Updated on 2021/07/12

写真a

 
UEDA Hitoshi
 
Organization
Natural Science and Technology Professor
Position
Professor
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Degree

  • 農学修士 ( 東北大学 )

  • 農学博士 ( 東北大学 )

Research Interests

  • Molecular Biology

  • Developmental Biology

  • 分子生物学

  • 発生生物学

Research Areas

  • Life Science / Developmental biology

  • Life Science / Molecular biology

Education

  • Tohoku University   農学研究科  

    - 1985

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    Country: Japan

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  • Tohoku University    

    - 1985

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  • Tohoku University   Faculty of Agriculture  

    - 1980

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    Country: Japan

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  • Tohoku University    

    - 1980

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Research History

  • - Professor,Graduate School of Natural Science and Technology,Okayama University

    2004

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  • - 岡山大学自然科学研究科 教授

    2004

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Professional Memberships

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Papers

  • Proteasome activity determines pupation timing through the degradation speed of timer molecule Blimp-1. Reviewed

    Aly H, Akagi K, Ueda H

    Development, growth & differentiation   60 ( 8 )   502 - 508   2018.10

  • Protease resistance of porcine acidic mammalian chitinase under gastrointestinal conditions implies that chitin-containing organisms can be sustainable dietary resources Reviewed

    Eri Tabata, Akinori Kashimura, Satoshi Wakita, Misa Ohno, Masayoshi Sakaguchi, Yasusato Sugahara, Yasutada Imamura, Shiro Seki, Hitoshi Ueda, Vaclav Matoska, Peter O. Bauer, Fumitaka Oyama

    SCIENTIFIC REPORTS   7 ( 1 )   12963   2017.10

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:NATURE PUBLISHING GROUP  

    Chitin, a polymer of N-acetyl-D-glucosamine (GlcNAc), is a major structural component in chitin-containing organism including crustaceans, insects and fungi. Mammals express two chitinases, chitotriosidase (Chit1) and acidic mammalian chitinase (AMCase). Here, we report that pig AMCase is stable in the presence of other digestive proteases and functions as chitinolytic enzyme under the gastrointestinal conditions. Quantification of chitinases expression in pig tissues using quantitative real-time PCR showed that Chit1 mRNA was highly expressed in eyes, whereas the AMCase mRNA was predominantly expressed in stomach at even higher levels than the housekeeping genes. AMCase purified from pig stomach has highest activity at pH of around 2-4 and remains active at up to pH 7.0. It was resistant to robust proteolytic activities of pepsin at pH 2.0 and trypsin and chymotrypsin at pH 7.6. AMCase degraded polymeric chitin substrates including mealworm shells to GlcNAc dimers. Furthermore, we visualized chitin digestion of fly wings by endogenous AMCase and pepsin in stomach extract. Thus, pig AMCase can function as a protease resistant chitin digestive enzyme at broad pH range present in stomach as well as in the intestine. These results indicate that chitin-containing organisms may be a sustainable feed ingredient in pig diet.

    DOI: 10.1038/s41598-017-13526-6

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  • A biological timer in the fat body comprising Blimp-1, beta Ftz-f1 and Shade regulates pupation timing in Drosophila melanogaster Reviewed

    Kazutaka Akagi, Moustafa Sarhan, Abdel-Rahman S. Sultan, Haruka Nishida, Azusa Koie, Takumi Nakayama, Hitoshi Ueda

    DEVELOPMENT   143 ( 13 )   2410 - 2416   2016.7

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:COMPANY OF BIOLOGISTS LTD  

    During the development of multicellular organisms, many events occur with precise timing. In Drosophila melanogaster, pupation occurs about 12 h after puparium formation and its timing is believed to be determined by the release of a steroid hormone, ecdysone (E), from the prothoracic gland. Here, we demonstrate that the ecdysone-20-monooxygenase Shade determines pupation timing by converting E to 20-hydroxyecdysone (20E) in the fat body, which is the organ that senses nutritional status. The timing of shade expression is determined by its transcriptional activator beta Ftz-f1. The beta ftz-f1 gene is activated after a decline in the expression of its transcriptional repressor Blimp-1, which is temporally expressed around puparium formation in response to a high titer of 20E. The expression level and stability of Blimp-1 is critical for the precise timing of pupation. Thus, we propose that Blimp-1 molecules function like sand in an hourglass in this precise developmental timer system. Furthermore, our data suggest that a biological advantage results from both the use of a transcriptional repressor for time determination and the association of developmental timing with nutritional status of the organism.

    DOI: 10.1242/dev.133595

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  • SMARCAD1 is an ATP-dependent stimulator of nucleosomal H2A acetylation via CBP, resulting in transcriptional regulation Reviewed

    Masamichi Doiguchi, Takeya Nakagawa, Yuko Imamura, Mitsuhiro Yoneda, Miki Higashi, Kazuishi Kubota, Satoshi Yamashita, Hiroshi Asahara, Midori Iida, Satoshi Fujii, Tsuyoshi Ikura, Ziying Liu, Tulip Nandu, W. Lee Kraus, Hitoshi Ueda, Takashi Ito

    SCIENTIFIC REPORTS   6   20179   2016.2

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:NATURE PUBLISHING GROUP  

    Histone acetylation plays a pivotal role in transcriptional regulation, and ATP-dependent nucleosome remodeling activity is required for optimal transcription from chromatin. While these two activities have been well characterized, how they are coordinated remains to be determined. We discovered ATP-dependent histone H2A acetylation activity in Drosophila nuclear extracts. This activity was column purified and demonstrated to be composed of the enzymatic activities of CREB-binding protein (CBP) and SMARCAD1, which belongs to the Etl1 subfamily of the Snf2 family of helicase-related proteins. SMARCAD1 enhanced acetylation by CBP of H2A K5 and K8 in nucleosomes in an ATP-dependent fashion. Expression array analysis of S2 cells having ectopically expressed SMARCAD1 revealed up-regulated genes. Using native genome templates of these up-regulated genes, we found that SMARCAD1 activates their transcription in vitro. Knockdown analysis of SMARCAD1 and CBP indicated overlapping gene control, and ChIP-seq analysis of these commonly controlled genes showed that CBP is recruited to the promoter prior to SMARCAD1. Moreover, Drosophila genetic experiments demonstrated interaction between SMARCAD1/Etl1 and CBP/nej during development. The interplay between the remodeling activity of SMARCAD1 and histone acetylation by CBP sheds light on the function of chromatin and the genome-integrity network.

    DOI: 10.1038/srep20179

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  • Autocrine regulation of ecdysone synthesis by beta 3-octopamine receptor in the prothoracic gland is essential for Drosophila metamorphosis Reviewed

    Yuya Ohhara, Yuko Shimada-Niwa, Ryusuke Niwa, Yasunari Kayashima, Yoshiki Hayashi, Kazutaka Akagi, Hitoshi Ueda, Kimiko Yamakawa-Kobayashi, Satoru Kobayashi

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   112 ( 5 )   1452 - 1457   2015.2

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:NATL ACAD SCIENCES  

    In Drosophila, pulsed production of the steroid hormone ecdysone plays a pivotal role in developmental transitions such as metamorphosis. Ecdysone production is regulated in the prothoracic gland (PG) by prothoracicotropic hormone (PTTH) and insulin-like peptides (Ilps). Here, we show that monoaminergic autocrine regulation of ecdysone biosynthesis in the PG is essential for metamorphosis. PG-specific knockdown of a monoamine G protein-coupled receptor, beta 3-octopamine receptor (Oct beta 3R), resulted in arrested metamorphosis due to lack of ecdysone. Knockdown of tyramine biosynthesis genes expressed in the PG caused similar defects in ecdysone production and metamorphosis. Moreover, PTTH and Ilps signaling were impaired by Oct beta 3R knockdown in the PG, and activation of these signaling pathways rescued the defect in metamorphosis. Thus, monoaminergic autocrine signaling in the PG regulates ecdysone biogenesis in a coordinated fashion on activation by PTTH and Ilps. We propose that monoaminergic autocrine signaling acts downstream of a body size checkpoint that allows metamorphosis to occur when nutrients are sufficiently abundant.

