2022/01/12 更新

写真a

タニ アキヲ
谷 明生
TANI Akio
所属
資源植物科学研究所 准教授
職名
准教授
外部リンク

学位

  • 博士(農学) ( 京都大学 )

研究キーワード

  • Applied Microbiology

  • 応用微生物学

  • メタノール資化性細菌

  • ランタノイド依存微生物代謝

研究分野

  • ライフサイエンス / 応用微生物学

学歴

  • 京都大学   Graduate School, Division of Agriculture  

    - 2001年

      詳細を見る

  • 京都大学    

    - 2001年

      詳細を見る

    国名: 日本国

    researchmap

  • 京都大学   Faculty of Agriculture  

    - 1997年

      詳細を見る

    国名: 日本国

    researchmap

  • 京都大学   Faculty of Agriculture  

    - 1997年

      詳細を見る

経歴

  • - Associate Professor,Institute of Plant Science and Resources,Okayama University

    2014年

      詳細を見る

  • - 岡山大学資源植物科学研究所 准教授

    2014年

      詳細を見る

  • スイス連邦工科大学チューリッヒ校 研究員

    2012年 - 2013年

      詳細を見る

  • Researcher,ETH Zurich

    2012年 - 2013年

      詳細を見る

  • 岡山大学   Institute of Plant Science and Resources

    2004年 - 2014年

      詳細を見る

  • Assistant Professor,Institute of Plant Science and Resources,Okayama University

    2004年 - 2014年

      詳細を見る

▼全件表示

所属学協会

委員歴

  • - The society for Biotechnology, Japan  

    2011年   

      詳細を見る

  • - 日本生物工学会 中四国支部 幹事(2011-)  

    2011年   

      詳細を見る

 

論文

  • Discovery of lanthanide-dependent methylotrophy and screening methods for lanthanide-dependent methylotrophs. 国際誌

    Akio Tani, Ryoji Mitsui, Tomoyuki Nakagawa

    Methods in enzymology   650   1 - 18   2021年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER ACADEMIC PRESS INC  

    The lanthanide elements (Lns) affect the physiology and growth of certain microorganisms known as "Ln-responsive microorganisms." Among them, in 2011, it was first reported that strains of Methylobacterium exhibited high methanol dehydrogenase (MDH) activity when grown in the presence of Lns; the purified Ln-inducible MDH was identified as XoxF-type MDH, whose catalytic function had previously been unknown. XoxF was the first enzyme to be identified as Ln-dependent, and its function in methylotrophy is more fundamental and important than that of the corresponding Ca2+-dependent MDH MxaFI. XoxF is encoded in the genomes of methylotrophic as well as non-methylotrophic bacteria. Thus, Lns are among the most fascinating and important growth factors for bacteria that potentially utilize methanol. Bacteria that require Lns for methanol growth are called "Ln-dependent methylotrophs." Recent findings indicate that these microorganisms comprise an "Ln-dependent ecosystem" that we have not been able to reconstruct under laboratory conditions without Lns. In this chapter, we summarize methods for (1) screening of Ln-responsive microorganisms, (2) purification of native XoxFs from Ln-dependent methylotrophs, and (3) screening of Ln-dependent methylotrophs from natural environments, while providing a history of the discovery of the Ln-dependent methylotrophs.

    DOI: 10.1016/bs.mie.2021.01.031

    Web of Science

    PubMed

    researchmap

  • Comprehensive Comparative Genomics and Phenotyping of Methylobacterium Species. 国際誌

    Ola Alessa, Yoshitoshi Ogura, Yoshiko Fujitani, Hideto Takami, Tetsuya Hayashi, Nurettin Sahin, Akio Tani

    Frontiers in microbiology   12   740610 - 740610   2021年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:FRONTIERS MEDIA SA  

    The pink-pigmented facultative methylotrophs (PPFMs), a major bacterial group found in the plant phyllosphere, comprise two genera: Methylobacterium and Methylorubrum. They have been separated into three major clades: A, B (Methylorubrum), and C. Within these genera, however, some species lack either pigmentation or methylotrophy, which raises the question of what actually defines the PPFMs. The present study employed a comprehensive comparative genomics approach to reveal the phylogenetic relationship among the PPFMs and to explain the genotypic differences that confer their different phenotypes. We newly sequenced the genomes of 29 relevant-type strains to complete a dataset for almost all validly published species in the genera. Through comparative analysis, we revealed that methylotrophy, nitrate utilization, and anoxygenic photosynthesis are hallmarks differentiating the PPFMs from the other Methylobacteriaceae. The Methylobacterium species in clade A, including the type species Methylobacterium organophilum, were phylogenetically classified into six subclades, each possessing relatively high genomic homology and shared phenotypic characteristics. One of these subclades is phylogenetically close to Methylorubrum species; this finding led us to reunite the two genera into a single genus Methylobacterium. Clade C, meanwhile, is composed of phylogenetically distinct species that share relatively higher percent G+C content and larger genome sizes, including larger numbers of secondary metabolite clusters. Most species of clade C and some of clade A have the glutathione-dependent pathway for formaldehyde oxidation in addition to the H4MPT pathway. Some species cannot utilize methanol due to their lack of MxaF-type methanol dehydrogenase (MDH), but most harbor an XoxF-type MDH that enables growth on methanol in the presence of lanthanum. The genomes of PPFMs encode between two and seven (average 3.7) genes for pyrroloquinoline quinone-dependent alcohol dehydrogenases, and their phylogeny is distinctly correlated with their genomic phylogeny. All PPFMs were capable of synthesizing auxin and did not induce any immune response in rice cells. Other phenotypes including sugar utilization, antibiotic resistance, and antifungal activity correlated with their phylogenetic relationship. This study provides the first inclusive genotypic insight into the phylogeny and phenotypes of PPFMs.

    DOI: 10.3389/fmicb.2021.740610

    Web of Science

    PubMed

    researchmap

  • Methanol bioeconomy: promotion of rice crop yield in paddy fields with microbial cells prepared from natural gas-derived C1 compound. 国際誌

    Hiroya Yurimoto, Hiroyuki Iguchi, Do Thi Di Thien, Akio Tani, Yutaka Okumoto, Atsushi Ota, Takahiro Yamauchi, Takahiro Akashi, Yasuyoshi Sakai

    Microbial biotechnology   14 ( 4 )   1385 - 1396   2020年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    Methylotrophs, which can utilize methanol as a sole carbon source, are promising microorganisms to be exploited in a methanol-based bioeconomy, in which a variety of useful compounds are biotechnologically produced from natural gas-derived methanol. Pink-pigmented facultative methylotrophs (PPFMs) are common plant phyllospheric bacteria and are known to enhance seedling growth and total biomass of various plants. However, improvement of crop yield by inoculation of PPFMs at the field level has not been well investigated. We herein describe improvement of crop yield of several rice cultivars by foliar spraying of PPFMs. After selection of PPFM strains and rice cultivars by the in vitro seedling growth test, we further conducted paddy field experiments. The crop yield of the sake-brewing rice Oryza sativa cultivar Hakutsurunishiki was reproducibly improved in a commercial paddy field for over a 5-year period. A one-time foliar spray of PPFM cells (living or killed) or a cell wall polysaccharide fraction, after the heading date, acted in the phyllosphere and effectively improved crop yield. Our results show that the established process with PPFMs is feasible for improvement of food production in the methanol bioeconomy.

    DOI: 10.1111/1751-7915.13725

    Web of Science

    PubMed

    researchmap

  • Plant growth-promoting bacteria as potential bio-inoculants and biocontrol agents to promote black pepper plant cultivation. 国際誌

    Ee Tiing Lau, Akio Tani, Choy Yuen Khew, Yee Qin Chua, Siaw San Hwang

    Microbiological research   240   126549 - 126549   2020年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER GMBH  

    Black pepper production in Malaysia was restricted by various diseases. Hazardous chemical products appear to be the best solution to control diseases in black pepper cultivation. However, persistence of chemical residues in peppercorns could affect the quality of exports and consumptions. Application of fertilizers is crucial to sustain pepper growth and high yield. But, continuous use of chemical fertilizers could affect the soil ecosystem and eventually restrict nutrient uptake by pepper roots. Therefore, we propose biological approaches as an alternative solution instead of chemical products to sustain pepper cultivation in Malaysia. In this study, we have isolated a total of seven indigenous rhizobacteria antagonistic to soil-borne Fusarium solani, the causal fungus of slow decline, the most serious debilitating disease of black pepper in Malaysia. The isolated bacteria were identified as Bacillus subtilis, Bacillus siamensis, Brevibacillus gelatini, Pseudomonas geniculata, Pseudomonas beteli, Burkholderia ubonensis and Burkholderia territorii. These bacteria were effective in production of antifungal siderophore with the amount of 53.4 %-73.5 % per 0.5 mL of cell-free supernatants. The bacteria also produced appreciable amount of chitinase with chitinolytic index was ranged from 1.19 to 1.76. The bacteria have shown phosphate solubilizing index within 1.61 to 2.01. They were also efficient in ACC deaminase (0.52 mM-0.62 mM) and ammonia (60.3 mM-75.3 mM) production. The isolated antagonists were efficacious in stimulation of black pepper plant growth and root development through IAA (10.5 μg/mL-42.6 μg/mL) secretion. In conclusion, the isolated rhizobacteria are potent to be developed not only as biocontrol agents to minimize the utilization of hazardous chemicals in black pepper disease management, but also developed as bio-fertilizers to improve black pepper plant growth due to their capabilities in plant growth-promotion.

    DOI: 10.1016/j.micres.2020.126549

    Web of Science

    PubMed

    researchmap

  • Regulation of lanthanide-dependent methanol oxidation pathway in the legume symbiotic nitrogen-fixing bacterium Bradyrhizobium sp. strain Ce-3.

    Viagian Pastawan, Soya Suganuma, Kosuke Mizuno, Lun Wang, Akio Tani, Ryoji Mitsui, Kohei Nakamura, Masaya Shimada, Takashi Hayakawa, Nanung Agus Fitriyanto, Tomoyuki Nakagawa

    Journal of bioscience and bioengineering   130 ( 6 )   582 - 587   2020年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC BIOSCIENCE BIOENGINEERING JAPAN  

    Lanthanide (Ln)-dependent XoxF-type methanol dehydrogenase (MDH) genes can be found in bacteria that are not believed to be methylotrophs, and studies on their methylotrophic pathways and their use of Ln are now emerging. Ln-dependent methanol utilization in Bradyrhizobium sp. strain Ce-3, which belongs to the Bradyrhizobium elkanii superclade (clade II), was investigated in this study. Strain Ce-3 was able to grow in a media containing methanol as a sole carbon source and light Ln (L-Ln, i.e., La3+, Ce3+, Pr3+, and Nd3+), whereas the strain did not show any growth with Ca2+ or the heavy Ln, Sm3+. We found that the uptake of L-Ln is enhanced mainly by methanol and L-Ln species, and the strain incorporates each L-Ln species evenly into the cell. The genome of strain Ce-3 encodes the xox cluster for Ln-dependent methanol dehydrogenase (xoxF) and the enzymes participating in the methanol oxidation pathway (xoxG, fldA, and gfaA) and regulation (xoxR), but the gene encoding formate dehydrogenase (FDH) was not found in the cluster. MDH, formaldehyde dehydrogenase, and FDH activities were induced by methanol/Ln. Moreover, expression of the genes on the xox cluster was upregulated by methanol/Ln. Based on these results, we concluded that strain Ce-3 possesses a complete L-Ln-dependent methanol oxidation pathway, which is dissimilar to plant phyllospheric bacteria, Methylobacterium species, with a transport system for L-Ln species.

    DOI: 10.1016/j.jbiosc.2020.07.012

    Web of Science

    PubMed

    researchmap

  • The Presence of the Hairy-Root-Disease-Inducing (Ri) Plasmid in Wheat Endophytic Rhizobia Explains a Pathogen Reservoir Function of Healthy Resistant Plants. 国際誌

    Byoungwoo Kang, Taichi Maeshige, Aya Okamoto, Yui Kataoka, Shinji Yamamoto, Kazuhide Rikiishi, Akio Tani, Hiroyuki Sawada, Katsunori Suzuki

    Applied and environmental microbiology   86 ( 17 )   2020年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    A large number of strains in the Rhizobium radiobacter species complex (biovar 1 Agrobacterium) have been known as causative pathogens for crown gall and hairy root diseases. Strains within this complex were also found as endophytes in many plant species with no symptoms. The aim of this study was to reveal the endophyte variation of this complex and how these endophytic strains differ from pathogenic strains. In this study, we devised a simple but effective screening method by exploiting the high resolution power of mass spectrometry. We screened endophyte isolates from young wheat and barley plants, which are resistant to the diseases, and identified seven isolates from wheat as members of the R. radiobacter species complex. Through further analyses, we assigned five strains to the genomovar (genomic group) G1 and two strains to G7 in R. radiobacter Notably, these two genomovar groups harbor many known pathogenic strains. In fact, the two G7 endophyte strains showed pathogenicity on tobacco, as well as the virulence prerequisites, including a 200-kbp Ri plasmid. All five G1 strains possessed a 500-kbp plasmid, which is present in well-known crown gall pathogens. These data strongly suggest that healthy wheat plants are reservoirs for pathogenic strains of R. radiobacter IMPORTANCE Crown gall and hairy root diseases exhibit very wide host-plant ranges that cover gymnosperm and dicot plants. The Rhizobium radiobacter species complex harbors causative agents of the two diseases. Recently, endophyte isolates from many plant species have been assigned to this species complex. We isolated seven endophyte strains belonging to the species complex from wheat plants and revealed their genomovar affiliations and plasmid profile. The significance of this study is the finding of the genomovar correlation between the endophytes and the known pathogens, the presence of a virulence ability in two of the seven endophyte strains, and the high ratio of the pathogenic strains in the endophyte strains. This study therefore provides convincing evidence that could unravel the mechanism that maintains pathogenic agents of this species and sporadically delivers them to susceptible plants.

    DOI: 10.1128/AEM.00671-20

    Web of Science

    PubMed

    researchmap

  • Lanthanide-Dependent Methanol and Formaldehyde Oxidation in Methylobacterium aquaticum Strain 22A 国際誌

    Patcha Yanpirat, Yukari Nakatsuji, Shota Hiraga, Yoshiko Fujitani, Terumi Izumi, Sachiko Masuda, Ryoji Mitsui, Tomoyuki Nakagawa, Akio Tani

    Microorganisms   8 ( 6 )   822 - 822   2020年5月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MDPI AG  

    Lanthanides (Ln) are an essential cofactor for XoxF-type methanol dehydrogenases (MDHs) in Gram-negative methylotrophs. The Ln3+ dependency of XoxF has expanded knowledge and raised new questions in methylotrophy, including the differences in characteristics of XoxF-type MDHs, their regulation, and the methylotrophic metabolism including formaldehyde oxidation. In this study, we genetically identified one set of Ln3+- and Ca2+-dependent MDHs (XoxF1 and MxaFI), that are involved in methylotrophy, and an ExaF-type Ln3+-dependent ethanol dehydrogenase, among six MDH-like genes in Methylobacterium aquaticum strain 22A. We also identified the causative mutations in MxbD, a sensor kinase necessary for mxaF expression and xoxF1 repression, for suppressive phenotypes in xoxF1 mutants defective in methanol growth even in the absence of Ln3+. Furthermore, we examined the phenotypes of a series of formaldehyde oxidation-pathway mutants (fae1, fae2, mch in the tetrahydromethanopterin (H4MPT) pathway and hgd in the glutathione-dependent formaldehyde dehydrogenase (GSH) pathway). We found that MxaF produces formaldehyde to a toxic level in the absence of the formaldehyde oxidation pathways and that either XoxF1 or ExaF can oxidize formaldehyde to alleviate formaldehyde toxicity in vivo. Furthermore, the GSH pathway has a supportive role for the net formaldehyde oxidation in addition to the H4MPT pathway that has primary importance. Studies on methylotrophy in Methylobacterium species have a long history, and this study provides further insights into genetic and physiological diversity and the differences in methylotrophy within the plant-colonizing methylotrophs.

    DOI: 10.3390/microorganisms8060822

    PubMed

    researchmap

  • Lanthanide-Dependent Methanol and Formaldehyde Oxidation in Methylobacterium aquaticum Strain 22A. 国際誌

    Patcha Yanpirat, Yukari Nakatsuji, Shota Hiraga, Yoshiko Fujitani, Terumi Izumi, Sachiko Masuda, Ryoji Mitsui, Tomoyuki Nakagawa, Akio Tani

    Microorganisms   8 ( 6 )   2020年5月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MDPI  

    Lanthanides (Ln) are an essential cofactor for XoxF-type methanol dehydrogenases (MDHs) in Gram-negative methylotrophs. The Ln3+ dependency of XoxF has expanded knowledge and raised new questions in methylotrophy, including the differences in characteristics of XoxF-type MDHs, their regulation, and the methylotrophic metabolism including formaldehyde oxidation. In this study, we genetically identified one set of Ln3+- and Ca2+-dependent MDHs (XoxF1 and MxaFI), that are involved in methylotrophy, and an ExaF-type Ln3+-dependent ethanol dehydrogenase, among six MDH-like genes in Methylobacterium aquaticum strain 22A. We also identified the causative mutations in MxbD, a sensor kinase necessary for mxaF expression and xoxF1 repression, for suppressive phenotypes in xoxF1 mutants defective in methanol growth even in the absence of Ln3+. Furthermore, we examined the phenotypes of a series of formaldehyde oxidation-pathway mutants (fae1, fae2, mch in the tetrahydromethanopterin (H4MPT) pathway and hgd in the glutathione-dependent formaldehyde dehydrogenase (GSH) pathway). We found that MxaF produces formaldehyde to a toxic level in the absence of the formaldehyde oxidation pathways and that either XoxF1 or ExaF can oxidize formaldehyde to alleviate formaldehyde toxicity in vivo. Furthermore, the GSH pathway has a supportive role for the net formaldehyde oxidation in addition to the H4MPT pathway that has primary importance. Studies on methylotrophy in Methylobacterium species have a long history, and this study provides further insights into genetic and physiological diversity and the differences in methylotrophy within the plant-colonizing methylotrophs.

    DOI: 10.3390/microorganisms8060822

    Web of Science

    PubMed

    researchmap

  • Preference for particular lanthanide species and thermal stability of XoxFs in Methylorubrum extorquens strain AM1 査読 国際誌

    Lun Wang, Ayumi Hibino, Souya Suganuma, Akio Ebihara, Satoshi Iwamoto, Ryoji Mitsui, Akio Tani, Masaya Shimada, Takashi Hayakawa, Tomoyuki Nakagawa

    Enzyme and Microbial Technology   136   109518 - 109518   2020年5月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

    © 2020 Elsevier Inc. XoxF-type methanol dehydrogenase was recently found to be lanthanide-dependent, while its counterpart MxaF is Ca2+-dependent. The lanthanide (Ln) series consists of 15 different elements, all of which exist in nature, although at different relative abundances. XoxF from Methylorubrum extorquens strain AM1 has been shown to be induced by four light Ln species (La3+ to Nd3+). The preference of XoxFs for certain co-existing Ln species and the catalytic activity and stability of XoxF metallated with different Ln species have not been well investigated. In this study, we found that (i) strain AM1 cells preferentially utilize La3+ rather than Nd3+ for growth, (ii) XoxF purified from cells grown with a La3+ and Nd3+ mixture contained a larger proportion of La3+, and (iii) La3+-metallated XoxF has higher activity and thermal stability than Nd3+-metallated XoxF, although (iv) both enzymes showed unchanged surface charges. Thermal shift assay in particular revealed that metallation affects the temperature for subunit denaturation but not for subunit dissociation. We concluded that, although La3+ and Nd3+ have similar distributions in nature, XoxF could chose La3+ preferentially, likely because of its higher Lewis acidity, which is important for the catalytic activity of the enzyme.

    DOI: 10.1016/j.enzmictec.2020.109518

    Web of Science

    Scopus

    PubMed

    researchmap

  • Methylotenera oryzisoli sp. nov., a lanthanide-dependent methylotrophic bacteria isolated from rice field soil. 国際誌

    Haoxin Lv, Nurettin Sahin, Akio Tani

    International journal of systematic and evolutionary microbiology   70 ( 4 )   2713 - 2718   2020年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MICROBIOLOGY SOC  

    A new lanthanide (Ln3+)-dependent methanol-utilizing bacterial strain, La3113T, was isolated from rice field soil and its taxonomic position was investigated using polyphasic approaches. The strain was aerobic, Gram-stain-negative, strongly motile, catalase-positive and cytochrome oxidase-positive. It could neither catalyse the hydrolysis of urea nor reduce nitrate to nitrite. Growth was observed within a temperature range of 10-40 °C and a pH range of 6-8, with optimum growth at 28 °C and pH 7. Methylamine was utilized as the single source of energy, carbon and nitrogen, and it was oxidized by methylamine dehydrogenase. C16 : 1  ω7c, C16 : 1  ω6c and C16 : 0 were the dominant cellular fatty acids. Its draft genome (2.67 Mbp and 44.9 mol% G+C content) encodes genes including three Ln3+-dependent methanol dehydrogenase (XoxF-type MDH) genes, those for formaldehyde assimilation (ribulose monophosphate pathway), formate dehydrogenases and methylamine dehydrogenases, but not Ca2+-dependent MDH (MxaFI-MDH), which characterizes the species as a Ln3+-dependent methylotroph. The 16S rRNA gene sequence showed that strain La3113T belongs to the genus Methylotenera and is closely related to Methylotenera mobilis JLW8T (98.29 % identity). The digital DNA-DNA hybridization (dDDH) values (less than 30 %) and average nucleotide identity (ANI) values (less than 85 %) between genomes of strain La3113T and related type strains were lower than the thresholds for species delineation (70 % for dDDH and 95-96 % for ANI). On the basis of these polyphasic approaches, we propose a novel Methylotenera species, Methylotenera oryzisoli sp. nov. (type strain La3113T=NBRC 111954T=DSM 103219T).

    DOI: 10.1099/ijsem.0.004098

    Web of Science

    PubMed

    researchmap

  • Isolation and Characterization of Genes Responsible for Naphthalene Degradation from Thermophilic Naphthalene Degrader, Geobacillus sp. JF8. 査読 国際誌

    Daisuke Miyazawa, Le Thi Ha Thanh, Akio Tani, Masaki Shintani, Nguyen Hoang Loc, Takashi Hatta, Kazuhide Kimbara

    Microorganisms   8 ( 1 )   2019年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MDPI  

    Geobacillus sp. JF8 is a thermophilic biphenyl and naphthalene degrader. To identify the naphthalene degradation genes, cis-naphthalene dihydrodiol dehydrogenase was purified from naphthalene-grown cells, and its N-terminal amino acid sequence was determined. Using a DNA probe encoding the N-terminal region of the dehydrogenase, a 10-kb DNA fragment was isolated. Upstream of nahB, a gene for dehydrogenase, there were two open reading frames which were designated as nahAc and nahAd, respectively. The products of nahAc and nahAd were predicted to be alpha and beta subunit of ring-hydroxylating dioxygenases, respectively. Phylogenetic analysis of amino acid sequences of NahB indicated that it did not belong to the cis-dihydrodiol dehydrogenase group that includes those of classical naphthalene degradation pathways. Downstream of nahB, four open reading frames were found, and their products were predicted as meta-cleavage product hydrolase, monooxygenase, dehydrogenase, and gentisate 1,2-dioxygenase, respectively. A reverse transcriptase-PCR analysis showed that transcription of nahAcAd was induced by naphthalene. These findings indicate that we successfully identified genes involved in the upper pathway of naphthalene degradation from a thermophilic bacterium.

    DOI: 10.3390/microorganisms8010044

    Web of Science

    PubMed

    researchmap

  • Lanthanide-dependent methanol dehydrogenase from the legume symbiotic nitrogen-fixing bacterium Bradyrhizobium diazoefficiens strain USDA110 査読 国際誌

    Lun Wang, Soya Suganuma, Ayumi Hibino, Ryoji Mitsui, Akio Tani, Takashi Matsumoto, Akio Ebihara, Nanung Agus Fitriyanto, Ambar Pertiwiningrum, Masaya Shimada, Takashi Hayakawa, Tomoyuki Nakagawa

    Enzyme and Microbial Technology   130   109371 - 109371   2019年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    © 2019 Elsevier Inc. The legume symbiotic nitrogen-fixing bacterium, B. diazoefficiens strain USDA110, utilizes methanol for growth in the presence of light lanthanides, such as La3+, Ce3+, Pr3+ or Nd3+, and its cells possess significant methanol dehydrogenase (MDH) activity. We purified MDH to homogeneity from B. diazoefficiens strain USDA110 grown in a methanol/Ce3+ medium; the protein was identified as XoxF5-type MDH (blr6213). The purified XoxF contained 0.58 cerium atoms per enzyme subunit. Moreover, the in-solution structure of XoxF was analyzed by small angle X-ray scattering (SAXS) analysis; the radius of gyration (Rg) and maximum particle dimension (Dmax) of XoxF were calculated to be 32.3 and 96.8 Å, respectively, suggesting that XoxF adopts a dimer structure in solution. These results show that B. diazoefficiens strain USDA110 has XoxF, a lanthanides-dependent MDH, which has methanol oxidation activity and is induced by methanol/lanthanaides, and that lanthanide is one of the important factors in methanol utilization by the strain.

    DOI: 10.1016/j.enzmictec.2019.109371

    Web of Science

    Scopus

    PubMed

    researchmap

  • Bacteria with natural chemotaxis towards methanol revealed by chemotaxis fishing technique. 国際誌

    Yosef Hamba Tola, Yoshiko Fujitani, Akio Tani

    Bioscience, biotechnology, and biochemistry   83 ( 11 )   2163 - 2171   2019年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Motile bacteria often exhibit chemotaxis toward favorable compounds. However, the diversity of bacteria that are attracted to a given substance is largely unknown. This study aimed to reveal the diversity of bacteria with natural chemotaxis towards methanol. We tried to enrich environmental chemotactic bacteria using a glass capillary that is half-filled with methanol solidified with agarose as a trap ("chemotaxis fishing"). The pilot experiment using methanol-chemotactic Methylobacterium aquaticum strain 22A enriched the cells by 46-fold. The method was then applied to bacterial suspensions from paddy water and plants. Depending on the isolation sources and the methods of motility induction, methylotrophic bacteria were enriched 1.2-330-fold. The fished isolates belong to 32 species in 18 genera, mainly containing Acinetobacter, Methylobacterium and Pseudomonas species. Our chemotaxis fishing unveiled a part of diversity of the bacteria with natural chemotaxis towards methanol.

    DOI: 10.1080/09168451.2019.1637715

    Web of Science

    PubMed

    researchmap

  • Brown planthopper honeydew-associated symbiotic microbes elicit momilactones in rice 査読 国際誌

    Wari David, Alamgir Kabir Md, Mujiono Kadis, Hojo Yuko, Tani Akio, Shinya Tomonori, Nakatani Hiroko, Galis Ivan

    PLANT SIGNALING & BEHAVIOR   14 ( 11 )   1655335 - 1655335   2019年8月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1080/15592324.2019.1655335

    Web of Science

    PubMed

    researchmap

  • Honeydew-associated microbes elicit defense responses against brown planthopper in rice. 査読 国際誌

    David Wari, Md Alamgir Kabir, Kadis Mujiono, Yuko Hojo, Tomonori Shinya, Akio Tani, Hiroko Nakatani, Ivan Galis

    Journal of experimental botany   70 ( 5 )   1683 - 1696   2019年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Feeding of sucking insects, such as the rice brown planthopper (Nilaparvata lugens; BPH), causes only limited mechanical damage on plants that is otherwise essential for injury-triggered defense responses against herbivores. In pursuit of complementary BPH elicitors perceived by plants, we examined the potential effects of BPH honeydew secretions on the BPH monocot host, rice (Oryza sativa). We found that BPH honeydew strongly elicits direct and putative indirect defenses in rice, namely accumulation of phytoalexins in the leaves, and release of volatile organic compounds from the leaves that serve to attract natural enemies of herbivores, respectively. We then examined the elicitor active components in the honeydew and found that bacteria in the secretions are responsible for the activation of plant defense. Corroborating the importance of honeydew-associated microbiota for induced plant resistance, BPHs partially devoid of their microbiota via prolonged antibiotics ingestion induced significantly less defense in rice relative to antibiotic-free insects applied to similar groups of plants. Our data suggest that rice plants may additionally perceive herbivores via their honeydew-associated microbes, allowing them to discriminate between incompatible herbivores-that do not produce honeydew-and those that are compatible and therefore dangerous.

