Updated on 2025/07/02

写真a

 
MARUYAMA Masato
 
Organization
Faculty of Medicine, Dentistry and Pharmaceutical Sciences Associate Professor
Position
Associate Professor
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Degree

  • PhD ( Kyoto University )

Research Areas

  • Life Science / Clinical pharmacy

Professional Memberships

  • 日本薬剤学会 日本薬物動態学会 日本薬学会 日本癌学会

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Papers

  • Repeated sequential administration of pegylated emulsion of SU5416 and liposomal paclitaxel enhances anti-tumor effect in 4T1 breast cancer-bearing mice

    Masato Maruyama, Reiya Torii, Hazuki Matsui, Hiroki Hayashi, Ken-ichi Ogawara, Kazutaka Higaki

    European Journal of Pharmaceutics and Biopharmaceutics   209   114663 - 114663   2025.4

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    Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.ejpb.2025.114663

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  • Dynamic changes in the distribution equilibrium of drugs in microemulsions associated with drug absorption facilitate the absorption improvement for drugs with low water-solubility by self-microemulsifying drug delivery system (SMEDDS)

    Saki Nishiyama, Yuki Takemoto, Keita Yamanouchi, Keiji Kondo, Sho Kawatsu, Masato Maruyama, Kazutaka Higaki

    International Journal of Pharmaceutics   125458 - 125458   2025.3

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    Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.ijpharm.2025.125458

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  • EGF-induced P-gp expression in tumor vasculature contributes to therapeutic resistance to Doxorubicin-PEG-liposomes in mice bearing Doxorubicin-resistant B16-BL6 tumors

    Masato Maruyama, Tomoki Ueda, Yusuke Ienaka, Haruka Tojo, Kenji Hyodo, Ken-ichi Ogawara, Kazutaka Higaki

    Journal of Drug Delivery Science and Technology   106690 - 106690   2025.2

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    Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.jddst.2025.106690

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  • Specific intermolecular interaction with sodium glycocholate generates the co-amorphous system showing higher physical stability and aqueous solubility of Y5 receptor antagonist of neuropeptide Y, a brick dust molecule Reviewed

    Shohei Aikawa, Hironori Tanaka, Hiroshi Ueda, Masato Maruyama, Kazutaka Higaki

    European Journal of Pharmaceutics and Biopharmaceutics   202   114395 - 114395   2024.9

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.ejpb.2024.114395

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  • Vascular normalization with pegylated emulsion of SU5416 enhances anti-tumor effect of liposomal paclitaxel in 4T1 breast cancer-bearing mice: Analysis of intratumoral vessels and microenvironment Reviewed

    Masato Maruyama, Hazuki Matsui, Haruka Nakamura, Reiya Torii, Yuta Takasugi, Ken-ichi Ogawara, Kazutaka Higaki

    Journal of Drug Delivery Science and Technology   96   105647   2024.6

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

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  • Possible Regulation of P-glycoprotein Function by Adrenergic Agonists II: Study with isolated rat jejunal sheets and Caco-2 cell monolayers Reviewed International journal

    Hironori Mukai, Masashi Takanashi, Ken-ichi Ogawara, Masato Maruyama, Kazutaka Higaki

    Journal of Pharmaceutical Sciences   113 ( 5 )   1209 - 1219   2024.5

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    To clarify the regulation of drug absorption by the enteric nervous system, we investigated how adrenergic agonists (adrenaline (ADR), clonidine (CLO), dobutamine (DOB)) and dibutyryl cAMP (DBcAMP) affected P-glycoprotein (P-gp) function by utilizing isolated rat jejunal sheets and Caco-2 cell monolayers. ADR and CLO significantly decreased the secretory transport (Papptotal) of rhodamine-123 and tended to decrease the transport via P-gp (PappP-gp) and passive transport (Papppassive). In contrast, DBcAMP significantly increased and DOB tended to increase Papptotal and both tended to increase PappP-gpand Papppassive. Changes in P-gp expression on brush border membrane by adrenergic agonists and DBcAMP were significantly correlated with PappP-gp, while P-gp expression was not changed in whole cell homogenates, suggesting that the trafficking of P-gp would be responsible for its functional changes. Papppassive was inversely correlated with transmucosal or transepithelial electrical resistance, indicating that adrenergic agonists affected the paracellular permeability. Adrenergic agonists also changed cAMP levels, which were significantly correlated with PappP-gp. Furthermore, protein kinase A (PKA) or PKC inhibitor significantly decreased PappP-gp in Caco-2 cell monolayers, suggesting that they would partly contribute to the changes in P-gp activity. In conclusion, adrenergic agonists regulated P-gp function and paracellular permeability, which would be caused via adrenoceptor stimulation.

    DOI: 10.1016/j.xphs.2023.11.010

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  • Combination of co-amorphization with SNEDDS outperforms Ofev® in the oral absorption of nintedanib Reviewed

    Tomoya Inoue, Seito Maehara, Masato Maruyama, Kazutaka Higaki

    International Journal of Pharmaceutics   657   124197 - 124197   2024.5

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    Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.ijpharm.2024.124197

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  • Development of Sustained Release System Based on High Water-Absorbable Gel Formation Using Croscarmellose Sodium, Alkaline Excipients and HPMC (ACSH SR System); Novel Application of Croscarmellose Sodium as a Gel Former Reviewed

    Masato Gomi, Naoya Mizutani, Ryotaro Senoo, Noriaki Matsubara, Ayahisa Watanabe, Masato Maruyama, Go Kimura, Kazutaka Higaki

    Pharmaceutical Research   2023.11

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s11095-023-03630-w

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    Other Link: https://link.springer.com/article/10.1007/s11095-023-03630-w/fulltext.html

  • Improvement and characterization of oral absorption behavior of clofazimine by SNEDDS: Quantitative evaluation of extensive lymphatic transport Reviewed

    Keita Yamanouchi, Tomoki Ishimaru, Takuya Kakuno, Yuki Takemoto, Sho Kawatsu, Keiji Kondo, Masato Maruyama, Kazutaka Higaki

    European Journal of Pharmaceutics and Biopharmaceutics   187   141 - 155   2023.6

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    Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.ejpb.2023.04.009

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  • Establishment of a novel in vitro co-culture system of enteric neurons and Caco-2 cells for evaluating the effect of enteric nervous system on transepithelial transport of drugs Reviewed