    DOI: 10.1073/pnas.1414966112

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  • Function of the nuclear receptor FTZ-F1 during the pupal stage in Drosophila melanogaster Reviewed

    Abdel-Rahman S. Sultan, Yasuhiro Oish, Hitoshi Ueda

    DEVELOPMENT GROWTH & DIFFERENTIATION   56 ( 3 )   245 - 253   2014.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-BLACKWELL  

    The nuclear receptor FTZ-F1 is expressed in most cells in a temporally specific manner, and its expression is induced immediately after decline in ecdysteroid levels. This factor plays important roles during embryogenesis, larval ecdysis, and early metamorphic stages. However, little is known about the expression pattern, regulation and function of this receptor during the pupal stage. We analyzed the expression pattern and regulation of ftz-f1 during the pupal period, as well as the phenotypes of RNAi knockdown or mutant animals, to elucidate its function during this stage. Western blotting revealed that FTZ-F1 is expressed at a high level during the late pupal stage, and this expression is dependent on decreasing ecdysteroid levels. By immunohistological analysis of the late pupal stage, FTZ-F1 was detected in the nuclei of most cells, but cytoplasmic localization was observed only in the oogonia and follicle cells of the ovary. Both the ftz-f1 genetic mutant and temporally specific ftz-f1 knockdown using RNAi during the pupal stage showed defects in eclosion and in the eye, the antennal segment, the wing and the leg, including bristle color and sclerosis. These results suggest that FTZ-F1 is expressed in most cells at the late pupal stage, under the control of ecdysteroids and plays important roles during pupal development.

    DOI: 10.1111/dgd.12125

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  • The Binding of Multiple Nuclear Receptors to a Single Regulatory Region Is Important for the Proper Expression of EDG84A in Drosophila melanogaster Reviewed

    Kazutaka Akagi, Yuji Kageyama, Yasunari Kayashima, Yusuke Takakura, Susumu Hirose, Hitoshi Ueda

    JOURNAL OF MOLECULAR BIOLOGY   425 ( 1 )   71 - 81   2013.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD  

    Nuclear receptor transcription factor family members share target sequence similarity; however, little is known about how these factors exert their specific regulatory control. Here, we examine the mechanism regulating the expression of the Drosophila EDG84A gene, a target gene of the orphan nuclear receptor beta FTZ-F1, as a model to study the cooperative behavior among nuclear receptors. We show that the three nuclear receptors beta FTZ-F1, DHR3, and DHR39 bind to a common element in the EDG84A promoter. The expression level of the EDG84A promoter-lacZ reporter genes in DHR39-induced and mutant animals, respectively, suggests that DHR39 works as a repressor. The activity of a reporter gene carrying a mutation preventing DHR3 binding was reduced in ftz-f1 mutants and rescued by the induced expression of beta FTZ-F1, suggesting that DHR3 and beta FTZ-F1 activate the EDG84A gene in a redundant manner. A reporter gene carrying a mutation that abolishes DHR39 and FTZ-F1 binding was prematurely expressed, and the expression level of the reporter gene carrying a mutation preventing DHR3 binding was reduced. These findings suggest that the temporal expression of this gene is mainly controlled by beta FTZ-F1 but that the binding of DHR3 is also important. Comparison of the binding site sequence among Drosophila species suggests that DHR3 binding ability was gained after the melanogastersubgroup evolved, and this ability may contribute to the robust expression of this gene. These results show the complicated regulatory mechanisms utilized by multiple nuclear receptors to properly regulate the expression of their target gene through a single target site. (C) 2012 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.jmb.2012.10.020

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  • Regulatory mechanisms of ecdysone-inducible Blimp-1 encoding a transcriptional repressor that is important for the prepupal development in Drosophila Reviewed

    Kazutaka Akagi, Hitoshi Ueda

    DEVELOPMENT GROWTH & DIFFERENTIATION   53 ( 5 )   697 - 703   2011.6

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    Blimp-1 is an ecdysone-inducible transcription factor that is expressed in the early stage of the prepupal period. The timing of its disappearance determines expression timing of the FTZ-F1 gene, whose temporally restricted expression is essential for the prepupal development. To elucidate the termination mechanism of Blimp-1 gene expression, we examined the regulation of the Blimp-1 gene using an organ culture system. The results showed that the Blimp-1 gene is transcribed in cultured organs taken from a low ecdysteroid period even after extended exposure to 20-hydroxyecdysone, while well-known early genes such as E75A are repressed under the same conditions. Similar selective transcription was observed in the cultured organs obtained from a high ecdysteroid period. We further showed that Blimp-1 transcripts quickly disappeared in the presence of actinomycin D. From these results, we concluded that the Blimp-1 gene is transcribed when the ecdysteroid titer is high, but the expressed mRNA degrades rapidly; these unique regulations limit its expression to the high ecdysteroid stage.

    DOI: 10.1111/j.1440-169X.2011.01276.x

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  • Midline governs axon pathfinding by coordinating expression of two major guidance systems Reviewed

    Qing-Xin Liu, Masaki Hiramoto, Hitoshi Ueda, Takashi Gojobori, Yasushi Hiromi, Susumu Hirose

    GENES & DEVELOPMENT   23 ( 10 )   1165 - 1170   2009.5

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT  

    Formation of the neural network requires concerted action of multiple axon guidance systems. How neurons orchestrate expression of multiple guidance genes is poorly understood. Here, we show that Drosophila T-box protein Midline controls expression of genes encoding components of two major guidance systems: Frazzled, ROBO, and Slit. In midline mutant, expression of all these molecules are reduced, resulting in severe axon guidance defects, whereas misexpression of Midline induces their expression. Midline is present on the promoter regions of these genes, indicating that Midline controls transcription directly. We propose that Midline controls axon pathfinding through coordinating the two guidance systems.

