2021/07/12 更新

写真a

ホソヤ オサム
細谷 修
HOSOYA Osamu
所属
医歯薬学域 助教
職名
助教
外部リンク

学位

  • 博士(理学) ( 新潟大学 )

研究キーワード

  • 細胞生物学

  • Cell Biology

研究分野

  • ライフサイエンス / 神経科学一般

  • ライフサイエンス / 細胞生物学

  • ライフサイエンス / 神経科学一般

学歴

  • 新潟大学   Graduate School, Division of Natural Science  

    - 1998年

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  • 新潟大学    

    - 1998年

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    国名: 日本国

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  • 新潟大学   理学部   生物学科

    - 1993年

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    国名: 日本国

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  • 新潟大学   Faculty of Science  

    - 1993年

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経歴

  • - 岡山大学医歯薬学総合研究科生体制御科学専攻 助教

    2004年

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  • - Assistant Professor,Dept. Neurogenomics, Grad. Sch. Med. Dent. Pharm. Sci., Okayama Univ.,Biophysiological Sciences,Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,Okayama University

    2004年

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所属学協会

 

MISC

  • Vision maintenance and retinal apoptosis reduction in RCS rats with Okayama University-type retinal prosthesis (OUReP (TM)) implantation

    Alamusi, Toshihiko Matsuo, Osamu Hosoya, Kimiko M. Tsutsui, Tetsuya Uchida

    JOURNAL OF ARTIFICIAL ORGANS   18 ( 3 )   264 - 271   2015年9月

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    記述言語:英語   出版者・発行元:SPRINGER JAPAN KK  

    Photoelectric dye-coupled polyethylene film, designated Okayama University-type retinal prosthesis or OUReP (TM), generates light-evoked surface electric potentials and stimulates neurons. In this study, the vision was assessed by behavior tests in aged hereditary retinal dystrophic RCS rats with OUReP (TM), retinal apoptosis and electroretinographic responses were measured in dystrophic eyes with OUReP (TM). The dye-coupled films, or plain films as a control, were implanted in subretinal space of RCS rats. On behavior tests, RCS rats with dye-coupled films, implanted at the old age of 14 weeks, showed the larger number of head-turning, consistent with clockwise and anticlockwise rotation of a surrounding black-and-white-striped drum, compared with rats with plain films, under the dim (50 lux) and bright (150 lux) conditions in the observation period until the age of 22 weeks (n = 5, P < 0.05, repeated-measure ANOVA). The number of apoptotic cells in retinal sections at the site of dye-coupled film implantation was significantly smaller, compared with the other retinal sites, neighboring the film, or opposite to the film, 5 months after film implantation at the age of 6 weeks (P = 0.0021, Friedman test). The dystrophic eyes of RCS rats with dye-coupled films showed positive responses to maximal light stimulus at a significantly higher rate, compared with the eyes with no treatment (P < 0.05, Chi-square test). Electroretinograms in normal eyes of Wistar rats with dye-coupled or plain films showed significantly decreased amplitudes (n = 14, P < 0.05, repeated-measure ANOVA). In conclusions, vision was maintained in RCS rats with dye-coupled films implanted at the old age. The dystrophic eyes with dye-coupled films showed electroretinographic responses. Five-month film implantation caused no additional retinal changes.

    DOI: 10.1007/s10047-015-0825-1

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  • 精神・神経疾患に関連する長大遺伝子の発現制御機構

    宮地まり, 佐野訓明, 古田良平, 細谷修, 筒井公子, 筒井研

    月刊細胞   47 ( 5 )   25 - 28   2015年

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  • Nuclear dynamics of topoisomerase IIβ reflects its catalytic activity that is regulated by binding of RNA to the C-terminal domain

    A. Onoda, O. Hosoya, K. Sano, K. Kiyama, H. Kimura, S. Kawano, R. Furuta, M. Miyaji, K. Tsutusi, K.M. Tsutsui

    Nucleic Acids Research   42 ( 14 )   9005 - 9020   2014年

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  • Behavior tests and immunohistochemical retinal response analyses in RSC rats with subretinal implantation of Okayama-University-type retinal prosthesis (共著)

    Alamusi, Matsuo T, Hosoya O, Tsutsui KM, Uchida T

    Journal of Artificial Organs   16 ( 3 )   343 - 351   2013年9月

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  • Nuclear protein LEDGF/p75 recognizes supercoiled DNA by a novel DNA-binding domain

