2022/01/04 更新

写真a

ウシジマ コウイチロウ
牛島 幸一郎
USHIJIMA Koichiro
所属
環境生命科学学域 准教授
職名
准教授
外部リンク

学位

  • 博士(理学) ( 2002年3月   横浜市立大学 )

  • 修士(理学) ( 1999年3月   横浜市立大学 )

  • 学士(農学) ( 1997年3月   東京農工大学 )

研究分野

  • 環境・農学 / 園芸科学

 

論文

  • Combined signal sequence trap and macroarray analysis identifies genes associated with differential fruit softening characteristics during ripening in European and Chinese pears 査読

    Mercy W. Mwaniki, Oscar W. Mitalo, Eric G. Mworia, Willis O. Owino, Kyoko Hiwasa-Tanase, Jocelyn K. C. Rose, Koh Aoki, Tomoya Esumi, Takashi Kawai, Ryohei Nakano, Koichiro Ushijima, Yasutaka Kubo

    POSTHARVEST BIOLOGY AND TECHNOLOGY   174   2021年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.postharvbio.2020.111436

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  • Association of T2/S-RNase With Self-Incompatibility of Japanese Citrus Accessions Examined by Transcriptomic, Phylogenetic, and Genetic Approaches. 査読 国際誌

    Chitose Honsho, Koichiro Ushijima, Misa Anraku, Shuji Ishimura, Qibin Yu, Frederick G Gmitter Jr, Takuya Tetsumura

    Frontiers in plant science   12   638321 - 638321   2021年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3389/fpls.2021.638321

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  • Molecular Mechanism Underlying Derepressed Male Production in Hexaploid Persimmon 査読

    Kanae Masuda, Naoko Fujita, Ho-Wen Yang, Koichiro Ushijima, Yasutaka Kubo, Ryutaro Tao, Takashi Akagi

    FRONTIERS IN PLANT SCIENCE   11   2020年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3389/fpls.2020.567249

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  • Low temperature modulates natural peel degreening in lemon fruit independently of endogenous ethylene 査読

    Oscar W Mitalo, Takumi Otsuki, Rui Okada, Saeka Obitsu, Kanae Masuda, Yuko Hojo, Takakazu Matsuura, Izumi C Mori, Daigo Abe, William O Asiche, Takashi Akagi, Yasutaka Kubo, Koichiro Ushijima

    Journal of Experimental Botany   71 ( 16 )   4778 - 4796   2020年8月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1093/jxb/eraa206

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    その他リンク: http://academic.oup.com/jxb/article-pdf/71/16/4778/33573305/eraa206.pdf

  • Genome-wide study on the polysomic genetic factors conferring plasticity of flower sexuality in hexaploid persimmon 査読

    Kanae Masuda, Eiji Yamamoto, Kenta Shirasawa, Noriyuki Onoue, Atsushi Kono, Koichiro Ushijima, Yasutaka Kubo, Ryutaro Tao, Isabelle M. Henry, Takashi Akagi

    DNA RESEARCH   27 ( 3 )   2020年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1093/dnares/dsaa012

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  • モモ‘桃水’のエチレン生成と軟化特性 査読

    河井 崇, 秋田香雅里, 渡邉咲音, 深松陽介, 高田大輔, 佐藤 守, 牛島幸一郎, 福田文夫, 中野龍平

    園芸学研究   19 ( 1 )   61 - 67   2020年1月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

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  • Postharvest Properties of Ultra-Late Maturing Peach Cultivars and Their Attributions to Melting Flesh (M) Locus: Re-evaluation of M Locus in Association With Flesh Texture. 国際誌

    Ryohei Nakano, Takashi Kawai, Yosuke Fukamatsu, Kagari Akita, Sakine Watanabe, Takahiro Asano, Daisuke Takata, Mamoru Sato, Fumio Fukuda, Koichiro Ushijima

    Frontiers in plant science   11   554158 - 554158   2020年

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3389/fpls.2020.554158

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  • ‘Passe Crassane’ pear fruit (Pyrus communis L.) ripening: revisiting the role of low temperature via integrated physiological and transcriptome analysis 査読

    Oscar Witere MITALO, Yasuaki Tosa, Sumire Tokiwa, Yuki Kondo, Azimullah Azimi, Yuko Hojo, Takakazu Matsuura, Izumi Mori, Ryohei NAKANO, Takashi Akagi, Koichiro Ushijima, Yasutaka Kubo

    Postharvest Biology and Technology   158   110949 - 110949   2019年12月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.postharvbio.2019.110949

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  • A Sequence Resource of Autosomes and Additional Chromosomes in the Peach Pathotype of Alternaria alternata 査読

    Koichiro Ushijima, Mikihiro Yamamoto

    MOLECULAR PLANT-MICROBE INTERACTIONS   32 ( 10 )   1273 - 1276   2019年10月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1094/MPMI-04-19-0093-A

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  • Low temperature storage stimulates fruit softening and sugar accumulation without ethylene and aroma volatile production in kiwifruit 査読

    Oscar W. Mitalo, Sumire Tokiwa, Yuki Kondo, Takumi Otsuki, Ivan Galis, Katsuhiko Suezawa, Ikuo Kataoka, Anh T. Doan, Ryohei Nakano, Koichiro Ushijima, Yasutaka Kubo

