研究者詳細 - 沈　建仁

PsbA3-D1 タンパク質を発現する光化学系II 複合体の結晶構造

その他リンク： https://jlc.jst.go.jp/DN/JLC/20021928091?from=CiNii

SACLAが解き明かす光合成の仕組み

沈建仁

月刊 OPTRONICS 409 31 - 35 2016年

光合成水分解触媒のモデル化合物の合成ー人工光合成の実現に向けて

沈建仁

化学 72 46 - 50 2016年

Damage-free structure of photosystem II determined by femtosecond X-ray free electron laser

Journal of the Japanese Crystallography Society 58 ( 3 ) 126 - 132 2016年

Mechanism of photosynthetic water-splitting revealed by SACLA

OPTRONICS 409 31 - 35 2016年

X線自由電子レーザーで決定した光化学系II複合体の無損傷結晶構造と水分解反応機構

放射光学会誌 28 177 - 181 2015年

Damage-free structure of photosystem determined by X-ray free electron laser and the mechanism of water-splitting

28 177 - 181 2015年

鵜飼奈津美, 菅倫寛, 杉浦美羽

光合成研究 25 ( 1 ) 22 - 27 2015年

高等植物の光化学系I-光捕集アンテナI超複合体におけるエネルギー伝達経路の構造基盤

ライフサイエンス新着論文レビュー 2015年

Protein 3D structures -- Photosystem II complex

55 226 2015年

Crystal structure of Photosystem II with the D1 protein encoded by the psbA3 gene

Photosynthesis Research 25 22 - 27 2015年

タンパク質立体構造散歩光化学系II複合体

生物物理 55 226 2015年

Large-scale QM/MM calculations of hydrogen bonding networks for proton transfer and water inlet channels for water oxidation—Theoretical system models of the oxygen-evolving complex of Photosystem II

Mitsuo Shoji, Hiroshi Isobe, Shusuke Yamanaka, Yasufumi Umena, Keisuke Kawakami, Nobuo Kamiya, Jian-Ren Shen, Takahito Nakajima, Kizashi Yamaguchi

Advances in Quantum Chemistry 70 325 - 413 2015年

The mystery of photosynthesis revealed by crystal structural analysis

7 22 - 25 2014年

結晶構造解析が解き明かす光合成の謎

ミルシル 7 22 - 25 2014年

フェムト秒X線レーザーにより明らかにされた1.95Å分解能における光化学系II複合体の天然状態の構造

ライフサイエンス新着論文レビュー 2014年

光化学系II複合体の高分解能構造と水分解反応の機構

実験医学増刊号「構造生命科学で何がわかるのか，何ができるのか」 32 142 - 147 2014年

Native structure of photosystem II at 1.95 A resolution revealed by femtosecond X-ray laser

2014年

Crystal structure of Extrinsic protein in photosystem II 査読

R. Nagao, M. Suga, A. Niikura, A. Okumura, F.H.M. Koua, T. Suzuki, T. Tomo, I. Enami, J.R. Shen

2013年9月

26aXC-1 植物型光合成反応中心の光捕集過程 : 構造情報に基づく理解2(26aXC 化学物理1,領域12(ソフトマター物理,化学物理,生物物理))

柴田穣, 西俊輔, 川上恵典, 沈建仁, Renger Thomas

日本物理学会講演概要集 68 ( 1 ) 414 - 414 2013年3月

光合成の水分解・酸素発生機構の解明に向けて

神谷信夫, 川上恵典, 梅名泰史, 沈建仁

放射光学会誌 26 3 - 10 2013年

High resolution structure of photosystem II and the mechanism of water-splitting

Jian-Ren Shen, Yasufumi Umena, Keisuke Kawakami, Nobuo Kamiya

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 1817 S2 - S3 2012年10月

Mechanism of photosynthetic water-splitting and oxygen evolution based on high resolution structure of photosystem II

Jian-Ren Shen

67 15 - 19 2012年

光合成最大の謎を解明

沈建仁

教科研究理科 195 12 - 16 2012年

高分解能結晶構造に基づく光合成水分解・酸素発生の分子機構

沈建仁

酵素工学ニュース 67 15 - 19 2012年

高分解能構造から見えてきた光合成水分解の仕組み

沈建仁, 梅名泰史, 川上恵典, 神谷信夫

生物物理 52 ( 3 ) 140 - 143 2012年

記述言語：日本語出版者・発行元：The Biophysical Society of Japan General Incorporated Association

