Faculty Profiles - ONO Tsutomu

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マイクロ流体デバイスと液・液相分離を利用した単分散ゲルカプセル調製

安川政宏, 小野努, 木村幸敬

高分子学会予稿集(CD-ROM) 59 ( 2 Disk1 ) 2010

マイクロ流路を利用した単分散複合エマルション調製における制御因子の検討

伊東一行, 安川政宏, 小野努, 木村幸敬

化学工学会年会研究発表講演要旨集(CD-ROM) 75th 2010

1P4-13 An emulsion generating device by an ultrasonic vibration and a microchannel(Poster Session)

Harada Takuya, Kanda Takefumi, Suzumori Koichi, Ono Tsutomu, Iwabuchi Sotaro, Ito Kazuyuki, Ogawara Ken-ichi, Higaki Kazutaka

Proceedings of Symposium on Ultrasonic Electronics 30 111 - 112 2009.11

光線力学的治療に有効な光増感剤内封ナノ粒子の開発

小野努, 廣田健, 木村幸敬, 白石太朗, 大河原賢一, 檜垣和孝, 阪田功

日本レーザー医学会誌 = The Journal of Japan Society for Laser Medicine 30 ( 2 ) 208 - 208 2009.7

高度に制御された巨大孔を有するマイクロカプセルの調製と応用

Eiji Kamio, Tsutomu Ono

ケミカルエンジニヤリング 54, 518-524 2009.6

水性二相系を利用したW/W/Oエマルションの調製とゲルカプセルへの応用

安川政宏, 小野努, 木村幸敬

ポリマー材料フォーラム講演予稿集 18th 2009

2Lp11 Development of high-throughput screening by culture in monodisperse gel particles

WASHIO Naoki, ONO Tsutomu, KIMURA Yukitaka

21 175 - 175 2009

水性二相系を利用したW/W/Oエマルションの調製

安川政宏, 神尾英治, 小野努

化学工学会研究発表講演要旨集 2008 ( 0 ) 643 - 643 2008

Characteristics of compacted plasmid DNA by high pressurization

Tsuyoshi Kimura, Kana Horiuchi, Kimio Kurita, Tsutomu Ono, Hidekazu Yoshizawa, Toshiya Fujisato, Akio Kishida

Nucleic Acids Symposium Series ( 51 ) 343 - 344 2007

In order to investigate the effect of pressure on the tertiary structure of plasmid DNA having the supercoiled and relaxed forms, the solution of plasmid DNA was hydrostatically pressurized at different atmosphere and 40 degrees C for various times. For dynamic light scattering (DLS) measurement of the pressurized plasmid DNA, the hydrodynamic diameters of the super-coiled and relaxed plasmid DNA were decreased with increasing pressure. Also, at constant pressure, a long period of pressure treatment effectively induced the decrease in plasmid DNA. These results suggest that the plasmid DNA was condensed by high hydrostatic pressurization. The circular dichroism (CD) spectrum of the pressurized plasmid DNA was slightly changed. For digestion by S1 nuclease, which selectively cleaves single strand DNA, the pressurized plasmid DNA was easily degraded compared to the non-pressurized plasmid DNA, suggesting that the double helix of plasmid DNA was partly dissociated to single strand by the pressure-induced compaction of plasmid DNA. These results indicate that high hydrostatic pressurization is one of powerful tools for preparing the compacted plasmid DNA.

DOI： 10.1093/nass/nrm172

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Fabrication of a silica monolith micro-bioreactor entrapping highly activated lipase by a two-step sol-gel method

K. Kawakami, D. Abe, T. Urakawa, S. Sakai, T. Ono, H. Ijima

CHISA 2006 - 17th International Congress of Chemical and Process Engineering 2006.12

Micro-bioreactors are effective for high throughput production of expensive products from small amounts of substrates. Lipases are versatile enzymes for chiral syntheses, and highly activated when immobilized in alkyl-substituted silicates by the sol-gel method. For application of the immobilized lipases to a continuous-flow system, a micro-bioreactor loaded with a macroporous silica monolith is well suited, because it can easily be integrated with a separator for optical resolution. We attempted to develop a micro-capillary bioreactor containing a silica monolith-immobilized lipase. A silica monolith without shrinkage during lyophilization of the hydrogel was first formed from a 1:4 mixture of tetramethoxysilane (TMOS) and methyltrimethoxysilane (MTMS). It was then coated with silica precipitates entrapping lipase, derived from a 1:4 mixture of TMOS and n-butyltrimethoxysilane (BTMS). As a result, the monolith treated with the BTMS-based silica entrapping lipase, exhibited approximately ten times higher esterification activity than the non-treated monolith-immobilized lipase derived from the MTMS-based silica.