    Kimiko M. Tsutsui, Kuniaki Sano, Osamu Hosoya, Tadashi Miyamoto, Ken Tsutsui

    NUCLEIC ACIDS RESEARCH   39 ( 12 )   5067 - 5081   2011年7月

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    記述言語:英語   出版者・発行元:OXFORD UNIV PRESS  

    Lens epithelium-derived growth factor (LEDGF) or p75 is a co-activator of general transcription and also involved in insertion of human immunodeficiency virus type I (HIV-1) cDNA into host cell genome, which occurs preferentially to active transcription units. These phenomena may share an underlying molecular mechanism in common. We report here that LEDGF/p75 binds negatively supercoiled DNA selectively over unconstrained DNA. We identified a novel DNA-binding domain in the protein and termed it 'supercoiled DNA-recognition domain' (SRD). Recombinant protein fragments containing SRD showed a preferential binding to supercoiled DNA in vitro. SRD harbors a characteristic cluster of lysine and glutamic/aspartic acid residues. A polypeptide mimicking the cluster (K(9)E(9)K(9)) also showed this specificity, suggesting that the cluster is an essential element for the supercoil recognition. eGFP-tagged LEDGF/p75 expressed in the nucleus distributed partially in transcriptionally active regions that were identified by immunostaining of methylated histone H3 (H3K4me3) or incorporation of Br-UTP. This pattern of localization was observed with SRD alone but abolished if the protein lacked SRD. Thus, these results imply that LEDGF/p75 guides its binding partners, including HIV-1 integrase, to the active transcription site through recognition of negative supercoils generated around it.

    DOI: 10.1093/nar/gkr088

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  • Glial reaction to photoelectric dye-based retinal prostheses implanted in the subretinal space of rats

    Takayuki Tamaki, Toshihiko Matsuo, Osamu Hosoya, Kimiko M. Tsutsui, Tetsuya Uchida, Kazuo Okamoto, Akihito Uji, Hiroshi Ohtsuki

    JOURNAL OF ARTIFICIAL ORGANS   11 ( 1 )   38 - 44   2008年4月

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    記述言語:英語   出版者・発行元:SPRINGER TOKYO  

    We have designed a new type of retinal prosthesis using polyethylene films coupled with photoelectric dye molecules that absorb light and convert photon energy to electric potentials. An extruded-blown film of high-density polyethylene was used as the original polyethylene film. Recrystallized film was made by recrystallization from the melting of the original polyethylene film. A photoelectric dye,2-[2-[4-(dibutylamino)phenyl]ethenyl]-3-carboxymethylbenzothiazolium bromide, was coupled to the two types of polyethylene films through amide linkages. Samples of the original dye-coupled film, the dye-coupled recrystallized film, and the dye-uncoupled plain film were implanted in the subretinal space of normal adult rats. Frozen sections were cut from the eyes enucleated at 1 week or 1 month and were either stained with hematoxylin and eosin, stained immunohistochemically for glial fibrillary acidic protein (GFAP), or processed for in situ apoptosis detection. The results revealed that retinal tissue damage was negligible with no inflammatory cells and few apoptotic cells. GFAP was significantly up-regulated in retinal sites with the implantation of all types of polyethylene films at 1 week, compared with the adjacent retinal sites (P < 0.005, analysis of variance). The GFAP up-regulation was also present at 1 month for the plain film and dye-coupled recrystallized film (P < 0.05). Glial cell encirclement around the films increased significantly between 1 week and 1 month (P = 0.023, two-factor analysis of variance) but was not significantly different among the three types of polyethylene films (P = 0.4531). These results showed evidence of glial reactions to the photoelectric dye-coupled polyethylene films implanted into the subretinal space of rat eyes and also proved their basic biological safety.

    DOI: 10.1007/s10047-007-0398-8

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  • Amphiphysin Ir participates in receptor-mediated endocytosis in ARPE-19

    Osamu Hosoya, Ken Tsutsui, Kimiko Tsutsui

    Neuroscience Research Supplement   58/S1 S95   2007年

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  • Expression dynamics and functional implications of DNA topoisomeraseⅡβ in the brain

    Tsutsui K M, Sano K, Hosoya O, Tsusui K

    Anatomical Science International   81 ( 3 )   156 - 163   2006年9月

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    記述言語:英語   掲載種別:書評論文,書評,文献紹介等  

    DOI: 10.1111/j.1447-073x.2006.00146.x

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  • Expression dynamics and functional implications of DNA topoisomerase II beta in the brain