    Masato Maruyama, Minami Yoshikata, Mana Sakaguchi, Shizuka Wakushima, Kazutaka Higaki

    International Journal of Pharmaceutics   633   122617 - 122617   2023.2

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.ijpharm.2023.122617

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  • Formation of a Stable Co-Amorphous System for a Brick Dust Molecule by Utilizing Sodium Taurocholate with High Glass Transition Temperature Reviewed

    Shohei Aikawa, Hironori Tanaka, Hiroshi Ueda, Masato Maruyama, Kazutaka Higaki

    Pharmaceutics   15 ( 1 )   84 - 84   2022.12

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    Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    Brick dust molecules are usually poorly soluble in water and lipoidal components, making it difficult to formulate them in dosage forms that provide efficient pharmacological effects. A co-amorphous system is an effective strategy to resolve these issues. However, their glass transition temperatures (Tg) are relatively lower than those of polymeric amorphous solid dispersions, suggesting the instability of the co-amorphous system. This study aimed to formulate a stable co-amorphous system for brick dust molecules by utilizing sodium taurocholate (NaTC) with a higher Tg. A novel neuropeptide Y5 receptor antagonist (AntiY5R) and NaTC with Tg of 155 °C were used as the brick dust model and coformer, respectively. Ball milling formed a co-amorphous system for AntiY5R and NaTC (AntiY5R-NaTC) at various molar ratios. Deviation from the theoretical Tg value and peak shifts in Fourier-transform infrared spectroscopy indicated intermolecular interactions between AntiY5R and NaTC. AntiY5R-NaTC at equal molar ratios resulting in an 8.5-fold increase in AntiY5R solubility over its crystalline form. The co-amorphous system remained amorphous for 1 month at 25 °C and 40 °C. These results suggest that the co-amorphous system formed by utilizing NaTC as a coformer could stably maintain the amorphous state and enhance the solubility of brick dust molecules.

    DOI: 10.3390/pharmaceutics15010084

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  • Analysis of absorption-enhancing mechanisms for combinatorial use of spermine with sodium taurocholate in Caco-2 cells Reviewed

    Masato Maruyama, Yohei Nishida, Hironori Tanaka, Takako Minami, Ken-ichi Ogawara, Masateru Miyake, Yuta Takamura, Hiroki Kakuta, Kazutaka Higaki

    European Journal of Pharmaceutics and Biopharmaceutics   180   332 - 343   2022.11

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.ejpb.2022.10.020

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  • Efficient Evaluation of In Vivo Performance in Human for Generic Formulation by Novel Dissolution-Absorption Prediction (DAP) Workflow Reviewed

    Motoki Onishi, Kozo Tagawa, Maiko Jiko, Kayo Koike, Masato Maruyama, Hidetoshi Hashizume, Kazuhide Imagaki, Kazutaka Higaki

    Pharmaceutical Research   39   2203 - 2216   2022.7

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    Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s11095-022-03337-4

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    Other Link: https://link.springer.com/article/10.1007/s11095-022-03337-4/fulltext.html

  • Effect of Excessive Serotonin on Pharmacokinetics of Cephalexin after Oral Administration: Studies with Serotonin-Excessive Model Rats Reviewed

    Shun Nakashima, Takeharu Iwamoto, Masashi Takanashi, Ken-ichi Ogawara, Masato Maruyama, Kazutaka Higaki

    Pharmaceutical Research   2022.7

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    Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s11095-022-03325-8

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    Other Link: https://link.springer.com/article/10.1007/s11095-022-03325-8/fulltext.html

  • Extensive improvement of oral bioavailability of mebendazole, a brick dust, by polymer-containing SNEDDS preparation: Disruption of high crystallinity by utilizing its counter ion Reviewed

    Yusuke Sumimoto, Shinya Okawa, Tomoya Inoue, Kazufumi Masuda, Masato Maruyama, Kazutaka Higaki

    European Journal of Pharmaceutics and Biopharmaceutics   172   213 - 227   2022.3

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    Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.ejpb.2022.02.002

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  • Effect of Doxorubicin Release Rate From Polyethylene Glycol-Modified Liposome on Anti-tumor Activity in B16-BL6 Tumor-Bearing Mice Reviewed

    Masato Maruyama, Haruka Tojo, Keita Toi, Yusuke Ienaka, Kenji Hyodo, Hiroshi Kikuchi, Ken-ichi Ogawara, Kazutaka Higaki

    Journal of Pharmaceutical Sciences   111 ( 2 )   293 - 297   2022.2

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.xphs.2021.11.020

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  • Sequential administration of PEG-Span 80 niosome enhances anti-tumor effect of doxorubicin-containing PEG liposome Reviewed

    Takaya Minamisakamoto, Shuhei Nishiguchi, Kazuki Hashimoto, Ken-ichi Ogawara, Masato Maruyama, Kazutaka Higaki

    European Journal of Pharmaceutics and Biopharmaceutics   169   20 - 28   2021.12

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    Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.ejpb.2021.08.013

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  • Spermine with Sodium Taurocholate Enhances Pulmonary Absorption of Macromolecules in Rats. Reviewed International journal

    Masateru Miyake, Takanori Minami, Masato Maruyama, Tadashi Mukai, Kazutaka Higaki

    Journal of pharmaceutical sciences   110 ( 10 )   3464 - 3470   2021.10

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    The improvement effect of the combined use of spermine (SPM), a polyamine, with sodium taurocholate (STC) on the pulmonary drug absorption was investigated utilizing poorly absorbable drugs with various molecular sizes in rats. The pulmonary absorption of rebamipide, a low molecular but poorly absorbable drug after oral administration, was significantly improved by the combined use of SPM with STC (SPM-STC formulation), while poly- L-lysine did not show a significant change in rebamipide absorption from the lungs. Furthermore, the safety of the SPM-STC formulation for the lungs was assessed in rats by the histopathological study and any local toxicity was not observed while poly-L-lysine, a typical chemical causing the toxicity for the epithelial cells, provided several histopathological changes. In addition, the SPM-STC formulation significantly improved the pulmonary absorption of fluorescein isothiocyanate dextran 4 (FD-4, Mw ca 4000) and interferon-α (IFN-α, Mw ca 25,000) as well. Our present results clearly indicated that the SPM-STC formulation significantly improved the pulmonary absorption of poorly absorbable small and large molecular drugs without any harmful effects on the lungs. Therefore, the SPM-STC formulation would be a useful one for the pulmonary absorption of drugs, specifically macromolecular ones, which are very difficult to be absorbed after oral administration.