    Frontiers in Plant Science   10   1 - 15   2019年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3389/fpls.2019.00888

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  • Comparative analysis of fruit ripening and associated genes in two kiwifruit cultivars (‘Sanuki Gold’ and ‘Hayward’) at various storage temperatures 査読

    Oscar Witere Mitalo, William Olubero Asiche, Yuka Kasahara, Yasuaki Tosa, Sumire Tokiwa, Koichiro Ushijima, Ryohei Nakano, Yasutaka Kubo

    Postharvest Biology and Technology   147   20 - 28   2019年1月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.postharvbio.2018.08.017

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  • Aberrant Stamen Development is Associated with Parthenocarpic Fruit Set through Up-regulation of Gibberellin Biosynthesis in Tomato 査読

    Okabe, Yoshihiro, Yamaoka, Tatsuya, Ariizumi, Tohru, Ushijima, Koichiro, Kojima, Mikiko, Takebayashi, Yumiko, Sakakibara, Hitoshi, Kusano, Miyako, Shinozaki, Yoshihito, Pulungan, Sri I, Kubo, Yasutaka, Nakano, Ryohei, Ezura, Hiroshi

    Plant & Cell Physiology   60 ( 1 )   38 - 51   2019年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Parthenocarpy, a process in which fruit set occurs without fertilization, leads to the production of seedless fruit. A number of floral homeotic mutants with abnormal stamen development exhibit parthenocarpic fruit set. Flower development is thought to repress ovary growth before anthesis. However, the mechanism of parthenocarpic fruit development caused by aberrant flower formation is poorly understood. To investigate the molecular mechanism of parthenocarpic fruit development in floral homeotic mutants, we performed functional analysis of Tomato APETALA3 (TAP3) by loss-of-function approaches. Organ-specific promoter was used to induce organ-specific loss of function in stamen and ovary/fruit. We observed increased cell expansion in tap3 mutants and TAP3-RNAi lines during parthenocarpic fruit growth. These were predominantly accompanied by the up-regulation of GA biosynthesis genes, including SlGA20ox1, SlGA20ox2, and SlGA20ox3, as well as reduced expression of the GA-inactivating gene SlGA2ox1 and the auxin signaling gene SlARF7 involved in a crosstalk between GA and auxin. These transcriptional profiles are in agreement with the GA levels in these lines. These results suggest th

    DOI: 10.1093/pcp/pcy184

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  • Comparative Mapping of the ASTRINGENCY Locus Controlling Fruit Astringency in Hexaploid Persimmon (Diospyros kaki Thunb.) with the Diploid D. lotus Reference Genome 査読

    Nishiyama Soichiro, Onoue Noriyuki, Kono Atsushi, Sato Akihiko, Ushijima Koichiro, Yamane Hisayo, Tao Ryutaro, Yonemori Keizo

    HORTICULTURE JOURNAL   87 ( 3 )   315 - 323   2018年7月

  • Comparative transcriptome analysis reveals distinct ethylene-independent regulation of ripening in response to low temperature in kiwifruit 査読

    William O. Asiche, Oscar W. Mitalo, Yuka Kasahara, Yasuaki Tosa, Eric G. Mworia, Willis O. Owino, Koichiro Ushijima, Ryohei Nakano, Kentaro Yano, Yasutaka Kubo

    BMC Plant Biology   18 ( 1 )   2018年3月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1186/s12870-018-1264-y

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  • Characterization of ripening-related genes involved in ethylene-independent low temperature-modulated ripening in ‘rainbow red’ kiwifruit during storage and on-vine 査読

    Oscar Witere Mitalo, William Olubero Asiche, Yuka Kasahara, Yasuaki Tosa, Willis Omondi Owino, Eric Gituma Mworia, Koichiro Ushijima, Ryohei Nakano, Yasutaka Kubo

    Horticulture Journal   87 ( 3 )   421 - 429   2018年

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.2503/hortj.OKD-035

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  • Nondestructive detection of split pit in peaches using an acoustic vibration method 査読

    Ryohei Nakano, Hidemi Akimoto, Fumio Fukuda, Takashi Kawai, Koichiro Ushijima, Yosuke Fukamatsu, Yasutaka Kubo, Yuichiro Fujii, Ken Hirano, Kunihisa Morinaga, Naoki Sakurai

    Horticulture Journal   87 ( 2 )   281 - 287   2018年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2503/hortj.OKD-094

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  • Effect of Storage Temperature on Fruit Ripening in Three Kiwifruit Cultivars 査読

    William Olubero Asiche, Oscar Witere Mitalo, Yuka Kasahara, Yasuaki Tosa, Eric Gituma Mworia, Koichiro Ushijima, Ryohei Nakano, Yasutaka Kubo

    HORTICULTURE JOURNAL   86 ( 3 )   403 - 410   2017年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2503/hortj.OKD-028

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  • Two Members of the Aluminum-Activated Malate Transporter Family, SlALMT4 and SlALMT5, are Expressed during Fruit Development, and the Overexpression of SlALMT5 Alters Organic Acid Contents in Seeds in Tomato (Solanum lycopersicum) 査読

