2021/07/12 更新

写真a

トミオカ ケンジ
富岡 憲治
TOMIOKA Kenji
所属
自然科学学域 教授
職名
教授
外部リンク

学位

  • 理学博士 ( 北海道大学 )

研究キーワード

  • 時間生物学

  • 神経行動学

  • Chronobiology

  • Neuroethology

研究分野

  • ライフサイエンス / 動物生理化学、生理学、行動学

学歴

  • 岡山大学   Graduate School, Division of Natural Science  

    - 1980年

      詳細を見る

  • 岡山大学    

    - 1980年

      詳細を見る

    国名: 日本国

    researchmap

  • 岡山大学   理学部   生物学科

    - 1978年

      詳細を見る

    国名: 日本国

    researchmap

  • 岡山大学   Faculty of Science  

    - 1978年

      詳細を見る

経歴

  • - 岡山大学自然科学研究科 教授

    2005年 - 現在

      詳細を見る

  • - Professor,Graduate School of Natural Science and Technology, Okayama University,Biological Sciences,Graduate School of Natural Science and Technology,Okayama University

    2005年

      詳細を見る

  • Professor,Okayama University, Faculty of Science, Department of Biology,Dept. of Biology,Faculty of Science,Okayama University

    2003年 - 2005年

      詳細を見る

  • 岡山大学理学部生物学科 教授   Faculty of Science, Department of Biology

    2003年 - 2005年

      詳細を見る

  • Professor

    1995年 - 2003年

      詳細を見る

  • 山口大学理学部 教授   Faculty of Science

    1995年 - 2003年

      詳細を見る

  • 山口大学理学部 助教授   Faculty of Science

    1992年 - 1995年

      詳細を見る

  • Associate Professor

    1992年 - 1995年

      詳細を見る

  • Research Associate,University of North Carolina at Chapel Hill

    1986年 - 1987年

      詳細を見る

  • ノースキャロライナ大学チャペルヒル校 博士研究員

    1986年 - 1987年

      詳細を見る

  • Research Associate

    1980年 - 1992年

      詳細を見る

  • 山口大学理学部 助手   Faculty of Science

    1980年 - 1992年

      詳細を見る

▼全件表示

所属学協会

  • 国際神経行動学会(International Society for Neuroethology)

    1995年 - 2005年

      詳細を見る

  • 生物リズム学会(Society for Research on Biological Rhythms)

    1991年 - 現在

      詳細を見る

  • 日本比較生理生化学会

    1980年 - 現在

      詳細を見る

  • 日本動物学会

    1978年 - 現在

      詳細を見る

  • 日本時間生物学会

      詳細を見る

  • ニューロエソロジー談話会

      詳細を見る

▼全件表示

委員歴

  • Physiological Entomology   Editorial Board (Associate Editor)  

    2018年 - 現在   

      詳細を見る

  • Zoological Letters   Editorial Board  

    2015年 - 現在   

      詳細を見る

  • Zoological Science   Advisory Board  

    2003年 - 現在   

      詳細を見る

  • Journal of Insect Physiology   Editorial Board  

    1992年 - 現在   

      詳細を見る

  • ニューロエソロジー談話会   世話人  

      詳細を見る

    団体区分:学協会

    ニューロエソロジー談話会

    researchmap

  • 日本比較生理生化学会   編集幹事  

      詳細を見る

    団体区分:学協会

    日本比較生理生化学会

    researchmap

  • 日本動物学会   評議員,代議員  

      詳細を見る

    団体区分:学協会

    日本動物学会

    researchmap

▼全件表示

 

論文

  • Photoperiod and temperature separately regulate nymphal development through JH and insulin/TOR signaling pathways in an insect 査読

    Taiki Miki, Tsugumichi Shinohara, Silvia Chafino, Sumihare Noji, Kenji Tomioka

    Proceedings of the National Academy of Sciences of the United States of America   117 ( 10 )   5525 - 5531   2020年3月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Proceedings of the National Academy of Sciences  

    DOI: 10.1073/pnas.1922747117

    researchmap

  • The role of clockwork orange in the circadian clock of the cricket Gryllus bimaculatus. 査読

    Tomiyama, Y, Shinohara, T, Matsuka, M, Bando, T, Mito, T, Tomioka, K

    Zoological Letters   in press   2020年

     詳細を見る

    担当区分:責任著者   記述言語:英語  

    researchmap

  • The compound eye possesses a self-sustaining circadian oscillator in the cricket Gryllus bimaculatus. 査読

    Ohguro, C, Moriyama, Y, Tomioka, K

    Zoological Sciene   in press   2020年

     詳細を見る

    担当区分:責任著者   記述言語:英語  

    researchmap

  • Functional analysis of Pdp1 and vrille in the circadian system of a cricket 査読

    Narasaki-Funo, Y, Tomiyama, Y, Nose, M, Bando, T, Tomioka, K

    Journal of Insect Physiology   in press   2020年

     詳細を見る

    担当区分:責任著者   記述言語:英語  

    researchmap

  • The circadian system in insects: Cellular, molecular, and functional organization. 査読

    Tomioka K, Matsumoto A

    Adv. Insect Physiol.   56   73 - 115   2019年

     詳細を見る

  • cryptochrome genes mediate photoperiodic responses in the cricket Modicogryllus siamensis 査読

    Haruki Ueda, Saori Tamaki, Taiki Miki, Outa Uryu, Yuichi Kamae, Motoki Nose, Tsugumichi Shinohara, Kenji Tomioka

    Physiological Entomology   43   285 - 294   2018年12月

     詳細を見る

    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/phen.12258

    researchmap

  • A novel photic entrainment mechanism for the circadian clock in an insect: involvement of c-fos and cryptochromes 査読

    Yuki Kutaragi, Atsushi Tokuoka, Yasuaki Tomiyama, Motoki Nose, Takayuki Watanabe, Tetsuya Bando, Yoshiyuki Moriyama, Kenji Tomioka

    Zoological Letters   4 ( 1 )   2018年12月

     詳細を見る

    掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media {LLC}  

    DOI: 10.1186/s40851-018-0109-8

    researchmap

  • timeless2 plays an important role in reproduction and circadian rhythms in the cricket Gryllus bimaculatus 査読

    Motoki Nose, Atsushi Tokuoka, Tetsuya Bando, Kenji Tomioka

    Journal of Insect Physiology   105   9 - 17   2018年2月

     詳細を見る

    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier Ltd  

    The timeless2 (tim2) gene is an insect orthologue of the mammalian clock gene Timeless (mTim). Although its functional role has been extensively studied in mammals, little is known regarding its role in insects. In the present study, we obtained tim2 cDNA (Gb'tim2) from the cricket Gryllus bimaculatus and characterized its functional role in embryonic development, egg production, and circadian rhythms. Gb'tim2 gave rise to a 1432 amino acid protein, and showed approximately 65% homology to that of Drosophila melanogaster. When treated with parental Gb'tim2RNAi, less than 2% of the treated eggs hatched. On the other hand, control eggs treated with DsRed2RNAi demonstrated a hatching rate of 70%. In most of the Gb'tim2RNAi treated embryos, development was arrested in early stages. Egg production in ovaries of adult virgin females treated with Gb'tim2RNAi was significantly reduced. In addition, while Gb'tim2RNAi crickets exhibited clear locomotor rhythm synchronized with light cycles, their light-on peak was weaker than that of control crickets. Under constant darkness, the activity rhythm of Gb'tim2RNAi crickets was often split into two components running with different periods. Molecular analysis revealed that Gb'tim2RNAi treatment downregulated mRNA levels of Gb'per and Gb'Clk, and enhanced Gb'cyc expression rhythm
    no distinct effect was found on Gb'tim expression levels. The change in Gb'per, Gb'Clk and Gb'cyc levels may underlie the altered behavioral rhythms in Gb'tim2RNAi crickets. Both Gb'ClkRNAi and Gb'cycRNAi downregulated Gb'tim2 expression, which suggested that transcription of Gb'tim2 is mediated by Gb'CLK and Gb'CYC through E-box. These results suggested that Gb'tim2 may be involved in both reproduction and circadian rhythm regulation in crickets.

    DOI: 10.1016/j.jinsphys.2017.12.007

    Scopus

    researchmap

  • A Tug-of-War between Cryptochrome and the Visual System Allows the Adaptation of Evening Activity to Long Photoperiods in Drosophila melanogaster 査読

    Christa Kistenpfennig, Mayumi Nakayama, Ruri Nihara, Kenji Tomioka, Charlotte Helfrich-Förster, Taishi Yoshii

    Journal of Biological Rhythms   33 ( 1 )   24 - 34   2018年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE Publications Inc.  

    In many animals, the circadian clock plays a role in adapting to the coming season by measuring day length. The mechanism for measuring day length and its neuronal circuits remains elusive, however. Under laboratory conditions, the fruit fly, Drosophila melanogaster, displays 2 activity peaks: one in the morning and one in the evening. These peaks appear to be regulated by 2 separate circadian oscillators (the morning and evening oscillators) that reside in different subsets of pacemaker clock neurons in the brain. The morning and evening activity peaks can flexibly change their phases to adapt to different photoperiods by tracking dawn and dusk, respectively. In this study, we found that cryptochrome (CRY) in the evening oscillators (the fifth small ventral lateral neuron [5th s-LNv] and the dorsal lateral neurons [LNds]) limits the ability of the evening peak to track dusk during long days. In contrast, light signaling from the external photoreceptors (compound eyes, ocelli, and Hofbauer–Buchner eyelets) increases the ability of the evening peak to track dusk. At the molecular level, CRY signaling dampens the amplitude of PAR-domain protein 1 (PDP1) oscillations in most clock neurons during long days, whereas signaling from the visual system increases these amplitudes. Thus, our results suggest that light inputs from the two major circadian photoreceptors, CRY and the visual system, have opposite effects on day length adaptation. Their tug-of-war appears to determine the precise phase adjustment of evening activity.

    DOI: 10.1177/0748730417738612

    Scopus

    researchmap

  • cryptochrome genes form an oscillatory loop independent of the per/tim loop in the circadian clockwork of the cricket Gryllus bimaculatus 査読

    Atsushi Tokuoka, Taichi Q. Itoh, Shinryo Hori, Outa Uryu, Yoshiki Danbara, Motoki Nose, Tetsuya Bando, Teiichi Tanimura, Kenji Tomioka

    Zoological Letters   3 ( 1 )   375 - 381   2017年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media {LLC}  

    Background: Animals exhibit circadian rhythms with a period of approximately 24 h in various physiological functions, including locomotor activity. This rhythm is controlled by an endogenous oscillatory mechanism, or circadian clock, which consists of cyclically expressed clock genes and their product proteins. cryptochrome (cry) genes are thought to be involved in the clock mechanism, and their functions have been examined extensively in holometabolous insects, but in hemimetabolous insects their role is less well understood.Results: In the present study, the role of cry genes was investigated using RNAi technology in a hemimetabolous insect, the cricket Gryllus bimaculatus. Using a molecular cloning approach, we obtained cDNAs for two cry genes: Drosophila-type cry1 (Gb ' cry1) and mammalian-type cry2 (Gb'cry2). Gb ' cry2 has six splicing variants, most of which showed rhythmic mRNA expression. Gb ' cry1(RNAi) treatment had only a limited effect at the behavioral and molecular levels, while Gb ' cry2(RNAi) had a significant effect on behavioral rhythms and molecular oscillatory machinery, alone or in combination with Gb'cry1RNAi. In Gb ' cry1/ Gb ' cry2 double-RNAi crickets, most clock genes showed arrhythmic expression, except for timeless, which retained clear rhythmic expression. Molecular analysis revealed that some combination of Gb ' cry1 and Gb ' cry2 variants suppressed CLK/CYC transcriptional activity in cultured cells.Conclusion: Based on these results, we propose a new model of the cricket's circadian clock, including a molecular oscillatory loop for Gb'cry2, which can operate independent of the Gb ' per/Gb ' tim loop.

    DOI: 10.1186/s40851-017-0066-7

    Web of Science

    researchmap

  • The effect of Wolbachia on diapause, fecundity, and clock gene expression in Trichogramma brassicae (Hymenoptera: Trichogrammatidae) 査読

    Somayeh Rahimi-Kaldeh, Ahmad Ashouri, Alireza Bandani, Kenji Tomioka

    DEVELOPMENT GENES AND EVOLUTION   227 ( 6 )   401 - 410   2017年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    The short day lengths of late summer in moderate regions are used to induce diapause in various insects. Many studies have shown the maternal effect of photoperiod on diapause induction of Trichogramma wasps, but there is no study to show the relationship between photoperiodic regimes and clock genes in these useful biological control agents. Here, we investigated the role of photoperiods on diapause, fecundity, and clock gene expression (clk, cyc, cry2, per, and timeout) in asexual and sexual Trichogramma brassicae as a model insect to find any differences between two strains. Asexual strain was infected by Wolbachia, an endosymbiont bacterium. The diapause percentage was significantly higher under short days (8 h in sexual and 12 h in the asexual T. brassicae), although the diapause percentage of the sexual strain was significantly higher than the asexual one in all the photoperiods. The ANOVA revealed no significant changes between different photoperiods in the clock gene expression in the sexual strain but significant photoperiodic changes in clk, cyc, and timeout in the asexual strain. Our results showed that the mRNA levels of clock genes of asexual T. brassicae were significantly lower than those of sexual strain. The fecundity was significantly higher in the asexual strain. These results suggest that Wolbachia infection makes disturbance on the clock gene expression which consequently reduces the percentage of diapause but increases the fecundity in asexual T. brassicae.

    DOI: 10.1007/s00427-017-0597-0

    Web of Science

    researchmap

  • Molecular approach to the circadian clock mechanism in the cricket 査読

    Kenji Tomioka, Outa Uryu, Yuichi Kamae, Yoshiyuki Moriyama, ASM Saifullah, Taishi Yoshii

    The Cricket as a Model Organism: Development, Regeneration, and Behavior   77 - 89   2017年1月

     詳細を見る

    記述言語:英語   掲載種別:論文集(書籍)内論文   出版者・発行元:Springer Japan  

    Circadian rhythms are physiological and behavioral changes that follow a roughly 24-h period, responding primarily to daily cycles in an organism’s environment. Crickets have provided a good model to study the neural mechanisms controlling the circadian rhythm, because they have a large central nervous system. Neurobiological studies revealed that the circadian clock is located in the optic lobe and the photoreceptors necessary for light entrainment are in the compound eye. Recent progress in molecular technology enabled us to use crickets for dissection of the circadian system at a molecular level. The oscillatory mechanism of the circadian clock has been studied in Drosophila and a few higher order insect species, but the results from those insects are often inconsistent. We employed a reverse genetic approach to the cricket clock. We first obtained clock genes, period (per), timeless (tim), and Clock (Clk) with molecular cloning and then analyzed their functions with RNAi technology. The obtained results could be only partially explained by the Drosophila model. The central oscillatory mechanism of the cricket clock will be discussed together with the peripheral oscillators and the involvement of pigment-dispersing factor as a neurotransmitter in regulating the locomotor rhythm.

    DOI: 10.1007/978-4-431-56478-2_6

    Scopus

    researchmap

  • Enhancer of zeste plays an important role in photoperiodic modulation of locomotor rhythm in the cricket, Gryllus bimaculatus. 査読 国際誌

    Yoshimasa Hamada, Atsushi Tokuoka, Tetsuya Bando, Hideyo Ohuchi, Kenji Tomioka

    Zoological letters   2 ( 5 )   5 - 5   2016年

     詳細を見る

    記述言語:英語  

    INTRODUCTION: Insects show daily behavioral rhythms controlled by an endogenous oscillator, the circadian clock. The rhythm synchronizes to daily light-dark cycles (LD) and changes waveform in association with seasonal change in photoperiod. RESULTS: To explore the molecular basis of the photoperiod-dependent changes in circadian locomotor rhythm, we investigated the role of a chromatin modifier, Enhancer of zeste (Gb'E(z)), in the cricket, Gryllus bimaculatus. Under a 12 h:12 h LD (LD 12:12), Gb'E(z) was constitutively expressed in the optic lobe, the site of the biological clock; active phase (α) and rest phase (ρ) were approximately 12 h in duration, and α/ρ ratio was approximately 1.0. When transferred to LD 20:4, the α/ρ ratio decreased significantly, and the Gb'E(z) expression level was significantly reduced at 6 h and 10 h after light-on, as was reflected in the reduced level of trimethylation of histone H3 lysine 27. This change was associated with change in clock gene expression profiles. The photoperiod-dependent changes in α/ρ ratio and clock gene expression profiles were prevented by knocking down Gb'E(z) by RNAi. CONCLUSIONS: These results suggest that histone modification by Gb'E(z) is involved in photoperiodic modulation of the G. bimaculatus circadian rhythm.

    DOI: 10.1186/s40851-016-0042-7

    PubMed

    researchmap

  • Interaction between sexes and between different circadian phenotypes affects lifespan in the fruit fly Drosophila melanogaster 査読

    Kawaguchi, T., Umezaki, Y., Ito, C., Tomioka, K.

    Physiological Entomology   41 ( 1 )   48 - 58   2016年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Circadian clocks regulate the daily temporal structure of physiological and behavioural functions. In the fruit fly Drosophila melanogaster Meigen, disruption of daily rhythms is suggested to reduce the fly's lifespan. In the present study, because pairs of mixed-sex flies are known to show an activity pattern different from that of individual flies, this hypothesis is tested by measuring the lifespan of flies housed same-sexually or mixed-sexually under an LD 12 : 12 h photocycle at a constant temperature of 25 degrees C. The effect of housing wild-type (Canton-S) flies with period (per) circadian clock mutant flies is also examined because the mutant flies have different daily activity patterns. When males and females of wild-type flies are housed together, their lifespan is substantially lengthened (males) or shortened (females) compared with same-sex housed flies. The shortening of the lifespan in females is significantly enhanced when mated with per mutant males. The shortening effects are significantly reduced when the mixed-sex interaction is limited for the first 5 days after emergence. A slight elongation in lifespan, rather than a reduction, occurs when wild-type females are housed same-sexually with per(0) or per(L) mutant flies. In male flies, the elongation of lifespan occurs not only when wild-type males are housed with wild-type, per(0) or per(L) females, but also when housed with per(0) or per(S) mutant males. Mixed-sex couples always show altered daily locomotor rhythms with an enhanced night-time activity, whereas same-sex couples show daily behavioural profiles slightly altered but essentially similar to a sum of the respective two flies. No significant correlation is found between the lifespan and reproductive capacity. These results suggest that the alteration of daily activity rhythms and sexual interaction may have significant impact on the fly's lifespan.

    DOI: 10.1111/phen.12124

    Web of Science

    Scopus

    researchmap

  • Heterogeneity of the peripheral circadian systems in Drosophila melanogaster: A review 査読

    Ito, C., Tomioka, K.

    Frontiers in Physiology   7 ( JAN )   2016年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:FRONTIERS MEDIA SA  

    Circadian rhythms in organisms are involved in many aspects of metabolism, physiology, and behavior. In many animals, these rhythms are produced by the circadian system consisting of a central clock located in the brain and peripheral clocks in various peripheral tissues. The oscillatory machinery and entrainment mechanism of peripheral clocks vary between different tissues and organs. The relationship between the central and peripheral clocks is also tissue-dependent. Here we review the heterogeneous nature of peripheral circadian clocks in the fruit fly Drosophila melanogaster and their dependence on the central clock, and discuss their significance in the temporal organization of physiology in peripheral tissues/organs.

    DOI: 10.3389/fphys.2016.00008

    Web of Science

    Scopus

    researchmap

  • TGF-β signaling in insects regulates metamorphosis via juvenile hormone biosynthesis 査読

    Yoshiyasu Ishimaru, Sayuri Tomonari, Yuji Matsuoka, Takahito Watanabe, Katsuyuki Miyawaki, Tetsuya Bando, Kenji Tomioka, Hideyo Ohuchi, Sumihare Noji, Taro Mito

    Proceedings of the National Academy of Sciences of the United States of America   113 ( 20 )   5634 - 5639   2016年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1073/pnas.1600612113

    Web of Science

    Scopus

    researchmap

  • Transcriptional and nontranscriptional events are involved in photic entrainment of the circadian clock in the cricket Gryllus bimaculatus 査読

    Kutaragi, Y., Miki, T., Bando, T., Tomioka, K.