    DOI: 10.1101/gad.1774209

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  • Intraspecific variation in the Egyptian scorpion Scorpio maurus palmatus venom collected from different biotopes Reviewed

    Mohamed A. Abdel-Rahman, Mohamed Alaa A. Omran, Ismail M. Abdel-Nabi, Hitoshi Ueda, Alistair McVean

    TOXICON   53 ( 3 )   349 - 359   2009.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PERGAMON-ELSEVIER SCIENCE LTD  

    The present study was conducted to explore the following hypotheses: (i) do scorpions (Scorpio maurus palmatus) from different biotopes exhibit intraspecific diversity in their venom? (ii) if so, is this variation associated with ecological or genetic factors, geographical distance, and/or multiple interrelated parameters? To address these questions, scorpions were collected from four geographically isolated localities in Egypt. Three of these locations are from mutually isolated pockets in the arid biotope of Southern Sinai (Wadi Sahab, El-Agramia and Rahaba plains). The fourth population was sampled from the semiarid biotope of Western Mediterranean Costal Desert (WMCD). Using reducing gel electrophoresis (SDS-PAGE), we have shown biotope-specific variation in the expression of peptides from scorpions collected from these distinct areas. WMCD sourced venom samples contain higher molecular weight protein components (219, 200,170,139,116 kDa) than Southern Sinai scorpion venom samples. The Southern Sinai venom is characterized by the presence of 11 protein bands (93-0.58 kDa) that are not mirrored in the individual venom samples of WMCD. Bands of 33 and 3.4 kDa were characteristics of all individual venom samples of the scorpion populations, Even within Southern Sinai area, Sahab venom contains five fractions that are not detected in both El-Agramia and Rahaba venom samples. Moreover, male and female venom analysis revealed some sex-related proteomic similarities and differences between scorpion populations. Female venom appears to be more complicated than the male venom. Female venom samples showed bands of 219, 200, 77.5, 55.5, 45, 39, 37, 24 and 16 kDa which were absent in the male venom. The random amplified polymorphic DNA (RAPD) technique was used to estimate the genetic distance between the four scorpion populations. The RAPD data confirmed the genetic diversity at molecular level among the sampled populations. More than 77 RAPD bands (ranging in size from 125 to 15,000 bp) were defined from the four scorpion populations. Of the 77 bands, 57 (76.2%) were polymorphic and 20 were monomorphic among the populations. The similarity coefficient data of venom and DNA were used to construct separate dendrograms, which grouped together the Southern Sinai populations and these were some distance away from the WMCD population. Taken together, we suspect that a combination of local environmental conditions, geographical separation and genetic separation may play a major role in the intraspecific variation of venom of S. m. palmatus. (C) 2008 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.toxicon.2008.12.007

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  • Drosophila blimp-1 is a transient transcriptional repressor that controls timing of the ecdysone-induced developmental pathway Reviewed

    Yasuo Agawa, Moustafa Sarhan, Yuji Kageyama, Kazutaka Akagi, Masayoshi Takai, Kazuya Hashiyama, Tadashi Wada, Hiroshi Handa, Akihiro Iwamatsu, Susumu Hirose, Hitoshi Ueda

    MOLECULAR AND CELLULAR BIOLOGY   27 ( 24 )   8739 - 8747   2007.12

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:AMER SOC MICROBIOLOGY  

    Regulatory mechanisms controlling the timing of developmental events are crucial for proper development to occur. ftz-fl is expressed in a temporally regulated manner following pulses of ecdysteroid and this precise expression is necessary for the development of Drosophila melanogaster. To understand how insect hormone ecdysteroids regulate the timing of FTZ-F1 expression, we purified a DNA binding regulator of ftz-fl. Mass spectroscopy analysis revealed this protein to be a fly homolog of mammalian B lymphocyte-induced maturation protein 1 (Blimp-1). Drosophila Blimp-1 (dBlimp-1) is induced directly by 20-hydroxyecdysone, and its product exists during high-ecdysteroid periods and turns over rapidly. Forced expression of dBlimp-1 and RNA interference analysis indicate that dBlimp-1 acts as a repressor and controls the timing of FTZ-F1 expression. Furthermore, its prolonged expression results in delay of pupation timing. These results suggest that the transient transcriptional repressor dBlimp-1 is important for determining developmental timing in the ecdysone-induced pathway.

    DOI: 10.1128/MCB.01304-07

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  • A simple and quick method to isolate nuclear extracts from pupae of Drosophila melanogaster. Reviewed

    H Kawasaki, S Hirose, H Ueda

    CYTOTECHNOLOGY   49 ( 1 )   67 - 70   2005.9

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    Preparation of nuclear extracts is a critical step for biochemical identification of factors which function in the nuclei such as transcription factors. We have established a new method to prepare nuclear extracts from prepupae or pupae of Drosophila melanogaster. The method is simple and particularly useful for small-scale isolation from materials that are hard to get in large amounts such as specific tissues or animal bodies covered by pupal cases.

    DOI: 10.1007/s10616-005-5414-3

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  • [Gene cascade by hormonal control during development of insects]. Reviewed

    Ueda H

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   48 ( 16 )   2254 - 2260   2003.12

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  • Drosophila FACT contributes to Hox gene expression through physical and functional interactions with GAGA factor Reviewed

    T Shimojima, M Okada, T Nakayama, H Ueda, K Okawa, A Iwamatsu, H Handa, S Hirose

    GENES & DEVELOPMENT   17 ( 13 )   1605 - 1616   2003.7

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT  

    Chromatin structure plays a critical role in the regulation of transcription. Drosophila GAGA factor directs chromatin remodeling to its binding sites. We show here that Drosophila FACT (facilitates chromatin transcription), a heterodimer of dSPT16 and dSSRP1, is associated with GAGA factor through its dSSRP1 subunit, binds to a nucleosome, and facilitates GAGA factor-directed chromatin remodeling. Moreover, genetic interactions between Trithorax-like encoding GAGA factor and spt16 implicate the GAGA factor-FACT complex in expression of Hox genes Ultrabithorax, Sex combs reduced, and Abdominal-B. Chromatin immunoprecipitation experiments indicated the presence of the GAGA factor-FACT complex in the regulatory regions of Ultrabithorax and Abdominal-B. These data illustrate a crucial role of FACT in the modulation of chromatin structure for the regulation of gene expression.

    DOI: 10.1101/gad.1086803

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  • Cloning of Cyc (Bmal1) homolog in Bombyx mori: structural analysis and tissue specific distributions. Reviewed

    Markova EP, Ueda H, Sakamoto K, Oishi K, Shimada T, Takeda M

    Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology   134 ( 3 )   535 - 542   2003.3

  • Drosophila MBF1 is a co-activator for Tracheae Defective and contributes to the formation of tracheal and nervous systems Reviewed

    QX Liu, M Jindra, H Ueda, Y Hiromi, S Hirose

    DEVELOPMENT   130 ( 4 )   719 - 728   2003.2

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:COMPANY OF BIOLOGISTS LTD  

    During gene activation, the effect of binding of transcription factors to cis-acting DNA sequences is transmitted to RNA polymerase by means of co-activators. Although co-activators contribute to the efficiency of transcription, their developmental roles are poorly understood. We used Drosophila to conduct molecular and genetic dissection of an evolutionarily conserved but unique co-activator, Multiprotein Bridging Factor 1 (MBF1), in a multicellular organism. Through immunoprecipitation, MBF1 was found to form a ternary complex including MBF1, TATA-binding protein (TBP) and the bZIP protein Tracheae Defective (TDF)/Apontic. We have isolated a Drosophila mutant that lacks the mbf1 gene in which no stable association between TBP and TDF is detectable, and transcription of a TDF-dependent reporter gene is reduced by 80%. Although the null mutants of mbf1 are viable, tdf becomes haploinsufficient in mbf1-deficient background, causing severe lesions in tracheae and the central nervous system, similar to those resulting from a complete loss of tdf function. These data demonstrate a crucial role of MBF1 in the development of tracheae and central nervous system.