    Takayuki Sasaki, Yoshiyuki Tsuchiya, Michiyo Ariyoshi, Ryohei Nakano, Koichiro Ushijima, Yasutaka Kubo, Izumi C. Mori, Emi Higashiizumi, Ivan Galis, Yoko Yamamoto

    PLANT AND CELL PHYSIOLOGY   57 ( 11 )   2367 - 2379   2016年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1093/pcp/pcw157

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  • Extension of Shelf-life by Limited Duration of Propylene and 1-MCP Treatments in Three Kiwifruit Cultivars 査読

    William Olubero Asiche, Eric Gituma Mworia, Chisato Oda, Oscar Witere Mitalo, Willis Omondi Owino, Koichiro Ushijima, Ryohei Nakano, Yasutaka Kubo

    HORTICULTURE JOURNAL   85 ( 1 )   76 - 85   2016年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.2503/hortj.MI-066

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  • Effect of postharvest short-term radiation of near infrared light on transpiration of lettuce leaf 査読

    Ayako Kozuki, Yutaka Ishida, Kazumasa Kakibuchi, Toshihiro Mishima, Naoki Sakurai, Yoshiyuki Murata, Ryohei Nakano, Koichiro Ushijima, Yasutaka Kubo

    POSTHARVEST BIOLOGY AND TECHNOLOGY   108   78 - 85   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    We investigated the physiological effects of short-term postharvest near infrared (NIR) radiation on relative transpiration rates, stomatal apertures, and reactive oxygen species (ROS) levels in guard cells on excised young lettuce leaves and on transpiration of leaf lettuce at commercial maturity. When the young leaves were radiated by NIR of wavelengths longer than 850 nm at 100 mu mol m(-2) s(-1) for short duration (10-60) min, relative transpiration rates during subsequent storage were reduced, but not by visible light radiation and by longer radiation (180 min) of NIR. The reduction in transpiration rates by the short-term NIR radiation was greater at 10 degrees C than at 25 degrees C under both dark and light conditions during subsequent storage. The short-term NIR radiation enhanced stomatal closure and ROS accumulation in guard cells of young lettuce leaves. These results indicate that the suppression of transpiration by short-term NIR radiation is likely to be mediated through stomatal closure due to NIR-induced ROS accumulation. The reduction of transpiration by short-term NIR radiation was obtained not only in excised young leaves but also in leaf lettuce at commercial maturity, resulting in keeping freshness. The short-term NIR radiation could be an additional means to extend shelf life of leaf vegetables. (C) 2015 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.postharvbio.2015.05.010

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  • Genetic Control of Floral Morph and Petal Pigmentation in Linum grandiflorum Desf., a Heterostylous Flax 査読

    Koichiro Ushijima, Kazuo Ikeda, Ryohei Nakano, Miyo Matsubara, Yuri Tsuda, Yasutaka Kubo

    HORTICULTURE JOURNAL   84 ( 3 )   261 - 268   2015年7月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC HORTICULTURAL SCI  

    Two types of floral morph, called thrum and pin, occur in heterostylous species. The thrum and pin flowers have shorter and longer styles, respectively. Heterostyly in Linum has been studied since Darwin's era. The floral morph, self-incompatibility, and related phenotypes have been well characterized using a natural population, but genetic analysis using a segregated population has not been reported, and the mode of inheritance of heterostyly in Linum remains to be investigated. We prepared a segregated population by crossing thrum and pin flowers of Linum grandiflorum Desf. and investigated style and stamen lengths. On the basis of the style to stamen length ratio, the population could be divided into thrum and pin clearly at a ratio of 1:1. Style length of pins was 1.6 times longer than that of thrums. To investigate the factor regulating the difference in style length, we further measured the style cell length of thrums and pins. The style cell length of pins was longer than that of thrums, whose rate was comparable to the rate of the style length ratio between thrum and pin flowers. These findings indicate that the floral morph of heterostyly in Linum is controlled by a single diallelic locus, and that a difference in the cell expansion rate caused thrum and pin morphs in the style, such as in a typical heterostylous species. PCR genotyping showed that TSS1, an S candidate gene reported previously, cosegregated completely with the thrum phenotype, indicating strong linkage between the S locus and TSS1. Furthermore, three flower colors (red, pink, and white) were observed at a 1:2:1 ratio, and no white thrum flowers or red pin flowers were found in this population. These flower color phenotypes could also be controlled by a diallelic locus, whose two alleles (R and r) would be incompletely dominant, and therefore, may be linked to the S locus.

