Updated on 2025/06/14

写真a

 
TSAI, Pi-Cheng
 
Organization
Scheduled update Special-Appointment Assistant Professor
Position
Special-Appointment Assistant Professor
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Degree

  • PhD ( 2020.3   The University of Tokyo )

Education

  • The University of Tokyo   大学院農学生命科学研究科   応用生命工学専攻

    2016.4 - 2020.3

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    Country: Japan

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  • Chang Gung University, Taiwan   Graduate Institute of Biomedical Sciences   Department of Biochemistry and Molecular Biology

    2013.9 - 2015.7

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    Country: Taiwan, Province of China

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Research History

  • Okayama University   Research Institute for Interdisciplinary Science,Advanced Research Field   Assistant Professor

    2025.4

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    Country:Japan

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  • Okayama University   The Research Institute for Interdisciplinary Science   Assistant Professor

    2022.9 - 2025.3

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    Country:Japan

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Professional Memberships

  • Japanese Society of Photosynthesis Research

    2025.1

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Papers

  • Structure of a photosystem II-FCPII supercomplex from a haptophyte reveals a distinct antenna organization Reviewed

    Romain La Rocca, Koji Kato, Pi-Cheng Tsai, Yoshiki Nakajima, Fusamichi Akita, Jian-Ren Shen

    Nature Communications   16   2025.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41467-025-59512-9

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  • Structural study of the chlorophyll between Lhca8 and PsaJ in an Antarctica green algal photosystem I-LHCI supercomplex revealed by its atomic structure Reviewed International journal

    Pi-Cheng Tsai, Koji Kato, Jian-Ren Shen, Fusamichi Akita

    Biochimica et Biophysica Acta (BBA) - Bioenergetics   1866 ( 2 )   149543 - 149543   2025.2

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    Coccomyxa subellipsoidea is an oleaginous, non-motile unicellular green microalga isolated from Antarctica, and is an attractive candidate for CO2 fixation and biomass production. C. subellipsoidea is the first polar green alga whose genome has been sequenced. Understanding the structure of photosystems from C. subellipsoidea can provide more information about the conversion of light energy into chemical energy under extreme environments. Photosystems I (PSI) is one of the two photosystems highly conserved from cyanobacteria to vascular plants, and associates with a large amount of outer light-harvesting complex (LHC) which absorb light energy and transfer them to the core complex. Here, we determined the structure of the PSI-10 LHCIs and PSI-8 LHCIs supercomplexes from C. subellipsoidea at 1.92 Å and 2.06 Å resolutions by cryo-electron microscopy, respectively. The supercomplex is similar to PSI-LHCI from other green algae, whereas a large amount of water molecules is observed in our structure because of the high-resolution map. Two novel chlorophylls (Chls), Chl a321 in Lhca4 and Chl a314 in Lhca8, are observed at the lumenal side in our structure, in which Lhca8-Chl a314 provides a potential excitation energy transfer (EET) pathway between the inner-belt of LHCI and the core at the lumenal side. A total of three major EET pathways from LHCIs to PSI core are proposed, and C. subellipsoidea might adapt to the extreme environment by transferring energy in these three different EET pathways instead of by two major pathways proposed in other organisms.

    DOI: 10.1016/j.bbabio.2025.149543

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  • The α- and β-Subunit Boundary at the Stem of the Mushroom-Like α 3 β 3 -Type Oxygenase Component of Rieske Non-Heme Iron Oxygenases Is the Rieske-Type Ferredoxin-Binding Site Reviewed International journal

    Pi-Cheng Tsai, Joydeep Chakraborty, Chiho Suzuki-Minakuchi, Tohru Terada, Tatsurou Kotake, Jun Matsuzawa, Kazunori Okada, Hideaki Nojiri

    Applied and Environmental Microbiology   88 ( 15 )   e0083522   2022.8

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:American Society for Microbiology  

    We clarified the critical amino acid residues of the oxygenase component (Oxy) of Rieske non-heme iron oxygenase (RO) for binding with Rieske-type ferredoxin (Fd). Our results showed that Rieske-type Fd-binding site is commonly located at the stem (side-wise site) of the mushroom-like α 3 β 3 quaternary structure in many ROs.