    Physiological Entomology   41 ( 4 )   358 - 368   2016年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Most insects show daily activity rhythms that are controlled by endogenous circadian clocks. A basic property of the clock is entrainment to daily environmental cycles to run with an exact 24-h period. The entrainment is achieved mainly through resetting by light. The present study analyses the light resetting mechanism of the clock in first-instar nymphal and adult crickets Gryllus bimaculatus (De Geer). A 3-h light pulse given at early subjective night and late subjective night causes a phase delay and an advance, respectively, of adult locomotor rhythms. The magnitude of the shift caused by the light pulse at circadian time 12 h in constant darkness is significantly smaller than that caused by a 3-h extension of light phase. Measurement of mRNA levels during the light-induced phase shifts yielded basically similar results for nymphs and adults. When a 3-h light pulse is given at early night by light phase extension, an increase of Pdp1 occurs, which is followed by upregulation of the genes Clk and subsequently by per and tim. These changes are eliminated by RNA interference of opsin-long wavelength, which is expressed in the compound eye and encodes a green-sensitive opsin, the major photoreceptor for photic entrainment of the clock. No clear changes in mRNA levels are observed for light pulses given at late subjective night or at early subjective night after 24 h of constant darkness. These results suggest that the photic entrainment mechanism of the clock may be shared by nymphal and adult crickets, including transcriptional and nontranscriptional events.

    DOI: 10.1111/phen.12162

    Web of Science

    Scopus

    researchmap

  • Dedicated Issue - Biochronometry in Insects 査読

    Numata, H., Tomioka, K.

    Physiological Entomology   41 ( 4 )   2016年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/phen.12169

    Scopus

    researchmap

  • Circadian molecular clockworks in non-model insects 査読

    Tomioka, K., Matsumoto, A.

    Current Opinion in Insect Science   7   58 - 64   2015年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.cois.2014.12.006

    Scopus

    researchmap

  • Cryptochrome-dependent and -independent circadian entrainment circuits in Drosophila 査読

    Yoshii, T., Hermann-Luibl, C., Kistenpfennig, C., Schmid, B., Tomioka, K., Helfrich-Förster, C.

    Journal of Neuroscience   35 ( 15 )   6131 - 6141   2015年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC NEUROSCIENCE  

    Entrainment to environmental light/dark (LD) cycles is a central function of circadian clocks. In Drosophila, entrainment is achieved by Cryptochrome (CRY) and input from the visual system. During activation by brief light pulses, CRY triggers the degradation of TIMELESS and subsequent shift in circadian phase. This is less important for LD entrainment, leading to questions regarding light input circuits and mechanisms from the visual system. Recent studies show that different subsets of brain pacemaker clock neurons, the morning (M) and evening (E) oscillators, have distinct functions in light entrainment. However, the role of CRY in M and E oscillators for entrainment to LD cycles is unknown. Here, we address this question by selectively expressing CRY in different subsets of clock neurons in a cry-null (cry(0)) mutant background. We were able to rescue the light entrainment deficits of cry0 mutants by expressing CRY in Eoscillators but not in any other clock neurons. Par domain protein 1 molecular oscillations in the E, but not M, cells of cry0 mutants still responded to the LD phase delay. This residual light response was stemming from the visual system because it disappeared when all external photoreceptors were ablated genetically. We concluded that the E oscillators are the targets of light input via CRY and the visual system and are required for normal light entrainment.

    DOI: 10.1523/JNEUROSCI.0070-15.2015

    Web of Science

    Scopus

    researchmap

  • Leg regeneration is epigenetically regulated by histone H3K27 methylation in the cricket Gryllus bimaculatus 査読

    Hamada, Y., Bando, T., Nakamura, T., Ishimaru, Y., Mito, T., Noji, S., Tomioka, K., Ohuchi, H.

    Development (Cambridge)   142 ( 17 )   2916 - +   2015年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:COMPANY OF BIOLOGISTS LTD  

    Hemimetabolous insects such as the cricket Gryllus bimaculatus regenerate lost tissue parts using blastemal cells, a population of dedifferentiated proliferating cells. The expression of several factors that control epigenetic modification is upregulated in the blastema compared with differentiated tissue, suggesting that epigenetic changes in gene expression might control the differentiation status of blastema cells during regeneration. To clarify the molecular basis of epigenetic regulation during regeneration, we focused on the function of the Gryllus Enhancer of zeste [Gb'E(z)] and Ubiquitously transcribed tetratricopeptide repeat gene on the X chromosome (Gb'Utx) homologues, which regulate methylation and demethylation of histone H3 lysine 27 (H3K27), respectively. Methylated histone H3K27 in the regenerating leg was diminished by Gb'E(z)(RNAi) and was increased by Gb'Utx(RNAi). Regenerated Gb'E(z)(RNAi) cricket legs exhibited extra leg segment formation between the tibia and tarsus, and regenerated Gb'Utx(RNAi) cricket legs showed leg joint formation defects in the tarsus. In the Gb'E(z)(RNAi) regenerating leg, the Gb'dac expression domain expanded in the tarsus. By contrast, in the Gb'Utx(RNAi) regenerating leg, Gb'Egfr expression in the middle of the tarsus was diminished. These results suggest that regulation of the histone H3K27 methylation state is involved in the repatterning process during leg regeneration among cricket species via the epigenetic regulation of leg patterning gene expression.

    DOI: 10.1242/dev.122598

    Web of Science

    Scopus

    researchmap

  • Editorial overview: Development and regulation: Chronobiology in insects 査読

    Tomioka, K., Pyza, E.

    Current Opinion in Insect Science   7   2015年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.cois.2015.02.001

    Scopus

    researchmap

  • Green-sensitive opsin is the photoreceptor for photic entrainment of an insect circadian clock. 査読

    Komada S, Kamae Y, Koyanagi M, Tatewaki K, Hassaneen E, Saifullah ASM, Yoshii T, Terakita A, Tomioka K

    Zoological Letters   1   11   2015年

  • Chronobiology of crickets: a review 査読

    Tomioka, K.

    Zoological science   31 ( 10 )   2014年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.2108/zs140024

    Scopus

    researchmap

  • The nuclear receptor genes HR3 and E75 are required for the circadian rhythm in a primitive insect 査読

    Kamae, Y., Uryu, O., Miki, T., Tomioka, K.

    PLoS ONE   9 ( 12 )   e114899   2014年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Insect circadian rhythms are generated by a circadian clock consisting of transcriptional/translational feedback loops, in which CYCLE and CLOCK are the key elements in activating the transcription of various clock genes such as timeless (tim) and period (per). Although the transcriptional regulation of Clock (Clk) has been profoundly studied, little is known about the regulation of cycle (cyc). Here, we identify the orphan nuclear receptor genes HR3 and E75, which are orthologs of mammalian clock genes, Rora and Rev-erba, respectively, as factors involved in the rhythmic expression of the cyc gene in a primitive insect, the firebrat Thermobia domestica. Our results show that HR3 and E75 are rhythmically expressed, and their normal, rhythmic expression is required for the persistence of locomotor rhythms. Their RNAi considerably altered the rhythmic transcription of not only cyc but also tim. Surprisingly, the RNAi of HR3 revealed the rhythmic expression of Clk, suggesting that this ancestral insect species possesses the mechanisms for rhythmic expression of both cyc and Clk genes. When either HR3 or E75 was knocked down, tim, cyc, and Clk or tim and cyc, respectively, oscillated in phase, suggesting that the two genes play an important role in the regulation of the phase relationship among the clock genes. Interestingly, HR3 and E75 were also found to be involved in the regulation of ecdysis, suggesting that they interconnect the circadian clock and developmental processes.

    DOI: 10.1371/journal.pone.0114899

    Web of Science

    Scopus

    researchmap

  • Post-embryonic development of circadian oscillations within and outside the optic lobe in the cricket, gryllus bimaculatus 査読

    Uryu, O., Tomioka, K.

    Zoological Science   31 ( 4 )   237 - 243   2014年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

    The adult cricket Gryllus bimaculatus has a central clock in the optic lobe that regulates overt activity rhythms and secondary oscillators in the tissue outside the optic lobe. Here we investigated properties of the rhythmic expression of clock genes in the optic lobe and extra-optic lobe tissues in nymphs, and compared them with those of adults. In the optic lobe, mRNA of the clock genes period (per), timeless (tim), cycle (cyc) and Clock (Clk) were expressed in patterns similar to those in adult profiles, but at significantly lower levels. Among the extra-optic lobe tissues, the brain and TAG showed a rhythmic expression of per and tim, the mid-gut only in tim, and the anterior-stomach in none of the genes studied. The mRNA levels of clock genes were again significantly lower than those in adults. Unlike in adults, the brain and mid-gut lost their rhythms of clock gene expression in DD, and when the optic lobes were bilaterally removed. These results suggest that the rhythms outside the optic lobe are weak in nymphs, and may become robust after the imaginal molt.

    DOI: 10.2108/zs130230

    Web of Science

    Scopus

    researchmap

  • Sexual interactions influence the molecular oscillations in DN1 pacemaker neurons in Drosophila melanogaster 査読

    Hanafusa, S., Kawaguchi, T., Umezaki, Y., Tomioka, K., Yoshii, T.

    PLoS ONE   8 ( 12 )   e84495   2013年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Circadian rhythms can synchronize to environmental time cues, such as light, temperature, humidity, and food availability. Previous studies have suggested that these rhythms can also be entrained by social interactions. Here, we used Drosophila melanogaster as a model to study the influence of socio-sexual interactions on the circadian clock in behavior and pacemaker neurons. If two flies of opposite sex were paired and kept in a small space, the daily activity patterns of the two flies were clearly different from the sum of the activity of single male and female flies. Compared with single flies, paired flies were more active in the night and morning, were more active during females' active phase, and were less active during males' active phase. These behavioral phenotypes are related to courtship behavior, but not to the circadian clock. Nevertheless, in male-female pairs of flies with clocks at different speeds ( wild-type and per(S) flies), clock protein cycling in the DN1 pacemaker neurons in the male brain were slightly influenced by their partners. These results suggest that sexual interactions between male-female couples can serve as a weak zeitgeber for the DN1 pacemaker neurons, but the effect is not sufficient to alter rhythms of behavioral activity.

    DOI: 10.1371/journal.pone.0084495

    Web of Science

    Scopus

    researchmap

  • Long-term effect of systemic RNA interference on circadian clock genes in hemimetabolous insects 査読

    Uryu, O., Kamae, Y., Tomioka, K., Yoshii, T.

    Journal of Insect Physiology   59 ( 4 )   494 - 499   2013年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.jinsphys.2013.02.009

    Web of Science

    Scopus

    researchmap

  • Opsins are involved in nymphal photoperiodic responses in the cricket Modicogryllus siamensis 査読

    Tamaki, S., Takemoto, S., Uryu, O., Kamae, Y., Tomioka, K.

    Physiological Entomology   38 ( 2 )   163 - 172   2013年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    The cricket Modicogryllus siamensis Chopard shows photoperiod-dependent changes in the duration of nymphal development: nymphs become adult within 60days after hatching, undergoing seven moults under long-day conditions, whereas, under short-day conditions, nymphal development takes much longer (approximately 180days) with an increased number of moults. Because removal of the compound eyes alters this photoperiodic response, the eyes may be involved in light detection during the photoperiodic response. The role of opsins, expressed in the compound eye, is examined in the present study with reference to the photoperiodic response. Molecular cloning identifies cDNAs of three opsins, opsin-Ultra Violet (Ms'op-UV), opsin-Blue (Ms'op-B) and opsin-Long Wave (Ms'op-LW), and in situ hybridization reveals that the opsin genes are expressed in specific regions of the compound eye in a gene-specific manner. RNA interference (RNAi) technology using the opsin genes results in a partial disruption in the long-day responses; most of the treated crickets showed eight or more moults and up to 23.5% show a prolonged nymphal period that is typical of short-day responses. Under short-day conditions, op-UV RNAi crickets show earlier adult development, whereas no distinct alterations are observed in op-B and op-LW RNAi insects. The results suggest that the opsin genes may play differential roles in the photoperiodic response in the cricket and that the results can be at least partially explained in terms of the external coincidence model of photoperiodic time measurement.

    DOI: 10.1111/phen.12015

    Web of Science

    Scopus

    researchmap

  • The clock gene cycle plays an important role in the circadian clock of the cricket Gryllus bimaculatus 査読

    Uryu, O., Karpova, S.G., Tomioka, K.

    Journal of Insect Physiology   59 ( 7 )   697 - 704   2013年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    To dissect the molecular oscillatory mechanism of the circadian clock in the cricket Gryllus bimaculatus, we have cloned a cDNA of the clock gene cycle (Gb'cyc) and analyzed its structure and function. Gb'cyc contains four functional domains, i.e. bHLH, PAS-A, PAS-B and BCTR domains, and is expressed rhythmically in light dark cycles, peaking at mid night. The RNA interference (RNAi) of Clock (Gb'Clk) and period (Gb'per) reduced the Gb'cyc mRNA levels and abolished the rhythmic expression, suggesting that the rhythmic expression of Gb'cyc is regulated by a mechanism including Gb'Clk and Gb'per. These features are more similar to those of mammalian orthologue of cyc (Bmal1) than those of Drosophila cyc. A single treatment with double-stranded RNA (dsRNA) of Gb'cyc effectively knocked down the Gb'cyc mRNA level and abolished its rhythmic expression. The cyc RNAi failed to disrupt the locomotor rhythm, but lengthened its free-running period in constant darkness (DD). It is thus likely that Gb'cyc is involved in the circadian clock machinery of the cricket. The cyc RNAi crickets showed a rhythmic expression of Gb'per and timeless (Gb'tim) in the optic lobe in DD, explaining the persistence of the locomotor rhythm. Surprisingly, cyc RNAi revealed a rhythmic expression of Gb'Clk in DD which is otherwise rather constitutively expressed in the optic lobe. These facts suggest that the cricket might have a unique clock oscillatory mechanism in which both Gb'cyc and Gb'Clk are rhythmically controlled and that under abundant expression of Gb'cyc the rhythmic expression of Gb'Clk may be concealed. (C) 2013 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.jinsphys.2013.04.011

    Web of Science

    Scopus

    researchmap

  • Gb'clock is expressed in the optic lobe and is required for the circadian clock in the cricket Gryllus bimaculatus 査読

    Moriyama, Y., Kamae, Y., Uryu, O., Tomioka, K.

    Journal of Biological Rhythms   27 ( 6 )   467-477   2012年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1177/0748730412462207

    Scopus

    researchmap

  • Peripheral circadian rhythms and their regulatory mechanism in insects and some other arthropods: A review 査読

    Tomioka, K., Uryu, O., Kamae, Y., Umezaki, Y., Yoshii, T.

    Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology   182 ( 6 )   729 - 740   2012年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER HEIDELBERG  

    Many physiological functions of insects show a rhythmic change to adapt to daily environmental cycles. These rhythms are controlled by a multi-clock system. A principal clock located in the brain usually organizes the overall behavioral rhythms, so that it is called the "central clock". However, the rhythms observed in a variety of peripheral tissues are often driven by clocks that reside in those tissues. Such autonomous rhythms can be found in sensory organs, digestive and reproductive systems. Using Drosophila melanogaster as a model organism, researchers have revealed that the peripheral clocks are self-sustained oscillators with a molecular machinery slightly different from that of the central clock. However, individual clocks normally run in harmony with each other to keep a coordinated temporal structure within an animal. How can this be achieved? What is the molecular mechanism underlying the oscillation? Also how are the peripheral clocks entrained by light-dark cycles? There are still many questions remaining in this research field. In the last several years, molecular techniques have become available in non-model insects so that the molecular oscillatory mechanisms are comparatively investigated among different insects, which give us more hints to understand the essential regulatory mechanism of the multi-oscillatory system across insects and other arthropods. Here we review current knowledge on arthropod's peripheral clocks and discuss their physiological roles and molecular mechanisms.

    DOI: 10.1007/s00360-012-0651-1

    Web of Science

    Scopus

    researchmap

  • Pigment-dispersing factor is involved in age-dependent rhythm changes in Drosophila melanogaster 査読

    Umezaki, Y., Yoshii, T., Kawaguchi, T., Helfrich-Förster, C., Tomioka, K.

    Journal of Biological Rhythms   27 ( 6 )   423 - 432   2012年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1177/0748730412462206

    Web of Science

    Scopus

    researchmap

  • Timeless is an essential component of the circadian clock in a primitive insect, the firebrat thermobia domestica 査読

    Kamae, Y., Tomioka, K.

    Journal of Biological Rhythms   27 ( 2 )   126 - 134   2012年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE PUBLICATIONS INC  

    Recent studies show that the timeless (tim) gene is not an essential component of the circadian clock in some insects. In the present study, we have investigated whether the tim gene was originally involved in the insect clock or acquired as a clock component later during the course of evolution using an apterygote insect, Thermobia domestica. A cDNA of the clock gene tim (Td'tim) was cloned, and its structural analysis showed that Td'TIM includes 4 defined functional domains, that is, 2 regions for dimerization with PERIOD (PER-1, PER-2), nuclear localization signal (NLS), and cytoplasmic localization domain (CLD), like Drosophila TIM. Td'tim exhibited rhythmic expression in its mRNA levels with a peak during late day to early night in LD, and the rhythm persisted in DD. A single injection of double-stranded RNA (dsRNA) of Td'tim (dstim) into the abdomen of adult firebrats effectively knocked down mRNA levels of Td'tim and abolished its rhythmic expression. Most dsRNA-injected firebrats lost their circadian locomotor rhythm in DD up to 30 days after injection. DsRNA of cycle (cyc) and Clock genes also abolished the rhythmic expression of Td'tim mRNA by knocking down Td'tim mRNA to its basal level of intact firebrats, suggesting that the underlying molecular clock of firebrats resembles that of Drosophila. Interestingly, however, dstim also reduced cyc mRNA to its basal level of intact animals and eliminated its rhythmic expression, suggesting the involvement of Td'tim in the regulation of cyc expression. These results suggest that tim is an essential component of the circadian clock of the primitive insect T. domestica; thus, it might have been involved in the clock machinery from a very early stage of insect evolution, but its role might be different from that in Drosophila.

    DOI: 10.1177/0748730411435997

    Web of Science

    Scopus

    researchmap

  • Pigment-dispersing factor affects nocturnal activity rhythms, photic entrainment, and the free-running period of the circadian clock in the cricket gryllus bimaculatus 査読

    Hassaneen, E., El-Din Sallam, A., Abo-Ghalia, A., Moriyama, Y., Karpova, S.G., Abdelsalam, S., Matsushima, A., Shimohigashi, Y., Tomioka, K.

    Journal of Biological Rhythms   26 ( 1 )   3 - 13   2011年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE PUBLICATIONS INC  

    Pigment-dispersing factor (PDF) is a neuropeptide widely distributed in insect brains and plays important roles in the circadian system. In this study, we used RNA interference to study the role of the pigment-dispersing factor (pdf) gene in regulating circadian locomotor rhythms in the cricket, Gryllus bimaculatus. Injections of pdf double-stranded RNA (dspdf) effectively knocked down the pdf mRNA and PDF peptide levels. The treated crickets maintained the rhythm both under light-dark cycles (LD) and constant darkness (DD). However, they showed rhythms with reduced nocturnal activity with prominent peaks at lights-on and lights-off. Entrainability of dspdf-injected crickets was higher than control crickets as they required fewer cycles to resynchronize to the LD cycles shifted by 6 h. The free-running periods of the dspdf-injected crickets were shorter than those of control crickets in DD. These results suggest that PDF is not essential for the rhythm generation but involved in control of the nocturnality, photic entrainment, and fine tuning of the free-running period of the circadian clock.

    DOI: 10.1177/0748730410388746

    Web of Science

    Scopus

    researchmap

  • Blocking synaptic transmission with tetanus toxin light chain reveals modes of neurotransmission in the PDF-positive circadian clock neurons of Drosophila melanogaster 査読

    Umezaki, Y., Yasuyama, K., Nakagoshi, H., Tomioka, K.

    Journal of Insect Physiology   57 ( 9 )   1290 - 1299   2011年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Circadian locomotor rhythms of Drosophila melanogaster are controlled by a neuronal circuit composed of approximately 150 clock neurons that are roughly classified into seven groups. In the circuit, a group of neurons expressing pigment-dispersing factor (PDF) play an important role in organizing the pacemaking system. Recent studies imply that unknown chemical neurotransmitter(s) (UNT) other than PDF is also expressed in the PDF-positive neurons. To explore its role in the circadian pacemaker, we examined the circadian locomotor rhythms of pdf-Gal4/UAS-TNT transgenic flies in which chemical synaptic transmission in PDF-positive neurons was blocked by expressed tetanus toxin light chain (TNT). In constant darkness (DD), the flies showed a free-running rhythm, which was similar to that of wild-type flies but significantly different from pdf null mutants. Under constant light conditions (LL), however, they often showed complex rhythms with a short period and a long period component. The UNT is thus likely involved in the synaptic transmission in the clock network and its release caused by LL leads to arrhythmicity. Immunocytochemistry revealed that LL induced phase separation in TIMELESS (TIM) cycling among some of the PDF-positive and PDF-negative clock neurons in the transgenic flies. These results suggest that both PDF and UNT play important roles in the Drosophila circadian clock, and activation of PDF pathway alone by LL leads to the complex locomotor rhythm through desynchronized oscillation among some of the clock neurons. (C) 2011 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.jinsphys.2011.06.004

    Web of Science

    Scopus

    researchmap

  • Temperature entrainment of the circadian cuticle deposition rhythm in drosophila melanogaster 査読

    Ito, C., Goto, S.G., Tomioka, K., Numata, H.