    DOI: 10.2503/hortj.MI-045

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  • Double flower formation induced by silencing of C-class MADS-box genes and its variation among petunia Cultivars 査読

    Siti Hajar Noor, Koichiro Ushijima, Ayaka Murata, Kaori Yoshida, Miki Tanabe, Tomoki Tanigawa, Yasutaka Kubo, Ryohei Nakano

    SCIENTIA HORTICULTURAE   178   1 - 7   2014年10月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Double flowers induced by virus-induced gene silencing (VIGS) of two C-class MADS-box genes, pMADS3 and FBP6, were investigated in four cultivars of Petunia hybrida. In flowers induced by either pMADS3-VIGS or FBP6-VIGS, only small changes in commercial appearance were recognized regardless of cultivar, whereas in those induced by pMADS3/FBP6-VIGS, complete conversion of stamens into petaloid tissues and marked enlargement of upper limb-like tissues were observed, resulting in a decorative appearance in all the four cultivars. In whorl 4, cultivar-dependent conversion of carpels into new flowers was noted in pMADS3/FBP6-VIGS flowers. Of the four cultivars, only 'Mambo Purple' exhibited the development of new flowers instead of carpels and the emergence of ectopic new flowers from the axil of whorl 3 organs, which created an ornamental appearance with a high commercial value. Investigation of large and small petaloid stamens induced by pMADS3/FBP6-VIGS and pMADS3-VIGS, respectively, revealed only small differences in cell size compared to the large difference in total surface area. Quantitative RT-PCR analysis of SQUAMOSA/AP1/FRU type A-class genes, FBP29, PFG, and FBP26, showed no close relationship between the expression of those genes and petaloid stamen size. The suppressed C-class gene function at the late stage of flower development has little influence on the final size of petaloid tissue. (C) 2014 Elsevier IV. All rights reserved.

    DOI: 10.1016/j.scienta.2014.07.029

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  • Isolation of the floral morph-related genes in heterostylous flax (Linum grandiflorum): the genetic polymorphism and the transcriptional and post-transcriptional regulations of the S locus 査読

    Koichiro Ushijima, Ryohei Nakano, Mayu Bando, Yukari Shigezane, Kazuo Ikeda, Yuko Namba, Saori Kume, Toshiyuki Kitabata, Hitoshi Mori, Yasutaka Kubo

    PLANT JOURNAL   69 ( 2 )   317 - 331   2012年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    Heterostylous species have two types of flowers, thrum and pin morphs, and these are controlled by a single diallelic locus designated the S locus; fertilization between these two types of flowers is successful. The S gene and the molecular mechanism by which it operates remain to be uncovered, although heterostyly has been studied since the time of Darwin. We compared transcripts and proteins of the thrum and pin flowers of heterostylous flax (Linum grandiflorum) to characterize the molecular differences between them and to elucidate the molecular machinery of heterostyly. Twelve floral morph-related genes were eventually isolated by an integrated study of subtraction and 2D-PAGE analyses, and four genes, TSS1, LgAP1, LgMYB21 and LgSKS1, were predicted to be related to heterostyly. TSS1, a thrum style-specific gene, showed some features suitable for the S gene. Although its biological function is unclear, TSS1 was expressed only in the thrum style and is probably linked to the S locus. LgMYB21, another thrum style gene, would be involved in floral morphogenesis. LgMYB21 was highly expressed in the thrum style, which is shorter than the pin style, and its overexpression in Arabidopsis reduced pistil length. Furthermore, a comparison of transcript and protein accumulations showed no differences in the mRNA accumulation of some thrum-specific proteins, including LgSKS1, suggesting that these are regulated by floral morph-specific post-transcriptional regulation. The Linum S locus regulates not only S specificity but also many floral phenotypes. Dynamic regulation of transcripts and proteins would be necessary for the pleiotropic function of the Linum S locus.

    DOI: 10.1111/j.1365-313X.2011.04792.x

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  • Low-temperature-modulated fruit ripening is independent of ethylene in 'Sanuki Gold' kiwifruit 査読

    Eric G. Mworia, Takashi Yoshikawa, Nadiah Salikon, Chisato Oda, William O. Asiche, Naoki Yokotani, Daigo Abe, Koichiro Ushijima, Ryohei Nakano, Yasutaka Kubo

    JOURNAL OF EXPERIMENTAL BOTANY   63 ( 2 )   963 - 971   2012年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Fruit ripening in response to treatments with propylene, 1-methycyclopropene (1-MCP), and low temperature was characterized in 'Sanuki Gold' kiwifruit, Actinidia chinensis Planch. Propylene treatment immediately induced rapid fruit softening, increased AC-PG (polygalacturonase) and AC-EXP (expansin) mRNA accumulation, and stimulated an increase in the soluble solid concentration (SSC) and a decrease in titratable acidity (TA). After 3 d exposure to propylene, ethylene production and AC-PL (pectate lyase) mRNA accumulation were observed. 1-MCP treatment after 24 h exposure to propylene eliminated AC-PG mRNA accumulation and suppressed continued changes in SSC and TA. Application of 1-MCP at the start of the treatment, followed by continuous propylene exposure, markedly delayed fruit softening, and the expression of the cell wall-modifying genes, and changes in the SSC and TA, indicating that kiwifruit become insensitive to ethylene at least for 3 d following 1-MCP exposure. Surprisingly, significant fruit softening, mRNA accumulation of AC-PG, AC-PL, and AC-EXP, and decreased TA were observed without ethylene production in intact fruit stored at low temperature for 1 month, but not in fruit stored at room temperature. Repeated 1-MCP treatments (twice a week) failed to inhibit the changes that occurred in low temperature storage. These observations indicate that low temperature modulates the ripening of kiwifruit in an ethylene-independent manner, suggesting that kiwifruit ripening is inducible by either ethylene or low temperature signals.