    DOI: 10.1016/j.xphs.2021.06.015

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  • Possible Regulation of P-glycoprotein Function by Adrenergic Agonists in a Vascular-luminal Perfused Preparation of Small Intestine Reviewed

    Hironori Mukai, Masashi Takanashi, Ken-ichi Ogawara, Masato Maruyama, Kazutaka Higaki

    Journal of Pharmaceutical Sciences   2021.9

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    DOI: 10.1016/j.xphs.2021.09.014

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  • PC3-Secreted Microprotein Is Expressed in Glioblastoma Stem-Like Cells and Human Glioma Tissues. Reviewed

    Masato Maruyama, Yousuke Nakano, Takuya Nishimura, Ryoichi Iwata, Satoshi Matsuda, Mikio Hayashi, Yuki Nakai, Masahiro Nonaka, Tetsuo Sugimoto

    Biological & pharmaceutical bulletin   44 ( 7 )   910 - 919   2021.7

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    Glioblastoma multiforme (GBM) is the most prevalent malignant primary brain tumor with a high recurrence rate. Despite multimodal therapy including surgical resection, chemotherapy, and radiotherapy, the median survival time after the initial diagnosis of GBM is approximately 14 months. Since cancer stem cells (CSCs) are considered the leading cause of cancer recurrence, glioblastoma stem cell-targeted therapy is a promising strategy for the treatment of GBM. However, because CSC heterogeneity has been implicated in the difficulties of CSC-target therapy, more in-depth knowledge of CSC biology is still required to develop novel therapies. In this study, we established single cell-derived tumorspheres from human glioblastoma U87MG cells. One of these tumorspheres, P4E8 clone, showed CSC-like phenotypes, such as self-renewal capacity, expression of CSC markers, resistance to anti-cancer agents, and in vivo tumorigenicity. Therefore, we used P4E8 cells as a cell-based model of glioblastoma stem cells (GSCs). Gene expression analysis using microarray indicated that the most highly expressed genes in P4E8 cells compared to the parental U87MG were PC3-secreted microprotein (MSMP). Furthermore, MSMP was expressed in patient-derived GSCs and human glioma tissues at the protein level, implying that MSMP might contribute to glioma development and progression.

    DOI: 10.1248/bpb.b20-00868

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  • Improvement of lipid solubility and oral bioavailability of a poorly water- and poorly lipid-soluble drug, rebamipide, by utilizing its counter ion and SNEDDS preparation. Reviewed International journal

    Shinya Okawa, Yusuke Sumimoto, Kazufumi Masuda, Ken-Ichi Ogawara, Masato Maruyama, Kazutaka Higaki

    European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences   159   105721 - 105721   2021.1

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    Among drugs in development and/or in market, there are poorly water-soluble and poorly lipid-soluble compounds. Rebamipide, classified into BCS class IV, is one of those drugs which provide very low bioavailability and/or the difficulty of formulation for oral administration. Because of its low solubility in available lipoidal excipients, it was impossible to prepare an adequate SNEDDS formulation of rebamipide. Then, we tried to increase the solubility of rebamipide in lipoidal excipients for preparing a more practical SNEDDS formulation by making the complex with its counter ion, tetrabutylphosphonium hydroxide (TBPOH) or NaOH. Rebamipide concentration in ethanol was proportionally increased with the increment of TBPOH or NaOH added, indicating that the formation of complex with a counter ion should contribute to the solubilization of rebamipide in ethanol. Both Rebamipide-TBPOH complex (Reb-TBPOH) and Rebamipide-NaOH complex (Reb-NaOH) obtained by lyophilization showed no endothermic peak in DSC and no diffraction peak in XRPD, suggesting that the solid state of both complexes should be amorphous. Reb-TBPOH maintained the dissolution of rebamipide in SNEDDS vehicle (Capryol 90:Cremophor EL:Transcutol P = 4:3:3) at 20 mg/g at least for 28 days, while Reb-NaOH did it at 10 mg/g. In vitro dissolution study showed that Reb-TBPOH SNEDDS and Reb-NaOH SNEDDS containing rebamipide at 10 mg/g maintained the complete dissolution of rebamipide in FaSSIF (intestinal luminal condition). In the gastric luminal condition (pH3.9 acetate buffer), the high concentration, close to the complete dissolution, was transiently observed and quickly decreased to one-sixth of the maximum, but it was still around 70 times higher than that of the crystalline powder. The additional utilization of Eudragit EPO for SNEDDS preparations of both complexes successfully maintained the high concentrations of rebamipide in the gastric luminal condition. In vivo oral absorption studies clearly indicated that SNEDDS preparations utilizing Reb-counter ion complex successfully improved rebamipide absorption.

    DOI: 10.1016/j.ejps.2021.105721

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  • イオン輸送体を分子標的としたがん幹細胞の新規治療法の開発

    林 美樹夫, 海堀 昌樹, 松田 達志, 丸山 正人, 齊藤 朋人, 石田 光明, 岩田 亮一, 大江 総一, 中野 洋輔

    関西医科大学雑誌   71   32 - 33   2020.12

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    Language:Japanese   Publisher:関西医科大学医学会  

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  • BATTLE: Genetically Engineered Strategies for Split-Tunable Allocation of Multiple Transgenes in the Nervous System. Reviewed International journal

    Keigo Kohara, Akitoshi Inoue, Yousuke Nakano, Hirokazu Hirai, Takuya Kobayashi, Masato Maruyama, Ryosuke Baba, Chiho Kawashima

    iScience   23 ( 6 )   101248 - 101248   2020.6

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    Elucidating fine architectures and functions of cellular and synaptic connections requires development of new flexible methods. Here, we created a concept called the "battle of transgenes," based on which we generated strategies using genetically engineered battles of multiple recombinases. The strategies enabled split-tunable allocation of multiple transgenes. We demonstrated the versatility of these strategies and technologies in inducing strong and multi-sparse allocations of multiple transgenes. Furthermore, the combination of our transgenic strategy and expansion microscopy enabled three-dimensional high-resolution imaging of whole synaptic structures in the hippocampus with simultaneous visualizations of endogenous synaptic proteins. These strategies and technologies based on the battle of genes may accelerate the analysis of whole synaptic and cellular connections in diverse life science fields.