    DOI: 10.1128/aem.00835-22

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  • Mechanism of electron transfer between ferredoxin and α3β3-type oxygenase components of Rieske non-heme iron oxygenase

    Pi-Cheng Tsai

    2020.3

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    Authorship:Lead author, Last author, Corresponding author   Language:English   Publishing type:Doctoral thesis  

    DOI: 10.15083/0002004915

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MISC

  • Structural basis of the electron transfer mechanism in heterohexamer-type ring-hydroxylating oxygenases

    水口千穂, 水口千穂, TSAI Pi-Cheng, 寺田透, 寺田透, 野尻秀昭, 野尻秀昭

    KEK Progress Report (Web)   ( 2023-2 )   2023

Presentations

  • Energy transfer pathway between Lhca8 and PsaJ in a green algal photosystem I-LHCI supercomplex revealed by its atomic structural analysis

    Pi-Cheng Tsai, Koji Kato, Jian-Ren Shen, Fusamichi Akita

    The 66th Annual Meeting of the Japanese Society of Plant Physiologists  2025.3.14 

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    Event date: 2025.3.14 - 2025.3.16

    Language:English   Presentation type:Oral presentation (general)  

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  • Cyro-EM structures of PSI-LHCI supercomplex from the model organism Marchantia polymorpha

    Pi-Cheng Tsai, Hiroyasu Motose, Jian-Ren Shen, Fusamichi Akita

    The 2025 annual meeting of the Japan Society for Bioscience, Biotechnology and Agrochemistry  2025.3.8 

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    Event date: 2025.3.4 - 2025.3.8

    Language:English   Presentation type:Poster presentation  

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  • Cryo-EM structure of a photosystem I supercomplex from an oleaginous green alga Coccomyxa subellipsoidea at an atomic resolution

    Pi-Cheng Tsai, Jian-Ren Shen, Fusamichi Akita

    2nd Asia-Oceania International Congress on Photosynthesis  2024.9.20 

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    Event date: 2024.9.18 - 2024.9.21

    Language:English   Presentation type:Oral presentation (general)  

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  • Cryo-EM structure of a photosystem I supercomplex from oleaginous green alga Coccomyxa subellipsoidea

    Pi-Cheng Tsai, Fusamichi Akita, Jian-Ren Shen

    IUPAB 2024 (International Union of Pure and Applied Biophysics)  2024.6.26 

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    Event date: 2024.6.24 - 2024.6.28

    Language:English   Presentation type:Poster presentation  

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  • Cryo-EM structure of a photosystem I supercomplex from oleaginous green alga Coccomyxa subellipsoidea

    Pi-Cheng Tsai, Fusamichi Akita, Jian-Ren Shen

    The 2024 annual meeting of the Japan Society for Bioscience, Biotechnology and Agrochemistry  2024.3.26 

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    Event date: 2024.3.24 - 2024.3.27

    Language:English   Presentation type:Oral presentation (general)  

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  • Growing up in the cracks: Exploring how to survive in Japan’s academic field Invited

    Pi-Cheng Tsai

    JTBA 2023 annual meeting - Japan-Taiwan Biotechnology Trend Forum  2023.12.16 

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    Event date: 2023.12.16

    Language:Chinese   Presentation type:Oral presentation (invited, special)  

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  • The roles of phosphorylation in photosystem I supercomplex in state I-state II transition.

    Pi-Cheng Tsai, Fusamichi Akita, Jian-Ren Shen

    Taiwan-Japan Plant Biology 2023  2023.10.14 

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    Event date: 2023.10.13 - 2023.10.15

    Language:English   Presentation type:Poster presentation  

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  • Binding manner between ferredoxin and α3β3-type oxygenase components in Rieske non-heme iron dioxygenase.

    TSAI Pi-Cheng, CHAKRABORTY Joydeep, SUZUKI-MINAKUCHI Chiho, OKADA Kazunori, NOJIRI Hideaki

    The 19th Annual Meeting of the Protein Science Society of Japan  2019.6.25 

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    Event date: 2019.6.24 - 2019.6.26

    Language:English   Presentation type:Poster presentation  

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  • Electron transfer mechanisms among the ferredoxin andα3β3-type oxygenase component of Rieske non-heme iron oxygenase.

    TSAI Pi-Cheng, CHAKRABORTY Joydeep, SUZUKI-MINAKUCHI Chiho, OKADA Kazunori, NOJIRI Hideaki

    The 2019 annual meeting of the Japan Society for Bioscience, Biotechnology and Agrochemistry  2019.3.27 

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    Event date: 2019.3.24 - 2019.3.27

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • Electron transfer from ferredoxin and α3β3-type oxygenase component of Rieske non-heme iron cumene 2,3-dioxygenase from Pseudomonas fluorescens IP01

    TSAI Pi-Cheng, CHAKRABORTY Joydeep, SUZUKI-MINAKUCHI Chiho, OKADA Kazunori, NOJIRI Hideaki

    The 2018 annual meeting of the Japan Society for Bioscience, Biotechnology and Agrochemistry  2018.3.16 

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    Event date: 2018.3.15 - 2018.3.18

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • Determination of energy transfer intra- and inter-proteins by biochemical and structural analysis. Invited

    Pi-Cheng Tsai

    National Taiwan University, Taiwan  2024.9.6 

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    Language:English   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

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