    Journal of Biological Rhythms   26 ( 1 )   14 - 23   2011年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE PUBLICATIONS INC  

    The cuticle deposition rhythm, which is observed in the apodeme of the furca in the thorax, is controlled by a peripheral circadian clock in the epidermal cells and entrained to light-dark (LD) cycles via CRYPTOCHROME (CRY) in Drosophila melanogaster. In the present study, we examined the effects of temperature (TC) cycles and the combination of LD and TC cycles on entrainment of the cuticle deposition rhythm. The rhythm was entrained to TC cycles, whose period was 28 h. In T = 21 and 24 h, the rhythm was entrained to TC cycles in some individuals. CRY is not necessary for temperature entrainment of the cuticle deposition rhythm because the rhythm in cryb (lacking functional CRY) was entrained to TC cycles. Temperature entrainment of the rhythm was achieved even when the thoraxes or furcae were cultured in vitro, suggesting that the mechanism for temperature entrainment is independent of the central clock in the brain and the site of the thermoreception resides in the epidermal cells. When LD and TC cycles with different periods were applied, the rhythm was entrained to LD cycles with a slight influence of TC cycles. Thus, the LD cycle is a stronger zeitgeber than the TC cycle. The variance of the number of the cuticle layers decreased in the flies kept under LD and TC cycles with the same period in which the thermophase coincided with the photophase. Therefore, we conclude that LD and TC cycles synergistically entrain the rhythm. Synergistic effects of LD and TC cycles on entrainment were also observed even when the thoraxes were cultured in vitro, suggesting that the light and temperature information is integrated within the peripheral circadian system.

    DOI: 10.1177/0748730410391640

    Web of Science

    Scopus

    researchmap

  • Pigment-dispersing factor affects circadian molecular oscillations in the cricket Gryllus bimaculatus 査読

    Hassaneen, E., Sallam, A.E.-D., Abo-Ghalia, A., Moriyama, Y., Tomioka, K.

    Entomological Science   14 ( 3 )   278 - 282   2011年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Pigment-dispersing factor (PDF) is an important neurotransmitter in insect circadian systems. In the cricket Gryllus bimaculatus, it affects nocturnal activity, the free-running period and photic entrainment. In this study, to investigate whether these effects of PDF occur through a circadian molecular machinery, we measured mRNA levels of clock genes period (per) and timeless (tim) in crickets with pdf expression knocked-down by pdf RNAi. The pdf RNAi decreased per and tim mRNA levels during the night to reduce the amplitude of their oscillation. The phase of the rhythm advanced by about 4 h in terms of trough and/or peak phases. On the other hand, pdf mRNA levels were little affected by per and tim RNAi treatment. These results suggest that PDF affects the circadian rhythm at least in part through the circadian molecular oscillation while the circadian clock has little effect on the pdf expression.

    DOI: 10.1111/j.1479-8298.2011.00445.x

    Web of Science

    Scopus

    researchmap

  • A comparative view of insect circadian clock systems 査読

    Tomioka, K., Matsumoto, A.

    Cellular and Molecular Life Sciences   67 ( 9 )   1397 - 1406   2010年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER BASEL AG  

    Recent studies revealed that the neuronal network controlling overt rhythms shows striking similarity in various insect orders. The pigment-dispersing factor seems commonly involved in regulating locomotor activity. However, there are considerable variations in the molecular oscillatory mechanism, and input and output pathways among insects. In Drosophila, autoregulatory negative feedback loops that consist of clock genes, such as period and timeless are believed to create 24-h rhythmicity. Although similar clock genes have been found in some insects, the behavior of their product proteins shows considerable differences from that of Drosophila. In other insects, mammalian-type cryptochrome (cry2) seems to work as a transcriptional repressor in the feedback loop. For photic entrainment, Drosophila type cryptochrome (cry1) plays the major role in Drosophila while the compound eyes are the major photoreceptor in others. Further comparative study will be necessary to understand how this variety of clock mechanisms derived from an ancestral one.

    DOI: 10.1007/s00018-009-0232-y

    Web of Science

    Scopus

    researchmap

  • Circadian oscillations outside the optic lobe in the cricket Gryllus bimaculatus 査読

    Uryu, O., Tomioka, K.

    Journal of Insect Physiology   56 ( 9 )   1284 - 1290   2010年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Although circadian rhythms are found in many peripheral tissues in insects, the control mechanism is still to be elucidated. To investigate the central and peripheral relationships in the circadian organization, circadian rhythms outside the optic lobes were examined in the cricket Gryllus bimaculatus by measuring mRNA levels of period (per) and timeless (tim) genes in the brain, terminal abdominal ganglion (TAG), anterior stomach, mid-gut, testis, and Malpighian tubules. Except for Malpighian tubules and testis, the tissues showed a daily rhythmic expression in either both per and tim or tim alone in LD. Under constant darkness, however, the tested tissues exhibited rhythmic expression of per and tim mRNAs, suggesting that they include a circadian oscillator. The amplitude and the levels of the mRNA rhythms varied among those rhythmic tissues. Removal of the optic lobe, the central clock tissue, differentially affected the rhythms: the anterior stomach lost the rhythm of both per and tim; in the midgut and TAG, tim expression became arrhythmic but per maintained rhythmic expression; a persistent rhythm with a shifted phase was observed for both per and tim mRNA rhythms in the brain. These data suggest that rhythms outside the optic lobe receive control from the optic lobe to different degrees, and that the oscillatory mechanism may be different from that of Drosophila. (C) 2010 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.jinsphys.2010.04.010

    Web of Science

    Scopus

    researchmap

  • RNA interference of timeless gene does not disrupt circadian locomotor rhythms in the cricket Gryllus bimaculatus 査読

    Danbara, Y., Sakamoto, T., Uryu, O., Tomioka, K.

    Journal of Insect Physiology   56 ( 12 )   1738 - 1745   2010年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Molecular studies revealed that autoregulatory negative feedback loops consisting of so-called clock genes constitute the circadian clock in Drosophila However this hypothesis is not fully supported in other Insects and is thus to be examined In the cricket Gryllus bimaculatus we have previously shown that period (per) plays an essential role in the rhythm generation In the present study we cloned cDNA of the clock gene timeless (rim) and investigated its role in the cricket circadian oscillatory mechanism using RNA interference Molecular structure of the cricket tim has rather high similarity to those of other insect species Real-time RT-PCR analysis revealed that rim mRNA showed rhythmic expression in both LD and DD similar to that of per peaking during the (subjective) night When injected with rim double-stranded RNA (dstim) tim mRNA levels were significantly reduced and its circadian expression rhythm was eliminated After the dstim treatment however adult crickets showed a clear locomotor rhythm in DD with a free-running period significantly shorter than that of control crickets injected with Discosoma sp Red2 (DsRed2) dsRNA These results suggest that in the cricket rim plays some role in fine-tuning of the free-running period but may not be essential for oscillation of the circadian clock (C) 2010 Elsevier Ltd All rights reserved

    DOI: 10.1016/j.jinsphys.2010.07.002

    Web of Science

    Scopus

    researchmap

  • The clock gene cryptochrome of Bactrocera cucurbitae (Diptera: Tephritidae) in strains with different mating times 査読

    Fuchikawa, T., Sanada, S., Nishio, R., Matsumoto, A., Matsuyama, T., Yamagishi, M., Tomioka, K., Tanimura, T., Miyatake, T.

    Heredity   104 ( 4 )   387 - 392   2010年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/hdy.2009.167

    Scopus

    researchmap

  • Molecular cloning and functional analysis of the clock genes, Clock and cycle, in the firebrat Thermobia domestica 査読

    Kamae, Y., Tanaka, F., Tomioka, K.

    Journal of Insect Physiology   56 ( 9 )   1291 - 1299   2010年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Comparative molecular analysis reveals a wide variation of clock mechanisms among insects. In this study, the clock gene homologues of Clock (Td'Clk) and cycle (Td'cyc) were cloned from an apterygote insect, Thermobia domestica. Structural analysis showed that Td'CLK includes bHLH, PAS-A, PAS-B domains but lacks a polyglutamine repeat in the C terminal region that is implicated for transcriptional activity in Drosophila CLK. Td'CYC contains a BCTR domain in its C terminal in addition to the common domains found in Drosophila CYC, i.e. bHLH, PAS-A, PAS-B domains. Unlike in Drosophila, Td'Clk mRNA levels showed no significant daily fluctuation, while Td'cyc exhibited rhythmic expression. A single injection of double-stranded RNA (dsRNA) of Td'Clk or Td'cyc into the abdomen of adult firebrats effectively knocked down respective mRNA levels and abolished the rhythmic expression of Td'cyc. Most Td'Clk or Td'cyc dsRNA-injected firebrats lost their circadian locomotor rhythm in constant darkness up to 30 days after injection, whereas those injected with DsRed2 dsRNA as a negative control clearly maintained it. From these results, it is likely that Td'Clk and Td'cyc are involved in the circadian clock machinery in the firebrat. However, the structure and expression profile of Td'Clk and Td'cyc more closely resembles those of mammals than Drosophila. (C) 2010 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.jinsphys.2010.04.012

    Web of Science

    Scopus

    researchmap

  • Functional analysis of the circadian clock gene period by RNA interference in nymphal crickets Gryllus bimaculatus 査読

    Yoshiyuki Moriyama, Tomoaki Sakamoto, Akira Matsumoto, Sumihare Noji, Kenji Tomioka

    JOURNAL OF INSECT PHYSIOLOGY   55 ( 2 )   183 - 187   2009年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    The circadian clock gene period (Gryllus bimaculatus period, Gb'per) plays a core role in circadian rhythm generation in adults of the cricket Gryllus bimaculatus. We examined the role of Gb'per in nymphal crickets that show a diurnal rhythm rather than the nocturnal rhythm of the adults. As in the adult optic lobes, Gb'per mRNA levels in the head of the third instar nymphs showed daily cycling in light-dark cycles with a peak at mid night, and the rhythm persisted in constant darkness. Injection of Gb'per double-stranded RNA (dsRNA) into the abdomen of third instar nymphs knocked-down the mRNA levels to 25% of that in control animals. Most Gb'per dsRNA injected nymphs lost their circadian locomotor activity rhythm, while those injected with DsRed2 dsRNA as a negative control clearly maintained the rhythm. These results suggest that nymphs and adults share a common endogenous clock mechanism involving the clock gene Gb'per. (c) 2008 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.jinsphys.2008.11.005

    Web of Science

    researchmap

  • The clock gene period plays an essential role in photoperiodic control of nymphal development in the cricket modicogryllus siamensis 査読

    Sakamoto, T., Uryu, O., Tomioka, K.

    Journal of Biological Rhythms   24 ( 5 )   379 - 390   2009年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE PUBLICATIONS INC  

    Photoperiodic regulation of development is a common strategy for insects in the temperate zone to adapt to the seasonally changing environment. Although the circadian clock is generally thought to be involved, the underlying time measurement mechanism is still elusive. Here, we demonstrate that the circadian clock gene period (per) plays an essential role in the photoperiodic regulation of nymphal development in the cricket Modicogryllus siamensis. Nymphal development of this cricket depends on photoperiods, being accelerated by long days and slowed down by short days. We examined the role of per in the nymphal photoperiodic response as well as circadian rhythm generation using parental RNA interference (pRNAi). per mRNA levels in nymphal heads showed a rhythmic expression with the pattern dependent on photoperiods, and pRNAi significantly suppressed the per mRNA level with no significant rhythmicity in the early nymphal stage. Irrespective of photoperiods, nymphs treated with per pRNAi showed adult emergence patterns neither of intact nymphs nor of DsRed2 pRNAi nymphs kept under long days or under short days but similar to those kept under constant dark conditions. Most per pRNAi adults showed arrhythmic or aberrant circadian locomotor activity. These results suggest that the photoperiodic time measurement requires the normal circadian clock that is controlled by the per gene.

    DOI: 10.1177/0748730409341523

    Web of Science

    Scopus

    researchmap

  • RNA interference is a powerful tool for chronobiological study in the cricket 査読

    Tomioka, K., Sakamoto, T., Moriyama, Y.

    Sleep and Biological Rhythms   7 ( 3 )   144 - 151   2009年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Circadian rhythms are periodic phenomena recurring with a period of about 24 h that are observed in a variety of physiological functions. The rhythms are driven by an endogenous timing system involving the circadian clock. While recent molecular studies promoted understanding the oscillatory mechanism of the clock in model organisms such as Drosophila, its applicability to other insects still remains to be examined. RNA interference (RNAi) becomes a powerful method to dissect the clock and clock-related mechanisms in non-model insects. Crickets are particularly suitable for the application of RNAi, because a single injection of a clock gene dsRNA has long-lasting effects both on mRNA and behavioral rhythms. In this review, we introduce our current approach to the circadian clock as well as photoperiodic time-measurement mechanism in crickets, and describe the effectiveness of nymphal, adult, and parental RNAi in studies of biological timing mechanisms.

    DOI: 10.1111/j.1479-8425.2009.00407.x

    Web of Science

    Scopus

    researchmap

  • Functional analysis of the circadian clock gene period by RNA interference in nymphal crickets Gryllus bimaculatus 査読

    Moriyama, Y., Sakamoto, T., Matsumoto, A., Noji, S., Tomioka, K.

    Journal of Insect Physiology   55 ( 5 )   396 - 400   2009年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    The circadian clock gene period (Gryllus bimaculatus period, Gb'per) plays a core role in circadian rhythm generation in adults of the cricket Gryllus bimaculatus. We examined the role of Gb'per in nymphal crickets that show a diurnal rhythm rather than the nocturnal rhythm of the adults. As in the adult optic lobes, Gb'per mRNA levels in the head of the third instar nymphs showed daily cycling in light-dark cycles with a peak at mid night, and the rhythm persisted in constant darkness. Injection of Gb'per double-stranded RNA (dsRNA) into the abdomen of third instar nymphs knocked-down the mRNA levels to 25% of that in control animals. Most Gb'per dsRNA injected nymphs lost their circadian locomotor activity rhythm, while those injected with DsRed2 dsRNA as a negative control clearly maintained the rhythm. These results suggest that nymphs and adults share a common endogenous clock mechanism involving the clock gene Gb'per. (C) 2008 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.jinsphys.2008.11.005

    Web of Science

    Scopus

    researchmap

  • Functional analysis of the circadian clock gene period disrupts circadian rhythms in the cricket Gryllus bimaculatus. 査読

    Moriyama Y, Sakamoto T, Matsumoto A, Tomioka K

    J. Insect Physiol.   55   183 - 187   2008年

  • Peripheral circadian clock for the cuticle deposition rhythm in Drosophila melanogaster 査読

    Ito, C., Goto, S.G., Shiga, S., Tomioka, K., Numata, H.

    Proceedings of the National Academy of Sciences of the United States of America   105 ( 24 )   8446 - 8451   2008年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATL ACAD SCIENCES  

    Insect endocuticle thickens after adult emergence by daily alternating deposition of two chitin layers with different orientation. Although the cuticle deposition rhythm is known to be controlled by a circadian clock in many insects, the site of the driving clock, the photoreceptor for entrainment, and the oscillatory mechanism remain elusive. Here, we show that the cuticle deposition rhythm is regulated by a peripheral oscillator in the epidermis in Drosophila melanogaster. Free-running and entrainment experiments in vitro reveal that the oscillator for the cuticle deposition rhythm is independent of the central clock in the brain driving the locomotor rhythms. The cuticle deposition rhythm is absent in null and dominant-negative mutants of clock genes (i.e., period, timeless, cycle, and Clock), indicating that this oscillator is composed of the same clock genes as the central clock. Entrainment experiments with monochromatic light-dark cycles and cry(b) flies reveal that a blue light-absorbing photoreceptor, cryptochrome (CRY), acts as a photoreceptor pigment for the entrainment of the cuticle deposition rhythm. Unlike other peripheral rhythms in D. melanogaster, the cuticle deposition rhythm persisted in cry(b) and cry(OUT) mutant flies, indicating that CRY does not play a core role in the rhythm generation in the epidermal oscillator.

    DOI: 10.1073/pnas.0800145105

    Web of Science

    Scopus

    researchmap

  • Characterization of PDF-immunoreactive neurons in the optic lobe and cerebral lobe of the cricket, Gryllus bimaculatus 査読

    Abdelsalam, S., Uemura, H., Umezaki, Y., Saifullah, A.S.M., Shimohigashi, M., Tomioka, K.

    Journal of Insect Physiology   54 ( 7 )   1205 - 1212   2008年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Pigment-dispersing factor (PDF) is a neuropeptide playing important roles in insect circadian systems. In this study, we morphologically and physiologically characterized PDF-immunoreactive neurons in the optic lobe and the brain of the cricket Gryllus bimaculatus. PDF-immunoreactivity was detected in cells located in the proximal medulla (PDFMe cells) and those in the dorsal and ventral regions of the outer chiasma (PDFLa cells). The PDFMe cells had varicose processes spread over the frontal surface of the medulla and the PDFLa cells had varicose mesh-like innervations in almost whole lamina, suggesting their modulatory role in the optic lobe. Some of PDFMe cells had a hairpin-shaped axonal process running toward the lamina then turning back to project into the brain where they terminated at various protocerebral areas. The PDFMe cells had a low frequency spontaneous spike activity that was higher during the night and was often slightly increased by light pulses. Six pairs of PDF-immunoreactive neurons were also found in the frontal ganglion. Competitive ELISA with anti-PDF antibodies revealed daily cycling of PDF both in the optic lobe and cerebral lobe with an increase during the night that persisted in constant darkness. The physiological role of PDF is discussed based on these results. (C) 2008 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.jinsphys.2008.05.001

    Web of Science

    Scopus

    researchmap

  • PDF as a coupling mediator between the light-entrainable and temperature-entrainable clocks in Drosophila melanogaster 査読

    Tomioka, K., Miyasako, Y., Umezaki, Y.

    Acta Biologica Hungarica   59 ( SUPPL. )   149 - 155   2008年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AKADEMIAI KIADO  

    Drosophila shows bimodal circadian locomotor rhythms with peaks around light-on (morning peak) and before light-off (evening peak). The rhythm synchronizes to light and temperature cycles and the synchronization is achieved by two sets of clocks: one entrains to light cycles and the other to temperature cycles. The light-entrainable clock consists of the clock neurons located in the lateral protocerebrum (LNs) and the temperature-entrainable clock involves those located in the dorsal protocerebrum (DNs) and the cells located in the posterior lateral protocerebrum (LPNs). To understand the interaction between the light-entrainable and the temperature-entrainable clock neurons, locomotor rhythms of the mutant flies lacking PDF or PDF-positive clock neurons were examined. Under the light cycles, they showed altered phase of the evening peak. When exposed to temperature cycles of lower temperature levels, the onset of evening peak showed larger advance in contrast to those of wild-type flies. The termination of the peak also advanced while that of wild-type flies remained almost at the same phase as in the constant temperature. These results support our hypothesis that the PDF-positive light entrainable cells regulate the phase of the temperature entrainable cells to be synchronized to their own phase using PDF as a coupling mediator.

    DOI: 10.1556/ABiol.59.2008.Suppl.23

    Web of Science

    Scopus

    researchmap

  • Loss-of-function analyses of the fragile X-related and dopamine receptor genes by RNA interference in the cricket Gryllus bimaculatus 査読

    Hamada, A., Miyawaki, K., Honda-sumi, E., Tomioka, K., Mito, T., Ohuchi, H., Noji, S.

    Developmental Dynamics   238 ( 8 )   2025 - 2033   2008年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    In order to explore a possibility that the cricket Gryllus bimaculatus would be a useful model to unveil molecular mechanisms of human diseases, we performed loss-of-function analyses of Gryllus genes homologous to human genes that are responsible for human disorders, fragile X mental retardation 1 (fmr1) and Dopamine receptor (DopR). We cloned cDNAs of their Gryllus homologues, Gb'fmr1, Gb'DopRI, and Gb'DopRII, and analyzed their functions with use of nymphal RNA interference (RNAi). For Gb'fmr1, three major phenotypes were observed: (1) abnormal wing postures, (2) abnormal calling song, and (3) loss of the circadian locomotor rhythm, while for Gb'DopRI, defects of wing posture and morphology were found. These results indicate that the cricket has the potential to become a novel model system to explore human neuronal pathogenic mechanisms and to screen therapeutic drugs by RNAi. Developmental Dynamics 238. 2025-2033, 2009. (C) 2009 Wiley-Liss, Inc.

    DOI: 10.1002/dvdy.22029

    Web of Science

    Scopus

    researchmap

  • RNA interference of the clock gene period disrupts circadian rhythms in the cricket Gryllus bimaculatus 査読

    Moriyama, Y., Sakamoto, T., Karpova, S.G., Matsumoto, A., Noji, S., Tomioka, K.