Photosystem II (PSII) is a membrane-protein complex consisting of 17 trans-membrane subunits and 3 peripheral, extrinsic subunits with a total molecular mass of 350 kDa for a monomer. PSII performs a series of light-induced electron transfer reactions, leading to the conversion of light energy into biologically useful chemical energy, coupled with this is the splitting of water and generation of molecular oxygen. Both the chemical energy converted and molecular oxygen generated by PSII are indispensible for sustaining life on the earth; thus PSII is an extremely important protein complex. We have succeeded in crystallizing the PSII complex at a resolution of 1.9 Å, and analyzed its structure. Here we describe the structure of PSII, in particular the Mn₄CaO₅-cluster, which is the catalytic center of water-splitting, analyzed at the atomic resolution. Based on these, we discuss the possible mechanisms of light-induced water-splitting and its implications in artificial photosynthesis.<br>

DOI： 10.2142/biophys.52.140

その他リンク： https://jlc.jst.go.jp/DN/JALC/10000488562?from=CiNii

Running on sun

M. M, Najafpour, J. Barber, J-R. Shen, G. F. Moore, Govindjee

Chemistry World 2012 ( November ) 43 2012年

高分解能構造から見えてきた光合成水分解の仕組み

沈健仁, 梅名泰史, 川上恵典, 神谷信夫

生物物理 52 ( 3 ) 140 - 143 2012年

記述言語：日本語出版者・発行元：The Biophysical Society of Japan General Incorporated Association

DOI： 10.2142/biophys.52.140

光化学系2複合体の構造と機能 (生命秩序を担う生体超分子) -- (超分子による生体エネルギー・物質変換とその制御)

その他リンク： https://jlc.jst.go.jp/DN/JALC/10000488562?from=CiNii

Elucidating the largest mystery of photosynthesis

Jian-Ren Shen

195 12 - 16 2012年

光化学系IIにおける酸素発生中心Mnクラスターの多周波EPR研究

松岡秀人, SHEN Jian-Ren, 伊東信哉, 古川貢, 中村敏和, 山内清語

電子スピンサイエンス学会年会講演要旨集 51st 2012年

25pGP-7 光化学系IIマンガンクラスターの電子構造の密度汎関数理論による研究(25pGP 領域12シンポジウム:光化学系IIにおける酸素発生中心,領域12(ソフトマター物理,化学物理,生物物理))

山中秀介, 神田慧太, 梅名泰史, 川上恵典, 沈建仁, 神谷信夫, 奥村光隆, 中村春木, 山口兆

日本物理学会講演概要集 66 ( 1 ) 378 - 378 2011年3月

25pGP-2 光合成酸素発生を支える光化学系IIの原子構造(25pGP 領域12シンポジウム:光化学系IIにおける酸素発生中心,領域12(ソフトマター物理,化学物理,生物物理))

沈建仁, 梅名泰史, 川上恵典, 神谷信夫

日本物理学会講演概要集 66 ( 1 ) 376 - 376 2011年3月

27pHC-12 結晶構造情報に基づくシミュレーションによる光化学系II反応中心の時間分解蛍光スペクトルの解析(27pHC 非線形光学/超高速現象,領域5(光物性))

柴田穣, 西俊輔, 野地智康, 川上恵典, 沈建仁, Renaer Thomas

日本物理学会講演概要集 66 ( 1 ) 794 - 794 2011年3月

光合成水分解・酸素発生を可能にする光化学系IIの原子構造

沈建仁

光合成研究 21 72 - 79 2011年

Location and function of chloride ions in oxygen-evolving photosystem II revealed by X-Ray crystallography

