Updated on 2025/06/06

写真a

 
BANDO Tetsuya
 
Organization
Faculty of Medicine, Dentistry and Pharmaceutical Sciences Lecturer
Position
Lecturer
External link

Degree

  • Ph.D. (Science) ( Okayama University )

Research Interests

  • 器官再生

  • Regeneration

Research Areas

  • Life Science / Genome biology

  • Life Science / Developmental biology

  • Life Science / System genome science

Education

  • Okayama University   自然科学研究科   生物資源科学専攻

    - 2005

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    Country： Japan

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  • Okayama University   理学研究科   生物学専攻

    - 1999

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    Country： Japan

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  • Okayama University   理学部   生物学科

    - 1997

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    Country： Japan

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Research History

  • - Senior Assistant Professor,Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,Okayama University

    2016

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  • - 岡山大学医歯薬学総合研究科 講師

    2016

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  • Assistant Professor,Graduate School of Medicine, Dentistry and Pharmaceutical Sciences,Okayama University

    2012 - 2016

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  • 岡山大学医歯薬学総合研究科 助教

    2012 - 2016

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  • Researcher

    2009 - 2012

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  • The University of Tokushima

    2009 - 2012

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  • Researcher

    2008 - 2009

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  • 科学技術振興機構 研究員

    2008 - 2009

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  • Researcher

    2005 - 2008

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  • The University of Tokushima

    2005 - 2008

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Professional Memberships

 

Papers

  • Toll signalling promotes blastema cell proliferation during cricket leg regeneration via insect macrophages Reviewed International journal

    Tetsuya Bando, Misa Okumura, Yuki Bando, Marou Hagiwara, Yoshimasa Hamada, Yoshiyasu Ishimaru, Taro Mito, Eri Kawaguchi, Takeshi Inoue, Kiyokazu Agata, Sumihare Noji, Hideyo Ohuchi

    Development   149 ( 8 )   2022.4

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：The Company of Biologists  

    <title>ABSTRACT</title>
    Hemimetabolous insects, such as the two-spotted cricket Gryllus bimaculatus, can recover lost tissues, in contrast to the limited regenerative abilities of human tissues. Following cricket leg amputation, the wound surface is covered by the wound epidermis, and plasmatocytes, which are insect macrophages, accumulate in the wound region. Here, we studied the function of Toll-related molecules identified by comparative RNA sequencing during leg regeneration. Of the 11 Toll genes in the Gryllus genome, expression of Toll2-1, Toll2-2 and Toll2-5 was upregulated during regeneration. RNA interference (RNAi) of Toll, Toll2-1, Toll2-2, Toll2-3 or Toll2-4 produced regeneration defects in more than 50% of crickets. RNAi of Toll2-2 led to a decrease in the ratio of S- and M-phase cells, reduced expression of JAK/STAT signalling genes, and reduced accumulation of plasmatocytes in the blastema. Depletion of plasmatocytes in crickets using clodronate also produced regeneration defects, as well as fewer proliferating cells in the regenerating legs. Plasmatocyte depletion also downregulated the expression of Toll and JAK/STAT signalling genes in the regenerating legs. These results suggest that Spz-Toll-related signalling in plasmatocytes promotes leg regeneration through blastema cell proliferation by regulating the Upd-JAK/STAT signalling pathway.

    DOI： 10.1242/dev.199916

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    Other Link： https://journals.biologists.com/dev/article-pdf/doi/10.1242/dev.199916/2119087/dev199916.pdf

  • Insights into the genomic evolution of insects from cricket genomes Reviewed International coauthorship International journal

    Guillem Ylla, Taro Nakamura, Takehiko Itoh, Rei Kajitani, Atsushi Toyoda, Sayuri Tomonari, Tetsuya Bando, Yoshiyasu Ishimaru, Takahito Watanabe, Masao Fuketa, Yuji Matsuoka, Austen A. Barnett, Sumihare Noji, Taro Mito, Cassandra G. Extavour

    Communications Biology   4 ( 1 )   2021.12

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    <title>Abstract</title>Most of our knowledge of insect genomes comes from Holometabolous species, which undergo complete metamorphosis and have genomes typically under 2 Gb with little signs of DNA methylation. In contrast, Hemimetabolous insects undergo the presumed ancestral process of incomplete metamorphosis, and have larger genomes with high levels of DNA methylation. Hemimetabolous species from the Orthopteran order (grasshoppers and crickets) have some of the largest known insect genomes. What drives the evolution of these unusual insect genome sizes, remains unknown. Here we report the sequencing, assembly and annotation of the 1.66-Gb genome of the Mediterranean field cricket <italic>Gryllus bimaculatus</italic>, and the annotation of the 1.60-Gb genome of the Hawaiian cricket <italic>Laupala kohalensis</italic>. We compare these two cricket genomes with those of 14 additional insects and find evidence that hemimetabolous genomes expanded due to transposable element activity. Based on the ratio of observed to expected CpG sites, we find higher conservation and stronger purifying selection of methylated genes than non-methylated genes. Finally, our analysis suggests an expansion of the <italic>pickpocket</italic> class V gene family in crickets, which we speculate might play a role in the evolution of cricket courtship, including their characteristic chirping.

    DOI： 10.1038/s42003-021-02197-9

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    Other Link： http://www.nature.com/articles/s42003-021-02197-9

  • Molecular mechanisms of limb regeneration: insights from regenerating legs of the cricket Gryllus bimaculatus. Reviewed International journal

    Tetsuya Bando, Taro Mito, Yoshimasa Hamada, Yoshiyasu Ishimaru, Sumihare Noji, Hideyo Ohuchi

    The International journal of developmental biology   62 ( 6-7-8 )   559 - 569   2018

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    Language：English   Publishing type：Research paper (scientific journal)  

    This review summarizes recent advances in leg regeneration research, focusing on the cricket Gryllus bimaculatus. Recent studies have revealed molecular mechanisms on blastema formation, establishment of positional information, and epigenetic regulation during leg regeneration. Especially, these studies have provided molecular bases in classical conceptual models such as the polar coordinate model, the intercalation model, the boundary model, the steepness model, etc., which were proposed to interpret regeneration processes of the cockroach legs. When a leg is amputated, a blastema is formed through the activation of the Janus-kinase (Jak)/Signal-Transduction-and-Activator-of-Transcription (STAT) pathway. Subsequently, the Hedgehog/Wingless/Decapentaplegic/Epidermal-growth-factor pathways instruct distalization in the blastema, designated as the molecular boundary model. Downstream targets of this pathway are transcription factors Distal-less (Dll) and dachshund (dac), functioning as key regulators of proximodistal pattern formation. Dll and dac specify the distal and proximal regions in the blastema, respectively, through the regulation of tarsal patterning genes. The expression of leg patterning genes during regeneration may be epigenetically controlled by histone H3K27 methylation via Enhancer-of-zeste and Ubiquitously-transcribed-tetratricopeptide-repeat-gene-X-chromosome. For the molecular mechanism of intercalation of the missing structures between the amputated position and the most distal one, Dachsous/Fat (Ds/Ft) steepness model has been proposed, in which the Ds/Ft pathway maintains positional information and determines leg size through dac expression. This model was theoretically verified to interpret the experimental results obtained with cricket legs. Availability of whole-genome sequence information, regeneration-dependent RNA interference, and genome editing technique will have the cricket be an ideal model system to reveal gene functions in leg regeneration.

