2024/02/20 更新

写真a

ウオタニ コウジ
魚谷 弘二
Uotani Koji
所属
医歯薬学域 助教(特任)
職名
助教(特任)
外部リンク

学位

  • 博士 ( 2018年3月   岡山大学 )

研究キーワード

  • 脊椎

  • ウイルス治療

  • バイオマーカー

研究分野

  • ライフサイエンス / 整形外科学  / 脊椎脊髄外科

経歴

  • 岡山大学病院   整形外科   助教

    2021年4月 - 現在

      詳細を見る

 

論文

  • New Spinal Shortening Technique for Tethered Cord Syndrome: A Technical Note. 国際誌

    Masato Tanaka, Sumeet Sonawane, Shinya Arataki, Yoshihiro Fujiwara, Takuya Taoka, Koji Uotani, Yoshiaki Oda, Kensuke Shinohara

    Medicina (Kaunas, Lithuania)   60 ( 1 )   2023年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Background and Objectives: To present a new spinal shortening technique for tethered cord syndrome. Tethered cord syndrome (TCS) is a debilitating condition leading to progressive neurological decline. Surgical detethering for TCS is the gold standard of treatment. However, symptomatic retethering of TCS has been reported in 5%-50% of patients after initial release. To solve this problem, posterior spinal shortening osteotomy has been reported. This technique has risks of massive blood loss and neurological deterioration. The authors hereby report a new safe spinal shortening technique for tethered cord syndrome. Materials and Methods: A 31-year-old man with gait disturbance was referred to our hospital. After the delivery of treatment, he underwent surgical untethering of the spinal cord in another hospital. He had hyperreflexia of the Achilles tendon reflex and bilateral muscle weakness of the legs (MMT 3-4). He also had urinary and bowel incontinence, and total sensory loss below L5. An anteroposterior lumbar radiogram indicated partial laminectomy of L3 and L4. Lumbar MRI showed retethering of spinal cord. Results: The patient underwent a new spinal shortening technique for tethered cord syndrome under the guidance of O-arm navigation. First, from the anterior approach, disectomy from T12 to L3 was performed. Second, from the posterior approach, Ponte osteotomy was performed from T12 to L3, shortening the spinal column by 15 mm. The patient was successfully treated surgically. Postoperative lumbar MRI showed that the tension of the spinal cord was released. Manual muscle testing results and the sensory function of the left leg had recovered almost fully upon final follow-up at one year. Conclusions: A retethered spinal cord after initial untethering is difficult to treat. This new spinal shortening technique can represent another good option to release the tension of the spinal cord.

    DOI: 10.3390/medicina60010020

    PubMed

    researchmap

  • テロメラーゼ依存性腫瘍融解アデノウイルスの骨・軟部腫瘍への応用を目指した蛍光タンパクによるウイルスの治療効果予測

    魚谷 弘二, 藤原 智洋, 田澤 大, 植田 昌敬, 志渡澤 央和, 小田 孔明, 鉄永 倫子, 三澤 治夫, 中田 英二, 国定 俊之, 尾崎 敏文

    日本整形外科学会雑誌   97 ( 8 )   S1714 - S1714   2023年8月

     詳細を見る

    記述言語:日本語   出版者・発行元:(公社)日本整形外科学会  

    researchmap

  • Comparison of C-Arm-Free Oblique Lumbar Interbody Fusion L5-S1 (OLIF51) with Transforaminal Lumbar Interbody Fusion L5-S1 (TLIF51) for Adult Spinal Deformity. 国際誌

    Masato Tanaka, Sumeet Sonawane, Umesh Meena, Zhichao Lu, Yoshihiro Fujiwara, Takuya Taoka, Koji Uotani, Yoshiaki Oda, Tomoyoshi Sakaguchi, Shinya Arataki

    Medicina (Kaunas, Lithuania)   59 ( 5 )   2023年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Background and Objectives: Adult spinal deformity (ASD) surgery, L5-S1 lordosis is very important factor. The main objective of the research is to retrospectively compare symptomatic presentation and radiological presentation in the sequelae of oblique lumbar inter-body spinal fusion at L5-S1 (OLIF51) and transforaminal lumbar interbody fusion (TLIF) for ASD. Materials and Methods: We retrospectively evaluated 54 patients who underwent corrective spinal fusion for ASD between October 2019 and January 2021. Thirteen patients underwent OLIF51 (average 74.6 years old, group O) and 41 patients underwent TLIF51 (average 70.5 years old, group T). Mean follow-up period was 23.9 months for group O and 28.9 months for group T, ranging from 12 to 43 months. Clinical and radiographic outcomes are assessed using values including visual analogue scale (VAS) for back pain and Oswestry disability index (ODI). Radiographic evaluation was also collected preoperatively and at 6, 12, and 24 months postoperatively. Results: Surgical time in group O was less than that in group T (356 min vs. 492 min, p = 0.003). However, intraoperative blood loss of both groups were not significantly different (1016 mL vs. 1252 mL, p = 0.274). Changes in VAS and ODI were similar in both groups. L5-S1 angle gain and L5-S1 height gain in group O were significantly better than those of group T (9.4° vs. 1.6°, p = 0.0001, 4.2 mm vs. 0.8 mm, p = 0.0002). Conclusions: Clinical outcomes were not significantly different in both groups, but surgical time in OLIF51 was significantly less than that in TLIF51. The radiographic outcomes showed that OLIF51 created more L5-S1 lordosis and L5-S1 disc height compared with TLIF 51.

    DOI: 10.3390/medicina59050838

    PubMed

    researchmap

  • Posterolateral Floating Technique for the Thoracic Ossification of the Posterior Longitudinal Ligament with Navigation: A Technical Note.

    Masato Tanaka, Hardik Suthar, Dhvanit Desai, Taro Yamauchi, Shinya Arataki, Yoshihiro Fujiwara, Koji Uotani, Yoshiaki Oda, Haruo Misawa

    Acta medica Okayama   76 ( 6 )   743 - 748   2022年12月

     詳細を見る

    記述言語:英語  

    We describe a floating technique via a posterolateral approach with intraoperative O-arm navigation to facilitate decompression of the spinal cord in thoracic myelopathy due to severe ossification of the posterior longitudinal ligament (OPLL). A 62-year-old man with myelopathy due to thoracic OPLL had left-leg muscle weakness, urinary disturbance, and spastic gait. Bilateral leg pain and gait disturbance had persisted for 2 years. He was successfully treated by the posterolateral OPLL floating procedure and posterior pedicle fixation under O-arm navigation. At a 2-year follow-up, manual muscle testing results and sensory function of the left leg had recovered fully. His cervical Japanese Orthopedic Association score had improved from 5/12 to 11/12. The novel intraoperative O-arm navigation-guided posterolateral floating procedure for thoracic OPLL is effective for achieving precise decompression and strong fixation with a posterior approach only and can provide an excellent result for severe thoracic OPLL without the risk of adverse events from intraoperative radiation.

    DOI: 10.18926/AMO/64126

    PubMed

    researchmap

  • Three-Dimensional Analysis of the Ideal Entry Point for Sacral Alar Iliac Screws. 国際誌

    Noriyuki Watanabe, Tomoyuki Takigawa, Koji Uotani, Yoshiaki Oda, Haruo Misawa, Masato Tanaka, Toshifumi Ozaki

    Asian spine journal   16 ( 6 )   874 - 881   2022年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    STUDY DESIGN: This is a virtual three-dimensional (3D) imaging study examining computed tomography (CT) data to investigate instrumentation placement. PURPOSE: In this study, we aim to clarify the ideal entry point and trajectory of the sacral alar iliac (SAI) screw in relationship to the dorsal foramen at S1 and the respective nerve root. OVERVIEW OF LITERATURE: To the best of our knowledge, there is yet no detailed 3D imaging study on the ideal entry point of the SAI screw. Despite the evidence suggesting that the dorsal foramen at S1 is a landmark on the sacrum, the S1 nerve root disruption is a general concern during the insertion of SAI screws. No other study has been published examining the nerve root location at the S1and SAI screw insertions. METHODS: Preoperative CT data from 26 patients pertaining to adult spinal deformities were investigated in this study. We applied a 3D image processing method for a detailed investigation. Virtual cylinders were used to mimic SAI screws. These were placed to penetrate the sacral iliac joint without violating the other cortex. We then assessed the trajectory of the longest SAI screw and the ideal entry point of SAI using a color mapping method on the surface of the sacrum. We measured the location of the nerve root at S1 in relation to the foramen at S1 and the sacral surface. RESULTS: As per the results of our color mapping, it was determined that areas that received high scores are located medially and caudally to the dorsal foramen of S1. The mean angle between a horizontal line and a line connecting the medial edge of the foramen and nerve root at S1 was 93.5°. The mean distances from the dorsal medial edge of the foramen and sacral surface to S1 nerve root were 21.8 mm and 13.9 mm, respectively. CONCLUSIONS: The ideal entry point of the SAI screw is located medially and caudally to the S1 dorsal foramen based on 3D digital mapping. It is also shown that this entry point spares the S1 nerve root from possible iatrogenic injuries.