    DOI: 10.1093/jxb/erz041

    Web of Science

    Scopus

    PubMed

    researchmap

    その他リンク: http://orcid.org/0000-0001-9840-8845

  • Distribution of 1-aminocyclopropane-1-carboxylate deaminase and d-cysteine desulfhydrase genes among type species of the genus Methylobacterium 査読 国際誌

    Galina A. Ekimova, Dmitry N. Fedorov, Akio Tani, Nina V. Doronina, Yuri A. Trotsenko

    Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology   111 ( 10 )   1 - 12   2018年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Netherlands  

    The presence of 1-aminocyclopropane-1-carboxylate (ACC) deaminase determines the ability of bacteria to increase the resistance of plants to various types of stress. The genes of ACC deaminase (acdS) and the closely related enzyme d-cysteine desulfhydrase (dcyD) were searched in type strains of various representatives of the genus Methylobacterium. Using PCR screening and in silico searching in the available complete genome sequences of type strains, the genes were found in 28 of 48 species of the genus. Phylogenetic analysis of amino acid sequences of proteins revealed two large groups of sequences of the AcdS protein and one of the DcyD protein. The distribution of these groups correlates well with the phylogenetic tree based on the sequences of the 16S rRNA genes, which apparently indicates a different evolutionary adaptation to association with plants in the representatives of these groups. For the first time for aerobic methylotrophs it was demonstrated that the gene dcyD encodes d-cysteine desulfhydrase by cloning and recombinant protein characterization.

    DOI: 10.1007/s10482-018-1061-5

    Web of Science

    Scopus

    PubMed

    researchmap

  • Isolation and genomic characterization of Novimethylophilus kurashikiensis gen. nov. sp. nov., a new lanthanide-dependent methylotrophic species of Methylophilaceae 査読 国際誌

    Haoxin Lv, Nurettin Sahin, Akio Tani

    Environmental Microbiology   20 ( 3 )   1204 - 1223   2018年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Blackwell Publishing Ltd  

    Recently, it has been found that two types of methanol dehydrogenases (MDHs) exist in Gram-negative bacterial methylotrophs, calcium-dependent MxaFI-MDH and lanthanide-dependent XoxF-MDH and the latter is more widespread in bacterial genomes. We aimed to isolate and characterize lanthanide-dependent methylotrophs. The growth of strain La2-4T on methanol, which was isolated from rice rhizosphere soil, was strictly lanthanide dependent. Its 16S rRNA gene sequence showed only 93.4% identity to that of Methylophilus luteus MimT, and the name Novimethylophilus kurashikiensis gen. nov. sp. nov. is proposed. Its draft genome (ca. 3.69 Mbp, G + C content 56.1 mol%) encodes 3579 putative CDSs and 84 tRNAs. The genome harbors five xoxFs but no mxaFI. XoxF4 was the major MDH in the cells grown on methanol and methylamine, evidenced by protein identification and quantitative PCR analysis. Methylamine dehydrogenase gene was absent in the La2-4T genome, while genes for the glutamate-mediated methylamine utilization pathway were detected. The genome also harbors those for the tetrahydromethanopterin and ribulose monophosphate pathways. Additionally, as known species, isolates of Burkholderia ambifaria, Cupriavidus necator and Dyadobacter endophyticus exhibited lanthanide dependent growth on methanol. Thus, lanthanide can be used as an essential growth factor for methylotrophic bacteria that do not harbor MxaFI-MDH.

    DOI: 10.1111/1462-2920.14062

    Web of Science

    Scopus

    PubMed

    researchmap

  • Lanthanide-dependent regulation of methylotrophy in Methylobacterium aquaticum strain 22A 査読

    Sachiko Masuda, Yutaka Suzuki, Yoshiko Fujitani, Ryoji Mitsui, Tomoyuki Nakagawa, Masaki Shintani, Akio Tani

    mSphere   3 ( 1 )   2018年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    Methylobacterium species are representative of methylotrophic bacteria. Their genomes usually encode two types of methanol dehydrogenases (MDHs): MxaF and XoxF. The former is a Ca2+-dependent enzyme, and the latter was recently determined to be a lanthanide-dependent enzyme that is necessary for the expression of mxaF. This finding revealed the unexpected and important roles of lanthanides in bacterial methylotrophy. In this study, we performed transcriptome sequencing (RNA-seq) analysis using M. aquaticum strain 22A grown in the presence of different lanthanides. Expression of mxaF and xoxF1 genes showed a clear inverse correlation in response to La3+. We observed downregulation of formaldehyde oxidation pathways, high formaldehyde dehydrogenase activity, and low accumulation of formaldehyde in the reaction with cells grown in the presence of La33+
    this might be due to the direct oxidation of methanol to formate by XoxF1. Lanthanides induced the transcription of AT-rich genes, the function of most of which was unknown, and genes possibly related to cellular survival, as well as other MDH homologues. These results revealed not only the metabolic response toward altered primary methanol oxidation, but also the possible targets to be investigated further in order to better understand methylotrophy in the presence of lanthanides.

    DOI: 10.1128/mSphere.00462-17

    Web of Science

    Scopus

    PubMed

    researchmap

  • Ergothioneine production using Methylobacterium species, yeast, and fungi 査読

    Yoshiko Fujitani, Kabir Md Alamgir, Akio Tani

    Journal of Bioscience and Bioengineering   126 ( 6 )   715 - 722   2018年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier B.V.  

    Ergothioneine (EGT) is a sulfur-containing, anti-oxidative amino acid derived from histidine. EGT is synthesized in bacteria and fungi but not in animals and plants, and is now recognized as important for human health. Its cost-effective fermentative production has not been elucidated due to the lack of information for productive microorganisms. In this study, we doubled the gene copy for EGT synthesis and deleted the histidine ammonia-lyase gene in a potent EGT-producing methylotrophic bacterium Methylobacterium aquaticum strain 22A, and optimized its culture conditions, resulting in increased EGT production of 7.0 mg EGT/g dry cell weight and 100 μg EGT/5 mL/7 days. In addition, through screening we found EGT-producing eukaryotic strains of Aureobasidium pullulans and Rhodotorula mucilaginosa, which can produce 1.0 and 3.2 mg EGT/g dry cell weight, 70 and 120 μg EGT/5 mL/7 days, respectively. This study proposes practical uses of potent EGT-producing recombinant Methylobacterium species and non-recombinant yeast and fungal strains.

    DOI: 10.1016/j.jbiosc.2018.05.021

    Web of Science

    Scopus

    PubMed

    researchmap

  • Genomic characterization of methylotrophy of Oharaeibacter diazotrophicus strain SM30<sup>T</sup>. 査読

    Lv H, Tani A

    Journal of bioscience and bioengineering   126 ( 6 )   667 - 675   2018年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.jbiosc.2018.05.023

    Web of Science

    Scopus

    PubMed

    researchmap

  • Oharaeibacter diazotrophicus gen. nov., sp nov., a diazotrophic and facultatively methylotrophic bacterium, isolated from rice rhizosphere 査読 国際誌

    Haoxin Lv, Sachiko Masuda, Yoshiko Fujitani, Nurettin Sahin, Akio Tani

    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY   67 ( 3 )   576 - 582   2017年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MICROBIOLOGY SOC  

    A novel facultatively methanol-utilizing bacterial strain, SM30(T), was isolated from rice rhizosphere. Strain SM30(T) was Gram-stain-negative, aerobic, motile, short rods, and grew optimally at pH 7 and at 28 degrees C. It could tolerate 0 to 2%(w/v) NaCl. Based on 16S rRNA gene sequence comparisons, strain SM30(T) was most closely related to Pleomorphomonas oryzae DSM 16300(T), with a low similarity of 94.17 %. One of the lanthanide metals, lanthanum, could enhance its growth slightly on methanol. Phylogenetic trees, based on the mxaF, xoxF and cpn60 genes of SM30(T) showed its distinct phylogenetic position with respect to species with validly published names. Polymerase chain reaction (PCR) amplification of the nifH and growth on nitrogen-free medium indicated that strain SM30(T) is a diazotroph. The major cellular fatty acids were summed feature 8 (containing 18:1 omega 7c and 18:1 omega 6c) and cyclo 19:0 omega 8c. The major quinone was ubiquinone 10. The DNA G+C content was 74.6 mol%. Based on the genotypic and phenotypic characteristics, strain SM30(T) represents a novel genus and species, for which the name Oharaeibacter diazotrophicus gen. nov., sp. nov. is proposed with the type strain SM30(T) (= NBRC 111955(T) = DSM 102969(T)).

    DOI: 10.1099/ijsem.0.001660

    Web of Science

    PubMed

    researchmap

  • A capsidless ssRNA virus hosted by an unrelated dsRNA virus 査読 国際誌

    Rui Zhang, Sakae Hisano, Akio Tani, Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

    NATURE MICROBIOLOGY   1 ( 1 )   15001 - 15001   2016年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Viruses typically encode the capsid that encases their genome, while satellite viruses do not encode a replicase and depend on a helper virus for their replication(1). Here, we report interplay between two RNA viruses, yado-nushi virus 1 (YnV1) and yado-kari virus 1 (YkV1), in a phytopathogenic fungus, Rosellinia necatrix(2). YkV1 has a close phylogenetic affinity to positive-sense, single-stranded (+)ssRNA viruses such as animal caliciviruses(3), while YnV1 has an undivided double-stranded (ds) RNA genome with a resemblance to fungal toti-viruses(4). Virion transfection and infectious full-length cDNA transformation has shown that YkV1 depends on YnV1 for viability, although it probably encodes functional RNA-dependent RNA polymerase (RdRp). Immunological and molecular analyses have revealed trans-encapsidation of not only YkV1 RNA but also RdRp by the capsid protein of the other virus (YnV1), and enhancement of YnV1 accumulation by YkV1. This study demonstrates interplay in which the capsidless (+) ssRNA virus (YkV1), hijacks the capsid protein of the dsRNA virus (YnV1), and replicates as if it were a dsRNA virus.

    DOI: 10.1038/NMICROBIOL.2015.1

    Web of Science

    PubMed

    researchmap

  • レアアースを必須因子として要求する新たな代謝系 植物共生細菌たちがもつレアアース依存型C<sub>1</sub>代謝

    中川智行, 三井亮司, 谷明生, 河合啓一

    化学と生物   53 ( 11 )   744 - 750   2015年10月

     詳細を見る

    記述言語:日本語   出版者・発行元:日本農芸化学会  

    DOI: 10.1271/kagakutoseibutsu.53.744

    J-GLOBAL

    researchmap

  • Production of ergothioneine by Methylobacterium species 査読 国際誌

    Kabir M. Alamgir, Sachiko Masuda, Yoshiko Fujitani, Fumio Fukuda, Akio Tani

    FRONTIERS IN MICROBIOLOGY   6   1185 - 1185   2015年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:FRONTIERS MEDIA SA  

    Metabolomic analysis revealed that Methylobacterium cells accumulate a large amount of ergothioneine (EGT), which is a sulfur containing, non-proteinogenic, antioxidative amino acid derived from histidine. EGT biosynthesis and its role in methylotrophy and physiology for plant surface-symbiotic Methylobacterium species were investigated in this study. Almost all Methylobacterium type strains can synthesize FGT. We selected one of the most productive strains (M. aquaticum strain 22A isolated from a moss), and investigated the feasibility of fermentative EGT production through optimization of the culture condition. Methanol as a carbon source served as the best substrate for production. The productivity reached up to 1000 mu g/100 ml culture (1200 mu g/g wet weight cells, 6.3 mg/g dry weight) in 38 days. Next, we identified the genes (egtBD) responsible for EGT synthesis, and generated a deletion mutant defective in EGT production. Compared to the wild type, the mutant showed better growth on methanol and on the plant surface as well as severe susceptibility to heat treatment and irradiation of ultraviolet (UV) and sunlight. These results suggested that EGT is not involved in methylotrophy, but is involved in their phyllospheric lifestyle fitness of the genus in natural conditions.

    DOI: 10.3389/fmicb.2015.01185

    Web of Science

    PubMed

    researchmap

  • Bacterial-biota dynamics of eight bryophyte species from different ecosystems 査読 国際誌

    Faisal Hammad Mekky Koua, Kazuhide Kimbara, Akio Tani

    SAUDI JOURNAL OF BIOLOGICAL SCIENCES   22 ( 2 )   204 - 210   2015年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Despite the importance of bryophyte-associated microorganisms in various ecological aspects including their crucial roles in the soil-enrichment of organic mass and N-2 fixation, nonetheless, little is known about the microbial diversity of the bryophyte phyllospheres (epi-/endophytes). To get insights into bacterial community structures and their dynamics on the bryophyte habitats in different ecosystems and their potential biological roles, we utilized the 16S rRNA gene PCR-DGGE and subsequent phylogenetic analyses to investigate the bacterial community of eight bryophyte species collected from three distinct ecosystems from western Japan. Forty-two bacterial species belonging to gamma-proteobacteria and Firmicutes with 71.4% and 28.6%, respectively, were identified among 90 DGGE gel band population. These DGGE-bands were assigned to 13 different genera with obvious predomination the genus Clostridium with 21.4% from the total bacterial community. These analyses provide new insights into bryophyte-associated bacteria and their relations to the ecosystems. (C) 2014 Production and hosting by Elsevier B.V. on behalf of King Saud University.

    DOI: 10.1016/j.sjbs.2014.07.009

    Web of Science

    PubMed

    researchmap

  • Complete Genome Sequence of Methylobacterium aquaticum Strain 22A, Isolated from Racomitrium japonicum Moss

    Akio Tani, Yoshitoshi Ogura, Tetsuya Hayashi, Kazuhide Kimbara

    MICROBIOLOGY RESOURCE ANNOUNCEMENTS   3 ( 2 )   2015年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    Methylobacterium species colonize plant surfaces and utilize methanol emitted from plants. Methylobacterium aquaticum strain 22A was isolated from a hydroponic culture of a moss, Racomitrium japonicum, and is a potent plant growth promoter. The complete genome sequencing of the strain confirmed the presence of genes related to plant growth promotion and methylotrophy.

    DOI: 10.1128/genomeA.00266-15

    Web of Science

    researchmap

  • Methylobacterium Species Promoting Rice and Barley Growth and Interaction Specificity Revealed with Whole-Cell Matrix-Assisted Laser Desorption/Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF/MS) Analysis. 査読 国際誌

    Akio Tani, Nurettin Sahin, Yoshiko Fujitani, Akiko Kato, Kazuhiro Sato, Kazuhide Kimbara

    PloS one   10 ( 6 )   e0129509   2015年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Methylobacterium species frequently inhabit plant surfaces and are able to utilize the methanol emitted from plants as carbon and energy sources. As some of the Methylobacterium species are known to promote plant growth, significant attention has been paid to the mechanism of growth promotion and the specificity of plant-microbe interactions. By screening our Methylobacterium isolate collection for the high growth promotion effect in vitro, we selected some candidates for field and pot growth tests for rice and barley, respectively. We found that inoculation resulted in better ripening of rice seeds, and increased the size of barley grains but not the total yield. In addition, using whole-cell matrix-assister laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF/MS) analysis, we identified and classified Methylobacterium isolates from Methylobacterium-inoculated rice plants. The inoculated species could not be recovered from the rice plants, and in some cases, the Methylobacterium community structure was affected by the inoculation, but not with predomination of the inoculated species. The isolates from non-inoculated barley of various cultivars grown in the same field fell into just two species. These results suggest that there is a strong selection pressure at the species level of Methylobacterium residing on a given plant species, and that selection of appropriate species that can persist on the plant is important to achieve growth promotion.

    DOI: 10.1371/journal.pone.0129509

    Web of Science

    PubMed

    researchmap

  • Proteome analysis of acrylamide-induced proteins in a novel acrylamide-degrader Enterobacter aerogenes by 2D electrophoresis and MALDI-TOF-MS 査読

    Jittima Charoenpanich, Akio Tani

    Chiang Mai University Journal of Natural Sciences   13 ( 1 )   11 - 22   2014年1月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Despite tremendous advances in understanding the microbial degradation of acrylamide, reports on the nature of the two-dimensional protein patterns for acrylamide-degrading bacteria are not yet available. This work, focusing on the acrylamide-inducible proteins, studied the response of Enterobacter aerogenes, a novel acrylamide-degrading bacterium, to acrylamide. Proteome analysis was applied using 2D-polyacrylamide gel electrophoresis and matrixassisted laser desorption/ionisation-time of flight mass spectrometry to identify proteins differentially expressed from E. aerogenes grown on acrylamide. Six protein homologues with amidohydrolase, urease accessory protein, quaternary ammonium compound resistance proteins, dipeptide transport protein, Omp36 osmoporin and large conductance mechanosensitive channel proteins (MscL) are seemingly involved in acrylamide stress response and its degradation. Five proteins identified as GroEL-like chaperonin, ArsR-transcriptional regulator, Ts- and Tu-elongation factor and trigger factor and four proteins (phosphoglycerate kinase, ATP synthase β-subunit, malate dehydrogenase and succinyl-CoA synthetase α-subunit) responsive for the adaption of E. aerogenes in the presence of acrylamide.

    DOI: 10.12982/cmujns.2014.0016

    Scopus

    researchmap

  • Dominant Colonization and Inheritance of Methylobacterium sp Strain OR01 on Perilla Plants 査読 国際誌

    Masayuki Mizuno, Hiroya Yurimoto, Hiroyuki Iguchi, Akio Tani, Yasuyoshi Sakai

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   77 ( 7 )   1533 - 1538   2013年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Pink-pigmented facultative methylotrophs (PPFMs) are major inhabitants of the phyllosphere. In a preceding study, we found that perilla plants harbor a dominant population of PPFMs on their leaves and seeds, and that the closest relative of PPFMs (Methylbacterium sp. strain OR01 as representative strain) isolated from red perilla seeds was M. fujisawaense DSM5686(T). In the present study, the specific interaction between red perilla and Methylobacterium species was investigated. All the PPFMs isolated from red perilla seeds harvested in the Ohara area of Kyoto, Japan in 2009, 2010, and 2011 and the PPFMs isolated from red perilla leaves planted at four geographically different places in Japan had 16S rRNA sequences identical to that of strain OR01. Direct transmission of PPFMs from seeds to leaves and the competitiveness of strain OR01 were confirmed. This report is the first step toward understanding the species-level specificity of the interaction between perilla plants and Methylobacterium species.

    DOI: 10.1271/bbb.130207

    Web of Science

    PubMed

    researchmap

  • Assessment of change in biofilm architecture by nutrient concentration using a multichannel microdevice flow system 査読

    Zoe Sanchez, Akio Tani, Nobuhiro Suzuki, Reiko Kariyama, Hiromi Kumon, Kazuhide Kimbara

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   115 ( 3 )   326 - 331   2013年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC BIOSCIENCE BIOENGINEERING JAPAN  

    A new multichannel microdevice flow system with stainless steel flow chamber was used for architecture visualization, development monitoring and structural quantification of GFP-labeled Pseudomonas aeruginosa PAO1 live biofilms. Direct in situ investigations using confocal laser scanning microscopy (CLSM) at 72 h revealed structural pattern differences as a result of nutrient concentration gradients. When grown in LB medium, round, dispersed cellular aggregates were formed whereas in 1/3-diluted LB medium, biofilms were mostly flat and compact. However, COMSTAT analyses showed no considerable differences in biomass and thickness between the two LB concentrations. Characterization of time-dependent development of biofilms grown in 1/3-diluted LB medium showed full maturation of colonies by 120 h reaching maximum biomass at 17.1 mu m(3)/mu m(2) and average thickness at 44.4 mu m. Consequent thinning and formation of openings through interior in colonies occurred by 168 h. These results suggest that the new system tested allowed a fast and thick biofilm development on the surface of the stainless steel flow chamber. These findings may provide better estimates of biofilm activity and systematic evaluation of the effects of different parameters on biofilm morphology and development in industrial and biomedical systems. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.

    DOI: 10.1016/j.jbiosc.2012.09.018

    Web of Science

    PubMed

    researchmap

  • Extensive Reduction of Cell Viability and Enhanced Matrix Production in Pseudomonas aeruginosa PAO1 Flow Biofilms Treated with a D-Amino Acid Mixture 査読 国際誌

    Zoe Sanchez, Akio Tani, Kazuhide Kimbara

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   79 ( 4 )   1396 - 1399   2013年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    Treatment of Pseudomonas aeruginosa PAO1 flow biofilms with a D-amino acid mixture caused significant reductions in cell biomass by 75% and cell viability by 71%. No biofilm disassembly occurred, and matrix production increased by 30%, thereby providing a thick protective cover for remaining viable or persister cells.

    DOI: 10.1128/AEM.02911-12

    Web of Science

    PubMed

    researchmap

  • Methylobacterium haplocladii sp. nov. and Methylobacterium brachythecii sp. nov., isolated from bryophytes 査読 国際誌

    Akio Tani, Nurettin Sahin

    International Journal of Systematic and Evolutionary Microbiology   63 ( 9 )   3287 - 3292   2013年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MICROBIOLOGY SOC  

    Pink-pigmented, facultatively methylotrophic bacteria, strains 87eT and 99bT, were isolated from the bryophytes Haplocladium microphyllum and Brachythecium plumosum, respectively. The cells of both strains were Gram-reaction-negative, motile, non-spore-forming rods. On the basis of 16S rRNA gene sequence similarity, strains 87eT and 99bT were found to be related to Methylobacterium organophilum ATCC 27886T (97.1 % and 97.7%, respectively). Strains 87eT and 99bT showed highest 16S rRNA gene similarity to Methylobacterium gnaphalii 23eT (98.3 and 99.0%, respectively). The phylogenetic similarities to all other species of the genus Methylobacterium with validly published names were less than 97%. Major cellular fatty acids of both strains were C18:1ω7c and C18:0. The results of DNA-DNA hybridization, phylogenetic analyses based on 16S rRNA and cpn60 gene sequences, fatty acid profiles, whole-cell matrix- assisted, laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) analysis, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strains 87eT and 99bT from their phylogenetically closest relatives. We propose that strains 87eT and 99bT represent novel species within the genus Methylobacterium, for which the names Methylobacterium haplocladii sp. nov. (type strain 87eT=DSM 24195T=NBRC 107714T) and Methylobacterium brachythecii sp. nov. (type strain 99bT=DSM 24105T=NBRC 107710T) are proposed. © 2013 IUMS.

    DOI: 10.1099/ijs.0.048215-0

    Web of Science

    Scopus

    PubMed

    researchmap

  • Evaluation of rhizosphere, rhizoplane and phyllosphere bacteria and fungi isolated from rice in Kenya for plant growth promoters 査読 国際誌

    Mwashasha Rashid Mwajita, Hunja Murage, Akio Tani, Esther M. Kahangi

    SpringerPlus   2 ( 1 )   1 - 9   2013年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER INTERNATIONAL PUBLISHING AG  

    Rice (Oryza sativa L.) is the most important staple food crop in many developing countries, and is ranked third in Kenya after maize and wheat. Continuous cropping without replenishing soil nutrients is a major problem in Kenya resulting to declining soil fertility. The use of chemical fertilizers to avert the problem of low soil fertility is currently limited due to rising costs and environmental concerns. Many soil micro-organisms are able to solubilize the unavailable phosphorus, increase uptake of nitrogen and also synthesize growth promoting hormones including auxin. The aim of this study was to isolate and characterize phyllosphere, rhizoplane and rhizosphere micro-organisms from Kenyan rice with growth promoting habits. In this study whole plant rice samples were collected from different rice growing regions of Kenya. 76.2%, over 80% and 38.5% of the bacterial isolates were positive for phosphate solubilization, nitrogenase activity and IAA production whereas 17.5% and 5% of the fungal isolates were positive for phosphate solubilization and IAA production respectively. Hence these micro-organisms have potential for utilization as bio-fertilizers in rice production. © 2013 Mwajita et al.
    licensee Springer.

    DOI: 10.1186/2193-1801-2-606

    Web of Science

    Scopus

    PubMed

    researchmap

  • Mangrove sediment, a new source of potential biosurfactant-producing bacteria 査読

    Atipan Saimmai, Akio Tani, Vorasan Sobhon, Suppasil Maneerat

    ANNALS OF MICROBIOLOGY   62 ( 4 )   1669 - 1679   2012年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    Biosurfactant-producing bacteria were isolated from mangrove sediment samples collected in the southern part of Thailand by an enrichment-culture technique in which lubricating oil was the sole carbon source. A total of 1,600 colonies were obtained, which were screened for biosurfactant production using the qualitative drop-collapsing test in a mineral salts medium containing 1% of different carbon sources (commercial sugar, glucose, molasses, and used lubricating oil). Ninety-five isolates were positive for biosurfactant production based on the results of this test, among which 20 could reduce the surface tension of the 48-h culture supernatant. The phylogenetic position of these 20 isolates was evaluated by 16S rRNA gene sequence analysis. The production of biosurfactants was determined for strains representative of eight different bacterial genera. Leucobacter komagatae 183, one of the newly isolated strains showing biosurfactant production, produced extracellular biosurfactants which reduced the surface tension of the culture supernatant from 72.0 to 32.0 m/Nm. Eighteen strains released extracellular emulsifiers able to stabilize the emulsion formed. Among these, the strains L. komagatae 183 and Ochrobactrum anthropi 11/6 exhibited emulsification activities comparable to those of synthetic surfactants. Overall, the new biosurfactant-producing strains isolated in this study display promising features for the future development and use in economically efficient industrial-scale biotechnological processes.

    DOI: 10.1007/s13213-012-0424-9

    Web of Science

    researchmap

  • Methylobacterium gnaphalii sp nov., isolated from leaves of Gnaphalium spicatum 査読 国際誌

    Akio Tani, Nurettin Sahin, Kazuhide Kimbara

    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY   62 ( Pt 11 )   2602 - 2607   2012年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    A pink-pigmented, facultatively methylotrophic bacterium, strain 23e(T), was isolated from the leaves of Gnaphalium spicatum (cudweed). The cells of strain 23e(T) were Gram-reaction negative, motile and non-spore-forming rods. On the basis of 16S rRNA gene sequence similarities, strain 23e(T) was related to Methylobacterium organophilum ATCC 27886(T) (97.1 %) and Methylobacterium marchantiae JT1(T) (97 %), and the phylogenetic similarities to all other Methylobacterium species with validly published names were less than 97%. Major cellular fatty acids were C(18:1 omega)7c, C-16:00 and C-18:0. The results of DNA DNA hybridization, phylogenetic analyses based on 16S rRNA and cpn60 gene sequences, fatty acid profiles, whole-cell matrix-assisted laser desorption/ionization time of flight/MS analysis, physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 23e(T) from the phylogenetically closest relatives. We propose that strain 23e(T) represents a novel species within the genus Methylobacterium, for which the name Methylobacterium gnaphalii sp. nov. is proposed. The type strain is 23e(T) (=DSM 24027(T)=NBRC 107716(T)).