    DOI： 10.1387/ijdb.180048ho

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  • Leg formation and regeneration Reviewed

    Tetsuya Bando, Yoshimasa Hamada, Sumihare Noji

    The Cricket as a Model Organism: Development, Regeneration, and Behavior   31 - 48   2017.1

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    Publishing type：Part of collection (book)  

    DOI： 10.1007/978-4-431-56478-2_3

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  • Enhancer of zeste plays an important role in photoperiodic modulation of locomotor rhythm in the cricket, Gryllus bimaculatus. International journal

    Yoshimasa Hamada, Atsushi Tokuoka, Tetsuya Bando, Hideyo Ohuchi, Kenji Tomioka

    Zoological letters   2   5 - 5   2016

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    INTRODUCTION: Insects show daily behavioral rhythms controlled by an endogenous oscillator, the circadian clock. The rhythm synchronizes to daily light-dark cycles (LD) and changes waveform in association with seasonal change in photoperiod. RESULTS: To explore the molecular basis of the photoperiod-dependent changes in circadian locomotor rhythm, we investigated the role of a chromatin modifier, Enhancer of zeste (Gb'E(z)), in the cricket, Gryllus bimaculatus. Under a 12 h:12 h LD (LD 12:12), Gb'E(z) was constitutively expressed in the optic lobe, the site of the biological clock; active phase (α) and rest phase (ρ) were approximately 12 h in duration, and α/ρ ratio was approximately 1.0. When transferred to LD 20:4, the α/ρ ratio decreased significantly, and the Gb'E(z) expression level was significantly reduced at 6 h and 10 h after light-on, as was reflected in the reduced level of trimethylation of histone H3 lysine 27. This change was associated with change in clock gene expression profiles. The photoperiod-dependent changes in α/ρ ratio and clock gene expression profiles were prevented by knocking down Gb'E(z) by RNAi. CONCLUSIONS: These results suggest that histone modification by Gb'E(z) is involved in photoperiodic modulation of the G. bimaculatus circadian rhythm.

    DOI： 10.1186/s40851-016-0042-7

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  • Short germ insects utilize both the ancestral and derived mode of Polycomb group-mediated epigenetic silencing of Hox genes

    Yuji Matsuoka, Tetsuya Bando, Takahito Watanabe, Yoshiyasu Ishimaru, Sumihare Noji, Aleksandar Popadic, Taro Mito

    BIOLOGY OPEN   4 ( 6 )   702 - 709   2015.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1242/bio.201411064

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  • Leg regeneration is epigenetically regulated by histone H3K27 methylation in the cricket Gryllus bimaculatus

    Hamada Yoshimasa, Bando Tetsuya, Nakamura Taro, Ishimaru Yoshiyasu, Mito Taro, Noji Sumihare, Tomioka Kenji, Ohuchi Hideyo

    Development   142 ( 17 )   2916 - 2927   2015

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    Language：English   Publisher：Company of Biologists  

    DOI： 10.1242/dev

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  • Analysis of RNA-Seq data reveals involvement of JAK/STAT signalling during leg regeneration in the cricket Gryllus bimaculatus

    Tetsuya Bando, Yoshiyasu Ishimaru, Takuro Kida, Yoshimasa Hamada, Yuji Matsuoka, Taro Nakamura, Hideyo Ohuchi, Sumihare Noji, Taro Mito

    DEVELOPMENT   140 ( 5 )   959 - 964   2013.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1242/dev.084590

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  • Lowfat, a Mammalian Lix1 Homologue, Regulates Leg Size and Growth Under the Dachsous/Fat Signaling Pathway During Tissue Regeneration Reviewed

    Tetsuya Bando, Yoshimasa Hamada, Kazuki Kurita, Taro Nakamura, Taro Mito, Hideyo Ohuchi, Sumihare Noji

    DEVELOPMENTAL DYNAMICS   240 ( 6 )   1440 - 1453   2011.6

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    DOI： 10.1002/dvdy.22647

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  • Regulation of leg size and shape by the Dachsous/Fat signalling pathway during regeneration Reviewed

    Tetsuya Bando, Taro Mito, Yuko Maeda, Taro Nakamura, Fumiaki Ito, Takahito Watanabe, Hideyo Ohuchi, Sumihare Noji

    DEVELOPMENT   136 ( 13 )   2235 - 2245   2009.7

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    DOI： 10.1242/dev.035204

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  • The homeoproteins MAB-18 and CEH-14 insulate the dauer collagen gene col-43 from activation by the adjacent promoter of the Spermatheca gene sth-1 in Caenorhabditis elegans. International journal

    Tetsuya Bando, Tatsuji Ikeda, Hiroaki Kagawa

    Journal of molecular biology   348 ( 1 )   101 - 12   2005.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    Genome searches in this study indicate that the nematode Caenorhabditis elegans genome has 2582 bidirectionally oriented genes that account for more than 25% of the total genes. We analyze the transcriptional repression system for one of these predicted bidirectional promoters, which controls the expression of the spermathecal gene sth-1 and collagen gene col-43. These two genes are separated by 1.3 kb and are transcribed bidirectionally. sth-1 is expressed in spermatheca after the L4 stage and col-43 is expressed in the hypodermal cells of the L2d dauer stage. The upstream regions required for the expression of sth-1 and col-43 shared an overlapped control sequence. Two homeoproteins, MAB-18 and CEH-14, were isolated by yeast one-hybrid screening as binding proteins of the overlapped region. MAB-18 bound to two homeodomain-binding sites and interacted with CEH-14 to repress col-43 expression in spermatheca. These results indicate that the two homeoproteins interact with each other to repress col-43 expression in sth-1-expressing tissues. This is the first report of bidirectional gene regulation analysis in the C.elegans genome.

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  • Involvement of a Basic Helix-Loop-Helix Gene BHLHE40 in Specification of Chicken Retinal Pigment Epithelium Reviewed International journal

    Toshiki Kinuhata, Keita Sato, Tetsuya Bando, Taro Mito, Satoru Miyaishi, Tsutomu Nohno, Hideyo Ohuchi

    Journal of Developmental Biology   10 ( 4 )   45 - 45   2022.10

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

    The first event of differentiation and morphogenesis in the optic vesicle (OV) is specification of the neural retina (NR) and retinal pigment epithelium (RPE), separating the inner and outer layers of the optic cup, respectively. Here, we focus on a basic helix-loop-helix gene, BHLHE40, which has been shown to be expressed by the developing RPE in mice and zebrafish. Firstly, we examined the expression pattern of BHLHE40 in the developing chicken eye primordia by in situ hybridization. Secondly, BHLHE40 overexpression was performed with in ovo electroporation and its effects on optic cup morphology and expression of NR and RPE marker genes were examined. Thirdly, we examined the expression pattern of BHLHE40 in LHX1-overexpressed optic cup. BHLHE40 expression emerged in a subset of cells of the OV at Hamburger and Hamilton stage 14 and became confined to the outer layer of the OV and the ciliary marginal zone of the retina by stage 17. BHLHE40 overexpression in the prospective NR resulted in ectopic induction of OTX2 and repression of VSX2. Conversely, BHLHE40 was repressed in the second NR after LHX1 overexpression. These results suggest that emergence of BHLHE40 expression in the OV is involved in initial RPE specification and that BHLHE40 plays a role in separation of the early OV domains by maintaining OTX2 expression and antagonizing an NR developmental program.

    DOI： 10.3390/jdb10040045

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  • The role of clockwork orange in the circadian clock of the cricket Gryllus bimaculatus Reviewed International journal

    Yasuaki Tomiyama, Tsugumichi Shinohara, Mirai Matsuka, Tetsuya Bando, Taro Mito, Kenji Tomioka

    Zoological Letters   6 ( 1 )   2020.12

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    <title>Abstract</title>
    The circadian clock generates rhythms of approximately 24 h through periodic expression of the clock genes. In insects, the major clock genes <italic>period</italic> (<italic>per</italic>) and <italic>timeless</italic> (<italic>tim</italic>) are rhythmically expressed upon their transactivation by CLOCK/CYCLE, with peak levels in the early night. In <italic>Drosophila</italic>, <italic>clockwork orange</italic> (<italic>cwo</italic>) is known to inhibit the transcription of <italic>per</italic> and <italic>tim</italic> during the daytime to enhance the amplitude of the rhythm, but its function in other insects is largely unknown. In this study, we investigated the role of <italic>cwo</italic> in the clock mechanism of the cricket <italic>Gryllus bimaculatus</italic>. The results of quantitative RT-PCR showed that under a light/dark (LD) cycle, <italic>cwo</italic> is rhythmically expressed in the optic lobe (lamina-medulla complex) and peaks during the night. When <italic>cwo</italic> was knocked down via RNA interference (RNAi), some crickets lost their locomotor rhythm, while others maintained a rhythm but exhibited a longer free-running period under constant darkness (DD). In <italic>cwo</italic>RNAi crickets, all clock genes except for <italic>cryptochrome 2</italic> (<italic>cry2</italic>) showed arrhythmic expression under DD; under LD, some of the clock genes showed higher mRNA levels, and <italic>tim</italic> showed rhythmic expression with a delayed phase. Based on these results, we propose that <italic>cwo</italic> plays an important role in the cricket circadian clock.

