    高柴 正悟

    日本口腔診断学会雑誌   34 ( 1 )   52 - 52   2021年2月

     詳細を見る

    記述言語:日本語   出版者・発行元:(一社)日本口腔診断学会  

    researchmap

  • 歯内療法の薬物的な新規治療

    高柴 正悟

    日本歯内療法学会雑誌   42 ( 1 )   1 - 4   2021年1月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本歯内療法学会  

    炎症の場においては、組織の炎症を調整するメディエーターが急性期に作用して炎症を解消し、炎症性に破壊された組織を結果的に再生へ向かわせる。この作用を活用した炎症性疾患治療の、歯内療法への応用を紹介した。歯髄や根尖歯周組織の炎症を調節し、組織の修復・再生に向かわせる生体反応調節性の歯内療法薬剤の研究開発が期待されている。

    researchmap

    その他リンク: https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2021&ichushi_jid=J03868&link_issn=&doc_id=20210209200001&doc_link_id=10.20817%2Fjeajournal.42.1_1&url=https%3A%2F%2Fdoi.org%2F10.20817%2Fjeajournal.42.1_1&type=J-STAGE&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00007_3.gif

  • 【ペリオと○○-最新歯周治療トピックス集-】(Topic 5)ペリオとPISA 歯周組織の炎症面積で歯周病の重症度を表現しよう!

    高柴 正悟

    デンタルハイジーン   41 ( 1 )   40 - 42   2021年1月

     詳細を見る

    記述言語:日本語   出版者・発行元:医歯薬出版(株)  

    researchmap

  • 子宮内膜症の治療と妊娠を契機に進行したと疑われる慢性歯周炎患者の病態考察と治療経過

    坂井田 京佑, 大森 一弘, 小林 寛也, 山本 直史, 高柴 正悟

    岡山歯学会雑誌   39 ( 2 )   36 - 37   2020年12月

     詳細を見る

    記述言語:日本語   出版者・発行元:岡山歯学会  

    researchmap

  • 侵襲性歯周炎の血液診断バイオマーカーとしての細胞外小胞由来マイクロRNAの探索

    河本 美奈, 山本 直史, 河村 麻理, 森 彩乃, 山城 圭介, 大森 一弘, 小野 喜章, 江口 傑徳, 十川 千春, 高柴 正悟

    岡山歯学会雑誌   39 ( 2 )   35 - 36   2020年12月

     詳細を見る

    記述言語:日本語   出版者・発行元:岡山歯学会  

    researchmap

  • 臨床推論の教育をどうするか 病態の理解は検査と臨床推論から

    高柴 正悟

    日本口腔内科学会雑誌   26 ( 2 )   100 - 100   2020年12月

     詳細を見る

    記述言語:日本語   出版者・発行元:(一社)日本口腔内科学会  

    researchmap

  • 真菌代謝産物(+)-terreinがマウス骨粗鬆症モデルにおける骨代謝に及ぼす影響

    坂井田 京佑, 大森 一弘, 中川 沙紀, 佐光 秀文, 亀井 千晶, 山本 総司, 小林 寛也, 山城 圭介, 山本 直史, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   153回   36 - 36   2020年11月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

    researchmap

  • High Mobility Group Box 1(HMGB1)はマクロファージからのCCL2分泌を制御して抜歯窩の歯周組織再生を促進する

    井手口 英隆, 平井 杏奈, 山城 圭介, 京嶌 里沙, 青柳 浩明, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   62 ( 秋季特別 )   121 - 121   2020年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • 急性期脳卒中患者における喀痰内の多剤耐性菌検出とその関連因子(横断研究)

    井上 裕貴, 松永 一幸, 坪井 綾香, 山城 圭介, 高柴 正悟

    日本歯周病学会会誌   62 ( 秋季特別 )   125 - 125   2020年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • 歯周組織の炎症と不妊の関連性を示唆する、ある侵襲性歯周炎患者の病態生理

    大森 一弘, 河野 隆幸, 小林 寛也, 新井 英雄, 山本 直史, 高柴 正悟

    日本歯科保存学雑誌   63 ( 5 )   451 - 460   2020年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