Jian-Ren Shen

SPring-8 Research Frontiers 2009 22 - 23 2010年

系II複合体の精製法

沈建仁, 榎並勲

低温科学 67 275 - 283 2009年

電子伝達活性

沈建仁

低温科学 67 551 - 560 2009年

光化学系IIの例から見る膜タンパク質複合体の結晶化と構造解析

沈建仁, 川上恵典

光合成研究 19 19 - 25 2009年

単結晶光化学系II複合体の高周波CW/パルスESR研究

松岡秀人, SHEN Jian-Ren, 西山圭, 大庭裕範, 山内清語, 古川貢, 中村敏和, 河盛阿佐子

電子スピンサイエンス学会年会講演要旨集 48th 2009年

A brief introduction of Kimiyuki Satoh TRIBUTE

Isao Enami, Jian-Ren Shen

PHOTOSYNTHESIS RESEARCH 98 ( 1-3 ) 7 - 11 2008年10月

Purification and crystallization of photosystem II dimmer complex

Hideyuki Adachi, Isao Enami, Takahiro Henmi, Nobuo Kamiya, Jian-Ren Shen

Photosynthesis. Energy from the Sun 1 351 - 356 2008年

光化学系II反応中心の多周波EPR研究

松岡秀人, SHEN Jian-Ren, 西山圭, 古川貢, 中村敏和, 山内清語, 河盛阿佐子

電子スピンサイエンス学会年会講演要旨集 47th 2008年

Purification and crystallization of photosystem II complex from a red alga Cyanidium Caldarium.

Hideyuki Adachi, Isao Enami, Takahiro Henmi, Nobuo Kamiya, Jian-Ren Shen

PLANT AND CELL PHYSIOLOGY 48 S98 - S98 2007年

Crystal structure analysis of a mutant photosystemII complex lacking PsbM from Thermosynechococcus vulcanus.

Yosuke Kawabata, Takahiro Henmi, Masako Iwai, Takashi Suemasu, Keisuke Kawakami, Chika Aoyama, Masahiko Ikeuchi, Nobuo Kamiya, Jian-Ren Shen

PLANT AND CELL PHYSIOLOGY 48 S97 - S97 2007年

Roles of lipids in photosystem II from cyanobacteria

J Leng, Sakurai, I, H Wada, Shen, JR

PLANT AND CELL PHYSIOLOGY 47 S162 - S162 2006年

Current status and prospects of PSII structure analysis

Shen, JR, H Naitow, M Furuse, S Saijo, A Ohkuma, K Kawakami, T Henmi, N Kamiya

PLANT AND CELL PHYSIOLOGY 47 S9 - S9 2006年

神谷信夫, 沈建仁

蛋白質核酸酵素 50 ( 10 ) 1174 - 1179 2005年8月

光化学系II複合体の構造と機能

神谷信夫, 沈建仁

蛋白質核酸酵素 50, 1174-1179. 2005年

W-band EPR studies of Mn-cluster in the S-0 and S-2 states of Cyanobacterial single crystals.

A Kawamori, Shen, JR, K Furukawa, E Matsuoka, T Kato

PLANT AND CELL PHYSIOLOGY 45 S81 - S81 2004年

Thermodynamic and kinetic analysis of unfolding of P23k protein isolated from spinach photosystem II

CY Tan, CH Xu, Shen, JR, S Sukuma, Y Yamamoto, C Balny, KC Ruan

ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35 ( 7 ) 677 - 681 2003年7月

Crystal structure analysis of photosystem II from Thermosynechococcus vulcanus

Kamiya N, Shen J.-R

PS2001 Proceedings, 12th International Congress on Photosynthesis 2002年

Cross-reconstitution of cytochrome c550 and 12 kDa protein between cyanobacterial and red algal PSII.

Enami I, Iwai M, Akiyama A, Nakata Y, Ohta H, Shen J.-R

PS2001 Proceedings, 12th International Congress on Photosynthesis 2002年

Distribution of extrinsic proteins among various organisms as an index of evolution of oxygen-evolving PSII.

Nakata Y, Satoh A, Nakamura K, Okumura A, Ohta H, Shen J.-R, Enami I

PS2001 Proceedings, 12th International Congress on Photosynthesis 2002年

Reconstitution of the 23 and 17 kDa proteins with spinach PSII which had been exchanged for the 33 kDa protein from different plant species.