    DOI: 10.31616/asj.2021.0268

    PubMed

    researchmap

  • Transtubular Endoscopic Posterolateral Decompression for L5-S1 Lumbar Lateral Disc Herniation. 国際誌

    Masato Tanaka, Shinya Arataki, Rahul Mehta, Tsung-Ting Tsai, Yoshihiro Fujiwara, Koji Uotani, Taro Yamauchi

    Journal of visualized experiments : JoVE   ( 188 )   2022年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We report a novel technique for C-arm free transtubular L5 nerve decompression under CT-based navigation to reduce the radiation hazard. This procedure is performed under general anesthesia and neuromonitoring. The patient is placed in a prone position on an operating carbon table. A navigation reference frame is placed percutaneously into the contralateral sacroiliac joint or spinous process. Then, CT scan images are obtained. After instrument registration, the L5-S1 foraminal level is confirmed with a navigated probe, and the entry point is marked. Using an approximately 2 cm skin incision, the subcutaneous tissue and muscles are dissected. The navigated first dilator is aimed at the L5-S1 Kambin's triangle, and sequential dilation is performed. The 18 mm tube is used and fixed to the frame. The bone around the Kambin's triangle is removed with a navigated burr. For lateral disc herniation, the L5 nerve root is identified and retracted, and the disc fragment is removed. The navigation-guided tubular endoscopic decompression is an effective procedure. There is no radiation hazard to the surgeon or the operating room staff.

    DOI: 10.3791/63603

    PubMed

    researchmap

  • C-arm-Free Simultaneous OLIF51 and Percutaneous Pedicle Screw Fixation in a Single Lateral Position. 国際誌

    Masato Tanaka, Sameer Ruparel, Yoshiaki Oda, Yoshihiro Fujiwara, Sneha Shama, Koji Uotani, Shinya Arataki, Taro Yamauchi, Naveen Sake

    Journal of visualized experiments : JoVE   ( 187 )   2022年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Oblique lumbar interbody fusion (OLIF) is an established technique for the indirect decompression of lumbar canal stenosis. However, OLIF at the L5-S1 level (OLIF51) is technically difficult because of the anatomical structures. We present a novel simultaneous technique of OLIF51 with percutaneous pedicle screw fixation without fluoroscopy. The patient is placed in a right lateral decubitus position. A percutaneous reference pin is inserted into the right sacroiliac joint. An O-arm scan is performed, and 3D reconstructed images are transmitted to the spinal navigation system. A 4 cm oblique skin incision is made under navigation guidance along the pelvis. The internal/external and transverse abdominal muscles are divided along the muscle fibers, protecting the iliohypogastric and ilioinguinal nerves. Using a retroperitoneal approach, the left common iliac vessels are identified. Special muscle retractors with illumination are used to expose the L5-S1 intervertebral disc. After disc preparation with navigated instruments, the disc space is distracted with navigated trials. Autogenous bone and demineralized bone material are then inserted into the cage hole. The OLIF51 cage is inserted into the disc space with the help of a mallet. Simultaneously, percutaneous pedicle screws are inserted by another surgeon without changing the lateral decubitus position of the patient. In conclusion, C-arm-free OLIF51 and simultaneous percutaneous pedicle screw fixation are performed in a lateral position under navigation guidance. This novel technique reduces surgical time and radiation hazards.

    DOI: 10.3791/63572

    PubMed

    researchmap

  • Revision for cage migration after transforaminal/posterior lumbar interbody fusion: how to perform revision surgery? 国際誌

    Masato Tanaka, Zhang Wei, Akihiro Kanamaru, Shin Masuda, Yoshihiro Fujiwara, Koji Uotani, Shinya Arataki, Taro Yamauchi

    BMC surgery   22 ( 1 )   172 - 172   2022年5月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Symptomatic pseudarthrosis and cage migration/protrusion are difficult complications of transforaminal or posterior lumbar interbody fusion (TLIF/PLIF). If the patient experiences severe radicular symptoms due to cage protrusion, removal of the migrated cage is necessary. However, this procedure is sometimes very challenging because epidural adhesions and fibrous union can be present between the cage and vertebrae. We describe a novel classification and technique utilizing a navigated osteotome and the oblique lumbar interbody fusion at L5/S1 (OLIF51) technique to address this problem. METHODS: This retrospective study investigated consecutive patients with degenerative lumbar diseases who underwent TLIF/PLIF. Symptomatic cage migration was evaluated by direct examination, radiography, and/or computed tomography (CT) at 1, 3, 6, 12, and 24 months of follow-up. Cage migration/protrusion was defined as symptomatic cage protrusion > 5 mm from the posterior border of the over and underlying vertebral body compared with initial CT. We evaluated patient characteristics including body mass index, smoking history, fusion level, and cage type. A total of 113 patients underwent PLIF/TLIF (PLIF n = 30, TLIF n = 83), with a mean age of 71.1 years (range, 28-87 years). Mean duration of follow-up was 25 months (range, 12-47 months). RESULTS: Cage migration was identified in 5 of 113 patients (4.4%). All cases of symptomatic cage migration involved the L5/S1 level and the TLIF procedure. Risk factors for cage protrusion were age (younger), sex (male), and level (L5/S1). The mean duration to onset of cage protrusion was 3.2 months (range, 2-6 months). We applied a new classification for cage protrusion: type 1, only low back pain without new radicular symptoms; type 2, low back pain with minor radicular symptoms; or type 3, cauda equina syndrome and/or severe radicular symptoms. According to our classification, one patient was in type 1, three patients were in type 2, and one patient was in type 3. For all cases of cage migration, revision surgery was performed using a navigated high-speed burr and osteotome, and the patient in group 1 underwent additional PLIF without removal of the protruding cage. Three revision surgeries (group 2) involved removal of the protruding cage and PLIF, and one revision surgery (group 3) involved anterior removal of the cage and OLIF51 fusion. CONCLUSIONS: The navigated high-speed burr, navigated osteotome, and OLIF51 technique appear very useful for removing a cage with fibrous union from the disc in patients with pseudarthrosis. This new technique makes revision surgery after cage migration much safer, and more effective. This technique also reduces the need for fluoroscopy.

    DOI: 10.1186/s12893-022-01620-0

    PubMed

    researchmap

  • Long-Term Results of Posterior Vertebral Column Resection for Severe Thoracolumbar Kyphosis with Achondroplastic Patients: A Case Series. 国際誌

    Masato Tanaka, Tsang-Tung Chan, Haruo Misawa, Koji Uotani, Shinaya Arataki, Tomoyuki Takigawa, Tetsuro Mazaki, Yoshihisa Sugimoto

    Medicina (Kaunas, Lithuania)   58 ( 5 )   2022年4月

     詳細を見る

    記述言語:英語  

    Background and Objectives: Thoracolumbar kyphosis is one of the most frequent skeletal manifestations in patients with achondroplasia. Few papers have been published on the surgical treatment of this condition, especially in skeletally mature patients. With this study, we presented a retrospective case series of long-term surgical results for achondroplastic patients with severe thoracolumbar kyphosis. This study was conducted to evaluate the outcome of surgical treatment for thoracolumbar kyphosis in patients associated with achondroplasia presenting with paraparesis. Materials and Methods: Three patients with achondroplasia who developed neurologic deficits due to severe thoracolumbar kyphosis and underwent surgical treatment were evaluated (mean age 22.3 years; mean follow-up 9.3 years). All patients were treated with posterior vertebral column resection (p-VCR) of hypoplastic apical vertebrae with a cage and segmental instrumentation. Neurologic outcomes (JOA scores), correction of kyphosis, and operative complications were assessed. Results: All patients had back pain, neurological deficits, and urinary disturbance before surgery. The average preoperative JOA score was 8.3/11 points, which was improved to 10.7/11 points at the final follow-up (mean recovery rate 83%). All patients obtained neurologic improvement after surgery. The mean preoperative kyphotic angle was 117° (range 103°-126°). The postoperative angles averaged 37° (range 14°-57°), resulting in a mean correction rate of 67%. All patients had postoperative complications such as rod breakage and/or surgical site infection. Conclusions: The long-term results of p-VCR were acceptable for treating thoracolumbar kyphosis in patients with achondroplasia. To perform this p-VCR safely, spinal navigation and neuromonitoring are inevitable when resecting non anatomical fused vertebrae and ensuring correct pedicle screw insertion. However, surgical complications such as rod breakage and surgical site infection may occur at a high rate, making informed consent very important when surgery is indicated.