    Kimiko Tsutsui, Kuniaki Sano, Osamu Hosoya, Ken Tsutsui

    Anatomical Science International   2006年

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  • Subcellular localization of amphiphysin Ir in ARPE-19 cells

    Osamu Hosoya, Ken Tsutsui, Kimiko Tsutsui

    Neuroscience Research   2006年

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  • Localized expression of amphiphysin Ir, a retina-specific variant of amphiphysin I, in the ribbon synapse and its functional implication

    O Hosoya, K Tsutsui, K Tsutsui

    EUROPEAN JOURNAL OF NEUROSCIENCE   19 ( 8 )   2179 - 2187   2004年4月

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    記述言語:英語   出版者・発行元:WILEY-BLACKWELL  

    In the vertebrate retina, presynaptic terminals of photoreceptors and bipolar cells form ribbon synapses and release neurotransmitter continuously. Endocytic machinery in the ribbon synapse is likely to differ from that in conventional synapses because of the much higher rate of synaptic vesicle recycling. However, protein components of the ribbon synapse identified so far are quite similar to those of the conventional synapses. Recently we identified amphiphysin I splice variants, termed amphiphysin Ir, that are transcribed specifically in retina from the authentic amphiphysin I gene [Y Terada et al. (2002) FEBS Lett., 519, 185-190]. Amphiphysin I is a nerve terminal-enriched protein, and involved in synaptic vesicle endocytosis as heterodimer with amphiphysin II, an isoform of amphiphysin I. We report here that the retina-specific amphiphysin Ir is expressed exclusively in the ribbon synapse and not in conventional synapses. This is the first endocytosis-related, ribbon synapse-specific protein identified in the retina. By immunoprecipitation and double-immunolabelling, amphiphysin Ir was shown to be associated not only with amphiphysin 11, but also with dynamin, clathrin and alpha-adaptin that are involved in synaptic vesicle recycling. The results suggest that endocytosis of the synaptic vesicle membrane in retinal ribbon synapses proceeds through a pathway similar to the one that is used in conventional synapses, although amphiphysin Ir is substituted for amphiphysin I. Amphiphysin Ir may play an essential role in the avid endocytic activity of ribbon synapses by associating with yet unknown protein partner(s) through its large insertional domain, which is absent from the conventional amphiphysin I.

    DOI: 10.1111/j.1460-9568.2004.03340.x

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  • Elucidating the role of amphiphysin Ir in retinal ribbon synapses

    Osamu Hosoya, Ken Tsutsui, Kimiko Tsutsui

    Neuroscience research   2003年

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  • Localization of dynamin 2 in rat seminiferous tubules during the spermatogenic cycle

    H Iguchi, M Watanabe, A Kamitani, A Nagai, O Hosoya, K Tsutsui, H Kumon

    ACTA MEDICA OKAYAMA   56 ( 4 )   205 - 209   2002年8月

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    記述言語:英語   出版者・発行元:OKAYAMA UNIV MED SCHOOL  

    Dynamin is a protein essential to endocytosis. Dynamin 2, a dynamin isoform, is expressed most intensely in testicular tissue; however, precise localization has never been studied. Therefore, we investigated the expression of dynamin 2 in rat testicular tissue using immunohistochemical methods, and discuss here the physiological function of this protein. Testicular tissues were obtained from Wistar rats at 10, 21 and 63 days of age. Immunohistochemistrical examination and Western blot analysis were conducted using dynamin 2 specific antibody. Western blot analysis showed that expression in 21- and 63-day-old rats was more intense than that in 10-day-old rats. Dynamin 2 expression was observed using immunohistochemical method in the seminiferous tubules of all rats. In the 63-day-old rats, the expression was intense, especially in spermatids in the earlier maturation stages and in spermatocytes, and was observed in Sertoli cells. However, in spermatids, the expression gradually declined as spermatids matured to spermatozoa. In the 21-day-old rats, the expression was evident in spermatocytes and Sertoli cells, but that in the 10-day-old rats was weak. Intense expression of dynamin 2 during spermatogenesis suggests that this protein plays an important role in this process.