Suzuki T, Akiyama A, Iwai M, Tohri A, Tomo T, Ohta H, Shen J.-R, Enami I

PS2001 Proceedings, 12th International Congress on Photosynthesis 2002年

Evolution of oxygen-evolving PSII in view of extrinsic proteins among various organisms. II

Y Nakada, H Ohta, Shen, JR, Enami, I

PLANT AND CELL PHYSIOLOGY 43 S63 - S63 2002年

Functional Analysis of psbV and a Novel c-type Cytochrome Gene psbV2 of the Thermophilic Cyanobacterium Thermosynechococcus elongatus Strain BP-1

Katoh Hiroshi, Itoh Suwako, Shen Jian-Ren, IKEUCHI Masahiko

Plant and cell physiology 42 ( 6 ) 599 - 607 2001年6月

紅藻チアニジウムの酸素発生標品に存在する表在性20kDa蛋白のクローニングと発現

太田尚孝, 上野匡司, 沈建仁, 加茂政晴, 榎並勲

日本植物生理学会年会要旨集 40th 218 2000年3月

Crystallization and the crystal properties of the oxygen-evolving photosystem II from Synechococcus vulcanus.

Shen J.-R, Kamiya N

Biochemistry 2000年

27aN-1 光捕集クロロフィルa/b蛋白質複合体が正八面体結晶中で形成する高次構造

日野智也, 金森英司, 五藤俊昭, 竹田一旗, 佐藤英憲, 沈建仁, 井上頼直, 神山勉

日本物理学会講演概要集 54 ( 2 ) 318 - 318 1999年9月

CLONING OF THE GENES FOR CYTOCHROME C550 AND A C550-LIKE PROTEIN FROM THE THERMOPHILIC CYANOBACTERIUM Synechococcus elongatus.

KATOH Hiroshi, ITOH Suwako, SHEN Jian-Ren, IKEUCHI Masahiko

Plant and cell physiology 40 s34 - s34 1999年3月

COMPARISON OF BINDING AND FUNCTIONAL PROPERTIES OF Cyt c550 FROM CYANOBCTERIAL PSII WITH THOSE OF Cyt c550 FROM RED ALGAL PSII

IWAI Masako, AKIYAMA Ai, OHTA Hisataka, SHEN Jian-Ren, ENAMI Isao

Plant and cell physiology 40 s33 - s33 1999年3月

CLONING OF THE GENE ENCODING THE PSII EXTRINSIC 33 kDa PROTEIN FROM A RED ALGA

OKUYAMA Satoshi, OHTA Hisataka, SHEN Jian-Ren, ENAMI Isao

Plant and cell physiology 40 s30 - s30 1999年3月

THE STRUCTURE OF PHOTOSYSTEM II IN THYLAKOID MEMBRANES IMPLICATED FROM THE FUNCTIONAL DIFFERENCES OF ISOLATED PSII MONOMER AND DIMER

