2024/03/15 更新

写真a

イシカワ カズヤ
石川 一也
Ishikawa Kazuya
所属
医歯薬学域 助教
職名
助教
外部リンク

学位

  • 博士(農学) ( 2016年3月   東京大学 )

研究キーワード

  • 植物細胞 ライブイメージング 細菌

研究分野

  • 自然科学一般 / 地球生命科学  / 植物生理学 細胞生物学 微生物学

経歴

  • 岡山大学   学術研究院医歯薬学域(薬学系)   助教

    2022年4月 - 現在

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  • 宇都宮大学   バイオサイエンス教育研究センター   特任助教

    2019年2月 - 2022年3月

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  • 京都大学   大学院理学研究科 生物科学専攻   日本学術振興会特別研究員(PD)

    2016年4月 - 2019年1月

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  • 東京大学   大学院農学生命科学研究科   日本学術振興会特別研究員(DC1)

    2013年4月 - 2016年3月

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所属学協会

  • 日本細菌学会

    2023年2月 - 現在

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  • 日本植物生理学会

    2015年10月 - 現在

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論文

  • Bilirubin Distribution in Plants at the Subcellular and Tissue Levels 査読

    Kazuya Ishikawa, Yutaka Kodama

    Plant And Cell Physiology   2024年2月

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    担当区分:筆頭著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

    Abstract

    In heterotrophs, heme degradation produces bilirubin, a tetrapyrrole compound that has antioxidant activity. In plants, heme is degraded in plastids and is believed to be converted to phytochromobilin rather than bilirubin. Recently, we used the bilirubin-inducible fluorescent protein UnaG to reveal that plants produce bilirubin via a non-enzymatic reaction with NADPH. In the present study, we used an UnaG-based live imaging system to visualize bilirubin accumulation in Arabidopsis thaliana and Nicotiana benthamiana at the organelle and tissue levels. In chloroplasts, bilirubin preferentially accumulated in the stroma, and the stromal bilirubin level increased upon dark treatment. Investigation of intracellular bilirubin distribution in leaves and roots showed that it accumulated mostly in plastids, with low levels detected in the cytosol and other organelles, such as peroxisomes, mitochondria and the endoplasmic reticulum. A treatment that increased bilirubin production in chloroplasts decreased the bilirubin level in peroxisomes, implying that a bilirubin precursor is transported between the two organelles. At the cell and tissue levels, bilirubin showed substantial accumulation in the root elongation region but little or none in the root cap and guard cells. Intermediate bilirubin accumulation was observed in other shoot and root tissues, with lower levels in shoot tissues. Our data revealed the distribution of bilirubin in plants, which has implications for the transport and physiological function of tetrapyrroles.

    DOI: 10.1093/pcp/pcae017

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  • Knockout of adenylosuccinate synthase purA increases susceptibility to colistin in Escherichia coli

    Tomonori Kano, Kazuya Ishikawa, Kazuyuki Furuta, Chikara Kaito

    FEMS Microbiology Letters   371   fnae007   2024年2月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Oxford University Press (OUP)  

    Abstract

    Colistin is a cationic cyclic antimicrobial peptide used as a last resort against multidrug-resistant gram-negative bacteria. To understand the factors involved in colistin susceptibility, we screened colistin-sensitive mutants from an E. coli gene-knockout library (Keio collection). The knockout of purA, whose product catalyzes the synthesis of adenylosuccinate from IMP in the de novo purine synthesis pathway, resulted in increased sensitivity to colistin. Adenylosuccinate is subsequently converted to AMP, which is phosphorylated to produce ADP, a substrate for ATP synthesis. The amount of ATP was lower in the purA-knockout mutant than that in the wild-type strain. ATP synthesis is coupled with proton transfer, and it contributes to the membrane potential. Using the membrane potential probe, 3,3′-diethyloxacarbocyanine iodide [DiOC2(3)], we found that the membrane was hyperpolarized in the purA-knockout mutant compared to that in the wild-type strain. Treatment with the proton uncoupler, carbonyl cyanide m-chlorophenyl hydrazone (CCCP), abolished the hyperpolarization and colistin sensitivity in the mutant. The purA-knockout mutant exhibited increased sensitivity to aminoglycosides, kanamycin, and gentamicin; their uptake requires a membrane potential. Therefore, the knockout of purA, an adenylosuccinate synthase, decreases ATP synthesis concurrently with membrane hyperpolarization, resulting in increased sensitivity to colistin.

    DOI: 10.1093/femsle/fnae007

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  • Short‐chain fatty acids stimulate dendrite elongation in dendritic cells by inhibiting histone deacetylase 査読

    Takuho Inamoto, Kazuyuki Furuta, Cheng Han, Mio Uneme, Tomonori Kano, Kazuya Ishikawa, Chikara Kaito

    The FEBS Journal   290 ( 24 )   5794 - 5810   2023年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    Dendritic cells activate immune responses by presenting pathogen‐derived molecules. The dendrites of dendritic cells contribute to the incorporation of foreign antigens or presenting antigens to T cells. Short‐chain fatty acids (SCFAs), such as acetic, propionic, butyric and valeric acids, have many effects on immune responses by activating specific receptors or inhibiting a histone deacetylase (HDAC), although their effect on dendrite formation in dendritic cells is unknown. In the present study, we aimed to investigate the effect of SCFAs on dendrite elongation using a dendritic cell line (DC2.4 cells) and mouse bone marrow‐derived dendritic cells. We found that SCFAs induced dendrite elongation. The elongation was reduced by inhibitors of Src family kinase (SFK), phosphatidylinositol‐3 kinase (PI3K), Rho family GTPases (Cdc42, Rac1) or actin polymerization, indicating that SCFAs promote dendrite elongation by activating actin polymerization via the SFK/PI3K/Rho family GTPase signaling pathway. We showed that agonists for SCFA receptors GPR43 and GPR109a did not promote dendrite elongation. By contrast, HDAC inhibitors, including trichostatin A, promoted dendrite elongation in DC2.4 cells, and the promoting activity of trichostatin A was decreased by inhibiting the SFK/PI3K/Rho family GTPase signaling pathway or actin polymerization. Furthermore, DC2.4 cells treated with valeric acid showed enhanced uptake of soluble proteins, insoluble beads and Staphylococcus aureus. We also found that treatment with valeric acid enhanced major histocompatibility complex class II‐mediated antigen presentation in bone marrow‐derived dendritic cells. These results suggest that SCFAs promote dendrite elongation by inhibiting HDAC, stimulating the SFK/PI3K/Rho family pathway and activating actin polymerization, resulting in increased antigen uptake and presentation in dendritic cells.