    DOI: 10.1093/jxb/err324

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  • The Arabidopsis Na+/H+ Antiporters NHX1 and NHX2 Control Vacuolar pH and K+ Homeostasis to Regulate Growth, Flower Development, and Reproduction (vol 23, pg 3482, 2011) 査読

    Elias Bassil, Hiromi Tajima, Yin-Chih Liang, Masa-aki Ohto, Koichiro Ushijima, Ryohei Nakano, Tomoya Esumi, Ardian Coku, Mark Belmonte, Eduardo Blumwald

    PLANT CELL   23 ( 12 )   4526 - 4526   2011年12月

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    記述言語:英語   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    DOI: 10.1105/tpc.111.231260

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  • Sequence divergence and loss-of-function phenotypes of S locus F-box brothers genes are consistent with non-self recognition by multiple pollen determinants in self-incompatibility of Japanese pear (Pyrus pyrifolia) 査読

    Hiroyuki Kakui, Masaki Kato, Koichiro Ushijima, Miyoko Kitaguchi, Shu Kato, Hidenori Sassa

    PLANT JOURNAL   68 ( 6 )   1028 - 1038   2011年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    The S-RNase-based gametophytic self-incompatibility (SI) of Rosaceae, Solanaceae, and Plantaginaceae is controlled by at least two tightly linked genes located at the complex S locus; the highly polymorphic S-RNase for pistil specificity and the F-box gene (SFB/SLF) for pollen. Self-incompatibility in Prunus (Rosaceae) is considered to represent a self recognition by a single factor system, because loss-of-function of SFB is associated with self-compatibility, and allelic divergence of SFB is high and comparable to that of S-RNase. In contrast, Petunia (Solanaceae) exhibits non-self recognition by multiple factors. However, the distribution of self recognition and non-self recognition SI systems in different taxa is not clear. In addition, in non-self recognition systems, a loss-of-function phenotype of pollen S is unknown. Here we analyze the divergence of SFBB genes, the multiple pollen S candidates, of a rosaceous plant Japanese pear (Pyrus pyrifolia) and show that intrahaplotypic divergence is high and comparable to the allelic diversity of S-RNase while interhaplotypic divergence is very low. Next, we analyzed loss-of-function of the SFBB1 type gene. Genetic analysis showed that pollen with the mutant haplotype S4sm lacking SFBB1-S4 is rejected by pistils with an otherwise compatible S1 while it is accepted by other non-self pistils. We found that the S5 haplotype encodes a truncated SFBB1 protein, even though S5 pollen is accepted normally by pistils with S1 and other non-self haplotypes. These findings suggest that Japanese pear has a non-self recognition by multiple factors SI system, although it is a species of Rosaceae to which Prunus also belongs.

    DOI: 10.1111/j.1365-313X.2011.04752.x

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  • Effect of MA Storage and 1-MCP on Storability and Quality of 'Sanuki Gold' Kiwifruit Harvested at Two Different Maturity Stages 査読

    Eric Gituma Mworia, Takashi Yoshikawa, Nadiah Salikon, Chisato Oda, Tetsuo Fukuda, Katsuhiko Suezawa, William Olubero Asiche, Koichiro Ushijima, Ryohei Nakano, Yasutaka Kubo

    JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE   80 ( 3 )   372 - 377   2011年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC HORTICULTURAL SCI  

    The effects of modified atmosphere (MA) storage and application of 1-methylcyclopropene (1-MCP) at harvest on the storability and quality of 'Sanuki Gold' kiwifruit harvested at two different maturity stages were investigated. MA storage in both fruit harvested early at 136 days after pollination (DAP) or late at 154 DAP delayed flesh softening, increase in soluble solid concentrations (SSC), decrease in titratable acids (TA), and reduction in fruit flesh color index compared to air stored fruit, suggesting that MA storage is effective in prolonging 'Sanuki Gold' kiwifruit storage life. Further, MA stored fruit did not attain full ripening flesh firmness and SSC thresholds even after 4 months of storage under MA conditions, suggesting that early harvested 'Sanuki Gold' kiwifruit can be stored for 4 months in MA. Fruit from both harvesting maturity stages stored under air conditions achieved maximum SSC (18%) values during storage, suggesting that two weeks early harvesting did not compromise edible quality characteristics. Only late harvested fruit treated with 1-MCP and stored in MA showed slight inhibitory effect specific to fruit softening during the first and second month of storage, suggesting that 1-MCP may have some limited ripening inhibitory effect during storage of 'Sanuki Gold' kiwifruit.