    DOI: 10.18926/AMO/31681

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  • Novel splice variants of amphiphysin I are expressed in retina

    Y Terada, K Tsutsui, K Sano, O Hosoya, H Ohtsuki, A Tokunaga, K Tsutsui

    FEBS LETTERS   519 ( 1-3 )   185 - 190   2002年5月

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    記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

    Using RT-PCR-based cDNA cloning, we identified novel splice variants of amphiphysin I, termed amph Ir, that are expressed specifically in retina. In comparison with the prototype amphiphysin I, amph Ir contained two novel insertions (inserts A and B) and one deletion. Insert A is only 9 bp in length but appears to be a determinant for the retina-specific expression. In contrast, insert B is a large domain of 1740 bp and two shorter transcripts with 3'-truncated insert B were also expressed. All the insert sequences were present as unidentified exons in the amphiphysin I gene on human chromosome 7. Western blot analysis of various rat tissues with anti-insert B antibody confirmed the presence and tissue specificity of the variant proteins. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

    DOI: 10.1016/S0014-5793(02)02763-1

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  • Localization and functional role of amphiphysin Ir in retinal ribbon synapses.

    Hosoya O, Tsutsui K, Sano K, Tsutsui K

    Neurosci Res Suppl   26/S37   2002年

  • Expression of amphiphysin I in sertoli cells and its implication in spermatogenesis

    M Watanabe, K Tsutsui, O Hosoya, K Tsutsui, H Kumon, A Tokunaga

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   287 ( 3 )   739 - 745   2001年9月

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    記述言語:英語   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Amphiphysin I is a protein concentrated in nerve terminals and involved in the endocytosis of synaptic vesicle membrane. We show here that amphiphysin I is expressed in the rat testis, localized exclusively in the Sertoli cells. In the postnatal testicular development, expression of amphiphysin I was not evident at birth, but became significant at postnatal day 15 (P15), coinciding with the onset of spermatogenesis. The expression level of amphiphysin I increased 10-fold between P15 and P25 to reach the adult level. In adult testes reversibly damaged by ethane dimethane sulphonate administration, expression of amphiphysin I did not change following the damage, whereas the protein was transiently converted into its phosphorylated form. The increase in levels of phosphorylated amphiphysin I was closely associated with the severe histological damage to germ cells. The present findings suggest that amphiphysin I in Sertoli cells is involved in spermatogenesis, probably through endocytic processes. (C) 2001 Academic Press.

    DOI: 10.1006/bbrc.2001.5650

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  • Expression of amphiphysin I in sertoli cells and its implication in spermatogenesis

    M Watanabe, K Tsutsui, O Hosoya, K Tsutsui, H Kumon, A Tokunaga

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   287 ( 3 )   739 - 745   2001年9月

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    記述言語:英語   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Amphiphysin I is a protein concentrated in nerve terminals and involved in the endocytosis of synaptic vesicle membrane. We show here that amphiphysin I is expressed in the rat testis, localized exclusively in the Sertoli cells. In the postnatal testicular development, expression of amphiphysin I was not evident at birth, but became significant at postnatal day 15 (P15), coinciding with the onset of spermatogenesis. The expression level of amphiphysin I increased 10-fold between P15 and P25 to reach the adult level. In adult testes reversibly damaged by ethane dimethane sulphonate administration, expression of amphiphysin I did not change following the damage, whereas the protein was transiently converted into its phosphorylated form. The increase in levels of phosphorylated amphiphysin I was closely associated with the severe histological damage to germ cells. The present findings suggest that amphiphysin I in Sertoli cells is involved in spermatogenesis, probably through endocytic processes. (C) 2001 Academic Press.

    DOI: 10.1006/bbrc.2001.5650

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  • Immnnohistochemical Analyses of DNA Topoisomerase (]G0002[) isoforms in Developing Rat Cerebellum

    Kimiko Tsutsui, Ken Tsutsui, Osamu Hosoya, Kuniaki Sano, Akira Tokunaga

    The Journal of Comparative Neurology   431 ( 2 )   228 - 239   2001年

  • Immnnohistochemical Analyses of DNA Topoisomerase (]G0002[) isoforms in Developing Rat Cerebellum

    The Journal of Comparative Neurology   431 ( 2 )   228 - 239   2001年

  • Immunocytochemical studies on the cellular origin of ectopic striated muscle fibers in monolayer cultures of rat anterior pituitary cells

    O Hosoya, YG Watanabe

    ZOOLOGICAL SCIENCE   15 ( 2 )   247 - 253   1998年4月

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    記述言語:英語   出版者・発行元:ZOOLOGICAL SOC JAPAN  