    DOI: 10.1111/febs.16945

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  • Bilirubin is produced nonenzymatically in plants to maintain chloroplast redox status 査読 国際誌

    Kazuya Ishikawa, Xiaonan Xie, Yasuhide Osaki, Atsushi Miyawaki, Keiji Numata, Yutaka Kodama

    Science Advances   9 ( 23 )   eadh478   2023年6月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Bilirubin, a potent antioxidant, is a product of heme catabolism in heterotrophs. Heterotrophs mitigate oxidative stress resulting from free heme by catabolism into bilirubin via biliverdin. Although plants also convert heme to biliverdin, they are generally thought to be incapable of producing bilirubin because they lack biliverdin reductase, the enzyme responsible for bilirubin biosynthesis in heterotrophs. Here, we demonstrate that bilirubin is produced in plant chloroplasts. Live-cell imaging using the bilirubin-dependent fluorescent protein UnaG revealed that bilirubin accumulated in chloroplasts. In vitro, bilirubin was produced nonenzymatically through a reaction between biliverdin and reduced form of nicotinamide adenine dinucleotide phosphate at concentrations comparable to those in chloroplasts. In addition, increased bilirubin production led to lower reactive oxygen species levels in chloroplasts. Our data refute the generally accepted pathway of heme degradation in plants and suggest that bilirubin contributes to the maintenance of redox status in chloroplasts.

    DOI: 10.1126/sciadv.adh4787

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  • Binding of Tau-derived peptide-fused GFP to plant microtubules in Arabidopsis thaliana 査読

    Hiroshi Inaba, Kazusato Oikawa, Kazuya Ishikawa, Yutaka Kodama, Kazunori Matsuura, Keiji Numata

    PLOS ONE   18 ( 6 )   e0286421   2023年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Public Library of Science (PLoS)  

    Studies on how exogenous molecules modulate properties of plant microtubules, such as their stability, structure, and dynamics, are important for understanding and modulating microtubule functions in plants. We have developed a Tau-derived peptide (TP) that binds to microtubules and modulates their properties by binding of TP-conjugated molecules in vitro. However, there was no investigation of TPs on microtubules in planta. Here, we generated transgenic Arabidopsis thaliana plants stably expressing TP-fused superfolder GFP (sfGFP-TP) and explored the binding properties and effects of sfGFP-TP on plant microtubules. Our results indicate that the expressed sfGFP-TP binds to the plant microtubules without inhibiting plant growth. A transgenic line strongly expressing sfGFP-TP produced thick fibrous structures that were stable under conditions where microtubules normally depolymerize. This study generates a new tool for analyzing and modulating plant microtubules.

    DOI: 10.1371/journal.pone.0286421

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  • Knockout of ribosomal protein RpmJ leads to zinc resistance in Escherichia coli 査読

    Riko Shirakawa, Kazuya Ishikawa, Kazuyuki Furuta, Chikara Kaito

    PLOS ONE   18 ( 3 )   e0277162   2023年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Public Library of Science (PLoS)  

    Zinc is an essential metal for cells, but excess amounts are toxic. Other than by regulating the intracellular zinc concentration by zinc uptake or efflux, the mechanisms underlying bacterial resistance to excess zinc are unknown. In the present study, we searched for zinc-resistant mutant strains from the Keio collection, a gene knockout library of Escherichia coli, a model gram-negative bacteria. We found that knockout mutant of RpmJ (L36), a 50S ribosomal protein, exhibited zinc resistance. The rpmJ mutant was sensitive to protein synthesis inhibitors and had altered translation fidelity, indicating ribosomal dysfunction. In the rpmJ mutant, the intracellular zinc concentration was decreased under excess zinc conditions. Knockout of ZntA, a zinc efflux pump, abolished the zinc-resistant phenotype of the rpmJ mutant. RNA sequence analysis revealed that the rpmJ mutant exhibited altered gene expression of diverse functional categories, including translation, energy metabolism, and stress response. These findings suggest that knocking out RpmJ alters gene expression patterns and causes zinc resistance by lowering the intracellular zinc concentration. Knockouts of other ribosomal proteins, including RplA, RpmE, RpmI, and RpsT, also led to a zinc-resistant phenotype, suggesting that deletion of ribosomal proteins is closely related to zinc resistance.

    DOI: 10.1371/journal.pone.0277162

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  • Using the organelle glue technique to engineer the plant cell metabolome 査読

    Kazuya Ishikawa, Makoto Kobayashi, Miyako Kusano, Keiji Numata, Yutaka Kodama

    Plant Cell Reports   42 ( 3 )   599 - 607   2023年1月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    DOI: 10.1007/s00299-023-02982-2

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    その他リンク: https://link.springer.com/article/10.1007/s00299-023-02982-2/fulltext.html

  • Knockout of ykcB, a Putative Glycosyltransferase, Leads to Reduced Susceptibility to Vancomycin in Bacillus subtilis 査読

    Kazuya Ishikawa, Riko Shirakawa, Daiki Takano, Tomoki Kosaki, Kazuyuki Furuta, Chikara Kaito

    Journal of Bacteriology   2022年11月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    Although vancomycin is effective against Gram-positive bacteria, vancomycin-resistant bacteria are a major public health concern. While the vancomycin-resistance mechanisms of clinically important bacteria such as Staphylococcus aureus , Enterococcus faecium , and Streptococcus pneumoniae are well studied, they remain unclear in other Gram-positive bacteria.