    緒言:歯周病原細菌の感染と歯周組織の炎症が,妊娠に影響を与える可能性が報告されている.今回,不妊治療の経過が思わしくない侵襲性歯周炎患者に感染源除去の観点から専門的歯周治療を行い,自然妊娠から正常出産にいたった症例の経過をふまえ病態を考察する.症例:33歳,女性,既婚(不妊治療中).2016年9月,26の動揺および同部の自発痛を自覚し,かかりつけ歯科医院を受診した.同院でエックス線検査を受けて,重度の歯槽骨吸収があると説明された.早期の専門的歯周治療を勧められ,当科を紹介された.既往歴の特記事項はなく,不妊検査においても患者本人および夫ともに異常所見はなかった.歯周組織検査において,probing pocket depthが4mm以上の部位の割合は49.5%,bleeding on probingは47.9%,plaque control recordは3.1%,歯周炎症表面積(PISA)は2,392mm2であった.エックス線検査所見では,主訴部の26部を中心に根尖に及ぶ骨吸収像が多数存在した.歯周病原細菌に対する血清抗体価検査および歯周ポケット内細菌DNA検査ともに,Porphyromonas gingivalisの感染が強く疑われた.診断は広汎型侵襲性歯周炎(ステージIV,グレードC),二次性咬合性外傷とした.治療方針として,患者の妊娠希望に配慮して,できるかぎり早期(1年以内)の歯周環境の改善を目指すこととした.また,歯周外科治療が終了するまでの不妊治療を含めた妊娠活動を控える必要性について説明し,同意を得た.治療計画は,(1)歯周基本治療(患者教育,抜歯,局所抗菌療法を併用したスケーリング・ルートプレーニング,暫間固定),(2)歯周組織再生療法,(3)口腔機能回復治療,(4)歯周病安定期治療(SPT)とした.治療経過として,歯周治療に対する宿主反応性は非常に良く,炎症改善と歯槽骨の再生を確認した(歯周外科治療後PISA:43mm2).口腔機能回復治療中に自然妊娠し,35歳時に男児を正常出産(経腟分娩,3,240g,出産週数:38週+5日)した.考察および結論:重度のP. gingivalis感染および歯周炎症を伴う侵襲性歯周炎の罹患が,妊娠成立に影響を及ぼす可能性が示唆された.本症例のように不妊治療の経過が思わしくない場合には,歯周組織を含めた口腔状態を一度精査することが望まれる.(著者抄録)

    researchmap

    その他リンク: https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2020&ichushi_jid=J01092&link_issn=&doc_id=20201110310013&doc_link_id=%2Fdo1conde%2F2020%2F006305%2F013%2F0451-0460%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fdo1conde%2F2020%2F006305%2F013%2F0451-0460%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

  • 急性期脳卒中患者における喀痰内の多剤耐性菌検出とその関連因子(横断研究)

    井上 裕貴, 松永 一幸, 山城 圭介, 高柴 正悟

    日本未病学会学術総会抄録集   27回   107 - 107   2020年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:(一社)日本未病学会  

    researchmap

  • 歯の病的移動と歯周-歯内病変を併発した侵襲性歯周炎患者に対する歯周組織再生療法

    本行 令奈, 井手口 英隆, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   62 ( 秋季特別 )   142 - 142   2020年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • 24年間の治療効果に関係なく高い抗P.gingivalis IgG抗体価を示す限局性侵襲性歯周炎患者

    峯柴 淳二, 峯柴 史, 岩本 義博, 高柴 正悟

    日本歯周病学会会誌   62 ( 秋季特別 )   133 - 133   2020年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • 次の時代を見据えた戦略的未病対策とは 歯科疾患の未病対策は全身の未病対策に繋がる

    高柴 正悟

    日本未病学会学術総会抄録集   27回   71 - 71   2020年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:(一社)日本未病学会  

    researchmap

  • ハンチントン舞踏病の広汎型慢性歯周炎患者への歯周病治療で感じた歯科衛生士の役割

    佐藤 由実, 伊藤 実有, 北村 彰子, よし田 亜紀, 苅田 奈生子, 磯島 大地, 伊東 昌洋, 長島 義之, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   62 ( 秋季特別 )   152 - 152   2020年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • 新しい日本での歯周病治療と歯周病専門医の展開

    高柴 正悟

    日本歯周病学会会誌   62 ( 3 )   129 - 135   2020年9月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    超高齢社会の日本において医療界、特に歯科医療界がどこに向かうのか考察した。大学を含む病院が主な勤務先である医師とは異なり、歯科医師はほとんどが歯科診療所に勤務している。また、厚労省の統計によると歯科医師は医師よりも平均年齢が7歳若く、これは高齢者の割合が増加している患者への対応方法に若干の違いをもたらしている可能性がある。一方で医師と歯科医師の分布の地域差が顕著であり、来たるべき歯科医師の高齢化についても配慮が必要となる。こうした社会の変化の中で、日本歯周病学会の歯周病専門医は厚生労働省の「医療に関する広告が可能となった医師等の専門性に関する資格名」として周知されている。今後は、(一社)日本歯科専門医機構の承認も得ることには違いない。また、高度で広範囲な歯周病専門医による治療を日本国内の各地に均平化することも必要である。

    researchmap

    その他リンク: https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2020&ichushi_jid=J01095&link_issn=&doc_id=20201009370001&doc_link_id=130007919193&url=https%3A%2F%2Fci.nii.ac.jp%2Fnaid%2F130007919193&type=CiNii&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00003_1.gif