    DOI: 10.1099/ijs.0.037713-0

    Web of Science

    PubMed

    researchmap

  • Isolation and characterization of Kluyvera georgiana strain with the potential for acrylamide biodegradation 査読 国際誌

    Uthumporn Thanyacharoen, Akio Tani, Jittima Charoenpanich

    JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING   47 ( 11 )   1491 - 1499   2012年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS INC  

    Worldwide contamination by acrylamide, a neurotoxicant and carcinogen in animals, is becoming a significant problem. We isolated three novel acrylamide-degrading bacteria from domestic wastewater in Chonburi, Thailand. Using biochemical characteristics and 16S rRNA gene sequencing, the strains were identified as Klebsiella pneumoniae, Kluyvera georgiana and Enterococcus faecalis. K. georgiana strain No. 2 was selected for further characterization due to its degradation potential of high concentrations of acrylamide at the mesophilic temperatures. The strain grew well in the presence of acrylamide at concentrations to 0.5 % (w/v), pH 5.0 to 7.0 and 37 degrees C. Degradation of acrylamide to acrylic acid began after 30 min of cultivation as a biomass-dependent manner. Mass balance analysis revealed 92.3 % conversion of acrylamide to acrylic acid and two lower polarity compounds. Strain No. 2 degraded many aliphatic amides but not iodoacetamide and thioacetamide. High degradation level (&gt;80 %) was found with propionamide, cyanoacetamide and acetamide. Moderate degradation was obtained in the order of formamide &gt; butyramide &gt; lactamide &gt; urea while sodium azide provided 34% degradation. These findings render this novel bacterium attractive for biodegradation of acrylamide and other aliphatic amides in the environment.

    DOI: 10.1080/10934529.2012.680312

    Web of Science

    PubMed

    researchmap

  • Isolation and screening of lactic acid bacteria from Thai traditional fermented fish (Plasom) and production of Plasom from selected strains 査読

    Noraphat Hwanhlem, Subaidah Buradaleng, Saowakon Wattanachant, Soottawat Benjakul, Akio Tani, Suppasil Maneerat

    FOOD CONTROL   22 ( 3-4 )   401 - 407   2011年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI LTD  

    Lactic acid bacteria (LAB) were isolated from traditional Thai fermented fish, Plasom at various fermentation periods. It was found that 138 isolates exhibited a clear zone and growth on MRS agar supplemented with CaCO3; however, only 133 isolates were identified as LAB. Only 14 strains showed excellent inhibition zone diameters on agar when Salmonella sp. was used as an indicator for preliminary detection of antagonistic activity. Staphylococcus aureus was used for secondary screening for antagonistic activity of these 14 strains. It was found that only 7 strains exhibited good inhibition zone diameters on agar, and all of them could inhibit E. coli as the third indicator. The strains which exhibited widest zones of inhibition against Escherichia coli, S. aureus and Salmonella sp. were LPS04, LPS17, and LD219 and LPS18 respectively. Tested pathogenic strains were inhibited by 4 selected LAB. The strain which showed the best lactic acid production and pH reduction ability was LD219. Using 16s rDNA sequence analysis, LD219 was identified as Streptococcus salivarius, LPS04. LPS17 and LPS18 were identified as Enterococcus faecalis. Plasom was produced by using a mixed culture (LD219, LPS04, LPS17 and LPS18) as a starter culture compared with spontaneous and back-slopping processes. Significant differences (p &lt; 0.05) in pH, its titratable acidity as lactic acid and number of total viable counts (TVC), LAB and Enterobacteriaceae were found in these Plasom at 0 and 8 days. However, no significant difference (p &gt; 0.05) was observed in terms of colour, smell, taste, sour, texture and overall acceptance of Plasm produced by non-starter cultured, back-slopping and starter cultured processes. (C) 2010 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.foodcont.2010.09.010

    Web of Science

    researchmap

  • Rapid and multiple in situ identification and analyses of physiological status of specific bacteria based on fluorescent in situ hybridization 査読

    Xian Zhang, Akio Tani, Fusako Kawai, Kazuhide Kimbara

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   110 ( 6 )   716 - 719   2010年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC BIOSCIENCE BIOENGINEERING JAPAN  

    Quantitative analysis of the target microorganism in microbial communities is important for the assessment of bacterial activity in environment. Here we present a method of a combination of fluorescent in situ hybridization (FISH) method and live/dead staining which allows in situ identification and analysis of physiological status of specific bacteria. (C) 2010, The Society for Biotechnology, Japan. All rights reserved.

    DOI: 10.1016/j.jbiosc.2010.07.004

    Web of Science

    PubMed

    researchmap

  • Transcription factor Stb5p is essential for acetaldehyde tolerance in Saccharomyces cerevisiae 査読 国際誌

    Yoshimi Matsufuji, Tomoyuki Nakagawa, Shuki Fujimura, Akio Tani, Junichi Nakagawa

    JOURNAL OF BASIC MICROBIOLOGY   50 ( 5 )   494 - 498   2010年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-V C H VERLAG GMBH  

    Transcription factor Stb5p, previously known as one of the multidrug resistance gene regulators in Saccharomyces cerevisiae, was shown here to play an essential role in acetaldehyde tolerance. A mutant strain, Delta stb5 exhibited increased acetaldehyde sensitivity, and failed to induce genes such as GND1, TKL1 and TAL1 involved in the pentose phosphate pathway (PPP) upon acetaldehyde stress. Using this strain it was revealed that Stb5p acts as a repressor for PGI1 encoding glucose-6-phosphate isomerase under acetaldehyde stress. In reverse, overexpression of Stb5p reinforced acetaldehyde tolerance to the yeast. Furthermore, various deletion mutants of the genes involved in glycolysis showed increased acetaldehyde tolerance compared to the wild-type strain. From these results, it was suggested that Stb5p participates in acetaldehyde tolerance by regulating expression of the PPP genes and PGI1, and that down-regulation of glycolytic pathway may lead to vitalization of PPP and to increased acetaldehyde tolerance.

    DOI: 10.1002/jobm.200900391

    Web of Science

    PubMed

    researchmap

  • A Bacterial Biosensor for Oxidative Stress Using the Constitutively Expressed Redox-Sensitive Protein roGFP2 査読 国際誌

    Carlos R. Arias-Barreiro, Keisuke Okazaki, Apostolos Koutsaftis, Salmaan H. Inayat-Hussain, Akio Tani, Maki Katsuhara, Kazuhide Kimbara, Izumi C. Mori

    SENSORS   10 ( 7 )   6290 - 6306   2010年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MDPI AG  

    A highly specific, high throughput-amenable bacterial biosensor for chemically induced cellular oxidation was developed using constitutively expressed redox-sensitive green fluorescent protein roGFP2 in E. coli (E. coli-roGFP2). Disulfide formation between two key cysteine residues of roGFP2 was assessed using a double-wavelength ratiometric approach. This study demonstrates that only a few minutes were required to detect oxidation using E. coli-roGFP2, in contrast to conventional bacterial oxidative stress sensors. Cellular oxidation induced by hydrogen peroxide, menadione, sodium selenite, zinc pyrithione, triphenyltin and naphthalene became detectable after 10 seconds and reached the maxima between 80 to 210 seconds, contrary to Cd(2+), Cu(2+), Pb(2+), Zn(2+) and sodium arsenite, which induced the oxidation maximum immediately. The lowest observable effect concentrations (in ppm) were determined as 1.0 x 10(-7) (arsenite), 1.0 x 10(-4) (naphthalene), 1.0 x 10(-4) (Cu(2+)), 3.8 x 10(-4) (H(2)O(2)), 1.0 x 10(-3) (Cd(2+)), 1.0 x 10(-3) (Zn(2+)), 1.0 x 10(-2) (menadione), 1.0 (triphenyltin), 1.56 (zinc pyrithione), 3.1 (selenite) and 6.3 (Pb(2+)), respectively. Heavy metal-induced oxidation showed unclear response patterns, whereas concentration-dependent sigmoid curves were observed for other compounds. In vivo GSH content and in vitro roGFP2 oxidation assays together with E. coli-roGFP2 results suggest that roGFP2 is sensitive to redox potential change and thiol modification induced by environmental stressors. Based on redox-sensitive technology, E. coli-roGFP2 provides a fast comprehensive detection system for toxicants that induce cellular oxidation.

    DOI: 10.3390/s100706290

    Web of Science

    PubMed

    researchmap

  • Flow cytometry-based method for isolating live bacteria with meta-cleavage activity on dihydroxy compounds of biphenyl 査読

    Sou Iijima, Yumi Shimomura, Yousuke Haba, Fusako Kawai, Akio Tani, Kazuhide Kimbara

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   109 ( 6 )   645 - 651   2010年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC BIOSCIENCE BIOENGINEERING JAPAN  

    A new method for isolating targeted live bacterial cells was established with the use of cell sorting by flow cytometry (FCM) based on the fluorescence of the intermediate metabolite of biphenyl degradation. During biphenyl degradation, a PCB degrader, Comamonas testosteroni 02, produces a meta-cleavage intermediate metabolite, 2-hydroxy-6-oxo-6-phenylhexa-2, 4-dienoic acid (HOPDA), which emits green fluorescence. HOPDA was produced from 2,3-dihydroxy biphenyl as a substrate, but it was not appropriate for labeling cells because it was released from the cells into the medium. When we used 4-n-butylbiphenyl and 4-n-heptylbiphenyl, we found that the cells produced and accumulated 2,3-dihydroxy intermediate metabolites. By the addition of synthesized 2,3-dihydroxy-4&apos;-butylbiphenyl (2,3-DHBBP), we were able to label the cells with strong green fluorescence, suggesting the persistence of fluorescent intermediate metabolite in the cells by the introduction of the alkyl tail. 2,3-DHBBP was then used to label strain TK102 and the cells were sorted with FCM. The sorting efficiency of FCM was defined as the percentage of colony numbers per sorting events. Strain TK102 cells were successfully enriched by 4.1-fold from the mixture with environmental indigenous bacteria with a sorting efficiency of 7.3%. The method we present here serves as a basic technique for the specific and direct isolation of live bacterial cells which contain dioxygenases active on dihydroxylated aromatic compounds. (C) 2009, The Society for Biotechnology. japan. All rights reserved.

    DOI: 10.1016/j.jbiosc.2009.11.023

    Web of Science

    PubMed

    researchmap

  • Significance of fatal head injuries associated with subependymal hemorrhage at the anterior horns of lateral ventricles 査読 国際誌

    Naohito Kuroda, Sohtaro Mimasaka, Takeshi Kita, Akio Tani

    Legal Medicine   11 ( 1 )   S180 - S181   2009年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We hypothesize the following items based on 41 autopsy cases with or without head injuries especially on subependymal injuries at the anterior horns of the lateral cerebral ventricles. The subependymal injuries at the regions (1) are not seen in fatal cases that did not have intracranial injuries, (2) have nothing to do with technical procedures for removal of the whole brain at autopsy, (3) are frequently associated with axonal injuries, (4) are presumed to be formed by rotating head injuries in a sagittal direction. © 2009 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.legalmed.2009.02.061

    Scopus

    PubMed

    researchmap

  • The crucial role of mitochondrial regulation in adaptive aluminium resistance in Rhodotorula glutinis 査読 国際誌

    Akio Tani, Chiemi Inoue, Yoko Tanaka, Yoko Yamamoto, Hideki Kondo, Syuntaro Hiradate, Kazuhide Kimbara, Fusako Kawai

    MICROBIOLOGY-SGM   154 ( Pt 11 )   3437 - 3446   2008年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    Rhodotorula glutinis IFO1125 was found to acquire increased aluminium (Al) resistance from 50 mu M to more than 5 mM by repetitive culturing with stepwise increases in Al concentration at pH 4.0. To investigate the mechanism underlying this novel phenomenon, wild-type and Al- resistant cells were compared. Neither cell type accumulated the free form of Al (Al3+) added to the medium. Transmission electron microscopic analyses revealed a greater number of mitochondria in resistant cells. The formation of small mitochondria with simplified cristae structures was observed in the wild-type strain grown in the presence of Al and in resistant cells grown in the absence of Al. Addition of Al to cells resulted in high mitochondrial membrane potential and concomitant generation of reactive oxygen species (ROS). Exposure to Al also resulted in elevated levels of oxidized proteins and oxidized lipids. Addition of the antioxidants a-tocopherol and ascorbic acid alleviated the Al toxicity, suggesting that ROS generation is the main cause of Al toxicity. Differential display analysis indicated upregulation of mitochondrial genes in the resistant cells. Resistant cells were found to have 2.5- to 3-fold more mitochondrial DNA (mtDNA) than the wild-type strain. Analysis of tricarboxylic acid cycle and respiratory-chain enzyme activities in wild-type and resistant cells revealed significantly reduced cytochrome c oxidase activity and resultant high ROS production in the latter cells. Taken together, these data suggest that the adaptive increased resistance to Al stress in resistant cells resulted from an increased number of mitochondria and increased mtDNA content, as a compensatory response to reduced respiratory activity caused by a deficiency in complex IV function.

    DOI: 10.1099/mic.0.2007/016048-0

    Web of Science

    PubMed

    researchmap

  • Proposed oxidative metabolic pathway for polypropylene glycol in Sphingobium sp strain PW-1 査読 国際誌

    Xiaoping Hu, Xin Liu, Akio Tani, Kazuhide Kimbara, Fusako Kawai

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   72 ( 4 )   1115 - 1118   2008年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Polypropylene glycol (PPG)-assimilating Sphingobium sp. strain PW-1 was grown on 0.5% PPG 700. PPG and its metabolites were analyzed by MALDI-TOF mass spectrometry. An oxidized form of a terminal alcohol group appeared with each molecular species as a metabolite. Cell-free extract showed PPG dehydrogenase activity. In this way, the oxidative metabolic pathway for PPG was confirmed.

    DOI: 10.1271/bbb.70749

    Web of Science

    PubMed

    researchmap

  • Structure and conservation of a polyethylene glycol-degradative operon in sphingomonads 査読 国際誌

    Akio Tani, Jittima Charoenpanich, Terumi Mori, Mayuko Takeichi, Kazuhide Kimbara, Fusako Kawai

    MICROBIOLOGY-SGM   153 ( Pt 2 )   338 - 346   2007年2月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    Sphingopyxis terrae, and Sphingopyxis macrogoltabida strains 103 and 203, can degrade polyethylene glycols (PEGs). They differ in the following respects: (i) different substrate specificities (chain length) of assimilable PEG, (ii) PEG-inducible or constitutive PEG-degradative proteins, and (iii) symbiotic or axenic degradation of PEG. S. terrae was able to incorporate PEG 6000, but strain 103 could not incorporate more than PEG 4000, suggesting that the difference in assimilable PEG chain length depends on the ability to take up substrate. PEG-degradative genes (pegB, C, D, A, E and R) from these strains were cloned. Their primary structures shared a high homology of more than 99%. The peg genes encode a TonB-dependent receptor (pegB), a PEG-aldehyde dehydrogenase (pegC), a permease (pegD), a PEG dehydrogenase (pegA) and an acyl-CoA ligase (pegE), and in the opposite orientation, an AraC-type transcription regulator (pegR). The peg operon was flanked by two different sets of transposases. These three strains contained large plasmids and the operon was located in one of the large plasmids in S. terrae. The peg genes could be detected in other PEG-degrading sphingomonads. These results suggest that the peg genes have evolved in a plasmid-mediated manner. An insertion of a transposon gene (pegF) between pegD and pegA in strain 203 was found, which caused the constitutive expression of pegA in this strain.

    DOI: 10.1099/mic.0.2006/000992-0

    Web of Science

    PubMed

    researchmap

  • Cloning and expression of the gene for periplasmic poly(vinyl alcohol) dehydrogenase from Sphingomonas sp strain 113P3, a novel-type quinohaemoprotein alcohol dehydrogenase 査読 国際誌

    Rie Hirota-Mamoto, Ryoko Nagai, Shinjiro Tachibana, Masaaki Yasuda, Akio Tani, Kazuhide Kimbara, Fusako Kawai

    MICROBIOLOGY-SGM   152 ( Pt 7 )   1941 - 1949   2006年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    A gene for periplasmic poly(vinyl alcohol) (PVA) dehydrogenase (PVADH) was cloned, based on the N-terminal amino acid sequence of the purified PVADH from Sphingomonas sp. 113P3 and the sequence of the gene for PVADH (pvaA, GenBank accession no. AB 190288). The recombinant PVADH tagged with hexahistidine was expressed in Escherichia coli and purified to homogeneity. The recombinant enzyme had the same characteristics as the purified enzyme from Sphingomonas sp. strain 113P. In addition to PVA, the recombinant PVADH could oxidize glycols such as polypropylene glycols and 1,3-butane/cyclohexanediol and 2,4-pentanediol, but neither primary nor secondary alcohols. The amino acid sequence of the recombinant PVADH showed similarity with those of PVADH from Pseudomonas sp. strain VM15C, putative PVADHs from Azoarcus sp. EbN1, and Xanthomonas species (54-25% identity), and the quinohaemoprotein alcohol dehydrogenases (OH-ADHs) from Comamonas testosteroni, Ralstonia eutropha and Pseudomonas putida (25-29% identity). PVADHs from strains 113P3 and VM15C have a conserved superbarrel domain (SD), probable PQQ-binding amino acids in the SID and a haem-binding domain (HBD) (they should be designated QH-PVADHs), but the positions of the amino acid sequences for the HBD and SID are the reverse of those of OH-ADHs. A protein structure of QH-PVADHs is proposed. Results of dot-blot hybridization and RT-PCR indicated that the three genes encoding oxidized PVA hydrolase, PVADH and cytochrome c are expressed constitutively and form an operon.

    DOI: 10.1099/mic.0.28848-0

    Web of Science

    PubMed

    researchmap

  • Analysis of amino acid residues involved in catalysis of polyethylene glycol dehydrogenase from Sphingopyxis terrae, using three-dimensional molecular modeling-based kinetic characterization of mutants 査読 国際誌

    T Ohta, T Kawabata, K Nishikawa, A Tani, K Kimbara, F Kawai

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   72 ( 6 )   4388 - 4396   2006年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    Polyethylene glycol dehydrogenase (PEGDH) from Sphingopyxis terrae (formerly Sphingomonas terrae) is composed of 535 amino acid residues and one flavin adenine dinucleotide per monomer protein in a homodimeric structure. Its amino acid sequence shows 28.5 to 30.5% identity with glucose oxidases from Aspergillus niger and Penicillium amagasakiense. The ADP-binding site and the signature 1 and 2 consensus sequences of glucose-methanol-choline oxidoreductases are present in PEGDH. Based on three-dimensional molecular modeling and kinetic characterization of wild-type PEGDH and mutant PEGDHs constructed by site-directed mutagenesis, residues potentially involved in catalysis and substrate binding were found in the vicinity of the flavin ring. The catalytically important active sites were assigned to His-467 and Asn-511. One disulfide bridge between Cys-379 and Cys-382 existed in PEGDH and seemed to play roles in both substrate binding and electron mediation. The Cys-297 mutant showed decreased activity, suggesting the residue's importance in both substrate binding and electron mediation, as well as Cys-379 and Cys-382. PEGDH also contains a motif of a ubiquinone-binding site, and coenzyme Q(10) was utilized as an electron acceptor. Thus, we propose several important amino acid residues involved in the electron transfer pathway from the substrate to ubiquinone.

    DOI: 10.1128/AEM.02174-05

    Web of Science

    PubMed

    researchmap

  • Biochemical and molecular characterization of a periplasmic hydrolase for oxidized polyvinyl alcohol from Sphingomonas sp strain 113P3 査読 国際誌

    W Klomklang, A Tani, K Kimbara, R Mamoto, T Ueda, M Shimao, F Kawai

    MICROBIOLOGY-SGM   151 ( Pt 4 )   1255 - 1262   2005年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    Oxidized polyvinyl alcohol hydrolase (OPH) and polyvinyl alcohol dehydrogenase were found to be constitutively present in the periplasm of Sphingromonas sp. strain 113P3 (formerly Pseudomonas sp. 113P3). The OPH was purified to homogeneity with a yield of 40% and a 5-9-fold increase in specific activity. The enzyme was a homodimer consisting of 35 kDa subunits. Its activity was inhibited by PMSF, Hg2+ and Zn2+ . The enzyme hydrolysed oxidized polyvinyl alcohol (oxidized PVA) and p-nitrophenyl acetate (PNPA), but did not hydrolyse any of the mono- or diketones tested. K-m and V-max, values for oxidized PVA and PNPA were 0-2 and 0(.)3 mM, and 0(.)1 and 3(.)4 mu mol min(-1) mg(-1), respectively. The gene for OPH was cloned and sequenced. Sequencing analysis revealed that the open reading frame consisted of 1095 bp, corresponding to a protein of 364 amino acids residues, encoding a signal peptide and a mature protein of 34 and 330 amino acids residues, respectively. The presence of a serine-hydrolase motif (a lipase box; Gly-X-Ser-X-Gly) strongly suggested that the enzyme belongs to the serine-hydrolase family. The protein exhibited homology with OPH of the Pseudomonas sp. strain VM15C (63% identity) and the polyhydroxybutyrate depolymerases from Mesorhizobium loti, Rhizobium sp. and Sinorhizobium meliloti (29-32% identity). The oph gene was expressed in Escherichia coli under the control of the lac promoter. The recombinant protein had the same molecular mass and N-terminal amino acid sequence as the purified OPH from strain 113P3.

    DOI: 10.1099/mic.0.27655-0

    Web of Science

    PubMed

    researchmap

▼全件表示

書籍等出版物

  • Degradable polymers and materials

    ACS  2006年 

     詳細を見る

MISC

  • Cultivable Methylobacterium species diversity in rice seeds identified with whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis

    Marie Okumura, Yoshiko Fujitani, Masahiko Maekawa, Jittima Charoenpanich, Hunja Murage, Kazuhide Kimbara, Nurettin Ahin, Akio Tani

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   123 ( 2 )   190 - 196   2017年2月

     詳細を見る

    担当区分:責任著者   記述言語:英語   出版者・発行元:SOC BIOSCIENCE BIOENGINEERING JAPAN  

    Methylobacterium species are methylotrophic bacteria that widely inhabit plant surfaces. In addition to studies on methylotrophs as model organisms, research has also been conducted on their mechanism of plant growth promotion as well as the species-species specificity of plant-microbe interaction. We employed whole-cell matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (WC-MS) analysis, which enables the rapid and accurate identification of bacteria at the species level, to identify Methylobacterium isolates collected from the rice seeds of different cultivars harvested in Japan, Thailand, and Kenya. Rice seeds obtained from diverse geographical locations showed different communities of Methylobacterium species. We found that M. fujisawaense, M. aquaticum, M. platani, and M. radiotolerans are the most frequently isolated species, but none were isolated as common species from 18 seed samples due to the highly biased communities in some samples. These findings will contribute to the development of formulations containing selected species that promote rice growth, though it may be necessary to customize the formulations depending on the cultivars and farm conditions. (C) 2016, The Society for Biotechnology, Japan. All rights reserved.

    DOI: 10.1016/j.jbiosc.2016.09.001

    Web of Science

    PubMed

    researchmap

  • Selective growth promotion of bloom-forming raphidophyte Heterosigma akashiwo by a marine bacterial strain 国際誌

    Aiko Higashi, Yoshiko Fujitani, Natsuko Nakayama, Akio Tani, Shoko Ueki

    HARMFUL ALGAE   60   150 - 156   2016年12月

     詳細を見る

    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    Algal bloom is typically caused by aberrant propagation of a single species, resulting in its predomination in the local population. While environmental factors including temperature and eutrophication are linked to bloom, the precise mechanism of its formation process is still obscure. Here, we isolated a bacterial strain that promotes growth of Heterosigma akashiwo, a Raphidophyceae that causes harmful algal blooms. Based on 16S rRNA gene sequence, the strain was identified as Altererythrobacter ishigakiensis, a member of the class Alphaproteobacteria. When added to culture, this strain facilitated growth of H. akashiwo and increased its cell culture yield significantly. Importantly, this strain did not affect the growth of other raphidophytes, Chattonella ovate and C. antiqua, indicating that it promotes growth of H. akashiwo in a species-specific manner. We also found that, in co-culture, H. akashiwo suppressed the growth of C. ovate. When A. ishigakiensis was added to the mixed culture, H. akashiwo growth was facilitated while C ovate propagation was markedly suppressed, indicating that the presence of the bacterium enhances the dominance of H. akashiwo over C ovate. This is the first example of selective growth promotion of H. akashiwo by a marine bacterium, and may exemplify importance of symbiotic bacterium on algal bloom forming process in general. (C) 2016 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.hal.2016.11.009

    Web of Science

    PubMed

    researchmap

  • Complete genome sequence of Methylobacterium aquaticum strain 22A, isolated from a Racomitrium japonicum moss

    Akio Tani, Yoshitoshi Ogura, Tetsuya Hayashi, Kazuhide Kimbara

    Genome Announcements   3 ( 2 )   e00266-15   2016年

     詳細を見る

    記述言語:英語   出版者・発行元:American Society for Microbiology  

    Methylobacterium species colonize plant surfaces and utilize methanol emitted from plants. Methylobacterium aquaticum strain 22A was isolated from a hydroponic culture of a moss, Racomitrium japonicum, and is a potent plant growth promoter. The complete genome sequencing of the strain confirmed the presence of genes related to plant growth promotion and methylotrophy.

    DOI: 10.1128/genomeA.00266-15

    Scopus

    researchmap

  • Complete genome sequence of Methylobacterium aquaticum strain 22A, isolated from a Racomitrium japonicum moss 国際誌

    Akio Tani, Yoshitoshi Ogura, Tetsuya Hayashi, Kazuhide Kimbara

    Genome Announcements   3 ( 2 )   2016年

     詳細を見る

    記述言語:英語   出版者・発行元:American Society for Microbiology  

    Methylobacterium species colonize plant surfaces and utilize methanol emitted from plants. Methylobacterium aquaticum strain 22A was isolated from a hydroponic culture of a moss, Racomitrium japonicum, and is a potent plant growth promoter. The complete genome sequencing of the strain confirmed the presence of genes related to plant growth promotion and methylotrophy.

    DOI: 10.1128/genomeA.00266-15

    Scopus

    PubMed

    researchmap

  • Detection and preliminary characterization of a narrow spectrum bacteriocin produced by lactobacillus pentosus K2N7 from Thai traditional fermented shrimp (Kung-Som)

    Nisit Watthanasakphuban, Akio Tani, Soottawat Benjakul, Suppasil Maneerat

    Songklanakarin Journal of Science and Technology   38 ( 1 )   47 - 55   2016年

     詳細を見る

  • Production of ergothioneine by Methylobacterium species

    Kabir M. Alamgir, Sachiko Masuda, Yoshiko Fujitani, Fumio Fukuda, Akio Tani

    FRONTIERS IN MICROBIOLOGY   6   1185   2015年10月

     詳細を見る

    記述言語:英語   出版者・発行元:FRONTIERS MEDIA SA  

    Metabolomic analysis revealed that Methylobacterium cells accumulate a large amount of ergothioneine (EGT), which is a sulfur containing, non-proteinogenic, antioxidative amino acid derived from histidine. EGT biosynthesis and its role in methylotrophy and physiology for plant surface-symbiotic Methylobacterium species were investigated in this study. Almost all Methylobacterium type strains can synthesize FGT. We selected one of the most productive strains (M. aquaticum strain 22A isolated from a moss), and investigated the feasibility of fermentative EGT production through optimization of the culture condition. Methanol as a carbon source served as the best substrate for production. The productivity reached up to 1000 mu g/100 ml culture (1200 mu g/g wet weight cells, 6.3 mg/g dry weight) in 38 days. Next, we identified the genes (egtBD) responsible for EGT synthesis, and generated a deletion mutant defective in EGT production. Compared to the wild type, the mutant showed better growth on methanol and on the plant surface as well as severe susceptibility to heat treatment and irradiation of ultraviolet (UV) and sunlight. These results suggested that EGT is not involved in methylotrophy, but is involved in their phyllospheric lifestyle fitness of the genus in natural conditions.

