Faculty Profiles - HATTORI Takako

Papers - HATTORI Takako

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Cyclic mechanical stress induces extracellular matrix degradation in cultured chondrocytes via gene expression of matrix metalloproteinases and interleukin-1 Reviewed

T Fujisawa, T Hattori, K Takahashi, T Kuboki, A Yamashita, M Takigawa

JOURNAL OF BIOCHEMISTRY 125 ( 5 ) 966 - 975 1999.5

Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE BIOCHEMICAL SOC

To clarify the mechanism of cartilage degradation induced by mechanical stress, we investigated the influence of cyclic tension force (CTF) on the metabolism of cultured chondrocytes. The chondrocytes were exposed to CTF using a Flexercell strain unit. Five or 15 kPa of high frequency CTF significantly inhibited the syntheses of DNA, proteoglycan, collagen, and protein, Fifteen kPa of high frequency CTF induced the expression of interleukin-1 (IL-1), matrix metalloproteinase (MMP)-2 and -9 mRNA, and increased the production of pro- and active-MMP-9. The degradation of proteoglycan was inhibited by and MMP inhibitor, indicating that MMPs are involved in the degradation of proteoglycans induced by high frequency CTF, Moreover, reducing the frequency of CTF from high to low decreased the inhibition of proteoglycan synthesis, These findings suggest that the CTF frequency is one of the key determinants of chondrocyte metabolism. Low magnitude CTF, whether high or low frequency, did not cause the gene expression of cartilage degradation factors, suggesting that this CTF magnitude causes only minor changes in the cartilage matrix, High magnitude and frequency CTF caused the gene expression of IL-1 and MMP-9, followed by increases in the production of MMP-2 and -9 proteins, suggesting that excessive and continuous cyclic mechanical stress induces the production of IL-1 and MMP-9, resulting in cartilage degradation.

DOI： 10.1093/oxfordjournals.jbchem.a022376

Involvement of cis-acting repressive element(s) in the 3'-untranslated region of human connective tissue growth factor gene. Reviewed

Kubota, S, Hattori, T, Nakanishi, T, Takigawa, M

FEBS Letters 450 84 - 88 1999

Demonstration of receptors specific for connective tissue growth factor on a human chondrocytic cell line (HCS-2/8) Reviewed

T Nishida, T Nakanishi, T Shimo, M Asano, T Hattori, T Tamatani, K Tezuka, M Takigawa

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 247 ( 3 ) 905 - 909 1998.6

Language：English Publishing type：Research paper (scientific journal) Publisher：ACADEMIC PRESS INC

The presence of receptors specific for connective tissue growth factor (CTGF) was demonstrated on a human chondrosarcoma-derived chondrocytic cell Line, HCS-2/8. The binding of I-125-labeIed recombinant CTGF to HCS-2/8 cells was inhibited by unlabeled CTGF but not by PDGF-BB or bFGF. Scatchard analysis revealed the presence of two classes of binding sites with Rd values of 18.6 and 259 nM on cells. A cross-linking study revealed the formation of I-12S-CTGF-receptor complex with an apparent molecular weight of 280 kDa, The I-125-CTGF-receptor complex disappeared almost completely on the addition of unlabeled CTGF but not PDGF-BB or bFGF, In addition, the I-125-CTGF-receptor complex was immunoprecipitated with anti-CTGF antiserum but not with anti-PDGF receptor antiserum. These findings suggest that CTGF directly binds to specific receptor molecules on HCS-2/8 cells. (C) 1998 Academic Press.

DOI： 10.1006/bbrc.1998.8895

Nitric oxide mediates interleukin-1-induced gene expression of matrix metalloproteinases and basic fibroblast growth factor in cultured rabbit articular chondrocytes Reviewed