  • インテグリンα3の選択的阻害による微小環境の構築と歯槽骨再生

    森 彩乃, 山本 直史, 河村 麻理, 井手口 英隆, 青柳 浩明, 中村 心, 岡本 憲太郎, 平井 杏奈, 山城 圭介, 大森 一弘, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   152回   142 - 142   2020年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

    researchmap

  • 歯髄壊死した根未完成下顎第二小臼歯に対してrevascularizationを行った症例

    佐光 秀文, 高柴 正悟

    日本歯内療法学会学術大会プログラム・抄録集   41回   91 - 91   2020年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本歯内療法学会  

    researchmap

  • 血糖管理不良の2型糖尿病に罹患する重度慢性歯周炎患者への歯周治療

    山城 圭介, 新井 英雄, アリアス・スレマ, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   152回   55 - 55   2020年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

    researchmap

  • High Mobility Group Box 1が抜歯窩治癒過程の間葉系幹細胞の遊走に及ぼす影響

    京嶌 里紗, 井手口 英隆, 山城 圭介, 平井 杏奈, 青柳 浩明, 山本 直史, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   152回   141 - 141   2020年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

    researchmap

  • 歯周病重症度マーカーとしての洗口液・唾液・歯肉溝滲出液中の短鎖脂肪酸

    畑中 加珠, 川瀬 貴博, 白波瀬 泰史, 河野 麻理, 吉田 敏之, 塚原 隆充, 落合 邦康, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   62 ( 春季特別 )   145 - 145   2020年5月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • 真菌二次代謝産物(+)-terreinはTNF-αの発現を抑制し歯周炎マウスモデルにおける歯槽骨吸収を抑制する

    佐光 秀文, 大森 一弘, 中川 沙紀, 亀井 千晶, 坂井田 京佑, 山本 総司, 井手口 英隆, 小林 寛也, 山城 圭介, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   62 ( 春季特別 )   141 - 141   2020年5月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • 歯周組織再生療法の違いが歯肉縁下細菌叢に及ぼす影響 侵襲性歯周炎患者の一症例

    大森 一弘, 河野 隆幸, 新井 英雄, 小林 寛也, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   62 ( 春季特別 )   165 - 165   2020年5月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • 急性期脳卒中患者における入院時評価項目と喀痰内の薬剤耐性菌検出状況に関する調査

    井上 裕貴, 松永 一幸, 山城 圭介, 高柴 正悟

    日本口腔診断学会雑誌   33 ( 1 )   119 - 119   2020年2月

     詳細を見る

    記述言語:日本語   出版者・発行元:(一社)日本口腔診断学会  

    researchmap

  • 歯科用接着性レジン系セメントを用いた補綴物装着によるアレルギー発症症例と検査の考察

    山城 圭介, 北川 雅恵, 岡 広子, 高柴 正悟

    日本口腔診断学会雑誌   33 ( 1 )   88 - 88   2020年2月

     詳細を見る

    記述言語:日本語   出版者・発行元:(一社)日本口腔診断学会  

    researchmap

  • 細菌性コラゲナーゼのコラーゲン・アンカーの構造活性相関と歯周病治療への応用

    松下 治, 美間 健彦, 後藤 和義, 山本 由弥子, Caviness Perry, Sakon Joshua, 内田 健太郎, 中村 心, 岡本 健太郎, 高柴 正悟

    日本細菌学雑誌   75 ( 1 )   138 - 138   2020年1月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本細菌学会  

    researchmap

  • 真菌二次代謝産物terreinはマウス歯周病モデルにおける歯槽骨吸収を抑制する

    佐光 秀文, 大森 一弘, 中川 沙紀, 坂井田 京佑, 山本 総司, 青柳 浩明, 小林 寛也, 大野 充昭, 平井 公人, 山城 圭介, 山本 直史, 高柴 正悟

    有病者歯科医療   28 ( 6 )   430 - 431   2019年12月

     詳細を見る

    記述言語:日本語   出版者・発行元:(一社)日本有病者歯科医療学会  

    researchmap

  • フッ素イオンによるCCNファミリー遺伝子の制御

    水川 朋美, 西田 崇, 明石 翔, 堀 彩花, 高柴 正悟, 上岡 寛, 滝川 正春, 久保田 聡

    岡山歯学会雑誌   38 ( 2 )   85 - 85   2019年12月

     詳細を見る

    記述言語:日本語   出版者・発行元:岡山歯学会  

    researchmap

  • 急性期脳卒中患者における入院時評価項目と喀痰内の薬剤耐性菌検出状況に関する調査

    井上 裕貴, 松永 一幸, 山城 圭介, 高柴 正悟

    日本口腔内科学会雑誌   25 ( 2 )   109 - 109   2019年12月

     詳細を見る

    記述言語:日本語   出版者・発行元:(一社)日本口腔内科学会  

    researchmap

  • 歯科用接着性レジン系セメントを用いた補綴物装着によるアレルギー発症症例と検査の考察

    山城 圭介, 北川 雅恵, 岡 広子, 高柴 正悟

    日本口腔内科学会雑誌   25 ( 2 )   78 - 78   2019年12月

     詳細を見る

    記述言語:日本語   出版者・発行元:(一社)日本口腔内科学会  

    researchmap

  • 多施設後ろ向き観察研究による臨床指標としての歯周炎症表面積の基準値

    井上 裕貴, 畑中 加珠, 山本 直史, 平田 貴久, 三辺 正人, 山本 龍生, 内藤 徹, 山本 松男, 佐藤 秀一, 石幡 浩志, 稲垣 幸司, 三谷 章雄, 中島 啓介, 漆原 譲治, 高柴 正悟