    DOI: 10.3390/medicina58050605

    PubMed

    researchmap

  • Mechanical Study of Various Pedicle Screw Systems including Percutaneous Pedicle Screw in Trauma Treatment. 国際誌

    Yoshiaki Oda, Tomoyuki Takigawa, Yasuo Ito, Haruo Misawa, Tomoko Tetsunaga, Koji Uotani, Toshifumi Ozaki

    Medicina (Kaunas, Lithuania)   58 ( 5 )   2022年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Background and Objectives: Spine surgery using a percutaneous pedicle screw placement (PPSP) is widely implemented for spinal trauma. However, percutaneous systems have been reported to have weak screw-rod connections. In this study, conventional open and percutaneous systems were biomechanically evaluated and compared. Material and Methods: The experiments were performed in two stages: the first stage was a break test, whereas the second stage was a fatigue test. Four systems were used for the experiments. System 1 was intended for conventional open surgery (titanium rod with a 6.0 mm diameter, using a clamp connecting mechanism). System 2 was a percutaneous pedicle screw (PPS) system for trauma (titanium alloy rod with a 6.0 mm diameter, using ball ring connections). System 3 was a PPS system for trauma (cobalt-chromium alloy rod with a 6.0 mm diameter, using sagittal adjusting screw connections). System 4 was a general-purpose PPS system (titanium alloy rod with a 5.5 mm diameter, using a mechanism where the adapter in the head holds down the screw). Results: Stiffness values of 54.8 N/mm, 43.1 N/mm, 90.9 N/mm, and 39.3 N/mm were reported for systems 1, 2, 3, and 4, respectively. The average number of load cycles in the fatigue test was 134,393, 40,980, 1,550,389, and 147,724 for systems 1 to 4, respectively. At the end of the test, the displacements were 0.2 mm, 16.9 mm, 1.2 mm, and 8.6 mm, respectively. System 1, with a locking mechanism, showed the least displacement at the end of the test. Conclusion: A few PPS systems showed better results in terms on stiffness and life than the open system. The experiments showed that mechanical strength varies depending on the spinal implant. The experiments conducted are essential and significant to provide the mechanical strength required for surgical reconstruction.

    DOI: 10.3390/medicina58050565

    PubMed

    researchmap

  • Comparison of Navigated Expandable Vertebral Cage with Conventional Expandable Vertebral Cage for Minimally Invasive Lumbar/Thoracolumbar Corpectomy. 国際誌

    Masato Tanaka, Mahendra Singh, Yoshihiro Fujiwara, Koji Uotani, Yoshiaki Oda, Shinya Arataki, Taro Yamauchi, Tomoyuki Takigawa, Yasuo Ito

    Medicina (Kaunas, Lithuania)   58 ( 3 )   2022年3月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Background and Objectives: The thoracolumbar burst fracture is one of the most common spinal injuries. If the patient has severe symptoms, corpectomy is indicated. Currently, minimally invasive corpectomy with a navigated expandable vertebral cage is available thanks to spinal surgical technology. The aim of this study is to retrospectively compare clinical and radiographic outcomes of conventional and navigational minimally invasive corpectomy techniques. Materials and Methods: We retrospectively evaluated 21 patients who underwent thoracolumbar minimally invasive corpectomy between October 2016 and January 2021. Eleven patients had a navigated expandable cage (group N) and 10 patients had a conventional expandable cage (group C). Mean follow-up period was 31.9 months for group N and 34.7 months for group C, ranging from 12 to 42 months in both groups. Clinical and radiographic outcomes are assessed using values including visual analogue scale (VAS) for back pain and Oswestry disability index (ODI). This data was collected preoperatively and at 6, 12, and 24 months postoperatively. Results: Surgical time and intraoperative blood loss of both groups were not significantly different (234 min vs. 267 min, 656 mL vs. 786 mL). Changes in VAS and ODI were similar in both groups. However, lateral cage mal-position ratio in group N was lower than that of group C (relative risk 1.64, Odds ratio 4.5) and postoperative cage sinking was significantly lower in group N (p = 0.033). Conclusions: Clinical outcomes are not significantly different, but radiographic outcomes of lateral cage mal-position and postoperative cage sinking were significantly lower in the navigation group.

    DOI: 10.3390/medicina58030364

    PubMed

    researchmap

  • C-arm Free O-arm Navigated Posterior Atlantoaxial Fixation in Down Syndrome: A Technical Note.

    Masato Tanaka, Sumeet Sonawane, Yoshihiro Fujiwara, Koji Uotani, Shinya Arataki, Taro Yamauchi, Youchen Ye, Haruo Misawa

    Acta medica Okayama   76 ( 1 )   71 - 78   2022年2月

     詳細を見る

    記述言語:英語  

    The surgical treatment of pediatric atlantoaxial subluxation (AAS) in Down syndrome (DS) remains technically challenging due to radiation exposure and complications such as vertebral artery injury and nonunion. The established treatment is fixation with a C1 lateral mass screw and C2 pedicle screw (modified Goel technique). However, this technique requires fluoroscopy for C1 screw insertion. To avoid exposing the operating team to radiation we present here a new C-arm free O-arm navigated surgical procedure for pediatric AAS in DS. A 5-year-old male DS patient had neck pain and unsteady gait. Radiograms showed AAS with an atlantodental interval of 10 mm, and irreducible subluxation on extension. CT scan showed Os odontoideum and AAS. MRI demonstrated spinal cord compression between the C1 posterior arch and odontoid process. We performed a C-arm free O-arm navigated modified Goel procedure with postoperative halo-vest immobilization. At oneyear follow-up, good neurological recovery and solid bone fusion were observed. The patient had no complications such as epidural hematoma, infection, or nerve or vessel injury. This novel procedure is a useful and safe technique that protects surgeons and staff from radiation risk.

    DOI: 10.18926/AMO/63214

    PubMed

    researchmap

  • Comparative Study of Bilateral Dual Sacral-Alar-Iliac Screws versus Bilateral Single Sacral-Alar-Iliac Screw for Adult Spine Deformities. 国際誌

    Koji Uotani, Masato Tanaka, Sumeet Sonawane, Sameer Ruparel, Yoshihiro Fujiwara, Shinya Arataki, Taro Yamauchi, Haruo Misawa

    World neurosurgery   156   e300-e306   2021年12月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVE: To evaluate the feasibility of O-arm navigation of bilateral dual sacral-alar-iliac (SAI) screws compared with conventional bilateral single SAI and S1 pedicle screws for pelvic anchors in cases of adult spinal deformity. METHODS: This retrospective, comparative study included 39 patients who underwent corrective fusion using SAI screws from T10 to the pelvis. Patients were divided into 2 groups according to the number of SAI screws placed during adult spinal deformity surgery: single SAI screw (group S, 17 cases) and dual SAI screws (group D, 22 cases). The incidence of rod breakage, proximal junctional kyphosis, screw loosening, reoperation, and global alignment in each group was estimated. Postoperative patient-reported outcomes were measured using the Oswestry Disability Index, Japanese Orthopaedic Association Back Pain Evaluation Questionnaire, and visual analog scale. RESULTS: The incidence of SAI screw loosening was significantly lower in group D than in group S (23% vs. 65%, P = 0.011). The rod breakage incidence was 0% and 12% in groups D and S, respectively (P = 0.17). There were no significant differences in the postoperative global alignment and clinical outcomes between the 2 groups. CONCLUSIONS: Dual SAI screws were associated with a significantly reduced incidence of screw loosening compared with single SAI screws. The bilateral dual SAI screws technique for pelvic anchors is feasible for the treatment of patients with adult spinal deformity.

    DOI: 10.1016/j.wneu.2021.09.048

    PubMed

    researchmap

  • Comparison of Simultaneous Single-Position Oblique Lumbar Interbody Fusion and Percutaneous Pedicle Screw Fixation with Posterior Lumbar Interbody Fusion Using O-arm Navigated Technique for Lumbar Degenerative Diseases. 国際誌

    Ying Tan, Masato Tanaka, Sumeet Sonawane, Koji Uotani, Yoshiaki Oda, Yoshihiro Fujiwara, Shinya Arataki, Taro Yamauchi, Tomoyuki Takigawa, Yasuo Ito

    Journal of clinical medicine   10 ( 21 )   2021年10月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Minimally invasive posterior or transforaminal lumbar interbody fusion (MI-PLIF/TLIF) are widely accepted procedures for lumbar instability due to degenerative or traumatic diseases. Oblique lateral interbody fusion (OLIF) is currently receiving considerable attention because of the reductions in damage to the back muscles and neural tissue. The aim of this study was to compare clinical and radiographic outcomes of simultaneous single-position OLIF and percutaneous pedicle screw (PPS) fixation with MI-PLIF/TLIF. This retrospective comparative study included 98 patients, comprising 63 patients with single-position OLIF (Group SO) and 35 patients with MI-PLIF/TLIF (Group P/T). Cases with more than 1 year of follow-up were included in this study. Mean follow-up was 32.9 ± 7.0 months for Group SO and 33.7 ± 7.5 months for Group P/T. Clinical and radiological evaluations were performed. Comparing Group SO to Group P/T, surgical time and blood loss were 118 versus 172 min (p < 0.01) and 139 versus 374 mL (p < 0.01), respectively. Cage height, change in disk height, and postoperative foraminal height were significantly higher in Group SO than in Group P/T. The fusion rate was 96.8% in Group SO, similar to the 94.2% in Group P/T (p = 0.985). The complication rate was 6.3% in Group SO and 14.1% in Group P/T (p = 0.191). Simultaneous single position O-arm-navigated OLIF reduces the surgical time, blood loss, and time to ambulation after surgery. Good indirect decompression can be achieved with this method.