K Sasaki, T Hattori, T Fujisawa, K Takahashi, H Inoue, M Takigawa

JOURNAL OF BIOCHEMISTRY 123 ( 3 ) 431 - 439 1998.3

Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE BIOCHEMICAL SOC

We recently reported that nitric oxide (NO), which is produced by chondrocytes treated with interleukin-1 beta (IL-1), releases basic fibroblast growth factor (bFGF) stored in the matrix of articular chondrocytes, To clarify the mechanism of the IL-l-induced bFGF release, we investigated the production and gene expression of bFGF, matrix metalloproteinases (MMPs), syndecan 3, and inducible NO synthase (iNOS) by IL-l-treated rabbit articular chondrocytes. IL-1 stimulated not only the release of bFGF but also the production of it, Gelatin and casein zymography revealed that IL-1 stimulated the production of not only MMP-9 but also MMP-3, The increase in the production of these MMPs preceded the IL-1-stimulated bFGF release, An MMP inhibitor partially suppressed the release of bFGF, indicating that matrix degradation is at least partially involved in the IL-l-stimulated bFGF release even if increased production of bFGF is related to the release, IL-1 sequentially stimulated mRNA expression of iNOS, membrane type 1-MMP, MMP-9 and -3, and bFGF, in that order, N-G-Monomethyl-L-arginine, an inhibitor of NO production, inhibited gene expression of MMP-9 and bFGF. These findings suggest that elevation of the NO level via iNOS mRNA expression stimulated by IL-1 mediates gene expression and production of MMPs and bFGF, resulting in the release of bFGF, and also reveal molecular mechanisms implicating the degradation of articular cartilage followed by angiogenesis in the synovium in arthritic joints.

DOI： 10.1093/oxfordjournals.jbchem.a021955

Demonstration of receptors for epidermal growth factor on cultured rabbit chondrocytes and regulation of their expression by various growth and differentiation factors. Reviewed

Nishida,T, Nakanishi,T, Shimo,T, Asano,M, Hattori,T, Tamatani,T, Tezuka,K, Takigawa,M

Biochem.Biophys.Res.Commun. 183 14 - 20 1998

Isolation and characterization of a rheumatoid arthritis-specific antigen(RA-A47) from a human chondrocytic cell line(HCS-2/8) Reviewed

Takako Hattori, Takuo Fujisawa, Kazuhiro Sasaki, Yasutaka Yutani, Tohru Nakanishi, Kojiro Takahashi, Masaharu Takigawa

Biochem. Biophys. Res. Commun. 245 ( 3 ) 679 - 683 1998

Authorship：Lead author Publishing type：Research paper (scientific journal)

Two types of 47 kDa antigen specifically recognized by sera from rheumatoid arthritis (RA) patients were isolated from the membrane fraction of a human chondrosarcoma-derived chondrocytic cell line (HCS-2/8) by a 2-step procedure: preparative SDS-PAGE and reverse-phase HPLC. An N-terminal amino acid sequence in one of the 47 kDa antigens, named RA-A47, had 81% homology to that deduced from the DNA sequence of the colligin gene which is reported as human hsp47 gene, and 100% homology to that deduced from the DNA sequence of colligin-2 gene, a homologue of colligin. The RA-A47 cross-reacted with a monoclonal antibody raised against chick heat shock protein (Hsp) 47 and bound to gelatin. The expression of the ra-a47 gene was enhanced by heat shock treatment and TGF-β stimulation. These findings suggest that RA-A47 is a Hsp47-like protein, presumably the product of the colligin-2 gene, and that a collagen-specific molecular chaperone(s) such as Hsp47 and/or RA-A47 is involved in cartilage destruction in RA.

DOI： 10.1006/bbrc.1998.8505

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Specific serum antibodies against membranous proteins of a human immortal chondrocytic cell line (HCS-2/8) in rheumatoid arthritis and their relationship to the natural history of this disease Reviewed

Akira Sakawa, Yasutaka Yutani, Kentaro Inui, Akira Shimazu, Yoshiki Yamano, Akira Kinosita, Fujio Suzuki, Takako Hattori, Masaharu Takigawa

Journal of Bone and Mineral Metabolism 14 ( 3 ) 146 - 152 1996.9

Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC

An immortal human chondrocytic cell line (HCS-2/8) derived from a chondrosarcoma was used as a source of human antigens to find humoral antibodies to cell surface proteins of human chondrocytes in sera from patients with rheumatoid arthritis (RA). Membrane fractions prepared from the cell line were subjected to Western blot analysis using RA and normal sera as probes. RA sera recognized about a dozen bands, but three of these bands, with molecular weights of 105 kDa, 68 kDa, and 47 kDa, were found to be specific for the RA sera (P < 0.05). These bands disappeared following V8 protease digestion, indicating that they were proteins. Among patients with 4 years or more of RA disease activity, reactivity against 105-kDa and 68-kDa proteins was relatively high in those whose joints showed a high degree of erosion. We suspect that levels of these two antibodies are suggestive of changes associated with the natural course of RA.