    DOI： 10.1186/s40851-020-00166-4

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    Other Link： http://link.springer.com/article/10.1186/s40851-020-00166-4/fulltext.html

  • Functional analysis of Pdp1 and vrille in the circadian system of a cricket

    Yumina Narasaki-Funo, Yasuaki Tomiyama, Motoki Nose, Tetsuya Bando, Kenji Tomioka

    Journal of Insect Physiology   127   2020.11

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier Ltd  

    DOI： 10.1016/j.jinsphys.2020.104156

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  • Fgf10-CRISPR mosaic mutants demonstrate the gene dose-related loss of the accessory lobe and decrease in the number of alveolar type 2 epithelial cells in mouse lung Reviewed International journal

    Munenori Habuta, Akihiro Yasue, Ken-ichi T. Suzuki, Hirofumi Fujita, Keita Sato, Hitomi Kono, Ayuko Takayama, Tetsuya Bando, Satoru Miyaishi, Seiichi Oyadomari, Eiji Tanaka, Hideyo Ohuchi

    PLOS ONE   15 ( 10 )   e0240333 - e0240333   2020.10

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Public Library of Science (PLoS)  

    DOI： 10.1371/journal.pone.0240333

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  • Dkk3/REIC, an N-glycosylated Protein, Is a Physiological Endoplasmic Reticulum Stress Inducer in the Mouse Adrenal Gland. Reviewed

    Hirofumi Fujita, Tetsuya Bando, Seiichi Oyadomari, Kazuhiko Ochiai, Masami Watanabe, Hiromi Kumon, Hideyo Ohuchi

    Acta medica Okayama   74 ( 3 )   199 - 208   2020.6

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    Dickkopf 3 (Dkk3) is a secreted protein belonging to the Dkk family and encoded by the orthologous gene of REIC. Dkk3/REIC is expressed by mouse and human adrenal glands, but the understanding of its roles in this organ is still limited. To determine the functions of Dkk3 in the mouse adrenal gland, we first identified that the mouse Dkk3 protein is N-glycosylated in the adrenal gland as well as in the brain. We performed proteome analysis on adrenal glands from Dkk3-null mice, in which exons 5 and 6 of the Dkk3 gene are deleted. Twodimensional polyacrylamide gel electrophoresis of adrenal proteins from wild-type and Dkk3-null mice revealed 5 protein spots whose intensities were altered between the 2 genotypes. Mass spectrometry analysis of these spots identified binding immunoglobulin protein (BiP), an endoplasmic reticulum (ER) chaperone. To determine whether mouse Dkk3 is involved in the unfolded protein response (UPR), we carried out a reporter assay using ER-stress responsive elements. Forced expression of Dkk3 resulted in the induction of distinct levels of reporter expression, showing the UPR initiated by the ER membrane proteins of activating transcription factor 6 (ATF6) and inositol-requring enzyme 1 (IRE1). Thus, it is possible that Dkk3 is a physiological ER stressor in the mouse adrenal gland.

    DOI： 10.18926/AMO/59950

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  • Dickkopf3 (Dkk3) is required for maintaining the integrity of secretory vesicles in the mouse adrenal medulla. Reviewed International journal

    Habuta M, Fujita H, Sato K, Bando T, Inoue J, Kondo Y, Miyaishi S, Kumon H, Ohuchi H

    Cell and tissue research   379 ( 1 )   157 - 167   2019.10

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    REIC (reduced expression in immortalized cells) has been identified as a gene whose expression was reduced in immortalized cultured cells. The REIC gene is identical to Dickkopf-3 (Dkk3), which encodes a secreted glycoprotein belonging to the Dkk family. Previously, we showed that Dkk3 protein is present in the mouse adrenal medulla. However, its role in this tissue has not been elucidated. To explore it, we performed electron microscopic (EM) studies and RNA-sequencing (RNA-seq) analysis on Dkk3-null adrenal glands. EM studies showed that the number of dense core secretory vesicles were significantly reduced and empty vesicles were increased in the medulla endocrine cells. Quantitative PCR (qPCR) analysis showed relative expression levels of chromogranin A (Chga) and neuropeptide Y (Npy) were slightly but significantly reduced in the Dkk3-null adrenal glands. From the result of RNA-seq analysis as a parallel study, we selected three of the downregulated genes, uncoupled protein-1 (Ucp1), growth arrest and DNA-damage-inducible 45 gamma (Gadd45g), and Junb with regard to the estimated expression levels. In situ hybridization confirmed that these genes were regionally expressed in the adrenal gland. However, expression levels of these three genes were not consistent as revealed by qPCR. Thus, Dkk3 maintains the integrity of secreting vesicles in mouse adrenal medulla by regulating the expression of Chga and Npy.

    DOI： 10.1007/s00441-019-03113-8

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  • Localization of the ultraviolet-sensor Opn5m and its effect on myopia-related gene expression in the late-embryonic chick eye. Reviewed International journal

    Kato M, Sato K, Habuta M, Fujita H, Bando T, Morizane Y, Shiraga F, Miyaishi S, Ohuchi H

    Biochemistry and biophysics reports   19   100665 - 100665   2019.9

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    Recent studies show that exposure to ultraviolet (UV) light suppresses ocular elongation, which causes myopia development. However, the specific mechanisms of this process have not been elucidated. A UV-sensor, Opsin 5 (Opn5) mRNA was shown to be present in extraretinal tissues. To test the possibility that UV-signals mediated by Opn5 would have a direct effect on the outer connective tissues of the eye, we first examined the expression patterns of a mammalian type Opn5 (Opn5m) in the late-embryonic chicken eye. Quantitative PCR showed Opn5m mRNA expression in the cornea and sclera. The anti-Opn5m antibody stained a small subset of cells in the corneal stroma and fibrous sclera. We next assessed the effect of UV-A (375 nm) irradiation on the chicken fibroblast cell line DF-1 overexpressing chicken Opn5m. UV-A irradiation for 30 min significantly increased the expression of Early growth response 1 (Egr1), known as an immediate early responsive gene, and of Matrix metalloproteinase 2 (Mmp2) in the presence of retinal chromophore 11-cis-retinal. In contrast, expression of Transforming growth factor beta 2 and Tissue inhibitor of metalloproteinase 2 was not significantly altered. These results indicate that UV-A absorption by Opn5m can upregulate the expression levels of Egr1 and Mmp2 in non-neuronal, fibroblasts. Taken together with the presence of Opn5m in the cornea and sclera, it is suggested that UV-A signaling mediated by Opn5 in the extraretinal ocular tissues could influence directly the outer connective tissues of the chicken late-embryonic eye.

    DOI： 10.1016/j.bbrep.2019.100665

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  • A novel photic entrainment mechanism for the circadian clock in an insect: involvement of c-fos and cryptochromes Reviewed

    Kutaragi Y, Tokuoka A, Tomiyama Y, Nose M, Watanabe T, Bando T, Moriyama Y, Tomioka K

    Zoological Letter   4   26   2018.9

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    Background | Entrainment to the environmental light cycle is an essential property of the circadian clock. Although the compound eye is known to be the major photoreceptor necessary for entrainment in many insects, the molecular mechanisms of photic entrainment remain to be explored.<br />
    Results | We found that cryptochromes (crys) and c-fos mediate photic entrainment of the circadian clock in a hemimetabolous insect, the cricket Gryllus bimaculatus. We examined the effects of RNA interference (RNAi)-mediated knockdown of the cry genes, Gb’cry1 and Gb’cry2, on photic entrainment, and light-induced resetting of the circadian locomotor rhythm. Gb’cry2 RNAi accelerated entrainment for delay shifts, while Gb’cry1/ Gb’cry2 double RNAi resulted in significant lengthening of transient cycles in both advance and delay shifts, and even in entrainment failure in some crickets. Double RNAi also strongly suppressed light induced resetting. The Gb’cry-mediated phase shift or resetting of the rhythm was preceded by light-induced Gb’c-fosB expression. We also found that Gb’c-fosB, Gb’cry2 and Gb’period (Gb’per) were likely co-expressed in some optic lobe neurons.<br />
    Conclusion | Based on these results, we propose a novel model for photic entrainment of the insect circadian clock, which relies on the light information perceived by the compound eye.

