Faculty Profiles - SUGIMOTO Seiichiro

MISC - SUGIMOTO Seiichiro

Division display 1 - 20 of about 761 ／ All the affair displays >>

Dynamic Shifts in Inflammation and Fibrotic Remodeling in Post-HSCT Bronchiolitis Obliterans: A Spatial Transcriptomics Study

Akira Yamamoto, Nobuharu Fujii, Keisuke Seike, Seiichiro Sugimoto, Yusuke Naoi, Yui Kambara, Kanako Fujiwara, Toshiki Terao, Tadashi Oyama, Mari Kunihiro, Takumi Kondo, Hiroki Kobayashi, Hideaki Fujiwara, Noboru Asada, Daisuke Ennishi, Keiko Fujii, Shinichi Toyooka, Yoshinobu Maeda

Blood 2024.11

Language：English

<jats:sec>
<jats:title/>
<jats:p>Bronchiolitis obliterans (BO) is a severe complication following hematopoietic stem cell transplantation (HSCT), significantly impacting patients' prognosis and quality of life. Despite its clinical importance, the underlying pathophysiological mechanisms remain poorly understood.</jats:p>
<jats:p>We analyzed lung specimens from three patients who developed BO post-HSCT and subsequently underwent lung transplantation. All patients had acute lymphoblastic leukemia as their primary disease and developed BO 1-2 years after HSCT. Healthy lung regions from two cancer patients served as controls. Using NanoString GeoMx Digital Spatial Profiling, we performed transcriptional profiling with the Human Whole Transcriptome Atlas on 94 regions of interest (ROIs). BO lesions were classified based on our previously established staging system (Kuroi T et al. Int J Hematol. 2021) and analyzed for both inner and outer bronchiolar regions.</jats:p>
<jats:p>Linear mixed model analysis identified significant differential gene expression between BO and control groups. GO enrichment analysis highlighted biological processes such as phagocytosis, positive regulation of leukocyte activation, and cell activation, indicating a close association with immune cells, particularly macrophages, which are known to obstruct bronchioles in BO. Processes such as regulation of cell-cell adhesion and post-Golgi vesicle-mediated transport were also enriched, suggesting involvement in extracellular matrix remodeling and fibroblast activation. GSEA further identified significant processes, including inflammatory response, defense response, positive regulation of response to stimulus, and vesicle-mediated transport. These results suggest an interplay between immune response and tissue remodeling. Notably, changes in the expression of genes such as HES1, IGF2BP2, PTPRF, and ITGB2, which are involved in cell adhesion and cytoskeletal reorganization, were closely associated with fibroblast activation and macrophage infiltration. These findings parallel the fibrosis process observed in BO following lung transplantation, where macrophage infiltration and myofibroblast activation are critical. Given these insights, our focus shifted to the dynamics of macrophages and myofibroblasts, aiming to understand their roles in the progression from inflammation to tissue remodeling.</jats:p>
<jats:p>CD68, a pan-macrophage marker, showed higher expression in Early and Middle-outside stages (Inflammatory Phase) of BO, but significantly decreased in Middle-inside and Late stages (Remodeling Phase) as the disease progressed. Unsupervised clustering analysis revealed distinct gene expression profiles between these phases, suggesting a temporal shift in disease mechanisms as BO progresses from inner bronchioles outwards. Correlation analysis revealed significant associations between CD68 and JAK1/STAT1 expression, as well as genes associated with myofibroblast activity and extracellular matrix remodeling (e.g., ACTA2, TGFB1). These findings suggest a coordinated process of inflammation and remodeling in BO, potentially involving the JAK-STAT pathway.</jats:p>
<jats:p>Linear mixed model analysis revealed distinct gene expression patterns between Inflammatory and Remodeling Phases, characterized by inflammatory/proliferation-related genes (e.g., CXCL6, HLA-family) and tissue remodeling/protein synthesis and genes (e.g., ITGB1, RPL and RPS families), respectively. This transition aligns with changes in macrophage-related gene expression. Pathway analysis revealed enrichment of translation, ribosomal activity, and metabolic processes in the Remodeling Phase, indicating a dynamic shift in biological activities as BO progresses.</jats:p>
<jats:p>In conclusion, this study reveals a transition in BO pathology from inflammation to remodeling, with macrophages playing a central role. The coordinated changes in gene expression provide a comprehensive picture of BO progression. These insights into post-HSCT BO pathophysiology highlight potential therapeutic targets in macrophage function and tissue remodeling pathways, suggesting possibilities for stage-specific interventions in BO treatment.</jats:p>
</jats:sec>