    DOI: 10.1242/dev.00297

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  • beta FTZ-F1 dependent and independent activation of Edg78E, a pupal cuticle gene, during the early metamorphic period in Drosophila melanogaster Reviewed

    H Kawasaki, S Hirose, H Ueda

    DEVELOPMENT GROWTH & DIFFERENTIATION   44 ( 5 )   419 - 425   2002.10

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:BLACKWELL PUBLISHING ASIA  

    Insect metamorphosis is a developmentally important event for formation of adult structures from larval imaginal cells, and it is controlled by the ecdysteroid hormone. At the onset of metamorphosis, both the cuticle gene Edg78E and the transcription factor betaFTZ-F1 are expressed during the mid- to late prepupal period after a large ecdysteroid pulse. Edg78E mRNA is inducible by premature expression of betaFTZ-F1 and the Edg78E expression level is reduced in an ftz-f1 mutant. Using a transgenic fly reporter assay, a 1.2 kb promoter region of the Edg78E gene has been identified, which was sufficient for appropriate temporally and spatially specific expression of the reporter gene LacZ. Within the promoter region, two betaFTZ-F1 binding sites are present and disruption of these sites reduced the expression level of the reporter gene. LacZ expression levels were dramatically reduced in the head and thorax regions but not affected in the abdominal region, suggesting that betaFTZ-F1 is required for high-level Edg78E expression specifically in the head and thorax regions. The findings suggest that betaFTZ-F1 is a regulator for temporal gene expression at the onset of metamorphosis, and that complex mechanisms regulate the temporal and spatial regulation of gene expression during metamorphosis.

    DOI: 10.1046/j.1440-169x.2002.00655.x

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  • MBF2 is a tissue- and stage-specific coactivator that is regulated at the step of nuclear transport in the silkworm Bombyx mori

    Qing-Xin Liu, Hitoshi Ueda, Susumu Hirose

    Developmental Biology   225 ( 2 )   437 - 446   2000.9

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Academic Press Inc.  

    Coactivators MBF1 and MBF2 mediate BmFTZ-F1-dependent transcriptional activation in vitro by interconnecting BmFTZ-F1, TATA binding protein TBP, and TFIIA. Here, we analyzed temporal and spatial expression patterns of MBF2 during embryonic and larval development of the silkworm Bombyx mori. MBF2 was detected in unfertilized eggs and embryos until stage 26. In stage 22 embryos, MBF1, MBF2, and BmFTZ-F1 colocalize in neural cells. During the larval stage, MBF2 was not expressed in the fat body and trachea. In the silk gland, MBF2 mRNA was constitutively expressed, but MBF2 protein appeared in the period between the second day and the molting D3 stage in both the third and the fourth instars and then disappeared. MBF2 was also detected on the second and third days of the fifth instar. Immunostaining during the fourth molt showed that MBF1, MBF2, and BmFTZ-F1 localize in the nucleus only at the D3 stage, while the two cofactors are present in the cytoplasm at other stages. Immunoprecipitation experiments suggested that MBF1, MBF2, and BmFTZ-F1 form a complex at the D3 stage. Transient expression of these factors in Schneider cell line 2 revealed that MBF1 and MBF2 localize to the nucleus and enhance BmFTZ-F1-dependent transcription only when all three factors are present. These data illustrate the functional regulation of MBF1 and MBF2 at the step of nuclear transport and implicate MBF2 in tissue- and stage-specific transcription. (C) 2000 Academic Press.

    DOI: 10.1006/dbio.2000.9836

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  • Identification of the core domain and the secondary structure of the transcriptional coactivator MBF-1

    Jun Ozaki, Ken-Ichi Takemaru, Takahisa Ikegami, Masaki Mishima, Hitoshi Ueda, Susumu Hirose, Yasuaki Kabe, Hiroshi Handa, Masahiro Shirakawa

    Genes to Cells   4 ( 7 )   415 - 424   1999

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    Background: Multiprotein bridging factor 1 (MBF1) is a transcriptional coactivator necessary for transcriptional activation caused by DNA binding activators, such as FTZ-F1 and GCN4. MBF1 bridges the DNA-binding regions of these activators and the TATA-box binding protein (TBP), suggesting that MBF1 functions by recruiting TBP to promoters where the activators are bound. In addition, MBF1 stimulates DNA binding activities of the activators to their recognition sites. To date, little is known about structures of coactivators that bind to TBP. Results: The two-dimensional (2D) 1H-15N correlation spectrum of 15N labeled MBF1 indicated that MBF1 consists of both flexible and well structured parts. Limited digestion of MBF1 by α-chymotrypsin yielded a ~9 kDa fragment. N-terminal sequence analysis and NMR measurements revealed that this fragment originates from the C-terminal 80 residues of MBF1 and form a well structured C-terminal domain of MBF1, MBF1(CTD). As previous deletion analyses have shown that MBF1(CTD) is capable of binding to TBP, it is suggested that MBF1(CTD) is the TBP binding domain of MBF1. Sequential assignments have been obtained by means of three-dimensional (3D) and four dimensional (4D) heteronuclear correlation spectroscopies, and then the secondary structure of MBF1(CTD) was determined. As a result, MBF1(CTD) was shown to contain four amphipathic helices and a conserved C-terminal region. Asp106 which is assumed to be responsible for the binding to TBP is located at the hydrophilic side of the third helix. Conclusions: Structural analyses revealed that MBF1 consists of two structurally different domains. A N-terminal region is indispensable for the binding to activators, and does not form a well defined structure. In contrast, the C-terminal 80 residues, which is capable of binding to TBP by itself, form a well-structured domain, MBF1(CTD). MBF1(CTD) is made up of four amphipathic helices and a conserved C-terminal tail. A putative TBP binding residue is located on the hydrophilic surface of the third helix.

    DOI: 10.1046/j.1365-2443.1999.00267.x

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  • Resonance assignments, secondary structure and 15N relaxation data of the human transcriptional coactivator hMBF1 (57-148)

    Masaki Mishima, Jun Ozaki, Takahisa Ikegami, Yasuaki Kabe, Masahide Goto, Hitoshi Ueda, Susumu Hirose, Hiroshi Handa, Masahiro Shirakawa

    Journal of Biomolecular NMR   14 ( 4 )   373 - 376   1999

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    Language:English   Publishing type:Research paper (scientific journal)  

    Multiprotein bridging factor 1 (MBF1) is a transcriptional coactivator that is thought to bridge between the TATA box-binding protein (TBP) and DNA binding regulatory factors, and is conserved from yeast to human. Human MBF1 (hMBF1) can bind to TBP and to the nuclear receptor Ad4BP, and is suggested to mediate Ad4BP-dependent transcriptional activation. Here we report the resonance assignments and secondary structure of hMBF1 (57-148) that contains both TBP binding and activator binding residues. 15N relaxation data were also obtained. As a result, hMBF1 (57-148) was shown to consist of flexible N-terminal residues and a C-terminal domain. The C-terminal domain contains four helices and a conserved C-terminal region.

    DOI: 10.1023/A:1008347729176

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  • Comparison of sequences of a transcriptional coactivator MBF2 from three lepidopteran species Bombyx mori, Bombyx mandarina and Samia cynthia

    Qing-Xin Liu, Hitoshi Ueda, Susumu Hirose

    Gene   220 ( 1-2 )   55 - 59   1998.10

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    MBF2 was first isolated from the silkworm Bombyx mori as a positive cofactor that activates transcription through its interaction with TFIIA. To identify conserved domain(s) within the MBF2 molecule, we isolated cDNAs encoding MBF2 homologues from other silkworms Bombyx mandarina and Samia cynthia. Bacterially expressed and purified MBF2 of B. mandarina and S. cynthia activated transcription in vitro. The predicted amino acid sequences of MBF2 from two Bombyx species share 97% homology. When we compared between B. mori and S. cynthia factors, the homology reduced to 50%. Four regions in MBF2 are conserved among these three species. Two of them are present in the middle region of MBF2 that is essential for the transcriptional activation.