    DOI: 10.3390/jcm10214938

    PubMed

    researchmap

  • Assessment of 3D Lumbosacral Vascular Anatomy for OLIF51 by Non-Enhanced MRI and CT Medical Image Fusion Technique. 国際誌

    Masakazu Nagamatsu, Sameer Ruparel, Masato Tanaka, Yoshihiro Fujiwara, Koji Uotani, Shinya Arataki, Taro Yamauchi, Yoshiyuki Takeshita, Rika Takamoto, Masato Tanaka, Shinsuke Moriue

    Diagnostics (Basel, Switzerland)   11 ( 10 )   2021年9月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    STUDY DESIGN: Prospective study. OBJECTIVE: Medical image fusion can provide information from multiple modalities in a single image. The present study aimed to determine whether three-dimensional (3D) lumbosacral vascular anatomy could be adequately portrayed using a non-enhanced CT-MRI medical image fusion technique. SUMMARY OF BACKGROUND DATA: Lateral lumbar interbody fusion has gained popularity for the surgical treatment of adult spinal deformity (ASD). Oblique lumbar interbody fusion at L5-S1 (OLIF51) is receiving considerable attention as a method of creating good L5-S1 lordosis. Access in OLIF51 requires evaluation of the vascular anatomy in the lumbosacral region. Conventional imaging modalities need a contrast medium to describe the vascular anatomy. METHODS: Participants comprised 15 patients with ASD or degenerative lumbar disease who underwent corrective surgery at our hospital between January 2020 and June 2021. A 3D vascular image with bony structures was obtained by fusing results from MRI and CT. We processed the merged image and measured the distance between left and right common iliac arteries and veins at two levels: the lower end of the L5 vertebral body (Window A) and the upper end of the S1 vertebral body (Window B). RESULTS: The mean sizes of Window A and Window B were 29.7 ± 10.7 mm and 36.9 ± 10.3 mm, respectively. The mean distance from the bifurcation to the lower end of the L5 vertebra was 23.7 ± 10.9 mm. Coronal deviation of the bifurcation was, from center to left, 12.6 ± 12.3 mm, and the distance from the center of the L5 vertebral body to the bifurcation was 0.79 ± 7.3 mm. Only one case showed a median sacral vein (6.7%). Clinically, we performed OLIF51 in 12 of the 15 cases (80%). CONCLUSION: Evaluating 3D lumbosacral vascular anatomy using a non-enhanced MRI and CT medical image fusion technique is very useful for OLIF51, particularly for patients in whom the use of contrast medium is contraindicated.

    DOI: 10.3390/diagnostics11101744

    PubMed

    researchmap

  • Minimally Invasive Thoracolumbar Corpectomy and Percutaneous Pedicle Screw Fixation with Computer-Assisted Rod-Bending System in Single Lateral Position: Technical Note. 国際誌

    Masato Tanaka, Mahendra Singh, Yoshihiro Fujiwara, Koji Uotani, Shinya Arataki, Taro Yamauchi, Kazuo Nakanishi

    World neurosurgery   151   138 - 144   2021年7月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Thoracolumbar corpectomy and percutaneous pedicle screw (PPS) fixation is becoming the standard method for correcting and stabilizing malalignment of spine, as is often seen in osteoporotic vertebral fracture. Nowadays, this procedure can be performed in a single lateral position with navigation. For an osteoporotic spine, accurate rod bending is necessary to prevent screw back-out. We describe a new technique using the spinal rod-bending system in a single lateral position. METHODS: A 71-year-old woman presented with severe back pain and impending paraplegia secondary to L1 osteoporotic vertebral fracture. We performed minimally invasive L1 corpectomy with an expandable vertebral cage and short-segment PPS with computer-assisted rod bending in a single lateral position under navigation guidance. RESULTS: The patient was successfully treated with surgery, and her low back pain improved. Her clinical outcomes improved; the Oswestry Disability Index went from 54% to 26%, and her low back pain visual analog scale score went from 78 mm to 19 mm at the 2-year final follow-up. CONCLUSIONS: Minimally invasive surgery thoracolumbar corpectomy using a computer-assisted spinal rod-bending system is a valuable technique to reduce screw back-out for osteoporotic vertebrae. With this new technique, the rod bending becomes easy, even for long PPS fusion with the severe osteoporotic or deformity patient in a single lateral position.

    DOI: 10.1016/j.wneu.2021.05.028

    PubMed

    researchmap

  • Navigated Lateral Osteotomy for Adult Spinal Deformity: A Technical Note. 国際誌

    Masato Tanaka, Koji Uotani, Yoshihiro Fujiwara, Kentaro Yamane, Sumeet Sonawane, Shinya Arataki, Taro Yamauchi

    World neurosurgery   150   56 - 63   2021年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Minimally invasive surgery is receiving considerable attention as a technique for reducing the complications of adult spinal deformity (ASD) surgery. For this technique, a new lateral osteotomy plays an important role to release fused vertebrae. We describe herein a novel navigated lateral osteotomy technique not requiring C-arm fluoroscopy to correct adult spinal deformities. CASE DESCRIPTION: A 68-year-old woman with symptomatic ASD and a 4-year history of severe low back pain affecting daily life was referred to our hospital. Surgery was performed without C-arm fluoroscopy. A navigated osteotome was used to release the fused L1/2 mass. The patient was successfully treated with surgery, and low back pain was well controlled. In terms of clinical outcomes, Oswestry Disability Index improved from 64%-19% and Visual Analog Scale score for low back pain improved from 74 mm-19 mm on final follow-up at 2 years. CONCLUSIONS: This novel navigated lateral osteotomy for ASD is a useful technique that enables minimally invasive surgery for fixed deformity. With this new technique, surgeons and operating room staff can avoid adverse effects of intraoperative radiation.

    DOI: 10.1016/j.wneu.2021.03.088

    PubMed

    researchmap

  • C-Arm-Free Anterior Correction for Adolescent Idiopathic Scoliosis (Lenke Type 5C): Analysis of Early Outcomes and Complications. 国際誌

    Masato Tanaka, Yoshihiro Fujiwara, Koji Uotani, Taro Yamauchi, Hauo Misawa

    World neurosurgery   150   e561-e569   2021年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Computer-assisted spinal surgery as a technique for reducing radiation exposure to the operating staff and the complications of spinal deformity are receiving considerable attention. However, no technical reports have described the technique for navigating anterior correction of adolescent idiopathic scoliosis without C-arm fluoroscopy. The purpose of this study was to evaluate the efficacy and safety of this new C-arm-free anterior correction for scoliosis. METHODS: This study investigated 38 consecutive patients with Lenke type 5C curves who underwent selective lumbar or thoracolumbar fusion, comprising 26 patients with conventional anterior correction surgery, and 12 patients with C-arm-free navigation surgery. The 2 groups were evaluated immediately postoperatively and at the 2-year follow-up. RESULTS: No vascular injuries, screw malpositioning, or major complications were associated with the surgical procedure in either group. Correction rates of the lumbar curve were satisfactory with no significant difference between groups (mean, 82.6% ± 5.7% vs. 80.7% ± 10.2%, respectively). However, mean time for fluoroscopy in group C was 133 ± 9.5 seconds (P < 0.0001). No significant differences in intraoperative blood loss (642 ± 123 mL vs. 731 ± 222 mL, respectively) or surgical time (251 ± 13 min vs. 301 ± 38 min, respectively) were seen between groups. Mean final follow-up Scoliosis Research Society Outcomes Questionnaire (SRS-22) was also excellent for both group C (4.2 ± 0.19) and group N (4.3 ± 0.20). CONCLUSIONS: C-arm-free anterior correction offers safe, effective surgery for adolescent idiopathic scoliosis. The advantage of this new technique is no radiation exposure for medical staff at centers performing large numbers of spinal procedures.

    DOI: 10.1016/j.wneu.2021.03.060

    PubMed

    researchmap

  • Effect of an Adjustable Hinged Carbon Fiber Operating Table on the Coronal Alignment of the Lumbar Spine During Oblique Lateral Interbody Fusion. 国際誌

    Ying Tan, Masato Tanaka, Yoshihiro Fujiwara, Koji Uotani, Taro Yamauchi, Masanori Yorimitsu, Yusuke Yokoyama, Sumeet Sonawane

    World neurosurgery   149   e958-e962   2021年5月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    OBJECTIVE: We sought to measure the coronal alignment of the lumbar spine of patients in the right lateral decubitus position on a hinged Jackson operating table with the following 3 table positions: neutral and right and left 20-degree flexion. METHODS: We analyzed the data of 23 patients who underwent OLIF. Spinal alignment was quantified using the coronal Cobb angle from L1 to S1, measured on anterior-posterior radiographs obtained preoperatively, after induction of anesthesia, with patients in the right lateral decubitus position, for the following 3 positions of the Jackson hinged operating table: neutral, right 20-degree flexion, and left 20-degree flexion. The Cobb angle at each position, the change in the Cobb angle, and the effective range of motion (%) were obtained from neutral to right and left 20-degree flexion. Alignment was compared between the 3 positions, and the range of motion was compared between men and women. RESULTS: The Cobb angle was different in all 3 positions of the table (P < 0.0001): -7.0 ± 8.7°, neutral; 2.8 ± 7.6°, right 20-degree flexion; and -14.7 ± 7.8°, left 20-degree flexion. The change in Cobb angle and the effective range of motion were greater in women (10.9 ± 2.8° and 55%) than in men (6.7 ± 5.8° and 34%) from the neutral to right 20-degree flexion position (P = 0.0298). CONCLUSIONS: The coronal alignment of the lumbar spine of patients in the right lateral decubitus position on a flat operating table (neutral position) was convex. The right 20-degree flexion position of the hinged operating table yielded less coronal plane lumbar spine deformity, with greater deformity in women.