    Journal of Biological Rhythms   23 ( 4 )   308 - 318   2008年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE PUBLICATIONS INC  

    Periodic expression of so-called clock genes is an essential part of the circadian clock. In Drosophila melanogaster the cyclic expression of per and tim through an autoregulatory feedback loop is believed to play a central role in circadian rhythm generation. However, it is still elusive whether this hypothesis is applicable to other insect species. Here it is shown that per gene plays a key role in the rhythm generation in the cricket Gryllus bimaculatus. Measurement of per mRNA levels in the optic lobe revealed the rhythmic expression of per in light cycles with a peak in the late day to early night, persisting in constant darkness. A single injection of per double-stranded RNA ( dsRNA) into the abdomen of the final instar nymphs effectively knocked down the mRNA levels as adult to about 50% of control animals. Most of the per dsRNA-injected crickets completely lost the circadian locomotor activity rhythm in constant darkness up to 50 days after the injection, whereas those injected with DsRed2 dsRNA as a negative control clearly maintained it. The electrical activity of optic lobe efferents also became arrhythmic in the per dsRNA-injected crickets. These results not only suggest that per plays an important role in the circadian rhythm generation also in the cricket but also show that RNA interference is a powerful tool to dissect the molecular machinery of the cricket circadian clock.

    DOI: 10.1177/0748730408320486

    Web of Science

    Scopus

    researchmap

  • Behavioral dissection of the Drosophila circadian multioscillator system that regulates locomotor rhythms 査読

    Umezaki, Y., Tomioka, K.

    Zoological Science   25 ( 11 )   1146 - 1155   2008年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

    The fruit fly, Drosophila melanogaster, shows a bimodal circadian activity rhythm with peaks around light-on and before light-off. This rhythm is driven by seven groups of so-called clock neurons in the brain. To dissect the multioscillatory nature of the Drosophila clock system, the process of reentrainment to a reversed light cycle was examined by using wild-type flies and cry(b) mutant flies that carry a strong loss-of-function mutation in cryptochrome (cry) gene. The wild-type flies showed that the morning peak dissociated into two components, while a substantial fraction of cry(b) flies exhibited dissociation of the evening peak into two components that shifted in different directions. When the temperature cycle was given in constant darkness in such a manner that the thermophase corresponded to the previous night phase, the morning peak also split into two components in wild-type flies. These results suggest that both morning and evening peaks are driven by two separate oscillators that have different entrainability to light and temperature cycles. Examination of the process of reentrainment to a reversed LD in mutant flies that lack some of the four known circadian photoreceptors (compound eyes, ocelli, CRYPTOCHROME [CRY], and Hofbauer-Buchner [H-B] eyelets) revealed that these four photoreceptors play different roles in photic entrainment of the four putative oscillators.

    DOI: 10.2108/zsj.25.1146

    Web of Science

    Scopus

    researchmap

  • Separate sets of cerebral clock neurons are responsible for light and temperature entrainment of Drosophila circadian locomotor rhythms 査読

    Miyasako, Y., Umezaki, Y., Tomioka, K.

    Journal of Biological Rhythms   22 ( 2 )   115 - 126   2007年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE PUBLICATIONS INC  

    The fruit fly Drosophila melanogaster shows a bimodal circadian locomotor rhythm with peaks at lights-on and before lights-off, which are regulated by multiple clocks in the brain. Even under light-dark cycles, the timing of the evening peak is highly dependent on temperature, starting earlier under lower ambient temperature but terminating almost at the same time. In the present study, using behavioral and immunohistochemical assays, the authors show that separate groups of clock neurons, either light-entrainable or temperature-entrainable, form a functional system driving the locomotor rhythm. When subjected to a light cycle combined with a temperature cycle advanced by 6 h relative to the light cycle, the dorsally located neurons (DNs) and lateral posterior neurons (LPNs) shifted their phase of TIMELESS expression, but the laterally located protocerebral neurons (LNs) basically maintained their original phase. Thus, the LNs seem to be preferentially light-entrainable and the DNs and LPNs to be primarily temperature-entrainable. In pdf(01) mutant flies that lack the neuropeptide PDF in the ventral groups of LNs, the onset of the evening peak was greatly advanced even under synchronized light and temperature cycles and was shifted even more than in wild-type flies in response to a 6-h phase shift of the temperature cycle, suggesting that ventral LNs have a strong impact on the phase of the other cells. It seems likely that the 2 sets of clock cells with different entrainability to light and temperature, and the coupling between them, enable Drosophila to keep a proper phase relationship of circadian activity with respect to the daily light and temperature cycles.

    DOI: 10.1177/0748730407299344

    Web of Science

    Scopus

    researchmap

  • Effects of unilateral compound-eye removal on the photoperiodic responses of nymphal development in the cricket Modicogryllus siamensis 査読

    Sakamoto, T., Tomioka, K.

    Zoological Science   24 ( 6 )   604 - 610   2007年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

    The cricket, Modicogryllus siamensis, shows clear photoperiodic responses at 25 degrees C in nymphal development. Under long-day conditions (LD16:8), nymphs became adults about 50 days after hatching, while under short-day conditions (LD8:16) the duration of nymphal stage extended to more than 130 days. Under constant dark conditions, two developmental patterns were observed: about 60% of crickets became adults slightly slower than under the long-day conditions, and the rest at later than 100 days after hatching, like those under the short-day conditions. When the compound eye was unilaterally removed on the 2nd day of hatching, an increase of molting and an extension of the nymphal period were observed under the long-day conditions, while under the short-day conditions, some crickets developed faster and others slower than intact crickets. These results suggest that this cricket receives photoperiodic information through the compound eye, that a pair of the compound eyes is required for a complete photoperiodic response, and that interaction between bilateral circadian clocks may be also involved in the response.

    DOI: 10.2108/zsj.24.604

    Web of Science

    Scopus

    researchmap

  • Induction of Drosophila behavioral and molecular circadian rhythms by temperature steps in constant light 査読

    Yoshii, T., Fujii, K., Tomioka, K.

    Journal of Biological Rhythms   22 ( 2 )   103 - 114   2007年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE PUBLICATIONS INC  

    In constant light, where Drosophila rhythms are normally disrupted, temperature cycles induce circadian rhythms at both the molecular and behavioral level. The authors investigated the process by which the thermoperiod induces the rhythms using temperature steps. A 10 degrees C temperature step-up induced a single locomotor activity peak ca 9 h after the temperature transition, whereas a 10 degrees C step-down induced a strong activity peak ca 24 h after the transition, and the peak recurred for several cycles, suggesting that the underlying clock is reset. Arrhythmic per(01), tim(01), dClk(lrk), and cyc(01) mutant flies failed to show the rhythm after the step-down, suggesting that per, tim, dClk, and cyc are necessary for the step-down-induced rhythm. After the step-up, per" flies exhibited an activity peak similar to that of wild-type flies, suggesting that the peak can be induced by the step-up in absence of PER. mRNA levels of-per, tim, dClk, vri, and Pdp1 epsilon were changed in response to the temperature steps, but the changes differed depending on the direction of temperature steps, suggesting that steps-up and steps-down have different roles in the initiation of the oscillation. Probably, alternating 12-h temperature steps-up and steps-down will induce opposite changes in mRNA levels of clock genes, eventually producing stable molecular oscillations. Although TIM shows responses to temperature consistent with the changes of its mRNA, this is not the case for PER, consistent with post-transcriptional regulation. Changes of the mRNA levels were significantly altered but still observed in per(01) flies but not observed in dClk(jrk) flies, except for per mRNA. This suggests that dCLK is involved in the temperature-induced changes in the levels of most clock gene mRNA but that per is regulated via a different mechanism.

    DOI: 10.1177/0748730406298176

    Web of Science

    Scopus

    researchmap

  • Functional analysis of circadian pacemaker neurons in Drosophila melanogaster 査読

    Rieger, D., Shafer, O.T., Tomioka, K., Helfrich-Förster, C.

    Journal of Neuroscience   26 ( 9 )   2531 - 2543   2006年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC NEUROSCIENCE  

    The molecular mechanisms of circadian rhythms are well known, but how multiple clocks within one organism generate a structured rhythmic output remains a mystery. Many animals show bimodal activity rhythms with morning ( M) and evening ( E) activity bouts. One long-standing model assumes that two mutually coupled oscillators underlie these bouts and show different sensitivities to light. Three groups of lateral neurons (LN) and three groups of dorsal neurons govern behavioral rhythmicity of Drosophila. Recent data suggest that two groups of the LN ( the ventral subset of the small LN cells and the dorsal subset of LN cells) are plausible candidates for the M and E oscillator, respectively. We provide evidence that these neuronal groups respond differently to light and can be completely desynchronized from one another by constant light, leading to two activity components that free-run with different periods. As expected, a long-period component started from the E activity bout. However, a short-period component originated not exclusively from the morning peak but more prominently from the evening peak. This reveals an interesting deviation from the original Pittendrigh and Daan ( 1976) model and suggests that a subgroup of the ventral subset of the small LN acts as "main" oscillator controlling M and E activity bouts in Drosophila.

    DOI: 10.1523/JNEUROSCI.1234-05.2006

    Web of Science

    Scopus

    researchmap

  • Entrainment of Drosophila circadian rhythms by temperature cycles 査読

    Tomioka, K., Yoshii, T.

    Sleep and Biological Rhythms   4 ( 3 )   240 - 247   2006年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The fruit fly, Drosophila melanogaster, has been a good organism for elucidating the molecular and cellular bases of circadian behavioral rhythms. The fly shows a bimodal locomotor rhythm with a period slightly different from 24-h. The rhythm synchronizes with the environmental cycle using light as a powerful zeitgeber. It is now believed that this rhythm is generated by two interlocked transcriptional feedback loops consisting of so-called clock genes. Light resets the clock loop through degradation of a clock protein, TIMELESS. Temperature is another powerful zeitgeber that can entrain the rhythm but the cellular and molecular basis for temperature entrainment is still under investigation. This review will try to give an overview of temperature entrainment, considering multiple clocks with different responsiveness to light and temperature. © 2006 The Authors
    Journal compilation © 2006 Japanese Society of Sleep Research.

    DOI: 10.1111/j.1479-8425.2006.00227.x

    Scopus

    researchmap

  • Postembryonic changes in circadian photo-responsiveness rhythms of optic lobe interneurons in the cricket Gryllus bimaculatus 査読

    Uemura, H., Tomioka, K.

    Journal of Biological Rhythms   21 ( 4 )   279 - 289   2006年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SAGE PUBLICATIONS INC  

    The photo-responsiveness of 2 groups of interneurons responding to light in the protocerebrum was investigated at 2 developmental stages, the last instar nymphs and adults, in the cricket Gryllus bimaculatus. The cricket is diurnally active during the nymphal stage but becomes nocturnal as an adult. In both adults and nymphs, light-induced responses of optic lobe light-responding interneurons that conduct light information from the optic medulla to the lobula and the cerebral lobe showed a circadian rhythm peaking during the subjective night. Amplitudes of the rhythms were not significantly different between adults and nymphs, but adults showed more stable day and night states than did nymphs. The medulla bilateral neurons that interconnect the bilateral medulla areas of the optic lobe also showed circadian rhythms in their light-induced responses in both adults and nymphs. The rhythm had a clear peak and a trough in adults, and its amplitude was significantly greater than that of nymphs. These results suggest that the 2 classes of interneurons are differentially controlled by the circadian clock. The difference might be related to their functional roles in the animal's circadian behavioral organization.

    DOI: 10.1177/0748730406288716

    Web of Science

    Scopus

    researchmap

  • History dependence of insect circadian rhythms 査読

    Tomioka, K, Sakamoto, T

    Formosan Entomologist   26, 87-97   2006年

     詳細を見る

  • Temperature cycles drive Drosophila circadian oscillation in constant light that otherwise induces behavioural arrhythmicity 査読

    Yoshii, T., Heshiki, Y., Ibuki-Ishibashi, T., Matsumoto, A., Tanimura, T., Tomioka, K.

    European Journal of Neuroscience   22 ( 5 )   1176 - 1184   2005年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING  

    The fruit fly, Drosophila melanogaster, shows a clear circadian locomotor rhythm in light cycles and constant darkness. Although the rhythm disappears in constant light, we found that temperature cycles drive the circadian rhythm both in locomotor activity and molecular abundance of PERIOD (PER) and TIMELESS (TIM). The thermoperiodically induced locomotor rhythm entailed an anticipatory activity at the late thermophase, which required several transient cycles to establish a steady-state entrainment, suggesting that the rhythm is endogenous and driven by a circadian clock. Western blot analysis revealed that PER and TIM increased during the cryophase, peaking at the middle to late cryophase. PER was also cyclically expressed under the temperature cycle in the known per-expressing neurons, i.e. so-called lateral (LNs) and dorsal neurons (DNs), and two pairs of cells (LPNs) that were located in the lateral posterior protocerebrum. It is thus suggested that the temperature cycle induces the cycling of PER and TIM either by blocking somewhere in the photic entrainment pathway during the cryophase or temporally activating their translation to sufficient protein levels to drive a circadian oscillation. In flies lacking pigment-dispersing factor (PDF) or PDF-expressing cells, the anticipatory activity was relatively dispersed. disco(2) mutant flies lacking the lateral neurons still showed an anticipatory activity, but with dispersed activity. These behavioural results suggest that not only LNs but also DNs and LPNs can, at least, partially participate in regulating the thermoperiodically induced rhythm.

    DOI: 10.1111/j.1460-9568.2005.04295.x

    Web of Science

    Scopus

    researchmap

  • Photoperiodic modulation of circadian rhythms in the cricket Gryllus bimaculatus 査読

    Koga, M., Ushirogawa, H., Tomioka, K.

    Journal of Insect Physiology   51 ( 6 SPEC. ISS. )   681 - 690   2005年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    The waveform and the free-running period of circadian rhythms in constant conditions are often modulated by preceding lighting conditions. We have examined the modulatory effect of variable length of light phase of a 24 h light cycle on the ratio of activity (a) and rest phase (p) as well as on the free-running period of the locomotor rhythm in the cricket Gryllus bimaculatus. When experienced the longer light phases, the alpha/p-ratio was smaller and the free-running period was shorter. The magnitude of changes in a/p-ratio was dependent on the number of cycles exposed, while the free-running period was changed by a single exposure, suggesting that there are separate regulatory mechanisms for the waveform and the free-running period. The neuronal activity of the optic lobe showed the a/p-ratio changing with the preceding photoperiod. When different photoperiodic conditions were given to each of the two optic lobe pacemakers, the a/p-ratio of a single pacemaker was rather intermediate between those of animals treated with either of the two conditions. These results suggest that the storage of the photoperiodic information occurs at least in part in the optic lobe pacemaker, and that the mutual interaction between the bilateral optic lobe pacemakers is involved in the photoperiodic modulation. (c) 2005 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.jinsphys.2005.01.006

    Web of Science

    Scopus

    researchmap

  • Drosophila cryb mutation reveals two circadian clocks that drive locomotor rhythm and have different responsiveness to light 査読

    Yoshii, T., Funada, Y., Ibuki-Ishibashi, T., Matsumoto, A., Tanimura, T., Tomioka, K.

    Journal of Insect Physiology   50 ( 6 )   479 - 488   2004年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Cryptochrome (CRY) is a blue-light-absorbing protein involved in the photic entrainment of the circadian clock in Drosophila melanogaster. We have investigated the locomotor activity rhythms of flies carrying cry(h) mutant and revealed that they have two separate circadian oscillators with different responsiveness to light. When kept in constant light conditions, wild-type flies became arrhythmic, while cry(b) mutant flies exhibited free-running rhythms with two rhythmic components, one with a shorter and the other with a longer free-running period. The rhythm dissociation was dependent on the light intensities: the higher the light intensities, the greater the proportion of animals exhibiting the two oscillations. External photoreceptors including the compound eyes and the ocelli are the likely photoreceptors for the rhythm dissociation, since rhythm dissociation was prevented in so(1);ery(h) and norpA(P41); cry(h) double mutant flies. Immunohistochemical analysis demonstrated that the PERIOD expression rhythms in ventrally located lateral neurons (LNvs) occurred synchronously with the shorter period component, while those in the dorsally located per-expressing neurons showed PER expression most likely related to the longer period component, in addition to that synchronized to the LNvs. These results suggest that the Drosophila locomotor rhythms are driven by two separate per-dependent clocks, responding differentially to constant light. (C) 2004 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.jinsphys.2004.02.011

    Web of Science

    Scopus

    researchmap

  • Circadian organization in hemimetabolous insects 査読

    Tomioka, K., Abdelsalam, S.

    Zoological Science   21 ( 12 )   1153 - 1162   2004年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

    The circadian system of hemimetabolous insects is reviewed in respect to the locus of the circadian clock and multioscillatory organization. Because of relatively easy access to the nervous system, the neuronal organization of the clock system in hemimetabolous insects has been studied, yielding identification of the compound eye as the major photoreceptor for entrainment and the optic lobe for the circadian clock locus. The clock site within the optic lobe is inconsistent among reported species; in cockroaches the lobula was previously thought to be a most likely clock locus but accessory medulla is recently stressed to be a clock center, while more distal part of the optic lobe including the lamina and the outer medulla area for the cricket. Identification of the clock cells needs further critical studies. Although each optic lobe clock seems functionally identical, in respect to photic entrainment and generation of the rhythm, the bilaterally paired clocks form a functional unit. They interact to produce a stable time structure within individual insects by exchanging photic and temporal information through neural pathways, in which serotonin and pigment-dispersing factor (PDF) are involved as chemical messengers. The mutual interaction also plays an important role in seasonal adaptation of the rhythm.

    DOI: 10.2108/zsj.21.1153

    Web of Science

    Scopus

    researchmap

  • Duration of development and number of nymphal instars are differentially regulated by photoperiod in the cricket Modicogryllus siamensis (Orthoptera: Gryllidae) 査読

    Taniguchi, N., Tomioka, K.

    European Journal of Entomology   100 ( 2 )   275 - 281   2003年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CZECH ACAD SCI, INST ENTOMOLOGY  

    The effect of photoperiod on nymphal development in the cricket Modicogryllus siamensis was studied. In constant long-days with 16 hr light at 25degreesC, nymphs matured within 40 days undergoing 7 moults, while in constant short-days with 12 hr light, 12-23 weeks and 11 or more moults were necessary for nymphal development. When nymphs were transferred from long to short day conditions in the 2nd instar, both the number of nymphal instars and the nymphal duration increased. However, only the nymphal duration increased when transferred to short day conditions in the 3rd instar or later. When the reciprocal transfer was made, the accelerating effect of long-days was less pronounced. The earlier the transfer was made, the fewer the nymphal instars and the shorter the nymphal duration. The decelerating effect of short-days or accelerating effect of long-days on nymphal development varied depending on instar. These results suggest that the photoperiod differentially controls the number of nymphal instars and the duration of each instar, and that the stage most important for the photoperiodic response is the 2nd instar.

    Web of Science

    Scopus

    researchmap

  • 5-HT7-like receptors mediate serotonergic modulation of photo-responsiveness of the medulla bilateral neurons in the cricket, Gryllus bimaculatus 査読

    Saifullah, A.S.M., Tomioka, K.

    Zoological Science   20 ( 3 )   303 - 309   2003年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

    Serotonin (5-HT) suppresses the photo-responsiveness of medulla bilateral neurons (MBNs) that are involved in the coupling mechanism of the bilaterally paired optic lobe circadian pacemakers in the cricket, Gryllus bimaculatus. We found that forskolin, a highly specific activator of adenylate cyclase, mimicked the effects of serotonin on the MBNs. This fact suggests the involvement of cyclic 3', 5'-adenosine monophosphate (cAMP) in mediating the action of serotonin. We therefore tested the effects of various 5-HT receptor agonists and antagonists that are coupled to adenylate cyclase to specify the receptor involved. Application of 8-OH-DPAT that has affinity for both 5-HT1A and 5-HT7 receptors suppressed the photo-responsiveness, like forskolin. The inhibitory effect of 8-OH-DPAT was effectively blocked by clozapine, a high affinity 5-HT7 receptor antagonists with a very low affinity for 5-HT2. Ketanserin, a selective 5-HT2 antagonist, and NAN-190, a 5-HT1A antagonist, did not block it. These results suggest that serotonergic suppression of the photo-responsiveness of the MBNs is mediated by 5-HT7-like receptor subtypes.

    DOI: 10.2108/zsj.20.303

    Web of Science

    Scopus

    researchmap

  • Pigment-dispersing factor sets the night state of the medulla bilateral neurons in the optic lobe of the cricket, Gryllus bimaculatus 査読

    Saifullah, A.S.M., Tomioka, K.

