研究者詳細 - 石川　一也

論文 - 石川　一也

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Structural and functional relationships between plasmodesmata and plant endoplasmic reticulum–plasma membrane contact sites consisting of three synaptotagmins 査読国際誌

Kazuya Ishikawa, Kentaro Tamura, Yoichiro Fukao, Tomoo Shimada

New Phytologist 226 ( 3 ) 798 - 808 2020年5月

担当区分：筆頭著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Wiley

Synaptotagmin 1 (SYT1) has been recognised as a tethering factor of plant endoplasmic reticulum (ER)-plasma membrane (PM) contact sites (EPCSs) and partially localises to around plasmodesmata (PD). However, other components of EPCSs associated with SYT1 and functional links between the EPCSs and PD have not been identified. We explored interactors of SYT1 by immunoprecipitation and mass analysis. The dynamics, morphology and spatial arrangement of the ER in Arabidopsis mutants lacking the EPCS components were investigated using confocal microscopy and electron microscopy. PD permeability of EPCS mutants was assessed using a virus movement protein and free green fluorescent protein (GFP) as indicators. We identified two additional components of the EPCSs, SYT5 and SYT7, that interact with SYT1. The mutants of the three SYTs were defective in the anchoring of the ER to the PM. The ER near the PD entrance appeared to be weakly squeezed in the triple mutant compared with the wild-type. The triple mutant suppressed cell-to-cell movement of the virus movement protein, but not GFP diffusion. We revealed major additional components of EPCS associated with SYT1 and suggested that the EPCSs arranged around the PD squeeze the ER to regulate active transport via PD.

DOI： 10.1111/nph.16391

PubMed

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その他リンク： https://onlinelibrary.wiley.com/doi/full-xml/10.1111/nph.16391

Subcellular localisation of an endoplasmic reticulum-plasma membrane tethering factor, SYNAPTOTAGMIN 1, is affected by fluorescent protein fusion 招待査読

Kazuya Ishikawa, Kentaro Tamura, Tomoo Shimada

Plant Signaling & Behavior 13 ( 12 ) e1547577 - e1547577 2018年12月

Synaptotagmin-Associated Endoplasmic Reticulum-Plasma Membrane Contact Sites Are Localized to Immobile ER Tubules 査読国際誌

Kazuya Ishikawa, Kentaro Tamura, Haruko Ueda, Yoko Ito, Akihiko Nakano, Ikuko Hara-Nishimura, Tomoo Shimada

Plant Physiology 178 ( 2 ) 641 - 653 2018年10月

担当区分：筆頭著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：American Society of Plant Biologists (ASPB)

The plant endoplasmic reticulum (ER), which is morphologically divided into tubules and sheets, seems to flow continuously as a whole, but locally, mobile and immobile regions exist. In eukaryotes, the ER physically and functionally interacts with the plasma membrane (PM) at domains called ER-PM contact sites (EPCSs). Extended synaptotagmin family proteins are concentrated in the cortical ER to form one type of EPCS; however, it is unclear whether the localization of extended synaptotagmin corresponds to the EPCS and where in the cortical ER the EPCSs are formed. Here, we analyzed the spatiotemporal localization of SYNAPTOTAGMIN1 (SYT1), a synaptotagmin in Arabidopsis (Arabidopsis thaliana), to investigate the precise distribution of SYT1-associated EPCSs in the cortical ER. Three-dimensional imaging using superresolution confocal live imaging microscopy demonstrated that SYT1 was specifically localized to the ER-PM boundary. Time-lapse imaging revealed that SYT1 was distributed to immobile ER tubules, but not to mobile tubules. Moreover, SYT1 was frequently localized to the edges of ER sheets that were transformed into immobile ER tubules over time. A lower intracellular calcium ion concentration resulted in an increased EPCS area and disrupted the ER network. Finally, SYT1 deficiency caused a reduction of the immobile tubules and enlargement of the ER meshes. Taken together, our findings show that SYT1-associated EPCS are distributed to immobile tubules and play an important role in the formation of the tubular ER network. This provides important insight into the relationship between the function and dynamics/morphology of the cortical ER.