    DOI: 10.1016/j.isci.2020.101248

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  • ICOSLG-mediated regulatory T-cell expansion and IL-10 production promote progression of glioblastoma. Reviewed International journal

    Ryoichi Iwata, Joo Hyoung Lee, Mikio Hayashi, Umberto Dianzani, Kohei Ofune, Masato Maruyama, Souichi Oe, Tomoki Ito, Tetsuo Hashiba, Kunikazu Yoshimura, Masahiro Nonaka, Yosuke Nakano, Lyse Norian, Ichiro Nakano, Akio Asai

    Neuro-oncology   22 ( 3 )   333 - 344   2020.3

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    BACKGROUND: Targeting immune checkpoint proteins has recently gained substantial attention due to the dramatic success of this strategy in clinical trials for some cancers. Inducible T-cell co-stimulator ligand (ICOSLG) is a member of the B7 family of immune regulatory ligands, expression of which in cancer is implicated in disease progression due to regulation of antitumor adaptive immunity. Although aberrant ICOSLG expression has been reported in glioma cells, the underlying mechanisms that promote glioblastoma (GBM) progression remain elusive. METHODS: Here, we investigated a causal role for ICOSLG in GBM progression by analyzing ICOSLG expression in both human glioma tissues and patient-derived GBM sphere cells (GSCs). We further examined its immune modulatory effects and the underlying molecular mechanisms. RESULTS: Bioinformatics analysis and GBM tissue microarray showed that upregulation of ICOSLG expression was associated with poor prognosis in patients with GBM. ICOSLG expression was upregulated preferentially in mesenchymal GSCs but not in proneural GSCs in a tumor necrosis factor-α/nuclear factor-kappaB-dependent manner. Furthermore, ICOSLG expression by mesenchymal GSCs promoted expansion of T cells that produced interleukin-10. Knockdown of the gene encoding ICOSLG markedly reduced GBM tumor growth in immune competent mice, with a concomitant downregulation of interleukin-10 levels in the tumor microenvironment. CONCLUSIONS: Inhibition of the ICOSLG-inducible co-stimulator axis in GBM may provide a promising immunotherapeutic approach for suppressing a subset of GBM with an elevated mesenchymal signature.

    DOI: 10.1093/neuonc/noz204

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  • The involvement of cancer stem cells in brain metastatic process Invited Reviewed

    岩田亮一, 丸山正人, 大舟晃平, 中野洋輔, 大江総一, 林美樹夫, 吉村晋一, 埜中正博, 淺井昭雄

    Cytometry Research (Web)   28 ( 1 )   2018

  • Establishment of a tumor sphere cell line from a metastatic brain neuroendocrine tumor Reviewed

    Ryoichi Iwata, Masato Maruyama, Tomoki Ito, Yosuke Nakano, Yonehiro Kanemura, Taro Koike, Souichi Oe, Kunikazu Yoshimura, Masahiro Nonaka, Shosaku Nomura, Tetsuo Sugimoto, Hisao Yamada, Akio Asai

    MEDICAL MOLECULAR MORPHOLOGY   50 ( 4 )   211 - 219   2017.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s00795-017-0160-0

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  • Overview and assessment of the histochemical methods and reagents for the detection of beta-galactosidase activity in transgenic animals Reviewed

    Stefan Trifonov, Yuji Yamashita, Masahiko Kase, Masato Maruyama, Tetsuo Sugimoto

    ANATOMICAL SCIENCE INTERNATIONAL   91 ( 1 )   56 - 67   2016.1

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    DOI: 10.1007/s12565-015-0300-3

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  • Glutamic acid decarboxylase 1 alternative splicing isoforms: characterization, expression and quantification in the mouse brain Reviewed

    Stefan Trifonov, Yuji Yamashita, Masahiko Kase, Masato Maruyama, Tetsuo Sugimoto

    BMC NEUROSCIENCE   15 ( 1 )   Article No. 114   2014.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1186/1471-2202-15-114

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  • Lineage-specific purification of neural stem/progenitor cells from differentiated mouse induced pluripotent stem cells Reviewed

    Masato Maruyama, Yuji Yamashita, Masahiko Kase, Stefan Trifonov, Tetsuo Sugimoto

    Stem Cells Translational Medicine   2 ( 6 )   420 - 433   2013

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:John Wiley and Sons Ltd  

    DOI: 10.5966/sctm.2012-0139

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  • Aminopeptidase Q/Laeverin Reviewed

    Akira Hattori, Masato Maruyama, Hiroshi Fujiwara, Masafumi Tsujimoto

    Handbook of Proteolytic Enzymes   1   442 - 444   2013

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    Language:English   Publishing type:Part of collection (book)   Publisher:Elsevier Ltd  

    DOI: 10.1016/B978-0-12-382219-2.00089-2

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  • 中枢聴覚系のコリン線維とムスカリン性受容体(Cholinergic Fibers and Muscarinic Receptors in the Central Auditory System) Reviewed

    濱田 聡子, ステファン・トリフォノフ, 宝谷 剛志, 加瀬 政彦, 山下 雄司, 馬場 一泰, 清水 順一, 丸山 正人, 友田 幸一, 山下 敏夫, 杉本 哲夫

    関西医科大学雑誌   63   7-9 - 9   2012.11

  • Laeverin/aminopeptidase Q induces trophoblast invasion during human early placentation Reviewed

    Akihito Horie, Hiroshi Fujiwara, Yukiyasu Sato, Koh Suginami, Hisanori Matsumoto, Masato Maruyama, Ikuo Konishi, Akira Hattori

    HUMAN REPRODUCTION   27 ( 5 )   1267 - 1276   2012.5

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    DOI: 10.1093/humrep/des068

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  • Lateral regions of the rodent striatum reveal elevated glutamate decarboxylase 1 mRNA expression in medium-sized projection neurons Reviewed

    Stefan Trifonov, Takeshi Houtani, Masahiko Kase, Kazunori Toida, Masato Maruyama, Yuji Yamashita, Jun-Ichi Shimizu, Tetsuo Sugimoto

    EUROPEAN JOURNAL OF NEUROSCIENCE   35 ( 5 )   711 - 722   2012.3

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    DOI: 10.1111/j.1460-9568.2012.08001.x

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  • Histological determination of the areas enriched in cholinergic terminals and m2 and m3 muscarinic receptors in the mouse central auditory system Reviewed

    Hamada S, Houtani T, Trifonov S, Kase M, Maruyama M, Shimizu J, Yamashita T, Tomoda K, Sugimoto T