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マイクロリアクターによるナノエマルションの調製 (特集マイクロ化学技術の実用化)

久保田潤, 小野努, 吉澤秀和

ケミカルエンジニヤリング 51 ( 11 ) 817 - 822 2006.11

Peroxidative catalytic behavior of cytochrome c solubilized in reverse micelles

Tsutomu Ono, Masahiro Goto

Biochemical Engineering Journal 28 ( 2 ) 156 - 160 2006.2

Language：English

Solubilization of horse heart cytochrome c into reverse micelles, a self-assembled nanostructure composed of sodium di-2-ethylhexyl sulfosuccinate (AOT), enhances the peroxidase activity in the presence of hydrogen peroxide. The catalytic activity of cytochrome c hosted in reverse micellar solution is 10-fold higher than that in water, and which depends on Wo, a molar ratio of water to AOT, and pH in aqueous droplets. In addition, the fluorescence intensity based on the tryptophan residue and the visible absorption identifying the axial Met 80-Fe bond imply that the reverse micellar solubilization induces the cleavage of the heme crevice and thus enables the formation of peroxidase-like peroxide-heme complexes. These results suggest that cytochrome c solubilized in reverse micelles provides a homogeneous catalyst as peroxidase available in organic media. © 2005 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.bej.2005.10.005

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2L09-2 Protective effect of oligoglucosamine on the enzymatic degradation of liposome-entrapped DNA

MURAKAMI Sunao, ONO Tsutomu, SAKAI Shinji, IJIMA Hiroyuki, KAWAKAMI Koei

18 220 - 220 2006

Production of nanovesicles entrapping nucleic acids

T Ono, M Morita, S Sakai, H Ijima, K Kawakami

JOURNAL OF BIOTECHNOLOGY 118 S72 - S72 2005.8

2E10-5 Development of DNA-entrapping nano-vesicles using reverse micelles

MURAKAMI Sunao, KAWASHIMA Ayako, ONO Tsutomu, SAKAI Shinji, IJIMA Hiroyuki, KAWAKAMI Koei

17 152 - 152 2005

1H17-2 A novel approach for constructing microvascular system with desired structure

TAKEI Takayuki, SAKAI Shinji, ONO Tsutomu, IJIMA Hiroyuki, KAWAKAMI Koei

17 217 - 217 2005

Mammalian cell encapsulation in monodisperse subsieve-sized (<100 μm) capsules

S. Sakai, K. Kawabata, T. Ono, H. Ijima, K. Kawakami

Transactions - 7th World Biomaterials Congress 1510 2004.12

A method for preparing monodisperse mammalian cell-enclosing subsieve-sized microcapsules from viscous polymer solution was developed. The droplet breakup technique with an immiscible liquid coflowing stream was applied for the preparation of subsieve-sized mammalian cell-enclosing capsules. The reaction was found to enhance cell viability due to reduced resistance to the transport of oxygen, nutrients and metabolites. Mechanical strength and biocompatibility were also found to be enhanced.

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DNA extraction by cationic reverse micelles

Masahiro Goto, Ayumi Momota, Tsutomu Ono

Journal of Chemical Engineering of Japan 37 ( 5 ) 662 - 668 2004.5

Language：English

Deoxyribonucleotide (DNA) was successfully extracted in a few hours by reverse micelles, which were formed and functionalized by a cationic quaternary ammonium surfactant in isooctane. Several parameters which affect the DNA transfer from an aqueous phase to a reverse micellar phase, such as the natures of a surfactant and a solvent, salt concentration in a feed solution, and hydrophobicity of micelles, were investigated. The use of a cationic surfactant was found to yield a high transfer rate of DNA into reverse micelles. We have attributed the DNA transfer to the electrostatic interaction between the cationic surfactant and the negatively charged DNA. We found that the higher the hydrophobicity of a surfactant used to form micelles, the better the DNA transfer efficiency in the forward extraction. Preferential extraction of DNA was achieved by using twin long-alkyl chains of cationic surfactants. However, pH in the aqueous feed solution did not affect the extraction performance of reverse micelles. On the other hand, alcohol, as a co-solvent plays an important role in the back extraction of DNAs. Complete back-extraction of DNAs was achieved without any conformational change by adding an appropriate alcohol to the reverse micellar phase. © 2004 The Society of Chemical Engineers, Japan.

DOI： 10.1252/jcej.37.662

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2F15-3 Effect of cell-suspension viscosity on subsieve-sized capsule diameter

SAKAI Shinji, KAWABATA Kenji, ONO Tsutomu, IJIMA Hiroyuki, KAWAKAMI Koei

16 187 - 187 2004

3I09-4 Preparation of chitosan oligosaccharide-liposome complexes utilizing freeze-thawing method

MURAKAMI Sunao, SAKAI Shinji, ONO Tsutomu, IJIMA Hiroyuki, KAWAKAMI Koei

16 228 - 228 2004

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