    DOI: 10.2503/jjshs1.80.372

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  • Characterization of ethylene biosynthesis and its regulation during fruit ripening in kiwifruit, Actinidia chinensis 'Sanuki Gold' 査読

    Eric G. Mworia, Takashi Yoshikawa, Naoki Yokotani, Tetsuo Fukuda, Katsuhiko Suezawa, Koichiro Ushijima, Ryohei Nakano, Yasutaka Kubo

    POSTHARVEST BIOLOGY AND TECHNOLOGY   55 ( 2 )   108 - 113   2010年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    Ethylene biosynthesis in kiwiftuit, Actinidia chinensis 'Sanuki Gold' was characterized using propylene, an ethylene analog, and 1-methylcyclopropene (1-MCP), an inhibitor of ethylene perception. In fruit harvested between a young stage (66 days after pollination) (DAP) and an early commercial harvesting stage (143 DAP), 2 days of exposure to propylene were sufficient to initiate ethylene biosynthesis while in fruit harvested at commercial harvesting stage (154 DAP), 4 days of propylene treatment were required. This observation suggests that response of ethylene biosynthesis to propylene treatment in kiwifruit declined with fruit maturity. Propylene treatment resulted in up-regulated expression of AC-ACO1, AC-ACO2, AC-SAM1 and AC-SAM2, prior to the induction of AC-ACS1 and ethylene production, confirming that AC-ACS1 is the rate limiting step in ethylene biosynthesis in kiwiftuit. Treatment of fruit with more than 5 mu L L(-1) of 1-MCP after the induction of ethylene production subsequently suppressed ethylene production and expression of ethylene biosynthesis genes. Treatment of fruit with 1-MCP at harvest followed with propylene treatment delayed the induction of ethylene production and AC-ACS1 expression for 5 days. These observations suggest that in ripening kiwifruit, ethylene biosynthesis is regulated by positive feedback mechanism and that 1-MCP treatment at harvest effectively delays ethylene production by 5 days. (C) 2009 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.postharvbio.2009.08.007

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  • Identification and functional analysis of pistil self-incompatibility factor HT-B of Petunia 査読

    Alejandro Raul Puerta, Koichiro Ushijima, Takato Koba, Hidenori Sassa

    JOURNAL OF EXPERIMENTAL BOTANY   60 ( 4 )   1309 - 1318   2009年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Gametophytic self-incompatibility (GSI) in Solanaceae, Rosaceae, and Plantaginaceae is controlled by a multiallelic S-locus. The specificities of pistil and pollen are controlled by separate S-locus genes, S-RNase and SLF/SFB, respectively. Although the S-specificity is determined by the S-locus genes, factors located outside the S-locus are also required for expression of GSI. HT-B is one of the pistil non-S-factors identified in Nicotiana and Solanum, and encodes a small asparagine/aspartate-rich extracellular protein with unknown biochemical function. Here, HT-B was cloned from Petunia and characterized. The structural features and expression pattern of Petunia HT-B were very similar to those of Nicotiana and Solanum. Unlike other solanaceous species, expression of HT-B was also observed in self-compatible Petunia species. RNA interference (RNAi)-mediated suppression of Petunia HT-B resulted in partial breakdown of GSI. Quantitative analysis of the HT-B mRNA accumulation in the transgenics showed that a 100-fold reduction is not sufficient and a > 1000-fold reduction is required to achieve partial breakdown of GSI.

    DOI: 10.1093/jxb/erp005

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  • ナシ果実における二次元電気泳動による細胞壁画分タンパク質の解析

    坂東麻由, 牛島幸一郎, 中野龍平, 稲葉昭次, 久保康隆

    日本食品保蔵科学会誌   34 ( 3 )   127 - 132   2008年5月

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    記述言語:日本語   出版者・発行元:日本食品保蔵科学会  

    セイヨウナシ'ラ・フランス'果実は成熟時に爆発的なエチレン生成と急激な果肉軟化を示す典型的なクライマクテリック型果実である。一方, 同じナシ属に属するチュウゴクナシ品種'ヤーリー'果実は成熟中に多量のエチレンを生成するが軟化しない。本研究では果実軟化の制御にかかわる因子を同定するために, これらナシ果実の細胞壁タンパク質のプロテオーム解析を行った。さまざまな成熟ステージの'ラ・フランス'細胞壁タンパク質を二次元電気泳動で分離したところ, 成熟に伴い変化する19個のタンパク質スポットを得た。これらのうち, エチレン作用阻害剤である1-MCP処理による軟化抑制を行うと, 15個のタンパク質の蓄積がプレクライマクテリック期と同等になった。また, 19個のタンパク質のうち8個が'ヤーリー'果実では成熟に伴って変動せず, 果肉硬度と同じ挙動を示す結果となった。このうち1-MCPにより変動し, 'ヤーリー'では変化しなかったのは2個のスポットである。これら2個のタンパク質の蓄積パターンは果肉軟化様相と同じ挙動を示しており, 軟化の鍵因子の有力な候補と考えられた。

    DOI: 10.5891/jafps.34.127

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  • Differential expression of ethylene biosynthetic genes in climacteric and non-climacteric Chinese pear fruit 査読

    Miki Yamane, Daigo Abe, Sayaka Yasui, Naoki Yokotani, Wataru Kimata, Koichiro Ushijima, Ryohei Nakano, Yasutaka Kubo, Akitsugu Inaba

    POSTHARVEST BIOLOGY AND TECHNOLOGY   44 ( 3 )   220 - 227   2007年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