    DOI： 10.1186/s40851-018-0109-8.

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  • Bone morphogenetic protein signaling in distal patterning and intercalation during leg regeneration of the cricket, Gryllus bimaculatus. Reviewed

    Ishimaru Y, Bando T, Ohuchi H, Noji S, Mito T

    Development, growth & differentiation   60 ( 6 )   377 - 386   2018.8

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  • Congenital eye anomalies: More mosaic than thought? Reviewed International journal

    Ohuchi H, Sato K, Habuta M, Fujita H, Bando T

    Congenital anomalies   59 ( 3 )   56 - 73   2018.7

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    The eye is a sensory organ that primarily captures light and provides the sense of sight, as well as delivering non-visual light information involving biological rhythms and neurophysiological activities to the brain. Since the early 1990s, rapid advances in molecular biology have enabled the identification of developmental genes, genes responsible for human congenital diseases, and relevant genes of mutant animals with various anomalies. In this review, we first look at the development of the eye, and we highlight seminal reports regarding archetypal gene defects underlying three developmental ocular disorders in humans: (1) holoprosencephaly (HPE), with cyclopia being exhibited in the most severe cases; (2) microphthalmia, anophthalmia, and coloboma (MAC) phenotypes; and (3) anterior segment dysgenesis (ASDG), known as Peters anomaly and its related disorders. The recently developed methods, such as next-generation sequencing and genome editing techniques, have aided the discovery of gene mutations in congenital eye diseases and gene functions in normal eye development. Finally, we discuss Pax6-genome edited mosaic eyes and propose that somatic mosaicism in developmental gene mutations should be considered a causal factor for variable phenotypes, sporadic cases, and de novo mutations in human developmental disorders.

    DOI： 10.1111/cga.12304

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  • timeless2 plays an important role in reproduction and circadian rhythms in the cricket Gryllus bimaculatus

    Motoki Nose, Atsushi Tokuoka, Tetsuya Bando, Kenji Tomioka

    Journal of Insect Physiology   105   9 - 17   2018.2

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier Ltd  

    DOI： 10.1016/j.jinsphys.2017.12.007

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  • A novel photic entrainment mechanism for the circadian clock in an insect: involvement of c-fos and cryptochromes

    Yuki Kutaragi, Atsushi Tokuoka, Yasuaki Tomiyama, Motoki Nose, Takayuki Watanabe, Tetsuya Bando, Yoshiyuki Moriyama, Kenji Tomioka

    Zoological Letters   4   2018

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  • Analysis of CRISPR genome-edited founder mice shows relationship between somatic mosaicism of Pax6 mutation and variable developmental eye abnormalities

    Akihiro Yasue, Hitomi Kono, Munenori Habuta, Tetsuya Bando, Keita Sato, Junji Inoue, Seiichi Oyadomari, Sumihare Noji, Eiji Tanaka, Hideyo Ohuchi

    MECHANISMS OF DEVELOPMENT   145   S151 - S151   2017.7

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  • cryptochrome genes form an oscillatory loop independent of the per/tim loop in the circadian clockwork of the cricket Gryllus bimaculatus

    Tokuoka, Atsushi, Itoh, Taichi Q., Hori, Shinryo, Uryu, Outa, Danbara, Yoshiki, Nose, Motoki, Bando, Tetsuya, Tanimura, Teiichi, Tomioka, Kenji

    ZOOLOGICAL LETTERS   3 ( 1 )   375 - 381   2017.4

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    DOI： 10.1186/s40851-017-0066-7

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  • Expression analysis of Dickkopf-related protein 3 (Dkk3) suggests its pleiotropic roles for a secretory glycoprotein in adult mouse

    Junji Inoue, Hirofumi Fujita, Tetsuya Bando, Yoichi Kondo, Hiromi Kumon, Hideyo Ohuchi

    JOURNAL OF MOLECULAR HISTOLOGY   48 ( 1 )   29 - 39   2017.2

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    DOI： 10.1007/s10735-016-9703-2

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  • Eye development and photoreception of a hemimetabolous insect, gryllus bimaculatus Reviewed

    Hideyo Ohuchi, Tetsuya Bando, Taro Mito, Sumihare Noji

    The Cricket as a Model Organism: Development, Regeneration, and Behavior   49 - 62   2017.1

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    Language：English   Publishing type：Part of collection (book)   Publisher：Springer Japan  

    DOI： 10.1007/978-4-431-56478-2_4

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  • Relationship between somatic mosaicism of Pax6 mutation and variable developmental eye abnormalities-an analysis of CRISPR genome-edited mouse embryos

    Akihiro Yasue, Hitomi Kono, Munenori Habuta, Tetsuya Bando, Keita Sato, Junji Inoue, Seiichi Oyadomari, Sumihare Noji, Eiji Tanaka, Hideyo Ohuchi

    Scientific Reports   7 ( 1 )   1 - 13   2017

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    DOI： 10.1038/s41598-017-00088-w

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  • Transcriptional and nontranscriptional events are involved in photic entrainment of the circadian clock in the cricket Gryllus bimaculatus

    Yuki Kutaragi, Taiki Miki, Tetsuya Bando, Kenji Tomioka

    PHYSIOLOGICAL ENTOMOLOGY   41 ( 4 )   358 - 368   2016.12

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    DOI： 10.1111/phen.12162

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  • TGF-beta signaling in insects regulates metamorphosis via juvenile hormone biosynthesis

    Yoshiyasu Ishimaru, Sayuri Tomonari, Yuji Matsuoka, Takahito Watanabe, Katsuyuki Miyawaki, Tetsuya Bando, Kenji Tomioka, Hideyo Ohuchi, Sumihare Noji, Taro Mito

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   113 ( 20 )   5634 - 5639   2016.5

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    DOI： 10.1073/pnas.1600612113

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  • Autopsy Case of Bilateral Optic Nerve Aplasia with Microphthalmia: Neural Retina Formation Is Required for the Coordinated Development of Ocular Tissues

    Hideyo Ohuchi, Kaori Taniguchi, Satoru Miyaishi, Hitomi Kono, Hirofumi Fujita, Tetsuya Bando, Chiharu Fuchizawa, Yuko Ohtani, Osamu Ohtani

    ACTA MEDICA OKAYAMA   70 ( 2 )   131 - 137   2016.4

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    DOI： 10.18926/AMO/54192

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  • Involvement of dachshund and Distal-less in distal pattern formation of the cricket leg during regeneration

    Yoshiyasu Ishimaru, Taro Nakamura, Tetsuya Bando, Yuji Matsuoka, Hideyo Ohuchi, Sumihare Noji, Taro Mito

    SCIENTIFIC REPORTS   5   8387   2015.2

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    DOI： 10.1038/srep08387

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  • An extended steepness model for leg-size determination based on Dachsous/Fat trans- dimer system

    Hiroshi Yoshida, Tetsuya Bando, Taro Mito, Hideyo Ohuchi, Sumihare Noji

    SCIENTIFIC REPORTS   4   4335   2014.3

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    DOI： 10.1038/srep04335

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  • The expression of LIM-homeobox genes, Lhx1 and Lhx5, in the forebrain is essential for neural retina differentiation

    Junji Inoue, Yuuki Ueda, Tetsuya Bando, Taro Mito, Sumihare Noji, Hideyo Ohuchi

    DEVELOPMENT GROWTH & DIFFERENTIATION   55 ( 7 )   668 - 675   2013.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/dgd.12074

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  • Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases

    Takahito Watanabe, Hiroshi Ochiai, Tetsushi Sakuma, Hadley W. Horch, Naoya Hamaguchi, Taro Nakamura, Tetsuya Bando, Hideyo Ohuchi, Takashi Yamamoto, Sumihare Noji, Taro Mito