    Journal of Insect Physiology   49 ( 3 )   231 - 239   2003年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    Pigment-dispersing factor (PDF) is an octadeca-neuropeptide widely distributed in the insect brain and suggested to be involved in the insect circadian systems. We have examined its effects on the neuronal activity of the brain efferents in the optic stalk including medulla bilateral neurons (MBNs) in the cricket, Gryllus bimaculatus. The MBNs are visually responding interneurons connecting the bilateral medulla, which show a clear day/night change in their light responsiveness that is greater during the night. Microinjection of PDF into the optic lobe induced a significant increase in the spontaneous activity of the brain efferents and the photo-responsiveness of the MBNs during the day, while little change was induced during the night. The enhancing effects began to occur about 20 min after the injection and another 10 min was necessary to reach the maximal level. The effects of PDF were dose-dependent. When 22 nl of anti-Gryllus-PDF (1:200) IgG was injected into the medulla, the photo-responsiveness of the MBNs was suppressed in both the day and the night with greater magnitude during the night. No significant suppression was induced by injection of the same amount of IgG from normal rabbit serum. These results suggest that in the cricket optic lobe, PDF is released during the night and enhances MBNs' photo-responsiveness to set their night state. (C) 2003 Elsevier Science Ltd. All tights reserved.

    DOI: 10.1016/S0022-1910(02)00270-6

    Web of Science

    Scopus

    researchmap

  • Phase Shifts of the Circadian Locomotor Rhythm Induced by Pigment-Dispersing Factor in the Cricket Gryllus bimaculatus 査読

    Singaravel, M., Fujisawa, Y., Hisada, M., Saifullah, A.S.M., Tomioka, K.

    Zoological Science   20 ( 11 )   1347 - 1354   2003年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

    Pigment-dispersing factors (PDFs) are octadeca-peptides widely distributed in insect optic lobes and brain. In this study, we have purified PDF and determined its amino acid sequence in the cricket Gryllus bimaculatus. Its primary structure was NSEIINSLLGLPKVLNDA-NH2, homologous to other PDH family members so far reported. When injected into the optic lobe of experimentally blinded adult male crickets, Gryllus-PDF induced phase shifts in their activity rhythms in a phase dependent and dose dependent manner. The resulted phase response curve (PRC) showed delays during the late subjective night to early subjective day and advances during the mid subjective day to mid subjective night. The PRC was different in shape from those for light, serotonin and temperature. These results suggest that PDF plays a role in phase regulation of the circadian clock through a separate pathway from those of other known phase regulating agents.

    DOI: 10.2108/zsj.20.1347

    Web of Science

    Scopus

    researchmap

  • cDNA cloning and nuclear localization of the circadian neuropeptide designated as pigment-dispersing factor PDF in the cricket Gryllus bimaculatus 査読

    Chuman, Y., Matsushima, A., Sato, S., Tomioka, K., Tominaga, Y., Meinertzhagen, I.A., Shimohigashi, Y., Shimohigashi, M.

    Journal of Biochemistry   131 ( 6 )   895 - 903   2002年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPANESE BIOCHEMICAL SOC  

    Pigment-dispersing factor (PDF) was recently reported to be a principal circadian neuromodulator involved in transmitting circadian rhythms of daily locomotion in insects. In Drosophila, PDF functions in some of the neurons expressing the clock genes period, timeless, Clock, and cycle, and those clock genes in turn regulate pdf gene expression. In the present study, we cloned a cDNA encoding PDF in the brain of a nocturnal insect, the cricket Gryllus bimaculatus, and found that an isolated clone (310 bp) codes for an extraordinarily short precursor protein with no definite signal sequence, but a nuclear localization signal (NLS)-like sequence instead. The cricket PDF exhibits high sequence identity (78-94%) and similarity (89-100%) to insect PDFs and also to crustacean beta-PDH peptides. In the optic lobes of G. bimaculatus there are PDF-immunoreactive neurons in both the medulla and lamina neuropiles. Among the strongly immunoreactive lamina PDF neurons, on electron microscopy we identified cells exhibiting distinct staining that is not only cytoplasmic but also nuclear. When GFP-fused PDF precursor proteins were expressed in COS-7 cells, distinct translocation of the fusion protein into the nucleus was observed. This is the first finding of PDF peptide in the nucleus, which suggests a fundamental role of PDF peptide per se in the circadian clock system.

    Web of Science

    Scopus

    researchmap

  • Electrophysiological and morphological characterization of the medulla bilateral neurons that connect bilateral optic lobes in the cricket, Gryllus bimaculatus 査読

    Yukizane, M., Kaneko, A., Tomioka, K.

    Journal of Insect Physiology   48 ( 6 )   631 - 641   2002年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    The medulla bilateral neurons (MBNs) in the cricket brain directly connect two optic lobes and have been suggested to be involved in mutual coupling between the bilateral optic lobe circadian pacemakers. Single unit analysis with intracellular recording and staining with Lucifer Yellow was carried out to reveal morphology and physiology of the MBNs. Neurons having a receptive field in the rostral part of the compound eye showed greater response and a higher sensitivity to light than those having receptive fields in the ventro-caudal or dorsal portions. The MBN showed diurnal change in their responsiveness to light; the light-induced response in the night was about 1.3, 5 and 2 times of that in the day in MBN-1s, -3s and -4s, respectively. These results suggest that the MBNs mainly encode the temporal information by the magnitude of light-induced responses. The differences in magnitude of light-induced responses and of daily change in photo-responsiveness among MBNs may suggest that each group of MBNs plays different functional role in visual and/or circadian systems. (C) 2002 Elsevier Science Ltd. All rights reserved.

    DOI: 10.1016/S0022-1910(02)00091-4

    Web of Science

    Scopus

    researchmap

  • A temperature-dependent timing mechanism is involved in the circadian system that drives locomotor rhythms in the fruit fly Drosophila melanogaster 査読

    Yoshii, T., Sakamoto, M., Tomioka, K.

    Zoological Science   19 ( 8 )   841 - 850   2002年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

    The circadian clock of Drosophila melanogasteris thought to include rhythmic expression of period gene. Recent studies suggested, however, that a per-less oscillation is also involved in the regulation of circadian locomotor rhythms. In the present study, we examined the existence and the property of the possible per-less oscillation using arrhythmic clock mutant flies carrying per(01), tim(01), dClk(Jrk) or cyc(01), which lack rhythmic per expression. When temperature cycles consisting of 25 C and 30 C with various periods (T=8similar to32 hr) were given, wild-type (Canton-S) flies showed locomotor rhythms entrained to temperature cycles over a wide range of period (T=8similar to32 hr) in constant light (LL) while only to T=24 hr in constant darkness (DD). The mutant flies showed rhythms synchronizing with the given cycle both under LL and DD. In per(01) and tim(01) flies, the phase of a major peak slightly changed dependent on Ts in DD, while it did not in dClk(Jrk) and cyc(01) flies. When they were transferred from a constant temperature to a temperature cycle under DD, several cycles were necessary to establish a clear temperature entrainment in per(01) and tim(01) flies. These results suggest that per(01) and tim(01) flies have a temperature-entrainable weak oscillatory mechanism and that the per-less oscillatory mechanism may require dClk and cyc. In addition, per(01) and tim(01) flies changed from thermoactive in DD to cryoactive in LL, while dClk(Jrk) and cyc(01) flies did not. It is thus suggested that dClk and cyc are also involved in determining the light-associated temperature preference in per(01) and tim(01) flies.

    DOI: 10.2108/zsj.19.841

    Web of Science

    Scopus

    researchmap

  • Serotonin sets the day state in the neurons that control coupling between the optic lobe circadian pacemakers in the cricket Gryllus bimaculatus 査読

    Saifullah, A.S.M., Tomioka, K.

    Journal of Experimental Biology   205 ( 9 )   1305 - 1314   2002年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:COMPANY OF BIOLOGISTS LTD  

    The bilaterally paired optic lobe circadian pacemakers of the cricket Gryllus bimaculatus mutually exchange photic and circadian information to keep their activity synchronized. The information is mediated by a neural pathway, consisting of the so-called medulla bilateral neurons, connecting the medulla areas of the two optic lobes. We investigated the effects of serotonin on the neural activity in this coupling pathway. Spontaneous and light-induced electrical activity of the neurons in the coupling pathway showed daily variations, being more intense during the night than the day. Microinjection of serotonin or a serotonin-receptor agonist, quipazine, into the optic lobe caused a dose- and time-dependent inhibition of spontaneous and light-induced responses, mimicking the day state. The amount of suppression was greater and the recovery from the suppression occurred faster during the night. Application of metergoline, a non-selective serotonin-receptor antagonist, increased spontaneous activity and light-evoked responses during both the day and the night, with higher effect during the day. In addition, metergoline effectively attenuated the effects of serotonin. These facts suggest that in the cricket's optic lobe, serotonin is released during the daytime and sets the day state in the neurons regulating coupling between the bilaterally paired optic lobe circadian pacemakers.

    Web of Science

    Scopus

    researchmap

  • The role of the optic lobe in circadian locomotor rhythm generation in the cricket, Gryllus bimaculatus, with special reference to PDH-immunoreactive neurons 査読

    Okamoto, A., Mori, H., Tomioka, K.

    Journal of Insect Physiology   47 ( 8 )   889 - 895   2001年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    The role of optic lobes in rhythm generation was examined by a combination of surgical operation and immunohistochemistry using anti-beta -pigment-dispersing hormone (PDH) antibody. In adult male crickets, Gryllus bimaculatus, one optic lobe was removed and the contralateral optic lobe was surgically lesioned to varying extent. Locomotor rhythms persisted in most crickets with the optic nerve severed or with the lamina region removed, while they were lost when the lamina medulla complex was removed. The rhythm was also lost in all animals with the distal part of the medulla removed. In all cases, fragments of the PDH-immunoreactive neurons (PDH-IRNs) remained undegenerated in the brain, and in the animals with the medulla area partially removed, the shape of the PDH-IRNs showed a rough similarity to that in intact: animals, but the number of fibers projecting toward the cerebral lobe was increased. These results confirm our previous conclusion that the optic lobe is indispensable for the generation of locomotor rhythm. They also strongly suggest that the pacemaker resides in the medulla area or between the lamina and the medulla area, and that the PDH-IRNs in the medulla are not the pacemaker neurons. (C) 2001 Elsevier Science Ltd. All rights reserved.

    DOI: 10.1016/S0022-1910(01)00061-0

    Web of Science

    Scopus

    researchmap

  • Analysis of the mechanism underlying the rhythm reversal from diurnal to nocturnal in the cricket Gryllus bimaculatus, with special reference to the role of serotonin 査読

    Nishinokubi, I, K Tomioka

    ZOOLOGICAL SCIENCE   17 ( 8 )   1075 - 1080   2000年11月

     詳細を見る

    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

    The cricket, Gryllus bimaculatus, shows a rhythm reversal from diurnal to nocturnal in about a week after the imaginal molt. In the present study, we investigated the role of serotonin (5-HT) in the rhythm reversal. The 5-HT content in the brain measured by HPLC equipped with an electrochemical detector gradually increased after the imaginal molt, and in fully nocturnal adults it was about 2 times of nymphal level. We then examined the effects of 5,7-dihydroxytryptamine (5,7-DHT), a selective neurotoxine to serotonergic neurons, on the locomotor rhythm. In most animals with 5,7-DHT (25 muM or 250 muM, 32.2 nl) injected into the brain, daytime activity significantly increased even after the rhythm reversal, while nighttime activity was not significantly affected, forming rather diurnal pattern. The serotonin content in the brain of animals injected with 250 muM 5,7-DHT was reduced by about 30%. On the basis of these results, possible involvement of 5-HT in the neural mechanism controlling the locomotor rhythm is discussed.

    DOI: 10.2108/zsj.17.1075

    Web of Science

    researchmap

  • Protein synthesis is a required process for the optic lobe circadian clock in the cricket Gryllus bimaculatus 査読

    Tomioka, K.

    Journal of Comparative Physiology   46 ( 3 )   281 - 287   2000年

     詳細を見る

    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/S0022-1910(99)00181-X

    Web of Science

    Scopus

    researchmap

  • Light and serotonin phase shifts the optic lobe circadian pacemaker in vitro in the cricket Gryllus bimaculatus. 査読

    Tomioka, K

    Journal of Comparative Physiology   A185(5),437-444   1999年

     詳細を見る

    担当区分:筆頭著者, 責任著者   記述言語:英語  

    researchmap

  • tim(rit) Lengthens circadian period in a temperature-dependent manner through suppression of PERIOD protein cycling and nuclear localization 査読

    Matsumoto, A., Tomioka, K., Chiba, Y., Tanimura, T.

    Molecular and Cellular Biology   19 ( 6 )   4343 - 4354   1999年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    A fundamental feature of circadian clocks is temperature compensation of period. The free-running period of ritsu (tim(rit)) (a novel allele of timeless [tim]) mutants is drastically lengthened in a temperature-dependent manner. PER and TIM protein levels become lower in tim(rit) mutants as temperature becomes higher. This mutation reduces per mRNA but not tim mRNA abundance. PER constitutively driven by the rhodopsin1 promoter is lowered in rit mutants, indicating that tim(rit) mainly affects the per feedback loop at a posttranscriptional level. An excess of per+ gene dosage can ameliorate all rit phenotypes, including the weak nuclear localization of PER, suggesting that tim(rit) affects circadian rhythms by reducing PER abundance and its subsequent transportation into nuclei as temperature increases.

    DOI: 10.1128/MCB.19.6.4343

    Scopus

    PubMed

    researchmap

  • Light and serotonin phase-shift the circadian clock in the cricket optic lobe in vitro 査読

    Tomioka, K.

    Journal of Comparative Physiology - A Sensory, Neural, and Behavioral Physiology   185 ( 5 )   437 - 444   1999年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER VERLAG  

    Light and serotonin were found to cause phase shifts of the circadian neural activity rhythm in the optic lobe of the cricket Gryllus bimaculatus cultured in vitro. The two phase-shifting agents yielded phase-response curves different in shape. Light induced phase delay and advance in the early and late subjective night, respectively, and almost no shifts in the subjective day, whereas serotonin phase-advances the clock during the subjective day and induced delay shifts during the subjective:night. The largest phase advance and delay occurred at circadian time 21 and 12, respectively, for light, and circadian time 3 and 18, respectively, for serotonin. Quipazine, a nonspecific serotonin agonist, induced phase advance and phase delay at circadian time 3 and 18, respectively, like serotonin. (+/-)8-OH-DPAT, a specific 5-HT1A agonist, phase delayed by 2 h at the subjective night, but produced no significant phase shifts at the subjective day. When NAN-190, a specific 5-HT1A antagonist, was applied together with quipazine, it completely blocked the phase delay at circadian time 18, whereas it had no effect on the advance shifts induced by quipazine. The results suggest that the phase dependency of serotonin-induced phase shifts of the clock may be partly attributable to the daily change in receptor type.

    Web of Science

    Scopus

    researchmap

  • Effects of 5,7-DHT injection into the optic lobe on the circadian locomotor rhythm in the cricket, Gryllus bimaculatus 査読

    M Germ, K Tomioka

    ZOOLOGICAL SCIENCE   15 ( 3 )   317 - 322   1998年6月

     詳細を見る

    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

    The effect of direct 5,7-dihydroxytryptamine (5,7-DHT) injection into the medulla region of the optic lobe on the locomotor activity was investigated in the adult male cricket, Gryllus bimaculatus. After a 6 hr phase advance of a light-dark cycle, the 5,7-DHT injected animals needed significantly longer time for resynchronization to the new cycle (6.55 +/- 0.62 days) than the control, Ringer's solution injected animals (3.17 +/- 0.15 days; P < 0.001, t-test). Light induced a bout of activity (i.e., masking effect) when light-dark cycle was phase advanced by 6 hr and the duration of the masking effect was significantly longer in 5,7-DHT injected animals. An initial bout of the nocturnal activity was significantly greater in the 5,7-DHT injected animal. Under constant darkness, the freerunning periods of both groups were not significantly different. Under constant light, a significantly higher percentage of 5,7-DHT injected animals showed arrhythmicity compared with the control group. An analysis carried by high-pressure liquid chromatography with electrochemical detection (HPLC-ECD) revealed that the serotonin content in the optic robe was significantly reduced to less than 50% in the 5,7-DHT injected animals, even one month after the injection. These results suggest that serotonin plays important roles in the regulation of circadian locomotor rhythms of the cricket mainly by regulating the sensitivity of the photoreceptive system.

    DOI: 10.2108/zsj.15.317

    Web of Science

    Scopus

    researchmap

  • Light and temperature cooperate to regulate the circadian locomotor rhythm of wild type and period mutants of Drosophila melanogaster 査読

    Tomioka, K., Sakamoto, M., Harui, Y., Matsumoto, N., Matsumoto, A.

    Journal of Insect Physiology   44 ( 7-8 )   587 - 596   1998年

     詳細を見る

    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In the wild type (Canton-S) and period mutant flies of Drosophila melanogaster, we examined the effects of light and temperature on the circadian locomotor rhythm. Under light dark cycles, the wild type and per(s) flies were diurnal at 25°C. However, at 30°C, the daytime activity commonly decreased to form a rather nocturnal pattern, and ultradian rhythms of a 2 ~ 4 h period were observed more frequently than at 25°C. The change in activity pattern was more clearly observed in per(o) flies, suggesting that these temperature dependent changes in activity pattern are mainly attributable to the system other than the circadian clock. In a 12 h 30°C:12 h 25°C temperature cycle (HTLT12:12), per(o) flies were active during the thermophase in constant darkness (DD) but during the cryophase in constant light (LL). The results of experiments with per(o)
    eya flies suggest that the compound eye is the main source of the photic information for this reversal. Wild type and per(o) flies were synchronized to HTLT12:12 both under LL and DD, while per(S) and per(L) flies were synchronized only in LL. This suggests that the circadian clock is entrainable to the temperature cycle, but the entrainability is reduced in the per(S) and per(L) flies to this particular thermoperiod length, and that temperature cycle forces the clock to move in LL, where the rhythm is believed to be stopped at constant temperature.

    DOI: 10.1016/S0022-1910(98)00046-8

    Scopus

    researchmap

  • Circadian period modulation and masking effects induced by short light pulses in locomotor rhythms of the cricket, Gryllus bimaculatus. 査読

    Germ, M., Tomioka, K.

    Zoological Science   15 ( 3 )   309 - 316   1998年

     詳細を見る

    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2108/zsj.15.309

    Web of Science

    Scopus

    researchmap

  • The optic lobe: a component of the circadian timekeeping mechanism in insects. 査読

    Tomioka K

    Trends in Comparative Biochemistry and Physiology   5, 161-169   161 - 169   1998年

     詳細を見る

    担当区分:筆頭著者   記述言語:英語  

    researchmap

  • Light cycles given during development affect freerunning period of circadian locomotor rhythm of period mutants in Drosophila melanogaster 査読

    Tomioka, K., Uwozumi, K., Matsumoto, N.

    Journal of Insect Physiology   43 ( 3 )   297 - 305   1997年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We reared wild type (Canton-S) and period mutant flies, i.e., per(S) and per(L), of Drosophila melanogaster in constant darkness, constant light or 24h light dark cycles with various light to dark ratios throughout the development from embryo to early adult. The locomotor activity rhythms of newly eclosed individuals were subsequently monitored in the lighting conditions, in which they had been reared, for several days and then in constant darkness. Circadian rhythms were clearly exhibited in constant darkness even in flies reared in constant light and constant darkness as well as flies reared in light-dark cycles, but the freerunning period differed among groups. The results suggest that the circadian clock is assembled without any cyclical photic information, and that the light influences the developing circadian clock of Drosophila to alter the freerunning period. The effects of light on the rhythm differed in some aspects between per(L) flies and the other two strains. Possible mechanisms through which light affects the developing circadian clock are discussed.

    DOI: 10.1016/S0022-1910(96)00075-3

    Scopus

    researchmap

  • A specific area of the compound eye in the cricket Gryllus bimaculatus sends photic information to the circadian pacemaker in the contralateral optic lobe 査読

    Tomioka, K., Yukizane, M.

    Journal of Comparative Physiology - A Sensory, Neural, and Behavioral Physiology   180 ( 1 )   63 - 70   1997年

     詳細を見る

    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The circadian locomotor rhythm of the cricket Gryllus bimaculatus is primarily regulated by a pair of interacting optic lobe circadian pacemaker systems. The interaction involves phase-dependent modulation of the free- running period and phase-dependent suppression of activity. Since photic information has been shown to be involved in the interaction, we examined the regional difference in photoreception for the interaction within cricket compound eyes. The activity rhythm of animals receiving partial reduction of one compound eye combined with severance of the contralateral optic nerve split into entrained and free-running components under a 13-h light to 13-h dark cycle. All the animals operated on showed a phase-dependent suppression of activity, and most animals showed a phase-dependent modulation of the period of the free-running component. However, removal of the dorsocaudal area of the compound eye resulted in a severe reduction of the amplitude of the phase-dependent-period modulation. These results suggest that the dorsocaudal portion of the compound eye is a specific region receiving photic signals that are transmitted to the circadian pacemaker in the contralateral optic lobe and that the phase-dependent suppression of activity is caused by a mechanism separate from that for the period modulation.