    DOI: 10.1128/jb.00387-22

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  • Live‐cell imaging of the chloroplast outer envelope membrane using fluorescent dyes 査読 国際誌

    Shintaro Ichikawa, Kazuya Ishikawa, Hitoshi Miyakawa, Yutaka Kodama

    Plant Direct   6 ( 11 )   e462   2022年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    UNLABELLED: Chloroplasts are organelles composed of sub-organellar compartments-stroma, thylakoids, and starch granules-and are surrounded by outer and inner envelope membranes (OEM and IEM, respectively). The chloroplast OEM and IEM play key roles not only as a barrier separating the chloroplast components from the cytosol but also in the interchange of numerous metabolites and proteins between the chloroplast interior and the cytosol. Fluorescent protein markers for the chloroplast OEM have been widely used to visualize the outermost border of chloroplasts. However, the use of marker proteins requires an established cellular genetic transformation method, which limits the plant species in which marker proteins can be used. Moreover, the high accumulation of OEM marker proteins often elicits abnormal morphological phenotypes of the OEM. Because the OEM can currently only be visualized using exogenous marker proteins, the behaviors of the chloroplast and/or its OEM remain unknown in wild-type cells of various plant species. Here, we visualized the OEM using live-cell staining with the fluorescent dyes rhodamine B and Nile red in several plant species, including crops. We propose rhodamine B and Nile red as new tools for visualizing the chloroplast OEM in living plant cells that do not require genetic transformation. SIGNIFICANCE STATEMENT: We established a live-cell imaging method to visualize the chloroplast outer envelope membrane by staining living cells with fluorescent dyes. This method does not require genetic transformation and allows the observation of the chloroplast outer envelope membrane in various plant species.

    DOI: 10.1002/pld3.462

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    その他リンク: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/pld3.462

  • Organellar Glue: A Molecular Tool to Artificially Control Chloroplast–Chloroplast Interactions 査読 国際誌

    Shintaro Ichikawa, Shota Kato, Yuta Fujii, Kazuya Ishikawa, Keiji Numata, Yutaka Kodama

    ACS Synthetic Biology   11 ( 10 )   3190 - 3197   2022年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:American Chemical Society (ACS)  

    Organelles can physically interact to facilitate various cellular processes such as metabolite exchange. Artificially regulating these interactions represents a promising approach for synthetic biology. Here, we artificially controlled chloroplast-chloroplast interactions in living plant cells with our organelle glue (ORGL) technique, which is based on reconstitution of a split fluorescent protein. We simultaneously targeted N-terminal and C-terminal fragments of a fluorescent protein to the chloroplast outer envelope membrane or cytosol, respectively, which induced chloroplast-chloroplast interactions. The cytosolic C-terminal fragment likely functions as a bridge between two N-terminal fragments, thereby bringing the chloroplasts in close proximity to interact. We modulated the frequency of chloroplast-chloroplast interactions by altering the ratio of N- and C-terminal fragments. We conclude that the ORGL technique can successfully control chloroplast-chloroplast interactions in plants, providing a proof of concept for the artificial regulation of organelle interactions in living cells.

    DOI: 10.1021/acssynbio.2c00367

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  • The endoplasmic reticulum membrane–bending protein RETICULON facilitates chloroplast relocation movement in Marchantia polymorpha 査読 国際誌

    Kazuya Ishikawa, Ryota Konno, Satoyuki Hirano, Yuta Fujii, Masayuki Fujiwara, Yoichiro Fukao, Yutaka Kodama

    Plant Journal   111 ( 1 )   205 - 216   2022年7月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    Plant cells alter the intracellular positions of chloroplasts to ensure efficient photosynthesis, a process controlled by the blue light receptor phototropin. Chloroplasts migrate toward weak light (accumulation response) and move away from excess light (avoidance response). Chloroplasts are encircled by the endoplasmic reticulum (ER), which forms a complex network throughout the cytoplasm. To ensure rapid chloroplast relocation, the ER must alter its structure in conjunction with chloroplast relocation movement, but little is known about the underlying mechanism. Here, we searched for interactors of phototropin in the liverwort Marchantia polymorpha and identified a RETICULON (RTN) family protein; RTN proteins play central roles in ER tubule formation and ER network maintenance by stabilizing the curvature of ER membranes in eukaryotic cells. Marchantia polymorpha RTN1 (MpRTN1) is localized to ER tubules and the rims of ER sheets, which is consistent with the localization of RTNs in other plants and heterotrophs. The Mprtn1 mutant showed an increased ER tubule diameter, pointing to a role for MpRTN1 in ER membrane constriction. Furthermore, Mprtn1 showed a delayed chloroplast avoidance response but a normal chloroplast accumulation response. The live cell imaging of ER dynamics revealed that ER restructuring was impaired in Mprtn1 during the chloroplast avoidance response. These results suggest that during the chloroplast avoidance response, MpRTN1 restructures the ER network and facilitates chloroplast movement via an interaction with phototropin. Our findings provide evidence that plant cells respond to fluctuating environmental conditions by controlling the movements of multiple organelles in a synchronized manner.

    DOI: 10.1111/tpj.15787

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    その他リンク: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/tpj.15787

  • Arabidopsis group C Raf-like protein kinases negatively regulate abscisic acid signaling and are direct substrates of SnRK2 査読 国際誌

    Yoshiaki Kamiyama, Misaki Hirotani, Shinnosuke Ishikawa, Fuko Minegishi, Sotaro Katagiri, Conner J. Rogan, Fuminori Takahashi, Mika Nomoto, Kazuya Ishikawa, Yutaka Kodama, Yasuomi Tada, Daisuke Takezawa, Jeffrey C. Anderson, Scott C. Peck, Kazuo Shinozaki, Taishi Umezawa

    Proceedings of the National Academy of Sciences   118 ( 30 )   e2100073118 - e2100073118   2021年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Proceedings of the National Academy of Sciences  

    The phytohormone abscisic acid (ABA) plays a major role in abiotic stress responses in plants, and subclass III SNF1-related protein kinase 2 (SnRK2) kinases mediate ABA signaling. In this study, we identified Raf36, a group C Raf-like protein kinase in <italic>Arabidopsis</italic>, as a protein that interacts with multiple SnRK2s. A series of reverse genetic and biochemical analyses revealed that 1) Raf36 negatively regulates ABA responses during postgermination growth, 2) the N terminus of Raf36 is directly phosphorylated by SnRK2s, and 3) Raf36 degradation is enhanced in response to ABA. In addition, Raf22, another C-type Raf-like kinase, functions partially redundantly with Raf36 to regulate ABA responses. A comparative phosphoproteomic analysis of ABA-induced responses of wild-type and <italic>raf22raf36-1</italic> plants identified proteins that are phosphorylated downstream of Raf36 and Raf22 in planta. Together, these results support a model in which Raf36/Raf22 function mainly under optimal conditions to suppress ABA responses, whereas in response to ABA, the SnRK2 module promotes Raf36 degradation as a means of alleviating Raf36-dependent inhibition and allowing for heightened ABA signaling to occur.