    DOI: 10.1016/S0378-1119(98)00428-4

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  • Multiprotein bridging factor 1 (MBF1) is an evolutionary conserved transcriptional coactivator that connects a regulatory factor and TATA element-binding protein

    Ken-Ichi Takemaru, Feng-Qian Li, Hitoshi Ueda, Susumu Hirose

    Proceedings of the National Academy of Sciences of the United States of America   94 ( 14 )   7251 - 7256   1997.7

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:National Academy of Sciences  

    Multiprotein bridging factor 1 (MBF1) is a transcriptional cofactor that bridges between the TATA box-binding protein (TBP) and the Drosophila melanogaster nuclear hormone receptor FTZ-F1 or its silkworm counterpart BmFTZ-F1. A cDNA clone encoding MBF1 was isolated from the silkworm Bombyx mori whose sequence predicts a basic protein consisting of 146 amino acids. Bacterially expressed recombinant MBF1 is functional in interactions with TBP and a positive cofactor MBF2. The recombinant MBF1 also makes a direct contact with FTZ-F1 through the C-terminal region of the FTZ-F1 DNA-binding domain and stimulates the FTZ-F1 binding to its recognition site. The central region of MBF1 (residues 35-113) is essential for the binding of FTZ-F1, MBF2, and TBP. When the recombinant MBF1 was added to a HeLa cell nuclear extract in the presence of MBF2 and FTZ622 bearing the FTZ-F1 DNA-binding domain, it supported selective transcriptional activation of the fushi tarazu gene as natural MBF1 did. Mutations disrupting the binding of FTZ622 to DNA or MBF1, or a MBF2 mutation disrupting the binding to MBF1, all abolished the selective activation of transcription. These results suggest that tethering of the positive cofactor MBF2 to a FTZ-F1-binding site through FTZ-F1 and MBF1 is essential for the binding site-dependent activation of transcription. A homology search in the databases revealed that the deduced amino acid sequence of MBF1 is conserved across species from yeast to human.

    DOI: 10.1073/pnas.94.14.7251

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  • Transcriptional activation through interaction of MBF2 with TFIIA

    Feng-Qian Li, Ken-Ichi Takemaru, Masahide Goto, Hitoshi Ueda, Hiroshi Handa, Susumu Hirose

    Genes to Cells   2 ( 2 )   143 - 153   1997

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Blackwell Publishing Ltd.  

    Background: Transcriptional activation of the Drosopohila melanogaster fushi tarzu gene by FTZ-F1 or its silkworm counterpart BmFTZ-F1 requires two cofactors MBF1 and MBF2 which do not directly bind to DNA. MBF1 is a bridging molecule that connects FTZ-F1 (or BmFTZ- F1), MBF2 and TATA binding protein TBP. MBF2 is a positive cofactor that activates transcription. Results: To elucidate the mechanism of transcriptional activation by MBF2, we isolated a cDNA coding for the factor. Northern blot analyses showed temporally restricted expression of MBF2 mRNA similar to that of BmFTZ-F1 mRNA. The cDNA sequence predicts a polypeptide of 10 kDa whereas natural MBF2 is a glycoprotein of 22 kDa. The deduced amino acid sequence of the factor showed no homology with proteins in the databases. Farwestern analyses and glutathione S-transferase interaction assays demonstrated that MBF2 makes a direct contact with the β-subunit of TFIIA. In a HeLa cell nuclear extract, bacterially expressed recombinant MBF2 activated transcription from various promoters as natural MBF2 did. This activation requires the MBF2-TFIIA interaction. When recombinant MBF2 was added to the HeLa cell nuclear extract in the presence of MBF1 and FTZ622 bearing the DNA-binding region of FTZ-F1, it selectively activated transcription of the fushi tarazu gene. This selective activation also requires the MBF2-TFIIA interaction. Conclusion: MBF2 activates transcription through its interaction with TFIIA. Selective transcriptional activation occurs when MBF2 is recruited to a promoter carrying the FTZ-F1 binding site by FTZ-F1 and MBF1. © Blackwell Science Limited.

    DOI: 10.1046/j.1365-2443.1997.1090306.x

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  • FTZ-F1, a steroid hormone receptor-like protein implicated in the activation of fushi tarazu

    Giovanni Lavorgna, Hitoshi Ueda, Joachim Clos, Carl Wu

    Science   252 ( 5007 )   848 - 851   1991

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    The Drosophila homeobox segmentation gene fushi tarazu (ftz) is expressed in a seven-stripe pattern during early embryogenesis. This characteristic pattern is largely specified by the zebra element located immediately upstream of the ftz transcriptional start site. The FTZ-F1 protein, one of multiple DNA binding factors that interacts with the zebra element, is implicated in the activation of ftz transcription, especially in stripes 1,2,3, and 6. An FTZ-F1 complementary DNA has been cloned by recognition site screening of a Drosophila expression library. The identity of the FTZ-F1 complementary DNA clone was confirmed by immunological cross-reaction with antibodies to FTZ-F1 and by sequence analysis of peptides from purified FTZ-F1 protein. The predicted amino acid sequence of FTZ-F1 revealed that the protein is a member of the nuclear hormone receptor superfamily. This finding raises the possibility that a hormonal ligand affects the expression of a homeobox segmentation gene early in embryonic development.

    DOI: 10.1126/science.1709303

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  • Identification and purification of a Bombyx mori homologue of FTZ-F1

    Hitoshi Ueda, Susumu Hirose

    Nucleic Acids Research   18 ( 24 )   7229 - 7234   1990

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    Extracts from embryos and from posterior and middle silk glands of the silkworm, Bombyx mori contain a sequence specific DNA binding factor termed BmFTZ-F1. The factor binds to the recognition site of FTZ-F1, a positive regulator of the fushi tarazu gene in Drosophlla melanogaster. BmFTZ-F1 and FTZ-F1 share the same methylation interference patterns, the same chromatographic behaviors and similar protease digestion profiles. Anti-FTZ-F1 cross reacts with BmFTZ-F1. These results indicate that BmFTZ-F1 is a B.mori homologue of FTZ-F1. The mobility of the factor-DNA complex formed in the silk gland extract changes depending on the developmental stages. Purification of BmFTZF1 to an almost homogeneous state reveals that the factor is a 73 kd protein. © 1990 Oxford University Press.

    DOI: 10.1093/nar/18.24.7229

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  • Transposable genetic element found in the 5′-flanking region of the fibroin H-chain gene in a genomic clone from the silkworm Bombyx mori

    Hitoshi Ueda, Shigeki Mizuno, Kensuke Shimura

    Journal of Molecular Biology   190 ( 3 )   319 - 327   1986.8

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    A transposable genetic element was found in the 5′-flanking region of the fibroin H-chain gene in one of the genomic clones from the silkworm Bombyx mori. This element, named K-1.4, is about 1.4 × 103 base-pairs long, contains an open reading frame of only 225 base-pairs and has inverted repeats of 12 base-pairs at both ends. Duplication of three base-pairs seems to have occurred when this element was integrated into the silkworm genome. About 15 copies of K-1.4 are present per haploid genome of various silkworm strains. Genomic loci of some of these elements are different among different strains or even among individual offspring of the same parents. K-1.4 is present also in the genome of Bombyx mandarina. The K-1.4-related sequences are present in some species belonging to the family Saturniidae. © 1986.