DOI： 10.1007/bf02239482

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Other Link： http://link.springer.com/article/10.1007/BF02239482/fulltext.html

Novel FNR homologues identified in four representative oral facultative anaerobes: Capnocytophaga ochracea, Capnocytophaga sputigena, Haemophilus aphrophilus, and Actinobacillus actinomycetemcomitans Reviewed

T Hattori, K Takahashi, T Nakanishi, H Ohta, K Fukui, S Taniguchi, M Takigawa

FEMS MICROBIOLOGY LETTERS 137 ( 2-3 ) 213 - 220 1996.4

Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCIENCE BV

Based upon DNA sequence data and positive immunochemical reactivity of expressed protein, novel homologues of the FNR family were identified in four representative oral facultative anaerobes: Capnocytophaga ochracea, Capnocytophaga sputigena, Haemophilus aphrophilus, and Actinobacillus actinomycetemcomitans. The similarity to E. coli FNR and to HlyX (itself 71% similar to E. coli FNR, while regulating expression of hemolysin operon in Actinobacillus pleuropneumoniae) was estimated from the deduced partial amino acid sequence to be, in the above order of tested species, 98, 98, 86, and 85%, and 75, 75, 88, and 88%, respectively. The phylogenetic relatedness indicates a rather closer link of HlyX to the FNR homologues from both pathogens, H. aphrophilus and A. actinomycetemcomitans. The possibility that the A. actinomycetemcomitans FNR homologue functions as a redox-sensing transcriptional factor to regulate, in addition to anaerobic respiration, microaerobic expression of the leukotoxin operon (Itx gene) is suggested.

DOI： 10.1111/j.1574-6968.1996.tb08108.x

Nitric oxide mediates interleukin-1-induced matrix degradation and basic fibroblast groth factor release in cultured rabbit articular chondrocytes: A possible mechanism of pathological neovascularization in arthritis. Reviewed

Tamura, T, Nakanishi, T, Kimura, Y, Hattori, T, Sasaki, K, Norimatsu, H, Takahashi, K, Takigawa, M

Endocrinology 137 ( 9 ) 3729 - 3737 1996

Nitric oxide mediates interleukin-1-induced matrix degradation and basic fibroblast growth factor release in cultured rabbit articular chondrocytes: A possible mechanism of pathological neovascularization in arthritis

Tomoo Tamura, Tohru Nakanishi, Yusuke Kimura, Takako Hattori, Kazuhiro Sasaki, Hiromichi Norimatsu, Kojiro Takahashi, Masaharu Takigawa

Endocrinology 137 ( 9 ) 3729 - 3737 1996

Publishing type：Research paper (scientific journal)

Prolonged incubation with interleukin-1β (IL-1) induced the release of large amounts of NO and subsequently inhibited DNA synthesis and the biosynthesis and accumulation of proteoglycans in cultured rabbit articular chondrocytes (RAC). IL-1 also inhibited DNA synthesis in bovine aortic endothelial cells (BAE). On the other hand, DNA synthesis in BAE cocultured with RAC was not inhibited by prolonged incubation with IL-1. Moreover, conditioned media from RAC incubated for a long period with IL-1 stimulated DNA synthesis in BAE alone. This growth stimulatory activity was mainly due to the release of basic fibroblast growth factor, a heparin-binding growth factor, into RAC culture. Gelatin zymography of the RAC culture medium revealed that IL-1 increased the production of matrix met alloproteinase-2 (MMP-2) and MMP-9. N(G)-Monomethyl-L-arginine, an inhibitor of NO synthesis, inhibited all of these actions of IL-1. These results indicate that NO from RAC treated with IL-1 stimulates MMPs, which, in turn, degrade the extracellular matrix produced by RAC, resulting in the release of large amounts of basic fibroblast growth factor stored in the matrix, which then stimulates adjacent BAE proliferation. Thus, NO produced from RAC treated with IL-1 may modulate angiogenesis in the synovium of arthritic patients.

DOI： 10.1210/endo.137.9.8756539

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Expression of nerve growth factor family neurotrophins in a mouse osteoblastic cell line

Tohru Nakanishi, Kojiro Takahashi, Chiharu Aoki, Kiyoshi Nishikawa, Takako Hattori, Shigehiko Taniguchi

Biochemical and Biophysical Research Communications 198 ( 3 ) 891 - 897 1994.2

Publishing type：Research paper (scientific journal)

With cultured osteoblastic cells, clone MC3T3-E1 derived from newborn mouse calvaria, the mRNAs encoding three representative neurotrophins, namely, nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3), were proved in estimation by the polymerase chain reaction (PCR) method. The increase and then subsequent decrease of all three mRNAs within the proliferation phase were followed by their gradual increase in the differentiation phase, with a tendency of enhancement by exogenous TGF-β as was particularly evident in the case of NGF. These findings were further substantiated by identification of NGF-like neurotrophins in the conditioned medium of the osteoblastic cells by an immunoblotting and neurite extension assay. © 1994 Academic Press, Inc.