    We investigated the differences in capability to produce ripening-associated ethylene between climacteric ('Yali', 'Xinqingli', and 'Zhuzuili') and non-climacteric ('Hongli', 'Yuanbali', and 'Hongxiaoli') Chinese pear (Pyrus bretschneideri Rehder) fruit varieties. Three ACS (PbACS1, PbACS2, and PbACS3), two ACO (PbACO1 and PbACO2), and three MADS-box (PbMADS1, PbMADS2, and PbMADS3) genes were cloned from ripening fruit. Fruit were harvested at the mature stage and treated with 5000 mu L L-1 propylene for 4 days. Ethylene production was induced by propylene in the climacteric but not in non-climacteric type fruit. In the ripening climacteric fruit, PbACS1 and PbACO1 transcript accumulation accompanied ethylene production but the accumulation of other ACS and ACO mRNAs was not detected. In 'Yali' fruit, 1-MCP exposure prior to propylene treatment completely inhibited the expression of these genes, while exposure after the commencement of ethylene production weakened their expression. Transcripts of PbACO1 accumulated in response to propylene treatment even in non-climacteric fruit but this accumulation was eliminated after the termination of propylene treatment. In response to wounding, transcripts of PbACS2, PbACS3, and PbACO2 genes accumulated in both climacteric and non-climacteric fruit, but accumulation of PbACS1 and PbACO1 mRNAs was not detected. In the Southern analysis of PbACS1, HindIII digests of genomic DNA showed 8.3, 3.5 and 2.9 kb bands. The 2.9 kb band was detected only in climacteric varieties while the 3.5 kb band was detected in both climacteric and non-climacteric varieties except in 'Yali'. These results suggest that Chinese pears may have two copies of the ACSI gene, in which PbACS1A could be linked to the varietal differences in the capability to produce ripening-associated ethylene. There was no correlation between the expression patterns of the three MADS-box genes cloned and the differences in ripening-associated ethylene production among the Chinese pear varieties. (C) 2006 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.postharvbio.2006.12.010

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  • S locus F-Box brothers: Multiple and pollen-specific F-box genes with S haplotype-specific polymorphisms in apple and Japanese pear 査読

    Hidenori Sassa, Hiroyuki Kakui, Mayu Miyamoto, Yusuke Suzuki, Toshio Hanada, Koichiro Ushijima, Makoto Kusaba, Hisashi Hirano, Takato Koba

    GENETICS   175 ( 4 )   1869 - 1881   2007年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:GENETICS  

    Although recent findings suggest that the F-box genes SFB/SLF control pollen-part S specificity in the S-RNase-based gametophytic self-incompatibility (GSI) system, how these genes operate in the system is unknown, and functional variation of pollen S genes in different species has been reported. Here, we analyzed the S locus of two species of Maloideae: apple (Malus domestica) and Japanese pear (Pyrus pyrifolia). The sequencing of a 317-kb region of the apple S-9 haplotype revealed two similar F-box genes. Homologous sequences were isolated from different haplotypies of apple and Japanese pear, and they were found to be polymorphic genes derived from the Slocus. Since each S haplotype contains two or three related genes, the genes were named SFBB for S locus F-box brothers. The SFBB genes are specifically expressed in pollen, and variable regions of the SFBB genes are under positive selection. In a style-specific mutant S haplotype of Japanese pear, the SFBB genes are retained. Apart from their multiplicity, SFBB genes meet the expected characteristics of pollen S. The unique multiplicity of SFBB genes as the pollen S candidate is discussed in the context of mechanistic variation in the S-RNase-based GSI system.

    DOI: 10.1534/genetics.106.068858

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  • beta-galactosidase and alpha-L-arabinofuranosidase activities and gene expression in European and Chinese pear fruit during ripening 査読

    Mercy W. Mwaniki, Francis M. Mathooko, Kyoko Hiwasa, Akira Tateishi, Naoki Yokotani, Koichiro Ushijima, Ryohei Nakano, Akitsugu Inaba, Yasutaka Kubo

    JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE   76 ( 1 )   85 - 90   2007年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPAN SOC HORTICULTURAL SCI  

    Substantial decreases in cell wall bound galactosyl and arabinosyl residues are two of the most evident cell wall compositional changes that occur during fruit ripening. The roles of P-galactosidase (P-Gal) and alpha-L-arabinofuranosidase (alpha-Af), the enzymes responsible for these respective losses, were investigated and compared in European (Pyrus communis L. 'La France') and Chinese (Pyrus bretschneideri Rehd. 'Yali') pear fruits which exhibit different softening characteristics during ripening. The increase in the activities of P-Gal and a-Af during ripening in both types of pear fruit correlated well with an increase in climacteric ethylene production, and a concomitant decrease in flesh firmness in 'La France' fruit. However, there was no noticeable decrease in 'Yali' flesh firmness even after 28 days of storage at room temperature. In both fruit types, enzyme activity and the accumulation of transcripts hybridizing with PpGAL1, PpGAL4, PpARF2, and PcARF1 increased with fruit ripening. Increases in gene expression and enzyme activities in 'Yali' fruit with no detectable softening during ripening indicate that P-Gal and a-Af may not mediate difference in fruit softening between two pears, but that they could play some role(s) in cell wall changes, perhaps in cooperation with other cell wall-modifying enzymes such as polygalacturonase.