    NATURE COMMUNICATIONS   3   2012.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/ncomms2020

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  • Expression profiles and unc-27 mutation rescue of the striated muscle type troponin I isoform-3 in Caenorhabditis elegans

    Yasuo Takashima, Shun Kitaoka, Tetsuya Bando, Hiroaki Kagawa

    GENES & GENETIC SYSTEMS   87 ( 4 )   243 - 251   2012.8

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    DOI： 10.1266/ggs.87.243

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  • Functional analysis of the role of eyes absent and sine oculis in the developing eye of the cricket Gryllus bimaculatus

    Akira Takagi, Kazuki Kurita, Taiki Terasawa, Taro Nakamura, Tetsuya Bando, Yoshiyuki Moriyama, Taro Mito, Sumihare Noji, Hideyo Ohuchi

    DEVELOPMENT GROWTH & DIFFERENTIATION   54 ( 2 )   227 - 240   2012.2

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    DOI： 10.1111/j.1440-169X.2011.01325.x

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  • Cricket body size is altered by systemic RNAi against insulin signaling components and epidermal growth factor receptor Reviewed

    Noha Dabour, Tetsuya Bando, Taro Nakamura, Katsuyuki Miyawaki, Taro Mito, Hideyo Ohuchi, Sumihare Noji

    DEVELOPMENT GROWTH & DIFFERENTIATION   53 ( 7 )   857 - 869   2011.9

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    DOI： 10.1111/j.1440-169X.2011.01291.x

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  • Regulation of Leg Size and Shape: Involvement of the Dachsous-Fat Signaling Pathway Reviewed

    Tetsuya Bando, Taro Mito, Taro Nakamura, Hideyo Ohuchi, Sumihare Noji

    DEVELOPMENTAL DYNAMICS   240 ( 5 )   1028 - 1041   2011.5

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  • The advent of RNA interference in Entomology

    Taro Mito, Taro Nakamura, Tetsuya Bando, Hideyo Ohuchi, Sumihare Noji

    ENTOMOLOGICAL SCIENCE   14 ( 1 )   1 - 8   2011.1

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  • Imaging of Transgenic Cricket Embryos Reveals Cell Movements Consistent with a Syncytial Patterning Mechanism Reviewed

    Taro Nakamura, Masato Yoshizaki, Shotaro Ogawa, Haruko Okamoto, Yohei Shinmyo, Tetsuya Bando, Hideyo Ohuchi, Sumihare Noji, Taro Mito

    CURRENT BIOLOGY   20 ( 18 )   1641 - 1647   2010.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.cub.2010.07.044

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  • Involvement of the Hedgehog/Engrailed signaling in regeneration of the posterior structures in the amputated leg of the cricket Gryllus bimaculatus

    Katsuyuki Miyawaki, Taro Mito, Taro Nakamura, Tetsuya Bando, Kimio Tanaka, Hideyo Ohuchi, Sumihare Noji

    MECHANISMS OF DEVELOPMENT   126   S294 - S294   2009.8

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  • Dynamics of cellular aggregation and differentiation during early development of transgenic crickets

    Taro Nakamura, Taro Mito, Masato Yoshizaki, Tetsuya Bando, Kimio Tanaka, Hideyo Ohuchi, Sumihare Noji

    MECHANISMS OF DEVELOPMENT   126   S254 - S254   2009.8

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  • Regulation of leg size and shape by the Dachsous/Fat signalling pathway during regeneration in Gryllus bimaculatus

    Tetsuya Bando, Taro Mito, Yuko Maeda, Taro Nakamura, Fumiaki Ito, Takahito Watanabe, Hideyo Ohuchi, Sumihare Noji

    MECHANISMS OF DEVELOPMENT   126   S293 - S294   2009.8

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  • Tissue-specific interactions of TNI isoforms with other TN subunits and tropomyosins in C. elegans: the role of the C- and N-terminal extensions. Reviewed

    Amin MZ, Bando T, Ruksana R, Anokye-Danso F, Takashima Y, Sakube Y, Kagawa H

    Biochim Biophys Acta   1774   456 - 465   2007.4

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  • A novel non-coding DNA family in Caenorhabditis elegans Reviewed

    Yasuo Takashima, Tetsuya Bando, Hiroaki Kagawa

    GENE   388 ( 1-2 )   61 - 73   2007.2

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    DOI： 10.1016/j.gene.2006.10.002

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  • Involvement of canonical Wnt/Wingless signaling in the determination of the positional values within the leg segment of the cricket Gryllus bimaculatus Reviewed

    Taro Nakamura, Taro Mito, Yoshihisa Tanaka, Tetsuya Bando, Hideyo Ohuchi, Sumihare Noji

    DEVELOPMENT GROWTH & DIFFERENTIATION   49 ( 2 )   79 - 88   2007.2

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    DOI： 10.1111/j.1440-169x.2007.00915.x

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  • Tissue expression of four troponin I genes and their molecular interactions with two troponin C isoforms in Caenorhabditis elegans. Reviewed

    Ruksana R, Kuroda K, Terami H, Bando T, Kitaoka S, Takaya T, Sakube Y, Kagawa H

    Genes Cells   10 ( 3 )   261-276 - 276   2005

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Books

  • 最先端コオロギ学 : 世界初!新しい生物学がここにある

    野地, 澄晴（ Role： Contributor ,  第6章 コオロギの切断された脚の再生メカニズム）

    北隆館  2022.4  （ ISBN:9784832610125 ）

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    Total pages：vi, 242p   Language：Japanese

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  • The Cricket as a Model Organism: Development, Regeneration, and Behavior.

    （ Role： Contributor ,  Leg formation and regeneration）

    2017.1 

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  • The Cricket as a Model Organism: Development, Regeneration, and Behavior.

    （ Role： Contributor ,  Eye development and photoreception of a hemimetabolous insect, gryllus bimaculatus）

    2017.1 

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  • クロマチンと遺伝子機能制御

    板東哲哉, 香川弘昭（ Role： Contributor ,  線虫のクロマチンと遺伝子制御）

    シュプリンガー・フェアラーク東京  2003.6 

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MISC

  • Toll receptor expressing macrophages promote cricket leg regeneration

    Tetsuya Bando, Misa Okumura, Yoshimasa Hamada, Hideyo Ohuchi

    THE NATURE & INSECTS   57 ( 1 )   33 - 36   2021.12

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    Authorship：Lead author   Language：Japanese  

    J-GLOBAL

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  • 昆虫に学ぶ器官再生

    板東哲哉, 濱田良真, 奥村美紗, 坂東優希, 大内淑代

    ケミカルタイムズ   241 ( 3 )   2 - 6   2016

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  • コオロギ脚再生の分子メカニズム

    実験医学   32 ( 1 )   15 - 21   2014

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  • Geography and history of genome: biological function of bidirectional promoter and Non-coding region in C. elegans Reviewed

    Kagawa H, Bando T, Takashima Y

    Seibutsu Butsuri   49 ( 5 )   246 - 248   2009

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    Language：Japanese   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.49.246

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    Other Link： https://jlc.jst.go.jp/DN/JALC/00337211582?from=CiNii

  • Regulation of blastema cell proliferation during cricket leg regeneration via reactive oxygen species

    奥村美紗, 板東哲哉, 大内淑代

    日本分子生物学会年会プログラム・要旨集(Web)   45th   2022

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  • Toll受容体と活性酸素による昆虫の器官再生の制御

    板東哲哉, 奥村美紗, 大内淑代

    日本解剖学会総会・全国学術集会抄録集(CD-ROM)   127th   2022

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  • Dkk3(REIC)ノックアウトマウス副腎の形態学的解析

    土生田宗憲, 藤田洋史, 佐藤恵太, 板東哲哉, 公文裕巳, 大内淑代

    日本解剖学会総会・全国学術集会抄録集(CD-ROM)   127th   2022

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  • Fgf10ゲノム編集モザイク変異マウスの肺と四肢の表現型解析

    土生田宗憲, 泰江章博, 鈴木賢一, 鈴木賢一, 藤田洋史, 佐藤恵太, 河野仁美, 高山鮎子, 板東哲哉, 宮石智, 親泊政一, 田中栄二, 大内淑代

    日本分子生物学会年会プログラム・要旨集(Web)   43rd   2020

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  • Toll受容体によるコオロギ脚再生の促進メカニズム