    DOI: 10.1007/s003590050027

    Scopus

    researchmap

  • Circadian locomotor rhythms in the cricket, Gryllodes sigillatus II. Interactions between bilaterally paired circadian pacemakers 査読

    Ushirogawa, H., Abe, Y., Tomioka, K.

    Zoological Science   14 ( 5 )   1997年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    Scopus

    researchmap

  • Circadian locomotor rhythms in the cricket, Gryllodes sigillatus I. Localization of the pacemaker and the photoreceptor 査読

    Abe, Y., Ushirogawa, H., Tomioka, K.

    Zoological Science   14 ( 5 )   1997年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    Scopus

    researchmap

  • Electrical properties of the cerebral prothoracicotropic hormone cells in diapausing and non-diapausing pupae of the tobacco hornworm, Manduca sexta 査読

    K. Tomioka, N. Agui, W. E. Bollenbacher

    Zoological Science   12 ( 2 )   165 - 173   1995年

     詳細を見る

    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Zoological Society of Japan  

    DOI: 10.2108/zsj.12.165

    Scopus

    PubMed

    researchmap

  • ritsu: A rhythm mutant from a natural population of drosophila melanogaster 査読

    Murata, T., Matsumoto, A., Tomioka, K., Chiba, Y.

    Journal of Neurogenetics   9 ( 4 )   239 - 249   1995年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.3109/01677069509084160

    Scopus

    researchmap

  • Neural pathways involved in mutual interactions between optic lobe circadian pacemakers in the cricket Gryllus bimaculatus 査読

    Yukizane, M., Tomioka, K.

    Journal of Comparative Physiology A   176 ( 5 )   1995年

     詳細を見る

    担当区分:責任著者   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/BF00192489

    Scopus

    researchmap

  • Variability in circadian activity patterns within the Culex pipiens complex (Diptera, Culicidae) 査読

    Shinkawa, Y., Takeda, S.I., Tomioka, K., Matsumoto, A., Oda, T., Chiba, Y.

    Journal of Medical Entomology   31 ( 1 )   49 - 56   1994年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    Scopus

    researchmap

  • Chronobiological analysis of a new clcock mutant Toki in Drosophila melanogaster. 査読

    Matsumoto, A., Motoshige, T., Murata, T., Tomioka, K., Tanimura, T., Chiba, Y.

    Journal of Neurogenetics   9 ( 3 )   141 - 155   1994年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3109/01677069409167276

    Scopus

    researchmap

  • Optic lobe circadian pacemaker sends its information to the contralateral optic lobe in the cricket Gryllus bimaculatus 査読

    Tomioka, K., Nakamichi, M., Yukizane, M.

    Journal of Comparative Physiology A   175 ( 4 )   381 - 388   1994年

     詳細を見る

    担当区分:筆頭著者, 責任著者   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/BF00199246

    Scopus

    researchmap

  • Involvement of serotonin in the circadian rhythm of an insect visual system(共著) 査読

    K. Tomioka, M. Ikeda, T. Nagao, S. Tamotsu

    Naturwissenshaften   80 ( 3 )   137 - 139   1993年3月

     詳細を見る

    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/BF01131019

    Scopus

    researchmap

  • Analysis of postembryonic development of locomotor activity rhythm by corpora allata implantation in the cricket Gryllus bimaculatus 査読

    Tomioka, K., Seto, N., Okada, S.-I., Terada, T., Chiba, Y.

    Comparative Biochemistry and Physiology   106 ( 1 )   1993年

     詳細を見る

    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/0300-9629(93)90036-4

    Scopus

    researchmap

  • Temperature dependency of the circadian locomotor rhythm in the cricket Gryllus bimaculatus 査読

    Ikeda, M, Tomioka, K

    Zoological Science   10   597 - 604   1993年

     詳細を見る

    担当区分:最終著者, 責任著者   記述言語:英語  

    researchmap

  • Entrainability of circadian activity of the mosquito Culex pipiens pallens to 24-hr temperature cycles, with special reference to involvement of multiple oscillators. 査読

    Chiba, Y., Uki, M., Kawasaki, Y., Matsumoto, A., Tomioka, K.

    Journal of Biological Rhythms   8 ( 3 )   211 - 220   1993年

     詳細を見る

    担当区分:最終著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1177/074873049300800304

    Scopus

    PubMed

    researchmap

  • Analysis of coupling between optic lobe circadian pacemakers in the cricket Gryllus bimaculatus 査読

    Tomioka, K.

    Journal of Comparative Physiology A   172 ( 4 )   1993年

     詳細を見る

    担当区分:筆頭著者   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/BF00213522

    Scopus

    researchmap

  • Characterization of an optic lobe circadian pacemaker by in situ and in vitro recording of neural activity in the cricket, Gryllus bimaculatus 査読

    Tomioka, K., Chiba, Y.

    Journal of Comparative Physiology A   171 ( 1 )   1992年

     詳細を見る

    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/BF00195955

    Scopus

    researchmap

  • Entrainability of diphasic circadian activity of the mosquito Culex pipiens molestus to 24-hour light dark cycles : a significance of critical light-to-dark ratio. 査読

    Chiba, Y, Tomioka, K

    Zoological Science   9   185 - 192   1992年

     詳細を見る

    担当区分:最終著者   記述言語:英語  

    researchmap

  • Mutual communication between optic lobes and constant light affect developing circadian system in the cricket, Gryllus bimaculatus 査読

    Tomioka, K., Chiba, Y.

    Comparative Biochemistry and Physiology -- Part A: Physiology   102 ( 2 )   253 - 258   1992年

     詳細を見る

    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/0300-9629(92)90130-I

    Scopus

    researchmap

  • A comparative study on insemination dependency of circadian activity pattern in mosquitoes 査読

    YOSHIHIKO CHIBA, YASUHIRO SHINKAWA, MAKOTO YOSHII, AKIRA MATSUMOTO, KENJI TOMIOKA, SUSUMU Y. TAKAHASHI

    Physiological Entomology   17 ( 3 )   213 - 218   1992年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Abstract. Two forms of mosquitoes in the Culex pipiens complex, C.p.pallens and C.p.molestus, were compared for the insemination‐dependency of entrained female circadian activity in LD 16:8h. When virgin, Pallens is nocturnal and molestus is, in addition, diurnal. Insemination and injection of extracts of the male accessory gland affects virgin activity in that nocturnality is strengthened in both mosquitoes, and the diurnal activity of molestus is depressed. This was so whether the treatments were intra‐ or interspecific. These findings are compared with those already published for other mosquitoes. A coherent view is proposed that the mosquito, nocturnal/crepuscular, nocturnal and diurnal, or diurnal, has potentially a similar entrained waveform which is affected by insemination in a common way, and by lighting conditions in species specific ways. Copyright © 1992, Wiley Blackwell. All rights reserved

    DOI: 10.1111/j.1365-3032.1992.tb01013.x

    Scopus

    researchmap

  • Mutual interactions between optic lobe circadian pacemakers in the cricket Gryllus bimaculatus 査読

    Tomioka, K., Yamada, K., Yokoyama, S., Chiba, Y.

    Journal of Comparative Physiology A   169 ( 3 )   291 - 298   1991年

     詳細を見る

    担当区分:筆頭著者, 責任著者   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/BF00206993

    Scopus

    researchmap

  • Circadian control of hatching in the cricket, Gryllus bimaculatus 査読

    Tomioka, K., Wakatsuki, T., Shimono, K., Chiba, Y.

    Journal of Insect Physiology   37 ( 5 )   365 - 371   1991年

     詳細を見る

    担当区分:筆頭著者, 責任著者  

    DOI: 10.1016/0022-1910(91)90087-G

    Scopus

    researchmap

  • Circadian phase-response curves for light in nymphal and adult crickets, Gryllus bimaculatus 査読

    Okada, Y., Tomioka, K., Chiba, Y.

    Journal of Insect Physiology   37 ( 8 )   583 - 590   1991年

     詳細を見る

    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    The circadian locomotor rhythm of the cricket, Gryllus bimaculatus, is regulated by two pacemakers, one located in each optic lamina-medulla complex of the protocerebrum, and is readily entrained to light-dark cycles. We investigated effects of light pulses on nymphal (diurnal) and adult (nocturnal) free-running locomotor rhythms. When a 3 h light pulse was given, adult crickets free-running under constant darkness showed a clear phase shift in a manner dependent on time of day. The phase-response curves for the light pulses showed phase delays in the early subjective night, phase advances in the late subjective night, and little response in the mid-subjective day. Unilateral removal of the lamina-medulla-compound eye complex had no effects on phase-responsiveness to 3 h light pulses, suggesting that the shape of the phase-response curve reflects the property of a single circadian pacemaker. The average free-running period for the operated animal [23.73 +/- 0.25 (SD) h, N = 58] was significantly shorter than that of the intact animal [23.86 +/- 0.24 (SD) h, N = 99] (t-test, P < 0.01), suggesting the existence of mutual interaction between the optic lobe pacemakers. Eighth-instar nymphal crickets were revealed to have a phase-response curve similar to that of adults, although their circadian rhythm is quite different from that of adults not only in phasing but also in free-running period and waveform. The fact strongly suggests that the adult nocturnal and the nymphal diurnal rhythms share common pacemakers.

    DOI: 10.1016/0022-1910(91)90035-X

    Web of Science

    Scopus

    researchmap

  • Development of circadian sleep-wakefulness rhythmicity of three infants 査読

    Tomioka, K, Tomioka, F

    Journal of Interdisciplinary Cycle Research   22   71 - 80   1991年

     詳細を見る

    担当区分:筆頭著者, 責任著者   記述言語:英語  

    researchmap

  • Insemination dependent modification of circadian activity of the mosquito Culex pipiens pallens. 査読

    Chiba, Y, Yamamoto, Y, Shimizu, C, Zaitsu, M, Uki, M, Yoshii, M, Tomioka, K

    Zoological Science   7   895 - 906   1990年

     詳細を見る

    担当区分:最終著者  

    researchmap

  • Distribution of Circadian Photoreceptors in the Compound Eye of the Cricket Gryllus bimaculatus 査読

    Tomioka, K., Okada, Y., Chiba, Y.

    Journal of Biological Rhythms   5 ( 4 )   131 - 139   1990年

     詳細を見る

    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1177/074873049000500403

    Scopus

    researchmap

  • Photoperiod during post-embryonic develeopment affects some parameters of adult circadian rhythm in the cricket, Gryllus bimaculatus. 査読

    Tomioka, K, Chiba, Y

    Zoological Science   6   565 - 571   1989年

     詳細を見る

    担当区分:筆頭著者, 責任著者   記述言語:英語  

    researchmap

  • Light cycle during post-embryonic development affects adult circadian parameters of the cricket (Gryllus bimaculatus) optic lobe pacemaker 査読

    Tomioka, K., Chiba, Y.

    Journal of Insect Physiology   35 ( 4 )   273 - 276   1989年

     詳細を見る

    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    DOI: 10.1016/0022-1910(89)90074-7

    Web of Science

    Scopus

    researchmap

  • Photoperiodic entrainment of locomotor activity in crickets (Gryllus bimaculatus) lacking the optic lobe pacemaker 査読

    Tomioka, K., Chiba, Y.

    Journal of Insect Physiology   35 ( 11 )   827 - &   1989年

     詳細を見る

    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    DOI: 10.1016/0022-1910(89)90098-X

    Web of Science

    Scopus

    researchmap

  • NEUROPHYSIOLOGICAL EVENTS DURING THE HEAD CRITICAL PERIOD FOR PROTHORACICOTROPIC HORMONE-RELEASE IN 14TH-INSTAR LARVAE OF MANDUCA-SEXTA 査読

    K TOMIOKA, WE BOLLENBACHER

    JOURNAL OF INSECT PHYSIOLOGY   35 ( 12 )   1023 - 1030   1989年

     詳細を見る

    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    DOI: 10.1016/0022-1910(89)90026-7

    Web of Science

    Scopus

    researchmap

  • ENTRAINMENT OF CRICKET CIRCADIAN ACTIVITY RHYTHM AFTER 6-HOUR PHASE-SHIFTS OF LIGHT-DARK CYCLE 査読

    K TOMIOKA, Y CHIBA

    ZOOLOGICAL SCIENCE   4 ( 3 )   535 - 542   1987年6月

     詳細を見る

    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

    Web of Science

    researchmap

  • Circadian rhythm in the neurally isolated lamina-medulla-complex of the cricket, Gryllus bimaculatus 査読

    Tomioka, K., Chiba, Y.

    Journal of Insect Physiology   32 ( 9 )   1986年

     詳細を見る

    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/0022-1910(86)90077-6

    Scopus

    researchmap

  • OPTIC LOBE-COMPOUND EYE SYSTEM IN CRICKET - A COMPLETE CIRCADIAN SYSTEM 査読

    K TOMIOKA, Y CHIBA

    JOURNAL OF INTERDISCIPLINARY CYCLE RESEARCH   16 ( 1 )   73 - 76   1985年

     詳細を見る

    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SWETS ZEITLINGER PUBLISHERS  

    Web of Science

    researchmap

  • Residual circadian rhythmicity after bilateral lamina-medulla removal or optic stalk transection in the cricket, Gryllus bimaculatus 査読

    Tomioka, K.

    Journal of Insect Physiology   31 ( 8 )   653 - 657   1985年

     詳細を見る

    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    DOI: 10.1016/0022-1910(85)90065-4

    Web of Science

    Scopus

    researchmap

  • Effects of nymphal stage optic nerve severance or optic lobe removal on the circadian locomotor rhythm of the cricket, Gryllus bimaculatus. 査読

    Tomioka, K, Chiba, Y

    Zoological Science   1   375 - 382   1984年

     詳細を見る

    担当区分:筆頭著者, 責任著者   記述言語:英語  

    researchmap

  • Response modification of cricket sensory interneurons during flight. 査読

    Tomioka, K, Yamaguchi, T

    Zoological Science   1   169 - 186   1984年

     詳細を見る

    担当区分:筆頭著者   記述言語:英語  

    researchmap

  • フタホシコオロギ(Gryllus bimaculatus)の頸部および胸部における筋肉・神経系の機能解剖. 査読

    古川直哉, 富岡憲治, 山口恒夫

    Zoological Magazine   92   371 - 385   1983年

     詳細を見る

  • Post-embryonic development of circadian rhythm in the cricket, Gryllus bimaculatus: A rhythm reversal 査読

    Tomioka, K., Chiba, Y.

    Journal of Comparative Physiology □ A   147 ( 3 )   299 - 304   1982年

     詳細を見る

    担当区分:筆頭著者   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/BF00609663

    Scopus

    researchmap

  • Persistence of circadian ERG rhythm in the cricket with optic tract severed 査読

    Tomioka, K., Chiba, Y.

    Naturwissenschaften   69 ( 8 )   395 - 396   1982年

     詳細を見る

    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    DOI: 10.1007/BF00396696

    Web of Science

    Scopus

    researchmap

  • Steering responses of adult and nymphal crickets to light, with special reference to the head rolling movement 査読

    Tomioka, K., Yamaguchi, T.

    Journal of Insect Physiology   26 ( 1 )   47 - 57   1980年

     詳細を見る

    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

    DOI: 10.1016/0022-1910(80)90109-2

    Web of Science

    Scopus

    researchmap

▼全件表示

書籍等出版物

  • 生物学辞典

    東京化学同人  2010年 

     詳細を見る

  • 動物の多様な生き方1 見える光,見えない光、動物と光のかかわり、

    共立出版  2009年 

     詳細を見る

  • 昆虫はスーパー脳

    技術評論社  2008年 

     詳細を見る

  • 時間生物学事典

    朝倉書店  2008年 

     詳細を見る

  • 時間生物学事典

    朝倉書店  2008年 

     詳細を見る

  • 昆虫ミメティクス

    エヌ・ティー・エス  2008年 

     詳細を見る

  • シリーズ21世紀の動物科学第9巻 動物の感覚とリズム

    2007年 

     詳細を見る

  • 時間生物学の基礎

    裳華房  2003年 

     詳細を見る

  • 昆虫学大辞典

    朝倉書店  2003年 

     詳細を見る

  • Circadian Clock as Multi-Oscillator System

    Hokkaido University Press  2003年 

     詳細を見る

  • 情報学事典

    弘文堂  2002年 

     詳細を見る

  • 時間と時

    学会出版センター  2002年 

     詳細を見る

  • Insect Timing: Circadian Rhythmicity to Seasonality.

    Elsevier Science BV  2001年 

     詳細を見る

  • シリーズ・光が拓く生命科学、第一巻生物の光環境センサー

    79-92  1999年 

     詳細を見る

  • 生物時計の分子生物学

    シュプリンガー・フェアラーク東京  1999年 

     詳細を見る

  • Circadian activity rhythm of the cricket

    Modern Animal Ethology. Vol. 4 Behavioral Rhythm and Learning, Sangyo Tosho 

     詳細を見る

  • Circadian activity rhythm of the cricket

    Modern Animal Ethology. Vol. 4 Behavioral Rhythm and Learning, Sangyo Tosho 

     詳細を見る

  • Control of circadian rhythmicity by interacting two optic lobe pacemakers in crickets.

    Circadian Clocks from Cell to Human. Hokkaido Univ. Press 

     詳細を見る

  • 時間を知る生物

    裳華房 

     詳細を見る

  • 概日時計は脳にある

    昆虫の脳を探る。共立出版 

     詳細を見る

  • 研究法

    時間生物学ハンドブック、朝倉書店 

     詳細を見る

  • 概日リズムの神経内分泌機構、昆虫

    時間生物学ハンドブック、朝倉書店 

     詳細を見る

  • 3-2動物の概日時計「シリーズ・光が拓く生命科学、第一巻生物の光環境センサー」

    津田基之編、共立出版 

     詳細を見る

  • 昆虫の視葉時計:海老原史樹文・深田吉孝編:生物時計の分子生物学

    シュプリンガー・フェラーク東京 

     詳細を見る

  • 動物材料各論・コオロギ

    動物遺伝学実験法 共立出版 

     詳細を見る

  • コオロギの概日活動

    現代の動物行動学 第4巻 行動のリズムと学習、 

     詳細を見る

  • Control of circadian rhythmicity by interacting two optic lobe pacemakers in crickets.

    Circadian Clocks from Cell to Human. Hokkaido Univ. Press 

     詳細を見る

  • Methodology for Chronobiology

    Handbook of Chronobiology. Asakura Shoten 

     詳細を見る

  • Neuroendocrine regulation of insect development.

    Biomechanisms Regulating Grwoth and Development. Kluwer Academic Press 

     詳細を見る

  • Physiological mechanisms of entrainment of mosquito and cricket circadian activities of light-dark cycle.

    Circadian Clock and Zeitgebers. Hokkaido Univ. Press 

     詳細を見る

  • Neural control of circadian rest-activity rhythm in crickets.

    Endogenous Sleep Substances and Sleep Regulation. VNS Science Press 

     詳細を見る

  • Organisms knowing time

    Shokabo 

     詳細を見る

  • Methodology for Chronobiology

    Handbook of Chronobiology. Asakura Shoten 

     詳細を見る

  • Neural and endocrine control of insect circadian rhythm

    Handbook of Chronobiology. Asakura Shoten 

     詳細を見る

  • Crickets as an experimental animals for genetics

    Methods for animal genetics, Kyorits Shuppan. 

     詳細を見る

  • Physical and chemical properties of a male secretory factor modifying female circadian pattern in the mosquito Culex pipiens pallens.

    Host Regulated Developmental Mechanism in Vector Arthropods. Univ. Florida Press 

     詳細を見る

  • Circadian clock is in the brain

    Investigating the Insect Brain 

     詳細を見る

  • Circadian clock is in the brain

    Investigating the Insect Brain 

     詳細を見る

  • Neuroendocrine regulation of insect development.

    Biomechanisms Regulating Grwoth and Development. Kluwer Academic Press 

     詳細を見る

  • Physiological mechanisms of entrainment of mosquito and cricket circadian activities of light-dark cycle.

    Circadian Clock and Zeitgebers. Hokkaido Univ. Press 

     詳細を見る

  • Neural control of circadian rest-activity rhythm in crickets.

    Endogenous Sleep Substances and Sleep Regulation. VNS Science Press 

     詳細を見る

  • Organisms knowing time

    Shokabo 

     詳細を見る

  • Neural and endocrine control of insect circadian rhythm

    Handbook of Chronobiology. Asakura Shoten 

     詳細を見る

  • Crickets as an experimental animals for genetics

    Methods for animal genetics, Kyorits Shuppan. 

     詳細を見る

  • Physical and chemical properties of a male secretory factor modifying female circadian pattern in the mosquito Culex pipiens pallens.