DOI： 10.1182/blood-2024-202251

researchmap

小児肺移植の成果と課題 Invited

杉本誠一郎, 柳光剛志, 富岡泰章, 田中真, 諏澤憲, 枝園和彦, 三好健太郎, 岡崎幹生, 豊岡伸一

日本胸部外科学会定期学術集会(Web) 77th 2024.11

心臓死ドナーからの肺・肝・腎の移植を企図したPulmoabdominal Normothermic Regional Perfusionによる新たな摘出前臓器保護評価法の開発 Invited

田中真, 梅田将志, 柳光剛志, 富岡泰章, 枝園和彦, 諏澤憲, 三好健太郎, 岡崎幹生, 杉本誠一郎, 豊岡伸一

日本胸部外科学会定期学術集会(Web) 77th 2024.11

小児肺移植の問題点 ―小児肺移植センターは必要か？―

大谷真二, 杉本誠一郎, 豊岡伸一, 佐野由文

第86回日本臨床外科学会総会 2024.11

造血幹細胞と同一ドナーからの生体片肺葉移植 Invited

渡邉龍秋, 柳光剛志, 田中里奈, 中島大輔, 杉本誠一郎, 豊岡伸一, 佐藤雅昭, 白石武史, 伊達洋至, 岡田克典

日本胸部外科学会定期学術集会(Web) 77th 2024.11

局所進行肺癌に対する治療を再考する切除可能局所進行肺癌に対する導入化学放射線療法の長期成績

諏澤憲, 岡田和大, 柳光剛志, 富岡泰章, 田中真, 枝園和彦, 三好健太郎, 岡崎幹生, 杉本誠一郎, 豊岡伸一

日本癌治療学会学術集会抄録集 62回 OPD5 - 5 2024.10

肺癌周術期治療の現在と未来術前導入化学放射線療法の経験に基づく当院の肺がん周術期治療の現状と工夫

枝園和彦, 柳光剛志, 富岡泰章, 田中真, 諏澤憲, 三好健太郎, 岡崎幹生, 杉本誠一郎, 豊岡伸一

日本癌治療学会学術集会抄録集 62回 OSY1 - 5 2024.10

MA08.09 Predictive CT Features of Visceral Pleural Invasion in Lung Cancer with Ground Glass Opacity

N. Hayashi, K. Suzawa, K. Okada, T. Ryuko, Y. Tomioka, S. Tanaka, K. Miyoshi, K. Shien, M. Okazaki, S. Sugimoto, S. Toyooka

Journal of Thoracic Oncology 2024.10

縦隔に発生した巨大脱分化型脂肪肉腫の1 切除例

脇翔平, 福本侑麻, 真鍋健太, 森俊介, 柳光剛志, 富岡泰章, 田中真, 突沖貴宏, 諏澤憲, 三好健太郎, 枝園和彦, 岡崎幹生, 杉本誠一郎, 枝園忠彦, 豊岡伸一