    DOI: 10.1073/pnas.2100073118

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    その他リンク: https://syndication.highwire.org/content/doi/10.1073/pnas.2100073118

  • Mitochondrial movement during its association with chloroplasts in Arabidopsis thaliana 査読 国際誌

    Kazusato Oikawa, Takuto, Imai, Chonprakun Thagun, Kiminori Toyooka, Takeshi Yoshizumi, Kazuya Ishikawa, Yutaka Kodama, Keiji Numata

    Communications Biology   4 ( 1 )   1 - 13   2021年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Plant mitochondria move dynamically inside cells and this movement is classified into two types: directional movement, in which mitochondria travel long distances, and wiggling, in which mitochondria travel short distances. However, the underlying mechanisms and roles of both types of mitochondrial movement, especially wiggling, remain to be determined. Here, we used confocal laser-scanning microscopy to quantitatively characterize mitochondrial movement (rate and trajectory) in Arabidopsis thaliana mesophyll cells. Directional movement leading to long-distance migration occurred at high speed with a low angle-change rate, whereas wiggling leading to short-distance migration occurred at low speed with a high angle-change rate. The mean square displacement (MSD) analysis could separate these two movements. Directional movement was dependent on filamentous actin (F-actin), whereas mitochondrial wiggling was not, but slightly influenced by F-actin. In mesophyll cells, mitochondria could migrate by wiggling, and most of these mitochondria associated with chloroplasts. Thus, mitochondria migrate via F-actin-independent wiggling under the influence of F-actin during their association with chloroplasts in Arabidopsis.

    DOI: 10.1038/s42003-021-01833-8

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  • Apple latent spherical virus (ALSV)-induced gene silencing in a medicinal plant, Lithospermum erythrorhizon 査読 国際誌

    Yuki Izuishi, Natsumi Isaka, Hao Li, Kohei Nakanishi, Joji Kageyama, Kazuya Ishikawa, Tomoo Shimada, Chikara Masuta, Nobuyuki Yoshikawa, Hiroaki Kusano, Kazufumi Yazaki

    Scientific Reports   10 ( 1 )   13555 - 13555   2020年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    Lithospermum erythrorhizon is a medicinal plant that produces shikonin, a red lipophilic naphthoquinone derivative that accumulates exclusively in roots. The biosynthetic steps required to complete the naphthalene ring of shikonin and its mechanism of secretion remain unclear. Multiple omics studies identified several candidate genes involved in shikonin production. The functions of these genes can be evaluated using virus-induced gene silencing (VIGS) systems, which have been shown advantageous in introducing iRNA genes into non-model plants. This study describes the development of a VIGS system using an apple latent spherical virus (ALSV) vector and a target gene, phytoene desaturase (LePDS1). Virus particles packaged in Nicotiana benthamiana were inoculated into L. erythrorhizon seedlings, yielding new leaves with albino phenotype but without disease symptoms. The levels of LePDS1 mRNAs were significantly lower in the albino plants than in mock control or escape plants. Virus-derived mRNA was detected in infected plants but not in escape and mock plants. Quantitative PCR and deep sequencing analysis indicated that transcription of another hypothetical PDS gene (LePDS2) also decreased in the defective leaves. Virus infection, however, had no effect on shikonin production. These results suggest that virus-based genetic transformation and the VIGS system silence target genes in soil-grown L. erythrorhizon.

    DOI: 10.1038/s41598-020-70469-1

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    その他リンク: http://www.nature.com/articles/s41598-020-70469-1

  • Structural and functional relationships between plasmodesmata and plant endoplasmic reticulum–plasma membrane contact sites consisting of three synaptotagmins 査読 国際誌

    Kazuya Ishikawa, Kentaro Tamura, Yoichiro Fukao, Tomoo Shimada

    New Phytologist   226 ( 3 )   798 - 808   2020年5月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    Synaptotagmin 1 (SYT1) has been recognised as a tethering factor of plant endoplasmic reticulum (ER)-plasma membrane (PM) contact sites (EPCSs) and partially localises to around plasmodesmata (PD). However, other components of EPCSs associated with SYT1 and functional links between the EPCSs and PD have not been identified. We explored interactors of SYT1 by immunoprecipitation and mass analysis. The dynamics, morphology and spatial arrangement of the ER in Arabidopsis mutants lacking the EPCS components were investigated using confocal microscopy and electron microscopy. PD permeability of EPCS mutants was assessed using a virus movement protein and free green fluorescent protein (GFP) as indicators. We identified two additional components of the EPCSs, SYT5 and SYT7, that interact with SYT1. The mutants of the three SYTs were defective in the anchoring of the ER to the PM. The ER near the PD entrance appeared to be weakly squeezed in the triple mutant compared with the wild-type. The triple mutant suppressed cell-to-cell movement of the virus movement protein, but not GFP diffusion. We revealed major additional components of EPCS associated with SYT1 and suggested that the EPCSs arranged around the PD squeeze the ER to regulate active transport via PD.

    DOI: 10.1111/nph.16391

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    その他リンク: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/nph.16391

  • Subcellular localisation of an endoplasmic reticulum-plasma membrane tethering factor, SYNAPTOTAGMIN 1, is affected by fluorescent protein fusion 招待 査読

    Kazuya Ishikawa, Kentaro Tamura, Tomoo Shimada

    Plant Signaling & Behavior   13 ( 12 )   e1547577 - e1547577   2018年12月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Informa UK Limited  

    DOI: 10.1080/15592324.2018.1547577

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  • Synaptotagmin-Associated Endoplasmic Reticulum-Plasma Membrane Contact Sites Are Localized to Immobile ER Tubules 査読 国際誌

    Kazuya Ishikawa, Kentaro Tamura, Haruko Ueda, Yoko Ito, Akihiko Nakano, Ikuko Hara-Nishimura, Tomoo Shimada

    Plant Physiology   178 ( 2 )   641 - 653   2018年10月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society of Plant Biologists (ASPB)  