    日本歯周病学会会誌   61 ( 4 )   159 - 167   2019年12月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    歯周炎症表面積(periodontal inflamed surface area:PISA)は,歯周組織の炎症部の面積を表す新たな歯周病の臨床指標である。従来伝わりづらかった歯周病の炎症程度を歯科以外の医療従事者が理解する上で,有用な指標であると考えられる。しかし,歯周病の程度や治療によるPISAの基準は未だ不明である。そこで,本研究は,日本歯周病学会が設ける歯周病専門医・認定医の電子申請書類のデータから各治療フェーズにおけるPISAの値を調べ,歯周病治療に伴う炎症度の基準値を提案することを目的とした。8施設で取得した113症例を用いて,Nesseらの方法によって歯周ポケット深さとプロービング時の出血からPISAを算出した。その結果,PISAの中央値は,初診時1,271.4mm2,歯周基本治療終了時211.8mm2,SPT移行時52.1mm2,そして最新SPT時30.0mm2であった。また,PISAはBOPと高い相関を示し(p<0.001),BOPよりも鋭敏に治療効果を反映した。以上から,中等度以上の歯周炎においてPISAを用いると,初診時は表面積約1,500mm2の歯周組織の炎症がSPT時は100mm2未満(初診時の約7%)に減少することが明らかになった。今後,更なるデータの蓄積および詳細な分析を行いながらPISAの使用を普及させると,PISAは医科歯科連携の際に歯周病炎症を伝える指標になり得ると考える。(著者抄録)

    researchmap

    その他リンク: https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2019&ichushi_jid=J01095&link_issn=&doc_id=20200128510001&doc_link_id=10.2329%2Fperio.61.159&url=https%3A%2F%2Fdoi.org%2F10.2329%2Fperio.61.159&type=J-STAGE&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00007_3.gif

  • 歯周組織の炎症と不妊との関連性を示唆する侵襲性歯周炎症例の病態生理 岡山大学病院・侵襲性歯周炎センターでの取り組み

    亀井 千晶, 大森 一弘, 小林 寛也, 山本 直史, 高柴 正悟

    岡山歯学会雑誌   38 ( 2 )   91 - 92   2019年12月

     詳細を見る

    記述言語:日本語   出版者・発行元:岡山歯学会  

    researchmap

  • 基幹病院⇔診療所間の医療情報連携の現実と理想

    高柴 正悟, 岡山大学病院歯周科:医療情報部専門委員会

    医療情報学連合大会論文集   39回   86 - 87   2019年11月

     詳細を見る

    記述言語:日本語   出版者・発行元:(一社)日本医療情報学会  

    researchmap

  • 広汎型中等度慢性歯周炎患者に対して衛生管理しやすい口腔内環境を確立した症例

    田村 仁美, 清水 明美, 伊東 孝, 伊東 有希, 河野 隆幸, 高柴 正悟

    日本歯周病学会会誌   61 ( 秋季特別 )   168 - 168   2019年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • 急性期脳卒中患者における喀痰内の薬剤耐性菌検出状況と関与する背景因子

    井上 裕貴, 松永 一幸, 山城 圭介, 高柴 正悟

    日本未病システム学会学術総会抄録集   26回   131 - 131   2019年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:(一社)日本未病学会  

    researchmap

  • 真菌二次代謝産物terreinはマウス骨粗鬆症モデルにおいて大腿骨吸収を抑制する

    坂井田 京佑, 大森 一弘, 中川 沙紀, 佐光 秀文, 山本 総司, 小林 寛也, 平井 公人, 山城 圭介, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   61 ( 秋季特別 )   146 - 146   2019年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • High Mobility Group Box 1(HMGB1)は間葉系幹細胞の遊走を介して抜歯窩の創傷治癒を促進する

    平井 杏奈, 井手口 英隆, 山城 圭介, 青柳 浩明, 鈴木 里紗, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   61 ( 秋季特別 )   137 - 137   2019年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • 二次性咬合性外傷を伴う重度慢性歯周炎患者への歯周組織再生治療の成功要因

    山本 直史, 新井 英雄, 高柴 正悟

    日本歯周病学会会誌   61 ( 秋季特別 )   165 - 165   2019年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • 広汎型中等度慢性歯周炎患者の24年経過からみたSPTの重要性

    福家 教子, 佐藤 佐智子, 新井 英雄, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   61 ( 秋季特別 )   156 - 156   2019年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • Porphyromonas gingivalisのPGN_0297の機能解析

    小野 晋太郎, 中山 真彰, 大原 直也, 高柴 正悟

    日本歯周病学会会誌   61 ( 秋季特別 )   124 - 124   2019年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • 【糖尿病専門医として知っておきたい歯周炎のこと】歯周病の新しい捉え方

    高柴 正悟

    月刊糖尿病   11 ( 4 )   46 - 55   2019年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:(株)医学出版  

    researchmap

  • 周期的伸展刺激と静水圧刺激に対するヒト歯根膜細胞の形態と配向の変化

    藤田 彩乃, 高柴 正悟

    岡山歯学会雑誌   38 ( 1 )   1 - 12   2019年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:岡山歯学会  