    奥村美紗, 板東哲哉, 坂東優希, 萩原万優, 大内淑代

    日本分子生物学会年会プログラム・要旨集(Web)   42nd   2019

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  • 紫外線受容視物質オプシン5のニワトリ胚線維芽細胞における作用

    加藤睦子, 加藤睦子, 佐藤恵太, 藤田洋史, 板東哲哉, 森實祐基, 中山正, 白神史雄, 大内淑代

    日本眼科学会雑誌   122   2018

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  • Molecular and cellular basis of regeneration and tissue repair: Dissecting insect leg regeneration through RNA interference

    T. Nakamura, T. Mito, T. Bando, H. Ohuchi, S. Noji

    Cellular and Molecular Life Sciences   65 ( 1 )   64 - 72   2008.1

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    DOI： 10.1007/s00018-007-7432-0

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  • Gene functional analyses of the cricket, Gryllus bimaculatus, using RNAi

    NAKAMURA Taro, HAMADA Asuka, BANDO Tetsuya, MITO Taro, OHUCHI Hideyo, NOJI Sumihare

    IEICE technical report   107 ( 154 )   91 - 93   2007.7

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    A cricket Gryllus bimaculatus is a typical hemimetabolous intermediate germ insect, in which the processes of segmentation and appendage formation differ considerably from those in Drosophila, a holometabolous long germ insect. Furthermore, the nymph of cricket possesses the ability to regenerate these legs. To gain insight into molecular mechanisms of development and leg regeneration, we established the embryonic, parental and nymphal RNAi techniques to analyze their functions. Our RNAi analyses revealed that (1) Caudal probably acts as a crucial morphogen with a posterior-to-anterior gradient to form of posterior region; (2) the Wingless signaling pathway is involved in the process of leg regeneration and determination of positional information in the leg segment; (3) Fragile X, Gryllus ortholog of responsible gene for human fragile x syndrome, is involved in the cricket calling song; (4) Laccase 2 coding for diphenol oxidase is involved in cuticle tanning (or sclerotization and pigmentation) in the cricket.

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  • Tissue expression of the tropomyosin gene of Caenorhabditis elegans--enhancer modification of the promoter control

    Hiroaki Kagawa, Tetsuya Bando

    Seikagaku. The Journal of Japanese Biochemical Society   74 ( 9 )   1176 - 1180   2002

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Presentations

  • コオロギ脚再生においてDual oxidaseが産生する活性酸素は細胞増殖と血球遊走を制御する

    板東 哲哉, 奥村 美紗, 大内 淑代

    第47回日本分子生物学会年会  2024.11.29 

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    Event date： 2024.11.27 - 2024.11.29

    Language：Japanese   Presentation type：Poster presentation  

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  • Leg regeneration and Innate immunity in Gryllus bimaculatus Invited

    Tetsuya Bando, Misa Okumura, Hideyo Ohuchi

    Looking back of studies on planarian regeneration and future directions  2024.8.21 

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    Event date： 2024.8.20 - 2024.8.21

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

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  • Regulation of blastema cell proliferation during cricket leg regeneration via reactive oxygen species

    Misa Okumura, Tetsuya Bando, Hideyo Ohuchi

    The 56th annual meeting of the Japanese Society of Developmental Biologists  2023.7.23 

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    Event date： 2023.7.22 - 2023.7.25

    Language：English   Presentation type：Poster presentation  

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  • Regulation of blastema cell proliferation during cricket leg regeneration via reactive oxygen species

    Misa Okumura, Tetsuya Bando, Hideyo Ohuchi

    2022.11.30 

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    Event date： 2022.11.30 - 2022.12.2

    Presentation type：Poster presentation  

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  • マクロファージと活性酸素による昆虫脚再生の制御

    板東哲哉、奥村美紗、大内淑代

    日本解剖学会 第 76 回 中国・四国支部学術集会  2022.10.30 

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    Event date： 2022.10.29 - 2022.10.30

    Presentation type：Oral presentation (general)  

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  • Regulation of blastema cell proliferation during cricket leg regeneration via NADPH oxidases

    Tetsuya Bando, Misa Okumura, Hideyo Ohuchi

    2021.12.2 

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    Event date： 2021.12.1 - 2021.12.3

    Presentation type：Poster presentation  

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  • Toll-like receptor and scavenger receptor CD36 promote blastema cell proliferation during insect leg regeneration via macrophages

    Tetsuya Bando, Misa Okumura, Hideyo Ohuchi

    2021.3.29 

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    Event date： 2021.3.28 - 2021.3.30

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  • Toll様受容体とスカベンジャー受容体CD36による昆虫の器官再生メカニズム

    板東哲哉, 奥村美紗, 坂東優希, 萩原万優, 大内淑代

    第125回日本解剖学会総会・全国学術集会  2020.3.26 

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    Event date： 2020.3.25 - 2020.3.27

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  • Fgf10モザイク変異体における肺表現型および下肢帯表現型の形態学的解析

    土生田宗憲, 泰江章博, 鈴木賢一, 高山鮎子, 藤田洋史, 板東哲哉, 佐藤恵太, 親泊政一, 田中栄二, 大内淑代

    日本先天異常学会学術集会プログラム・抄録集  2020 

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  • ゲノム編集Fgf10モザイク変異体の遺伝子型と表現型解析

    土生田宗憲, 泰江章博, 鈴木賢一, 藤田洋史, 高山鮎子, 佐藤恵太, 板東哲哉, 親泊政一, 田中栄二, 大内淑代

    日本解剖学会総会・全国学術集会講演プログラム・抄録集  2020 

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  • Toll-like receptors promote leg regeneration in the cricket

    2019.12.3 

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    Event date： 2019.12.3 - 2019.12.6

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  • 神経網膜形成における転写因子Lhx1の下流遺伝子の機能解析

    衣畑俊希, 佐藤恵太, 藤田洋史, 板東哲哉, 濃野勉, 大内淑代

    日本分子生物学会年会プログラム・要旨集(Web)  2019 

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  • REIC/Dickkopf3(Dkk3)の副腎における機能解析

    土生田宗憲, 藤田洋史, 佐藤恵太, 板東哲哉, 公文裕巳, 大内淑代

    日本解剖学会総会・全国学術集会講演プログラム・抄録集  2019 

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  • 極性分子複合体はコオロギ脚再生に必要である

    板東 哲哉, 濱田 良真, 三戸 太郎, 野地 澄晴, 大内 淑代

    第41回日本分子生物学会年会  2018.11.29 

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    Event date： 2018.11.28 - 2018.11.30

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  • マクロファージによる器官再生の促進:昆虫の脚再生をモデルとして

    板東哲哉, 奥村美紗, 坂東優希, 萩原万優子, 濱田良真, 大内淑代

    日本解剖学会 第73回中国・四国支部学術集会  2018.10.20 

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    Event date： 2018.10.20 - 2018.10.21

    Presentation type：Oral presentation (general)  

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  • ゲノム編集Fgf10モザイク変異体の組織学的解析

    土生田宗憲, 泰江章博, 藤田洋史, 板東哲哉, 佐藤恵太, 親泊政一, 田中栄二, 大内淑代

    日本解剖学会総会・全国学術集会講演プログラム・抄録集  2018 

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  • Fgf10モザイク変異体における肺表現型の形態学的解析

    土生田宗憲, 泰江章博, 藤田洋史, 板東哲哉, 佐藤恵太, 親泊政一, 田中栄二, 大内淑代

    日本先天異常学会学術集会プログラム・抄録集  2018 

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  • Lhx1過剰発現による二重網膜誘導モデルを用いた網膜初期発生の研究

    衣畑俊希, 佐藤恵太, 藤田洋史, 板東哲哉, 大内淑代

    日本分子生物学会年会プログラム・要旨集(Web)  2018 

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  • CRISPR/Cas9システムにより作製したモザイク変異マウスの組織学的解析