    DOI: 10.1016/j.wneu.2021.01.066

    PubMed

    researchmap

  • Liquid Biopsy Targeting Monocarboxylate Transporter 1 on the Surface Membrane of Tumor-Derived Extracellular Vesicles from Synovial Sarcoma. 国際誌

    Suguru Yokoo, Tomohiro Fujiwara, Aki Yoshida, Koji Uotani, Takuya Morita, Masahiro Kiyono, Joe Hasei, Eiji Nakata, Toshiyuki Kunisada, Shintaro Iwata, Tsukasa Yonemoto, Koji Ueda, Toshifumi Ozaki

    Cancers   13 ( 8 )   2021年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The lack of noninvasive biomarkers that can be used for tumor monitoring is a major problem for soft-tissue sarcomas. Here we describe a sensitive analytical technique for tumor monitoring by detecting circulating extracellular vesicles (EVs) of patients with synovial sarcoma (SS). The proteomic analysis of purified EVs from SYO-1, HS-SY-II, and YaFuSS identified 199 common proteins. DAVID GO analysis identified monocarboxylate transporter 1 (MCT1) as a surface marker of SS-derived EVs, which was also highly expressed in SS patient-derived EVs compared with healthy individuals. MCT1+CD9+ EVs were also detected from SS-bearing mice and their expression levels were significantly correlated with tumor volume (p = 0.003). Furthermore, serum levels of MCT1+CD9+ EVs reflected tumor burden in SS patients. Immunohistochemistry revealed that MCT1 was positive in 96.7% of SS specimens and its expression on the cytoplasm/plasma membrane was significantly associated with worse overall survival (p = 0.002). Silencing of MCT1 reduced the cellular viability, and migration and invasion capability of SS cells. This work describes a new liquid biopsy technique to sensitively monitor SS using circulating MCT1+CD9+ EVs and indicates the therapeutic potential of MCT1 in SS.

    DOI: 10.3390/cancers13081823

    PubMed

    researchmap

  • Percutaneous C-Arm Free O-Arm Navigated Biopsy for Spinal Pathologies: A Technical Note. 国際誌

    Masato Tanaka, Sumeet Sonawane, Koji Uotani, Yoshihiro Fujiwara, Kittipong Sessumpun, Taro Yamauchi, Shinsuke Sugihara

    Diagnostics (Basel, Switzerland)   11 ( 4 )   2021年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Percutaneous biopsy under computed tomography (CT) guidance is a standard technique to obtain a definitive diagnosis when spinal tumors, metastases or infections are suspected. However, specimens obtained using a needle are sometimes inadequate for correct diagnosis. This report describes a unique biopsy technique which is C-arm free O-arm navigated using microforceps. This has not been previously described as a biopsy procedure. CASE DESCRIPTION: A 74-year-old man with T1 vertebra pathology was referred to our hospital with muscle weakness of the right hand, clumsiness and cervicothoracic pain. CT-guided biopsy was performed, but histopathological diagnosis could not be obtained due to insufficient tissue. The patient then underwent biopsy under O-arm navigation, so we could obtain sufficient tissue and small cell carcinoma was diagnosed on histopathological examination. A patient later received chemotherapy and radiation. CONCLUSIONS: C-arm free O-arm navigated biopsy is an effective technique for obtaining sufficient material from spine pathologies. Tissue from an exact pathological site can be obtained with 3-D images. This new O-arm navigation biopsy may provide an alternative to repeat CT-guided or open biopsy.

    DOI: 10.3390/diagnostics11040636

    PubMed

    researchmap

  • Transtubular Endoscopic Posterolateral Decompression of the L5 Root under Navigation and O-arm: A Technical Note.

    Rahul Mehta, Masato Tanaka, Yoshiaki Oda, Yoshihiro Fujiwara, Koji Uotani, Shinya Arataki, Taro Yamauchi

    Acta medica Okayama   75 ( 5 )   637 - 640   2021年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Among studies evaluating minimally invasive surgical (MIS) decompression of the L5 root, techniques involving transtubular endoscopic decompression under O-arm navigation are rare. We present the case of a 68-yearold woman with left leg pain, muscle weakness and gait disturbance of one month duration. The patient underwent transtubular endoscopic decompression under O-arm navigation. There is no radiation hazard to the operating room staff with this procedure. After surgery, the patient had significant pain relief and her left lower limb motor function had improved by follow-up at one year. C-arm-free endoscopic L5 root decompression is a safe and effective procedure.

    DOI: 10.18926/AMO/62777

    PubMed

    researchmap

  • O-arm Navigation-Guided Surgical Resection and Posterior Fixation for a Large Sacral Schwannoma.

    Koji Uotani, Taro Yamauchi, Keisuke Sano, Hiroshi Sonobe, Yoshihiro Fujiwara, Praful Suresh Maste, Sumeet Sonawane, Masato Tanaka

    Acta medica Okayama   75 ( 5 )   647 - 652   2021年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Sacral schwannoma is a rare tumor with relatively few symptoms; it thus tends to be large at diagnosis and is challenging to treat surgically. We present the case of a 12-year-old girl with a large sacral schwannoma that was successfully surgically resected using O-arm navigation in a two-stage operation. First, we performed tumor resection from the posterior aspect with assisted O-arm navigation. One week later, resection from the anterior aspect was conducted with posterior spinopelvic fixation and fibula graft. We performed partial resection of the tumor from the anterior and posterior aspects as much as possible. O-arm navigation contributed to precise and safe tumor resection and implant insertion.

    DOI: 10.18926/AMO/62779

    PubMed

    researchmap

  • C-Arm-Free Minimally Invasive Cervical Pedicle Screw Fixation (MICEPS): A Technical Note.

    Masato Tanaka, Yoshihiro Fujiwara, Koji Uotani, Venkatesh Kadiri, Taro Yamauchi

    Acta medica Okayama   74 ( 6 )   551 - 556   2020年12月

     詳細を見る

    記述言語:英語  

    A minimally invasive posterolateral approach designed to avoid the lateral misplacement of midcervical pedicle screws was reported, but there is no technical report that describes this technique without C-arm fluoroscopy. We report the results of a 2.5 years follow-up of a 62-year-old female patient with C4 metastatic breast cancer. The patient suffered from severe neck pain and impending quadriplegia for 2 months after radiation therapy. We performed C-arm-free minimally invasive cervical pedicle screw fixation (MICEPS). The patient was suc-cessfully treated with surgery, and her neck pain was well controlled. She had neither neurological deficits nor neck pain at the final (2.5-year) follow-up. C-arm-free MICEPS is a useful technique; in addition, the sur-geons and staff have no risk of radiation exposure, there is a reduced need for postoperative imaging, and a decreased revision rate can be expected with C-arm-free MICEPS.

    DOI: 10.18926/AMO/61216

    PubMed

    researchmap

  • C-Arm-Free Circumferential Minimally Invasive Surgery for Adult Spinal Deformity: Technical Note. 国際誌

    Masato Tanaka, Yoshihiro Fujiwara, Koji Uotani, Praful Maste, Taro Yamauchi

    World neurosurgery   143   235 - 246   2020年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Circumferential minimally invasive surgery (cMIS), as a technique for reducing the complications of adult spinal deformity surgery, is receiving considerable attention. Conventional cMIS is performed using intraoperative fluoroscopy. In this work, we describe a new cMIS technique without using C-arm fluoroscopy for correcting adult spinal deformities. CASE DESCRIPTION: A 70-year-old woman with symptomatic adult spinal deformity was referred to our hospital. The patient's daily life has been affected by severe low back pain for more than 4 years. The surgery was performed without C-arm fluoroscopy. The percutaneous pedicle screws and intervertebral cages were inserted only under navigation guidance. The patient was successfully treated with surgery, and her low back pain was well controlled. Her clinical outcomes were as follows: Oswestry Disability Index improved from 64% to 14% and the low back pain visual analog scale score improved from 75 mm to 24 mm at 1 year of final follow-up. CONCLUSIONS: C-arm-free cMIS for adult spinal deformity is a useful technique that reduces percutaneous screw and cage misplacement. With this new technique, the surgeons and the operation room staff can avoid the risk of an adverse event of intraoperative radiation.