    The Anatomical Record   293 ( 8 )   1393-1399   2010.8

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    DOI: 10.1002/ar.21186

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  • Histological Determination of the Areas Enriched in Cholinergic Terminals and m2 and m3 Muscarinic Receptors in the Mouse Central Auditory System Reviewed

    Satoko Hamada, Takeshi Houtani, Stefan Trifonov, Masahiko Kase, Masato Maruyama, Jun-Ichi Shimizu, Toshio Yamashita, Koichi Tomoda, Tetsuo Sugimoto

    ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY   293 ( 8 )   1393 - 1399   2010.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/ar.21186

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  • GPR155: Gene organization, multiple mRNA splice variants and expression in mouse central nervous system Reviewed

    Stefan Trifonov, Takeshi Houtani, Jun-ichi Shimizu, Satoko Hamada, Masahiko Kase, Masato Maruyama, Tetsuo Sugimoto

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   398 ( 1 )   19 - 25   2010.7

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    DOI: 10.1016/j.bbrc.2010.05.162

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  • Histidine 379 of Human Laeverin/Aminopeptidase Q, a Nonconserved Residue within the Exopeptidase Motif, Defines Its Distinctive Enzymatic Properties Reviewed

    Masato Maruyama, Naomi Arisaka, Yoshikuni Goto, Yosuke Ohsawa, Hideshi Inoue, Hiroshi Fujiwara, Akira Hattori, Masafumi Tsujimoto

    JOURNAL OF BIOLOGICAL CHEMISTRY   284 ( 50 )   34692 - 34702   2009.12

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    DOI: 10.1074/jbc.M109.066712

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  • 中枢聴覚路におけるコリン線維とムスカリン性受容体サブタイプ発現の解析 Reviewed

    濱田聡子, 宝谷剛志, Trifonov Stefan, 丸山正人, 加瀬政彦, 堤 俊之, 杉本哲夫, 友田幸一, 山下敏夫

    頭頸部自律神経   23   72-73 - 73   2009.5

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  • Effects of Methyl-beta-cyclodextrin Treatment on Secretion Profile of Interferon-beta and Zonula Occuludin-1 Architecture in Madin-Darby Canine Kidney Cell Monolayers Reviewed

    Masato Maruyama, Kayo Ishida, Yoshihiko Watanabe, Makiya Nishikawa, Yoshinobu Takakura

    BIOLOGICAL & PHARMACEUTICAL BULLETIN   32 ( 5 )   910 - 915   2009.5

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  • IN SITU HYBRIDIZATION STUDY OF THE DISTRIBUTION OF CHOLINE ACETYLTRANSFERASE mRNA AND ITS SPLICE VARIANTS IN THE MOUSE BRAIN AND SPINAL CORD Reviewed

    S. Trifonov, T. Houtani, S. Hamada, M. Kase, M. Maruyama, T. Sugimoto

    NEUROSCIENCE   159 ( 1 )   344 - 357   2009.3

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    DOI: 10.1016/j.neuroscience.2008.12.054

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  • Biochemical and enzymatic properties of the M1 family of aminopeptidases involved in the regulation of blood pressure Reviewed

    Masafumi Tsujimoto, Yoshikuni Goto, Masato Maruyama, Akira Hattori

    HEART FAILURE REVIEWS   13 ( 3 )   285 - 291   2008.9

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    DOI: 10.1007/s10741-007-9064-8

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  • Laeverin/aminopeptidase Q, a novel bestatin-sensitive leucine aminopeptidase belonging to the M1 family of aminopeptidases Reviewed

    Masato Maruyama, Akira Hattori, Yoshikuni Goto, Masamichi Ueda, Michiyuki Maeda, Hiroshi Fujiwara, Masafumi Tsujimoto

    JOURNAL OF BIOLOGICAL CHEMISTRY   282 ( 28 )   20088 - 20096   2007.7

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    DOI: 10.1074/jbc.M702650200

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  • Cholesterol is required for the polarized secretion of erythropoietin in Madin-Darby canine kidney cells Reviewed

    M Maruyama, M Kishimoto, K Ishida, Y Watanabe, M Nishikawa, S Masuda, R Sasaki, Y Takakura

    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS   438 ( 2 )   174 - 181   2005.6

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    DOI: 10.1016/j.abb.2005.04.005

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  • Secretion mode and subcellular localization of human interferon-beta exogenously expressed in porcine renal epithelial LLC-PK1 cells Reviewed

    T Nishio, M Maruyama, T Yoshida, Y Watanabe, M Nishikawa, Y Takakura

    BIOLOGICAL & PHARMACEUTICAL BULLETIN   27 ( 10 )   1653 - 1655   2004.10

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  • Simultaneous detection of DsRed2-tagged and EGFP-tagged human beta-interferons in the same single cells Reviewed

    M Maruyama, T Nishio, T Yoshida, C Ishida, K Ishida, Y Watanabe, M Nishikawa, Y Takakura

    JOURNAL OF CELLULAR BIOCHEMISTRY   93 ( 3 )   497 - 502   2004.10

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    DOI: 10.1002/jcb.20203

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  • Subcellular trafficking of exogenously expressed interferon-beta in Madin-Darby canine kidney cells Reviewed

    M Maruyama, T Nishio, T Kato, T Yoshida, C Ishida, Y Watanabe, M Nishikawa, Y Kaneda, Y Takakura

    JOURNAL OF CELLULAR PHYSIOLOGY   201 ( 1 )   117 - 125   2004.10

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    DOI: 10.1002/jcp.20038

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  • Expression and visualization of a human interferon-beta-enhanced green fluorescent protein chimeric molecule in cultured cells Reviewed

    M Nakamura, M Maruyama, F Yamashita, Y Takakura, M Hashida, Y Watanabe

    BIOLOGICAL & PHARMACEUTICAL BULLETIN   27 ( 3 )   411 - 414   2004.3

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  • Therapeutic effect of intravenous delivery of lipoplexes containing the interferon-beta gene and poly I : poly C in a murine lung metastasis model Reviewed

    F Sakurai, T Terada, M Maruyama, Y Watanabe, F Yamashita, Y Takakura, M Hashida

    CANCER GENE THERAPY   10 ( 9 )   661 - 668   2003.9

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    DOI: 10.1038/sj.cgt.7700617

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  • Secretion polarity of interferon-beta in epithelial cell lines Reviewed