    The plant endoplasmic reticulum (ER), which is morphologically divided into tubules and sheets, seems to flow continuously as a whole, but locally, mobile and immobile regions exist. In eukaryotes, the ER physically and functionally interacts with the plasma membrane (PM) at domains called ER-PM contact sites (EPCSs). Extended synaptotagmin family proteins are concentrated in the cortical ER to form one type of EPCS; however, it is unclear whether the localization of extended synaptotagmin corresponds to the EPCS and where in the cortical ER the EPCSs are formed. Here, we analyzed the spatiotemporal localization of SYNAPTOTAGMIN1 (SYT1), a synaptotagmin in Arabidopsis (Arabidopsis thaliana), to investigate the precise distribution of SYT1-associated EPCSs in the cortical ER. Three-dimensional imaging using superresolution confocal live imaging microscopy demonstrated that SYT1 was specifically localized to the ER-PM boundary. Time-lapse imaging revealed that SYT1 was distributed to immobile ER tubules, but not to mobile tubules. Moreover, SYT1 was frequently localized to the edges of ER sheets that were transformed into immobile ER tubules over time. A lower intracellular calcium ion concentration resulted in an increased EPCS area and disrupted the ER network. Finally, SYT1 deficiency caused a reduction of the immobile tubules and enlargement of the ER meshes. Taken together, our findings show that SYT1-associated EPCS are distributed to immobile tubules and play an important role in the formation of the tubular ER network. This provides important insight into the relationship between the function and dynamics/morphology of the cortical ER.

    DOI: 10.1104/pp.18.00498

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  • Dual targeting of a virus movement protein to ER and plasma membrane subdomains is essential for plasmodesmata localization 査読

    Kazuya Ishikawa, Masayoshi Hashimoto, Akira Yusa, Hiroaki Koinuma, Yugo Kitazawa, Osamu Netsu, Yasuyuki Yamaji, Shigetou Namba

    PLOS Pathogens   13 ( 6 )   e1006463 - e1006463   2017年6月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Public Library of Science (PLoS)  

    Plant virus movement proteins (MPs) localize to plasmodesmata (PD) to facilitate virus cell-to-cell movement. Numerous studies have suggested that MPs use a pathway either through the ER or through the plasma membrane (PM). Furthermore, recent studies reported that ER-PM contact sites and PM microdomains, which are subdomains found in the ER and PM, are involved in virus cell-to-cell movement. However, functional relationship of these subdomains in MP traffic to PD has not been described previously. We demonstrate here the intracellular trafficking of fig mosaic virus MP (MPFMV) using live cell imaging, focusing on its ERdirecting signal peptide (SPFMV). Transiently expressed MPFMV was distributed predominantly in PD and patchy microdomains of the PM. Investigation of ER translocation efficiency revealed that SPFMV has quite low efficiency compared with SPs of well-characterized plant proteins, calreticulin and CLAVATA3. An MPFMV mutant lacking SPFMV localized exclusively to the PM microdomains, whereas SP chimeras, in which the SP of MPFMV was replaced by an SP of calreticulin or CLAVATA3, localized exclusively to the nodes of the ER, which was labeled with Arabidopsis synaptotagmin 1, a major component of ER-PM contact sites. From these results, we speculated that the low translocation efficiency of SPFMV contributes to the generation of ER-translocated and the microdomain-localized populations, both of which are necessary for PD localization. Consistent with this hypothesis, SP-deficient MPFMV became localized to PD when co-expressed with an SP chimera. Here we propose a new model for the intracellular trafficking of a viral MP. A substantial portion of MPFMV that fails to be translocated is transferred to the microdomains, whereas the remainder of MPFMV that is successfully translocated into the ER subsequently localizes to ER-PM contact sites and plays an important role in the entry of the microdomain-localized MPFMV into PD.

    DOI: 10.1371/journal.ppat.1006463

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  • EXA1, a GYF domain protein, is responsible for loss-of-susceptibility to plantago asiatica mosaic virus in Arabidopsis thaliana 査読

    Masayoshi Hashimoto, Yutaro Neriya, Takuya Keima, Nozomu Iwabuchi, Hiroaki Koinuma, Yuka Hagiwara-Komoda, Kazuya Ishikawa, Misako Himeno, Kensaku Maejima, Yasuyuki Yamaji, Shigetou Namba

    The Plant Journal   88 ( 1 )   120 - 131   2016年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/tpj.13265

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  • Passive virus movements with organelle dynamics 招待 査読

    Kazuya Ishikawa, Masayoshi Hashimoto, Shigetou Namba

    Oncotarget   6 ( 31 )   30437 - 30438   2015年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Impact Journals, LLC  

    DOI: 10.18632/oncotarget.5897

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  • Rapid detection of fig mosaic virus using reverse transcription loop-mediated isothermal amplification 査読

    Kazuya Ishikawa, Kensaku Maejima, Osamu Netsu, Misato Fukuoka, Takamichi Nijo, Masayoshi Hashimoto, Daisuke Takata, Yasuyuki Yamaji, Shigetou Namba

    Journal of General Plant Pathology   81 ( 5 )   382 - 389   2015年9月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    Fig mosaic virus (FMV), a negative-strand RNA virus, is a causal agent of fig mosaic disease, which occurs in almost all countries where figs are grown and severely affects worldwide fig production. Reverse transcription-polymerase chain reaction (RT-PCR) using FMV-specific primers has typically been used to detect FMV, but faster and easier detection methods for FMV are required because RT-PCR requires multiple steps and special equipment. In this study, we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method to detect FMV. We designed LAMP primer sets based on sequence conservation among FMV isolates. The RT-LAMP reaction using a primer set targeting RNA3 in total RNA extracted from FMV-infected fig leaves resulted in rapid detection within 15 min. In addition, we established a fast and simple method of direct sampling from fig leaves using a wooden toothpick. The RT-LAMP reaction specificity and reactivity when using the direct sampling method were almost comparable to those obtained using isolated total RNA. Moreover, geographically and phylogenetically distinct FMV isolates were detectable using the FMV RT-LAMP assay. The assay presented here provides a practical method to detect FMV.

