Faculty Profiles - HANAFUSA Tadashi

Papers - HANAFUSA Tadashi

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Promoter-independent loss of mRNA and protein of the Rb gene in a human hepatocellular carcinoma Reviewed

H Hada, N Koide, T Morita, H Shiraha, T Shinji, M Nakamura, K Ujike, N Takayama, T Oka, T Hanafusa, Y Yumoto, K Hamazaki, T Tsuji

HEPATO-GASTROENTEROLOGY 43 ( 11 ) 1185 - 1189 1996.9

Language：English Publishing type：Research paper (scientific journal) Publisher：H G E UPDATE MEDICAL PUBL LTD.

Background: Inactivation of the retinoblastoma (Rb) gene is considered to play a fundamental role in the genesis and progression of several human cancers. In retinoblastoma, the inactivation of Rb promoter by mutations or hypermethylation has been reported. Although genetic changes of Rb gene have been described in hepatocellular carcinoma (HCC) an, epigenetic change such as hypermethylation of the Rb promoter as reported in, retinoblastoma has not been described.
Materials and Methods: We examined the hypermethylation in the promoter region of Rb gene by restriction fragment length polymorphism in 19 HCCs, as well as the expression of Rb mRNA and protein by RT-PCR and by immunoblotting, respectively.
Results: We found no evidence of hypermethylation. in the promoter region, of the Rb gene in all HCCs analyzed. However, the expression of Rb mRNA and protein was lost in one HCC, and no mutation was detected in the Rb promoter region of this patient. The inactivation of Rb promoter by hypermethylation or by inhibition of binding of transcription factors due to point mutations did not contribute to the loss of mRNA and protein in. the patient.
Conclusions: Hypermethylation in the Rb promoter region appeared to have little causal effect on HCC.

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Loss of heterozygosity and analysis od mutation of p53 in hepatocellular carcinoma (jointly worked) Reviewed

Yumoto Y, Hanafusa T, Hada H, Morita T, Ooguch S, Shinji T, Mitani T, Hamaya K, Koide N, Tsuji T

Journal of Gastroenterology and Hepatology 10 179 - 185 1995.4

Classification of hepatitis C viras into subgroups on the basis of Sequence variations in the envelope protein (jointly worked) Reviewed

Hada H, Koide N, Hanafusa T, Takabatake H, Sakaguchi K, Shinji T, Sasaki S, Oka T, Takayama N, Yumoto Y, and Tsuji T

Journal of Gastroenterology and Hepatology 8,70-74 70 - 74 1993.4

Isolation and characterization of DBEscherichia(/)-DB DBcoli(/)-DB DBhag(/)-DB operator mutants whose hag-48 expression has become repressible by a DBSalmonella(/)-DB DBHl(/)-DB repressor (jointly worked) Reviewed

Hanafusa T, Sakai A, Tominaga A, Enomoto M

Mol. Gen. Genet. 216 1993.4

Nucleotide sequence and regulated expression of the Salmonella fljA gene encoding a repressor of the phase 1 flagellin gene Reviewed

Tadashi Hanafusa, Kenji Saito, Akira Tominaga, Masatoshi Enomoto

MGG Molecular & General Genetics 236 ( 2-3 ) 260 - 266 1993.1

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：Springer-Verlag

The nucleotide sequence of Salmonella abortus-equi fljA, which together with the phase 2 flagellin gene constitutes the fljBA operon and encodes the repressor for the phase 1 flagellin gene fliC, was determined. The repressor was predicted to be a basic protein consisting of 179 amino acid residues (Mr = 20419 Da) encoded by ORFII. This was confirmed by the fact that host fliC is repressed by plasmid-encoded ORFII, which indeed expresses a 20 kDa product as determined by urea SDS-polyacrylamide gel electrophoresis. An amino acid sequence capable of forming a helix-turn-helix type of structure was predicted in the C-terminal region of FljA. A rho-independent intercistronic terminator was detected between fljB and ftjA. Chloramphenicol acetyltransferase (CAT) assays of fusions indicated that the terminator is capable of reducing expression of fljA to the level of a few percent, relative to fljB in broth cultures and to 1 % in M9 glycerol cultures. © 1993 Springer-Verlag.

DOI： 10.1007/BF00277121

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Integration of SCPl, a giant linear plasmid, into the DBStreptomyces(/)-DB DBcoelicolor(/)-DB chromosome (jointly worked) Reviewed

Kinashi H, Shimaji-Murayama M and Hanafusa T

Gene 115 35 - 41 1992.4

The structure of an integrated copy of the giant linear plasmid SCPl in the chromosome of DBStreptomyces(/)-DB DBcoelicolor(/)-DB 2612 (jointly worked) Reviewed

HANAFUSA T.

Mol. Gen. Genet. 231 363 - 368 1992.4

Detection by western blotting of an antibody to the hepatitis C virus E1 envelope protein in sera of patients with chronic liver disease.

H. Hada, N. Koide, T. Hanafusa, K. Sakaguchi, T. Shinji, S. Sasaki, T. Oka, N. Takayama, Y. Yumoto, T. Tsuji

Acta medica Okayama 46 ( 5 ) 365 - 370 1992

Language：English Publishing type：Research paper (scientific journal)

We detected an antibody to HCV envelope protein (E1) in sera of patients with HCV-related chronic liver diseases (20 patients with chronic hepatitis and 5 patients with liver cirrhosis) by Western blotting using the fusion protein of E1 envelope protein and beta-galactosidase as an antigen. The antibody to HCV E1 (anti-HCV E1) was detected in 8 (42%) of 19 patients positive for HCV-RNA (16 were positive and 3 were negative for antibody to C100-3) and in 1 (17%) of 6 patients negative for HCV-RNA but positive for antibody to C100-3. HCV-RNA was detected in 8 (89%) of 9 anti-HCV E1 positive sera. The value of alanine aminotransferase was significantly higher in patients positive for anti-HCV E1 than in patients negative for the antibody. Although an antibody to the envelope protein of HCV is suspected to be one of the candidates of virus-neutralizing antibodies, our results suggest this hypothesis appears to be unlikely.

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Sequence Variations in the Envelope Protein of the Hepatitis C Virus : Comparison with Partial cDNA Sequence of a New Variant Virus Obtained by the Polymerase Chain Reaction (jointly worked) Reviewed

Hada H, Koide N, Takabatake H, Hanafusa T and Tsuji T

Acta Med Okayama 45 ( 5 ) 347 - 355 1991.4

Nucleotide sequence analysis of the unusually long terminal inverted repeats of a giant linear plasmid, SCPl (jointly worked) Reviewed

Kinashi H, Shimaji-Murayama M and Hanafusa T

Plasmid 26 123 - 130 1991.4

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