    Host Regulated Developmental Mechanism in Vector Arthropods. Univ. Florida Press 

     詳細を見る

▼全件表示

MISC

  • コオロギを実験動物とした生物時計の解析:時計遺伝子peirodのリズム発現機構における機能解析

    坂本智昭, 守山禎之, 富岡憲治

    岡山実験動物研究会報   ( 25 )   7 - 11   2009年

     詳細を見る

  • キイロショウジョウバエ概日時計の温度サイクル同調機構

    吉井大志, 富岡憲治

    時間生物学   13   13 - 19   2007年

     詳細を見る

  • Effects of per dsRNA on the circadian locomotor rhythm in the cricket Gryllus bimaculatus

    Yoshiyuki Moriyama, Tomoaki Sakamoto, Kenji Tomioka

    ZOOLOGICAL SCIENCE   23 ( 12 )   1193 - 1193   2006年12月

     詳細を見る

    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

    Web of Science

    researchmap

  • Multioscillatory control of locomotor activity in the cricket, Gryllus bimaculatus: Evidences for a circadian oscillator outside the optic lobes

    Karpova Svetlana, Kenji Tomioka

    ZOOLOGICAL SCIENCE   23 ( 12 )   1193 - 1193   2006年12月

     詳細を見る

    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

    Web of Science

    researchmap

  • Clock genes controlling locomotor rhythms are involved in the circadian cuticle growth rhythm in Drosophila melanogaster

    Chihiro Ito, Shut G. Goto, Hideharu Numata, Kenji Tomioka

    ZOOLOGICAL SCIENCE   23 ( 12 )   1193 - 1193   2006年12月

     詳細を見る

    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

    Web of Science

    researchmap

  • Analysis of multiocsillatory system driving locomotor rhythm of Drosophila melanogaster

    Yujirou Umezaki, Kenji Tomioka

    ZOOLOGICAL SCIENCE   23 ( 12 )   1193 - 1193   2006年12月

     詳細を見る

    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

    Web of Science

    researchmap

  • Neuronal mechanisms for photic and thermoperiodic entrainment of Drosophila circadian locomotor rhythms

    Yoko Miyasako, Kenji Tomioka

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY   145 ( 3-4 )   415 - 415   2006年11月

     詳細を見る

    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ELSEVIER SCIENCE INC  

    DOI: 10.1016/j.cbpb.2006.10.054

    Web of Science

    researchmap

  • Temperature steps induce anticipatory locomotor activity of the circadian clock under constant light in Drosophila melanogaster

    Taishi Yoshii, Kenji Tomioka

    JOURNAL OF NEUROGENETICS   20 ( 3-4 )   263 - 264   2006年7月

     詳細を見る

    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Web of Science

    researchmap

  • cDNA cloning of clock genes in two species of crickets Gryllus bimaculatus and Modicogryllus siamensis

    Tomoaki Sakamoto, Abdelsalam Salaheldin, Taishi Yoshii, Akira Matsumoto, Kenji Tomioka

    ZOOLOGICAL SCIENCE   22 ( 12 )   1490 - 1490   2005年12月

     詳細を見る

    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

    Web of Science

    researchmap

  • Postembryonic changes in circadian rhythms of visual interneurons in the cricket, Gryllus bimaculatus

    Hiroyuki Uemura, Kenji Tomioka

    ZOOLOGICAL SCIENCE   21 ( 12 )   1324 - 1324   2004年12月

     詳細を見る

    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

    Web of Science

    researchmap

  • Entrainment of circadian clocks in insects: Roles of light and temperature

    Kenji Tomioka

    ZOOLOGICAL SCIENCE   21 ( 12 )   1235 - 1235   2004年12月

     詳細を見る

    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

    Web of Science

    researchmap

  • Circadian chances in PDF content in the optic lobe and the brain of the cricket, Gryllus bimaculatus

    Salaheldin Abdelsalam, Kenji Tomioka

    ZOOLOGICAL SCIENCE   21 ( 12 )   1323 - 1323   2004年12月

     詳細を見る

    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

    Web of Science

    researchmap

  • 複数振動体系による昆虫概日リズムの構築

    富岡憲治

    比較生理生化学   20:140-146   2003年

  • Photoperiodic modulation of insect circadian rhythms.

    Tomioka K, Uwozumi K, Koga M

    Journal of Photoscience   9, 9-12   2002年

     詳細を見る

  • Pigment-dispersing factor induces phase shifts of circadian locomotor rhythm in the cricket Gryllus bimaculatus.

    Singaravel M, Tomioka K

    Journal of Photoscience   2002年

     詳細を見る

  • Possible involvement of temperature-entrainable timing system in arrhythmic mutant flies in Drosophila melanogaster.

    Yoshii T, Tomioka K

    Journal of Photoscience   2002年

     詳細を見る

  • Gene Expression of a Circadian Pacemaker Hormone Peptide PDF in Cricket Gryllus bimaculatus,

    Matsushima, A, Chuman, Y, Onoue, H, Ito, Y, Tomioka, K, Tominaga, Y, Shimohigashi, Y, Shimohigashi M

    Peptide Science 2000   2001年

     詳細を見る

  • キイロショウジョウバエ概日活動の恒明・温度サイクル下での振動機構の解析

    藤村幸子, 伊吹正, 松本顕, 谷村禎一, 富岡憲治

    日本動物学会中国四国支部会報   ( 52 )   23   2000年5月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • 生物時計の動きの機構--昆虫での知見

    富岡憲治

    脳の科学   22, 513-517   2000年

     詳細を見る

  • ショウジョウバエ時計突然変異体Tokiとritsuの遺伝学的解析

    松本顕, 富岡憲治, 千葉喜彦, 谷村禎一

    日本分子生物学会年会プログラム・講演要旨集   22nd   250   1999年11月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • キイロショウジョウバエ概日時計の恒明下での温度サイクルによる再駆動

    富岡 憲治, 阪本 真, 松本 顕, 谷村 禎一

    日本時間生物学会会誌: Journal of Chronobiology   5 ( 2 )   41 - 41   1999年10月

     詳細を見る

  • キイロショウジョウバエにおける概日リズムの温度依存的発現の組織特異性

    富岡憲治, 阪本真, 松本顕, 谷村禎一

    日本動物学会大会予稿集   70th   31   1999年9月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • キイロショウジョウバエ概日時計温度感受性の組織特異性

    富岡憲治, 阪本真, 松本顕, 谷村禎一

    日本動物学会中国四国支部会報   ( 51 )   3   1999年5月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • キイロショウジョウバエper突然変異系統における概日活動の温度周期同調性の解析

    阪本真, 松本顕, 谷村禎一, 富岡憲治

    日本動物学会大会予稿集   69th   125   1998年9月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • 特集にあたって、生物時計の重要性を考える

    富岡憲治

    遺伝   52, 13-14   1998年

     詳細を見る

  • キイロショウジョウバエ概日時計長周期系統ritsuの周期の変動性

    松本顕, 富岡憲治, 千葉喜彦

    日本動物学会大会予稿集   64th   128   1993年11月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • キイロショウジョウバエper<sup>0</sup>系統における活動の光及び温度依存性

    治井有加, 松本顕, 富岡憲治, 千葉喜彦

    日本動物学会大会予稿集   64th   128   1993年11月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • ショウジョウバエ概日時計の遺伝学的研究

    千葉喜彦, 谷村禎一, 富岡憲治, 松本顕

    日産科学振興財団研究報告書   14(1991)   159 - 167   1992年2月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • 112 アカイエカCulex pipiens pallens概日活動の温度依存性に関する推計学的研究

    千葉 喜彦, 松本 顕, 富岡 憲治

    日本生気象学会雑誌   28 ( 3 )   39 - 39   1991年

     詳細を見る

    記述言語:日本語   出版者・発行元:日本生気象学会  

    DOI: 10.11227/seikisho1966.28.3_39

    CiNii Article

    researchmap

▼全件表示

講演・口頭発表等

  • コオロギ概日時計の光同調機構

    OKOIS昆虫学研究会  2017年 

     詳細を見る

  • タンボコオロギ幼虫発育の光周制御機構の検討

    日本動物学会中国四国支部大会  2017年 

     詳細を見る

  • 繁殖期におけるブッポウソウの警戒行動とモビングについて

    日本動物学会第88回大会  2017年 

     詳細を見る

  • フタホシコオロギにおける誘引歌および日周発音行動への時計遺伝子の関与

    日本動物学会第88回大会  2017年 

     詳細を見る

  • Timeout is involved in the circadian rhythm and reproduction in the cricket Gryllus bimaculatus

    15th Congress of European Biological Rhythms Society  2017年 

     詳細を見る

  • Cryptochrome genes form and oscillatory loop that can operate independent of the per/tim loop in the cricket Gryllus bimaculatus.

    15th Congress of European Biological Rhythms Society  2017年 

     詳細を見る

  • Timeless2 plays an important role in the circadian clock of the cricket, Gryllus bimaculatus.

    第39回日本比較生理生化学会大会  2017年 

     詳細を見る

  • Molecular physiological mechanism of nymphal diapause in the cricket Modicogryllus siamensis.

    日本時間生物学会学術大会  2017年 

     詳細を見る

  • Pittendrighの古典を読む:ショウジョウバエの時計機構を中心に.

    日本時間生物学会 時間生物学トレーニングコース  2017年 

     詳細を見る

  • タンボコオロギ幼虫発育の光周制御機構の検討

    日本動物学会第88回大会  2017年 

     詳細を見る

  • タンボコオロギ幼虫発育の光周制御機構の検討

    OKOIS昆虫学研究会  2017年 

     詳細を見る

  • タンボコオロギ幼虫発育を制御する光周測時機構における光誘導相の検討

    日本動物学会中四国支部大会  2014年 

     詳細を見る

  • 平成26年度日本比較生理生化学会賞受賞者講演,昆虫の概日時計機構の比較生理学的研究

    日本比較生理生化学会第36回大会  2014年 

     詳細を見る

  • ショウジョウバエ概日リズムを制御する時計ニューロンネットワーク

    第2回視交叉上核アリーナ  2014年 

     詳細を見る

  • フタホシコオロギ時計関連遺伝子vrilleの概日時計機構における機能の解析

    日本動物学会中四国支部大会  2014年 

     詳細を見る

  • Molecular dissection of the circadian clock system in the cricket

    Symposium on Molecular Clock 2014. Epigenetic Landscape in Biological Rhythms  2014年 

     詳細を見る

  • Molecular basis on tissue regeneration in the cricket as revealed by RNA interference

    International Symposium on RNAi and Genome Editing Methods  2014年 

     詳細を見る

  • The opsin-LW plays an important role in light entrainment of the circadian clock in the cricket, Gryllus bimaculatus

    International Symposium on RNAi and Genome Editing Methods  2014年 

     詳細を見る

  • Molecular approach to the circadian clock that controls locomotor rhythms in the cricket Gryllus bimaculatus

    Hokkaido Neuroethology Workshops 2014, Ethology, neuroscience and genetics in crickets: How can they meet?  2014年 

     詳細を見る

  • Role of opsins in circadian and photoperiodic systems in crickets

    The 30th Anniversary Meeting of Sapporo Symposium on Biological Rhythm  2014年 

     詳細を見る

  • キイロショウジョウバエchico, Fmr1, cycleのPDF細胞特異的発現抑制の概日活動リズム加齢変化への影響

    日本動物学会中四国支部大会  2014年 

     詳細を見る

  • フタホシコオロギ概日時計の振動機構の解析

    第20回日本時間生物学会学術大会  2013年 

     詳細を見る

  • マダラシミ概日リズムの分子振動機構

    日本動物学会第84回大会  2013年 

     詳細を見る

  • 平成25年度日本動物学会賞受賞者講演,昆虫の概日時計機構に関する神経生物学的研究

    日本動物学会第84回大会  2013年 

     詳細を見る

  • フタホシコオロギ概日時計関連遺伝子cry1, cry2の機能解析

    日本動物学会中四国支部大会  2013年 

     詳細を見る

  • キイロショウジョウバエ視覚系変異系統と視葉欠損系統での羽化リズム

    日本動物学会第84回大会  2013年 

     詳細を見る

  • キイロショウジョウバエ概日時計のCRY依存的な光同調神経機構

    日本動物学会第84回大会  2013年 

     詳細を見る

  • フタホシコオロギ時計遺伝子E75,HR3の機能解析

    日本動物学会第84回大会  2013年 

     詳細を見る

  • フタホシコオロギ複眼オプシンによる概日時計の光同調

    日本動物学会第84回大会  2013年 

     詳細を見る

  • 昆虫のサーカディアンリズムを制御する体内時計機構

    生化学若い研究者の会中四国支部主催、生命科学秋セミナー  2013年 

     詳細を見る

  • キイロショウジョウバエ活動リズムの加齢変化へのPDFの関与

    日本動物学会中四国支部大会  2012年 

     詳細を見る

  • Molecular characterization and functional analysis of the clock gene cycle in the cricket Gryllus bimaculatus

    SRBR 2012  2012年 

     詳細を見る

  • Sexual interaction influences circadian activity pattern in Drosophila melanogaster.

    SRBR 2012  2012年 

     詳細を見る

  • Functional analysis of the clock gene Clock in the cricket Gryllus bimaculatus

    SRBR 2012  2012年 

     詳細を見る

  • フタホシコオロギ概日活動リズムの光同調におけるオプシン類の役割

    日本動物学会中四国支部大会  2012年 

     詳細を見る

  • Molecular dissection of cricket’s clock systems

    XI Latin American Symposium on Chronobiology  2011年 

     詳細を見る

  • Molecular dissection of an insect circadian clock withy RNAi

    International Congress of Comparative Physiology and Biochemistry 2011  2011年 

     詳細を見る

  • フタホシコオロギ色素拡散因子の概日時計振動機構への影響

    日本動物学会中四国支部大会  2011年 

     詳細を見る

  • Post-embryonic development of the circadian rhythms outside the optic lobe in the cricket, Gryllus bimaculatus.

    International Congress of Comparative Physiology and Biochemistry 2011  2011年 

     詳細を見る

  • Functional analysis of the clock genes in the bristle tail, Thermobia domestica.

    International Congress of Comparative Physiology and Biochemistry 2011  2011年 

     詳細を見る

  • Circadian clock regulates physiological functions through a neuropeptide PDF in the cricket Gryllus bimaculatus

    International Congress of Comparative Physiology and Biochemistry 2011  2011年 

     詳細を見る

  • キイロショウジョウバエ雌雄個体間相互作用による概日リズムの変調機構

    日本動物学会中四国支部大会  2011年 

     詳細を見る

  • A possible synaptic transmission involved in the Drosophila circadian clock

    3rd World Congress of Chronobiology  2011年 

     詳細を見る

  • コオロギ概日時計の光リセット機構の転写レベルでの解析

    第17回日本時間生物学会学術大会  2010年 

     詳細を見る

  • フタホシコオロギ視葉外組織における時計遺伝子発現リズムの後胚発生

    日本動物学会第81回大会  2010年 

     詳細を見る

  • フタホシコオロギ活動リズム制御におけるPDFの役割

    日本動物学会第81回大会  2010年 

     詳細を見る

  • キイロショウジョウバエ概日リズムの加齢変化への色素拡散因子の(PDF)の関与の検討

    日本動物学会第81回大会  2010年 

     詳細を見る

  • マダラシミ時計遺伝子のcDNAクローニングとRNA干渉による機能解析

    第17回日本時間生物学会学術大会  2010年 

     詳細を見る

  • フタホシコオロギ雌活動リズムの交尾による変調機構

    第17回日本時間生物学会学術大会  2010年 

     詳細を見る

  • フタホシコオロギ視葉外組織での時計遺伝子 period, timeless mRNA発現の時間的制御

    日本動物学会第80回大会  2009年 

     詳細を見る

  • フタホシコオロギ時計機構におけるtimelessとcryptochrome2の機能解析

    日本時間生物学会第16回学術大会  2009年 

     詳細を見る

  • Functional analysis of the clock genes period and Clock with RNA interference in the cricket Gryllus bimaculatus

    International Symposium on Biological Rhythm Joint Symposium on Photic Bioimaging  2009年 

     詳細を見る

  • Analysis of synaptic outputs of PDF-expressing circadian clock neurons in the fruit fly

    International Symposium on Biological Rhythm Joint Symposium on Photic Bioimaging  2009年 

     詳細を見る

  • Dissection of the cricket circadian system with molecular tools

    International Symposium on Biological Rhythm Joint Symposium on Photic Bioimaging  2009年 

     詳細を見る

  • フタホシコオロギ脳におけるPDFニューロンの後胚発生

    日本動物学会第61回中国四国支部大会  2009年 

     詳細を見る

  • コオロギの場合―日周リズムと季節への適応

    日本動物学会、日本植物学会、日本生態学会、生物系三学会中国四国支部大会、公開シンポジウム  2009年 

     詳細を見る

  • フタホシコオロギ脳・腹部最終神経節および末梢組織における時計遺伝子period, timeless mRNA発現の時間的制御

    日本動物学会第61回中国四国支部大会  2009年 

     詳細を見る

  • キイロショウジョウバエ体内時計機構の寿命への影響

    日本時間生物学会第16回学術大会  2009年 

     詳細を見る

  • キイロショウジョウバエのクチクラ形成リズムの温度同調機構

    日本時間生物学会第16回学術大会  2009年 

     詳細を見る

  • RNA intereference reveals the role of PDF in the circadian rhythym of the cricket Gryllus bimaculatus

    第34回日本比較生理生化学会大会  2009年 

     詳細を見る

  • マダラシミ時計遺伝子Clockの発現リズムとRNA干渉による機能解析

    日本動物学会第80回大会  2009年 

     詳細を見る

  • Molecular dissection of insect circadian systems.

    AQUA-TERR, School of Biological Sciences, Madurai Kamaraj University  2009年 

     詳細を見る

  • Phase regulation of the Drosophila circadian system by light and temperature

    XI. Congress of the European Biological Rhythms Society  2009年 

     詳細を見る

  • フタホシコオロギ概日時計の胚発生の検討

    第34回日本比較生理生化学会大会  2009年 

     詳細を見る

  • フタホシコオロギClock遺伝子の概日時計機構における機能解析

    第34回日本比較生理生化学会大会  2009年 

     詳細を見る

  • キイロショウジョウバエ概日時計機構への加齢の影響

    日本動物学会第80回大会  2009年 

     詳細を見る

  • 時計遺伝子RNA干渉によるフタホシコオロギperiod遺伝子の機能解析

    第79回日本動物学会大会  2008年 

     詳細を見る

  • period gene plays a role in circadian rhythm and photoperiodism in crickets.

    XXIII International Congress of Entomology  2008年 

     詳細を見る

  • Molecular investigation of roles of a clock gene period in circadian rhythm generation and photoperiodism in crickets.

    Special lecture, Suez Canal University  2008年 

     詳細を見る

  • フタホシコオロギ時計遺伝子timelessの発現リズムと機能解析

    第79回日本動物学会大会  2008年 

     詳細を見る

  • キイロショウジョウバエのクチクラ形成リズムの温度周期への同調

    第15回日本時間生物学会学術大会  2008年 

     詳細を見る

  • キイロショウジョウバエ概日時計ニューロンのシナプス出力の解析

    第15回日本時間生物学会学術大会  2008年 

     詳細を見る

  • マダラシミ時計遺伝子timelessとClockのcDNAクローニング

    第79回日本動物学会大会  2008年 

     詳細を見る

  • キイロショウジョウバエにおける雌雄の相互作用の寿命と活動リズムへの影響

    第15回日本時間生物学会学術大会  2008年 

     詳細を見る

  • Analysis of photo-entrainability of circadian oscillators driving locomotor activity rhythms in Drosophila melanogaster.

    国際シンポジウム Comprehensive understanding of diverse biological timing mechanism  2007年 

     詳細を見る

  • Clock gene period plays a role in photoperiodic time-measurement in the cricket, Modicogryllus siamensis

    国際シンポジウム Comprehensive understanding of diverse biological timing mechanism  2007年 

     詳細を見る

  • Molecular dissection of the circadian clock in crickets.

    Special Lecture, University of Marburg  2007年 

     詳細を見る

  • The circadian system of Drosophila: entrainment by light and temperature.

    Special Seminar, University of Regensburg  2007年 

     詳細を見る

  • The Drosophila circadian system: entrainment by light and temperature

    11th Symposium on Invertebrate Neurobiology  2007年 

     詳細を見る

  • After-effects of temperature entrainment on the circadian locomotor rhythm in the cricket Gryllus bimaculatus

    日本比較生理生化学会第29回大会  2007年 

     詳細を見る

  • Brain measures day length.

    International Symposium “Time in Brain”  2007年 

     詳細を見る

  • Analysis of the role of synaptic output in circadian pacemaker neurons in Drosophila melanogaster.