    We have previously reported that glia-like folliculo-stellate (FS) cells in the rat anterior pituitary may be involved in heterotopic differentiation of striated muscles which appear during pituitary cell culture. In the present immunocytochemical study, cytological alterations of FS cells in vitro were investigated by using antibodies to marker proteins (i.e., S-100 and vimentin) for FS cells. Expression of skeletal muscle-specific MyoD1 detected by immunocytochemistry, enabled identification of myogenic cells at a stage when they were still unicellular. Elongated myoblasts containing MyoD1-positive nuclei were found as early as the fifth day of monolayer culture of pituitary cells. The double immunostaining technique showed that some of these myoblasts reacted with antiserum to S-100. Although all of the myoblasts were immunoreactive to vimentin, this marker protein was unable to identify FS cells in vitro because rapidly proliferating fibroblasts were also immunoreactive to vimentin. Since the antiserum to S-100 that we used did not react with already differentiated striated muscle fibers, the demonstration of both MyoD1 and S-100-immunoreactive myoblasts in our pituitary cultures suggests that these myogenic cells are derived from FS cells. The present study does not rule out the possibility that fibroblasts, whose origin is presently unknown, are also involved in myogenesis in pituitary cultures.

    DOI: 10.2108/zsj.15.247

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  • Immunocytochemical studies on the cellular origin of ectopic striated muscle fibers in monolayer cultures of rat anterior pituitary cells

    Osamu Hosoya, Yuichi G. Watanabe

    Zoological Science   15 ( 2 )   247 - 253   1998年

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    記述言語:英語   出版者・発行元:Zoological Society of Japan  

    We have previously reported that glia-like folliculo-stellate (FS) cells in the rat anterior pituitary may be involved in heterotopic differentiation of striated muscles which appear during pituitary cell culture. In the present immunocytochemical study, cytological alterations of FS cells in vitro were investigated by using antibodies to marker proteins (i.e., S-100 and vimentin) for FS cells. Expression of skeletal muscle-specific MyoD1 detected by immunocytochemistry, enabled identification of myogenic cells at a stage when they were still unicellular. Elongated myoblasts containing MyoD1-positive nuclei were found as early as the fifth day of monolayer culture of pituitary cells. The double immunostaining technique showed that some of these myoblasts reacted with antiserum to S-100. Although all of the myoblasts were immunoreactive to vimentin, this marker protein was unable to identify FS cells in vitro because rapidly proliferating fibroblasts were also immunoreactive to vimentin. Since the antiserum to S-100 that we used did not react with already differentiated striated muscle fibers, the demonstration of both MyoD1 and S-100-immunoreactive myoblasts in our pituitary cultures suggests that these myogenic cells are derived from FS cells. The present study does not rule out the possibility that fibroblasts, whose origin is presently unknown, are also involved in myogenesis in pituitary cultures.

    DOI: 10.2108/zsj.15.247

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  • Intersexual differences and seasonal changes in gonadotropin-producing cells in the salamander Hynobius nigrescens

    M Hasumi, S Sasaki, O Hosoya, K Sato, H Haraguchi

    JOURNAL OF HERPETOLOGY   31 ( 1 )   45 - 51   1997年3月

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    記述言語:英語   出版者・発行元:SOC STUDY AMPHIBIANS REPTILES  

    Intersexual differences and annual cytological changes in presumptive gonadotropin-producing cells (gonadotropes) were examined immunohistochemically in mid-sagittal sections of the pituitary gland of adult Hynobius nigrescens by the use of a monoclonal antibody against bullfrog luteinizing hormone beta-subunit (LH beta). Total immunoreactive areas for LH beta and profiles of the pars distalis in the mid-sagittal plane of the gland were estimated with an image analyzer. Gonadotropes consistently were distributed in the pars distalis near the median eminence and the pars intermedia in both sexes throughout the year; an intersexual difference was found in their distribution pattern. Gonadotrope cells other than these consistent ones distributed in the peripheral region of the pars distalis frequently were observed in females, but not in males. The proportion of gonadotropes among all pars distalis cells, as well as the mean area and number of gonadotropes, was significantly greater in females than in males throughout the year. In males that had a typical aquatic-phase morph and actively exhibited reproductive behavior, both area and number of gonadotropes, as well as profile estimates of pars distalis size, were the greatest of all monthly values. In females, gonadotrope cells increased in area and number from July-January during the terrestrial phase of the nonbreeding season. This increase was concurrent with the development of ovaries. This consecutive increase and development may explain the absence of a second oogenic phase during early spring.