    DOI: 10.1016/j.wneu.2020.07.033

    PubMed

    researchmap

  • Clinical relevance and functional significance of cell-free microRNA-1260b expression profiles in infiltrative myxofibrosarcoma. 国際誌

    Takuya Morita, Tomohiro Fujiwara, Aki Yoshida, Koji Uotani, Masahiro Kiyono, Suguru Yokoo, Joe Hasei, Toshiyuki Kunisada, Toshifumi Ozaki

    Scientific reports   10 ( 1 )   9414 - 9414   2020年6月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Infiltrative tumor growth into adjacent soft tissues is a major cause of the frequent recurrence and tumor-related death of myxofibrosarcoma (MFS), but no useful biomarkers reflecting tumor burden and infiltrative growth are available. While emerging evidence suggests a diagnostic and functional role of extracellular/circulating microRNA (miRNA) in various malignant diseases, their significance in MFS patients remains unknown. Global miRNA profiling identified four upregulated miRNAs in MFS patient sera and culture media of MFS cells. Among these, serum miR-1260b level was significantly upregulated in patient serum discriminating from healthy individuals and closely correlated with clinical status and tumor dynamics in MFS-bearing mice. In addition, high miR-1260b expression in serum was correlated with radiological tail-like patterns, characteristic of the infiltrative MFS. The extracellular miR-1260b was embedded in tumor-derived extracellular vesicles (EVs) and promoted cellular invasion of MFS through the downregulation of PCDH9 in the adjacent normal fibroblasts. Collectively, circulating miR-1260b expression may represent a novel diagnostic target for tumor monitoring of this highly aggressive sarcoma. Moreover, EV-miR-1260b could act as a transfer messenger to adjacent cells and mediate the infiltrative growth of MFS, providing new insights into the mechanism of infiltrative nature via crosstalk between tumor cells and their microenvironment.

    DOI: 10.1038/s41598-020-66120-8

    PubMed

    researchmap

  • Clinical and Functional Significance of Intracellular and Extracellular microRNA-25-3p in Osteosarcoma.

    Aki Yoshida, Tomohiro Fujiwara, Koji Uotani, Takuya Morita, Masahiro Kiyono, Suguru Yokoo, Joe Hasei, Eiji Nakata, Toshiyuki Kunisada, Toshifumi Ozaki

    Acta medica Okayama   72 ( 2 )   165 - 174   2018年4月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Although there is considerable evidence indicating that the dysregulation of microRNAs (miRNAs) in malignant tumors plays a role in tumor development, the overall function of miRNAs and their clinicopathological significance are not well understood. In this retrospective analysis of 45 biopsy specimens from osteosarcoma (OS) patients, we investigated the functional and clinical significance of miR-25-3p in OS, which we previously identified as a highly expressed miRNA in OS patients' serum. We observed that miR-25-3p dysregulation in human OS tissues was negatively correlated with the clinical prognosis, whereas the expression level of its target gene, Dickkopf WNT Signaling Pathway Inhibitor 3 (DKK3), was positively correlated with the clinical prognosis. Endogenous miR-25-3p upregulation promoted tumor growth, invasion, and drug resistance, which was consistent with DKK3 silencing in OS cells. In addition, secretory miR-25-3p was embedded in tumor-derived exosomes, where it promoted capillary formation and the invasion of vascular endothelial cells. Overall, our results show that miR-25-3p has intracellular and extracellular oncogenic functions as well as clinicopathological relevance in OS, indicating its potential as a novel diagnostic and therapeutic tool for the clinical management of this disease.

    DOI: 10.18926/AMO/55857

    PubMed

    researchmap

  • Circulating MicroRNA-92b-3p as a Novel Biomarker for Monitoring of Synovial Sarcoma. 国際誌

    Koji Uotani, Tomohiro Fujiwara, Aki Yoshida, Shintaro Iwata, Takuya Morita, Masahiro Kiyono, Suguru Yokoo, Toshiyuki Kunisada, Ken Takeda, Joe Hasei, Kunihiko Numoto, Yutaka Nezu, Tsukasa Yonemoto, Takeshi Ishii, Akira Kawai, Takahiro Ochiya, Toshifumi Ozaki

    Scientific reports   7 ( 1 )   14634 - 14634   2017年11月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The lack of useful biomarkers is a crucial problem for patients with soft tissue sarcomas (STSs). Emerging evidence has suggested that circulating microRNAs (miRNAs) in body fluids have novel impact as biomarkers for patients with malignant diseases, but their significance in synovial sarcoma (SS) patients remains unknown. Initial global miRNA screening using SS patient serum and SS cell culture media identified a signature of four upregulated miRNAs. Among these candidates, miR-92b-3p secretion from SS cells was confirmed, which was embedded within tumour-derived exosomes rather than argonaute-2. Animal experiments revealed a close correlation between serum miR-92b-3p levels and tumour dynamics. Clinical relevance was validated in two independent clinical cohorts, and we subsequently identified that serum miR-92b-3p levels were significantly higher in SS patients in comparison to that in healthy individuals. Moreover, serum miR-92b-3p was robust in discriminating patients with SS from the other STS patients and reflected tumour burden in SS patients. Overall, liquid biopsy using serum miR-92b-3p expression levels may represent a novel approach for monitoring tumour dynamics of SS.

    DOI: 10.1038/s41598-017-12660-5

    PubMed

    researchmap

  • Clinical significance of circulating miR-25-3p as a novel diagnostic and prognostic biomarker in osteosarcoma. 国際誌

    Tomohiro Fujiwara, Koji Uotani, Aki Yoshida, Takuya Morita, Yutaka Nezu, Eisuke Kobayashi, Akihiko Yoshida, Takenori Uehara, Toshinori Omori, Kazuhisa Sugiu, Tadashi Komatsubara, Ken Takeda, Toshiyuki Kunisada, Machiko Kawamura, Akira Kawai, Takahiro Ochiya, Toshifumi Ozaki

    Oncotarget   8 ( 20 )   33375 - 33392   2017年5月

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    BACKGROUND: Emerging evidence has suggested that circulating microRNAs (miRNAs) in body fluids have novel diagnostic and prognostic significance for patients with malignant diseases. The lack of useful biomarkers is a crucial problem of bone and soft tissue sarcomas; therefore, we investigated the circulating miRNA signature and its clinical relevance in osteosarcoma. METHODS: Global miRNA profiling was performed using patient serum collected from a discovery cohort of osteosarcoma patients and controls and cell culture media. The secretion of the detected miRNAs from osteosarcoma cells and clinical relevance of serum miRNA levels were evaluated using in vitro and in vivo models and a validation patient cohort. RESULTS: Discovery screening identified 236 serum miRNAs that were highly expressed in osteosarcoma patients compared with controls, and eight among these were also identified in the cell culture media. Upregulated expression levels of miR-17-5p and miR-25-3p were identified in osteosarcoma cells, and these were abundantly secreted into the culture media in tumor-derived exosomes. Serum miR-25-3p levels were significantly higher in osteosarcoma patients than in control individuals in the validation cohort, with favorable sensitivity and specificity compared with serum alkaline phosphatase. Furthermore, serum miR-25-3p levels at diagnosis were correlated with patient prognosis and reflected tumor burden in both in vivo models and patients; these associations were more sensitive than those of serum alkaline phosphatase. CONCLUSIONS: Serum-based circulating miR-25-3p may serve as a non-invasive blood-based biomarker for tumor monitoring and prognostic prediction in osteosarcoma patients.

    DOI: 10.18632/oncotarget.16498

    PubMed

    researchmap

  • 手・足部発生の類骨骨腫の治療経験

    小松原 将, 武田 健, 森田 卓也, 杉生 和久, 魚谷 弘二, 大森 敏規, 上原 健敬, 吉田 晶, 藤原 智洋, 国定 俊之, 尾崎 敏文

    日本整形外科学会雑誌   90 ( 6 )   S1396 - S1396   2016年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:(公社)日本整形外科学会  

    researchmap

  • 仙骨骨巨細胞腫に対する治療戦略

    藤原 智洋, 国定 俊之, 武田 健, 山川 泰明, 大森 敏規, 上原 健敬, 魚谷 弘二, 杉生 和久, 尾崎 敏文

    日本整形外科学会雑誌   89 ( 6 )   S1308 - S1308   2015年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:(公社)日本整形外科学会  

    researchmap

  • MicroRNAs in soft tissue sarcomas: overview of the accumulating evidence and importance as novel biomarkers. 国際誌

    Tomohiro Fujiwara, Toshiyuki Kunisada, Ken Takeda, Koji Uotani, Aki Yoshida, Takahiro Ochiya, Toshifumi Ozaki

    BioMed research international   2014   592868 - 592868   2014年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Sarcomas are distinctly heterogeneous tumors and a variety of subtypes have been described. Although several diagnostic explorations in the past three decades, such as identification of chromosomal translocation, have greatly improved the diagnosis of soft tissue sarcomas, the unsolved issues, including the limited useful biomarkers, remain. Emerging reports on miRNAs in soft tissue sarcomas have provided clues to solving these problems. Evidence of circulating miRNAs in patients with soft tissue sarcomas and healthy individuals has been accumulated and is accelerating their potential to develop into clinical applications. Moreover, miRNAs that function as novel prognostic factors have been identified, thereby facilitating their use in miRNA-targeted therapy. In this review, we provide an overview of the current knowledge on miRNA deregulation in soft tissue sarcomas, and discuss their potential as novel biomarkers and therapeutics.