    DOI: 10.1016/0022-2836(86)90004-5

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Books

  • 分子間相互作用解析ハンドブック

    羊土社  2007 

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  • 転写因子・転写制御キーワードブック

    羊土社  2006 

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  • 生物化学実験法43,遺伝子発現研究法

    学会出版センター  2000 

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  • Genetical background of development and differentiation

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  • Molecular Mechanisms of Insect Metamorphosis and Diapause

    Induxtrial Publishing & Consulting,Inc 

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  • Gene group regulated by insect steroid hormons

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  • Genetical background of development and differentiation

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  • Molecular Mechanisms of Insect Metamorphosis and Diapause

    Induxtrial Publishing & Consulting,Inc 

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  • Gene group regulated by insect steroid hormons

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  • 発生・分化の遺伝子的背景

    東京大学出版会 

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  • 昆虫ステロイドホとモンで制御される遺伝子群

    学会出版センター 

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MISC

  • DNAマイクロアレイの昆虫学研究への利用と現状の展望

    神村 学, 上田 均

    植物防疫   60 (10) 483-485   2006

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  • 昆虫の脱皮と変態の分子機構

    上田 均

    化学と生物   44(8)525-531   2006

  • 発生過程におけるホルモン制御による遺伝子カスケイド

    上田 均

    蛋白質核酸酵素   48, 2254-2260   2003

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Presentations

  • New Side of Transvection: Transcriptional super activation is induced by chromosome pairing

    2019.12.6 

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  • Polished rice small peptide contributes to a biological timer system to determine pupation timing in Drosophila melanogaster

    2019.12.3 

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  • Function of Polished rice small peptide for a biological timer to determine prepupal period in Drosophila melanogaster International conference

    Koich Miyagawa, Hamdy Aly, Kazutaka Akagi, Yuji Kageyama, Hitoshi Ueda

    International Insect Hormone Workshop  2019.7.4 

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  • Function of Polished rice small peptide for the determination of prepupal period in Drosophila melanogaster

    Koich Miyagawa, Hamdy Aly, Kazutaka Akagi, Yuji Kageyama, Hitoshi Ueda

    2019.5.14 

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  • Degradation mechanism of labile transcriptional repressor Blimp-1 whose degradation speed affects prepupal period International conference

    Koich Miyagawa, Hamdy Aly, Kazutaka Akagi, Yuji Kageyama, Hitoshi Ueda

    The 60th Annual Drosophila Research Conference  2019.3.27 

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  • Mechanism of transcriptional activation by two different transcriptional activators through a single binding site

    Koichi Miyagawa, Hitoshi Ueda

    13th Japanese Drosophila Research Conference  2018.9.10 

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  • Why timer system to measure prepupal period is present in fat body?

    Haruka Nishida, Mayu Nakanishi, Hitoshi Ueda

    13th Japanese Drosophila Research Conference  2018.9.10 

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  • Effects of nutritional signal in the timer system to determine prepupal period in Drosophila melanogaster

    Hitoshi Ueda, Haruka Nishda, Mayu Nakanishi

    Joint Annual Meeting of JSDB 51st and JSCB 70th  2018.6.6 

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  • 昆虫の形づくりのしくみとその多様性

    上田 均

    日本遺伝学会第89大会公開講演会  2017.9.16 

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  • Degradation mechanism of a transcriptional repressor dBlimp-1, which regulate the pupation timing in Drosophila

    H. Aly, K. Akagi, H. Ueda

    The 3rd International Insect Hormone Workshop  2017.7.10 

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  • The advantages of repressor usage for a precise time measurement system in the development of Drosophila melanogaster International conference

    H. Nishida, K. Akagi, H. Ueda

    4th Asia-Pacific Drosophila Research Conference  2017.5.9 

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  • Mechanism of transcriptional activation by two different transcriptional activators through a single binding site International conference

    K. Miyagawa, H. Ueda

    58st Annual Drosophila Research Conference  2017.3.30 

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  • The mechanism of transcriptional activation by two different transcriptional activators through a single binding site International conference

    Koichi Miyagawa, Hitoshi Ueda

    58st Annual Drosophila Research Conference  2017.3.9 

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  • キイロショウジョウバエの転写因子βFTZ-F1による羽化タイミングの制御

    第39回日本分子生物学会年会  2017 

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  • Molecular players affecting the biological timer system to determine pupation timing in Drosophila

    第39回日本分子生物学会年会  2017 

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  • キイロショウジョウバエ幼虫期後期の栄養状態は蛹化タイミングを決定する生物タイマーに影響を与える

    昆虫ワークショップ2016  2016 

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  • ショウジョウバエの蛹化のタイミングを決めるタイマーシステムで転写抑制因子を使う利点

    昆虫ワークショップ2016  2016 

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  • Degradation mechanism of a transcriptional repressor dBlimp, which regulates pupation timing

    Japanese Drosophila Research Conference  2016 

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  • Expression pattern and function of transcriptional repressor Blimp-1 at late larval period

    Japanese Drosophila Research Conference  2016 

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  • Degradation mechanism of a transcriptional repressor dBlimp-1, which regulate the pupation timing in Drosophila

    第39回日本分子生物学会年会  2016 

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  • The advantages of repressor usage for a precise timer system

    第39回日本分子生物学会年会  2016 

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  • 新規の幼虫期ステージング方法を用いたエクダイソン関連遺伝子の発現解析

    第39回日本分子生物学会年会  2016 

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  • Timer system to determine pupation timing exists in fat body.

    Ecdysone Workshop, 55st Annual Drosophila Research Conference  2015 

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  • Control of developmental timing by transcription factors Blimp-1 and FTZ-F1 in fat body during prepupal period in Drosophila melanogaster.

    55st Annual Drosophila Research Conference  2015 

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  • Functional analysis of Blimp-1during pupal developmental stage in Drosophila.

    55st Annual Drosophila Research Conference  2015 

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  • Determination of pupation timing by biological timer in fat body during prepupal period in Drosophila melanogaster

    CDB Symposium 2015 “Time in Development”  2015 

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  • Biological timer in fat body determines pupation timing in Drosophila melanogaster.

    48th Meeting of the Japanese Society of Developmental Biologists  2015 

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  • Unstable character of transcriptional repressor Blimp-1 contributes to accurate time measuring system for pupation timing in Drosophila melanogaster.

    The 2nd International Insect Hormone Workshop  2015 

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  • Effect of culture temperature on the analysis process of timer system at the onset of metamorphosis in Drosophila melanogaster.

    第38回日本分子生物学会年会  2015 

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  • ブッポウソウの標識調査から見えてきたこと

    日本鳥学会2015年度大会  2015 

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  • Nutritional signal affects biological timer that determines prepupal period in Drosophila.