DOI： 10.1006/bbrc.1994.1127

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REPRESSION OF IN-VITRO TRANSCRIPTION OF THE ESCHERICHIA-COLI FNR AND NARX GENES BY FNR PROTEIN Reviewed

K TAKAHASHI, T HATTORI, T NAKANISHI, T NOHNO, N FUJITA, A ISHIHAMA, S TANIGUCHI

FEBS LETTERS 340 ( 1-2 ) 59 - 64 1994.2

Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCIENCE BV

In facultative anaerobes, the anaerobic expression of respiratory genes is regulated by a transcriptional activator, FNR. Transcription in vitro of the E. coli fnr gene was repressed by its product, FNR. The transcription of the E. coli nar X gene encoding the nitrate sensor protein was likewise repressed. DNA truncation experiments for fnr and nar X genes indicated that multiple anaero-boxes in each promoter region are essential for repression by the FNR protein, but they also suggest that factor-independent upstream activation signals are operating with these promoters.

DOI： 10.1016/0014-5793(94)80173-8

Expression of nerve growth factor family neurotrophins in a mouse osteoblastic cell line. Reviewed

Nakanishi, T, Takahashi, K, Aoki, C, Nishikawa, K, Hattori, T, Taniguchi, S

Biochem. Biophys. Res. Commun. 198 ( 3 ) 891 - 897 1994

Differential expression of homeobox-containing genes Msx-1 and Msx-2 and homeoprotein Msx-2 expression during chick craniofacial development. Reviewed

Nishikawa, K, Nakanishi, T, Aoki, C, Hattori, T, Takahashi, K, Taniguchi, S

Biochem. Mol. Biol. Inter. 32 ( 4 ) 763 - 771 1994

Studies on phosphorylated transcriptional regulator(NarL)for DBE. coli nar(/)/DB operon by ³¹P-NMR spectroscopy.

K. Takahashi, T. Hattori, H. Shindo, S. Noji, T. Nohno, S. Taniguchi

Biochemistry and Molecular Biology International 31 ( 1 ) 161 - 168 1993

Publishing type：Research paper (scientific journal)

The sequential transphosphorylation from autophosphorylated nitrate-sensing protein (NarX) to the transcriptional regulator protein (NarL), both operating in signal transduction to control the expression of the respiratory nitrate reductase (nar) operon in E. coli, was demonstrated with an in vitro reconstructed system to function similarly to other bacterial two-component regulatory systems. Over-expression system established by means of the pT7 promoter/polymerase provided both NarX and NarL proteins to reconstruct the in vitro transphosphorylation system. The phosphorylated NarL was detected, and the unstable phosphorylated group was directly assigned to acyl phosphate in the in vitro system by 31P-NMR spectroscopy.

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FNR-like protein as a regulator for the gene expression of anaerobic respiratory system in oral bacteria. Reviewed

Takahashi Kojiro, Hattori Takako, Ohta Hiroyuki, Noji Sumihare, Kato Keijiro, Taniguchi Shigehiko

Japanese Journal of Oral Biology 34 ( 5 ) 459 - 466 1992

Language：Japanese Publisher：Japanese Association for Oral Biology

TheE. coliFNR protein is known as a molecular oxygene sensor and a specific activator for the gene expression of anaerobic respiratory system. In order to survey metabolic response toward molecular oxygen in oral bacteria from viewpoint of FNR-like protein participation, the expression of FNR-like protein in five representative strains of oral bacteria (Actinobacillus actinomycetemcomitansand Streptococcus sanguis as facultative anaerobe, Wolinella recta as microaerophile, and Prevotella intermedia and Fusobacterium nucleatumas strict anaerobe) was investigated with the Western blot method using an anti-FNR rabbit serum prepared against the purifiedE. coliFNR. For only A. actinomycetemcomitansas a facultative anaerobe, the Western blot test was positive, whereas negative for other four strains. This implies the possibility that in A. actinomycetemcomitans, a regulatory mechanism similar to that in E. coli functions for the expression of anaerobic respiratory genes.<BR>The expression level of FNR-like protein in A. actinomycetemcomitans was found to be enhanced with decreasing rate of the fructose-limited growth at the redox potential fixed around-400 mV, andbe maximum at the redox potential around-250 mV with the growth rate fixed at 0.11 h-1 under the anaerobic conditions.

DOI： 10.2330/joralbiosci1965.34.459

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