    DOI: 10.1155/2014/592868

    PubMed

    researchmap

▼全件表示

MISC

  • 骨肉腫における細胞内外の単一microRNAの機能について(Clinical and Functional Significance of Single Intracellular and Extracellular Onco-microRNA in Osteosarcoma)

    吉田 晶, 藤原 智洋, 魚谷 弘二, 森田 卓也, 清野 正普, 横尾 賢, 長谷井 嬢, 国定 俊之, 尾崎 敏文

    日本癌学会総会記事   77回   2253 - 2253   2018年9月

     詳細を見る

    記述言語:英語   出版者・発行元:(一社)日本癌学会  

    researchmap

  • 腫瘍融解ウイルスの骨・軟部腫瘍組織に対する感染効率の評価

    吉田 晶, 魚谷 弘二, 藤原 智洋, 長谷井 嬢, 森田 卓也, 清野 正普, 杉生 和久, 小松原 将, 望月 雄介, 国定 俊之, 藤原 俊義, 尾崎 敏文

    日本整形外科学会雑誌   91 ( 8 )   S1823 - S1823   2017年8月

     詳細を見る

    記述言語:日本語   出版者・発行元:(公社)日本整形外科学会  

    researchmap

  • 悪性骨・軟部腫瘍転移制御への道 粘液線維肉腫における分泌型microRNAは腫瘍の浸潤性と関連しているか

    森田 卓也, 藤原 智洋, 魚谷 弘二, 吉田 晶, 清野 正普, 杉生 和久, 小松原 将, 望月 雄介, 長谷井 嬢, 国定 俊之, 尾崎 敏文

    日本整形外科学会雑誌   91 ( 8 )   S1709 - S1709   2017年8月

     詳細を見る

    記述言語:日本語   出版者・発行元:(公社)日本整形外科学会  

    researchmap

  • 骨肉腫細胞内と分泌エクソソーム内におけるmicroRNA発現プロファイリングの相違と患者循環エクソソーム内の発現評価

    清野 正普, 藤原 智洋, 魚谷 弘二, 吉田 晶, 森田 卓也, 杉生 和久, 小松原 将, 望月 雄介, 長谷井 嬢, 国定 俊之, 尾崎 敏文

    日本整形外科学会雑誌   91 ( 8 )   S1525 - S1525   2017年8月

     詳細を見る

    記述言語:日本語   出版者・発行元:(公社)日本整形外科学会  

    researchmap

  • 骨肉腫細胞から分泌されるエクソソームは腫瘍進展においてどのような役割を担っているか

    清野 正普, 藤原 智洋, 魚谷 弘二, 吉田 晶, 森田 卓也, 杉生 和久, 小松原 将, 望月 雄介, 長谷井 嬢, 国定 俊之, 尾崎 敏文

    日本整形外科学会雑誌   91 ( 6 )   S1320 - S1320   2017年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:(公社)日本整形外科学会  

    researchmap

  • 滑膜肉腫における分泌型microRNAの細胞外安定性の解析

    魚谷 弘二, 藤原 智洋, 吉田 晶, 森田 卓也, 清野 正普, 長谷井 嬢, 国定 俊之, 根津 悠, 岩田 慎太郎, 川井 章, 落谷 孝広, 尾崎 敏文

    日本整形外科学会雑誌   91 ( 6 )   S1355 - S1355   2017年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:(公社)日本整形外科学会  

    researchmap

  • 骨・軟部肉腫切除術における感染リスクファクターの検討

    長谷井 嬢, 望月 雄介, 小松原 将, 国定 俊之, 藤原 智洋, 魚谷 弘二, 杉生 和久, 尾崎 敏文

    日本整形外科学会雑誌   91 ( 2 )   S598 - S598   2017年3月

     詳細を見る

    記述言語:日本語   出版者・発行元:(公社)日本整形外科学会  

    researchmap

  • 骨肉腫細胞内と細胞外分泌エクソソーム内のmicroRNA発現プロファイリングは転移能の違いによりどのように異なるか

    清野 正普, 藤原 智洋, 魚谷 弘二, 吉田 晶, 杉生 和久, 小松原 将, 望月 雄介, 長谷井 嬢, 國定 俊之, 尾崎 敏文

    日本癌学会総会記事   75回   P - 3085   2016年10月

     詳細を見る

    記述言語:英語   出版者・発行元:(一社)日本癌学会  

    researchmap

  • 骨肉腫細胞内と細胞外分泌エクソソーム内のmicroRNA発現プロファイリングはどのように異なるか

    清野 正普, 藤原 智洋, 魚谷 弘二, 吉田 晶, 森田 卓也, 杉生 和久, 小松原 将, 望月 雄介, 国定 俊之, 尾崎 敏文

    日本整形外科学会雑誌   90 ( 8 )   S1801 - S1801   2016年8月

     詳細を見る

    記述言語:日本語   出版者・発行元:(公社)日本整形外科学会  

    researchmap

  • 臨床検体を用いた腫瘍融解アデノウイルスの骨・軟部腫瘍への適応に関する検討

    吉田 晶, 魚谷 弘二, 藤原 智洋, 長谷井 嬢, 大森 敏規, 杉生 和久, 小松原 将, 森田 卓也, 清野 正晋, 望月 雄介, 武田 健, 国定 俊之, 藤原 俊義, 尾崎 敏文

    日本整形外科学会雑誌   90 ( 8 )   S1627 - S1627   2016年8月

     詳細を見る

    記述言語:日本語   出版者・発行元:(公社)日本整形外科学会  

    researchmap

  • 軟部肉腫に対する腫瘍融解アデノウイルスと放射線治療の併用効果の検討

    小松原 将, 大森 敏規, 杉生 和久, 森田 卓也, 魚谷 弘二, 吉田 晶, 藤原 智洋, 武田 健, 国定 俊之, 浦田 泰生, 尾崎 敏文

    日本整形外科学会雑誌   90 ( 8 )   S1565 - S1565   2016年8月

     詳細を見る

    記述言語:日本語   出版者・発行元:(公社)日本整形外科学会  

    researchmap

  • Identification of circulating tumor derived microRNA signatures in osteosarcoma

    Tomohiro Fujiwara, Koji Uotani, Aki Yoshida, Takuya Morita, Tadashi Komatsubara, Kazuhisa Sugiu, Toshinori Oman, Ken Takeda, Toshiyuki Kunisada, Yutaka Nezu, Akira Kawai, Hirotaka Kanzaki, Takahiro Ochiya, Toshifumi Ozaki

    CANCER RESEARCH   76   2016年7月

     詳細を見る

    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:AMER ASSOC CANCER RESEARCH  

    Web of Science

    researchmap

  • 骨軟部肉腫切除における感染リスクファクター

    望月 雄介, 小松原 将, 長谷井 嬢, 国定 俊之, 藤原 智洋, 魚谷 弘二, 杉生 和久, 尾崎 敏文

    日本骨・関節感染症学会プログラム・抄録集   39回   90 - 90   2016年7月

     詳細を見る

    記述言語:日本語   出版者・発行元:(一社)日本骨・関節感染症学会  

    researchmap

  • 術中ナビゲーションが有用であった脛骨原発phosphaturic mesenchymal tumorの1例

    藤原 智洋, 国定 俊之, 武田 健, 上原 健敬, 大森 敏規, 魚谷 弘二, 杉生 和久, 森田 卓也, 小松原 将, 吉田 晶, 西山 悠紀, 稲垣 謙一, 尾崎 敏文

    日本整形外科学会雑誌   90 ( 6 )   S1398 - S1398   2016年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:(公社)日本整形外科学会  

    researchmap

  • 脊椎に発生したpigmented villonodular synovitisの2例

    魚谷 弘二, 藤原 智洋, 瀧川 朋亨, 荒瀧 慎也, 杉本 佳久, 武田 健, 国定 俊之, 田中 雅人, 尾崎 敏文

    日本整形外科学会雑誌   90 ( 6 )   S1353 - S1353   2016年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:(公社)日本整形外科学会  

    researchmap

  • 軟部原発平滑筋肉腫の治療成績

    藤原 智洋, 国定 俊之, 武田 健, 上原 健敬, 大森 敏規, 魚谷 弘二, 杉生 和久, 森田 卓也, 小松原 将, 吉田 晶, 尾崎 敏文

    日本整形外科学会雑誌   90 ( 6 )   S1308 - S1308   2016年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:(公社)日本整形外科学会  

    researchmap

  • 胞巣状軟部肉腫の特徴的な画像所見

    国定 俊之, 武田 健, 藤原 智洋, 魚谷 弘二, 杉生 和久, 尾崎 敏文

    中部日本整形外科災害外科学会雑誌   59 ( 春季学会 )   89 - 89   2016年3月

     詳細を見る

    記述言語:日本語   出版者・発行元:(一社)中部日本整形外科災害外科学会  

    researchmap

  • 骨・軟部肉腫切除後にプレート固定した自家処理骨移植の治療成績

    武田 健, 国定 俊之, 森田 卓也, 小松原 将, 魚谷 弘二, 杉生 和久, 藤原 智洋, 杉原 進介, 尾崎 敏文

    日本整形外科学会雑誌   90 ( 2 )   S171 - S171   2016年3月

     詳細を見る

    記述言語:日本語   出版者・発行元:(公社)日本整形外科学会  

    researchmap

  • 骨軟部肉腫細胞に対する腫瘍融解ウイルスの放射線増感作用

    大森 敏規, 山川 泰明, 長谷井 嬢, 田澤 大, 尾崎 修平, 佐々木 剛, 杉生 和久, 魚谷 弘二, 藤原 智洋, 国定 俊之, 浦田 泰生, 尾崎 敏文, 藤原 俊義