    DOI: 10.3389/fmicb.2015.01185

    Web of Science

    researchmap

  • Production of ergothioneine by Methylobacterium species

    Kabir M. Alamgir, Sachiko Masuda, Yoshiko Fujitani, Fumio Fukuda, Akio Tani

    FRONTIERS IN MICROBIOLOGY   6   1185   2015年10月

     詳細を見る

    記述言語:英語   出版者・発行元:FRONTIERS MEDIA SA  

    Metabolomic analysis revealed that Methylobacterium cells accumulate a large amount of ergothioneine (EGT), which is a sulfur containing, non-proteinogenic, antioxidative amino acid derived from histidine. EGT biosynthesis and its role in methylotrophy and physiology for plant surface-symbiotic Methylobacterium species were investigated in this study. Almost all Methylobacterium type strains can synthesize FGT. We selected one of the most productive strains (M. aquaticum strain 22A isolated from a moss), and investigated the feasibility of fermentative EGT production through optimization of the culture condition. Methanol as a carbon source served as the best substrate for production. The productivity reached up to 1000 mu g/100 ml culture (1200 mu g/g wet weight cells, 6.3 mg/g dry weight) in 38 days. Next, we identified the genes (egtBD) responsible for EGT synthesis, and generated a deletion mutant defective in EGT production. Compared to the wild type, the mutant showed better growth on methanol and on the plant surface as well as severe susceptibility to heat treatment and irradiation of ultraviolet (UV) and sunlight. These results suggested that EGT is not involved in methylotrophy, but is involved in their phyllospheric lifestyle fitness of the genus in natural conditions.

    DOI: 10.3389/fmicb.2015.01185

    Web of Science

    researchmap

  • 緑膿菌(Pseudomonas aeruginosa)PAO1株バイオフィルムに対するD‐アミノ酸混合物の影響

    大田隼矢, サンチェス ゾイ, 青木真央, 土濱諒, 谷明生, 新谷政己, 金原和秀

    中部化学関係学協会支部連合秋季大会講演予稿集   44th   226   2013年11月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • D‐アミノ酸処理によるPseudomonas aeruginosa PAO1株バイオフィルムの構造変化の解析

    大田隼矢, ZOE Sanchez, 青木真央, 谷明生, 新谷政己, 金原和秀

    日本生物工学会大会講演要旨集   65th   235   2013年8月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • アカシソ種子由来Methylobacterium sp.OR‐01株のアカシソ上での動態

    水野雅之, 井口博之, 谷明生, 由里本博也, 阪井康能

    日本農芸化学会大会講演要旨集(Web)   2013   3B21A07 (WEB ONLY)   2013年3月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • Pseudomonas aeruginosa PAO1株バイオフィルムのD‐アミノ酸処理に対する挙動

    大田隼矢, SANCHEZ Zoe, 青木真央, 谷明生, 新谷政己, 金原和秀

    日本農芸化学会大会講演要旨集(Web)   2013   3B12P07 (WEB ONLY)   2013年3月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • Assessment of change in biofilm architecture by nutrient concentration using a multichannel microdevice flow system

    Zoe Sanchez, Akio Tani, Nobuhiro Suzuki, Reiko Kariyama, Hiromi Kumon, Kazuhide Kimbara

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   115 ( 3 )   326 - 331   2013年3月

     詳細を見る

    記述言語:英語   出版者・発行元:SOC BIOSCIENCE BIOENGINEERING JAPAN  

    A new multichannel microdevice flow system with stainless steel flow chamber was used for architecture visualization, development monitoring and structural quantification of GFP-labeled Pseudomonas aeruginosa PAO1 live biofilms. Direct in situ investigations using confocal laser scanning microscopy (CLSM) at 72 h revealed structural pattern differences as a result of nutrient concentration gradients. When grown in LB medium, round, dispersed cellular aggregates were formed whereas in 1/3-diluted LB medium, biofilms were mostly flat and compact. However, COMSTAT analyses showed no considerable differences in biomass and thickness between the two LB concentrations. Characterization of time-dependent development of biofilms grown in 1/3-diluted LB medium showed full maturation of colonies by 120 h reaching maximum biomass at 17.1 mu m(3)/mu m(2) and average thickness at 44.4 mu m. Consequent thinning and formation of openings through interior in colonies occurred by 168 h. These results suggest that the new system tested allowed a fast and thick biofilm development on the surface of the stainless steel flow chamber. These findings may provide better estimates of biofilm activity and systematic evaluation of the effects of different parameters on biofilm morphology and development in industrial and biomedical systems. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.

    DOI: 10.1016/j.jbiosc.2012.09.018

    Web of Science

    researchmap

  • Extensive Reduction of Cell Viability and Enhanced Matrix Production in Pseudomonas aeruginosa PAO1 Flow Biofilms Treated with a D-Amino Acid Mixture

    Zoe Sanchez, Akio Tani, Kazuhide Kimbara

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   79 ( 4 )   1396 - 1399   2013年2月

     詳細を見る

    記述言語:英語   出版者・発行元:AMER SOC MICROBIOLOGY  

    Treatment of Pseudomonas aeruginosa PAO1 flow biofilms with a D-amino acid mixture caused significant reductions in cell biomass by 75% and cell viability by 71%. No biofilm disassembly occurred, and matrix production increased by 30%, thereby providing a thick protective cover for remaining viable or persister cells.

    DOI: 10.1128/AEM.02911-12

    Web of Science

    researchmap

  • 1P-101 Methylobacterium extorquensのメタノール代謝におけるレアアースの特異性と役割(一般講演(発酵生理学,発酵工学))

    日比野 歩美, 三井 亮司, 谷 明生, 田代 晋也, 早川 享志, 中川 智行

    日本生物工学会大会講演要旨集   65   43 - 43   2013年

     詳細を見る

    記述言語:日本語   出版者・発行元:日本生物工学会  

    CiNii Article

    CiNii Books

    researchmap

  • A Catalytic Role of XoxF1 as La3+-Dependent Methanol Dehydrogenase in Methylobacterium extorquens Strain AM1 国際誌

    Tomoyuki Nakagawa, Ryoji Mitsui, Akio Tani, Kentaro Sasa, Shinya Tashiro, Tomonori Iwama, Takashi Hayakawa, Keiichi Kawai

    PLOS ONE   7 ( 11 )   e50480   2012年11月

     詳細を見る

    記述言語:英語   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    In the methylotrophic bacterium Methylobacterium extorquens strain AM1, MxaF, a Ca2+-dependent methanol dehydrogenase (MDH), is the main enzyme catalyzing methanol oxidation during growth on methanol. The genome of strain AM1 contains another MDH gene homologue, xoxF1, whose function in methanol metabolism has remained unclear. In this work, we show that XoxF1 also functions as an MDH and is La3+-dependent. Despite the absence of Ca2+ in the medium strain AM1 was able to grow on methanol in the presence of La3+. Addition of La3+ increased MDH activity but the addition had no effect on mxaF or xoxF1 expression level. We purified MDH from strain AM1 grown on methanol in the presence of La3+, and its N-terminal amino acid sequence corresponded to that of XoxF1. The enzyme contained La3+ as a cofactor. The Delta mxaF mutant strain could not grow on methanol in the presence of Ca2+, but was able to grow after supplementation with La3+. Taken together, these results show that XoxF1 participates in methanol metabolism as a La3+-dependent MDH in strain AM1.

    DOI: 10.1371/journal.pone.0050480

    Web of Science

    PubMed

    researchmap

  • Isolation and identification of endophytic bacteria of bananas (Musa spp.) in Kenya and their potential as biofertilizers for sustainable banana production

    Catherine Nyambura Ngamau, Viviene Njeri Matiru, Akio Tani, Catherine Wangari Muthuri

    AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH   6 ( 34 )   6414 - 6422   2012年9月

     詳細を見る

    記述言語:英語   出版者・発行元:ACADEMIC JOURNALS  

    This study was conducted with the aim of isolating and identifying endophytic bacteria associated with bananas in Kenya and assessing their functional potentiality as biological fertilizers. Banana material was collected from two different banana cultivars in five different geographical regions and bacteria were isolated using five different isolation media. Whole-cell matrix-assisted laser desorption/ ionization time of flight mass spectrometry (MALDI-TOF/MS) analysis was used for microorganism profiling. Protein from the living cells were extracted using the ethanol/formic acid extraction procedure and intact molecular weights of the ionized proteins directly measured and the pattern of the protein molecular weights used as fingerprints. Forty three isolates were selected for partial 16S rRNA gene sequencing. Isolates were characterized on the basis of their in-vitro plant growth-promoting activities that included abilities to fix free nitrogen, solubilize phosphates and produce siderophores. The isolates were identified as Serratia, Pseudomonas, Rahnella, Enterobacter, Raoultella, Yokenella, Bacillus, Klebsiella, Yersinia and Ewingella species. Siderophore production activity was detected with all the Pseudomonas isolates as determined on blue Chrome Azurol S (CAS) agar plates. Twenty seven isolates were observed to solubilize phosphates, with Rahnella isolates showing the highest potential as determined on NBRIP growth medium. All the isolates grew on solid nitrogen-source free medium, suggesting their ability to fix nitrogen. In conclusion, endophytic bacteria of bananas in Kenya were isolated and identified, and Rahnella and Pseudomonas isolates proposed as potential microbial biofertilizers for sustainable banana production in Kenya.

    DOI: 10.5897/AJMR12.1170

    Web of Science

    researchmap

  • High-Throughput Identification and Screening of Novel Methylobacterium Species Using Whole-Cell MALDI-TOF/MS Analysis 国際誌

    Akio Tani, Nurettin Sahin, Yumiko Matsuyama, Takashi Enomoto, Naoki Nishimura, Akira Yokota, Kazuhide Kimbara

    PLOS ONE   7 ( 7 )   e40784   2012年7月

     詳細を見る

    記述言語:英語   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Methylobacterium species are ubiquitous alpha-proteobacteria that reside in the phyllosphere and are fed by methanol that is emitted from plants. In this study, we applied whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis (WC-MS) to evaluate the diversity of Methylobacterium species collected from a variety of plants. The WC-MS spectrum was reproducible through two weeks of cultivation on different media. WC-MS spectrum peaks of M. extorquens strain AM1 cells were attributed to ribosomal proteins, but those were not were also found. We developed a simple method for rapid identification based on spectra similarity. Using all available type strains of Methylobacterium species, the method provided a certain threshold similarity value for species-level discrimination, although the genus contains some type strains that could not be easily discriminated solely by 16S rRNA gene sequence similarity. Next, we evaluated the WC-MS data of approximately 200 methylotrophs isolated from various plants with MALDI Biotyper software (Bruker Daltonics). Isolates representing each cluster were further identified by 16S rRNA gene sequencing. In most cases, the identification by WC-MS matched that by sequencing, and isolates with unique spectra represented possible novel species. The strains belonging to M. extorquens, M. adhaesivum, M. marchantiae, M. komagatae, M. brachiatum, M. radiotolerans, and novel lineages close to M. adhaesivum, many of which were isolated from bryophytes, were found to be the most frequent phyllospheric colonizers. The WC-MS technique provides emerging high-throughputness in the identification of known/novel species of bacteria, enabling the selection of novel species in a library and identification without 16S rRNA gene sequencing.

    DOI: 10.1371/journal.pone.0040784

    Web of Science

    PubMed

    researchmap

  • Methylobacterium oxalidis sp nov., isolated from leaves of Oxalis corniculata 国際誌

    Akio Tani, Nurettin Sahin, Kazuhide Kimbara

    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY   62 ( Pt 7 )   1647 - 1652   2012年7月

     詳細を見る

    記述言語:英語   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    A pink-pigmented, facultatively methylotrophic bacterium, strain 35a(T), was isolated from the leaves of Oxalis comiculata. Cells of strain 35a(T) were Gram-reaction-negative, motile, non-spore-forming rods. The highest 16S rRNA gene pairwise sequence similarities for strain 35a(T) were found with the strains of Methylobacterium iners 5317S-33(T) (96.7 %), 'Methylobacterium soil YIM 48816 (96.6%) and Methylobacterium jeotgali S2R03-9(T) (96.3 %). 16S rRNA gene sequence similarities with the type strains of all other recognized species of the genus Methylobacterium were below 96%. Major cellular fatty acids were C-18:1 omega 7c, C-18:0 and C-16:0, The results of DNA-DNA hybridization experiments, analysis of cpn60 gene sequences, fatty acid profiles, whole-cell MALDI-TOF/MS spectral pattern analysis, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 35a(T) from its nearest phylogenetic neighbours. Strain 35a(T) is therefore considered to represent a novel species within the genus Methylobacterium, for which the name Methylobacterium oxalidis sp. nov. is proposed. The type strain is 35a(T) (=DSM 24028(T) = NBRC 107715(T)).

    DOI: 10.1099/ijs.0.033019-0

    Web of Science

    PubMed

    researchmap

  • A novel quadripartite dsRNA virus isolated from a phytopathogenic filamentous fungus, Rosellinia necatrix 国際誌

    Yu-Hsin Lin, Sotaro Chiba, Akio Tani, Hideki Kondo, Atsuko Sasaki, Satoko Kanematsu, Nobuhiro Suzuki

    VIROLOGY   426 ( 1 )   42 - 50   2012年4月

     詳細を見る

    記述言語:英語   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Here we report the biological and molecular attributes of a novel dsRNA virus isolated from Rosellinia necatrix, a filamentous phytopathogenic fungus. The virus, termed Rosellinia necatrix quadrivirus 1 (RnQV1), forms rigid spherical particles approximately 45 nm in diameter in infected mycelia. The particles contain 4 dsRNA segments, dsRNA1 to dsRNA4, with a size range of 4.9 to 3.7 kbp, each possessing a single large ORF. A comparison of the virus-infected and -cured isogenic fungal strains suggested that RnQV1 infection has no appreciable phenotypic effects. Phylogenetic analysis using the dsRNA3-encoded RdRp sequence revealed that RnQV1 is more distantly related to quadripartite chrysoviruses than to monopartite totiviruses, and is placed in a distinct group from other mycoviruses. No significant sequence similarities were evident between known proteins and RnQV1 structural proteins shown to be encoded by dsRNA2 or dsRNA4. These suggest that RnQV1 is a novel latent virus, belonging to a new family. (C) 2012 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.virol.2012.01.013

    Web of Science

    PubMed

    researchmap

  • シソに生息するMethylobacterium属細菌の分布とその特性評価

    水野雅之, 井口博之, 谷明生, 由里本博也, 阪井康能

    日本農芸化学会大会講演要旨集(Web)   2012   2C23A06 (WEB ONLY)   2012年3月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • Practical Application of Methanol-Mediated Mutualistic Symbiosis between Methylobacterium Species and a Roof Greening Moss, Racomitrium 国際誌

    Akio Tani, Yuichiro Takai, Ikko Suzukawa, Motomu Akita, Haruhiko Murase, Kazuhide Kimbara

    PLOS ONE   7 ( 3 )   e33800   2012年3月

     詳細を見る

    記述言語:英語   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Bryophytes, or mosses, are considered the most maintenance-free materials for roof greening. Racomitrium species are most often used due to their high tolerance to desiccation. Because they grow slowly, a technology for forcing their growth is desired. We succeeded in the efficient production of R. japonicum in liquid culture. The structure of the microbial community is crucial to stabilize the culture. A culture-independent technique revealed that the cultures contain methylotrophic bacteria. Using yeast cells that fluoresce in the presence of methanol, methanol emission from the moss was confirmed, suggesting that it is an important carbon and energy source for the bacteria. We isolated Methylobacterium species from the liquid culture and studied their characteristics. The isolates were able to strongly promote the growth of some mosses including R. japonicum and seed plants, but the plant-microbe combination was important, since growth promotion was not uniform across species. One of the isolates, strain 22A, was cultivated with R. japonicum in liquid culture and in a field experiment, resulting in strong growth promotion. Mutualistic symbiosis can thus be utilized for industrial moss production.

    DOI: 10.1371/journal.pone.0033800

    Web of Science

    PubMed

    researchmap

  • Bacteriocin-producing lactic acid bacteria as a probiotic potential from Thai indigenous chickens

    H. Musikasang, N. Sohsomboon, A. Tani, S. Maneerat

    CZECH JOURNAL OF ANIMAL SCIENCE   57 ( 3 )   137 - 149   2012年3月

     詳細を見る

    記述言語:英語   出版者・発行元:CZECH ACADEMY AGRICULTURAL SCIENCES  

    Bacteriocin-producing lactic acid bacteria (LAB) were isolated and screened from the gastrointestinal tract (GIT) of Thai indigenous chickens. The bacteriocinogenic activities and the primary probiotic properties were determined. The bacteriocins produced by 14 strains of selected LAB displayed inhibitory activity against indicator strains after the supernatants were neutralized with NaOH in the following species: Lactobacillus sakei subsp. sakei JCM1157, Enterococcus faecalis VanB, Bacillus sp., and Listeria monocytogenes. The antagonistic activity of selected LAB was inactivated or decreased after being treated with proteolytic enzymes (alpha-chymotrypsin and trypsin). CR5-1 strain exhibited the highest level of activity (5120 AU/ml) in the stationary phase against L. sakei subsp. sakei JCM1157 in MRS broth at 37 degrees C. The nine isolates of selected LAB were investigated for primary probiotic properties. The survival of the nine isolates was found to decrease approximately by 3 log CFU/ml after passing through the gastrointestinal conditions. All isolates exhibited protein digestion on agar plates but no isolates showed the ability to digest starch and lipid. Most of them showed high susceptibilities to some antibiotics (penicillin G, tetracycline and erythromycin). Thirteen LAB strains producing bacteriocin with strongly inhibitory activity were identified as Lactobacillus salivarius and only one strain was identified by 16S rDNA sequence analysis as Lactobacillus agilis.

    Web of Science

    researchmap

  • Pandoraea oxalativorans sp nov., Pandoraea faecigallinarum sp nov and Pandoraea vervacti sp nov., isolated from oxalate-enriched culture 国際誌

    Nurettin Sahin, Akio Tani, Recep Kotan, Ivo Sedlacek, Kazuhide Kimbara, Abdurrahman U. Tamer

    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY   61 ( Pt 9 )   2247 - 2253   2011年9月

     詳細を見る

    記述言語:英語   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    Five isolates, designated TA2, TA4, TA25(T), KOx(T) and NS15(T) were isolated in previous studies by enrichment in mineral medium with potassium oxalate as the sole carbon source and were characterized using a polyphasic approach. The isolates were Gram-reaction-negative, aerobic, non-spore-forming rods. Phylogenetic analyses based on 16S rRNA and DNA gyrase B subunit (gyrB) gene sequences confirmed that the isolates belonged to the genus Pandoraea and were most closely related to Pandoraea sputorum and Pandoraea pnomenusa (97.2-99.70/0 16S rRNA gene sequence similarity). The isolates could be differentiated from their closest relatives on the basis of several phenotypic characteristics. The major cellular fatty acid profiles of the isolates comprised C(16:0), C(18:1)omega 7c, C(17:0) cyclo and summed feature 3 (C(16:1)omega 7c and/or iso-C(15:0) 2-OH). On the basis of DNA DNA hybridization studies and phylogenetic analyses, the isolates represent three novel species within the genus Pandoraea, for which the names Pandoraea oxalativorans sp. nov. (TA25(T) =NBRC 106091(T) =CCM 7677(T) =DSM 23570(T)), Pandoraea faecigallinarum sp. nov. (KOx(T) =NBRC 106092(T) =CCM 2766(T) =DSM 23572(T)) and Pandoraea vervacti sp. nov. (NS15(T) =NBRC 106088(T) =CCM 7667(T) =DSM 235711) are proposed.

    DOI: 10.1099/ijs.0.026138-0

    Web of Science

    PubMed

    researchmap

  • Culturable bacteria in hydroponic cultures of moss Racomitrium japonicum and their potential as biofertilizers for moss production

    Akio Tani, Motomu Akita, Haruhiko Murase, Kazuhide Kimbara

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   112 ( 1 )   32 - 39   2011年7月

     詳細を見る

    記述言語:英語   出版者・発行元:SOC BIOSCIENCE BIOENGINEERING JAPAN  

    The use of Racomitrium japonicum, a drought resistant bryophyte used for roof-greening, is gradually increasing. However, its utilization is hampered by slow growth rate. Here we isolated culturable bacteria from hydroponic cultivation samples to identify isolates that could promote moss growth. Most of the isolates belonged to Pseudomonas, Rhodococcus, and Duganella species. The isolates were biochemically characterized according to their type of interaction with plants, i.e., production of auxin, siderophores, or hydrogen cyanate, growth in the absence of an added nitrogen source, calcium phosphate solubilization, utilization of sugars, polymers, or aliphatic compounds, and antifungal activity. The isolates were applied to sterile protonemata and non-sterile adult gametophytes of R japonicum to evaluate their effect on plant growth. Furthermore, we isolated fungi that inhibited moss growth. Our results suggest that the microbial community structure in hydroponic cultures is important to stabilize moss production and the isolates that promote moss growth have potential to be utilized as biofertilizers for moss production. (C) 2011, The Society for Biotechnology, Japan. All rights reserved.

    DOI: 10.1016/j.jbiosc.2011.03.012

    Web of Science

    researchmap

  • Widespread Endogenization of Genome Sequences of Non-Retroviral RNA Viruses into Plant Genomes 国際誌

    Sotaro Chiba, Hideki Kondo, Akio Tani, Daisuke Saisho, Wataru Sakamoto, Satoko Kanematsu, Nobuhiro Suzuki

    PLOS PATHOGENS   7 ( 7 )   e1002146   2011年7月

     詳細を見る

    記述言語:英語   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Non-retroviral RNA virus sequences (NRVSs) have been found in the chromosomes of vertebrates and fungi, but not plants. Here we report similarly endogenized NRVSs derived from plus-, negative-, and double-stranded RNA viruses in plant chromosomes. These sequences were found by searching public genomic sequence databases, and, importantly, most NRVSs were subsequently detected by direct molecular analyses of plant DNAs. The most widespread NRVSs were related to the coat protein (CP) genes of the family Partitiviridae which have bisegmented dsRNA genomes, and included plant-and fungus-infecting members. The CP of a novel fungal virus (Rosellinia necatrix partitivirus 2, RnPV2) had the greatest sequence similarity to Arabidopsis thaliana ILR2, which is thought to regulate the activities of the phytohormone auxin, indole-3-acetic acid (IAA). Furthermore, partitivirus CP-like sequences much more closely related to plant partitiviruses than to RnPV2 were identified in a wide range of plant species. In addition, the nucleocapsid protein genes of cytorhabdoviruses and varicosaviruses were found in species of over 9 plant families, including Brassicaceae and Solanaceae. A replicase-like sequence of a betaflexivirus was identified in the cucumber genome. The pattern of occurrence of NRVSs and the phylogenetic analyses of NRVSs and related viruses indicate that multiple independent integrations into many plant lineages may have occurred. For example, one of the NRVSs was retained in Ar. thaliana but not in Ar. lyrata or other related Camelina species, whereas another NRVS displayed the reverse pattern. Our study has shown that single-and double-stranded RNA viral sequences are widespread in plant genomes, and shows the potential of genome integrated NRVSs to contribute to resolve unclear phylogenetic relationships of plant species.

    DOI: 10.1371/journal.ppat.1002146

    Web of Science

    PubMed

    researchmap

  • Widespread Endogenization of Genome Sequences of Non-Retroviral RNA Viruses into Plant Genomes

    Sotaro Chiba, Hideki Kondo, Akio Tani, Daisuke Saisho, Wataru Sakamoto, Satoko Kanematsu, Nobuhiro Suzuki

    PLOS PATHOGENS   7 ( 7 )   e1002146   2011年7月

     詳細を見る

    記述言語:英語   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Non-retroviral RNA virus sequences (NRVSs) have been found in the chromosomes of vertebrates and fungi, but not plants. Here we report similarly endogenized NRVSs derived from plus-, negative-, and double-stranded RNA viruses in plant chromosomes. These sequences were found by searching public genomic sequence databases, and, importantly, most NRVSs were subsequently detected by direct molecular analyses of plant DNAs. The most widespread NRVSs were related to the coat protein (CP) genes of the family Partitiviridae which have bisegmented dsRNA genomes, and included plant-and fungus-infecting members. The CP of a novel fungal virus (Rosellinia necatrix partitivirus 2, RnPV2) had the greatest sequence similarity to Arabidopsis thaliana ILR2, which is thought to regulate the activities of the phytohormone auxin, indole-3-acetic acid (IAA). Furthermore, partitivirus CP-like sequences much more closely related to plant partitiviruses than to RnPV2 were identified in a wide range of plant species. In addition, the nucleocapsid protein genes of cytorhabdoviruses and varicosaviruses were found in species of over 9 plant families, including Brassicaceae and Solanaceae. A replicase-like sequence of a betaflexivirus was identified in the cucumber genome. The pattern of occurrence of NRVSs and the phylogenetic analyses of NRVSs and related viruses indicate that multiple independent integrations into many plant lineages may have occurred. For example, one of the NRVSs was retained in Ar. thaliana but not in Ar. lyrata or other related Camelina species, whereas another NRVS displayed the reverse pattern. Our study has shown that single-and double-stranded RNA viral sequences are widespread in plant genomes, and shows the potential of genome integrated NRVSs to contribute to resolve unclear phylogenetic relationships of plant species.

    DOI: 10.1371/journal.ppat.1002146

    Web of Science

    researchmap

  • Culturable bacteria in hydroponic cultures of moss Racomitrium japonicum and their potential as biofertilizers for moss production

    Akio Tani, Motomu Akita, Haruhiko Murase, Kazuhide Kimbara

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   112 ( 1 )   32 - 39   2011年7月

     詳細を見る

    記述言語:英語   出版者・発行元:SOC BIOSCIENCE BIOENGINEERING JAPAN  

    The use of Racomitrium japonicum, a drought resistant bryophyte used for roof-greening, is gradually increasing. However, its utilization is hampered by slow growth rate. Here we isolated culturable bacteria from hydroponic cultivation samples to identify isolates that could promote moss growth. Most of the isolates belonged to Pseudomonas, Rhodococcus, and Duganella species. The isolates were biochemically characterized according to their type of interaction with plants, i.e., production of auxin, siderophores, or hydrogen cyanate, growth in the absence of an added nitrogen source, calcium phosphate solubilization, utilization of sugars, polymers, or aliphatic compounds, and antifungal activity. The isolates were applied to sterile protonemata and non-sterile adult gametophytes of R japonicum to evaluate their effect on plant growth. Furthermore, we isolated fungi that inhibited moss growth. Our results suggest that the microbial community structure in hydroponic cultures is important to stabilize moss production and the isolates that promote moss growth have potential to be utilized as biofertilizers for moss production. (C) 2011, The Society for Biotechnology, Japan. All rights reserved.

    DOI: 10.1016/j.jbiosc.2011.03.012

    Web of Science

    PubMed

    researchmap

  • 野菜葉圏に生息するMethylobacterium属細菌の分布

    水野雅之, 吉田奈央子, 谷明生, 由里本博也, 阪井康能

    日本農芸化学会大会講演要旨集   2011   258   2011年3月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • Characterization of a Cryptic Plasmid, pSM103mini, from Polyethylene-Glycol Degrading Sphingopyxis macrogoltabida Strain 103 国際誌

    Akio Tani, Akiyuki Tanaka, Toshiyuki Minami, Kazuhide Kimbara, Fusako Kawai

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   75 ( 2 )   295 - 298   2011年2月

     詳細を見る

    記述言語:英語   出版者・発行元:TAYLOR & FRANCIS LTD  

    A cryptic plasmid, pSM103mini, was found in polyethylene-glycol degrading bacterium Sphingopyxis macrogoltabida, strain 103. The plasmid was 4,328-bp long and its GC content was 57.5%. It contained four open reading frames, but only two of them showed significant similarity to reported proteins. ORF3 and ORF4 were assumed to encode resolvase and replication protein (RepA) respectively. Downstream of ORF4 we found complex repeat sequences. These together with 0121;3 and 4 were necessary and sufficient for plasmid maintenance in strain 103, as analyzed by constructing deletion plasmids. The pHSG398-fused plasmid (pHSG-SM103mini) functioned as a shuttle vector between strain 103 and Escherichia coli. The plasmid constructed was maintained in strain 103 and its close relative, S. macrogoltabida strain 203, but not efficiently in PEG-degrading S. terrae.