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  • Intersexual differences and seasonal changes in gonadotropin-producing cells in the salamander Hynobius nigrescens

    M Hasumi, S Sasaki, O Hosoya, K Sato, H Haraguchi

    JOURNAL OF HERPETOLOGY   31 ( 1 )   45 - 51   1997年3月

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    記述言語:英語   出版者・発行元:SOC STUDY AMPHIBIANS REPTILES  

    Intersexual differences and annual cytological changes in presumptive gonadotropin-producing cells (gonadotropes) were examined immunohistochemically in mid-sagittal sections of the pituitary gland of adult Hynobius nigrescens by the use of a monoclonal antibody against bullfrog luteinizing hormone beta-subunit (LH beta). Total immunoreactive areas for LH beta and profiles of the pars distalis in the mid-sagittal plane of the gland were estimated with an image analyzer. Gonadotropes consistently were distributed in the pars distalis near the median eminence and the pars intermedia in both sexes throughout the year; an intersexual difference was found in their distribution pattern. Gonadotrope cells other than these consistent ones distributed in the peripheral region of the pars distalis frequently were observed in females, but not in males. The proportion of gonadotropes among all pars distalis cells, as well as the mean area and number of gonadotropes, was significantly greater in females than in males throughout the year. In males that had a typical aquatic-phase morph and actively exhibited reproductive behavior, both area and number of gonadotropes, as well as profile estimates of pars distalis size, were the greatest of all monthly values. In females, gonadotrope cells increased in area and number from July-January during the terrestrial phase of the nonbreeding season. This increase was concurrent with the development of ovaries. This consecutive increase and development may explain the absence of a second oogenic phase during early spring.

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  • Possible involvement of folliculo-stellate cells in the differentiation of muscle fibers during monolayer culture of pituitary cells

    O Hosoya, YG Watanabe

    ZOOLOGICAL SCIENCE   14 ( 1 )   141 - 145   1997年2月

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    記述言語:英語   出版者・発行元:ZOOLOGICAL SOC JAPAN  

    A major objective of the present study was to examine the possibility that non-granular folliculo-stellate (FS) cells in the rat anterior pituitary are involved in the myogenesis that occurs during pituitary cell culture. Enzymatically dissociated anterior pituitary cells were fractionated by use of the Percoll gradient method. The proportion of FS cells was 5.8% on average before cell fractionation. After employing the Percoll gradient procedure, FS cells were enriched to a ratio of 12.2%. Three of five cell fractions were separately cultured, and the incidence of striated muscle fibers was quantitatively investigated. There was a good correlation between the numbers of muscle fibers and the proportions of FS cells in the fractions obtained from the Percoll gradient. These results suggest that FS cells are the cells that transform into striated muscles in pituitary monolayer cultures.

    DOI: 10.2108/zsj.14.141

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  • Possible involvement of folliculo-stellate cells in the differentiation of muscle fibers during monolayer culture of pituitary cells

    Osamu Hosoya, Yuichi G. Watanabe

    Zoological Science   14 ( 1 )   141 - 145   1997年

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    記述言語:英語   出版者・発行元:Zoological Society of Japan  

    A major objective of the present study was to examine the possibility that non-granular folliculostellate (FS) cells in the rat anterior pituitary are involved in the myogenesis that occurs during pituitary cell culture. Enzymatically dissociated anterior pituitary cells were fractionated by use of the Percoll gradient method. The proportion of FS cells was 5.8% on average before cell fractionation. After employing the Percoll gradient procedure, FS cells were enriched to a ratio of 12.2%. Three of five cell fractions were separately cultured, and the incidence of striated muscle fibers was quantitatively investigated. There was a good correlation between the numbers of muscle fibers and the proportions of FS cells in the fractions obtained from the Percoll gradient. These results suggest that FS cells are the cells that transform into striated muscles in pituitary monolayer cultures.

    DOI: 10.2108/zsj.14.141

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▼全件表示

講演・口頭発表等

  • NK4 dye reduces the apoptosis of photoreceptor cells.

    ARVO (The Association for Research in Vision and Ophthalmology) 2014 Annual Meeting  2017年 

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  • NK4 dye reduces the apoptosis of photoreceptor cells.

    ARVO (The Association for Research in Vision and Ophthalmology) 2014 Annual Meeting  2017年 

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  • Apoptosis reduction by a photoelectric dye used for Okayama University-type retinal prosthesis (OURePTM).