    DOI: 10.1007/s10327-015-0603-1

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    その他リンク: http://link.springer.com/article/10.1007/s10327-015-0603-1/fulltext.html

  • Cell Death Triggered by a Putative Amphipathic Helix of Radish mosaic virus Helicase Protein Is Tightly Correlated With Host Membrane Modification 査読

    Masayoshi Hashimoto, Ken Komatsu, Ryo Iwai, Takuya Keima, Kensaku Maejima, Takuya Shiraishi, Kazuya Ishikawa, Tetsuya Yoshida, Yugo Kitazawa, Yukari Okano, Yasuyuki Yamaji, Shigetou Namba

    Molecular Plant-Microbe Interactions   28 ( 6 )   675 - 688   2015年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Scientific Societies  

    Systemic necrosis is one of the most severe symptoms caused by plant RNA viruses. Recently, systemic necrosis has been suggested to have similar features to a defense response referred to as the hypersensitive response (HR), a form of programmed cell death. In virus-infected plant cells, host intracellular membrane structures are changed dramatically for more efficient viral replication. However, little is known about whether this replication-associated membrane modification is the cause of the symptoms. In this study, we identified an amino-terminal amphipathic helix of the helicase encoded by Radish mosaic virus (RaMV) (genus Comovirus) as an elicitor of cell death in RaMV-infected plants. Cell death caused by the amphipathic helix had features similar to HR, such as SGT1-dependence. Mutational analyses and inhibitor assays using cerulenin demonstrated that the amphipathic helix–induced cell death was tightly correlated with dramatic alterations in endoplasmic reticulum (ER) membrane structures. Furthermore, the cell death–inducing activity of the amphipathic helix was conserved in Cowpea mosaic virus (genus Comovirus) and Tobacco ringspot virus (genus Nepovirus), both of which are classified in the family Secoviridae. Together, these results indicate that ER membrane modification associated with viral intracellular replication may be recognized to prime defense responses against plant viruses.

    DOI: 10.1094/mpmi-01-15-0004-r

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  • Nucleocapsid Protein from Fig Mosaic Virus Forms Cytoplasmic Agglomerates That Are Hauled by Endoplasmic Reticulum Streaming 査読

    Kazuya Ishikawa, Chihiro Miura, Kensaku Maejima, Ken Komatsu, Masayoshi Hashimoto, Tatsuya Tomomitsu, Misato Fukuoka, Akira Yusa, Yasuyuki Yamaji, Shigetou Namba

    Journal of Virology   89 ( 1 )   480 - 491   2015年1月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society for Microbiology  

    <title>ABSTRACT</title>Although many studies have demonstrated intracellular movement of viral proteins or viral replication complexes, little is known about the mechanisms of their motility. In this study, we analyzed the localization and motility of the nucleocapsid protein (NP) of<italic>Fig mosaic virus</italic>(FMV), a negative-strand RNA virus belonging to the recently established genus<named-content content-type="genus-species">Emaravirus</named-content>. Electron microscopy of FMV-infected cells using immunogold labeling showed that NPs formed cytoplasmic agglomerates that were predominantly enveloped by the endoplasmic reticulum (ER) membrane, while nonenveloped NP agglomerates also localized along the ER. Likewise, transiently expressed NPs formed agglomerates, designated NP bodies (NBs), in close proximity to the ER, as was the case in FMV-infected cells. Subcellular fractionation and electron microscopic analyses of NP-expressing cells revealed that NBs localized in the cytoplasm. Furthermore, we found that NBs moved rapidly with the streaming of the ER in an actomyosin-dependent manner. Brefeldin A treatment at a high concentration to disturb the ER network configuration induced aberrant accumulation of NBs in the perinuclear region, indicating that the ER network configuration is related to NB localization. Dominant negative inhibition of the class XI myosins, XI-1, XI-2, and XI-K, affected both ER streaming and NB movement in a similar pattern. Taken together, these results showed that NBs localize in the cytoplasm but in close proximity to the ER membrane to form enveloped particles and that this causes passive movements of cytoplasmic NBs by ER streaming.

    <bold>IMPORTANCE</bold>Intracellular trafficking is a primary and essential step for the cell-to-cell movement of viruses. To date, many studies have demonstrated the rapid intracellular movement of viral factors but have failed to provide evidence for the mechanism or biological significance of this motility. Here, we observed that agglomerates of nucleocapsid protein (NP) moved rapidly throughout the cell, and we performed live imaging and ultrastructural analysis to identify the mechanism of motility. We provide evidence that cytoplasmic protein agglomerates were passively dragged by actomyosin-mediated streaming of the endoplasmic reticulum (ER) in plant cells. In virus-infected cells, NP agglomerates were surrounded by the ER membranes, indicating that NP agglomerates form the basis of enveloped virus particles in close proximity to the ER. Our work provides a sophisticated model of macromolecular trafficking in plant cells and improves our understanding of the formation of enveloped particles of negative-strand RNA viruses.

    DOI: 10.1128/jvi.02527-14

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  • Complete nucleotide sequence and genome structure of a Japanese isolate of hibiscus latent Fort Pierce virus, a unique tobamovirus that contains an internal poly(A) region in its 3′ end 査読

    Tetsuya Yoshida, Yugo Kitazawa, Ken Komatsu, Yutaro Neriya, Kazuya Ishikawa, Naoko Fujita, Masayoshi Hashimoto, Kensaku Maejima, Yasuyuki Yamaji, Shigetou Namba

    Archives of Virology   159 ( 11 )   3161 - 3165   2014年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    In this study, we detected a Japanese isolate of hibiscus latent Fort Pierce virus (HLFPV-J), a member of the genus Tobamovirus, in a hibiscus plant in Japan and determined the complete sequence and organization of its genome. HLFPV-J has four open reading frames (ORFs), each of which shares more than 98 % nucleotide sequence identity with those of other HLFPV isolates. Moreover, HLFPV-J contains a unique internal poly(A) region of variable length, ranging from 44 to 78 nucleotides, in its 3'-untranslated region (UTR), as is the case with hibiscus latent Singapore virus (HLSV), another hibiscus-infecting tobamovirus. The length of the HLFPV-J genome was 6431 nucleotides, including the shortest internal poly(A) region. The sequence identities of ORFs 1, 2, 3 and 4 of HLFPV-J to other tobamoviruses were 46.6-68.7, 49.9-70.8, 31.0-70.8 and 39.4-70.1 %, respectively, at the nucleotide level and 39.8-75.0, 43.6-77.8, 19.2-70.4 and 31.2-74.2 %, respectively, at the amino acid level. The 5'- and 3'-UTRs of HLFPV-J showed 24.3-58.6 and 13.0-79.8 % identity, respectively, to other tobamoviruses. In particular, when compared to other tobamoviruses, each ORF and UTR of HLFPV-J showed the highest sequence identity to those of HLSV. Phylogenetic analysis showed that HLFPV-J, other HLFPV isolates and HLSV constitute a malvaceous-plant-infecting tobamovirus cluster. These results indicate that the genomic structure of HLFPV-J has unique features similar to those of HLSV. To our knowledge, this is the first report of the complete genome sequence of HLFPV.