    DOI: 10.1271/bbb.100650

    Web of Science

    PubMed

    researchmap

  • Characterization of a Cryptic Plasmid, pSM103mini, from Polyethylene-Glycol Degrading Sphingopyxis macrogoltabida Strain 103

    Akio Tani, Akiyuki Tanaka, Toshiyuki Minami, Kazuhide Kimbara, Fusako Kawai

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   75 ( 2 )   295 - 298   2011年2月

     詳細を見る

    記述言語:英語   出版者・発行元:TAYLOR & FRANCIS LTD  

    A cryptic plasmid, pSM103mini, was found in polyethylene-glycol degrading bacterium Sphingopyxis macrogoltabida, strain 103. The plasmid was 4,328-bp long and its GC content was 57.5%. It contained four open reading frames, but only two of them showed significant similarity to reported proteins. ORF3 and ORF4 were assumed to encode resolvase and replication protein (RepA) respectively. Downstream of ORF4 we found complex repeat sequences. These together with 0121;3 and 4 were necessary and sufficient for plasmid maintenance in strain 103, as analyzed by constructing deletion plasmids. The pHSG398-fused plasmid (pHSG-SM103mini) functioned as a shuttle vector between strain 103 and Escherichia coli. The plasmid constructed was maintained in strain 103 and its close relative, S. macrogoltabida strain 203, but not efficiently in PEG-degrading S. terrae.

    DOI: 10.1271/bbb.100650

    Web of Science

    researchmap

  • Genes involved in novel adaptive aluminum resistance in Rhodotorula glutinis

    Akio Tani, Takaya Kawahara, Yoko Yamamoto, Kazuhide Kimbara, Fusako Kawai

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   109 ( 5 )   453 - 458   2010年5月

     詳細を見る

    記述言語:英語   出版者・発行元:SOC BIOSCIENCE BIOENGINEERING JAPAN  

    Rhodotorula glutinis IFO1125 acquired increased aluminum (Al) resistance from 50 mu M to more than 5 mM by repetitive culturing with stepwise increases in Al concentration at pH 4.0. In our previous study we performed differential display to find that three genes (RgFET3, RgGET3, and RgCMK) encoding proteins homologous to Saccharomyces cerevisiae FET3p, GET3p, and CMK1p and CMK2p, respectively, were up-regulated in the Al-resistant cells. In this study we cloned these genes and found they were indeed up-regulated in Al-resistant strains. The cloned genes were introduced into S. cerevisiae and corresponding mutants to test their relevance to Al resistance. The introduction of RgFET3 and RgGET3 conferred Al resistance to the host, but that of RgCMK did not. Green fluorescent protein (GFP)-tagged RgFet3p was localized at the cell periphery in the host. GFP-tagged RgGet3p formed more punctate bodies in the host under Al stress than in the absence of Al. Different growth responses to Fe (III), Cu (II), Ca ions, and cyclosporin A in the wild type and resistant cells of R. glutinis suggested the involvement and possible links of the three genes in Al resistance. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.

    DOI: 10.1016/j.jbiosc.2009.10.015

    Web of Science

    PubMed

    researchmap

  • IncP‐7群プラスミドの宿主域の解析

    松井一泰, 新谷政己, 神原将希, 谷明生, 金原和秀, 山根久和, 野尻秀昭

    日本農芸化学会大会講演要旨集   2010   193   2010年3月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • Flow cytometry and cell-sorting method for isolating live bacteria with meta-cleavage activity on dihydroxy compounds of biphenyl

    Sou Iijima, Yumi Shimomura, Akio Tani, Kazuhide Kimbara

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   108   S87 - S88   2009年11月

     詳細を見る

    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:SOC BIOSCIENCE BIOENGINEERING JAPAN  

    DOI: 10.1016/j.jbiosc.2009.08.257

    Web of Science

    researchmap

  • Probiotic potential of lactic acid bacteria isolated from chicken gastrointestinal digestive tract

    H. Musikasang, A. Tani, A. H-kittikun, S. Maneerat

    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY   25 ( 8 )   1337 - 1345   2009年8月

     詳細を見る

    記述言語:英語   出版者・発行元:SPRINGER  

    This study was conducted in order to evaluate the probiotic properties of lactic acid bacteria (LAB) isolated from intestinal tract of broilers and Thai indigenous chickens. The major properties, including the gastric juice and bile salts tolerance, starch, protein and lipid digesting capabilities, and the inhibition on certain pathogenic bacteria were investigated. Three-hundred and twenty-two and 226 LAB strains were isolated from ten broilers and eight Thai indigenous chickens, respectively. The gastrointestinal transit tolerance of these 548 isolates was determined by exposing washed cell suspension at 41A degrees C to simulated gastric juice (pH 2.5) containing pepsin (3 mg ml(-1)), and to simulated small intestinal juice (pH 8.0) in the presence of pancreatin (1 mg ml(-1)) and 7% fresh chicken bile, mimicking the gastrointestinal environment. The survival of 20 isolates was found after passing through the gastrointestinal conditions. The survival rates of six strains; KT3L20, KT2CR5, KT10L22, KT5S19, KT4S13 and PM1L12 from the sequential study were 43.68, 37.56, 33.84, 32.89, 31.37 and 27.19%, respectively. Twelve isolates exhibited protein digestion on agar plate but no isolates showed the ability to digest starch and lipid. All 20 LAB showed the antimicrobial activity against Salmonella sp., Staphylococcus aureus and Escherichia coli except one strain which did not show the inhibitory activity toward E. coli. Accordingly, five isolates of selected LAB (KT2L24, KT3L20, KT4S13, KT3CE27 and KT8S16) can be classified as the best probiotics and were identified as Enterococcus faecalis, Enterococcus durans, Enterococcus faecium, Pediococcus pentosaceus, and Enterococcus faecium, respectively. The survival rate of microencapsulation of E. durans KT3L20 under simulated small intestine juice after sequential of simulated gastric juice was also investigated. An extrusion technique exhibited a higher survival rate than emulsion technique and free cell, respectively.

    DOI: 10.1007/s11274-009-0020-8

    Web of Science

    researchmap

  • Biomarkers of green roof vegetation: anthocyanin and chlorophyll as stress marker pigments for plant stresses of roof environments

    I. C. Mori, S. Utsugi, S. Tanakamaru, A. Tani, T. Enomoto, M. Katsuhara

    J. Environ. Eng. Management   19   21 - 27   2009年

     詳細を見る

  • 岡山大学資源生物科学研究所における屋上緑化による建物冷却効果

    且原真木, 田中丸重美, 森泉, 谷明生, 宇都木繁子, 榎本敬, 米谷俊彦

    環境制御   31 ( 1 )   21 - 25   2009年

     詳細を見る

  • Involvement of PEG-carboxylate dehydrogenase and glutathione S-transferase in PEG metabolism by Sphingopyxis macrogoltabida strain 103 国際誌

    Peechapack Somyoonsap, Akio Tani, Jittima Charoenpanich, Toshiyuki Minami, Kazuhide Kimbara, Fusako Kawai

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   81 ( 3 )   473 - 484   2008年12月

     詳細を見る

    記述言語:英語   出版者・発行元:SPRINGER  

    Sphingopyxis terrae and the Sphingopyxis macrogoltabida strains 103 and 203 are able to degrade polyethylene glycol (PEG). They possess the peg operon, which is responsible for the conversion of PEG to PEG-carboxylate-coenzyme A (CoA). The upstream (3.0 kb) and downstream (6.5 kb) regions of the operon in strain 103 were cloned and sequenced. The structure was well conserved between S. macrogoltabida strain 203 and S. terrae, except that two sets of transposases are absent in strain 203. The downstream region contains the genes for PEG-carboxylate dehydrogenase (PCDH), glutathione S-transferase (GST), tautomerase, and a hypothetical protein. The genes for pcdh and gst were transcribed constitutively and monocistronically, indicating that their transcription is independent of the operon regulation. PCDH and GST were expressed in Escherichia coli and characterized biochemically. PCDH is a homotetramer of 64-kDa subunits and contains one molecule of flavin adenine dinucleotide per subunit. The enzyme dehydrogenates PEG-carboxylate to yield glyoxylate, suggesting that the enzyme is the third enzyme involved in PEG degradation. GST is a homodimer of 28-kDa subunits. GST activity was noncompetitively inhibited by acyl-CoA and PEG-carboxylate-CoA, suggesting the interaction of GST with them. The proposed role for GST is to buffer the toxicity of PEG-carboxylate-CoA.

    DOI: 10.1007/s00253-008-1635-7

    Web of Science

    PubMed

    researchmap

  • The crucial role of mitochondrial regulation in adaptive aluminium resistance in Rhodotorula glutinis

    Akio Tani, Chiemi Inoue, Yoko Tanaka, Yoko Yamamoto, Hideki Kondo, Syuntaro Hiradate, Kazuhide Kimbara, Fusako Kawai

    MICROBIOLOGY-SGM   154   3437 - 3446   2008年11月

     詳細を見る

    記述言語:英語   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    Rhodotorula glutinis IFO1125 was found to acquire increased aluminium (Al) resistance from 50 mu M to more than 5 mM by repetitive culturing with stepwise increases in Al concentration at pH 4.0. To investigate the mechanism underlying this novel phenomenon, wild-type and Al- resistant cells were compared. Neither cell type accumulated the free form of Al (Al3+) added to the medium. Transmission electron microscopic analyses revealed a greater number of mitochondria in resistant cells. The formation of small mitochondria with simplified cristae structures was observed in the wild-type strain grown in the presence of Al and in resistant cells grown in the absence of Al. Addition of Al to cells resulted in high mitochondrial membrane potential and concomitant generation of reactive oxygen species (ROS). Exposure to Al also resulted in elevated levels of oxidized proteins and oxidized lipids. Addition of the antioxidants a-tocopherol and ascorbic acid alleviated the Al toxicity, suggesting that ROS generation is the main cause of Al toxicity. Differential display analysis indicated upregulation of mitochondrial genes in the resistant cells. Resistant cells were found to have 2.5- to 3-fold more mitochondrial DNA (mtDNA) than the wild-type strain. Analysis of tricarboxylic acid cycle and respiratory-chain enzyme activities in wild-type and resistant cells revealed significantly reduced cytochrome c oxidase activity and resultant high ROS production in the latter cells. Taken together, these data suggest that the adaptive increased resistance to Al stress in resistant cells resulted from an increased number of mitochondria and increased mtDNA content, as a compensatory response to reduced respiratory activity caused by a deficiency in complex IV function.

    DOI: 10.1099/mic.0.2007/016048-0

    Web of Science

    researchmap

  • Proposed oxidative metabolic pathway for polypropylene glycol in Sphingobium sp strain PW-1

    Xiaoping Hu, Xin Liu, Akio Tani, Kazuhide Kimbara, Fusako Kawai

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   72 ( 4 )   1115 - 1118   2008年4月

     詳細を見る

    記述言語:英語   出版者・発行元:TAYLOR & FRANCIS LTD  

    Polypropylene glycol (PPG)-assimilating Sphingobium sp. strain PW-1 was grown on 0.5% PPG 700. PPG and its metabolites were analyzed by MALDI-TOF mass spectrometry. An oxidized form of a terminal alcohol group appeared with each molecular species as a metabolite. Cell-free extract showed PPG dehydrogenase activity. In this way, the oxidative metabolic pathway for PPG was confirmed.

    DOI: 10.1271/bbb.70749

    Web of Science

    researchmap

  • Polyethylene glycol (PEG)-carboxylate-CoA synthetase is involved in PEG metabolism in Sphingopyxis macrogoltabida strain 103 国際誌

    Akio Tani, Peechapack Somyoonsap, Toshiyuki Minami, Kazuhide Kimbara, Fusako Kawai

    ARCHIVES OF MICROBIOLOGY   189 ( 4 )   407 - 410   2008年4月

     詳細を見る

    記述言語:英語   出版者・発行元:SPRINGER  

    Sphingopyxis macrogoltabida strain 103 possesses polyethylene-glycol (PEG)-inducible pegBCDAE operon encoding the genes relevant to PEG degradation. PEG is converted to PEG-carboxylate by PegA (PEG dehydrogenase) and PegC (PEG-aldehyde dehydrogenase). In this study, the recombinant PegE (homologous to acyl-CoA synthetases) was characterized. PegE was an acyl-CoA synthetase active for PEG-carboxylate and fatty acids. Judging from the nature of this kind of protein (located on the cytoplasmic membrane as a translocator), PegE might be responsible for the translocation of PEG-carboxylate from the periplasm into the cytoplasm or for the detoxification of strong acidity of the substrate.

    DOI: 10.1007/s00203-007-0320-z

    Web of Science

    PubMed

    researchmap

  • The pva operon is located on the megaplasmid of Sphingopyxis sp strain 113P3 and is constitutively expressed, although expression is enhanced by PVA 国際誌

    Xiaoping Hu, Rie Mamoto, Yoshio Fujioka, Akio Tani, Kazuhide Kimbara, Fusako Kawai

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   78 ( 4 )   685 - 693   2008年3月

     詳細を見る

    記述言語:英語   出版者・発行元:SPRINGER  

    The upstream and downstream regions of the tentative pva operon including genes encoding oxidized polyvinyl alcohol (PVA) hydrolase (oph), PVA dehydrogenase (pvaA) and cytochrome c (cytC) from Sphingopyxis sp. strain 113P3 were sequenced. The resultant 7.9 kb sequence contained orf1 in the upstream region and orf2 and orf3 in the downstream region. Reverse transcription-polymerase chain reaction (PCR) analyses revealed that the intergenic regions between orf1 and oph or between cytC and orf2 were expressed neither in PVA medium nor glucose medium, indicating that the pva operon consists of three genes. A transcription start site was determined by 5'-rapid amplification of cDNA ends to be 428 bp upstream of the start codon of the oph. The stop codon of cytC was followed by a sequence of inverted repeats that could function as a factor-independent transcription terminator. Strain 113P3 had one megaplasmid including the pva operon. Northern blot hybridization for the three genes revealed that mRNA size was approximately 3 to 4 kb and expression was elevated in PVA medium compared to glucose medium.

    DOI: 10.1007/s00253-008-1348-y

    Web of Science

    PubMed

    researchmap

  • IncP‐7群カルバゾール分解プラスミドpCAR1の宿主域の解析

    松井一泰, 新谷政己, 谷明生, 金原和秀, 山根久和, 野尻秀昭

    環境バイオテクノロジー学会大会プログラム講演要旨集   34th   36   2008年

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • Structure and conservation of a polyethylene glycol-degradative operon in sphingomonads

    Akio Tani, Jittima Charoenpanich, Terumi Mori, Mayuko Takeichi, Kazuhide Kimbara, Fusako Kawai

    MICROBIOLOGY-SGM   153   338 - 346   2007年2月

     詳細を見る

    記述言語:英語   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    Sphingopyxis terrae, and Sphingopyxis macrogoltabida strains 103 and 203, can degrade polyethylene glycols (PEGs). They differ in the following respects: (i) different substrate specificities (chain length) of assimilable PEG, (ii) PEG-inducible or constitutive PEG-degradative proteins, and (iii) symbiotic or axenic degradation of PEG. S. terrae was able to incorporate PEG 6000, but strain 103 could not incorporate more than PEG 4000, suggesting that the difference in assimilable PEG chain length depends on the ability to take up substrate. PEG-degradative genes (pegB, C, D, A, E and R) from these strains were cloned. Their primary structures shared a high homology of more than 99%. The peg genes encode a TonB-dependent receptor (pegB), a PEG-aldehyde dehydrogenase (pegC), a permease (pegD), a PEG dehydrogenase (pegA) and an acyl-CoA ligase (pegE), and in the opposite orientation, an AraC-type transcription regulator (pegR). The peg operon was flanked by two different sets of transposases. These three strains contained large plasmids and the operon was located in one of the large plasmids in S. terrae. The peg genes could be detected in other PEG-degrading sphingomonads. These results suggest that the peg genes have evolved in a plasmid-mediated manner. An insertion of a transposon gene (pegF) between pegD and pegA in strain 203 was found, which caused the constitutive expression of pegA in this strain.

    DOI: 10.1099/mic.0.2006/000992-0

    Web of Science

    researchmap

  • Xenoestrogenic short ethoxy chain nonylphenol is oxidized by a flavoprotein alcohol dehydrogenase from Ensifer sp strain AS08 国際誌

    Xin Liu, Akio Tani, Kazuhide Kimbara, Fusako Kawai

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   73 ( 6 )   1414 - 1422   2007年1月

     詳細を見る

    記述言語:英語   出版者・発行元:SPRINGER  

    The ethoxy chains of short ethoxy chain nonylphenol (NPEOav2.0, containing average 2.0 ethoxy units) were dehydrogenated by cell-free extracts from Ensifer sp. strain AS08 grown on a basal medium supplemented with NPEOav2.0. The reaction was coupled with the reduction in 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide and phenazine methosulfate. The enzyme ( NPEOav2.0 dehydrogenase; NPEO-DH) was purified to homogeneity with a yield of 20% and a 56-fold increase in specific activity. The molecular mass of the native enzyme was 120 kDa, consisting of two identical monomer units ( 60 kDa). The gene encoding NPEO-DH was cloned, which consisted of 1,659 bp, corresponding to a protein of 553 amino acid residues. The deduced amino acid sequence agreed with the N-terminal amino acid sequence of the purified NPEO-DH. The presence of a flavin adenine dinucleotide (FAD)-binding motif and glucose-methanol-choline (GMC) oxidoreductase signature motifs strongly suggested that the enzyme belongs to the GMC oxidoreductase family. The protein exhibited homology (40-45% identity) with several polyethylene glycol dehydrogenases (PEG-DHs) of this family, but the identity was lower than those ( approximately 58%) among known PEG-DHs. The substrate- binding domain was more hydrophobic compared with those of glucose oxidase and PEG-DHs. The recombinant protein had the same molecular mass as the purified NPEO-DH and dehydrogenated PEG400-2000, NPEOav2.0 and its components, and NPEOav10, but only slight or no activity was found using diethylene glycol, triethylene glycol, and PEG200.

    DOI: 10.1007/s00253-006-0620-2

    Web of Science

    PubMed

    researchmap

  • Dual regulation of a polyethylene glycol degradative operon by AraC-type and GaIR-type regulators in Sphingopyxis macrogoltabida strain 103 国際誌

    Jittima Charoenpanich, Akio Tani, Naoko Moriwaki, Kazuhide Kimbara, Fusako Kawai

    MICROBIOLOGY-SGM   152 ( Pt 10 )   3025 - 3034   2006年10月

     詳細を見る

    記述言語:英語   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    The genes for polyethylene glycol (PEG) catabolism (pegB, C, D, A and E) in Sphingopyxis macrogoltabida strain 103 were shown to form a PEG-inducible operon. The pegR gene, encoding an AraC-type regulator in the downstream area of the operon, is transcribed in the reverse direction. The transcription start sites of the operon were mapped, and three putative a sigma(70)-type promoter sites were identified in the pegB, pegA and pegR promoters. A promoter activity assay showed that the pegB promoter was induced by PEG and oligomeric ethylene glycols, whereas the pegA and pegR promoters were induced by PEG. Deletion analysis of the pegB promoter indicated that the region containing the activator-binding motif of an AraC/XyIS-type regulator was required for transcription of the pegBCDAE operon. Gel retardation assays demonstrated the specific binding of PegR to the pegB promoter. Transcriptional fusion studies of pegR with pegA and pegB promoters suggested that PegR regulates the expression of the pegBCDAE operon positively through its binding to the pegB promoter, but PegR does not bind to the pegA promoter. Two specific binding proteins for the pegA promoter were purified and identified as a GaIR-type regulator and an H2A histone fragment (histone-like protein, HU). The binding motif of a GaIR/Lacl-type regulator was found in the pegA and pegR promoters. These results suggested the dual regulation of the pegBCDAE operon through the pegB promoter by an AraC-type regulator, PegR (PEG-independent), and through the pegA and pegR promoters by a GaIR/Lacl-type regulator together with HU (PEG-dependent).

    DOI: 10.1099/mic.0.29127-0

    Web of Science

    PubMed

    researchmap

  • Metabolic pathway of xenoestrogenic short ethoxy chain-nonylphenol to nonylphenol by aerobic bacteria, Ensifer sp strain AS08 and Pseudomonas sp strain AS90 国際誌

    Xin Liu, Akio Tani, Kazuhide Kimbara, Fusako Kawai

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   72 ( 3 )   552 - 559   2006年9月

     詳細を見る

    記述言語:英語   出版者・発行元:SPRINGER  

    Ensifer sp. strain AS08 and Pseudomonas sp. strain AS90 degrading short ethoxy (EO) chain-nonylphenol (NP) [NPEOav2.0 containing NP mono- similar to tetraethoxylates (NP1EO similar to NP4EO); average 2.0 EO units] were isolated by enrichment cultures. Both strains grew on NP but not on octyl- and nonylphenol polyethoxylates (NPEOs) (average 10 EO units). Growth and degradation of NPEOav2.0 was increased with increased concentrations of yeast extract (0.02-0.5%) in a culture medium. Culture supernatants of both strains grown on NPEOav2.0 were analyzed by high-performance liquid chromatography, showing degradation of NP4EO-NP1EO. The metabolites from nonylphenol diethoxylate (NP2EO) by resting cells of both strains were identified by gas chromatography-mass spectrometry as nonylphenoxyethoxyacetic acid, NP1EO, nonylphenoxyacetic acid (NP1EC), and NP, while those from NP1EO were identified as NP1EC and NP. Cell-free extracts from strain AS08 grown on NPEOav2.0 dehydrogenated NPEOs, NPEOav2.0, NP2EO, NP1EO, and PEG 400, but the extracts were inactive toward di- similar to tetraethylene glycol. Aldehydes were formed in the reaction mixture of each substrate with cell-free extracts. From these results, the aerobic metabolic pathway for short EO chain-NP is proposed: A terminal alcohol group of the EO chain is oxidized to a carboxylic acid via an aldehyde, and then one EO unit is removed. This process is repeated until NP is produced.

    DOI: 10.1007/s00253-005-0288-z

    Web of Science

    PubMed

    researchmap

  • Cloning and expression of the gene for periplasmic poly(vinyl alcohol) dehydrogenase from Sphingomonas sp strain 113P3, a novel-type quinohaemoprotein alcohol dehydrogenase

    Rie Hirota-Mamoto, Ryoko Nagai, Shinjiro Tachibana, Masaaki Yasuda, Akio Tani, Kazuhide Kimbara, Fusako Kawai

    MICROBIOLOGY-SGM   152   1941 - 1949   2006年7月

     詳細を見る

    記述言語:英語   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    A gene for periplasmic poly(vinyl alcohol) (PVA) dehydrogenase (PVADH) was cloned, based on the N-terminal amino acid sequence of the purified PVADH from Sphingomonas sp. 113P3 and the sequence of the gene for PVADH (pvaA, GenBank accession no. AB 190288). The recombinant PVADH tagged with hexahistidine was expressed in Escherichia coli and purified to homogeneity. The recombinant enzyme had the same characteristics as the purified enzyme from Sphingomonas sp. strain 113P. In addition to PVA, the recombinant PVADH could oxidize glycols such as polypropylene glycols and 1,3-butane/cyclohexanediol and 2,4-pentanediol, but neither primary nor secondary alcohols. The amino acid sequence of the recombinant PVADH showed similarity with those of PVADH from Pseudomonas sp. strain VM15C, putative PVADHs from Azoarcus sp. EbN1, and Xanthomonas species (54-25% identity), and the quinohaemoprotein alcohol dehydrogenases (OH-ADHs) from Comamonas testosteroni, Ralstonia eutropha and Pseudomonas putida (25-29% identity). PVADHs from strains 113P3 and VM15C have a conserved superbarrel domain (SD), probable PQQ-binding amino acids in the SID and a haem-binding domain (HBD) (they should be designated QH-PVADHs), but the positions of the amino acid sequences for the HBD and SID are the reverse of those of OH-ADHs. A protein structure of QH-PVADHs is proposed. Results of dot-blot hybridization and RT-PCR indicated that the three genes encoding oxidized PVA hydrolase, PVADH and cytochrome c are expressed constitutively and form an operon.

    DOI: 10.1099/mic.0.28848-0

    Web of Science

    researchmap

  • Analysis of amino acid residues involved in catalysis of polyethylene glycol dehydrogenase from Sphingopyxis terrae, using three-dimensional molecular modeling-based kinetic characterization of mutants

    T Ohta, T Kawabata, K Nishikawa, A Tani, K Kimbara, F Kawai

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   72 ( 6 )   4388 - 4396   2006年6月

     詳細を見る

    記述言語:英語   出版者・発行元:AMER SOC MICROBIOLOGY  

    Polyethylene glycol dehydrogenase (PEGDH) from Sphingopyxis terrae (formerly Sphingomonas terrae) is composed of 535 amino acid residues and one flavin adenine dinucleotide per monomer protein in a homodimeric structure. Its amino acid sequence shows 28.5 to 30.5% identity with glucose oxidases from Aspergillus niger and Penicillium amagasakiense. The ADP-binding site and the signature 1 and 2 consensus sequences of glucose-methanol-choline oxidoreductases are present in PEGDH. Based on three-dimensional molecular modeling and kinetic characterization of wild-type PEGDH and mutant PEGDHs constructed by site-directed mutagenesis, residues potentially involved in catalysis and substrate binding were found in the vicinity of the flavin ring. The catalytically important active sites were assigned to His-467 and Asn-511. One disulfide bridge between Cys-379 and Cys-382 existed in PEGDH and seemed to play roles in both substrate binding and electron mediation. The Cys-297 mutant showed decreased activity, suggesting the residue's importance in both substrate binding and electron mediation, as well as Cys-379 and Cys-382. PEGDH also contains a motif of a ubiquinone-binding site, and coenzyme Q(10) was utilized as an electron acceptor. Thus, we propose several important amino acid residues involved in the electron transfer pathway from the substrate to ubiquinone.

    DOI: 10.1128/AEM.02174-05

    Web of Science

    researchmap

  • A novel nicotinoprotein aldehyde dehydrogenase involved in polyethylene glycol degradation

    T Ohta, A Tani, K Kimbara, F Kawai

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   68 ( 5 )   639 - 646   2005年9月

     詳細を見る

    記述言語:英語   出版者・発行元:SPRINGER  

    A gene (pegC) encoding aldehyde dehydrogenase (ALDH) was located 3.4 kb upstream of a gene encoding polyethylene glycol (PEG) dehydrogenase (pegA) in Sphingomonas macrogoltabidus strain 103. ALDH was expressed in Escherichia coli and purified on a Ni-nitrilotriacetic acid agarose column. The recombinant enzyme was a homotetramer consisting of four 46.1-kDa subunits. The alignment of the putative amino acid sequence of the cloned enzyme showed high similarity with a group of NAD(P)-dependent ALDHs (identity 36-52%); NAD-binding domains (Rossmann fold and four glycine residues) and catalytic residues (Glu225 and Cys259) were well conserved. The cofactor, which was extracted from the purified enzyme, was tightly bound to the enzyme and identified as NADP. The enzyme contained 0.94 mol NADP per subunit. The enzyme was activated by Ca2+, but by no other metals; no metal (Zn, Fe, Mg, or Mn) was detected in the purified recombinant enzyme. Activity was inhibited by p-chloromercuric benzoate, and heavy metals such as Hg, Cu, Pb and Cd, indicating that a cysteine residue is involved in the activity. Enzyme activity was independent of N,N-dimethyl-p-nitrosoaniline as an electron acceptor. Trans-4-(N,N-dimethylamino)-cinnamaldehyde was not oxidized as a substrate, but the compound worked as an inhibitor for the enzyme, as did pyrazole. The enzyme acted on n-aldehydes C-2-C-14) and PEG-aldehydes. Thus the enzyme was concluded to be a novel Ca2+-activating nicotinoprotein (NADP-containing) PEG-aldehyde dehydrogenase involved in the degradation of PEG in S. macrogoltabidus strain 103.