    ヒト歯根膜細胞の伸展刺激による配向性変化と、静水圧刺激による細胞形態変化について、in vitro機械刺激負荷制御システムを用いて検討した。結果、伸展刺激開始後6時間で伸展方向に対して60〜75°に配向し、静水圧刺激20MPa以上で細胞形態が変化し、40MPa以上で核形態も変化した。

    researchmap

  • 歯周病原細菌を原因とするヒトと伴侶動物の犬における人獣共通感染症検査に関する研究

    田井 真砂子, 高柴 正悟

    岡山歯学会雑誌   38 ( 1 )   1 - 19   2019年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:岡山歯学会  

    ヒトと犬における歯周病原細菌の交差感染を調べるための検査方法として、ヒトで確立されているリアルタイムPCR法による細菌DNA検査およびELISA法による血清IgG抗体検査を用い、代表的な歯周病原細菌であるP.gingivalisとP.gulaeを対象とした検査を確立することを目的に研究を行った。リアルタイムPCRで推奨される増幅DNA長150bp以下のプライマーを作製するとともに、ELISAに用いる両細菌の超音波破砕抽出抗原を作製し、犬に対する有用性について検討した。結果、作製したプライマー・抗原は犬の歯周病検査にも有用と考えられた。

    researchmap

  • 歯科関連行動とIgG抗体価で示す歯周病原細菌の感染度との関連の横断研究

    坪井 綾香, 高柴 正悟

    岡山歯学会雑誌   38 ( 1 )   1 - 10   2019年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:岡山歯学会  

    2008〜2015年に全国の日本歯周病学会会員(歯科医院)で市販の自己採血キットを用いて採取された血液から得られたIgG抗体価と、採血時の問診票に記載された歯科関連行動との関連性について検討した。対象は、全9286データ中、40歳未満と重複分を除外した5602データとした。検討の結果、年1回以上の歯科健診および歯石除去は歯周病原菌感染度と関連していることが明らかになり、この関連は特に女性と65歳未満者において顕著であった。

    researchmap

  • コラーゲン結合型塩基性線維芽細胞増殖因子とコラーゲン基剤を用いた複合剤は水平性骨欠損における歯周組織再生を促進する

    中村 心, 高柴 正悟

    岡山歯学会雑誌   38 ( 1 )   1 - 14   2019年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:岡山歯学会  

    コラーゲン結合型bFGF(CB-bFGF)は整形外科領域において骨形成促進作用が報告されており、マウスの大腿骨骨折モデルに対してCB-bFGFにコラーゲン基剤を併用すると、bFGF単独に比べて骨折部の骨形成が有意に促進されたと報告されている。そこで、CB-bFGFとコラーゲン基剤を用いた複合剤を歯周組織再生療法に応用することを考えた。しかし、応用するには口腔の解剖学的特徴すなわち、創部が開放創となりやすく、材料が流出しやすいという点を考慮する必要がある。また、整形外科領域の報告にある長管骨は中胚葉由来であるのに対し、歯周組織は外胚歯由来であり、発生学的に異なる背景をもつ。これらのことを踏まえて複合剤の効果をin vitroとin vivoで検討した結果、bFGF単独に比べて有意に歯周組織再生を促進することが確認された。

    researchmap

  • Rhizopus stolonifer NBRC 30816を用いて作製した大豆発酵食品テンペに含まれる抗菌性物質の単離と同定

    伊東 昌洋, 高柴 正悟

    岡山歯学会雑誌   38 ( 1 )   1 - 16   2019年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:岡山歯学会  

    テンペは、インドネシア原産のRhizopus属によって調製された伝統的な非塩漬け大豆発酵食品であり、発酵には、R.stolonifer、R.oligosporus、R.oryzaeがそれぞれ単独あるいは混合して使用される。テンペはグラム陽性菌に対して抗菌活性を示すと報告されていることから、著者等はテンペが口腔内細菌に対しても抗菌性をもつという仮説を立て、検証を行った。方法は、R.stolonifer、R.oligosporus、R.oryzaeそれぞれを用いて調製した3種のテンペについて、口腔内グラム陽性球菌に対する抗菌性を調べるとともに、各テンペに含まれる抗菌性物質の単離・同定を試みた。結果、3種のうちR.stoloniferで調製したテンペは抗菌性が最も強く、同テンペの粗抽出液は1mg/mL以上の濃度でS.mutansに対して抗菌性を示した。同テンペ中に含まれる抗菌性物質はリノール酸であると考えられた。

    researchmap

  • 歯周組織の炎症と不妊の関連性を示唆するある侵襲性歯周炎患者の病態生理

    大森 一弘, 河野 隆幸, 小林 寛也, 新井 英雄, 山本 直史, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   150回   58 - 58   2019年5月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

    researchmap

  • 子宮全摘出・卵巣片側摘出直後から急性化した重度慢性歯周炎症例の治療と病態考察

    坂井田 京佑, 大森 一弘, 佐光 秀文, 小林 寛也, 高知 信介, 河野 隆幸, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   61 ( 春季特別 )   157 - 157   2019年5月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • 徹底した感染管理が垂直性骨欠損を改善する要因であった重度慢性歯周炎症例