    K Nakanishi, Y Watanabe, M Maruyama, F Yamashita, Y Takakura, M Hashida

    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS   402 ( 2 )   201 - 207   2002.6

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  • Modulation of intestinal permeability by nitric oxide donors: Implications in intestinal delivery of poorly absorbable drugs Reviewed

    A Yamamoto, H Tatsumi, M Maruyama, T Uchiyama, N Okada, T Fujita

    JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS   296 ( 1 )   84 - 90   2001.1

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  • Nitric oxide donors enhance the absorption of water soluble drugs across the rat colonic membrane

    A. Yamamoto, H. Tatsumi, M. Maruyama, N. Okada, T. Fujita

    Proceedings of the Controlled Release Society   ( 26 )   357 - 358   1999

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Books

  • Aminopeptidase Q/Laveverin「Handbook of Proteolytic Enzymes」

    Hattori A, Maruyama M, Fujiwara H, Tsujimoto M( Role: Joint author)

    Academic Press  2013  ( ISBN:9780123822192

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    Responsible for pages:442-444   Book type:Scholarly book

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MISC

  • イオン輸送体を分子標的としたがん幹細胞の新規治療法の開発

    林 美樹夫, 海堀 昌樹, 松田 達志, 丸山 正人, 岩田 亮一, 齊藤 朋人, 中野 洋輔

    関西医科大学雑誌   69   46 - 46   2018.12

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  • ヒト神経膠芽腫由来U87MG細胞から樹立した高い腫瘍形成能を持つスフェロイドはPC3-Secreted Microproteinを高発現する

    丸山 正人, 中野 洋輔, 岩田 亮一, 西村 拓也, 加瀬 政彦, 杉本 哲夫

    生命科学系学会合同年次大会   2017年度   [3P - 0964]   2017.12

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  • DiI注入法によるBcas1 in situ hybridization陽性細胞の形態描出

    加瀬 政彦, 山下 雄司, Trifonov Stefan, 丸山 正人, 杉本 哲夫

    日本組織細胞化学会総会・学術集会講演プログラム・予稿集   56回   82 - 82   2015.10

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  • 神経幹細胞特異的に発現させた薬剤耐性遺伝子によるマウスiPS細胞由来神経幹細胞の純化

    丸山 正人, 山下 雄司, Trifonov Stefan, 加瀬 政彦, 杉本 哲夫

    日本薬学会年会要旨集   133年会 ( 3 )   206 - 206   2013.3

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  • マウス線条体のV字型縞構造

    山下 雄司, ステファン・トリフォノフ, 宝谷 剛志, 丸山 正人, 加瀬 政彦, 杉本 哲夫

    解剖学雑誌   87 ( 2 )   38 - 38   2012.6

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  • 薬剤選択によるマウスiPS細胞由来神経幹細胞の純化

    丸山 正人, 山下 雄司, トリフォノフ・ステファン, 加瀬 政彦, 杉本 哲夫

    日本生化学会大会プログラム・講演要旨集   84回   3P - 0400   2011.9

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  • Reconstruction of calbindin-poor lateral striatum in the mouse

    Yuji Yamashita, Stefan Trifonov, Takeshi Houtani, Masato Maruyama, Masahiko Kase, Jun-ichi Shimizu, Tetsuo Sugimoto

    NEUROSCIENCE RESEARCH   71   E140 - E140   2011

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    DOI: 10.1016/j.neures.2011.07.605

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  • Purification of neural stem cells derived from mouse induced pluripotent stem cells by drug selection

    Masato Maruyama, Yuji Yamashita, Stefan Trifonov, Masahiko Kase, Jun-ichi Shimizu, Tetsuo Sugimoto

    NEUROSCIENCE RESEARCH   71   E330 - E330   2011

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    DOI: 10.1016/j.neures.2011.07.1443

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  • GPR155-遺伝子の構成とマウス脳内mRNA発現の解析(GPR155: gene organization and expression in mouse brain)

    Trifonov Stefan, 宝谷 剛志, 清水 順一, 山下 雄司, 濱田 聡子, 加瀬 政彦, 丸山 正人, 杉本 哲夫

    神経化学   49 ( 2-3 )   548 - 548   2010.8

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  • ラット中枢神経系におけるPMES-2遺伝子発現細胞の特徴的な分布

    加瀬 政彦, 宝谷 剛志, 丸山 正人, トリフォノフ・ステファン, 清水 順一, 杉本 哲夫

    解剖学雑誌   85 ( Suppl. )   189 - 189   2010.3

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  • GPR155-gene organization and expression in mouse brain

    Stefan Trifonov, Takeshi Houtani, Jun-ichi Shimizu, Yuji Yamashita, Satoko Hamada, Masahiko Kase, Masato Maruyama, Tetsuo Sugimoto

    NEUROSCIENCE RESEARCH   68   E147 - E147   2010

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    DOI: 10.1016/j.neures.2010.07.2224

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  • 薬剤選択を利用したマウス人工多能性幹細胞由来神経幹細胞の効率的純化

    丸山 正人, 室谷 剛志, Trifonov Stefan, 加瀬 政彦, 杉本 哲夫

    日本生化学会大会プログラム・講演要旨集   82回   2P - 760   2009.9

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  • Choline acetyltransferase mRNA splice variants マウス脳・脊髄での発現

    トリフォノフ・ステファン, 宝谷 剛志, 濱田 聡子, 加瀬 政彦, 丸山 正人, 杉本 哲夫

    解剖学雑誌   84 ( Suppl. )   249 - 249   2009.3

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  • Effective purification of neural stem cells derived from mouse induced pluripotent stem cells

    Masato Maruyama, Takeshi Houtani, Stefan Trifonov, Masahiko Kase, Tetsuo Sugimoto

    NEUROSCIENCE RESEARCH   65   S93 - S93   2009

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    DOI: 10.1016/j.neures.2009.09.389

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  • Distinct regional differences in the expression pattern of the transcripts of the cholinergic gene locus

    Stefan Trifonov, Takeshi Houtani, Satoko Hamada, Masahiko Kase, Masato Maruyama, Jun-ichi Shimizu, Tetsuo Sugimoto

    NEUROSCIENCE RESEARCH   65   S75 - S75   2009

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    DOI: 10.1016/j.neures.2009.09.267

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  • M1アミノペプチダーゼファミリーの生理的/病理学的機能