    ARVO (The Association for Research in Vision and Ophthalmology) 2014 Annual Meeting  2016年 

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  • 神経細胞終末分化過程でのDNAトポイソメラーゼIIβによるグローバルなクロマチン構造変換を介した遺伝子発現制御機構.

    第33回染色体ワークショップ・第14回核ダイナミクス研究会  2016年 

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  • Apoptosis reduction by a photoelectric dye used for Okayama University-type retinal prosthesis (OURePTM).

    ARVO (The Association for Research in Vision and Ophthalmology) 2014 Annual Meeting  2016年 

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  • 神経細胞核の3次元DNA配置と遺伝子発現制御

    バイオイメージバイオインフォマティクスワークショップ2015  2015年 

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  • DNA topoisomerase IIβ mediates interactions between distal genomic sites within intergenic regions

    第38回日本分子生物学会年会, 第88回日本生化学会大会 合同大会  2015年 

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  • Global changes of chromatin structure during terminal differentiation of neurons are regulated by DNA topoisomerase IIβ complexed with hnRNPU/SAF-A/SP120

    第38回日本分子生物学会年会, 第88回日本生化学会大会 合同大会  2015年 

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  • Vision recovery and retinal apoptosis reduction in RCS rats with Okayama University-type retinal prothesis.

    ARVO (The Association for Research in Vision and Ophthalmology) 2014 Annual Meeting  2014年 

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  • RNA regulates nuclear dynamics and catalytic activity of topoisomerase IIbeta by binding to a novel domain of the enzyme

    Cell Symposia Regulatory RNAs  2014年 

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  • DNAトポイソメラーゼIIβによる核内構造変化と遺伝子発現制御

    第37回日本分子生物学会年会  2014年 

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  • RNA regulates nuclear dynamics and catalytic activity of topoisomerase IIbeta by binding to a novel domain of the enzyme

    Cell Symposia Regulatory RNAs  2014年 

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  • トポイソメラーゼIIβの酵素活性と核内動体はC末端ドメインへのRNAの結合によって制御されている

    第32回染色体ワークショップ・第13回核ダイナミクス研究会合  2014年 

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  • Vision recovery and retinal apoptosis reduction in RCS rats with Okayama University-type retinal prothesis.

    ARVO (The Association for Research in Vision and Ophthalmology) 2014 Annual Meeting  2014年 

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  • Regulatory roles of DNA topoisomerase IIβ in the developing cerebellar neurons.

    第4回国際小脳学会  2011年 

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  • 網膜視細胞の終末分化におけるDNAトポイソメラーゼIIβの役割

    第34回 日本分子生物学会年会  2011年 

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  • DNAトポイソメラーゼⅡβのRNAによる活性調節と核内局在変化

    第34回 日本分子生物学会年会  2011年 

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  • 網膜杆体視細胞の終末分化におけるDNAトポイソメラーゼIIβの関与

    第33回 日本分子生物学会年会 第83回 日本生化学会大会 合同大会  2010年 

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  • DNAトポイソメラーゼIIβの核内における移動メカニズム

    第33回 日本分子生物学会年会 第83回 日本生化学会大会 合同大会  2010年 

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  • 人工網膜からみた網膜神経細胞の機能

    第114回日本解剖学会総会・全国学術集会  2009年 

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  • 網膜リボンシナプスの情報伝達と網膜特異的Amphiphysin-Irの機能

    第114回日本解剖学会総会・全国学術集会  2009年 

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  • Internuclear dostribution of LEDGF/p75/SBP75 in HeLa cells

    2009年 

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  • LEDGF/p75/SBP75の超らせんDNA結合活性と核内局在

    第32回日本分子生物学年会年会  2009年 

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  • トポイソメラーゼIIβによる遺伝子活性化のメカニズム

    細胞核ダイナミクス 第五回班会議  2008年 

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  • DNAトポイソメラーゼIIβと核マトリックスタンパク質SP120/hnRNP U/SAF-Aの複合体形成

    第31回日本分子生物学年会年会 第81回日本生化学会大会 合同大会  2008年 

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  • 転写活性クロマチン領域へのHIV-1 DNA挿入を制御するLEDGF/p75/SBP75のクロマチン認識機構

    第31回日本分子生物学年会年会 第81回日本生化学会大会 合同大会  2008年 

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  • DNAトポイソメラーゼIIβの核質-核小体シャトリング機構