    DOI: 10.1007/s00253-005-1936-z

    Web of Science

    researchmap

  • Determination of nonylphenol and short ethoxy chain nonylphenols on a normal phase silica-gel column high performance liquid chromatography

    L Xin, A Tani, F Kawai

    CHINESE JOURNAL OF ANALYTICAL CHEMISTRY   33 ( 8 )   1189 - 1191   2005年8月

     詳細を見る

    記述言語:中国語   出版者・発行元:ELSEVIER SCIENCE INC  

    High performance liquid chromatography( HPLC) on a normal phase silica-gel column with isocratic elution method was established to determine nonylphenol ( NP ) and short ethoxy chain nonylphenols ( NPnEO n = 1 similar to 3 ). NP and NPnEO were separated on a Cosmosil packed column 5SL- II (250 mm x 4. 6 mm i. d. 5 mu m) using ethyl acetate-ethanol as mobile phase at a flow rate of 1. 0 mL/min and detected at 281 run. Within 10 minutes all of the target compounds were eluted and separated. The limit of detection for NP and NPnEO ( n = 1 similar to 3 ) were 0. 1 mg/L and 0. 5 mg/L ( n = 1 similar to 3) respectively. The relative standard deviations of NP, NPEO, NP2EO and NP3EO were 1. 4%, 1. 6%, 2. 5% and 1. 4%, respectively.

    Web of Science

    researchmap

  • Regulation of genes relevant to microbial degradation of xenobiotic polymers.

    F Kawai, A Tani, K Kimbara

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY   229   U935 - U935   2005年3月

     詳細を見る

    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:AMER CHEMICAL SOC  

    Web of Science

    researchmap

  • Adaptive Al-resistance in Rhodotorula glutinis IFO1125

    Akio Tani, Takaya Kawahara, Yoko Yamamoto, Kazuhide Kimbara, Fusako Kawai

    Yeast   2005年

     詳細を見る

  • Purification, characterization and gene cloning of periplasmic oxidized polyvinyl alcohol hydrolase from Sphingomonas sp. strain 113P3

    Wilailak Klomklang, Akio Tani, Kazuhide Kimbara, Rie Mamoto, Takeshi Ueda, Masayuki Shimao, Fusako Kawai

    Microbiology   2005年

     詳細を見る

  • A new ether bond-splitting enzyme found in Gram-positive polyethylene glycol 6000-utilizing bacterium, Pseudonocardia sp strain K1 国際誌

    M Yamashita, A Tani, F Kawai

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   66 ( 2 )   174 - 179   2004年12月

     詳細を見る

    記述言語:英語   出版者・発行元:SPRINGER  

    Pseudonocardia sp. strain K1 is the only Gram-positive bacterium among the bacteria aerobically metabolizing polyethylene glycol (PEG). Generally, PEG is metabolized by an oxidative pathway in which a terminal alcohol group of PEG is oxidized to aldehyde and to carboxylic acid and then an ether bond is oxidatively cleaved. As the cell-free extract of Pseudonocardia sp. strain K1 has PEG dehydrogenase, PEG aldehyde dehydrogenase and diglycolic acid (DGA) dehydrogenase (DGADH) activities, all of which are constitutively formed, the strain has a metabolic pathway similar to that so far known. We purified an ether bond-splitting enzyme as DGADH. The molecular mass of the enzyme was estimated to be 55 kDa; and it consisted of two identical subunits. The enzyme oxidatively cleaved both an ether bond of PEG 3000 dicarboxylic acid and DGA. The N-terminal amino acid sequence of the purified enzyme had high homology with various superoxide dismutases and the enzyme had also superoxide dismutase activity. The atomic absorption spectrum showed that approximately one atom of Fe was included in each subunit of the enzyme. DGADH activity increased in the cells grown in a PEG medium supplemented with FeCl3. Thus, we concluded that the enzyme purified from Pseudonocardia sp. strain K1 is a new ether bond-splitting enzyme.

    DOI: 10.1007/s00253-004-1709-0

    Web of Science

    PubMed

    researchmap

  • Cloning and expression of an ether-bond-cleaving enzyme involved in the metabolism of polyethylene glycol

    M Yamashita, A Tani, F Kawai

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   98 ( 4 )   313 - 315   2004年10月

     詳細を見る

    記述言語:英語   出版者・発行元:SOC BIOSCIENCE BIOENGINEERING JAPAN  

    A gene encoding an ether-bond-cleaving enzyme, diglycolic acid dehydrogenase (DGADH) from polyethylene glycol-utilizing Pseudonocardia sp. strain K1, was cloned and expressed in Escherichia coli. The deduced amino acid sequence had a high homolog with superoxide dismutases (SODs) from various bacteria. The recombinant protein showed the same activities as those of DGADH front Pseudonocardia sp. strain K1, namely. SOD activity and ether-bond-cleaving activity.

    DOI: 10.1263/jbb.98.313

    Web of Science

    PubMed

    researchmap

  • Treatment of the yeast Rhodotorula glutinis with AlCl3 leads to adaptive acquirement of heritable aluminum resistance

    A Tani, D Zhang, JA Duine, F Kawai

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   65 ( 3 )   344 - 348   2004年8月

     詳細を見る

    記述言語:英語   出版者・発行元:SPRINGER  

    When aluminum (Al) was added to a culture, growth of Rhodotorula glutinis IFO1125 was temporarily arrested, showing longer lag phases, depending on the Al concentrations (50-300 muM) added, but the growth rates were not affected at all. Resistant strains obtained by one round of plate treatment containing Al reverted the resistance level to the wild-type level when cultivated without Al. Repeated Al treatments, however, induced heritable and stable Al resistance, the level of which was increased up to 4,000 muM by stepwise increments in Al concentrations. Thus, the heritable Al resistance adaptively acquired was due neither to adaptation nor to mutation, but to a mechanism which has yet to be studied. Heritable Al resistance seemed to release the Al inhibition of magnesium uptake.

    DOI: 10.1007/s00253-003-1546-6

    Web of Science

    researchmap

  • Wax ester production by bacteria 国際誌

    T Ishige, A Tani, YR Sakai, N Kato

    CURRENT OPINION IN MICROBIOLOGY   6 ( 3 )   244 - 250   2003年6月

     詳細を見る

    記述言語:英語   出版者・発行元:CURRENT BIOLOGY LTD  

    The enzymological and genetic aspects of microbial metabolism of hydrocarbons have been extensively revealed. Such molecular information is useful for understanding the bioremediation of oil spill environments and production of hydrocarbon-specific fine chemicals.

    DOI: 10.1016/S1369-5274(03)00053-5

    Web of Science

    PubMed

    researchmap

  • Two acyl-CoA dehydrogenases of Acinetobacter sp strain M-1 that uses very long-chain n-alkanes

    A Tani, T Ishige, Y Sakai, N Kato

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   94 ( 4 )   326 - 329   2002年10月

     詳細を見る

    記述言語:英語   出版者・発行元:SOC BIOSCIENCE BIOENGINEERING JAPAN  

    Two genes encoding acyl-CoA dehydrogenases, acdA and acdB, arranged in tandem, were found in the chromosomal DNA of Acinetobacter sp. strain M-1. AcdA was purified from the parental strain and AcdB was purified from an Escherichia coli strain expressing the cloned gene. The substrate specificities of the two enzymes suggest that AcdA is a medium-chain acyl-CoA dehydrogenase and that AcdB is a long-chain acyl-CoA dehydrogenase. Characterization of n-alkane metabolism in Acinetobacter sp. strain M-1 has revealed parallel pathways as well as enzymes with overlapping specificities in a single pathway. The two acyl-CoA dehydrogenases described here provide another example of the physiological complexity underlying n-alkane utilization.

    DOI: 10.1263/jbb.94.326

    Web of Science

    PubMed

    researchmap

  • Wax ester production from n-alkanes by Acinetobacter sp strain M-1: Ultrastructure of cellular inclusions and role of acyl coenzyme A reductase 国際誌

    T Ishige, A Tani, K Takabe, K Kawasaki, Y Sakai, N Kato

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   68 ( 3 )   1192 - 1195   2002年3月

     詳細を見る

    記述言語:英語   出版者・発行元:AMER SOC MICROBIOLOGY  

    Acinetobacter sp. strain M-1 accumulated a large amount of wax esters from an n-alkane under nitrogen-limiting conditions. Under the optimized conditions with n-hexadecane as the substrate, the amount of hexadecyl hexadecanoate in the cells reached 0.17 g/g of cells (dry weight). Electron microscopic analysis revealed that multilayered disk-shaped intracellular inclusions were formed concomitant with wax ester formation. The contribution of acyl-CoA reductase to wax ester synthesis was evaluated by gene disruption analysis.

    DOI: 10.1128/AEM.68.3.1192-1195.2002

    Web of Science

    PubMed

    researchmap

  • トランスポゾン変異法によるMycobacterium sp. P101株のiso‐アルカン代謝に特異的な遺伝子の探索

    阪井康能, 高橋悠典, 石毛たける, 谷明生, 宮地信也, 加藤暢夫

    日本農芸化学会関西支部講演会講演要旨集   423rd   6   2002年2月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • iso‐アルカン分解菌の探索と分解菌の系統分類

    阪井康能, 高橋悠典, 石毛たける, 谷明生, 宮地伸也, 加藤暢夫

    日本生物工学会大会講演要旨集   2001   123   2001年9月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • 長鎖n‐アルカン資化性細菌Acinetobacter sp. M‐1株に見いだしたacyl‐CoA Reductaseの諸性質

    石毛たける, 谷明生, 阪井康能, 加藤暢夫

    日本農芸化学会誌   75 ( 4 )   521   2001年4月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • ガス状短鎖アルカン資化性細菌Gordonia sp.TY‐5株の分離とプロパン代謝

    石毛たける, 山本温子, 谷明生, 阪井康能, 加藤暢夫

    日本農芸化学会誌   75   219   2001年3月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • 長鎖n‐アルカン資化性細胞Acinetobacter sp.M‐1株に見いだした長鎖acyl‐CoA reductaseの生理的役割

    石毛たける, 谷明生, 阪井康能, 加藤暢夫

    日本農芸化学会誌   75   208   2001年3月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • Gene structures and regulation of the alkane hydroxylase complex in Acinetobacter sp strain M-1

    A Tani, T Ishige, Y Sakai, N Kato

    JOURNAL OF BACTERIOLOGY   183 ( 5 )   1819 - 1823   2001年3月

     詳細を見る

    記述言語:英語   出版者・発行元:AMER SOC MICROBIOLOGY  

    In the long-chain n-alkane degrader Acinetobacter sp. strain M-1, two alkane hydroxylase complexes are switched by controlling the expression of two n-alkane hydroxylase-encoding genes in response to the chain length of n-alkanes, while rubredoxin and rubredoxin ruductase are encoded by a single gene and expressed constitutively.

    DOI: 10.1128/JB.183.5.1819-1823.2001

    Web of Science

    researchmap

  • 環境修復や物質生産に利用できるAcinetobacter 属細菌の多様な代謝能

    谷 明生, 石毛たける, 阪井康能, 加藤暢夫

    バイオサイエンスとインダストリー   59(9), 599-604   2001年

     詳細を見る

  • Thermostable NADP(+)-dependent medium-chain alcohol dehydrogenase from Acinetobacter sp strain M-1: Purification and characterization and gene expression in Escherichia coli

    A Tani, Y Sakai, T Ishige, N Kato

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   66 ( 12 )   5231 - 5235   2000年12月

     詳細を見る

    記述言語:英語   出版者・発行元:AMER SOC MICROBIOLOGY  

    NADPH-dependent alkylaldehyde reducing enzyme, which was greatly induced by n-hexadecane, from Acinetobacter sp, strain M-l was purified and characterized. The purified enzyme had molecular masses of 40 kDa as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 160 kDa as determined by gel filtration chromatography. The enzyme, which was shown to be highly thermostable, was most active toward n-heptanal and could use n-alkylaldehydes ranging from C-2 to C-14 and several substituted benzaldehydes, including the industrially important compounds cinnamyl aldehyde and anisaldehyde, as substrates. The alrA gene coding for this enzyme was cloned, and its nucleotide sequence was determined. The deduced amino acid sequence encoded by the alrA gene exhibited homology to the amino acid sequences of zinc-containing alcohol dehydrogenases from various sources. The gene could be highly expressed in Escherichia coli, and the product was purified to homogeneity by simpler procedures from the recombinant than from the original host. Our results show that this enzyme can be used for industrial bioconversion of useful alcohols and aldehydes.

    DOI: 10.1128/AEM.66.12.5231-5235.2000

    Web of Science

    researchmap

  • Thermostable NADP(+)-dependent medium-chain alcohol dehydrogenase from Acinetobacter sp strain M-1: Purification and characterization and gene expression in Escherichia coli

    A Tani, Y Sakai, T Ishige, N Kato

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   66 ( 12 )   5231 - 5235   2000年12月

     詳細を見る

    記述言語:英語   出版者・発行元:AMER SOC MICROBIOLOGY  

    NADPH-dependent alkylaldehyde reducing enzyme, which was greatly induced by n-hexadecane, from Acinetobacter sp, strain M-l was purified and characterized. The purified enzyme had molecular masses of 40 kDa as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 160 kDa as determined by gel filtration chromatography. The enzyme, which was shown to be highly thermostable, was most active toward n-heptanal and could use n-alkylaldehydes ranging from C-2 to C-14 and several substituted benzaldehydes, including the industrially important compounds cinnamyl aldehyde and anisaldehyde, as substrates. The alrA gene coding for this enzyme was cloned, and its nucleotide sequence was determined. The deduced amino acid sequence encoded by the alrA gene exhibited homology to the amino acid sequences of zinc-containing alcohol dehydrogenases from various sources. The gene could be highly expressed in Escherichia coli, and the product was purified to homogeneity by simpler procedures from the recombinant than from the original host. Our results show that this enzyme can be used for industrial bioconversion of useful alcohols and aldehydes.

    DOI: 10.1128/AEM.66.12.5231-5235.2000

    Web of Science

    researchmap

  • 長鎖n‐アルカン資化性細菌Acinetobacter sp.M‐1株に見いだしたacyl‐CoA reductaseの諸性質

    石毛たける, 谷明生, 阪井康能, 加藤暢夫

    日本農芸化学会関西支部講演会講演要旨集   416th   27   2000年10月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • Long-chain aldehyde dehydrogenase that participates in n-alkane utilization and wax ester synthesis in Acinetobacter sp strain M-1

    T Ishige, A Tani, Y Sakai, N Kato

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   66 ( 8 )   3481 - 3486   2000年8月

     詳細を見る

    記述言語:英語   出版者・発行元:AMER SOC MICROBIOLOGY  

    A long-chain aldehyde dehydrogenase, Ald1, was found in a soluble fraction of Acinetobacter sp, strain M-1 cells grown on n-hexadecane as a sole carbon source, The gene (ald1) was cloned from the chromosomal DNA of the bacterium. The open reading frame of ald1 was 1,512 bp long, cor:responding to a protein of 503 amino acid residues (molecular mass, 55,496 Da), and the deduced amino acid sequence showed high similarity to those of various aldehyde dehydrogenases, The ald1 gene was stably expressed in Escherichia coli, and the gene product (recombinant Ald1 [rAld1]) was purified to apparent homogeneity by gel electrophoresis, rAld1 showed enzyme activity toward n-alkanals (C-4 to C-14), with a preference, for longer carbon chains within the tested range; the highest activity was obtained with tetradecanal, The ald1 gene was disrupted by homologous recombination on the Acinetobacter genome. Although the ald1 disruptant (ald1 Delta) strain still had the ability to grow on n-hexadecane to some extent, its aldehyde dehydrogenase activity toward n-tetradecanal was reduced to half the level of the wild-type strain. Under nitrogen-limiting conditions, the accumulation of intracellular wax esters in the ald1 Delta strain became much lower than that in the wild-type strain. These and other results imply that a soluble long chain aldehyde dehydrogenase indeed plays important roles both in growth on n-alkane and in wax ester formation in Acinetobacter sp, strain M-1.

    DOI: 10.1128/AEM.66.8.3481-3486.2000

    Web of Science

    researchmap

  • 長鎖n‐アルカン分解菌Acinetobacter sp.M‐1由来の2つのアルカンヒドロキシラーゼの諸性質

    谷明生, 石毛たける, 阪井康能, 加藤暢夫

    日本生物工学会大会講演要旨集   2000   71   2000年7月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • アルカン資化性細菌Acinetobacter sp.M‐1由来acyl‐CoA reductaseのクローニング

    石毛たける, 谷明生, 阪井康能, 加藤暢夫

    日本生物工学会大会講演要旨集   2000   71   2000年7月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • Long-chain aldehyde dehydrogenase that participates in n-alkane utilization and wax ester synthesis in Acinetobacter sp. strain M-1

    T. Ishige, A. Tani, Y. Sakai, N. Kato

    Applied and Environmental Microbiology   66 ( 8 )   3481 - 3486   2000年

     詳細を見る

    記述言語:英語  

    A long-chain aldehyde dehydrogenase, Ald1, was found in a soluble fraction of Acinetobacter sp. strain M-1 cells grown on n-hexadecane as a sole carbon source. The gene (ald1) was cloned from the chromosomal DNA of the bacterium. The open reading frame of ald1 was 1,512 bp long, corresponding to a protein of 503 amino acid residues (molecular mass, 55,496 Da), and the deduced amino acid sequence showed high similarity to those of various aldehyde dehydrogenases. The ald1 gene was stably expressed in Escherichia coli, and the gene product (recombinant Ald1 [rAld1]) was purified to apparent homogeneity by gel electrophoresis. rAld1 showed enzyme activity toward n-alkanals (C4 to C14), with a preference for longer carbon chains within the tested range
    the highest activity was obtained with tetradecanal. The ald1 gene was disrupted by homologous recombination on the Acinetobacter genome. Although the ald1 disruptant (ald1Δ) strain still had the ability to grow on n-hexadecane to some extent, its aldehyde dehydrogenase activity toward n-tetradecanal was reduced to half the level of the wild-type strain. Under nitrogen-limiting conditions, the accumulation of intracellular wax esters in the ald1Δ strain became much lower than that in the wild-type strain. These and other results imply that a soluble long-chain aldehyde dehydrogenase indeed plays important roles both in growth on n-alkane and in wax ester formation in Acinetobacter sp. strain M-1.

    DOI: 10.1128/AEM.66.8.3481-3486.2000

    Scopus

    PubMed

    researchmap

  • Thermostable NADP+-dependent medium-chain alcohol dehydrogenase from Acinetobacter sp. Strain M-1: Purification and characterization and gene expression in Escherichia coli

    A. Tani, Y. Sakai, T. Ishige, N. Kato

    Applied and Environmental Microbiology   66 ( 12 )   5231 - 5235   2000年

     詳細を見る

    記述言語:英語  

    NADPH-dependent alkylaldehyde reducing enzyme, which was greatly induced by n-hexadecane, from Acinetobacter sp. strain M-1 was purified and characterized. The purified enzyme had molecular masses of 40 kDa as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 160 kDa as determined by gel filtration chromatography. The enzyme, which was shown to be highly thermostable, was most active toward n-heptanal and could use n-alkylaldehydes ranging from C2 to C14 and several substituted benzaldehydes, including the industrially important compounds cinnamyl aldehyde and anisaldehyde, as substrates. The alrA gene coding for this enzyme was cloned, and its nucleotide sequence was determined. The deduced amino acid sequence encoded by the alrA gene exhibited homology to the amino acid sequences of zinc-containing alcohol dehydrogenases from various sources. The gene could be highly expressed in Escherichia coli, and the product was purified to homogeneity by simpler procedures from the recombinant than from the original host. Our results show that this enzyme can be used for industrial bioconversion of useful alcohols and aldehydes.

    DOI: 10.1128/AEM.66.12.5231-5235.2000

    Scopus

    PubMed

    researchmap

  • Acinetobacter sp.M‐1由来の2つのアルカンヒドロキシラーゼのクローニング

    谷明生, 石毛たける, 阪井康能, 加藤暢夫

    日本生物工学会大会講演要旨集   1999   189   1999年8月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • アルカン資化性細菌Acinetobacter sp.M‐1に見出した長鎖アルデヒドデヒドロゲナーゼの生理的役割

    石毛たける, 阪井康能, 谷明生, 加藤暢夫

    日本生物工学会大会講演要旨集   1999   60   1999年8月

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • Acinetobacter sp.M‐1株由来長鎖n‐アルキルアルデヒドレダクターゼ遺伝子のクローニングとその発現

    阪井康能, 谷明生, 石毛たける, 加藤暢夫

    日本生物工学会大会講演要旨集   1997   151   1997年

     詳細を見る

    記述言語:日本語  

    J-GLOBAL

    researchmap

  • 長鎖n-アルカン資化性菌Acinetobacter sp. M-1のアルキルアルデヒドレダクターゼの精製と諸性質 : 環境化学

    孟 俊鎬, 谷 明生, 阪井 康能, 加藤 暢夫

    日本農藝化學會誌   70 ( 0 )   1996年3月

     詳細を見る

    記述言語:日本語   出版者・発行元:社団法人日本農芸化学会  

    CiNii Article

    researchmap

  • A non-conventional dissimilation pathway for long chain n-alkanes in Acinetobacter sp. M-1 that starts with a dioxygenase reaction

    Yasuyoshi Sakai, Jun Ho Maeng, Seigo Kubota, Akio Tani, Yoshiki Tani, Nobuo Kato

    Journal of Fermentation and Bioengineering   81 ( 4 )   286 - 291   1996年

     詳細を見る

    記述言語:英語   出版者・発行元:Society of Fermentation and Bioengineering, Japan  

    n-Alkane oxidation in a long chain n-alkane utilizer, Acinetobacter sp. M- l, was investigated. In Acinetobacter, n-alkanes have been postulated to be converted to the acid via a non-conventional oxidation pathway: n-alkane → n-alkyl hydroperoxide → aldehyde → acid (Finnerty, W.R.: Lipids of Acinetobacter. In Proceedings of the World Conference on Biotechnology for the Fats and Oils Industry, 184-188, 1988). However, there is little biochemical information on the enzymes involved in the postulated pathway, particularly the enzyme catalyzing the first step. In this study, we purified an n-alkane-oxidizing enzyme to apparent homogeneity by SDS-PAGE. The enzyme was a flavoprotein, and required molecular oxygen and Cu2+ for its activity, but did not require a reduced coenzyme such as NAD(P)H. A hydroperoxide was detected as a product of the enzyme reaction. We assume that the n-alkane-oxidizing enzyme is a dioxygenase. In addition, as a fatty alcohol does not appear to be an intermediate, fatty alcohol dehydrogenase is assumed not to participate in the n-alkane oxidation. The validity of the postulated pathway is supported by the following observations: (i) n-alkane monooxygenase activity was not detected, (ii) fatty alcohol dehydrogenase activities were low, and (iii) NAD(P)H-dependent long chain fatty aldehyde dehydrogenase activities were strongly induced in n-alkane-grown cells. NAD(P)H-dependent fatty aldehyde reductase activity was also found in n- alkane-grown cells, which may contribute to the formation of waxes that are cell reserve substances in n-alkane-utilizing Acinetobacter.

    DOI: 10.1016/0922-338X(96)80578-2

    Scopus

    researchmap

▼全件表示

講演・口頭発表等

  • 低栄養下におけるMethylorubrum属細菌のランタノイドによる生育促進応答に関する研究

    根本侑知、水野洸介、原田雄斗、岩本悟志、谷明生、三井亮司、島田昌也、早川享志、中川智行

    日本生物工学会大会  2021年10月27日 

     詳細を見る

    開催年月日: 2021年10月27日 - 2021年10月29日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:沖縄  

    researchmap

  • Genomic insights into the PPFMs phylogeny and unique features 国際共著

    Ola Alessa、 Yoshitoshi Ogura、 Yoshiko Fujitani、 Hideto Takami、 Tetsuya Hayashi、 Nurettin Sahin、 Akio Tani

    日本農芸化学会西日本・中四国・関西支部合同大会(第60回講演会)  2021年9月24日 

     詳細を見る

    開催年月日: 2021年9月24日 - 2021年9月25日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:鹿児島  

    researchmap

  • グラム陽性メチロトローフ細菌Arthrobacter sp. YM1の新規ランタノイド依存型メタノールデヒドロゲナーゼの精製と諸性質

    森田翔太、 任家宜、 谷明生、 中川智行、 阿野嘉孝、 矢野嵩典、 三井亮司

    日本農芸化学会西日本・中四国・関西支部合同大会(第60回講演会)  2021年9月24日 

     詳細を見る

    開催年月日: 2021年9月24日 - 2021年9月25日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:鹿児島  

    researchmap

  • オオムギ根から分離された新種細菌Rugamonas sp.の性質

    木代勝元、 最相大輔、 山下純、 山地直樹、 山本敏央、 門田有希、 持田恵一、 中川智行、 谷明生

    日本農芸化学会西日本・中四国・関西支部合同大会(第60回講演会)  2021年9月24日 

     詳細を見る

    開催年月日: 2021年9月24日 - 2021年9月25日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:鹿児島  

    researchmap

  • メチロバクテリウム属細菌におけるメタノール走化性の分子メカニズムの解明

    菊池志保、 藤谷良子、加藤純一 谷明生

    日本農芸化学会西日本・中四国・関西支部合同大会(第60回講演会)  2021年9月24日 

     詳細を見る

    開催年月日: 2021年9月24日 - 2021年9月25日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:鹿児島  

    researchmap

  • Isolation and identification of novel barley endophytes. 国際会議

    Kishiro K.、 Saisho D、 Yamashita J.、 Yamaji N.、 Yamamoto T.、 Monden Y.、 Mochida K.、 Nakagawa T.、 Tani A.

    Phyllosphere Fortnight 2021  2021年7月16日 

     詳細を見る

    開催年月日: 2021年7月16日 - 2021年7月21日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:California  

    researchmap

  • Comparative genomics and phenotyping approach on Methylobacterium species. 国際会議

    Alessa O.、 Ogura Y.、 Fujitani Y.、 Hayashi T.、 Sahin N.、 Tani A.