    服部明, 服部明, 丸山正人, 後藤芳邦, 辻本雅文

    ケミカルバイオロジーシンポジウム発表要旨集 第4回 化学-生物融合領域創成の軌跡 理研シンポジウム 平成20年   2008

  • インターフェロン・GFP融合タンパクの極性上皮細胞内輸送過程の解析

    丸山 正人, 石田 智早希, 渡部 好彦, 高倉 喜信

    薬剤学 = Journal of Pharmaceutical Science and Technology, Japan   63   125 - 125   2003.3

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  • 腎上皮細胞LLC-PK_1におけるインターフェロンの細胞内動態と分泌方向性の解析

    吉田 豊一, 西尾 照子, 丸山 正人, 渡部 好彦, 高倉 喜信

    薬剤学 = Journal of Pharmaceutical Science and Technology, Japan   63   126 - 126   2003.3

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Presentations

  • Paracellular routeを介した薬物透過性に及ぼす腸神経系の影響に関する基礎的研究

    吉形 南美, 坂口 真菜, 大河原 賢一, 丸山 正人, 檜垣 和孝

    日本薬剤学会年会講演要旨集  2020.5  (公社)日本薬剤学会

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    Event date: 2020.5

    Language:Japanese  

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  • ポリアミン誘導体を用いた薬物吸収改善の機構に関する基礎的研究

    渡邊 菜摘, 小林 紘子, 三宅 正晃, 大河原 賢一, 丸山 正人, 檜垣 和孝

    日本薬剤学会年会講演要旨集  2020.5  (公社)日本薬剤学会

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    Event date: 2020.5

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  • Self-nanoemulsifying Drug Delivery Systemによる難水溶性薬物Clofazimineの経口吸収挙動改善とその機構解析に関する研究

    山之内 慶太, 石丸 智基, 大河原 賢一, 丸山 正人, 檜垣 和孝

    日本薬剤学会年会講演要旨集  2020.5  (公社)日本薬剤学会

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  • パクリタキセル内封リポソーム製剤の転移性乳がんモデルマウスにおける抗腫瘍効果に及ぼす血管正常化の影響

    松井 はづき, 高杉 裕太, 大河原 賢一, 丸山 正人, 檜垣 和孝

    日本薬剤学会年会講演要旨集  2020.5  (公社)日本薬剤学会

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  • ドキソルビシン内封リポソーム製剤の悪性黒色腫固形がん治療に及ぼす薬物放出特性の影響

    家中 悠輔, 東條 遥佳, 兵頭 健治, 石原 比呂之, 菊池 寛, 大河原 賢一, 丸山 正人, 檜垣 和孝

    日本薬剤学会年会講演要旨集  2020.5  (公社)日本薬剤学会

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  • 難溶解性薬物イブプロフェン及びラロキシフェンの経口吸収挙動の解析と予測

    近藤敬二, 河津翔, 妹尾遼太郎, 大河原賢一, 丸山正人, 檜垣和孝

    日本薬物動態学会第34回年会 

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    Event date: 2019.12.9 - 2019.12.12

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  • 血管新生阻害剤SU-5416による血管正常化療法ががん関連繊維芽細胞に及ぼす影響

    中村 遥, 松井 はづき, 高杉 裕太, 大河原 賢一, 丸山 正人, 檜垣 和孝

    第38回日本DDS学会学術集会  2022.6.29 

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  • マウス乳がん由来4T1細胞におけるがん幹細胞様のモデル細胞株樹立に関する研究

    瀬口 実穂, 丸山 正人, 檜垣 和孝

    第38回日本DDS学会学術集会  2022.6.29 

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  • ドキソルビシン(DOX)耐性腫瘍に対するDOX-PEGリポソームの抗腫瘍効果に及ぼす腫瘍組織内血管内皮細胞特性の影響

    上田 智樹, 家中 悠輔, 大河原 賢一, 丸山 正人, 檜垣 和孝

    日本薬剤学会第37年会  2022.5.27 

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  • 血管新生阻害剤 SU5416 - パクリタキセル (PTX) 内封 PEG リポソームの 逐次的頻回併用療法の転移性乳がん4T1担がんマウスにおける抗腫瘍効果

    鳥井 怜冶, 松井 はづき, 高杉 裕太, 大河原 賢一, 丸山 正人, 檜垣 和孝

    日本薬剤学会第37年会  2022.5.27 

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  • 難水溶性薬物ClofazimineのSelf-nanoemulsifying Drug Delivery System(SNEDDS)による経口吸収挙動改善機構の解析

    林 佳佑, 山之内 慶太, 石丸 智基, 丸山 正人, 檜垣 和孝

    日本薬剤学会第37年会  2022.5.26 

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  • 初代培養腸神経系細胞―Caco-2 細胞共培養系を用いた腸神経系による腸上皮細胞透過性制御に関する基礎的研究: 受動拡散による薬物透過に関する検討

    枠島 静, 吉形 南美, 丸山 正人, 檜垣 和孝

    日本薬剤学会第37年会  2022.5.26 

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  • 難水溶性-難脂溶性薬物メベンダゾールのカウンターイオンを用いたSNEDDS (Self-nanoemulsifying Drug Delivery System)製剤化と吸収挙動改善に関する研究

    井上 知也, 住元 祐介, 大川 慎也, 丸山 正人, 檜垣 和孝

    日本薬剤学会第36年会  2021.5.14 

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  • ポリアミン誘導体OPI-331を用いた新規吸収改善製剤開発のための基礎的研究

    武林 翔, 宮里 萌花, 渡邊 菜摘, 三宅 正晃, 大河原賢一, 丸山正人, 檜垣 和孝

    日本薬剤学会第36年会  2021.5.13 

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  • Self-nanoemulsifying Drug Delivery System による難水溶性薬物 Resveratrol の経口吸収改善とその機構解析に関する研究

    竹本 雄貴, 近藤 敬二, 丸山 正人, 檜垣 和孝

    日本薬剤学会第36年会  2021.5.13 

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  • ヒト神経膠芽腫由来U87MG細胞から樹立した高い腫瘍形成能を持つスフェロイドはPC3-Secreted Microproteinを高発現する