    大久保 圭祐, 高知 信介, 本郷 昌一, 河野 隆幸, 大森 一弘, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   61 ( 春季特別 )   152 - 152   2019年5月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • 周期性好中球減少症を有する母娘に認められた重度歯周炎の症例

    二宮 雅美, 坂本 英次郎, 成石 浩司, 生田 貴久, 高木 亮輔, 畑中 加珠, 岡本 憲太郎, 小野 晋太郎, 高柴 正悟, 湯本 浩通

    日本歯周病学会会誌   61 ( 春季特別 )   164 - 164   2019年5月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • コラーゲン結合型塩基性線維芽細胞成長因子はコラーゲン基剤からの徐放によって歯周組織再生を促進する

    岡本 憲太郎, 中村 心, 伊東 孝, Siddiqui Yasir Dilshad, 美間 健彦, 内田 健太郎, 大森 一弘, 山本 直史, 松下 治, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   150回   113 - 113   2019年5月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

    researchmap

  • 細菌性コラゲナーゼのコラーゲン・アンカーと歯周組織再生への応用(Collagen anchors of bacterial collagenases and their application to periodontal tissue regeneration)

    松下 治, 美間 健彦, 後藤 和義, 山本 由弥子, Perry Caviness, Sakon Joshua, 小出 隆規, 内田 健太郎, 中村 心, 高柴 正悟

    日本細菌学雑誌   74 ( 1 )   84 - 84   2019年3月

     詳細を見る

    記述言語:英語   出版者・発行元:日本細菌学会  

    researchmap

  • 歯科から医療界へ発信する「口腔の感染・炎症・機能」に基づく歯周病の包括的臨床検査の確立

    高柴 正悟, 栗原 英見, 山崎 和久, 西村 英紀, 三辺 正人, 和泉 雄一

    日本歯科医学会誌   38   47 - 51   2019年3月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本歯科医学会  

    医科歯科連携に深く関連する歯周病関連部分を、医療全般から理解される検査として再構築することによって、プロジェクト研究テーマB「歯科診療における臨床検査の新規開発」の一部へ対応させる。これによって、21世紀の医療において、歯科医療のみならず医療全般に変革をもたらす検査法と診断体系の開発に繋がる。ペリオドンタルメディシンの考え方が普及して、口腔と全身の疾患との関連(平成28年3月に日本歯周病学会が『歯周病と全身の健康』を刊行)の中から特に糖尿病治療体系に歯周病治療が取り入れられ、さらには抗菌剤のポケット内への投与が保険収載されるようにもなった。こうした展開を歯周病が関連する他の疾患へも広げることが、歯科医療のみならず医療全般の充実をもたらす。そこで、歯周病の発症と進行、口腔機能の喪失、全身への影響という観点から、(1)感染、(2)炎症、(3)咬合力・咀嚼率、といった3つの因子を包括的に把握する。これらの3分野の組み合せで歯周病の病状を捉えるように解析方法を開発するとともに、歯周病が関連する疾患への影響度の指標となるように解析方法を検討した。(著者抄録)

    researchmap

  • 真菌二次代謝産物terreinはマウス歯周病モデルにおける歯槽骨吸収を抑制する

    佐光 秀文, 大森 一弘, 中川 沙紀, 坂井田 京佑, 山本 総司, 青柳 浩明, 小林 寛也, 大野 充昭, 平井 公人, 山城 圭介, 山本 直史, 高柴 正悟

    歯科薬物療法   38 ( 2 )   128 - 128   2019年3月

     詳細を見る

    記述言語:日本語   出版者・発行元:(一社)日本歯科薬物療法学会  

    researchmap

  • 専門外来最前線 侵襲性歯周炎に対する専門外来での対応 岡山大学病院・侵襲性歯周炎センターの取り組み

    大森 一弘, 高柴 正悟

    日本歯科評論   79 ( 3 )   141 - 147   2019年3月

     詳細を見る

    記述言語:日本語   出版者・発行元:(株)ヒョーロン・パブリッシャーズ  

    researchmap

  • 【歯周病と糖尿病・代謝疾患】歯周病と菌血症・感染症

    高柴 正悟

    内分泌・糖尿病・代謝内科   48 ( 2 )   108 - 113   2019年2月

     詳細を見る

    記述言語:日本語   出版者・発行元:(有)科学評論社  

    researchmap

  • 歯周病治療介入は誤嚥性肺炎を抑制できるのか? 歯周炎と誤嚥性肺炎の関係

    高柴 正悟

    日本臨床歯周病学会会誌   36 ( 2 )   40 - 42   2019年2月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本臨床歯周病学会  

    researchmap

  • 当院における成人先天性心疾患患者の口腔状態の現況(Current Oral Condition of Patients with Adult Congenital Heart Disease in ACHD Center/Okayama University Hospital)