    Phyllosphere Fortnight 2021  2021年7月16日 

     詳細を見る

    開催年月日: 2021年7月16日 - 2021年7月21日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:California  

    researchmap

  • Functional analysis of siderophore in Methylobacterium aquaticum 22A. 国際会議

    Patrick Juma、 Ola Alessa、 Yoshiko Fujitani、 Tokitaka Oyama、 Hiroya Yurimoto、 Yasuyoshi Sakai、 Akio Tani

    Phyllosphere Fortnight 2021  2021年7月16日 

     詳細を見る

    開催年月日: 2021年7月16日 - 2021年7月21日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:California  

    researchmap

  • Comparative genomic analysis of the pink pigmented facultative methylotrophs (PPFMs) 国際会議

    Ola Alessa、 Yoshitoshi Ogura、 Yoshiko Fujitani、 Hideto Takami、 Tetsuya Hayashi、 Akio Tani

    Galaxy online meeting  2021年7月6日 

     詳細を見る

    開催年月日: 2021年7月6日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Belgium  

    researchmap

  • 植物表層共生細菌の生態と応用 招待

    谷 明生

    第2回生物刺激制御研究会  2021年6月11日 

     詳細を見る

    開催年月日: 2021年6月11日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:岡山  

    researchmap

  • 蛍光タンパク質を発現させた Methylobacterium 属細菌のアカシソ上での動態解析

    片山志織、由里本博也、谷明生、阪井康能

    日本農芸化学会大会  2021年3月18日 

     詳細を見る

    開催年月日: 2021年3月18日 - 2021年3月21日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:仙台  

    researchmap

  • Genetic and functional analysis of siderophore in Methylobacterium aquaticum 22A

    Patrick Juma、 Ola Alessa、 Yoshiko Fujitani、 Tokitaka Oyama、 Hiroya Yurimoto、 Yasuyoshi Sakai、 Akio Tani

    日本農芸化学会大会  2021年3月18日 

     詳細を見る

    開催年月日: 2021年3月18日 - 2021年3月21日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:仙台  

    researchmap

  • オオムギ根圏の共生微生物の単離と同定

    木代勝元、最相大輔、山下純、山地直樹、山本敏夫、門田有希、持田恵一、中川智行、谷 明生

    日本農芸化学会大会  2021年3月18日 

     詳細を見る

    開催年月日: 2021年3月18日 - 2021年3月21日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:仙台  

    researchmap

  • Comparative genomic analysis of the pink pigmented facultative methylotrophs (PPFM) 国際共著

    Ola Alessa、 Nurettin Sahin、 Yoshihisa Ogura、 Tetsuya Hayashi、 Akio Tani

    日本農芸化学会大会  2021年3月18日 

     詳細を見る

    開催年月日: 2021年3月18日 - 2021年3月21日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:仙台  

    researchmap

  • Methylobacterium 属細菌におけるメタノール走化性の分子メカニズムの解明

    菊池志保、藤谷良子、加藤純一、谷 明生

    日本農芸化学会大会  2021年3月18日 

     詳細を見る

    開催年月日: 2021年3月18日 - 2021年3月21日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:仙台  

    researchmap

  • Bacteria with natural chemotaxis towards methanol revealed by chemotaxis fishing technique

    谷 明生

    日本農芸化学会 中四国支部 第58回講演会(例会)  2021年1月23日 

     詳細を見る

    開催年月日: 2021年1月23日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:高知  

    researchmap

  • Methylobacterium 属細菌におけるメタノール走化性の分子メカニズムの解明

    菊池志保、谷 明生

    岡山バイオアクティブ研究会第57回シンポジウム  2020年10月21日 

     詳細を見る

    開催年月日: 2020年10月21日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:岡山  

    researchmap

  • オオムギの根圏に生息する微生物の調査

    木代 勝元、谷 明生

    岡山バイオアクティブ研究会第57回シンポジウム  2020年10月21日 

     詳細を見る

    開催年月日: 2020年10月21日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:岡山  

    researchmap

  • 植物葉上共生細菌Methylorubrum extorquens AM1のランタノイド依存型メタノールデヒドロゲナーゼアイソザイムXoxF2の解析

    竹内赴登、山下颯太、谷 明生、中川智行、矢野嵩典、三井亮司

    農芸化学会中四国支部講演会  2020年9月17日 

     詳細を見る

    開催年月日: 2020年9月17日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:愛媛大学  

    researchmap

  • Methylobacterium aquaticum 22A株における Lanmodulinの機能解析

    藤谷良子、谷 明生

    農芸化学会  2020年3月25日 

     詳細を見る

    開催年月日: 2020年3月25日 - 2020年3月27日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:博多  

    researchmap

  • Lanthanide-dependent methylotrophic pathway in Methylobacterium aquaticum strain 22A

    Patcha Yanpirat、 Akio Tani

    農芸化学会  2020年3月25日 

     詳細を見る

    開催年月日: 2020年3月25日 - 2020年3月27日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:博多  

    researchmap

  • Methylorubrum属細菌の低栄養環境下におけるランタノイド応答の分子メカニズムの解明

    水野洸介、原田雄斗、岩本悟志、谷明生、三井亮司、島田昌也、早川享志、中川智行

    農芸化学会  2020年3月25日 

     詳細を見る

    開催年月日: 2020年3月25日 - 2020年3月27日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:博多  

    researchmap

  • Methylobacterium aquaticum strain 22Aにおけるランタノイドスイッチのメカニズム

    宮本稚子、谷 明生

    農芸化学会  2020年3月25日 

     詳細を見る

    開催年月日: 2020年3月25日 - 2020年3月27日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:博多  

    researchmap

  • 植物葉上共生細菌Methylorubrum extorquens AM1のランタノイド依存型メタノールデヒドロゲナーゼアイソザイムのレポーター遺伝子を用いた発現解析

    山下颯太、吉川友理、 谷 明生、中川智行、矢野嵩典、三井亮司

    農芸化学会中四国支部講演会  2020年1月25日 

     詳細を見る

    開催年月日: 2020年1月25日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:愛媛大学  

    researchmap

  • Methylobacterium aquaticum 22A株におけるランタノイドスイッチのメカニズム

    宮本稚子、藤谷良子、谷 明生

    おかやまバイオアクティブ研究会  2019年10月8日 

     詳細を見る

    開催年月日: 2019年10月8日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:岡山理科大学  

    researchmap

  • ホウレンソウのポストハーベストにおける菌叢変遷と鮮度劣化へのPPFMsの関与

    Lun Wang、 繁原 安美、 阪口 由佳、谷 明生、中野浩平、島田雅也、早川享志、中川智行

    第71回日本生物工学会大会  2019年9月16日 

     詳細を見る

    開催年月日: 2019年9月16日 - 2019年9月18日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:岡山大学  

    researchmap

  • Methylobacterium aquaticum 22A株におけるランタノイドスイッチのメカニズム

    宮本稚子、谷明生

    日本農芸化学会中四国支部 第54回講演会(例会)  2019年6月1日 

     詳細を見る

    開催年月日: 2019年6月1日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:岡山理科大学  

    researchmap

  • Lanthanide-dependent methylotrophic pathways in Methylobacterium aquaticum 22A 国際会議

    Patcha Yanpirat and Akio Tani

    ASME and TSME Meeting 2019  2019年5月11日 

     詳細を見る

    開催年月日: 2019年5月11日 - 2019年5月13日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Taichung, Taiwan. Thunghai Univ.  

    researchmap

  • メチロトロフのランタノイド依存性研究の最前線 招待

    谷 明生

    日本農芸化学会大会  2019年3月24日 

     詳細を見る

    開催年月日: 2019年3月24日 - 2019年3月27日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京  

    researchmap

  • Lanthanide-dependent formaldehyde oxidation pathways in Methylobacterium aquaticum strain 22A

    Patcha yanpirat、 谷 明生

    日本農芸化学会大会  2019年3月24日 

     詳細を見る

    開催年月日: 2019年3月24日 - 2019年3月27日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:東京  

    researchmap

  • Methylobacterium aquaticum strain 22Aにおけるランタノイドスイッチのメカニズム

    宮本稚子、谷 明生

    日本農芸化学会大会  2019年3月24日 

     詳細を見る

    開催年月日: 2019年3月24日 - 2019年3月27日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京  

    researchmap

  • Methylobacterium aquaticum22A株におけるメチロタキシスの機能解析

    春名優希、加藤純一、谷 明生

    日本農芸化学会大会  2019年3月24日 

     詳細を見る

    開催年月日: 2019年3月24日 - 2019年3月27日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京  

    researchmap

  • Methylobacterium aquaticum 22A株におけるLa誘導性タンパクの機能解析

    藤谷良子、谷 明生

    日本農芸化学会大会  2019年3月24日 

     詳細を見る

    開催年月日: 2019年3月24日 - 2019年3月27日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:東京  

    researchmap

  • Exploration of marine bacteria with the potential for biological reduction of hexavalent chromium 国際会議

    Jittima Charoepanich、 Jutamas Pantab、 Jariya Supakit、 Jeerapan Saengkla、 Srisuda Nithettham、 Dang Saebea and Akio Tani

    Final Joint Seminar of CCP  2018年12月2日 

     詳細を見る

    開催年月日: 2018年12月2日 - 2018年12月4日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Yamaguchi University  

    researchmap

  • Characterization of thermotolerant ethanologenic yeasts isolated in Lao 81 PDR 国際会議

    Chansom Keo-oudone、 Sukanya Nitiyon、 Phonepasith Sotitham、 Mochamad Nurcholis、 Khonesavanh Milavong、 May Thammany、 Bouachanh Seeyakeo、 Maniphone Phoudphong、 Hiem Seulo、 Khamlar Sisouvong、 Vannapha Chanthavongsa、 Khamaeng Sayaket、 Bouapha Ngothachack、 Akio Tani、 Noppon Lertwattanasakul、 Somchanh Bounphanmy、 Savitree Limtong and Mamoru Yamada

    Final Joint Seminar of CCP  2018年12月2日 

     詳細を見る

    開催年月日: 2018年12月2日 - 2018年12月4日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Yamaguchi University  

    researchmap

  • ホウレンソウにおける収穫後のMethylobacterium属細菌の挙動とその成育を制御する微生物の探索

    阪口由佳、繁原安美、王倫、谷明生、中野浩平、稲垣瑞穂、島田昌也、早川享志、中川智行

    美味技術学会  2018年11月8日 

     詳細を見る

    開催年月日: 2018年11月8日 - 2018年11月9日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:大阪  

    researchmap

  • Molecular mechanism of methylotaxis in Methylobacterium aquaticum strain 22A 国際会議

    Yuuki Haruna and Akio Tani

    3rd International Conference on Biologically Active Substances  2018年10月16日 

     詳細を見る

    開催年月日: 2018年10月16日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Okayama International Center  

    researchmap

  • Functional analysis of lanthanide inducible proteins of Methylobacterium aquaticum 22A 国際会議

    Yoshiko Fujitani and Akio Tani

    3rd International Conference on Biologically Active Substances  2018年10月16日 

     詳細を見る

    開催年月日: 2018年10月16日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Okayama International Center  

    researchmap

  • Formaldehyde oxidation in lanthanide-dependent methylotrophy in Methylobacterium aquaticum strain 22A 国際会議

    Patcha Yanpirat and Akio Tani

    3rd International Conference on Biologically Active Substances  2018年10月16日 

     詳細を見る

    開催年月日: 2018年10月16日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:Okayama International Center  

    researchmap

  • ランタノイド依存型メタノール脱水素酵素XoxF1の酵素活性と安定性はランタノイド種に依存する

    王 倫、菅沼宗矢、日比野歩美、谷明生、三井亮司、海老原章郎、 岩本悟志、稲垣瑞穂、島田昌也、早川享志、中川智行

    農芸化学会中部支部 支部例会  2018年9月15日 

     詳細を見る

    開催年月日: 2018年9月15日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:名古屋  

    researchmap

  • Methylobacterium extorquens AM1のランタノイド依存型メタノールデヒドロゲナーゼの解析

    吉川友理、北村純一、矢野嵩典、中川智行、谷 明生、三井亮司

    第70回日本生物工学会大会  2018年9月5日 

     詳細を見る

    開催年月日: 2018年9月5日 - 2018年9月7日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:関西大学  

    researchmap

  • Multiple formaldehyde oxidation pathways in Methylobacterium aquaticum strain 22A

    Patcha Yanpirat and Akio Tani

    日本微生物生態学会 第32回大会  2018年7月11日 

     詳細を見る

    開催年月日: 2018年7月11日 - 2018年7月13日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:沖縄コンベンションセンター  

    researchmap

  • Molecular mechanism of methylotaxis in Methylobacterium aquaticum strain 22A

    Yuuki Haruna、 Junichi Kato、 and Akio Tani

    日本微生物生態学会 第32回大会  2018年7月11日 

     詳細を見る

    開催年月日: 2018年7月11日 - 2018年7月13日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:沖縄コンベンションセンター  

    researchmap

  • Functional analysis of lanthanide inducible protein in Methylobacterium aquaticum 22A

    Yoshiko Fujitani and Akio Tani

    日本微生物生態学会 第32回大会  2018年7月11日 

     詳細を見る

    開催年月日: 2018年7月11日 - 2018年7月13日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:沖縄コンベンションセンター  

    researchmap

  • Methylobacterium属細菌におけるメタノール走化性センサーの機能解析

    春名優希、加藤純一、谷明生

    日本農芸化学会  2018年3月15日 

     詳細を見る

    開催年月日: 2018年3月15日 - 2018年3月18日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:名古屋 名城大学  

    researchmap

  • Methylobacterium aquaticum strain 22Aにおけるメタノール脱水素酵素遺伝子のランタノイド依存的制御

    平賀翔大、藤谷良子、谷明生

    日本農芸化学会  2018年3月15日 

     詳細を見る

    開催年月日: 2018年3月15日 - 2018年3月18日

    記述言語:日本語   会議種別:口頭発表(一般)  

    開催地:名古屋 名城大学  

    researchmap

  • Draft genome and description of Novimethylophilus kurashikiensis gen. nov. sp. nov., a new lanthanide-dependent methylotrophic species of Methylophilaceae

    Haoxin Lyu and Akio Tani

    日本農芸化学会  2018年3月15日 

     詳細を見る

    開催年月日: 2018年3月15日 - 2018年3月18日

    記述言語:英語   会議種別:口頭発表(一般)  

    開催地:名古屋 名城大学  

    researchmap

  • フローサイトメトリを用いた代謝産物の蛍光に基づく微生物単離法の開発

    日本農芸化学会大会  2009年 

     詳細を見る

  • Flow Cytometry and Cell-Sorting Method for Isolating Live Bacteria with Meta-Cleavage Activity on Dihydroxy Compounds of Biphenyl

    APBioChEC'09 Biotechnology for sustainable Development  2009年 

     詳細を見る

  • IncP-7群カルバゾール分解プラスミドpCAR1の宿主域の解析

    環境バイオテクノロジー学会  2009年 

     詳細を見る

  • 出芽酵母のホルムアルデヒドストレス耐性機構におけるTps1の役割

    日本生物工学会  2009年 

     詳細を見る

  • 「環境細菌の生き様を探る」

    化学工学会東海支部 第42回研究交流セミナー  2009年 

     詳細を見る

  • IncP-7群カルバゾール分解プラスミドpCAR1の宿主域の解析

    農芸化学会関東支部大会  2009年 

     詳細を見る

  • MALDI-TOF/MSによるMethylobacterium属細菌のタイピングと生化学的特徴

    日本生物工学会  2009年 

     詳細を見る

  • MALDI-TOF/MS-based phylogenic analysis of Methylobacterium species collected from plant samples

    MARCO シンポジウム2009 モンスーンアジアにおける農業環境問題と研究の課題  2009年 

     詳細を見る

  • 酵母のホルムアルデヒド耐性におけるトレハロース合成遺伝子の関与

    日本農芸化学会大会  2009年 

     詳細を見る

  • MALDI-TOF/MSによるMethylobacterium属細菌のタイピング

    日本農芸化学会大会  2009年 

     詳細を見る

  • スナゴケの生育を促進する微生物

    日本農芸化学会中四国支部第21回講演会  2008年 

     詳細を見る

  • Racomitrium canescens as a material for greening and physiological study

    MOSS 2008, The annual international symposium for moss experimental research  2008年 

     詳細を見る

  • カルバゾール分解プラスミドの環境中における挙動の解析

    日本農芸化学会中四国支部第21回講演会  2008年 

     詳細を見る

  • Plant-growth promotion by methylobacteria

    Gordon Conference Molecular basis of microbial one-carbon metabolism  2008年 

     詳細を見る

  • 土壌環境中の細菌の検出技術

    日本生物工学会2008年度大会 シンポジウム(土壌環境での細菌の生き様を探る)  2008年 

     詳細を見る

  • スナゴケの生育を促進する微生物

    日本生物工学会2008年度大会  2008年 

     詳細を見る

  • Rhodotorula glutinisにおけるアルミニウム耐性機構とミトコンドリ ア活性

    2007.3.24-27  2007年 

     詳細を見る

  • 出芽酵母のアセトアルデヒド誘導性因子の探索と解析

    2007年度 日本生物工学会大会  2007年 

     詳細を見る

  • 赤色酵母Rhodotorula glutinis IFO1125株のアルミニウム耐性におけるミトコンドリアの役割

    農芸化学会中四国大会  2007年 

     詳細を見る

  • The physiological role of glutathione-S-transferase in the downstream of peg operon in Sphingopyxis macrogoltabida

    2007年度日本生物工学会  2007年 

     詳細を見る

  • Xenobiotic polymer degradation by Sphingomonads.

    JSPS-NRCT Core university program (1998-2008) on Development of thermotolerant microbial resources and their applications  2007年 

     詳細を見る

  • 出芽酵母のアセトアルデヒド応答とオレイン酸合成の制御

    日本農芸化学会北海道支部 合同学術講演会  2007年 

     詳細を見る

  • Dual regulation of a polyethylene glycol-degradative operon by AraC-type and GalR-type regulator in Sphingopyxis macrogoltabida strain 103

    The 5 th JSPS-NRCT joint seminar on development of thermotolerant microbial resources and their applications  2006年 

     詳細を見る

  • Involvement of acyl-CoA synthetase and glutathione-S-transferase in PEG degradation by Sphingomonads

    The 5 th JSPS-NRCT joint seminar on development of thermotolerant microbial resources and their applications  2006年 

     詳細を見る

  • Sphingomonas 属細菌によるポリエチレングリコール分解オペロンの保存と転写制御

    日本生物工学会 第58回  2006年 

     詳細を見る

  • Aerobic metabolism of xenoestrogenic short ethoxy chain-nonylphenol by bacteria able to grown on short ethoxy chain-nonylphenol and nonylphenol

    Pacifichem 2005  2005年 

     詳細を見る

  • Adaptive Al-resistance in Rhodotorula glutinis IFO1125

    XXII. International conference on yeast genetics and molecular biology  2005年 

     詳細を見る

  • Regulation of genes relevant to microbial degradation of xenobiotic polymers,

    ACS meeting  2005年 

     詳細を見る

  • Genes relevant to the degradation of polyethylene glycol in Sphingomonads

    農芸化学会2005年度大会  2005年 

     詳細を見る

  • ポリエチレングリコール(PEG)代謝に関わるSphingomonas macrogoltabidus strain 103 由来ニコチノプロテイン・アルデヒド脱水素酵素遺伝子の発現

    農芸化学会2005年度大会  2005年 

     詳細を見る

  • 赤色酵母Rhodotolura glutinisのAl耐性獲得機構におけるarsenite translocating ATPaseの役割

    農芸化学会2005年度大会  2005年 

     詳細を見る

  • 赤色酵母Rhodotorula glutinisのAl耐性獲得機構

    農芸化学会2005年度大会  2005年 

     詳細を見る

  • Degradation of nonylphenol mono- and diethoxylates by Ensifer sp. AS08

    日本農芸化学会中四国支部講演会  2005年 

     詳細を見る

  • Transcription of the genes involved in polyethylene glycol degradation by Sphingomonas macrogoltabidus No. 103

    2nd Circular 4th JSPS-NRCT Joint Seminar on Development of Thermotolerant Microbial Resources and Their Applications  2004年 

     詳細を見る

  • ポリエチレングリコール代謝遺伝子構造とその制御

    平成16年度日本農芸化学会  2004年 

     詳細を見る

  • Bacterial degradation of nonylphenol mono-and diethoxylates

    平成16年度日本農芸化学会  2004年 

     詳細を見る

  • Rhodotorula glutinis の後成的アルミニウム耐性機構

    平成16年度日本農芸化学会  2004年 

     詳細を見る

  • ポリエチレングリコール(PEG)代謝に関わるエーテル結合開裂酵素の遺伝子解析

    平成16年度日本農芸化学会  2004年 

     詳細を見る

  • Adaptive Acquirement of Heritable Aluminum Resistance by Rhodotorula glutinis IFO1125

    American Society for Microbiology general meeting  2004年 

     詳細を見る

  • Rhodotorula glutinis の後成的アルミニウム耐性獲得機構

    日本農芸化学会中四国支部第9回講演会(例会)  2004年 

     詳細を見る

  • Adaptive aquirement of Al resistance in yeast Rhodotorula glutinis IFO 1125.

    低pH領域における植物と土壌の相互作用に関する国際会議  2004年 

     詳細を見る

  • Sphingomonas terrae 由来ポリエチレングリコール脱水素酵素の構造と機能解析

    日本生物工学会2004年次大会  2004年 

     詳細を見る

  • Marine biosurfactant: Purification and characterization of a biosurfactant produced by Myroides odoratus SMT1 isolated from seawater in Thailand

    農芸化学会大会  2003年 

     詳細を見る

  • 赤色酵母の後成的アルミニウム耐性獲得機構

    農芸化学会大会  2003年 

     詳細を見る

  • グラム陽性菌Pseudonocardia sp. strain K1のポリエチレングリコール(PEG)代謝

    農芸化学会大会  2003年 

     詳細を見る

  • Purification and gene cloning of oxidized polyvinyl alcohol hydrolase from Sphingomonas sp. 113P3

    農芸化学会大会  2003年 

     詳細を見る

  • Rhodotorula glutinis におけるアルミニウム耐性獲得機構

    日本生物工学会  2003年 

     詳細を見る

  • Adaptive acquirement of aluminum resistance in Rhodotorula glutinis

    日本生化学会  2003年 

     詳細を見る

  • Sphingomonas sp. 由来ポリビニルアルコール脱水素酵素(PVA-DH)遺伝子のクローニング及びその諸性質

    農芸化学会大会  2003年 

     詳細を見る

  • Adaptive acquirement of heritable aluminium tolerance by epigenetic regulation

    Fifth Keele Meeting on Aluminium  2003年 

     詳細を見る

  • Adaptive acquirement of heritable aluminium tolerance by epigenetic regulation.

    Fifth Keele Meeting on Aluminium Aluminium in Life: From acid rain to Alzheimer's disease  2003年 

     詳細を見る

  • Sphingomonas terrae 由来ポリエチレングリコール脱水素酵素(PEG-DH)におけるH467, C472およびN511の役割

    日本農芸化学会2002年度大会  2002年 

     詳細を見る

  • 赤色酵母の後成的アルミニウム耐性獲得機構

    環境微生物研究会主催、藪田セミナー講演会  2002年 

     詳細を見る

  • The first step in the degradation of polyethers proceeds via alcohol dehydrogenases

    9th International symposium on the genetics of industrial microorganisms  2002年 

     詳細を見る

  • Sphingomonas terrae 由来ポリエチレングリコール脱水素酵素におけるH467, C472, およびN511の役割

    日本農芸化学会2001年度関西・西日本・中四国支部合同大会  2001年 

     詳細を見る

  • iso-アルカン分解菌の探索と分解菌の系統分類

    日本生物工学会2001年度大会  2001年 

     詳細を見る

  • 長鎖n-アルカン資化性細菌Acinetobacter sp. M-1株に見いだした長鎖Acyl-CoA reductaseの生理的役割

    日本農芸化学会2001年度大会  2001年 

     詳細を見る

  • ガス状短鎖アルカン資化性細菌Gordonia sp. TY-5 株の分離とプロパン代謝

    日本農芸化学会2001年度大会  2001年 

     詳細を見る

  • スフィンゴモナス属細菌の細胞表層構造と合成高分子物質の分解

    シンポジウム科学研究費補助金基盤研究(c) (企画調査)  2001年 

     詳細を見る

  • Acinetobacter sp. M-1株によるワックスエステル生産

    日本農芸化学会2001年度関西・西日本・中四国支部合同大会  2001年 

     詳細を見る

  • アルカン資化性細菌Acinetobacter sp. M-1由来acyl-CoA reductaseのクローニング

    日本生物工学会2000年度大会  2000年 

     詳細を見る

  • アルカン資化性細菌Acinetobacter sp. M-1由来acyl-CoA reductaseのクローニング

    日本生物工学会2000年度大会  2000年 

     詳細を見る

  • 長鎖n-アルカン分解菌Acinetobacter sp. M-1由来の2つのアルカンヒドロキシラーゼの諸性質

    日本生物工学会2000年度大会  2000年 

     詳細を見る

  • 長鎖n-アルカン分解菌Acinetobacter sp. M-1由来の2つのアルカンヒドロキシラーゼの諸性質

    日本生物工学会2000年度大会  2000年 

     詳細を見る

  • 長鎖n-アルカン分解菌Acinetobacter sp. M-1由来の2つのアルカンヒドロキシラーゼの諸性質

    日本農芸化学会2000年度大会  2000年 

     詳細を見る

  • 長鎖n-アルカン分解菌Acinetobacter sp. M-1由来の2つのアルカンヒドロキシラーゼの諸性質

    日本農芸化学会2000年度大会  2000年 

     詳細を見る

  • Oxidation of Long-Chain n-alkanes by Acinetobacter sp. M-1: Existence of Two Alkane Hydroxylases

    Fifth International Symposium on Environmental Biotechnology  2000年 

     詳細を見る

  • Long-Chain Aldehyde Dehydrogenase That Participates in n-Alkane Utilization and Wax Ester Synthesis of Acinetobacter sp. M-1

    Fifth International Symposium on Environmental Biotechnology  2000年 

     詳細を見る

  • Acinetobacter sp. M-1由来の2つのアルカンヒドロキシラーゼのクローニング

    日本生物工学会1999年度大会  1999年 

     詳細を見る

  • アルカン資化性細菌Acinetobacter sp. M-1に見出した長鎖アルデヒドデヒドロゲナーゼの生理的役割

    日本生物工学会1999年度大会  1999年 

     詳細を見る

  • 長鎖n-アルカン分解菌Acinetobacter sp. M-1 株のAcyl-CoA脱水素酵素遺伝子のクローニングと酵素化学的諸性質

    日本農芸化学会1999年度大会、平成11年4月  1999年 

     詳細を見る

▼全件表示

産業財産権

  • エルゴチオネインの産生方法

    谷 明生

     詳細を見る

    出願人:国立大学法人 岡山大学

    出願番号:特願2016-122379  出願日:2016年6月21日

    特許番号/登録番号:特許6758703  登録日:2020年9月4日 

    権利者:国立大学法人岡山大学

    researchmap

Works(作品等)

  • 岡山発これからの屋上ガーデン

    2009年

     詳細を見る

    作品分類:芸術活動  

    researchmap

  • 「屋上緑化用植物スナゴケの生育を促進する微生物」

    2008年

     詳細を見る

    作品分類:芸術活動  

    researchmap

受賞

  • 論文賞

    2020年3月   日本農芸化学会   Bacteria with natural chemotaxis towards methanol revealed by chemotaxis fishing technique

    Yosef Hamba Tola, Yoshiko Fujitani, Akio Tani

     詳細を見る

  • 2015年度発酵と代謝研究奨励賞

    2015年6月   バイオインダストリー協会   ユニークな抗酸化性アミノ酸、エルゴチオネインの微生物生産

    谷 明生

     詳細を見る

  • ポスター賞

    2012年9月   IBS 2012 15th International Biotechnology Symposium and Exhibition, EXCO, Daegu, Korea   Monitoring system for biofilm architecture and development using a multichannel microdevice

    Sanchez Z, Ota T, Tani A, Kimbara K

     詳細を見る

  • 岡山大学若手トップリサーチャー賞

    2012年3月   岡山大学  

    谷 明生

     詳細を見る

  • 研究企画賞

    2010年9月   日本農芸化学会   品種の異なるオオムギに共生するメタノール資化性菌の網羅的ライブラリ作製と宿主生育促進効果の解析

    谷 明生

     詳細を見る

  • 岡山工学振興会科学技術賞

    2002年  

     詳細を見る

    受賞国:日本国

    researchmap

  • 日本生物工学会、論文賞

    1997年  

     詳細を見る

    受賞国:日本国

    researchmap

▼全件表示

共同研究・競争的資金等の研究

  • ランタノイド依存メタノール代謝系制御機構の分子間相互作用解析

    研究課題/領域番号:21H02105  2021年04月 - 2024年03月

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    谷 明生

      詳細を見る

    配分額:17940000円 ( 直接経費:13800000円 、 間接経費:4140000円 )

    researchmap

  • Wastewater treatment by-products to improve feed crop production in Qatar

    2021年04月 - 2023年03月

    Qatar-Japan Research Collaboration Application (QJRC) 

      詳細を見る

    担当区分:研究代表者 

    researchmap

  • オオムギ根の内生菌の生態解明とその機能開発

    2020年 - 2021年

    日本環境財団 

      詳細を見る

    担当区分:研究代表者 

    researchmap

  • 根圏生態系の季節変動から紐解く二毛作体系の生物学的な持続性

    研究課題/領域番号:19KT0011  2019年07月 - 2022年03月

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    谷 明生, 山本 敏央, 山地 直樹, 山下 純, 門田 有希, 中川 智行, 最相 大輔, 持田 恵一

      詳細を見る

    配分額:18590000円 ( 直接経費:14300000円 、 間接経費:4290000円 )