    日本癌学会総会記事   74回   E - 1276   2015年10月

     詳細を見る

    記述言語:英語   出版者・発行元:(一社)日本癌学会  

    researchmap

  • 腫瘍特異的増殖型ウイルスによる骨・軟部肉腫circulating tumor cell(CTC)検出法の検討

    小松原 将, 山川 泰明, 大森 敏規, 杉生 和久, 魚谷 弘二, 吉田 晶, 藤原 智洋, 武田 健, 国定 俊之, 浦田 泰生, 尾崎 敏文

    日本整形外科学会雑誌   89 ( 8 )   S1795 - S1795   2015年9月

     詳細を見る

    記述言語:日本語   出版者・発行元:(公社)日本整形外科学会  

    researchmap

  • 骨盤発生の放射線照射後肉腫の治療成績

    武田 健, 国定 俊之, 藤原 智洋, 魚谷 弘二, 杉生 和久, 尾崎 敏文

    中部日本整形外科災害外科学会雑誌   58 ( 秋季学会 )   286 - 286   2015年9月

     詳細を見る

    記述言語:日本語   出版者・発行元:(一社)中部日本整形外科災害外科学会  

    researchmap

  • 骨肉腫におけるmyeloid derived suppressor cell(MDSC)に対するmetforminの作用の検討

    上原 健敬, 榮川 伸吾, 藤原 智洋, 杉生 和久, 魚谷 弘二, 大森 敏規, 吉田 晶, 武田 健, 国定 俊之, 鵜殿 平一郎, 尾崎 敏文

    日本整形外科学会雑誌   89 ( 8 )   S1670 - S1670   2015年9月

     詳細を見る

    記述言語:日本語   出版者・発行元:(公社)日本整形外科学会  

    researchmap

  • 滑膜肉腫患者血清から特定した滑膜肉腫特異的分泌型miRNAに関する検討

    魚谷 弘二, 藤原 智洋, 吉田 晶, 山川 泰明, 大森 敏規, 杉生 和久, 武田 健, 国定 俊之, 根津 悠, 川井 章, 尾崎 敏文

    日本整形外科学会雑誌   89 ( 8 )   S1478 - S1478   2015年9月

     詳細を見る

    記述言語:日本語   出版者・発行元:(公社)日本整形外科学会  

    researchmap

  • 腫瘍融解アデノウイルスの骨・軟部腫瘍臨床組織検体に対する感染効率および適応症例の検討

    吉田 晶, 藤原 智洋, 魚谷 弘二, 長谷井 嬢, 山川 泰明, 大森 敏規, 杉生 和久, 武田 健, 国定 俊之, 藤原 俊義, 尾崎 敏文

    日本整形外科学会雑誌   89 ( 8 )   S1673 - S1673   2015年9月

     詳細を見る

    記述言語:日本語   出版者・発行元:(公社)日本整形外科学会  

    researchmap

  • 前外側大腿皮弁で再建した踵骨骨肉腫の1例

    小松原 将, 武田 健, 森田 卓也, 杉生 和久, 魚谷 弘二, 藤原 智洋, 国定 俊之, 尾崎 敏文

    日本整形外科学会雑誌   89 ( 6 )   S1372 - S1372   2015年6月

     詳細を見る

    記述言語:日本語   出版者・発行元:(公社)日本整形外科学会  

    researchmap

  • 四肢長管骨骨欠損に対し処理骨を用いた生物学的再建

    国定 俊之, 武田 健, 藤原 智洋, 魚谷 弘二, 杉原 進介, 尾崎 敏文

    日本整形外科学会雑誌   89 ( 3 )   S684 - S684   2015年3月

     詳細を見る

    記述言語:日本語   出版者・発行元:(公社)日本整形外科学会  

    researchmap

  • 【整形外科領域における集学的診療体制の構築】整形外科腫瘍領域(肉腫)における集学的治療

    国定 俊之, 武田 健, 藤原 智洋, 魚谷 弘二, 杉生 和久, 尾崎 敏文

    整形・災害外科   58 ( 3 )   303 - 313   2015年3月

     詳細を見る

    記述言語:日本語   出版者・発行元:金原出版(株)  

    researchmap

  • 水道水による手術時手洗いは骨軟部腫瘍切除後感染に影響するか

    武田 健, 国定 俊之, 魚谷 弘二, 杉生 和久, 藤原 智洋, 尾崎 敏文

    中部日本整形外科災害外科学会雑誌   58 ( 春季学会 )   110 - 110   2015年3月

     詳細を見る

    記述言語:日本語   出版者・発行元:(一社)中部日本整形外科災害外科学会  

    researchmap

▼全件表示

共同研究・競争的資金等の研究

  • 脊椎脊髄腫瘍に対する腫瘍融解ウイルス療法の確立

    研究課題/領域番号:23K08589  2023年04月 - 2026年03月

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    魚谷 弘二, 吉田 晶, 尾崎 敏文, 藤原 智洋, 田澤 大, 三澤 治夫, 小田 孔明

      詳細を見る

    配分額:4810000円 ( 直接経費:3700000円 、 間接経費:1110000円 )

    researchmap

  • 肉腫におけるエクソソームによる浸潤・転移機序の解明と新規治療法の開発

    研究課題/領域番号:17K10969  2017年04月 - 2020年03月

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    森田 卓也, 魚谷 弘二, 尾崎 敏文, 國定 俊之, 藤原 智洋

      詳細を見る

    配分額:4680000円 ( 直接経費:3600000円 、 間接経費:1080000円 )

    肉腫における特異的な分泌型microRNA/exosomeを細胞株、臨床検体から候補を抽出し、さらにvalidation studyを行うことで各肉腫における特異性の高い遺伝子を特定することが可能であった。次に、それら遺伝しの作用機序を確認するためin vivoでも実験を行い肉腫に特徴的な転移もしくは浸潤に関与しているかを検証したところ、この特異的な遺伝子は転移や浸潤を促進する作用をもっていることを確認した。臨床検体との相互性も確認できたため、特異的なバイオマーカー、また治療薬のない肉腫において、今後の検査や治療に繋がる研究であると考えられる。

    researchmap

  • 体液診断技術を応用した肉腫における新規バイオマーカーの開発と実用化への展開

    研究課題/領域番号:16H05449  2016年04月 - 2019年03月

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    尾崎 敏文, 杉原 進介, 清野 正普, 國定 俊之, 横尾 賢, 森田 卓也, 藤原 智洋, 魚谷 弘二, 落谷 孝広, 吉岡 裕亮, 植田 幸嗣, 上原 健敬

      詳細を見る

    配分額:17550000円 ( 直接経費:13500000円 、 間接経費:4050000円 )

    血中の循環microRNAならびにexosome蛋白に着目し、骨軟部肉腫患者のモニタリングや治療奏功性を判断可能なバイオマーカーの開発を目指した。Ewing肉腫では、proteome解析からexosome表面蛋白を抽出し、細胞培養上清及び患者血清由来exosomeにおける発現を特定した。骨肉腫では、患者血清と細胞株を用いたmicroRNAの網羅的解析から、担癌状態および治療経過を反映する血清miR-25-3pを特定した。滑膜肉腫では、骨肉腫における手法を用いて血清miR-92-3pをリキッドバイオプシーの標的分子として特定し、その発現は治療経過を反映し、他の肉腫組織型との層別化を可能とした。

    researchmap

  • 骨軟部腫瘍に対する腫瘍融解ウイルス療法の個別化医療の確立

    研究課題/領域番号:15K10444  2015年04月 - 2018年03月

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    魚谷 弘二, 尾崎 敏文, 藤原 俊義, 藤原 智洋

      詳細を見る

    配分額:4680000円 ( 直接経費:3600000円 、 間接経費:1080000円 )

    本学で開発された腫瘍融解アデノウイルス(テロメライシン)は効果発現にCARやhTERTの存在が必要で、骨軟部腫瘍へのウイルスの臨床応用にむけた適応症例の選別のために簡便にその評価を行う必要がある。今回、本学で開発したテロメライシンにGFP遺伝子を 組み込み細胞内のウイルス増殖により緑色蛍光を発するテロメスキャンを骨軟部悪性・中間悪性腫瘍に感染させ蛍光発現を評価した。ウイルス投与24時間後の蛍光陽性数はCARの発現強度との相関が有意に認められ、経時的な蛍光の増強にhTERTの発現が関連する傾向が見られた。テロメライシンの肉腫への適応をウイルスの組織片への投与のみで評価できる可能性が示された。

    researchmap