    大森 一弘, 杜 徳尚, 高知 信介, 山本 直史, 赤木 禎治, 伊藤 浩, 高柴 正悟

    日本成人先天性心疾患学会雑誌   8 ( 1 )   142 - 142   2019年1月

     詳細を見る

    記述言語:英語   出版者・発行元:日本成人先天性心疾患学会  

    researchmap

  • Porphyromonas gingivalisのジンジパインの機能発現におけるPGN_0297の役割

    小野 晋太郎, 高柴 正悟, 大原 直也

    岡山歯学会雑誌   37 ( 2 )   81 - 82   2018年12月

     詳細を見る

    記述言語:日本語   出版者・発行元:岡山歯学会  

    researchmap

  • Porphyromonas gingivalis感染合併非アルコール性脂肪肝疾患に対する病態把握と治療の目安となる歯周組織検査所見の探索 多施設共同前向き観察研究

    鎌田 要平, 結束 貴臣, 清水 智子, 佐藤 五月, 青山 典生, 高柴 正悟, 中島 淳, 三辺 正人

    神奈川歯学   53 ( 抄録集 )   53 - 53   2018年12月

     詳細を見る

    記述言語:日本語   出版者・発行元:神奈川歯科大学学会  

    researchmap

  • 岡山大学病院・糖尿病教育入院患者を対象とした医科歯科連携システムの概況

    清水 由梨香, 大森 一弘, 利根 淳仁, 高知 信介, 山本 直史, 和田 淳, 高柴 正悟

    岡山歯学会雑誌   37 ( 2 )   87 - 87   2018年12月

     詳細を見る

    記述言語:日本語   出版者・発行元:岡山歯学会  

    researchmap

  • 歯科用ユニット給水管路汚染対策に向けた自動注水型試験機の有効性評価

    岡本 憲太郎, 大久保 圭祐, 伊東 孝, 伊東 昌洋, 田井 真沙子, 中村 心, 山口 唯菜, 塩田 康祥, 河田 有祐, 大森 一弘, 山本 直史, 高柴 正悟

    岡山歯学会雑誌   37 ( 2 )   83 - 84   2018年12月

     詳細を見る

    記述言語:日本語   出版者・発行元:岡山歯学会  

    researchmap

  • 医療連携に必要な医療情報と医療連携レベル・患者個人レベルでのICT格差

    高柴 正悟, 医療情報部専門委員会

    医療情報学連合大会論文集   38回   158 - 159   2018年11月

     詳細を見る

    記述言語:日本語   出版者・発行元:(一社)日本医療情報学会  

    researchmap

  • 歯周炎罹患犬における歯周ポケット表面積推算法の確立と臨床的意義

    田村 和也, 田井 真砂子, 永原 未悠, 永原 美治, 高柴 正悟

    動物臨床医学会年次大会プロシーディング   39回 ( 2 )   131 - 132   2018年11月

     詳細を見る

    記述言語:日本語   出版者・発行元:動物臨床医学会  

    researchmap

  • コラーゲン結合型塩基性線維芽細胞成長因子とコラーゲン基剤を用いた複合剤の歯周組織再生への応用

    中村 心, 伊東 孝, 松下 治, 岡本 憲太郎, 美間 健彦, 内田 健太郎, Siddiqui Yasir Dilshad, 伊東 昌洋, 田井 真砂子, 大久保 圭祐, 山城 圭介, 大森 一弘, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   60 ( 秋季特別 )   115 - 115   2018年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • 歯周病の炎症度を示す臨床的検査基準値の検討

    井上 裕貴, 畑中 加珠, 大森 一弘, 山本 直史, 三辺 正人, 高柴 正悟, 平田 貴久, 山本 龍生, 内藤 徹, 山本 松男, 佐藤 秀一, 石幡 浩志, 稲垣 幸司, 三谷 章雄, 中島 啓介, 漆原 譲治

    日本未病システム学会学術総会抄録集   25回   108 - 108   2018年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:(一社)日本未病学会  

    researchmap

  • Porphyromonas gingivalis感染合併非アルコール性脂肪肝疾患に対する病態把握と治療の目安となる歯周組織検査所見の探索 多施設共同前向き観察研究

    鎌田 要平, 結束 貴臣, 清水 智子, 佐藤 五月, 青山 典生, 小林 貴, 米田 正人, 畑中 加珠, 高柴 正悟, 岩崎 知之, 栗橋 健夫, 児玉 利朗, 田村 利之, 井野 智, 中島 淳, 三辺 正人

    日本歯周病学会会誌   60 ( 秋季特別 )   115 - 115   2018年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • 真菌二次代謝産物(+)-terreinはマウス実験的歯周炎モデルにおける歯槽骨吸収を抑制する

    佐光 秀文, 大森 一弘, 中川 沙紀, 坂井田 京佑, 山本 総司, 青柳 浩明, 小林 寛也, 山城 圭介, 山本 直史, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   149回   150 - 150   2018年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

    researchmap

  • 歯周病原細菌によるヒトと伴侶動物イヌとの人獣共通感染症検査の研究

    田井 真砂子, 伊東 孝, 平山 晴子, 矢田 範夫, 小川 寛人, 田村 和也, 伊東 有希, 大久保 圭祐, 伊東 昌洋, 中村 心, 岡本 憲太郎, 平井 公人, 山城 圭介, 大森 一弘, 山本 直史, 樅木 勝巳, 高柴 正悟