    第31回日本分子生物学年会年会 第81回日本生化学会大会 合同大会  2008年 

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  • Amphiphysin Ir participates in receptor-mediated endocytosis in ARPE-19

    Neuro2007  2007年 

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  • 神経細胞終末分化における遺伝子誘導とトポイソメラーゼIIβの標的

    特定領域研究「細胞核ダイナミクス」第4回班会議  2007年 

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  • Dynamic relocation and sumo-mediated degradation of DNA topoisomerase II beta under chromatin stress

    International symposium: Functional organization of the nucleus  2007年 

     詳細を見る

  • DNA topoisomerase II beta changes chromatin structure that is essential for transcriptional induction in the terminally differentiating neuron

    20th IUBMB International congress of biochemistry and molecular biology and 11th FAOBMB congress  2006年 

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  • 哺乳類II型トポイソメラーゼアイソフォームの動態と機能分担

    第23回染色体ワークショップ  2006年 

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  • AEPR-19細胞における網膜特異的amphiphysin Iスプライスバリアントの局在

    第29回 日本神経科学大会  2006年 

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  • 「細胞分化に伴う転写制御を可能にする染色体構造変換のメカニズム」

    第28回日本分子生物学会年会  2005年 

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  • Mechanism of the selective degradation of DNA topoisomerase II beta induced by a catalytic inhibitor ICRF-193

    第78回 日本生化学会大会  2005年 

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  • 核のストレス応答;DNAトポイソメラーゼIIβの誘導的分解

    第5回 細胞核ダイナミクス研究会  2005年 

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  • Molecular mechanism of endocytosis following tonic neurotransmitter release in the retinal ribbon synapses.

    International symposium on nano-biotechnology & 6th international conference on protein phosphatases  2004年 

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  • 網膜リボンシナプスにおけるamphiphysin Irの機能

    第26回日本神経科学大会  2004年 

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  • 神経細胞の分化過程における遺伝子の発現誘導とDNAトポイソメラーゼIIβによるクロマチン高次構造の変化

    第4回細胞核ダイナミクス研究会  2004年 

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  • Binding partners of the retina-specific amphiphysin I variants imply its role in the endocytic process unique to ribbon synapses.

    第77回日本生化学会大会  2004年 

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  • シナプス解析のための免疫組織化学の基礎

    第31回岡山脳研究セミナー  2004年 

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  • DNAトポイソメラーゼIIβ誘導分解におけるSUMO修飾の役割

    第27回日本分子生物学会年会  2004年 

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  • II型DNAトポイソメラーゼ複合体とクロマチン高次構造の変換

    第26回日本分子生物学会年会  2003年 

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  • 網膜リボンシナプスに局在する新規amphiphysin Iスプライスバリアント, amphiphysin Irとその結合タンパク質

    第25回 日本神経科学大会  2002年 

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  • 網膜で発現するシナプスタンパク質amphiphysin Irの局在

    第24回日本神経科学・第44回日本神経化学合同大会(Neuro2001)  2001年 

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  • 網膜リボンシナプスに特異的なamphiphysin Iスプライスバリアント

    第24回日本分子生物学会  2001年 

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▼全件表示

受賞

  • 2014年度日本臓器学会論文賞

    2014年  

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    受賞国:日本国

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  • TOMINAGA AWARD 2012

    2013年  

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    受賞国:日本国

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担当授業科目

  • 人体の構造:入門 (2021年度) 特別  - その他

  • 人体構造学 (2021年度) 集中  - その他

  • 神経構造学 (2021年度) 特別  - その他

  • 神経構造学実習 (2021年度) 特別  - その他

  • 脳神経機構学I(演習・実習) (2021年度) 特別  - その他

  • 脳神経機構学I(講義・演習) (2021年度) 特別  - その他

  • 脳神経機構学II(演習・実習) (2021年度) 特別  - その他

  • 脳神経機構学II(講義・演習) (2021年度) 特別  - その他

  • 人体の構造:入門 (2020年度) 特別  - その他

  • 人体構造学 (2020年度) 集中  - その他

  • 神経構造学 (2020年度) 特別  - その他

  • 神経構造学実習 (2020年度) 特別  - その他

  • 脳神経機構学I(演習・実習) (2020年度) 特別  - その他

  • 脳神経機構学I(講義・演習) (2020年度) 特別  - その他

  • 脳神経機構学II(演習・実習) (2020年度) 特別  - その他

  • 脳神経機構学II(講義・演習) (2020年度) 特別  - その他

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