    DOI: 10.1007/s00705-014-2175-3

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    その他リンク: http://link.springer.com/article/10.1007/s00705-014-2175-3/fulltext.html

  • Recognition of floral homeotic MADS domain transcription factors by a phytoplasmal effector, phyllogen, induces phyllody 査読

    Kensaku Maejima, Ryo Iwai, Misako Himeno, Ken Komatsu, Yugo Kitazawa, Naoko Fujita, Kazuya Ishikawa, Misato Fukuoka, Nami Minato, Yasuyuki Yamaji, Kenro Oshima, Shigetou Namba

    The Plant Journal   78 ( 4 )   541 - 554   2014年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

    DOI: 10.1111/tpj.12495

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    その他リンク: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/tpj.12495

  • Development of an on-site plum pox virus detection kit based on immunochromatography 査読

    Kensaku Maejima, Misako Himeno, Osamu Netsu, Kazuya Ishikawa, Tetsuya Yoshida, Naoko Fujita, Masayoshi Hashimoto, Ken Komatsu, Yasuyuki Yamaji, Shigetou Namba

    Journal of General Plant Pathology   80 ( 2 )   176 - 183   2014年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    Sharka disease, caused by plum pox virus (PPV), is the most serious viral disease of stone fruit trees. Among the eight known strains of the virus, PPV-D is the most important due to its recent global spread. Although enzyme-linked immunosorbent assay (ELISA) is the most common approach for diagnosing sharka, it involves time-consuming steps and requires expensive equipment and trained technicians. In this study, an on-site PPV detection kit based on immunochromatography was developed using polyclonal antibodies against the coat protein (CP) of a PPV-D isolate. The immunochromatographic (IC) assay kit was as sensitive as a commercial ELISA system for detecting Japanese PPV-D isolates. Moreover, it was easy to use (a one-step procedure), and results could be obtained on-site within 15 min without special laboratory equipment. The IC assay kit detected the virus from every aerial part of symptomatic Japanese apricot trees. In a detailed study of viral localization in leaves, the most suitable plant parts for use in the IC assay were symptomatic mesophyll tissues and the region from the petiole to the main vein. A positive reaction was also observed using the CP of other major (PPV-M and PPV-Rec) and minor (PPV-EA, PPV-W, and PPV-T) strains.

    DOI: 10.1007/s10327-014-0504-8

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    その他リンク: http://link.springer.com/article/10.1007/s10327-014-0504-8/fulltext.html

  • Fig mosaic emaravirus p4 protein is involved in cell-to-cell movement 査読

    Kazuya Ishikawa, Kensaku Maejima, Ken Komatsu, Osamu Netsu, Takuya Keima, Takuya Shiraishi, Yukari Okano, Masayoshi Hashimoto, Yasuyuki Yamaji, Shigetou Namba

    Journal of General Virology   94 ( 3 )   682 - 686   2013年3月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Microbiology Society  

    Fig mosaic virus (FMV), a member of the newly formed genus Emaravirus, is a segmented negative-strand RNA virus. Each of the six genomic FMV segments contains a single ORF: that of RNA4 encodes the protein p4. FMV-p4 is presumed to be the movement protein (MP) of the virus; however, direct experimental evidence for this is lacking. We assessed the intercellular distribution of FMV-p4 in plant cells by confocal laser scanning microscopy and we found that FMV-p4 was localized to plasmodesmata and to the plasma membrane accompanied by tubule-like structures. A series of experiments designed to examine the movement functions revealed that FMV-p4 has the capacity to complement viral cell-to-cell movement, prompt GFP diffusion between cells, and spread by itself to neighbouring cells. Altogether, our findings demonstrated that FMV-p4 shares several properties with other viral MPs and plays an important role in cell-to-cell movement.

    DOI: 10.1099/vir.0.047860-0

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  • Identification and characterization of two novel genomic RNA segments of fig mosaic virus, RNA5 and RNA6 査読

    Kazuya Ishikawa, Kensaku Maejima, Ken Komatsu, Yugo Kitazawa, Masayoshi Hashimoto, Daisuke Takata, Yasuyuki Yamaji, Shigetou Namba

    Journal of General Virology   93 ( 7 )   1612 - 1619   2012年7月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Microbiology Society  

    Fig mosaic virus (FMV), a negative-strand RNA virus, is recognized as a causal agent of fig mosaic disease. We performed RT-PCR for 14 FMV isolates collected from symptomatic fig plants in Japan and Serbia using primers corresponding to the conserved 13 nt stretches found at the termini of FMV genomic segments. The resulting simultaneous amplification of all FMV genomic segments yielded four previously identified segments of FMV and two novel segments. These novel FMV genomic RNA segments were found in each of the 14 FMV isolates analysed. In Northern blot studies, both the sense and antisense strands of these novel RNA molecules accumulated in FMV-infected fig leaves but not in uninfected fig leaves, confirming that they replicate as FMV genomic segments. Sequence analysis showed that the novel RNA segments are similar, in their structural organization and molecular evolutionary patterns, to those of known FMV genomic RNA segments. Our findings thus indicate that these newly discovered RNA segments are previously unidentified FMV genomic segments, which we have designated RNA5 and RNA6.