    国際シンポジウム Comprehensive understanding of diverse biological timing mechanism  2007年 

     詳細を見る

  • キイロショウジョウバエの活動リズムを制御する複数の体内時計の光応答性の解析

    第14回日本時間生物学会学術大会  2007年 

     詳細を見る

  • period plays a role in photoperiodic control of nymphal development in the cricket Modicogryllus siamensis

    第2回世界時間生物学会 シンポジウム Photoperiodism in insects and vertebrates  2007年 

     詳細を見る

  • Effects of unilateral compound eye removal on the photoperiodic responses of nymphal development in the cricket Modicogryllus siamensis.

    国際シンポジウム Comprehensive understanding of diverse biological timing mechanism  2007年 

     詳細を見る

  • RNA interference of a clock gene period stops the circadian clock in adult crickets Gryllus bimaculatus.

    国際シンポジウム Comprehensive understanding of diverse biological timing mechanism  2007年 

     詳細を見る

  • Molecular dissection of the circadian clock in crickets.

    Special Lecture, University of Regensburg  2007年 

     詳細を見る

  • キイロショウジョウバエ概日活動リズムを制御する時計機構の階層性

    日本比較生理生化学会第29回大会  2007年 

     詳細を見る

  • キイロショウジョウバエの活動リズムを制御する複数の体内時計の光応答性の解析

    日本動物学会中国四国支部大会  2007年 

     詳細を見る

  • フタホシコオロギ視葉概日時計の脳内出力経路の解析

    日本動物学会中国四国支部大会  2007年 

     詳細を見る

  • 時計遺伝子RNA干渉によるフタホシコオロギ成虫の概日時計の停止

    日本動物学会第78回大会  2007年 

     詳細を見る

  • 時計遺伝子periodのRNA干渉によるタンボコオロギ光周反応の阻害

    日本動物学会第78回大会  2007年 

     詳細を見る

  • ショウジョウバエ概日時計の光及び温度同調

    日本動物学会第78回大会成茂記念動物科学シンポジウム「感覚の分子生理学の最前線」  2007年 

     詳細を見る

  • キイロショウジョウバエの複数振動体の解析

    第77回日本動物学会大会  2006年 

     詳細を見る

  • フタホシコオロギper遺伝子のRNA干渉による活動リズムの抑制

    日本比較生理生化学会第28回大会  2006年 

     詳細を見る

  • キイロショウジョウバエ概日リズムの温度依存的振動機構の解析

    第77回日本動物学会大会  2006年 

     詳細を見る

  • ショウジョウバエクチクラ形成リズムへの概日時計遺伝子の関与

    第77回日本動物学会大会  2006年 

     詳細を見る

  • キイロショウジョウバエ歩行活動リズムを制御する複数振動体系の解析

    2006年度中国四国動物生理シンポジウム  2006年 

     詳細を見る

  • キイロショウジョウバエ概日リズムの光周期および温度周期への同調機構

    2006年度中国四国動物生理シンポジウム  2006年 

     詳細を見る

  • Effect of optic lobectomy on the thermoperiodic entrainment of circadian locomotor activity rhythm in the cricket Gryllus bimaculatus

    日本動物学会中国四国支部大会  2006年 

     詳細を見る

  • The Drosophila circadian system: photoperiodic and thermoperiodic entrainment.

    国際生物学賞記念シンポジウム  2006年 

     詳細を見る

  • Entrainment of circadian locomotor rhythm to temperature cycles in the cricket

    第13回日本時間生物学会学術大会  2006年 

     詳細を見る

  • コオロギ時計遺伝子のクローニングと機能探索

    第13回日本時間生物学会学術大会  2006年 

     詳細を見る

  • フタホシコオロギ歩行活動リズムを駆動する視葉外概日振動体

    第77回日本動物学会大会  2006年 

     詳細を見る

  • フタホシコオロギper dsRNA投与の活動リズムへの影響

    第77回日本動物学会大会  2006年 

     詳細を見る

  • タンボコオロギ概日活動リズムの光周期による変調

    第77回日本動物学会大会  2006年 

     詳細を見る

  • Temperature entrainment of circadian locomotor rhythm in cricket (Gryllus bimaculatus) after removal of optic lobes.

    日本比較生理生化学会第28回大会  2006年 

     詳細を見る

  • フタホシコオロギ時計遺伝子のRNA干渉による活動リズムの抑制

    2006年度中国四国動物生理シンポジウム  2006年 

     詳細を見る

  • キイロショウジョウバエ歩行活動リズムの光および温度同調の神経機構

    日本比較生理生化学会第28回大会  2006年 

     詳細を見る

  • Temperature cycles drive behavioral and molecular circadian rhythms under constant light that otherwise induces arrhythmicity in Drosophila melanogaster

    Neurobioloogy of Drosophila  2005年 

     詳細を見る

  • キイロショウジョウバエのクチクラ形成概日リズムにおけるper遺伝子の役割

    第12回日本時間生物学会大会  2005年 

     詳細を見る

  • 審査するものとされるもの(「若手研究(A)」を獲得するために)

    日本比較生理生化学会若手の会「夏の集い」  2005年 

     詳細を見る

  • Circadian changes in PDF in the optic lobe and the brain of the last instar nymphs and adults of the cricket Gryllus bimaculatus

    日本動物学会中国四国支部大会  2005年 

     詳細を見る

  • マダラシミ(Thermobia domestica)における歩行活動リズムおよび能の組織学的解析

    日本動物学会中国四国支部大会  2005年 

     詳細を見る

  • Photoperiodic control of nymphal deveopment in the cricket, Modicogryllus siamensis

    Taiwan-Japan-Czech Republic Cooperation Symposium on Entomology  2005年 

     詳細を見る

  • フタホシコオロギ視葉視覚性介在ニューロン群の光応答に対するPDFの影響

    日本比較生理生化学会第27回大会  2005年 

     詳細を見る

  • フタホシコオロギPDF発現の日周変動の解析

    日本比較生理生化学会第27回大  2005年 

     詳細を見る

  • フタホシコオロギ視葉色素拡散ホルモン様ペプチドmRNA発現の日周性の解析

    日本動物学会第76回大会  2005年 

     詳細を見る

  • コオロギ時計遺伝子のクローニングと発現解析

    日本動物学会第76回大会  2005年 

     詳細を見る

  • キイロショウジョウバエのクチクラ形成における概日リズムの光同調機構

    日本動物学会第76回大会  2005年 

     詳細を見る

  • コオロギの概日リズムと季節適応

    日本動物学会第76回大会 関連集会  2005年 

     詳細を見る

  • Drosophila circadian locomotor rhythm is driven by clocks with different responsiveness to light and temperature

    1st Aschoff-Honma Memorial Symposium  2005年 

     詳細を見る

  • 昆虫の概日リズム機構

    大阪大学蛋白質研究所セミナー「体内時計と体内恒常性維持機構」  2005年 

     詳細を見る

  • ショウジョウバエ概日活動リズムの光と温度による制御

    日本動物学会第76回大会シンポジウム「概日時計の振動機構と機能分化」  2005年 

     詳細を見る

  • キイロショウジョウバエにおける光および温度同調性時計の探索

    日本動物学会第76回大会  2005年 

     詳細を見る

  • キイロショウジョウバエ概日時計の温度周期による駆動機構の解析

    第12回日本時間生物学会大会  2005年 

     詳細を見る

  • エンマコオロギ後胚発生の光周制御

    日本動物学会中国四国支部大会  2004年 

     詳細を見る

  • フタホシコオロギ視葉および脳におけるPDF量の日周変動

    第75回日本動物学会大会  2004年 

     詳細を見る

  • 昆虫における概日時計の時刻あわせ:光と温度の効果

    第75回日本動物学会大会、シンポジウム3D「生物時計の時刻あわせ」  2004年 

     詳細を見る

  • Thermoperiodic restoration of Drosophila circadian rhythms in constant light

    22nd International Congress of Entomology  2004年 

     詳細を見る

  • クリプトクロムノックダウンによるキイロショウジョウバエ概日時計機構の解析

    第11回日本時間生物学会大会  2004年 

     詳細を見る

  • キイロショウジョウバエ概日時計の温度ステップによる恒明下での駆動

    第75回日本動物学会大会  2004年 

     詳細を見る

  • フタホシコオロギ視覚性介在ニューロン群の概日リズムの後胚発生

    第75回日本動物学会大会  2004年 

     詳細を見る

  • タンボコオロギ幼虫発育の光周反応に対する片側複眼切除の影響

    第75回日本動物学会大  2004年 

     詳細を見る

  • フタホシコオロギ視葉視覚性介在ニューロン群の概日光応答リズムの後胚発生における変化

    日本動物学会中国四国支部大会  2004年 

     詳細を見る

  • 昆虫の概日リズムを制御する複数振動体系

    第198回 昆虫学土曜セミナー  2004年 

     詳細を見る

  • キイロショウジョウバエ概日リズムを制御する複数振動体系

    日本動物学会岡山県例会  2004年 

     詳細を見る

  • Photoperiodic modulation of circadian rhythms in crickets

    22nd International Congress of Entomology  2004年 

     詳細を見る

  • エンマコオロギ概日リズムと後胚発生の光周制御

    第26回日本比較生理生化学会大  2004年 

     詳細を見る

  • Circadian locomotor rhythm of Drosophila cryb mutant flies is driven by two clocks with different responsiveness to light

    14TH International Congress on Photobiology  2004年 

     詳細を見る

  • Drosophila cryb mutant flies show two period-based circadian oscillations with different responsiveness to constant light

    1st World Congress of Chronobiology  2003年 

     詳細を見る

  • 昆虫の体内時計による光環境情報の記憶

    平成14年度JST異分野研究者交流フォーラム 昆虫科学-小さな生物の機能がもつ大きな可能性-  2003年 

     詳細を見る

  • キイロショウジョウバエcryb突然変異系統の活動リズム分裂に関る光受容器の探索

    日本動物学会第74回大会  2003年 

     詳細を見る

  • コオロギ視髄両側性ニューロン群の光応答抑制に関るセロトニン受容体

    日本比較生理生化学会第25回大会  2003年 

     詳細を見る

  • キイロショウジョウバエにおける恒明・温度周期下でのTIMLESSおよびPEIRODの発現

    日本動物学会中四国支部大会  2003年 

     詳細を見る

  • 5-HTPによるフタホシコオロギ概日活動リズムの位相変位

    日本動物学会中四国支部大会  2003年 

     詳細を見る

  • フタホシコオロギ(Gryllus bimaculatus)における概日活動リズムの温度同調性

    日本動物学会中四国支部大会  2003年 

     詳細を見る

  • キイロショウジョウバエcryb突然変異系統の2つの概日リズムの光と温度への同調性

    日本動物学会第74回大会  2003年 

     詳細を見る

  • コオロギ視髄両側性ニューロンの光応答の概日時計制御

    第6回光生物シンポジウム  2003年 

     詳細を見る

  • Two weakly coupled clocks driving the circadian locomotor rhythm in crickets

    1st World Congress of Chronobiology  2003年 

     詳細を見る

  • A phase response curve for pigment dispersing factor in the cricket Gryllus bimaculatus.

    2nd Czech-Japan seminar on Entomology  2002年 

     詳細を見る

  • キイロショウジョウバエ概日時計機構におけるPDFの役割

    日本動物学会中四国支部大会  2002年 

     詳細を見る

  • タンボコオロギ(Modicogryllus siamensis)後胚発生の光周制御

    日本時間生物学会大会  2002年 

     詳細を見る

  • Effects of pigment-dispersing factor and serotonin on the circadian locomotor rhythm in the cricket Gryllus bimaculatus

    日本時間生物学会大会  2002年 

     詳細を見る

  • Photoperiodic modulation of circadian rhythms in the crickets Gryllus bimaculatus.

    2nd Czech-Japan seminar on Entomology  2002年 

     詳細を見る

  • Pigment dispersing factor induces phase shifts of circadian locomotor rhythm in the cricket Gryllus bimaculatus

    1st Asian Conference of Photobiology  2002年 

     詳細を見る

  • Possible involvement of temperature-entrainable timing system in arrhythmic mutant flies in Drosophila melanogaster

    1st Asian Conference of Photobiology  2002年 

     詳細を見る

  • Photoperiodic modulation of insect circadian rhythms.

    1st Asian Conference of Photobiology  2002年 

     詳細を見る

  • キイロショウジョウバエ概日時計の温度周期による再駆動機構の解析

    日本動物学会第73回大会  2002年 

     詳細を見る

  • キイロショウジョウバエ概日時計機構における複数振動体の組織学的解析

    日本動物学会第73回大会  2002年 

     詳細を見る

  • フタホシコオロギ概日系におけるPDFの機能解析

    日本動物学会第73回大会  2002年 

     詳細を見る

  • キイロショウジョウバエ無周期突然変位系統を用いた温度依存性測時系の解析

    日本動物学会中国四国支部大会  2001年 

     詳細を見る

  • Serotonergic modulation of MBNs electrical responses is mediated by 5-HT receptors linked to adenylate cyclase in the cricket, Gryllus bimaculatus

    日本時間生物学会学術大会  2001年 

     詳細を見る

  • キイロショウジョウバエ温度依存性測時系の解析

    日本動物学会第72回大会  2001年 

     詳細を見る

  • Involvement of serotonin and PDF in the circadian pacemaker coupling of the cricket Gryllus bimaculatus.

    9th Sapporo Symposium on Biological Rhythm  2001年 

     詳細を見る

  • Multioscillator systems controlling the circadian locomotor rhythm in insects.

    9th Sapporo Symposium on Biological Rhythm  2001年 

     詳細を見る

  • Serotonin controls the circadian pacemaker coupling in the cricket, Gryllus bimaculatus.

    Central Europian Conference of Neurobiology  2001年 

     詳細を見る

  • キイロショウジョウバエ活動リズムを制御する2つの概日時計

    日本動物学会第72回大会  2001年 

     詳細を見る

  • ショウジョウバエにおけるChAT発現ニューロンの分布変化が概日リズムに及ぼす影響

    日本動物学会第72回大会  2001年 

     詳細を見る

  • コオロギ視柄内遠心性ニューロン群電気的応答へのセロトニンとPDFの影響

    日本動物学会第72回大会  2001年 

     詳細を見る

  • 日周リズムと光

    日本動物学会第72回大会関連集会光生物シンポジウム「環境としての光と生物――地球規模で生物を考えよう!」  2001年 

     詳細を見る

  • 光周期によるコオロギ視葉概日時計の波形変調

    日本比較生理生化学会第12回大会  2001年 

     詳細を見る

  • 複数振動体における時間的秩序の確立

    日本比較生理生化学会第12回大会  2001年 

     詳細を見る

  • フタホシコオロギ色素拡散ホルモン様ペプチドの単離と抗体作製

    日本比較生理生化学会第11回大会  2000年 

     詳細を見る

  • コオロギ視髄両側性ニューロン光応答性のセロトニンによる修飾

    日本動物学会中四国支部大会  2000年 

     詳細を見る

  • Involvement of serotonergic system in the coupling between the optic lobe circadian pacemakers in crickets.

    International Conference, Complex Clocks: Light, Circadian rhythms and Photoperiodism  2000年 

     詳細を見る

  • キイロショウジョウバエ概日活動の恒明・温度サイクル下での振動機構の解析

    日本動物学会中四国支部大会  2000年 

     詳細を見る

  • フタホシコオロギ概日時計機構の光周反応

    日本動物学会第71回大会  2000年 

     詳細を見る

  • フタホシコオロギ視髄両側性ニューロンにおけるセロトニンによる光応答修飾の解析

    日本比較生理生化学会第11回大会  2000年 

     詳細を見る

  • フタホシコオロギ概日リズムの光周期による変調機構の解析

    日本比較生理生化学会第11回大会  2000年 

     詳細を見る

  • コオロギ視髄両側性ニューロンの光応答性のセロトニンによる修飾

    日本動物学会第71回大会  2000年 

     詳細を見る

  • Protein synthesis is required for circadian rhythm generation in the optic lobe of the cricket Gryllus bimaculatus.

    International Congress of Entomology  2000年 

     詳細を見る

  • フタホシコオロギの概日リズムペースメーカーホルモンPDHのcDNAクローニングと遺伝子発現

    日本比較生理生化学会第11回大会  2000年 

     詳細を見る

  • キイロショウジョウバエ概日時計温度感受性の組織特異性

    日本動物学会中四国支部大会  1999年 

     詳細を見る

  • フタホシコオロギ成虫化に伴うリズム逆転におけるセロトニンの役割

    日本動物学会中四国支部大会  1999年 

     詳細を見る

  • D-perと昆虫の体内時計機構

    大阪大学蛋白質研究所セミナー「体内時計の分子機構ー研究の最前線ー」  1999年 

     詳細を見る

  • キイロショウジョウバエ概日時計の恒明下での温度による再駆動

    日本時間生物学会第6回学術大会  1999年 

     詳細を見る

  • 生物時計---環境への調和の手段

    林原フォーラム「時間と時」  1999年 

     詳細を見る

  • キイロショウジョウバエChAT cDNA形質転換体の概日活動の解析

    日本動物学会中四国支部大会  1999年 

     詳細を見る

  • フタホシコロオギ成虫化に伴う活動リズム逆転におけるセロトニンの役割

    日本動物学会第70回大会  1999年 

     詳細を見る

  • キイロショウジョウバエにおける概日リズムの温度依存的発現の組織特異性

    日本動物学会第70回大会  1999年 

     詳細を見る

  • The neural mechanism of circadian timekeeping in the cricket.

    International Congress of Comparative Physiology and Biochemisrty  1999年 

     詳細を見る

  • キイロショウジョウバエper突然変異系統における概日活動の温度周期同調性の解析

    日本動物学会中国四国支部大会  1998年 

     詳細を見る

  • 昆虫概日系の生理学的解析

    名古屋大学大学院人間情報学研究科シンポジウム「生物リズム研究の展望−最新の分子的研究の成果をもとに−」  1998年 

     詳細を見る

  • フタホシコオロギ視葉PDH-免疫陽性線維の概日時計機構への関与

    日本時間生物学会大会  1998年 

     詳細を見る

  • コオロギ視葉概日時計に対する蛋白合成阻害剤の影響

    日本時間生物学会大会  1998年 

     詳細を見る

  • キイロショウジョウバエper突然変異系統における概日活動の温度周期同調性の解析

    日本動物学会第69回大会  1998年 

     詳細を見る

  • シクロヘキシミドによるコオロギ視葉概日時計の位相変位

    日本動物学会第69回大会  1998年 

     詳細を見る

  • キイロショウジョウバエ概日時計同調系へのコリン作動性ニューロンの関与

    日本比較生理生化学会第9回大会  1998年 

     詳細を見る

  • シクロヘキシミドによるコオロギ視葉概日時計のリセット

    日本比較生理生化学会第9回大会  1998年 

     詳細を見る

  • コオロギ視葉ニューロンの成虫脱皮に伴う脳内投射様式の変化:電位感受性色素を利用した光学測定法による解析

    日本動物学会中国四国支部大会  1998年 

     詳細を見る

▼全件表示

Works(作品等)

  • 体内時計の仕組みを探る

    2007年

     詳細を見る

    作品分類:芸術活動  

    researchmap

受賞

  • Zoological Science Award

    2020年9月   日本動物学会  

     詳細を見る

  • 山陽新聞賞(学術功労)

    2016年  

     詳細を見る

    受賞国:日本国

    researchmap

  • 日本比較生理生化学会賞

    2014年  

     詳細を見る

    受賞国:日本国

    researchmap

  • 日本動物学会賞

    2013年  

     詳細を見る

    受賞国:日本国

    researchmap

  • Zoological Science Award

    2003年  

     詳細を見る

    受賞国:日本国

    researchmap

  • 日本動物学会藤井賞

    2003年  

     詳細を見る

    受賞国:日本国

    researchmap

  • 日本比較生理生化学会 吉田奨励賞

    1994年  

     詳細を見る

    受賞国:日本国

    researchmap

▼全件表示

 

担当授業科目

  • 分子遺伝学II (2021年度) 3・4学期  - [第3学期]水1,水2, [第4学期]水3,水4

  • 生体制御学III (2021年度) 1・2学期  - 木1,木2

  • 生体制御学IIIA (2021年度) 第1学期  - 木1,木2

  • 生体制御学IIIB (2021年度) 第2学期  - 木1,木2

  • 神経生物学I (2021年度) 3・4学期  - [第3学期]水3,水4, [第4学期]水1,水2

  • 神経生物学IB (2021年度) 第4学期  - 水1,水2

  • 時間生物学特論 (2020年度) 後期  - その他

  • 環境および時間生物学演習 (2020年度) 前期

  • 環境および時間生物学演習 (2020年度) 通年  - その他

  • 生体制御学III (2020年度) 1・2学期  - 木1,木2

  • 生体制御学IIIA (2020年度) 第1学期  - 木1,木2

  • 生体制御学IIIB (2020年度) 第2学期  - 木1,木2

  • 生物測時機構学 (2020年度) 前期  - 月1,月2

  • 神経生物学I (2020年度) 3・4学期  - [第3学期]水3,水4, [第4学期]水1,水2

  • 神経生物学IB (2020年度) 第4学期  - 水1,水2

▼全件表示