    土生田宗憲, 泰江章博, 藤田洋史, 板東哲哉, 佐藤恵太, 親泊政一, 田中栄二, 大内淑代

    日本生化学会大会(Web)  2017 

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  • Fat and dachsous protocadherin are required for proper proximodistal and circumferential regeneration of amputated legs in the cricket, Gryllus bimaculatus

    Tetsuya Bando, Yuko Maeda, Taro Nakamura, Taro Mito, Hideyo Ohuchi, Sumihare Noji

    GENES & GENETIC SYSTEMS  2007.12  GENETICS SOC JAPAN

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    Event date： 2007.12

    Language：English  

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  • Warts, Expanded and Dachs are required for proper regeneration of amputated legs in the cricket, Gryllus bimaculatus

    Yuko Maeda, Tetsuya Bando, Taro Nakamura, Taro Mito, Hideyo Ohuchi, Sumihare Noji

    GENES & GENETIC SYSTEMS  2007.12  GENETICS SOC JAPAN

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    Event date： 2007.12

    Language：English  

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  • Innate immunity signaling pathways promote leg regeneration in the cricket

    第70回日本細胞生物学会 第51回日本発生生物学会 合同大会  2018 

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  • 再生モデル昆虫ではToll様受容体が器官再生を促進する

    第123回 日本解剖学会総会・全国学術集会  2018 

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  • 再生モデル昆虫における自然免疫シグナル経路を介した器官再生の促進メカニズム

    ConBio2017  2017 

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  • 再生モデル昆虫におけるマクロファージを介した再生開始メカニズム

    第122回 日本解剖学会総会・全国学術集会  2017 

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  • がん関連遺伝子 Dickkopf-3（Dkk-3）の生体内における発現パターンの解析

    第57回日本組織細胞化学会総会・学術集会  2016 

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  • 再生モデル昆虫における器官再生のメカニズム

    第121回 日本解剖学会総会・全国学術集会  2016 

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  • フタホシコオロギ脚再生におけるマクロファージを中心とした炎症反応の役割

    第2回 ユニークな少数派実験動物を扱う若手が最先端アプローチを勉強する会  2016 

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  • Molecular link between innate immunity and tissue regeneration

    Cricket Meeting 2016  2016 

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  • Angiomotin regulates cell proliferation cooperatively with Expanded and Merlin during cricket leg regeneration

    第38回 日本分子生物学会年会  2015 

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  • Cyclin B3 is involved in leg regeneration of the cricket Gryllus bimaculatus

    第120回 日本解剖学会総会・全国学術集会  2015 

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  • Epigenetic regulation on histone H3K27 is involved in redifferentiation process during leg regeneration in the cricket Gryllus bimaculatus

    第120回 日本解剖学会総会・全国学術集会  2015 

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  • ポリプテルス眼の発生分化と機能に関わる遺伝子群

    第５回 Tokyo Vertebrate Morphology Meeting  2015 

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  • 再生モデル昆虫コオロギに学ぶ器官再生のメカニズム

    再生生物学シンポジウム『次世代型器官再生生物学の発展』  2015 

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  • コオロギ脚再生において再生芽細胞の再分化はヒストンH3K27メチル化により制御される

    第37回 日本分子生物学会年会  2014 

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  • CRISPR/CasシステムによるPax6遺伝子破壊マウスの解析

    第37回 日本分子生物学会年会  2014 

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  • コオロギにおけるエピジェネティック因子E(z)の概日リズムおよび再生への関与

    日本動物学会 第85回大会  2014 

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  • コオロギに学ぶ器官再生の分子メカニズム

    第１１９回 日本解剖学会総会・全国学術集会  2014 

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  • Molecular basis on tissue regeneration in the cricket as revealed by RNA interference

    International Symposium on RNAi and Genome Editing Research  2014 

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  • Mechanisms underlying regeneration of the cricket leg, based on an extended steepness model

    EMBO Conference The Molecular and cellular basis of regeneration and tissue repair  2014 

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  • Epigenetic regulation of genes expressions via methylation on histone H3 27th lysine residue during leg regeneration

    第47回 日本発生生物学会  2014 

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  • Epigenetic regulation of genes expressions via methylation on histone H3K27 during leg regeneration in the cricket Gryllus bimaculatus

    CDBシンポジウム2014 Regeneration of Organs: Programming and Self-Organization  2014 

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  • 再生モデル昆虫を用いた器官再生の分子基盤の研究

    第12回日本再生医療学会総会  2013 

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  • 再生を制御する普遍的分子機構の解明：昆虫の脚再生からヒト器官再生を目指して

    日本解剖学会 第68回中国・四国支部学術集会  2013 

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  • フタホシコオロギ脚再生過程におけるパターン形成遺伝子のエピジェネティックな発現制御

    日本動物学会第84回大会  2013 

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  • 器官再生において再生芽細胞の再分化を制御するエピジェネティックな機構

    日本分子生物学会第36回年会  2013 

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  • JAK/STAT signaling promotes blastemal cell proliferation during leg regeneration in the cricket Gryllus bimaculatus

    第46回日本発生生物学会  2013 

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  • コオロギ脚再生において再生芽細胞の増殖を制御する分子メカニズムの解析

    第35回日本分子生物学会年会  2012 

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  • Molecular mechanism for regulation of blastema cell proliferation during leg regeneration in Gryllus bimaculatus

    2012 

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Research Projects

  • Toll様受容体シグナルが促進する脱分化誘導因子のエピジェネティックな発現制御

    Grant number：21K06121  2021.04 - 2024.03

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    板東　哲哉

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\4160000 （ Direct expense: \3200000 、 Indirect expense：\960000 ）

  • マクロファージにより活性化される脱分化誘導因子のエピジェネティックな発現制御

    Grant number：18K06184  2018.04 - 2022.03

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    板東　哲哉

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\4290000 （ Direct expense: \3300000 、 Indirect expense：\990000 ）

  • 器官再生において再生芽細胞への脱分化を誘導するエピジェネティックな機構

    Grant number：15K06897  2015.04 - 2018.03

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    板東　哲哉

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\5070000 （ Direct expense: \3900000 、 Indirect expense：\1170000 ）

  • 器官再生において再生芽細胞の脱分化と再分化を制御するエピジェネティックな機構

    Grant number：25830133  2013.04 - 2015.03

    日本学術振興会  科学研究費助成事業  若手研究(B)

    板東　哲哉

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\4160000 （ Direct expense: \3200000 、 Indirect expense：\960000 ）

  • 器官再生における遺伝子発現のエピジェネティックな制御

    Grant number：23710217  2011.04 - 2013.03

    日本学術振興会  科学研究費助成事業  若手研究(B)

    板東　哲哉

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\4550000 （ Direct expense: \3500000 、 Indirect expense：\1050000 ）

  • Ｄａｃｈｓｏｕｓ／Ｆａｔシグナリング系を介した位置情報決定メカニズムの解明

    Grant number：22124003  2010.04 - 2015.03

    日本学術振興会  科学研究費助成事業  新学術領域研究(研究領域提案型)

    野地 澄晴,大内 淑代,三戸 太郎,板東 哲哉

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    Authorship：Coinvestigator(s)  Grant type：Competitive

    Grant amount：\98670000 （ Direct expense: \75900000 、 Indirect expense：\22770000 ）

  • 脳の”眼”を構成するオプシン５視細胞の機能解明とその発生分化メカニズム

    Grant number：24500382  2012.04 - 2015.03

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    大内 淑代,板東 哲哉,藤田 洋史,泰江 章博,井上 順治,加藤 睦子

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    Authorship：Coinvestigator(s)  Grant type：Competitive

    Grant amount：\5200000 （ Direct expense: \4000000 、 Indirect expense：\1200000 ）

  • 器官再生において活性酸素が制御する未分化維持因子の発現と再生芽細胞への脱分化

    Grant number：24K09412  2024.04 - 2027.03

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    板東 哲哉

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    Grant amount：\4550000 （ Direct expense: \3500000 、 Indirect expense：\1050000 ）

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  • Toll様受容体シグナルが促進する脱分化誘導因子のエピジェネティックな発現制御

    Grant number：21K06121  2021.04 - 2024.03

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    板東 哲哉

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    Grant amount：\4160000 （ Direct expense: \3200000 、 Indirect expense：\960000 ）