    日本歯周病学会会誌   60 ( 秋季特別 )   135 - 135   2018年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • 侵襲性歯周炎患者の血漿エクソソーム由来microRNAの発現解析

    高木 美奈, 山本 直史, 河村 麻理, 高知 信介, 山城 圭介, 大森 一弘, 江口 傑徳, 十川 千春, 高柴 正悟

    日本歯周病学会会誌   60 ( 秋季特別 )   134 - 134   2018年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • 侵襲性歯周炎患者の専門外来部門連携による包括的な治療と病態解析

    高知 信介, 久保 克行, 山本 直史, 高柴 正悟

    日本歯周病学会会誌   60 ( 秋季特別 )   148 - 148   2018年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • 歯周病患者における機能指標としての咀嚼機能検査の有用性について

    宮沢 春菜, 中島 貴子, 松川 由実, 清水 伸太郎, 古市 保志, 根本 英二, 高井 英樹, 中山 洋平, 小方 頼昌, 岩崎 拓也, 石原 裕一, 大井 麻子, 齋藤 淳, 藤原 千春, 村上 伸也, 畑中 加珠, 高柴 正悟, 武田 克浩, 藤田 剛, 栗原 英見, 山崎 和久

    日本歯周病学会会誌   60 ( 秋季特別 )   136 - 136   2018年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • 歯根膜細胞における機械刺激による恒常性への影響

    藤田 彩乃, 森松 賢順, 西山 雅祥, 成瀬 恵治, 高柴 正悟

    日本歯周病学会会誌   60 ( 秋季特別 )   117 - 117   2018年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    researchmap

  • 化学療法施行中に口腔乾燥を訴えた進行再発乳がん患者の口腔内所見

    杉浦 裕子, 高橋 麻里子, 畑中 加珠, 田端 雅弘, 高柴 正悟

    日本歯科衛生学会雑誌   13 ( 1 )   126 - 126   2018年8月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本歯科衛生学会  

    researchmap

  • 歯周炎と誤嚥性肺炎の関係

    高柴 正悟

    特定非営利活動法人日本臨床歯周病学会年次大会プログラム・講演抄録集   36回   61 - 62   2018年7月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本臨床歯周病学会  

    researchmap

  • PET(18F-FDG)/CT検査を用いた慢性歯周炎患者における歯周組織炎症の評価

    井手口 英隆, 山城 圭介, 下江 正幸, 山本 直史, 長島 義之, 高柴 正悟

    日本歯周病学会会誌   60 ( 2 )   105 - 116   2018年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯周病学会  

    歯周炎に対する検査方法は多数報告されているが、歯周組織炎症の程度を客観的に評価することが出来る方法は未だに確立されていない。我々は、医科領域で広く用いられているPET(18F-FDG)/CTが炎症組織の局在と炎症強度を可視化することができることに着目した。そして、乳がんの既往を有する慢性歯周炎患者に対する歯周治療の効果を、既存の歯周組織検査方法とPET(18F-FDG)/CTとで経時的に比較検討した。歯周治療が進むに従って歯周組織炎症は消失し、BOPの割合は56%から3%に、PISAは1143mm2から27mm2に改善した。さらに、歯周治療前にPET(18F-FDG)/CTで検出された18F-FDGの顕著な集積は歯周治療後には消失した。これらの結果から、PET(18F-FDG)/CTは炎症性口腔疾患に対する新規検査方法として有用と考えられる。すなわち本症例報告は、PET(18F-FDG)/CTが医科-歯科共通の検査項目となることによって、周術期の医科-歯科連携の強化や、全身疾患を有する多くの歯周炎患者に対してさらに効果的な歯周治療を行うことが出来る可能性を提案するものである。(著者抄録)

    researchmap

    その他リンク: https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2018&ichushi_jid=J01095&link_issn=&doc_id=20180706390006&doc_link_id=%2Fcp9perlo%2F2018%2F006002%2F006%2F0105-0116%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fcp9perlo%2F2018%2F006002%2F006%2F0105-0116%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

  • High Mobility Group Box 1(HMGB1)誘導性の炎症反応はマウス抜歯窩の骨治癒を促進する

    青柳 浩明, 山城 圭介, 平田 千暁, 井手口 英隆, 山崎 睦代, 河村 麻理, 鈴木 里紗, 山本 直史, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   148回   94 - 94   2018年5月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

    researchmap

  • 真菌二次代謝産物(+)-terreinはヒト歯肉上皮細胞におけるAggregatibacter actinomycetemcomitans刺激による細胞間接着分子の発現低下を抑制する

    中村 亜里紗, 大森 一弘, 小林 寛也, 冨川 知子, 山本 総司, 中川 沙紀, 山本 直史, 高柴 正悟

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   148回   153 - 153   2018年5月

     詳細を見る

    記述言語:日本語   出版者・発行元:(NPO)日本歯科保存学会  

    researchmap