    植物の生育は植物の遺伝背景や環境要因等の相互作用の結果として捉えることができる。根圏微生物叢は植物の生育に多大な影響を与えており、逆に、植物の遺伝的背景や環境要因にも規定され、相互作用すると考えられる。本研究の目的は、圃場環境において微生物叢を含むあらゆるパラメータをデータ化し、それらの要因間のネットワークを可視化し,農業生態系としての季節変動動態を理解することである。
    研究所実験圃場の慣行区と無施肥区において、アルミニウムに対する感受性の異なるイネとオオムギの品種対を対象に、隔週で各条件3植物個体ずつ(イネは6個体)サンプリングした。環境要因として、フィールドサーバーを用いて日照、降雨、温度、湿度、風向、風速を測定した。土壌環境データとして根圏土壌を採取し、ICP-MSによる水抽出可能な元素の組成を測定し、土壌センサを用いて土壌pHを測定した。さらに土壌の根圏微生物DNAを精製し、16S rRNA遺伝子アンプリコンシーケンスを行った。
    施肥の有無によりイオン等の動態は変化し、pHは慣行区で低く、カリウム、リンは慣行区で高濃度であるが早い時期に消費された。慣行区の硫黄、無施肥区のマグネシウム、マンガン、銅が落水により増加した。同じく落水によると考えられるヒ素の増加、カドミウムの低下が無施肥区で見られた。
    微生物叢は、イネではMassilia属、Pseudomonadaceae、Oxalobacteraceaeがstar1で多い傾向が見られた。オオムギでは根圏土壌と根のサンプルで相違が見られ、根のサンプルにのみJanthinobacterium, Methylibium属細菌が多く存在することが分かった。これらはオオムギ根のエンドファイトであると考えられ、そのオオムギに対する共生機構や成長への影響に興味が持たれる。

    researchmap

  • 植物共生細菌のメタノール走化性の機構と重要性の解明

    2019年 - 2021年

    公益財団法人 ウエスコ学術振興財団  研究活動費助成事業 

      詳細を見る

  • 微生物におけるランタノイド元素の意義

    研究課題/領域番号:18H02129  2018年04月 - 2021年03月

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    谷 明生

      詳細を見る

    配分額:17680000円 ( 直接経費:13600000円 、 間接経費:4080000円 )

    Methylobacterium属細菌は、植物が排出するメタノールを利用して、その地上部表面で優占する重要な植物共生細菌である。メタノールの初発酸化酵素であるメタノール脱水素酵素(MDH)はカルシウム(Ca)依存であることが知られていたが、別のホモログMDHがランタノイド(Ln)依存であることが新しく発見された。これはLnが酵素の補因子となる初めての例である。また、本属細菌はLn依存MDHを優先的に利用し、Lnが存在しない時のみCa依存MDHを用いること、Ln依存MDHの方が祖先型であること等から、本属細菌にとっては、Lnの方がより重要な金属であると推察される。本研究では、このLnに応じたMDH遺伝子発現制御機構の解明と、Ln存在下での各種遺伝子発現応答の解析を通じて、生物におけるLnの意義を明らかにすることを目的とする。
    モデルとして用いたM. aquaticum 22A株はMDHの産物であるホルムアルデヒドの代謝系としてH4MPT経路とグルタチオン依存の経路の二種類を持っている。遺伝学的解析から、H4MPT経路が主なホルムアルデヒド酸化系であるが後者もホルムアルデヒド酸化に関わること、XoxFはホルムアルデヒドも酸化すること、またAM1株で知られていたExaFのホモログが存在してエタノールとホルムアルデヒド酸化に関わることを見いだした。
    また、Ln存在下発現量が亢進し、Lnを結合すると考えられるランモジュリンタンパク質(LanM)についても遺伝学的・生化学的に解析を行い、確かにLnを結合すること、XoxFではなくMxaFの発現に関わっていることや、細胞内へのLnの取り込みに関わることを見いだした。
    またMxaFの発現調節に関わるmxbDM遺伝子について遺伝学的解析を行い、MxbMがLn非存在下でリン酸化され、MxaFの発現制御をONにすることを見いだした。

    researchmap

  • ランタノイド依存メタノール脱水素酵素の多様性と制御機構の解明

    研究課題/領域番号:15H04476  2015年04月 - 2018年03月

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    谷 明生, 三井 亮司, 中川 智行, 呂 好新

      詳細を見る

    配分額:17160000円 ( 直接経費:13200000円 、 間接経費:3960000円 )

    Methylobacterium属細菌が持つ複数のメタノール脱水素酵素(MDH)の個々の役割を遺伝学的に調べ、カルシウム依存型MDHであるMxaFとランタノイド(Ln)型MDH、XoxF1がメタノール、エタノールでの生育に必須であること、他のADHがプロパノールなどのアルコールへの生育に関与することが分かった。またMxaF, XoxF1の発現切り替え機構に関わるMxbDタンパク質について遺伝学的解析を行い、MxbDがMxaFの発現に必要であること、XoxFの発現には必要無いこと、またMxbDのHAMPドメインにおける自然突然変異がMxaF発現のXoxF発現依存性を解除することを明らかにした。

    researchmap

  • メタノールをメディエータとした微生物と植物間の新しい相互作用モデルの確立

    研究課題/領域番号:26660074  2014年04月 - 2017年03月

    日本学術振興会  科学研究費助成事業 挑戦的萌芽研究  挑戦的萌芽研究

    三井 亮司, 田中 三男, 中川 智行, 谷 明生, 南澤 究

      詳細を見る

    配分額:3770000円 ( 直接経費:2900000円 、 間接経費:870000円 )

    植物の生育に伴い放出されるメタノールが葉上・根圏の微生物群にとって重要な役割を持つことを明らかにしてきた。本研究課題では軽ランタノイド(ランタン・セリウム・プラセオジム・ネオジム)が土壌に広範に分布し、メタノール資化性細菌の初発酵素メタノールデヒドロゲナーゼの補欠因子となっていることに着目した。植物葉上優占種Methylobacterium属細菌や窒素固定マメ化植物根粒形成菌がランタノイドの存在下で植物から放出されるメタノールをメディエータとして利用し、植物との共生関係を構築していることを示唆する結果が得られた。

    researchmap

  • 植物表層常在菌と植物との相互認識機構の解明

    研究課題/領域番号:25660060  2013年04月 - 2015年03月

    日本学術振興会  科学研究費助成事業 挑戦的萌芽研究  挑戦的萌芽研究

    谷 明生

      詳細を見る

    配分額:3900000円 ( 直接経費:3000000円 、 間接経費:900000円 )

    植物の葉面に存在する常在菌であるSphingomonas属細菌とMethylobacterium属細菌は、それぞれ植物に何の悪影響ももたず生存可能である。さらに前者は病原性細菌の生育を抑える効果が知られている。本研究では植物がどのように前者を認識しているか、また、前者が病原性細菌の生育を抑える機構について検討した。まずSphingomonas属細菌の細胞、鞭毛タンパク質、植物が認識する鞭毛ペプチドはそれぞれ植物の免疫応答を引き起こすことを確認した。また病原性細菌の生育抑制には細胞外に分泌される何らかの物質、おそらく鉄の奪い合いに関与する物質が直接効果を持っていることを明らかにした。

    researchmap

  • 植物・昆虫インターフェイスで繰り広げられる分子間相互作用

    研究課題/領域番号:24570026  2012年04月 - 2015年03月

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    IVAN Galis, 新屋 友規, 谷 明生

      詳細を見る

    配分額:5460000円 ( 直接経費:4200000円 、 間接経費:1260000円 )

    本研究において、イネ-植食性昆虫間の相互作用解析を行う上で、有用な研究基盤を構築した。イネの防御応答において、OsJAR1を介して植物ホルモンであるジャスモン酸イソロイシン(JA-Ile)が蓄積すること、またJAやJA-Ileの蓄積は、主要な生合成経路であるリノレン酸量により制御されることを示した。一方で傷害応答との比較解析より、咀嚼性植食性昆虫の吐き戻し液に含まれるエリシターに特異的に応答して蓄積する、新規なフェノールアミド化合物を同定した。さらに、フェノールアミド化合物やROS蓄積を指標に、クサシロキヨトウの吐き戻し液に含まれる、新規と推定されるエリシターの部分精製を行った。

    researchmap

  • 植物に共生するメタノール資化性細菌の多様性と共生機構の解明

    研究課題/領域番号:23688012  2011年04月 - 2014年03月

    日本学術振興会  科学研究費助成事業 若手研究(A)  若手研究(A)

    谷 明生, 中川 智行, 三井 亮司

      詳細を見る

    配分額:17550000円 ( 直接経費:13500000円 、 間接経費:4050000円 )

    植物に多く共生するMethylobacterium属細菌の系統分類に関しては四株について新種提唱を行った。生育促進に関わる植物ホルモンについて分析し、サイトカイニンが最も重要であることを示唆する結果を得た。メタノール脱水素酵素(MDH)の補酵素が気孔を開く活性を持っており、その作用機構として活性酸素の除去にあることを見いだしている。MDHのホモログの中に希土類元素を要求するものを見いだし、機能解析を行った。イネをモデルとして本属細菌の種レベルでの同定を行い、イネの種子に含まれる本属細菌の種は、イネの遺伝型よりも栽培条件に影響されていることを示唆する結果を得た。

    researchmap

  • 植物共生メタノール資化性細菌の葉上における生存戦略に関する研究

    研究課題/領域番号:23580122  2011年 - 2013年

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    三井 亮司, 田中 三男, 中川 智行, 谷 明生

      詳細を見る

    配分額:5460000円 ( 直接経費:4200000円 、 間接経費:1260000円 )

    メチロトローフと呼ばれるC1化合物資化性菌はメタノールなどのC1化合物を炭素源として生育するが、長く環境中でのメタノールの供給源ははっきりしていなかった。近年、植物から多量のメタノールが放出されていることが明らかになり、植物とメチロトローフとの関係が広く知られるようになってきた。本研究では植物葉上や根圏に生育するメタノール資化性細菌が互いに化学物質を介してコミュニケーションしており、これにより植物上で共生関係を築くことを明らかにした。

    researchmap

  • 新手法によるAzospirillum属細菌の網羅的分離と分類

    研究課題/領域番号:23658080  2011年 - 2012年

    日本学術振興会  科学研究費助成事業 挑戦的萌芽研究  挑戦的萌芽研究

    谷 明生, 中川 智行, 三井 亮司

      詳細を見る

    配分額:3900000円 ( 直接経費:3000000円 、 間接経費:900000円 )

    Azospirillum属細菌は植物根圏に共生し、窒素固定を行い、植物の生育を促進可能な細菌の一群である。本属細菌の分離には工夫が必要で、また分類系統も良く確立しておらず、共生する植物との関連性も分かっていない。本研究では広く植物から本属細菌を分離することと、質量分析法を用いた新しい手法で効率よく網羅的に分類すること、同時に新種菌を見いだし分類系統に新たな知見を加えることを目的としている。本属細菌の分離には窒素源を含まない培地を用いた微好気条件が重要であることを見いだし、約60株の本属細菌を分離した。質量分析により正確な分類を行った。またイネに対して約20%の生育促進効果を持つ菌を分離した。

    researchmap

  • メタノール資化性細菌のゲノム解析による植物生育促進作用の解明

    研究課題/領域番号:21780074  2009年 - 2010年

    日本学術振興会  科学研究費助成事業 若手研究(B)  若手研究(B)

    谷 明生, 金原 和秀

      詳細を見る

    配分額:4680000円 ( 直接経費:3600000円 、 間接経費:1080000円 )

    様々な植物に高い生育促進効果を持つMethylobacterium sp.22A株を選抜し、ゲノム解析を行った。これにより、コンティグ数490、平均コンティグサイズ16kbpのドラフトゲノムデータが得られた。ゲノムサイズは7.7Mbpで、プラスミドは6つ存在した。植物との相互作用に重要と考えられるメタノール資化経路、窒素固定、ビタミン合成系などの遺伝子を同定した。現在重要な遺伝子の破壊株の作成中である。

    researchmap

  • スフィンゴモナス属細菌のポリエチレングリコール分解メガプラスミドの環境中挙動

    研究課題/領域番号:19780060  2007年 - 2008年

    日本学術振興会  科学研究費助成事業 若手研究(B)  若手研究(B)

    谷 明生, 金原 和秀, 河合 富佐子

      詳細を見る

    配分額:3710000円 ( 直接経費:3500000円 、 間接経費:210000円 )

    人工化合物であるポリエチレングリコール(PEG)の有力な分解菌であるSphingomonas属細菌はPEG分解遺伝子をプラスミドにコードしている。このプラスミドは接合伝達で他の菌にも移ることが示唆されているが、その宿主特異性などは明らかではない。本研究ではPEG分解菌が持つプラスミドの環境中での挙動を解析するにあたり基礎的な知見を蓄積し、PEGの完全分解に関わる遺伝子群を明らかにした。

    researchmap

  • 芳香族化合物分解好熱菌の代謝酵素群のネットワーク形成に関する研究

    研究課題/領域番号:18580077  2006年 - 2008年

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    金原 和秀, 谷 明生, 河合 富佐子, 清水 頼子

      詳細を見る

    配分額:3850000円 ( 直接経費:3400000円 、 間接経費:450000円 )

    芳香族化合物を分解する微生物は、その能力を進化の過程で獲得してきたものと考えられている。本研究では、これまで解析された例が少ない好熱性細菌に着目し、ナフタレン代謝酵素遺伝子群の解析を行うことを目的とした。その結果、遺伝子の単離とナフタレンによる誘導性を見出すことが出来た。しかし、ナフタレンを代謝する過程で生理活性が著しく低下することが分かり、酵素機能と代謝ネットワークの詳細を解析するにはいたらなかった。

    researchmap

  • アルミニウム耐性菌による酸性土壌の改良とアルミニウム耐性の分子機構

    研究課題/領域番号:17580065  2005年 - 2007年

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    河合 富佐子, 金原 和秀, 谷 明生, 清水 頼子

      詳細を見る

    配分額:3610000円 ( 直接経費:3400000円 、 間接経費:210000円 )

    近年酸性雨のため生産性土壌でも酸性化が進んでいる。土壌の酸性化は金属を溶出させるが、Al3+は生物学的に不要で、生物毒性があり、農業生産や自然に影響を与える。植物に対するAl毒性はよく研究されているが、土壌微生物に対するAlの影響に関する研究、特にAl3+が存在するpH3以下の条件で生育可能な耐性菌の研究はほとんどない。Al3+の土壌微生物に対する影響とその耐性を総合的に解明し、土壌の物性と植物の生育の両面から酸性土壌の改良に応用することは食糧生産、環境保護と改善に大きく貢献する。本研究では、1) Penicillium janthinellum F-13による土壌改善、2)P.chrysogenum IFO4626由来Al耐性変異株からえたAl遺伝子をタバコに導入して調べる、3)タイ及び国内茶畑土壌の微生物群集解析を行う、4)Rhodotorula glutinis IFO1125の新規Al耐性機構の解明を行うことを目的とした。1)に関しては、いくつかの酸性土壌で実地検証した結果を論文1)としてまとめた。また、胞子の耐久性、F-13株の接種条件等についてもデータを得た。2)は2種類の遺伝子を選んで、タバコを形質転換し、T2世代の種子を発芽させて、Ruakura培地(pH4.3)でAlCl3を0-50μM添加条件で根の伸長を測定した。しかし、有意差のある結果はえられなかった。培地を変えて調べるとともに、植物と微生物ではAl耐性遺伝子の役割が異なる可能性もあるので、他の遺伝子についても調べる。3)通常の土壌と酸性土壌の微生物群集比較解析の結果、酸性化は微生物叢の構成を大きく変える可能性が示唆され,酸性雨は土壌微生物生態にも大きく影響することが推定された。4)Al毒性は活性酸素種による脂質、蛋白質の酸化によると推定した。Al暴露に対する耐性株はミトコンドリの数と機能を強化して対処していることを明らかにし、Microbiologyに受理された。さらにAlによる発現促進は核遺伝子でも観察され、その中から3遺伝子を選んでクローニングし、S.cerevisiaeの相同遺伝子欠損株に導入して耐性の増加を確認し、現在投稿中である。

    researchmap

  • Sphingomonas属細菌の細胞表層機能と合成高分子の分解

    研究課題/領域番号:14560068  2002年 - 2004年

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    河合 富佐子, 谷 明生

      詳細を見る

    配分額:2700000円 ( 直接経費:2700000円 )

    本研究ではポリエチレングリコール(PEG)ポリビニルアルコール(PVA)資化性Sphingomonas属細菌を用いて、専ら分子生物学的なアプローチで分解遺伝子とそのオペロン構成及び発現解析を行った。
    PEG脱水素酵素遺伝子(pegA)を中心に上下流合わせて約14kbの塩基配列を解読した。ORFはtransposases (A, B), Ton B receptor, aldehyde dehydrogenase, permease, acylCoA ligase, Ara C-type regulator, transposases (A, B)に該当し、この配列はS. terraeおよびS. macrogoltabidus strains 103 & 203に99%以上の相同性で保存さていた。これらの遺伝子群はトランスポゾンを除いてPEG培地で誘導的に発現した。また、逆向きに挿入されたAra C-type regulatorを除いてmonocistronicに発現することをdot-blot hybridizationおよびRT-PCRで確認し、5つがPEG分解オペロンを構成すると判断した。一方、S. macrogoltabidus 203のpegA上流には別のトランスポゾンが挿入され、pegAのの構成的な発現はトランスポゾンの挿入でpromoter領域が分断されたものと結論づけた。他方、aldehyde dehydrogenase遺伝子の発現タンパク質について酵素の特性を調べ、PEG-aldehyde dehydrogenaseであることを確認した。本酵素は結合型のNADPを有するnicotinoprotein aldehyde dehydrogenaseとしてはじめての報告である。
    他方、酸化PVA加水分解酵素(OPH)を精製し、精製酵素のアミノ酸配列を下に遺伝子をクローニングした。遺伝子は大腸菌で発現できたが、酵素はinclusion bodyを形成した。この遺伝子の下流にPVA dehydrogenase (pvaA)とcytochrome cのORFが存在することを確認した。遺伝子ophとpvaA間にはほとんどspaceがなく、共発現していると考えられる。
    表題としてあげた細菌の細胞表層構造はPEG(PVA)培地と栄養培地では電顕観察で大きく異なる。この変化とオペロンの遺伝子構成を結論づけるには至らなかったが、さらにそれぞれの上下流の塩基配列を解読中であり、細胞表層にコードされていると思われる遺伝子が含まれるので、これらの機能解析を進めて細胞表層における高分子の認識/取込みと遺伝子制御を関連づけるのが今後の課題である。

    researchmap

  • 細菌によるアルカン分解機構の解明とそれを利用した鯨油に代わるワックス類の生産

    研究課題/領域番号:14760050  2002年 - 2003年

    日本学術振興会  科学研究費助成事業 若手研究(B)  若手研究(B)

    谷 明生

      詳細を見る

    配分額:1900000円 ( 直接経費:1900000円 )

    原油中の主成分であるn-アルカンを分解できる微生物については細菌・酵母・カビに至るまで数多くの報告があるが、現在までに知られている中でAcinetobacter sp.M-1株は最も長鎖の成分(炭素数30以上)を分解できる細菌として例を見ない分解特性を持っている。本菌は常温で個体である成分にまで旺盛に生育することから、その強力な分解活性と、代謝産物であるワックスエステルの合成力とを用いて廃油や自然環境に放出された原油成分から、現在天然には利用できない鯨油に代わるワックス類の生産に結びつけることを目的とした。
    このようなバイオコンバージョンを目的とする場合は分解機構の解明とそれに関わる遺伝子・酵素群に関しての情報が必要であることから、一つ一つの反応を行う酵素について性質を調べてきた。特に本研究ではアルカン分解とワックス合成との分岐点であるAcyl-CoA脱水素酵素を本菌より精製、遺伝子をクローニングし、その性質を調べた。
    本菌はAcyl-CoA脱水素酵素を少なくとも二つ保持しており、それらの遺伝子はタンデムに染色体ゲノム上に配置されていた。これらの遺伝子がコードする酵素はそれぞれ長鎖、中鎖のAcyl-CoAに活性を示し、基質特異性が異なることを明らかにした。これらはその基質特異性から、それぞれ長さの異なるアルカンに生育するときに必要な酵素であると考えられる。このように同じ反応を行う酵素でも長さの異なる基質に対応して複数の酵素を持つことは、本菌においてすでにいくつかの事例(アルカンヒドロキシラーゼ、アルコール脱水素酵素、アルデヒド脱水素酵素)で発見しており、本菌が中鎖から極長鎖までのアルカンに生育する特性を酵素レベルで明らかにしたと言える。
    今後は本遺伝子の破壊株等の構築と、それを用いたアルカンからワックス生産への収率の増大を試みる。

    researchmap

▼全件表示

 

担当授業科目

  • 応用植物環境微生物学 (2021年度) 前期  - その他

  • 植物栄養学 (2021年度) 第4学期  - 水1,水2

  • 植物環境微生物学演習 (2021年度) 前期  - その他

  • 植物環境微生物学演習 (2021年度) 前期  - その他

  • 植物環境微生物学演習 (2021年度) 後期  - その他

  • 植物環境微生物学演習 (2021年度) 後期  - その他

  • 植物-ウイルス/細菌相互作用学 (2021年度) 前期  - 木9~12

  • 生物資源科学特別研究 (2021年度) 通年  - その他

  • 応用植物環境微生物学 (2020年度) 前期  - その他

  • 植物環境微生物学演習 (2020年度) 前期  - その他

  • 植物環境微生物学演習 (2020年度) 後期  - その他

  • 植物環境微生物学演習 (2020年度) 後期  - その他

  • 植物環境微生物学演習 (2020年度) 前期  - その他

  • 植物-ウイルス/細菌相互作用 (2020年度) 前期  - その他

  • 生物資源科学特別研究 (2020年度) 通年  - その他

▼全件表示

 

社会貢献活動

  • 倉敷工業高校のスーパーエンバイロンメントスクール研究開発事業の助言

    役割:講師, 助言・指導

    倉敷工業高校  2019年4月1日 - 2022年3月31日

     詳細を見る

    種別:研究指導

    researchmap

学術貢献活動

  • Frontiers in microbiology

    役割:査読

    2021年11月18日

     詳細を見る

    種別:査読等 

    researchmap

  • Environmental microbiology

    役割:査読

    2021年10月20日

     詳細を見る

    種別:査読等 

    researchmap

  • Biology (MDPI)

    役割:査読

    2021年10月4日

     詳細を見る

    種別:査読等 

    researchmap

  • JGAM

    役割:査読

    2021年9月10日

     詳細を見る

    種別:査読等 

    researchmap

  • BBB

    役割:査読

    2021年7月3日

     詳細を見る

    種別:査読等 

    researchmap

  • Food Science and Nutrition

    役割:査読

    2021年5月15日

     詳細を見る

    種別:査読等 

    researchmap

  • Environmental microbiology

    役割:査読

    2021年3月29日

     詳細を見る

    種別:査読等 

    researchmap

  • Microbes and Environments

    役割:査読

    2021年3月18日

     詳細を見る

    種別:査読等 

    researchmap

  • Int J Mol Sci

    役割:査読

    2021年2月27日

     詳細を見る

    種別:査読等 

    researchmap

  • Journal of General and Applied Microbiology

    役割:査読

    2021年2月10日

     詳細を見る

    種別:査読等 

    researchmap

  • Microbes and Environments

    役割:査読

    2021年2月9日

     詳細を見る

    種別:査読等 

    researchmap

  • Microbes and Environments

    役割:査読

    2020年11月21日

     詳細を見る

    種別:査読等 

    researchmap

  • Plants

    役割:査読

    2020年7月23日

     詳細を見る

    種別:査読等 

    researchmap

  • Microbes and Environments

    役割:査読

    2020年7月2日

     詳細を見る

    種別:査読等 

    researchmap

  • IJMS international journal of molecular sciences

    役割:査読

    2020年6月27日

     詳細を見る

    種別:査読等 

    researchmap

  • BBB

    役割:査読

    2020年5月1日

     詳細を見る

    種別:査読等 

    researchmap

  • Biomolecules

    役割:査読

    2020年4月3日

     詳細を見る

    種別:査読等 

    researchmap

  • BBB

    役割:査読

    2020年3月25日

     詳細を見る

    種別:査読等 

    researchmap

  • Biomolecules

    役割:査読

    2020年3月19日

     詳細を見る

    種別:査読等 

    researchmap

  • BBB

    役割:査読

    2020年3月5日

     詳細を見る

    種別:査読等 

    researchmap

  • BBB

    役割:査読

    2019年12月17日

     詳細を見る

    種別:査読等 

    researchmap

  • BBB

    役割:査読

    2019年11月20日

     詳細を見る

    種別:査読等 

    researchmap

  • Microbes and Environments

    役割:査読

    2019年11月6日

     詳細を見る

    種別:査読等 

    researchmap

  • Microbes and Environments

    役割:査読

    2019年10月21日

     詳細を見る

    種別:査読等 

    researchmap

  • Environmental microbiology rep

    役割:査読

    2019年10月16日

     詳細を見る

    種別:査読等 

    researchmap

  • JGAM

    役割:査読

    2019年10月12日

     詳細を見る

    種別:査読等 

    researchmap

  • Archives of microbiology

    役割:査読

    2019年10月1日

     詳細を見る

    種別:査読等 

    researchmap

  • Microbes and environments

    役割:査読

    2019年9月2日

     詳細を見る

    種別:査読等 

    researchmap

  • Archives of microbiology

    役割:査読

    2019年7月27日

     詳細を見る

    種別:査読等 

    researchmap

  • Annals of Applied Biology

    役割:査読

    2019年7月13日

     詳細を見る

    種別:査読等 

    researchmap

  • Metabolic engineering communications

    役割:査読

    2019年5月30日

     詳細を見る

    種別:査読等 

    researchmap

  • BBB

    役割:査読

    2019年4月10日

     詳細を見る

    種別:査読等 

    researchmap

  • BBB

    役割:査読

    2019年3月24日

     詳細を見る

    種別:査読等 

    researchmap

  • Agronomy

    役割:査読

    2019年3月7日

     詳細を見る

    種別:査読等 

    researchmap

  • BBB

    役割:査読

    2019年3月2日

     詳細を見る

    種別:査読等 

    researchmap

  • IJSEM

    役割:査読

    2019年1月14日

     詳細を見る

  • JGAM

    役割:査読

    2018年11月6日

     詳細を見る

    種別:査読等 

    researchmap

  • Agronomy

    役割:査読

    2018年9月27日

     詳細を見る

    種別:査読等 

    researchmap

  • JGAM

    役割:査読

    2018年9月4日

     詳細を見る

    種別:査読等 

    researchmap

  • Plant and Soil

    役割:査読

    2018年8月20日

     詳細を見る

    種別:査読等 

    researchmap

  • Songklanakarin J Sci Tech

    役割:査読

    2018年6月21日

     詳細を見る

    種別:査読等 

    researchmap

  • J Gen Apps Microbiol

    役割:査読

    2018年6月11日

     詳細を見る

    種別:査読等 

    researchmap

  • Songklanakarin J Sci Tech

    役割:査読

    2018年6月9日

     詳細を見る

    種別:査読等 

    researchmap

  • SOJ Biochemistry Symbiosis Group

    役割:査読

    2018年5月30日

     詳細を見る

    種別:査読等 

    researchmap

  • IJSEM

    役割:査読

    2018年5月30日

     詳細を見る

    種別:査読等 

    researchmap

  • Journal of General and Applied Microbiology

    役割:査読

    2018年4月12日

     詳細を見る

    種別:査読等 

    researchmap

  • Microorganisms

    役割:査読

    2018年3月29日

     詳細を見る

    種別:査読等 

    researchmap

  • Rice

    役割:審査・評価, 査読

    2018年3月28日

     詳細を見る

    種別:査読等 

    researchmap

  • SOJ Biochemistry Symbiosis Group

    役割:査読

    2018年2月13日

     詳細を見る

    種別:査読等 

    researchmap

  • International Biodeterioration and Biodegradation

    役割:審査・評価

    2018年1月4日

     詳細を見る

    種別:査読等 

    researchmap

▼全件表示