DOI： 10.1104/pp.18.00498

PubMed

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Dual targeting of a virus movement protein to ER and plasma membrane subdomains is essential for plasmodesmata localization 査読

Kazuya Ishikawa, Masayoshi Hashimoto, Akira Yusa, Hiroaki Koinuma, Yugo Kitazawa, Osamu Netsu, Yasuyuki Yamaji, Shigetou Namba

PLOS Pathogens 13 ( 6 ) e1006463 - e1006463 2017年6月

EXA1, a GYF domain protein, is responsible for loss-of-susceptibility to plantago asiatica mosaic virus in Arabidopsis thaliana 査読

Masayoshi Hashimoto, Yutaro Neriya, Takuya Keima, Nozomu Iwabuchi, Hiroaki Koinuma, Yuka Hagiwara-Komoda, Kazuya Ishikawa, Misako Himeno, Kensaku Maejima, Yasuyuki Yamaji, Shigetou Namba

The Plant Journal 88 ( 1 ) 120 - 131 2016年10月

Passive virus movements with organelle dynamics 招待査読

Kazuya Ishikawa, Masayoshi Hashimoto, Shigetou Namba

Oncotarget 6 ( 31 ) 30437 - 30438 2015年10月

Rapid detection of fig mosaic virus using reverse transcription loop-mediated isothermal amplification 査読

Kazuya Ishikawa, Kensaku Maejima, Osamu Netsu, Misato Fukuoka, Takamichi Nijo, Masayoshi Hashimoto, Daisuke Takata, Yasuyuki Yamaji, Shigetou Namba

Journal of General Plant Pathology 81 ( 5 ) 382 - 389 2015年9月

Cell Death Triggered by a Putative Amphipathic Helix of Radish mosaic virus Helicase Protein Is Tightly Correlated With Host Membrane Modification 査読

Masayoshi Hashimoto, Ken Komatsu, Ryo Iwai, Takuya Keima, Kensaku Maejima, Takuya Shiraishi, Kazuya Ishikawa, Tetsuya Yoshida, Yugo Kitazawa, Yukari Okano, Yasuyuki Yamaji, Shigetou Namba

Molecular Plant-Microbe Interactions 28 ( 6 ) 675 - 688 2015年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Scientific Societies

Systemic necrosis is one of the most severe symptoms caused by plant RNA viruses. Recently, systemic necrosis has been suggested to have similar features to a defense response referred to as the hypersensitive response (HR), a form of programmed cell death. In virus-infected plant cells, host intracellular membrane structures are changed dramatically for more efficient viral replication. However, little is known about whether this replication-associated membrane modification is the cause of the symptoms. In this study, we identified an amino-terminal amphipathic helix of the helicase encoded by Radish mosaic virus (RaMV) (genus Comovirus) as an elicitor of cell death in RaMV-infected plants. Cell death caused by the amphipathic helix had features similar to HR, such as SGT1-dependence. Mutational analyses and inhibitor assays using cerulenin demonstrated that the amphipathic helix–induced cell death was tightly correlated with dramatic alterations in endoplasmic reticulum (ER) membrane structures. Furthermore, the cell death–inducing activity of the amphipathic helix was conserved in Cowpea mosaic virus (genus Comovirus) and Tobacco ringspot virus (genus Nepovirus), both of which are classified in the family Secoviridae. Together, these results indicate that ER membrane modification associated with viral intracellular replication may be recognized to prime defense responses against plant viruses.

DOI： 10.1094/mpmi-01-15-0004-r

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Nucleocapsid Protein from Fig Mosaic Virus Forms Cytoplasmic Agglomerates That Are Hauled by Endoplasmic Reticulum Streaming 査読

Kazuya Ishikawa, Chihiro Miura, Kensaku Maejima, Ken Komatsu, Masayoshi Hashimoto, Tatsuya Tomomitsu, Misato Fukuoka, Akira Yusa, Yasuyuki Yamaji, Shigetou Namba

Journal of Virology 89 ( 1 ) 480 - 491 2015年1月

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：American Society for Microbiology

<title>ABSTRACT</title>Although many studies have demonstrated intracellular movement of viral proteins or viral replication complexes, little is known about the mechanisms of their motility. In this study, we analyzed the localization and motility of the nucleocapsid protein (NP) of<italic>Fig mosaic virus</italic>(FMV), a negative-strand RNA virus belonging to the recently established genus<named-content content-type="genus-species">Emaravirus</named-content>. Electron microscopy of FMV-infected cells using immunogold labeling showed that NPs formed cytoplasmic agglomerates that were predominantly enveloped by the endoplasmic reticulum (ER) membrane, while nonenveloped NP agglomerates also localized along the ER. Likewise, transiently expressed NPs formed agglomerates, designated NP bodies (NBs), in close proximity to the ER, as was the case in FMV-infected cells. Subcellular fractionation and electron microscopic analyses of NP-expressing cells revealed that NBs localized in the cytoplasm. Furthermore, we found that NBs moved rapidly with the streaming of the ER in an actomyosin-dependent manner. Brefeldin A treatment at a high concentration to disturb the ER network configuration induced aberrant accumulation of NBs in the perinuclear region, indicating that the ER network configuration is related to NB localization. Dominant negative inhibition of the class XI myosins, XI-1, XI-2, and XI-K, affected both ER streaming and NB movement in a similar pattern. Taken together, these results showed that NBs localize in the cytoplasm but in close proximity to the ER membrane to form enveloped particles and that this causes passive movements of cytoplasmic NBs by ER streaming.