SHEN Jian-Ren

Plant and cell physiology 40 s33 - s33 1999年3月

COMPARISON OF PSII CRYSTALS BETWEEN HIGHER PLANTS AND CYANOBACTERIA

SHEN Jian-Ren, AOYAMA Hiroshi, INOUE Yorinao, NOBUO Kamiya

Plant and cell physiology 40 s33 - s33 1999年3月

CROSS-RECONSTITUTION OF FOUR EXTRINSIC PROTEINS FROM A RED ALGA WITH HIGHER PLANT AND CYANOBACTERIAL PSII

Isao Enami, Shizue Yoshihara, Hisataka Ohta, Jian-Ren Shen

Photosynthesis:Mechanisms and Effects Vol 2 1435 - 1438 1999年

好熱性シアノバクテリアSynechococcuselongatusのチトクロムc550およびc550類似タンパク質の遺伝子のクローニング

加藤浩, 伊藤須和子, 沈建仁, 池内昌彦

日本植物生理学会年会要旨集 39th 1999年

光捕集クロロフィル蛋白複合体のX線構造解析

日野智也, 佐藤英憲, 岩永誠之, 沈建仁, 井上頼直, 神山勉

生物物理 38 ( 2 ) S15 1998年9月

高等植物, 紅藻, ラン色細菌の系II表在性蛋白のCross-Reconstitution

榎並勲, 吉原静恵, 岩井雅子, 秋山愛, 太田尚孝, 沈建仁

日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan 62 118 - 118 1998年9月

紅藻チアニジウムの酸素発生標品に存在する表在性蛋白チトクロームc‐550のクローニングと発現

太田尚孝, 加藤宝, 奥村彰槻, 沈建仁, 加茂政晴, 榎並勲

日本植物生理学会年会要旨集 38th(1998) 75 1998年5月

CROSS-RECONSTITUTION OF FOUR EXTRINSIC PROTEINS FROMA RED ALGA TO HIGHER PLANT PSII

YOSHIHARA Shizue, OHTA Hisataka, SHEN Jian-Ren, ENAMI Isao

Plant and cell physiology 39 S15 - S15 1998年5月

CONSTRUCTION OF MONOCLONAL ANTIBODIES AGAINST PSII REACTION CENTER PROTEINS AND THE USE OF THEM TO ANALYZE THE TOPOLOGY OF PSII REACTION CENTER

TOMO Tatsuya, SHEN Jian-Ren, OGAWA Kenji, INOUE Yorinao

Plant and cell physiology 39 S12 - S12 1998年5月

31a-YB-2 膜蛋白質の球殻構造体

日野智也, 竹田一旗, 佐藤英憲, 櫻井郁子, 沈建仁, 井上頼直, 岡田哲二, 神山勉

日本物理学会講演概要集 53 ( 1 ) 731 - 731 1998年3月

LHC-IIの結晶とbRの結晶の構造上の類似性

日野智也, 竹田一旗, 沈建仁, 井上頼直, 櫻井郁子, 岡田哲二, 神山勉

生物物理 37 S126 1997年10月

CLONING AND EXPRESSION OF A cDNA ENCODING THE EXTRINSIC 12 KDA PROTEIN INVOLVED IN OXYGEN-EVOLVING PS II COMPLEX FROM A RED ALGA Cyanidium caldarium

OKUMURA Akinori, OHTA Hisataka, SHEN Jian-Ren, KAMO Masaharu, ENAMI Isao

Plant and cell physiology 38 s25 1997年3月

CRYSTALLIZATION OF OXYGEN-EVOLVING PSII COMPLEX : EFFECTS OF DETERGENTS

SHEN Jian-Ren, AOYAMA Hiroshi, INOUE Yorinao, KAMIYA Nobuo

Plant and cell physiology 38 s22 1997年3月

IDENTIFICATION OF LYS RESIDUES ON THE EXTRINSIC 33KDA PROTEIN INVOLVED IN ELECTROSTATIC INTERACTION WITH PSII COMPLEX BY CHEMICAL MODIFICATION WITH NSP

KAMEI Ayako, SHEN Jian-Ren, OHTA Hisataka, DOMAE Naoshi, TAKIO Koji, INOUE Yorinao, ENAMI Isao

Plant and cell physiology 38 s25 1997年3月

RECONSTITUTION OF THREE EXTRINSIC PROTEINS, THE 20KDA-, 12KDA- PROTEINS AND CYTC-550, IN OXYGEN-EVOLVING PSII COMPLEX FROM A RED ALGA, Cyanidium caldarium

KIKUCHI Sanae, FUKUDA Tetuya, OHTA Hisataka, SHEN Jian-Ren, ENAMI Isao

Plant and cell physiology 38 s25 1997年3月

Analysis of the psbU gene encoding the 12-kDa extrinsic protein of Photosystem II and studies on its role by deletion mutagenesis in Synechocystis sp. PCC 6803

SHEN J.-R.

J. Biol. Chem. 272 17821 - 17826 1997年

CLONING AND DIRECTED MUTAGENESIS OF THE GENE CODING FOR A 12 kDa PHOTOSYSTEM II EXTRINSIC PROTEIN FROM Synechocystis sp. PCC 6803

SHEN Jian-Ren, INOUE Yorinao

Plant and cell physiology 37 36 - 36 1996年3月

Chemical crosslinking studies ofextrinsic proteins in cyanobacterial photosystem II

HAN Kab-Cho, SHEN Jian-Ren, IKEUCHI Masahiko, INOUE Yorinao

Plant and cell physiology 36 S92 1995年3月

The role of cytochrome c-550 as studied through reverse genetics and mutant characterization in Synechocystis sp. PCC 6803

SHEN J. R.