    第38回日本分子生物学会年会  2015 

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  • Regulation mechanism of the ftz-f1 gene and its function during the prepupal development in Drosophila

    日本分子生物学会年会  2011 

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  • ショウジョウバエEDG84A遺伝子の正確な発現調節には複数の核内レセプターが単一標的部位へ作用することが重要である

    日本分子生物学会年会  2011 

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  • FTZ‐F1 is required for eye development in Drosophila

    52st Annual Drosophila Research Conference  2011 

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  • Regulatory mechanisms of ecdysteroid-inducible Blimp-1 encoding a transcriptional repressor in the timer system to determine the prepupal period in Drosophila malanogaster

    44th Annual Meeting of the Japanese Society of Developmental Biologist  2011 

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  • ftz-f1 and dblimp-1 are required for the pupal development in Drosophila

    44th Annual Meeting of the Japanese Society of Developmental Biologist  2011 

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  • Prepupal period is determined by a common biological timer system in different Drosophila species

    43rd Annual Meeting for the Japanese Society of Developmental Biologists  2010 

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  • Mechanism to determine pupation timing during prepupal period in Drosophila

    18th International Ecdysone Workshop  2010 

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  • ショウジョウバエ近縁種間での前蛹期間の調節機構

    日本遺伝学会第82回大会  2010 

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  • ショウジョウバエ近縁種間において蛹化タイミングの違いを決定する分子機構

    第33回日本分子生物学会年会/第83回日本生化学会大会合同大会  2010 

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  • カイコの脱皮時におけるBlimp-1遺伝子の発現パターンの解析

    第33回日本分子生物学会年会/第83回日本生化学会大会合同大会  2010 

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  • Molecular analysis of factors interacting with Drosophila Blimp-1 to mediate transcriptional repression or activation of its target genes

    第33回日本分子生物学会年会/第83回日本生化学会大会合同大会  2010 

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  • Analysis of transcriptional repression and activation of the ftz-f1 gene by Blimp-1 using targeted insertion of the promoter-reporter fusion genes in Drosophila

    第33回日本分子生物学会年会/第83回日本生化学会大会合同大会  2010 

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  • A transcriptional repressor Blimp-1 works as an hourglass timer during Drosophila metamorphosis

    DECODE Winter Workshop 2010,  2010 

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  • A transcriptional repressor Blimp-1 works as an hourglass timer during metamorphosis

    51st Annual Drosophila Research Conference  2010 

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  • A transcriptional repressor Blimp-1 works as an hourglass timer during Drosophila metamorphosis -Determination mechanism of pupation timing by transcription factors-

    Ecdysone workshop, 51st Annual Drosophila Research Conference  2010 

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  • ショウジョウバエの蛹化のタイミングを決める生物タイマーにおける転写抑制因子Blimp-1の発現制御の重要性

    遺伝情報DECODE冬のワークショップ(転写研究会共催)  2009 

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  • The importance of controlled expression of the transcription factor Blimp-1 in a biological timer system to determine pupation timing in Drosophila

    学内COE「複眼的観点を養うバイオサイエンス教育」平成20年次成果発表会  2009 

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  • A transcriptional repressor Blimp-1 works as an hourglass in a biological timer system to determine developmental timing at the onset of metamorphosis in Drosophila

    First International Conference of Biological Sciences  2009 

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  • The importance of controlled expression of the transcriptional repressor Blimp-1 in a biological timer system to determine pupation timing in Drosophila melanogaster

    50th Annual Drosophila Research Conference  2009 

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  • Mechanism of space-specific expression of the EDG84A gene in Drosophila melanogaster

    The 3rd Insect Genomes Research Meeting  2009 

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  • Blimp-1はショウジョウバエの蛹化タイミングを決めるhourglass timerである

    昆虫ワークショップ09  2009 

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  • カイコの脱皮時におけるBlimp-1 遺伝子の発現パターンの解析

    昆虫ワークショップ09  2009 

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  • Analysis of factors interacting with Drosophila Blimp-1, a transcriptional repressor that gives a potential to activate its target gene ftz-f1.

    第32回 日本分子生物学会年会  2009 

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  • A transcriptional repressor Blimp-1 works as an hourglass timer to determine pupation timing in Drosophila

    第32回 日本分子生物学会年会  2009 

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  • Abdel-Rahman Sultan, Moustafa Sarhan, Kazutaka Akagi and Hitoshi Ueda

    第32回 日本分子生物学会年会  2009 

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  • Temporal and spatial expression pattern of ecdysone inducible transcription factor Blimp-1 and FTZ-F1, components of a biological timer system to determine pupation timing

    第32回 日本分子生物学会年会  2009 

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  • The importance of controlled expression of the transcription factor Blimp-1 in a biological timer system to determine pupation timing in Drosophila

    42nd Annual Meeting for the Japanese Society of Developmental Biologists  2009 

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  • Regulatory mechanism of a ecdysone primary-response gene Blimp-1, it differs from well-known early genes

    The 9th Japanese Drosophila Research Conference  2009 

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  • A transcriptional repressor Blimp-1 works as an hourglass in a biological timer system to determine pupation timing in Drosophila

    The 9th Japanese Drosophila Research Conference  2009 

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  • Spatial specific expression pattern of ecdysone inducible transcription factor Blimp-1, a factor determines pupation timing

    The 9th Japanese Drosophila Research Conference  2009 

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  • Transcriptional repressor Blimp-1 works as an hourglass in the biological timer system to determine pupation timing in Drosophila

    日本遺伝学会 第81回大会  2009 

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  • Screening for mutants that have different prepupal periods in Drosophila melanogaster

    第32回 日本分子生物学会年会  2009 

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  • Stability of transcription factor Blimp-1 plays a crucial role in determining developmental timing at the onset of metamorphosis in Drosophila

    Naito Conference “Nuclear Dynamics and RNA II”  2008 

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  • ショウジョウバエの蛹化のタイミングを決める生物タイマーにおける転写抑制因子Blimp-1の発現制御の重要性

    第31回日本分子生物学会年会/第81回日本生化学会大会合同大会  2008 

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  • ショウジョウバエEDG84A遺伝子の組織特異的発現の制御機構の解析

    第31回日本分子生物学会年会/第81回日本生化学会大会合同大会  2008 

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  • ショウジョウバエの蛹化のタイミングを決める生物タイマーにおける転写抑制因子Blimp-1の発現制御の重要性

    遺伝情報DECODE・冬のワークショップ/転写研究会  2008 

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  • Regulation mechanism of the Blimp-1 gene which plays an important role to determine pupation timing

    Ecdysone workshop  2008 

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  • Regulatory mechanism of temporal expression of Blimp-1, an important factor for determination of pupation timing

    Annual Drosophila Research Conference  2008 

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  • dBlimp-1, a developmental timer protein controls pupation timing through its stability

    Annual Drosophila Research Conference  2008 

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  • Regulation of developmental timing by transcription factors in Drosophila melanogaster

    The First Egypt-Japan International Symposium on Science and Technology  2008 

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  • Characterization and Expression of the Insect-Specific Scorpion Neurotoxins for Pest Control

    The First Egypt-Japan International Symposium on Science and Technology  2008 

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  • Roles of dBlimp-1and βFTZ-F1 in the process of determination of pupation timing in D. melanogaster

    Annual Drosophila Research Conference Ecdysone Workshop  2007 

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  • Domain analysis of the dBlimp-1, an ecdysone inducible and labile transcription factor in Drosophila melanogaster

    Annual Drosophila Research Conference  2007 

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  • Drosophila Blimp-1 is a transient transcription repressor that controls timing of the ecdysone-induced developmental pathway

    Annual Drosophila Research Conference  2007 

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  • Regulation of developmental timing by transcription factors in Drosophila

    廣瀬進教授退職記念シンポジウム「遺伝子発現と発生」  2007 

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  • The molecular mechanism that determines pupation timing in D. melanogaster

    The 7th MBSJ Spring Symposium  2007 

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  • ショウジョウバエの不安定な転写因子 dBlimp-1 が変態過程でのタイミングを決める機構

    遺伝情報DECODE/転写研究会・冬のワークショップ  2007 

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  • 染色体間相互作用による遺伝子発現制御

    遺伝情報DECODE/転写研究会・冬のワークショップ  2007 

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  • Molecular dissection of a developmental timer protein, dBlimp-1 in Drosophila

    International Symposium “Comprehensive understanding of diverse biological timing mechanism”  2007 

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  • Molecular Mechanism to determine pupation timing in Drosophila melanogaster