<bold>IMPORTANCE</bold>Intracellular trafficking is a primary and essential step for the cell-to-cell movement of viruses. To date, many studies have demonstrated the rapid intracellular movement of viral factors but have failed to provide evidence for the mechanism or biological significance of this motility. Here, we observed that agglomerates of nucleocapsid protein (NP) moved rapidly throughout the cell, and we performed live imaging and ultrastructural analysis to identify the mechanism of motility. We provide evidence that cytoplasmic protein agglomerates were passively dragged by actomyosin-mediated streaming of the endoplasmic reticulum (ER) in plant cells. In virus-infected cells, NP agglomerates were surrounded by the ER membranes, indicating that NP agglomerates form the basis of enveloped virus particles in close proximity to the ER. Our work provides a sophisticated model of macromolecular trafficking in plant cells and improves our understanding of the formation of enveloped particles of negative-strand RNA viruses.

DOI： 10.1128/jvi.02527-14

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Complete nucleotide sequence and genome structure of a Japanese isolate of hibiscus latent Fort Pierce virus, a unique tobamovirus that contains an internal poly(A) region in its 3′ end 査読

Tetsuya Yoshida, Yugo Kitazawa, Ken Komatsu, Yutaro Neriya, Kazuya Ishikawa, Naoko Fujita, Masayoshi Hashimoto, Kensaku Maejima, Yasuyuki Yamaji, Shigetou Namba

Archives of Virology 159 ( 11 ) 3161 - 3165 2014年11月

Recognition of floral homeotic MADS domain transcription factors by a phytoplasmal effector, phyllogen, induces phyllody 査読

Kensaku Maejima, Ryo Iwai, Misako Himeno, Ken Komatsu, Yugo Kitazawa, Naoko Fujita, Kazuya Ishikawa, Misato Fukuoka, Nami Minato, Yasuyuki Yamaji, Kenro Oshima, Shigetou Namba

The Plant Journal 78 ( 4 ) 541 - 554 2014年5月

Development of an on-site plum pox virus detection kit based on immunochromatography 査読

Kensaku Maejima, Misako Himeno, Osamu Netsu, Kazuya Ishikawa, Tetsuya Yoshida, Naoko Fujita, Masayoshi Hashimoto, Ken Komatsu, Yasuyuki Yamaji, Shigetou Namba

Journal of General Plant Pathology 80 ( 2 ) 176 - 183 2014年3月

Fig mosaic emaravirus p4 protein is involved in cell-to-cell movement 査読

Kazuya Ishikawa, Kensaku Maejima, Ken Komatsu, Osamu Netsu, Takuya Keima, Takuya Shiraishi, Yukari Okano, Masayoshi Hashimoto, Yasuyuki Yamaji, Shigetou Namba

Journal of General Virology 94 ( 3 ) 682 - 686 2013年3月

Identification and characterization of two novel genomic RNA segments of fig mosaic virus, RNA5 and RNA6 査読

Kazuya Ishikawa, Kensaku Maejima, Ken Komatsu, Yugo Kitazawa, Masayoshi Hashimoto, Daisuke Takata, Yasuyuki Yamaji, Shigetou Namba

Journal of General Virology 93 ( 7 ) 1612 - 1619 2012年7月

Infection of capilloviruses requires subgenomic RNAs whose transcription is controlled by promoter-like sequences conserved among flexiviruses 査読

Ken Komatsu, Hisae Hirata, Takako Fukagawa, Yasuyuki Yamaji, Yukari Okano, Kazuya Ishikawa, Tatsushi Adachi, Kensaku Maejima, Masayoshi Hashimoto, Shigetou Namba

Virus Research 167 ( 1 ) 8 - 15 2012年7月

First report of fig mosaic virus infecting common fig (Ficus carica) in Japan 査読

Kazuya Ishikawa, Kensaku Maejima, Susumu Nagashima, Nobuo Sawamura, Yusuke Takinami, Ken Komatsu, Masayoshi Hashimoto, Yasuyuki Yamaji, Jun Yamamoto, Shigetou Namba

Journal of General Plant Pathology 78 ( 2 ) 136 - 139 2012年1月

Identification of three MAPKKKs forming a linear signaling pathway leading to programmed cell death in Nicotiana benthamiana 査読

Masayoshi Hashimoto, Ken Komatsu, Kensaku Maejima, Yukari Okano, Takuya Shiraishi, Kazuya Ishikawa, Yusuke Takinami, Yasuyuki Yamaji, Shigetou Namba

BMC Plant Biology 12 ( 1 ) 103 - 103 2012年

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