J. Biol. Chem. 270 6901 - 6907 1995年

Binding and functional properties of two new extrinsic components, cytchrome c-550 and 12 kDa protein, in cyanobacterial photosystem II

SHEN J. -R.

Biochemistry 32 1925 - 1932 1993年

Cellular localization of cytochrome c550. Its specific_association with cyanobacterial photosystem II

SHEN J. -R.

J. Biol. Chem. 268 20408 - 20413 1993年

Stoichiometric association of extrinsic cytochrome c_<550> and 12 kDa protein with a highly purified oxygen-evolving photosystem II core complex from Synechococcus vulcanus

SHEN J. -R.

FEBS Lett. 301 145 - 149 1992年

Low pH-Induced Dissociation of Three Extrinsic Proteins from O_2-Evolving Photosystem II :

Shen Jian-Ren, Inoue Yorinao

Plant and cell physiology 32 ( 3 ) 453 - 457 1991年

記述言語：英語出版者・発行元：Japanese Society of Plant Physiologists

Three extrinsic proteins involved in oxygen evolution reversibly dissociated from Photo-system II (PS II) membranes at acidic pHS showing distinctly different pH dependencies. The pHS for half dissociation of 17, 23 and 33 kDa extrinsic proteins were determined to be 5.0, 4.1 and 3.6, respectively. The half dissociation pHS Of 17 and 23 kDa proteins were much lower than their respective isoelectric points, while that for 33 kDa protein was close to its isoelectric point. It was suggested that protonation of the negatively charged binding domain on membrane proteins causes dissociation of the former two extrinsic proteins, whereas protonation of the extrinsic protein itself is responsible for the dissociation of 33 kDa protein. Based on these, features of low pH-induced dissociation of extrinsic proteins and Mn from PS II were discussed.

Inactivation and Calcium-Dependent Reactivation of Oxygen Evolution in Photosystem II Preparations Treated at pH 3.0 or with High Concentrations of NaCl :

その他リンク： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=181397

Shen Jian-Ren, Katoh Sakae

Plant and cell physiology 32 ( 3 ) 439 - 446 1991年

記述言語：英語出版者・発行元：Japanese Society of Plant Physiologists

A brief treatment at pH 3.0 of Photosystem II (PS II) membranes containing two bound Ca^<2+> from rice resulted in strong suppression of oxygen evolution concomiiant with extraction of one Ca^<2+> and the lost activity was restored on addition of 50 mM Ca^<2+>. However, inactivation of oxygen evolution by low pH-treatment of oxygen-evolving PS II complexes containing only one Ca^<2+> from a rice chlorophyll b-deficient mutant was not associated with extraction of the bound Ca^<2+>, although oxygen evolution was markedly enhanced by the addition of Ca^<2+> to the treated complexes. Thus, the acid-inactivation of oxygen evolution cannot be related to extraction of Ca^<2+>. On the other hand, low pH-treatment was found to share the following common features with NaCl-treatment which also causes a Ca^<2+>-reversible inactivation of oxygen evolution. (1) Exposure of PS II membranes to pH 3.0 resulted in solubilization of the 23 and 17 kDa extrinsic proteins, although the released proteins rebound to the membranes when pH was raised to 6.5. (2) There was an apparent heterogeneity in the binding affinity of Ca^<2+> effective in restoration of the oxygen-evolving activity. (3) Low pH-treated preparations required a higher concentration of Ca^<2+> for the maximum reactivation of oxygen evolution than did NaCl-washed preparations. This was also the case with Sr^<2+>, which stimulated oxygen evolution of both low pH-treated and NaCl-washed PS II membranes to smaller extents. When the extrinsic 23 and 17 kDa proteins had been removed, however, Ca^<2+> concentration dependence of oxygen evolution in low pH-treated membranes became similar to that in NaCl-washed PS 11 preparations and the changes were largely reversed by rebinding of the two proteins. These results strongly suggest that low pH-treatment and NaCl-wash involve similar mechanisms of Ca^<2+>-dependent reactivation.