第99回中国四国外科学会総会 2024.9

安全なロボット支援下複雑肺区域切除術

岡崎幹生, 富岡泰章, 田中真, 諏澤憲, 枝園和彦, 三好健太郎, 杉本誠一郎, 豊岡伸一

第99回中国四国外科学会総会 2024.9

肺アスペルギルス症に対する手術症例の臨床的特徴と周術期の経過

福本侑麻, 枝園和彦, 脇翔平, 森俊介, 真鍋建太, 柳光剛志, 富岡泰章, 田中真, 諏澤憲, 三好健太郎, 岡﨑幹生, 杉本誠一郎, 豊岡伸一

第99回中国四国外科学会総会 2024.9

臓器移植レシピエントの感染症肺移植レシピエントにおける新型コロナウイルス感染症 5類移行後の現状

妹尾知哉, 杉本誠一郎, 調枝治樹, 柳光剛志, 富岡泰章, 田中真, 石原恵, 堂口佳子, 枝園和彦, 諏澤憲, 三好健太郎, 岡崎幹生, 豊岡伸一

移植 59 ( 総会臨時 ) 161 - 161 2024.9

肺移植後の機能性ディスペプシアに対しPEG-J留置が奏功した一例

柳光剛志, 杉本誠一郎, 田中真, 富岡泰章, 石原恵, 堂口佳子, 枝園和彦, 諏澤憲, 三好健太郎, 岡崎幹生, 豊岡伸一

移植 59 ( 総会臨時 ) 374 - 374 2024.9

肺移植後の抗体関連型拒絶反応に対する免疫グロブリン製剤使用の実態調査

中島大輔, 伊達洋至, 松原慧, 杉本誠一郎, 白石武史, 平間崇, 芳川豊史, 中川健, 江川裕人

移植 59 ( 総会臨時 ) 265 - 265 2024.9

再肺移植後急性期に膵頭部癌による出血性膵炎を繰り返したNightmare case

林龍也, 田中真, 石原恵, 堂口佳子, 梅田将志, 調枝治樹, 柳光剛志, 富岡泰章, 枝園和彦, 諏澤憲, 三好健太郎, 岡崎幹生, 杉本誠一郎, 豊岡伸一

移植 59 ( 総会臨時 ) 255 - 255 2024.9

両側脳死肺移植後のサイトメガロウイルス感染症の予防にレテルモビルが有効だった一例

俣野貴慶, 杉本誠一郎, 田中真, 柳光剛志, 調枝治樹, 富岡泰章, 堂口佳子, 石原恵, 諏澤憲, 枝園和彦, 三好健太郎, 岡崎幹生, 豊岡伸一

移植 59 ( 総会臨時 ) 373 - 373 2024.9

脳死片肺移植後の抗体関連型拒絶反応に対する集学的治療の経験

調枝治樹, 杉本誠一郎, 柳光剛志, 富岡泰章, 田中真, 堂口佳子, 石原恵, 枝園和彦, 諏澤憲, 三好健太郎, 岡崎幹生, 豊岡伸一

移植 59 ( 総会臨時 ) 294 - 294 2024.9

肺移植後長期管理 Up to date 肺移植後の慢性腎臓病に対する長期管理 up to date

杉本誠一郎, 柳光剛志, 富岡泰章, 田中真, 石原恵, 堂口佳子, 諏澤憲, 枝園和彦, 三好健太郎, 岡崎幹生, 豊岡伸一

移植 59 ( 総会臨時 ) 173 - 173 2024.9

症例から学ぶディスカッション(呼吸器外科) 片側生体肺移植後の肺高血圧症に対する治療戦略 Invited

杉本誠一郎

日本肺高血圧・肺循環学会学術集会抄録集 9回 96 - 96 2024.8

右上葉肺動脈のみが残存する右孤立性先天性肺動脈欠損を合併した右下葉肺癌の1手術例

岡田和大, 田中真, 柳光剛志, 富岡泰章, 諏澤憲, 枝園和彦, 三好健太郎, 岡崎幹生, 杉本誠一郎, 豊岡伸一

第70回日本呼吸器学会中国・四国地方会第62回日本肺癌学会中国・四国支部学術集会 2024.7

　 PREV - NEXT 　

< SUGIMOTO Seiichiro >

MISC - SUGIMOTO Seiichiro