    フタホシコオロギの後脚を脛節で切断すると、創傷部位がかさぶたに覆われ、創傷部周辺にToll受容体を発現するプラズマ細胞（昆虫のマクロファージ）が遊走する。プラズマ細胞はサイトカインを産生して周辺の細胞分裂を増加させ、再生芽の形成を促進する。傷口へのプラズマ細胞の遊走は、微生物等の感染ではなく、障害された細胞由来のDAMPsがきっかけであった（Bando et al., Development, 2021 (in press）、板東ら, 昆虫と自然, 2021）。
    再生時におけるプラズマ細胞の機能をより詳細に解析するため、プラズマ細胞から産生される活性酸素種(ROS)に着目した。細胞外にROSを産生するNADPH oxidasesは、コオロギではNox5とDuoxの2遺伝子にコードされている。Nox5(RNAi)個体は正常に再生したが、Duox(RNAi)個体では再生芽の肥大や幼虫成長の不全が観察された。Duox maturation factor (DuoxA)に対するRNAi個体でも再生能の低下が観察されたことから、ROSの産生は再生芽細胞の増殖制御に関わると考えられた。しかしながらNADPH oxidases阻害剤処理したコオロギでは再生芽の肥大は観察されず、再生能が低下する表現型が観察された。そこでROSに応答する転写因子複合体Keap1/Nrf2の再生過程における機能を調べた。Keap1(RNAi)個体では再生能の低下が見られたが、Nrf2(RNAi)個体は脱皮不全を起こして再生過程への影響は不明であった。Nrf2の標的遺伝子の1つcatalaseに対するRNAiを行った場合は再生能が低下したため、ROSの消去が低下して高ROS状態になっても再生能が低下すると考えられた。

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  • Epigenetic regulation of dedifferentiation-inducible genes mediated by macrophage during regeneration

    Grant number：18K06184  2018.04 - 2022.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Bando Tetsuya

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    Grant amount：\4290000 （ Direct expense: \3300000 、 Indirect expense：\990000 ）

    The two-spotted cricket Gryllus bimaculatus regenerates the lost part of leg. During the leg regeneration process, the blastema, which is the population of undifferentiated-proliferating cells, is formed beneath the wound epithelium. The blastema cells proliferate and differentiate to regenerate the lost part of leg, however, molecular mechanisms of the blastema formation are unclear. In this study, we found that the plasmatocytes, which are known as insect macrophages, recognized debris of injured cells and accumulated into the wound site. The plasmatocytes produced the insect cytokine Upd to activate Jak/STAT signaling, inducing the blastema cell proliferation and leg regeneration.

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  • Epigenetic regulation for blastema formation during leg regeneration in the cricket

    Grant number：15K06897  2015.04 - 2018.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Bando Tetsuya, MITO TARO

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    Grant amount：\5070000 （ Direct expense: \3900000 、 Indirect expense：\1170000 ）

    When we amputate a leg of the two-spotted cricket, cricket regenerates lost part of leg from blastema cells, which are multipotent-proliferating cells. Blastema cells are thought as dedifferentiated cells from differentiated cells mediated by epigenetic changes of gene expression, however, detail mechanism of the epigenetic regulation during blastema formation is unclear. E(z) and Utx methyltransferas and demethylates at histone H3K27. These enzymes regulated repatterning process but did not regulate dedifferentiation process. We found that macrophages were essential for leg regeneration in the cricket via the activation of Jak/STAT and TLR signaling pathways. In the macrophage-depleted regenerating leg, histone H3K9 acetylation was decreased, maybe via ROS production by Crq/CD36 and NADPH oxidase. Cbp and Rpd3, which encode histone acetyltransferase and histone deacetylase, respectively, were needed for leg regeneration.

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  • Epigenetic regulation of dedifferentiation and redifferentiation of blastema cells during tissue regeneration

    Grant number：25830133  2013.04 - 2015.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (B)

    BANDO Tetsuya

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    Grant amount：\4160000 （ Direct expense: \3200000 、 Indirect expense：\960000 ）

    Hemimetabolous insect, cricket regenerate lost tissue parts using blastemal cells, which is a population of dedifferentiated-proliferating cells. Gene expression of epigenetic factors is upregulated in the blastema compared with in differentiated tissue, suggesting that epigenetic changes in gene expression may control the differentiation status of blastema cells. We focused on the function of the E(z) and Utx that regulate the methylation and demethylation on histone H3 27th lysine residue, respectively. Regenerated E(z)RNAi cricket legs exhibited extra tibia segment formation between the tibia and tarsus, and regenerated UtxRNAi cricket legs showed leg joint formation defects in the tarsus. In the E(z)RNAi- and UtxRNAi-regenerating leg, the dac and Egfr expressions were abnormal. These results suggest that regulation of the histone H3K27 methylation state is involved in the repatterning process during leg regeneration via the epigenetic regulation of leg patterning gene expression.

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  • Epigenetic regulation of geneexpressions via methylation of histone tail during tissue regeneration

    Grant number：23710217  2011 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (B)

    BANDO Tetsuya

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    Grant amount：\4550000 （ Direct expense: \3500000 、 Indirect expense：\1050000 ）

    Hemimetabolous insects, such as cricket Gryllus bimaculatus, have remarkable regenerative capacity. When cricket nymph loses a part of leg, the lost part of leg is regenerated from blastema, which is a population of dedifferentiated proliferating cells. In the blastema cells, expressions of several epigenetic protein genes including Enhancer of zeste(E(z)), which encodes methyltransferase on histone H3 27th lysine residue (H3K27), and Utx, which encodes demethylase for methylated histone H3K27, were upregulated compared with differentiated cells. We performed RNAi against E(z)a n d Utx. Morphologies of regeneration legs of E(z)(RNAi) and Utx(RNAi) cricket nymphs were abnormal. Several leg patterning genes were ectopically expressed in regeneration leg of E(z)(RNAi) cricket and were diminished in Utx(RNAi) cricket. Methylation of histone H3K27 was diminished and increased in E(z)(RNAi) and Utx(RNAi) crickets, respectively, suggesting that epigenetic regulation of leg patterning genes expressions via methylation of histone H3K27 is involved in repatterning process during leg regeneration.

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  • Mechanisms underlying determination of positional information through Dachsous/Fat signaling system

    Grant number：22124003  2010.04 - 2015.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    NOJI Sumihare, OHUCHI Hideyo, MITO Taro, BANDO Tetsuya

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    Grant amount：\98670000 （ Direct expense: \75900000 、 Indirect expense：\22770000 ）

    We have investigated molecular mechanisms underlying insect leg regeneration by means of regeneration-dependent RNA interference. Our results suggested that leg regeneration occurs as follows:
    (1) Activation of macrophage-like cells by amputation at the amputated site, (2) Cytokines produced by the macrophage-like cells induce formation of a blastema consisting of undifferentiated cells, (3) In the blastema, EGF (epidermal growth factor) is induced by cross-talk of Wnt/BMP signaling pathways. Furthermore, genes involved in epigenetic regulations such as Enhancer of Zest and Utx are activated, (4) EGF induces expressions of dachshund/Distal-less and regulates expression of Dachsous/Fat signaling systems, which then determine re-patterning of the leg and its size. We speculated that the leg size regulation may be related to the number of Dachsous/Fat molecules in each cell membrane with a proximodistal gradient. This was simulated with our modified steepness model.

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  • Studies on an ultraviolet-absorbing photopigment, Opsin 5 in vertebrate eye and brain

    Grant number：24500382  2012.04 - 2015.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    OHUCHI Hideyo, BANDO Tetsuya, FUJITA Hirofumi, YASUE Akihiro, INOUE Junji, KATO Mutsuko

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    Grant amount：\5200000 （ Direct expense: \4000000 、 Indirect expense：\1200000 ）

    This research project was aimed for clarifying the functions for an ultraviolet-absorbing photopigment, Opsin 5 in vertebrates such as mouse and chicken by use of a genome-editing technique, TALEN (Transcription activator-like effector nuclease). We succeeded in production of Opn5-knockout mice by this method, finding that Opn5-deficient mice were born alive and seemed healthy at least after 3 weeks postnatal.

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