    DOI: 10.1099/vir.0.042663-0

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  • Infection of capilloviruses requires subgenomic RNAs whose transcription is controlled by promoter-like sequences conserved among flexiviruses 査読

    Ken Komatsu, Hisae Hirata, Takako Fukagawa, Yasuyuki Yamaji, Yukari Okano, Kazuya Ishikawa, Tatsushi Adachi, Kensaku Maejima, Masayoshi Hashimoto, Shigetou Namba

    Virus Research   167 ( 1 )   8 - 15   2012年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.virusres.2012.02.019

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  • First report of fig mosaic virus infecting common fig (Ficus carica) in Japan 査読

    ISHIKAWA Kazuya, MAEJIMA Kensaku, NAGASHIMA Susumu, SAWAMURA Nobuo, TAKINAMI Yusuke, KOMATSU Ken, HASHIMOTO Masayoshi, YAMAJI Yasuyuki, YAMAMOTO Jun, NAMBA Shigetou

    Journal of general plant pathology : JGPP   78 ( 2 )   136 - 139   2012年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/s10327-012-0359-9

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    その他リンク: http://link.springer.com/article/10.1007/s10327-012-0359-9/fulltext.html

  • Identification of three MAPKKKs forming a linear signaling pathway leading to programmed cell death in Nicotiana benthamiana 査読

    Masayoshi Hashimoto, Ken Komatsu, Kensaku Maejima, Yukari Okano, Takuya Shiraishi, Kazuya Ishikawa, Yusuke Takinami, Yasuyuki Yamaji, Shigetou Namba

    BMC Plant Biology   12 ( 1 )   103 - 103   2012年

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

    DOI: 10.1186/1471-2229-12-103

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MISC

  • オルガネラグルー技術による植物メタボロームの人為操作 招待 査読

    石川 一也, 児玉 豊

    生物物理   63 ( 5 )   247 - 251   2023年9月

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  • ER 膜屈曲の調節因子であるレティキュロンはゼニゴケにおいて葉緑体定位運動を促進する

    石川 一也, 今野 涼太, 藤井 雄太, 藤原 正幸, 深尾 陽一朗, 児玉 豊

    日本植物生理学会年会(Web) 63th   2022年

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    担当区分:筆頭著者  

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  • 蛍光バイオセンサーUnaGを用いた植物ビリルビンの生体内分布と生合成経路の解析

    石川一也, 謝肖男, 宮脇敦史, 沼田圭司, 児玉豊

    日本植物生理学会年会(Web) 62th   2021年

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    担当区分:筆頭著者  

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  • リンゴ小球形潜在ウイルス(ALSV)を利用した薬用植物ムラサキのウイルス誘導性ジーンサイレンシング

    草野博彰, 出石佑樹, 井坂夏海, 李豪, 中西浩平, 影山丈士, 石川一也, 嶋田知生, 増田税, 吉川信幸, 矢崎一史

    イソプレノイド研究会例会講演要旨集   30th   2020年

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  • 蛍光バイオセンサーUnaGを用いた植物ビリルビンのライブセルイメージング

    石川一也, 謝肖男, 宮脇敦史, 沼田圭司, 児玉豊

    日本植物生理学会年会(Web) 61th   2020年

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    担当区分:筆頭著者  

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  • 小胞体-細胞膜接着部位の構成因子の同定と機能解析

    石川一也, 田村謙太郎, 深尾陽一朗, 嶋田知生

    日本植物生理学会年会(Web)   60th   2019年

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  • 細胞膜接着部位に着目した小胞体の4次元構造解析

    石川一也, 田村謙太郎, 上田晴子, 伊藤容子, 中野明彦, 中野明彦, 西村いくこ, 嶋田知生

    日本植物生理学会年会(Web)   59th   2018年

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  • ファイトプラズマ感染が花の形態形成遺伝子の発現へ与える影響

    姫野 未紗子, 前島 健作, 湊 菜未, 煉谷 裕太朗, 三浦 千裕, 石川 一也, 桂馬 拓也, 大島 研郎, 難波 成任

    日本マイコプラズマ学会雑誌 = Japanese Journal of Mycoplasmology   39   30 - 33   2013年3月

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  • ファイトプラズマのポストゲノム生物学 : 宿主スイッチングにおける網羅的遺伝子発現解析

    OSHIMA Kenro, MIURA Chihiro, MINATO Nami, NERIYA Yutaro, ISHIKAWA Kazuya, KITAZAWA Yugo, NIJO Takamichi, WATANABE Tsubasa, MAEJIMA Kensaku, NAMBA Shigetou

    日本マイコプラズマ学会雑誌 = Japanese Journal of Mycoplasmology   39   1 - 3   2013年3月

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受賞

  • 研究科長賞受賞

    2016年3月   東京大学農学生命科学研究科   博士論文「イチジクモザイクウイルスのタンパク質機能に関する細胞生物学的研究」

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  • 総長賞

    2013年3月   東京大学   修士論文「ダニに媒介されるエマラウイルスゲノムの構造と機能の解析ならびに遺伝子診断法の開発に関する研究」

    石川一也

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  • 研究科長賞受賞

    2013年3月   東京大学農学生命科学研究科   修士論文「ダニに媒介されるエマラウイルスゲノムの構造と機能の解析ならびに遺伝子診断法の開発に関する研究」

    石川一也

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共同研究・競争的資金等の研究

  • 広範な病害抵抗性開発に向けたER-細胞膜接着部位と細菌の相互作用機構の解明

    研究課題/領域番号:22K14892  2022年04月 - 2024年03月

    日本学術振興会  科学研究費助成事業  若手研究

    石川 一也

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    配分額:4550000円 ( 直接経費:3500000円 、 間接経費:1050000円 )

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担当授業科目

  • 創薬研究を支える生命科学 (2023年度) 第3学期  - 木5~6

  • 薬学基本実習 (2023年度) 第1学期  - その他5~9

  • 薬学基本実習 (2023年度) 第1学期  - その他5~9

  • 薬学基本実習 (2023年度) 第1学期  - その他5~9

  • 薬学基本実習 (2023年度) 第1学期  - その他5~9

  • 衛生薬学実習 (2023年度) 2・3学期  - その他5~9

  • 衛生薬学実習 (2023年度) 2・3学期  - その他5~9

  • 衛生薬学実習 (2023年度) 2・3学期  - その他5~9

  • 衛生薬学実習 (2023年度) 2・3学期  - その他5~9

  • 薬学基本実習 (2022年度) 第1学期  - その他5~9

  • 薬学基本実習 (2022年度) 第1学期  - その他5~9

  • 衛生薬学実習 (2022年度) 2・3学期  - その他5~9

  • 衛生薬学実習 (2022年度) 2・3学期  - その他5~9

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