    Hogness symposium  2007 

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  • Regulatory mechanism to determine pupation timing by transcription factors in Drosophila melanogaster

    日本分子生物学会年会  2007 

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  • Mechanism for transcriptional regulation of the Blimp-1 gene is different from that of the well-known early gene pattern

    日本分子生物学会年会  2007 

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  • Regulatory mechanism of Blimp-1 induction that differs from the well-known early gene pattern

    Japanese Drosophila Research Conference  2007 

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  • Control of the pupation timing: role of dBlimp-1 and βFTZ-F1 in the process of determination of pupation timing

    Japanese Drosophila Research Conference  2007 

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  • ショウジョウバエの不安定な転写因子 Blimp-1 が変態過程でのタイミングを決める機構

    日本遺伝学会第79回大会  2007 

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  • Regulation of developmental timing by transcription factors in Drosophila

    International Symposium “Gene Expression Control and Genome Evolution”  2007 

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  • Molecular mechanism to determine pupation timing in Drosophila melanogaster

    International Symposium “Comprehensive understanding of diverse biological timing mechanism”  2007 

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  • Regulatory mechanism of temporal expression of Drosophila Blimp-1, an important factor for determination of pupation timing

    International Symposium “Comprehensive understanding of diverse biological timing mechanism”  2007 

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  • Midline governs axon pathfinding by coordinating expression of multiple guidance molecules

    日本分子生物学会年会  2007 

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  • 転写活性化のポテンシャルを与える転写活性化因子p170の解析

    遺伝情報DECODE冬のワークショップ  2006 

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  • 昆虫の発生過程でタイミングを制御する機構

    公開シンポジウム、生命現象の多様なタイミング機構の総合的理解:時間を基軸とした生命科学の構築  2006 

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  • p170 is a labile transcription factor induced by ecdysone

    Ecdysone workshop  2006 

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  • p170 is a labile transcription factor induced by ecdysone in Drosophila melanogastor

    20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress  2006 

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  • A Molecular Approach to Intraspecific Variation in Scorpio maurus palmatus Venom Collected from Different Geographical Regions

    20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress  2006 

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  • 転写因子FTZ-F1とp170のキイロショウジョウバエの蛹化時期決定における役割

    昆虫ワークショップ08  2006 

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  • ショウジョウバエftz-f1遺伝子の転写を制御する因子p170の発現制御機構の解析

    昆虫ワークショップ08  2006 

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  • ショウジョウバエの脱皮・変態に関わるftz-f1遺伝子の転写制御因子p170の機能解析

    日本分子生物学会年会  2005 

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  • Domain analysis of a unique transcription factor p170

    日本分子生物学会年会  2005 

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  • p170 a novel Drosophila transcription factor that potentiates expression of the FTZ-F1 gene during high ecdysteroid periods.

    JBSバイオシンポジウム&第4回転写研究会  2005 

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  • Screening (project) of transposon insertion mutants in the silkworn, Bombyx mori

    CDB Symposium "Origin and Development of the Vertebrate Traits"  2005 

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  • 昆虫の脱皮・変態のタイミングを制御する機構

    「生命現象の多様なタイミング機構の総合的理解-時間を基軸とした生命科学の構築」第1回研究会  2005 

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  • ショウジョウバエの神経発生におけるMidlineの機能解析

    日本遺伝学会年会  2005 

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  • 転写活性化のポテンシャルを与える転写抑制因子p170

    第3回転写研究会  2004 

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  • Drosophila transcriptional repressor that potentiates high level expression of the bFTZ-F1 gene during high ecdysteroid periods

    Ecdysone Workshop  2004 

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  • p170, A Novel Drosophila Transcription Factor that Regulates Expression of the FTZ-F1 Gene During High Ecdysteroid Periods

    Annual Drosophila Research Conference  2004 

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  • エクダイソンパルス後に誘導される転写因子FTZ-F1遺伝子の転写抑制因子p170

    昆虫ワークショップ04  2004 

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  • ショウジョウバエFTZ-F1遺伝子の転写調節領域に作用する因子p170の解析

    27回日本分子生物学会年会  2004 

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  • エピジェネティックサイレンシングの防波堤として働くクロマチンリモデリング

    第26回日本分子生物学会年会シンポジウム「エピジェネティクスの新展開 (1)」  2003 

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  • ショウジョウバエFTZ-F1遺伝子に発現のポテンシャルを与える因子

    第2回転写研究会  2003 

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  • Activation mechanism of Edg genes during early metamorphic period in Drosophila melanogaster

    Annual Drosophila Research Conference  2003 

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  • p170 a novel Drosophila transcriptional activator for the FTZ-F1 gene

    Annual Drosophila Research Conference, Workshop, Ecdysone action  2003 

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  • ショウジョウバエFTZ-F1遺伝子の転写調節因子p170

    第6回ショウジョウバエ研究会  2003 

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  • Tボックスタンパク質をコードしているショウジョウバエdtbは中枢神経系とモーターニューロンの軸索誘導に必要である

    第6回ショウジョウバエ研究会  2003 

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  • ショウジョウバエFTZ-F1の転写調節領域に作用する因子p170の解析

    第26回日本分子生物学会  2003 

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  • ショウジョウバエの神経発生におけるDtbの機能解析

    第26回日本分子生物学会  2003 

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  • p170 a novel Drosophila transcription factor that potentiates expression of the FTZ-F1 gene during high ecdysteoid periods

    Cold Spring Harbor Meeting on Mechanisms of Eukaryotic Transcription, Cold Spring Harbor  2003 

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  • Drosophila GAGA factor recruits FACT to multiple specific loci in the genome and forms a boundary against silencing.

    Cold Spring Harbor Meeting on Mechanisms of Eukaryotic Transcription  2003 

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  • Epigenetic gene expression through GAGA factor-dependent chromatin remodeling.

    7th Asian Conference on Transcription  2002 

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  • Mechanism of space specific gene expression in response to ecdysteroids-Transcriptional regulation of FTZ-F1 target gene EDG84A.

    43rd Annual Drosophila Research Conference  2002 

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  • Mechanism of space specific gene expression in response to ecdysteroids-Transcriptional regulation of FTZ-F1 target gene EDG84A

    43rd Annual Drosophila Research Conference  2002 

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  • MBF1 serves as atranscriptional coactivator of Tracheae defective/Apontic during development of the trachea and central nervous system in Drosophila melanogaster.

    43rd Annual Drosophila Research Conference  2002 

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  • Functional analysis of Drosophila TDF during the neuronal development.

    分子生物学会年会  2002 

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  • Mechanism of transcriptional regulation of the FTZ-F1 gene in Drosophila melanogaster.

    分子生物学会年会  2002 

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  • Candidates for regulator of space-specific expression of FTZ-F1-target gene EDG84A in Drosophila.

    日本分子生物学会年会  2002 

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  • Transcriptional activation mechanism by FTZ-F1

    生化学会  2002 

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  • Chromosome pairing-dependent gene regulation in Drosophila. 25th Annual

    Meeting of Molecular Biology Society of Japan  2002 

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  • ショウジョウバエ核内レセプターFTZ-F1の機能

    細胞生物学会  2000 

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  • Basic Biology A1 (2020academic year) 1st semester  - 金3,金4

  • Basic Biology A2 (2020academic year) Second semester  - 金3,金4

  • Seminar in Developmental Biology (2020academic year) Year-round  - その他

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  • Developmental Biology IIB (2020academic year) Second semester  - 月1,月2

  • Developmental Genetics (2020academic year) Late  - その他

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