    DOI: 10.2503/jjshs.76.85

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  • Ethylene regulation of fruit softening and cell wall disassembly in Charentais melon 査読

    Kiyomi Nishiyama, Monique Guis, Jocelyn K. C. Rose, Yasutaka Kubo, Kristen A. Bennett, Wangjin Lu, Kenji Kato, Koichiro Ushijima, Ryohei Nakano, Akitsugu Inaba, Mondher Bouzayen, Alain Latche, Jean-Claude Pech, Alan B. Bennett

    JOURNAL OF EXPERIMENTAL BOTANY   58 ( 6 )   1281 - 1290   2007年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

    Cell wall disassembly in ripening fruit is highly complex, involving the dismantling of multiple polysaccharide networks by diverse families of wall-modifying proteins. While it has been reported in several species that multiple members of each such family are expressed in the same fruit tissue, it is not clear whether this reflects functional redundancy, with protein isozymes from a single enzyme class performing similar roles and contributing equally to wall degradation, or whether they have discrete functions, with some isoforms playing a predominant role. Experiments reported here sought to distinguish between cell wall-related processes in ripening melon that were softening-associated and softening-independent. Cell wall polysaccharide depolymerization and the expression of wall metabolism-related genes were examined in transgenic melon (Cucumis melo var. cantalupensis Naud.) fruit with suppressed expression of the 1-aminocyclopropane-1-carboxylate oxidase (ACO) gene and fruits treated with ethylene and 1-methylcyclopropene (1-MCP). Softening was completely inhibited in the transgenic fruit but was restored by treatment with exogenous ethylene. Moreover, post-harvest application of 1-MCP after the onset of ripening completely halted subsequent softening, suggesting that melon fruit softening is ethylene-dependent. Size exclusion chromatography of cell wall polysaccharides, from the transgenic fruits, with or without exogenous ethylene, indicated that the depolymerization of both pectins and xyloglucans was also ethylene dependent. However, northern analyses of a diverse range of cell wall-related genes, including those for polygalacturonases, xyloglucan endotransglucosylase/hydrolases, expansin, and beta-galactosidases, identified specific genes within single families that could be categorized as ethylene-dependent, ethylene-independent, or partially ethylene-dependent. These results support the hypothesis that while individual cell wall-modifying proteins from each family contribute to cell wall disassembly that accompanies fruit softening, other closely related family members are regulated in an ethylene-independent manner and apparently do not directly participate in fruit softening.

    DOI: 10.1093/jxb/erl283

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  • 極晩生モモにおける果実肥大および成熟様相

    福田 文夫, 玉木 由佳, 河井 崇, 牛島 幸一郎, 平野 健, 小田 賢司, 原 美由紀, 深松 陽介, 森永 邦久, 中野 龍平

    園芸学研究 = Horticultural research (Japan)   20 ( 1 )   65 - 71   2021年1月

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    記述言語:日本語   出版者・発行元:園芸学会  

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  • モモ‘冬桃がたり’の極晩生成熟特性のF1後代における遺伝様式

    河井崇, 牛島幸一郎, 三宅春菜, 草加芽依, 深松陽介, 原美由紀, 鵜木悠治郎, 日原誠介, 中野龍平, 小田賢司, 福田文夫

    園芸学研究 別冊   19 ( 1 )   2020年

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  • マメガキを用いたカキの甘渋性決定遺伝子座の比較ゲノム解析

    西山総一郎, 佐藤明彦, 河野淳, 尾上典之, 牛島幸一郎, 山根久代, 田尾龍太郎, 米森敬三

    園芸学研究 別冊   14 ( 2 )   2015年

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  • 甘果オウトウS遺伝子座における遺伝的組換の抑制

    池田 和生, 牛島 幸一郎, 山根 久代, IEZZONI Amy, 田尾 龍太郎

    園芸学会雑誌. 別冊, 園芸学会大会研究発表   73 ( 2 )   2004年9月

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    記述言語:日本語  

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  • ウメの自家和合性S^fハプロタイプの解析

    筧 永子, 牛島 幸一郎, 山根 久代, 田尾 龍太郎

    園芸学会雑誌. 別冊, 園芸学会大会研究発表   73 ( 2 )   2004年9月

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    記述言語:日本語  

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  • Prunus 属果樹のSFBの一次構造の特徴

    池田 和生, 牛島 幸一郎, 山根 久代, 佐々 英徳, IEZZONI A. F, KOHN J. R, 田尾 龍太郎

    園芸学会雑誌. 別冊, 園芸学会大会研究発表   73 ( 1 )   2004年4月

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  • 甘果オウトウの自家和合性S^4'ハプロタイプの解析 : (第1報)DNAブロット分析によるSハプロタイプ特異的Fボックスタンパク質遺伝子(SFB)の解析

    池田 和生, 渡 晶子, 牛島 幸一郎, 山根 久代, IEZZONI A. F, 田尾 龍太郎

    園芸学会雑誌. 別冊, 園芸学会大会研究発表   72 ( 2 )   2003年9月

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  • Prunus 属のS遺伝子座の構造的特徴

    牛島 幸一郎, 佐々 英徳, 山根 久代, 田尾 龍太郎

    園芸学会雑誌. 別冊, 園芸学会大会研究発表   72 ( 1 )   2003年4月

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