    丸山正人, 中野洋輔, 岩田亮一, 西村拓也, 加瀬政彦, 杉本哲夫

    2017年度生命科学系学会合同年次大会(ConBio2017)第40回日本分子生物学会年会 第90回日本生化学会大会  2017.12.8 

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  • 神経内分泌腫瘍の転移脳組織からの癌幹細胞

    岩田亮一, 丸山正人, 伊藤量基, 中野洋輔, 小池太郎, 大江聡一, 吉村晋一, 埜中正博, 野村昌作, 杉本哲夫, 山田久夫, 淺井昭雄

    第49回日本臨床分子形態学会総会・学術集会  2017.9 

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    Venue:岐阜  

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  • 転移性脳腫瘍からのがん幹細胞株樹立

    岩田亮一, 丸山正人, 中野洋輔, 小池太郎, 大江聡一, 吉村晋一, 埜中正博, 杉本哲夫, 山田久夫, 淺井昭雄

    第27回日本サイトメトリー学会学術集会  2017.6 

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    Venue:神戸  

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  • 成熟ラット脳におけるBcas1 mRNA発現細胞の免疫組織化学的および形態学的解析

    加瀬政彦, 山下雄司, 中野洋輔, 丸山正人, 杉本哲夫

    第122回日本解剖学会総会・全国学術集会  2017.3 

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    Venue:長崎  

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  • マウス線条体のGPR155陽性interneuron

    山下雄司, Trifonov Stefan, 丸山正人, 加瀬政彦, 中野洋輔, 杉本哲夫

    第122回日本解剖学会総会・全国学術集会  2017.3 

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    Venue:長崎  

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  • マウス脳GPR155の機能解明へのアプローチ

    山下雄司, Trifonov Stefan, 加瀬政彦, 丸山正人, 中野洋輔, 西村拓也, 杉本哲夫

    第39回日本神経科学大会  2016.7 

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    Venue:横浜  

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  • マウス線条体外側部のD1ニューロンはGPR155を発現している

    山下雄司, Trifonov Stefan, 丸山正人, 加瀬政彦, 杉本哲夫

    第121回日本解剖学会総会・全国学術集会  2016.3 

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    Venue:郡山  

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  • DiI注入法によるBcas1 in situ hybridization陽性細胞の形態抽出

    加瀬政彦, 山下雄司, Trifonov Stefan, 丸山正人, 杉本哲夫

    第56回日本組織細胞化学会総会・学術集会  2015.10 

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    Venue:枚方  

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  • In situ hybridization標識細胞の総合的解析法

    加瀬政彦, 山下雄司, Trifonov Stefan, 丸山正人, 杉本哲夫

    第120回日本解剖学会総会・全国学術集会 第92回日本生理学会大会 合同大会  2015.3 

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    Venue:神戸  

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  • 線条体外側部の機能構築

    山下雄司, Trifonov Stefan, 丸山正人, 加瀬政彦, 杉本哲夫

    高次機能代謝系・ブレインメディカルサイエンス系大学院生研究中間発表会  2014.7 

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    Venue:枚方  

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  • 機械的障害後の成熟ラット脳におけるBcas1遺伝子発現細胞の分布変化

    加瀬政彦, 丸山正人, トリフォノフ ステファン, 山下雄司, 杉本哲夫

    第119回日本解剖学会総会・全国学術集会  2014.3 

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    Venue:下野  

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  • マウスGPR155陽性線条体ニューロンの共存物質

    山下雄司, 丸山正人, トリフォノフ ステファン, 加瀬政彦, 杉本哲夫

    日本解剖学会第89回近畿支部学術集会  2013.11 

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    Venue:生駒  

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  • 神経幹細胞特異的に発現させた薬剤耐性遺伝子によるマウスiPS細胞由来神経幹細胞の純化

    丸山正人, 山下雄司, Trifonov Stefan, 加瀬政彦, 杉本哲夫

    日本薬学会第133年会  2013.3 

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    Venue:横浜  

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  • GPR抗体を用いたマウス線条体外側部V字型縞構造の解析

    山下雄司, Trifonov Stefan, 丸山正人, 加瀬政彦, 杉本哲夫

    第118回日本解剖学会総会・全国学術集会  2013.3 

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    Venue:高松  

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  • マウス線条体外側部マーカー物質のV字型縞構造への局在

    山下雄司, Trifonov Stefan, 宝谷剛志, 丸山正人, 加瀬政彦, 杉本哲夫

    第117回日本解剖学会総会・全国学術集会  2012.3 

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    Venue:甲府  

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  • 成獣ラット脳の機械的外傷後にみられる傷周辺組織でのPMES-2遺伝子発現細胞の増加

    加瀬政彦, 丸山正人, Trifonov Stefan, 山下雄司, 杉本哲夫

    第117回日本解剖学会総会・全国学術集会  2012.3 

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    Venue:甲府  

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  • マウス線条体のV字型縞構造

    山下雄司, ステファン トリフォノフ, 宝谷剛志, 丸山正人, 加瀬政彦, 杉本哲夫

    第87回日本解剖学会近畿支部学術集会  2011.12 

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    Venue:西宮  

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  • マウス線条体の区分

    山下雄司, 丸山正人, 加瀬政彦, Trifonov Stefan, 清水順一, 杉本哲夫

    第9回近畿大学環境科学研究会  2010.8 

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    Venue:東大阪  

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  • ラット中枢神経系におけるPMES-2遺伝子発現細胞の特徴的な分布

    Kase M, Houtani T, Maruyama M, Trifonov S, Shimizu J, Sugimoto T

    第115回日本解剖学会総会・全国学術集会  2010.3 

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    Venue:盛岡  

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  • 薬剤選択を利用したマウス人工多能性幹細胞由来神経幹細胞の効率的純化

    Maruyama M, Houtani T, Trifonov S, Kase M, Sugimoto T

    第82回日本生化学大会  2009.10 

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    Venue:神戸  

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  • マウスiPS細胞から分化させた神経幹細胞の効率的純化

    Maruyama M, Houtani T, Trifonov S, Kase M, SugimotoT

    第32回日本神経科学大会  2009.9 

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    Venue:名古屋  

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  • Choline acetyltransferase mRNA splice variants -マウス脳・脊髄での発現

    Trifonov S, Houtani T, Hamada S, Kase M, Maruyama M, Sugimoto T

    第114回日本解剖学会総会・全国学術集会  2009.3 

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    Venue:岡山  

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  • 点変異導入によるヒトLaeverinのHis379残基の機能解析

    有坂尚美, 丸山正人, 服部明, 辻本雅文

    第31回日本分子生物学会年会・第81回日本生化学会大会合同大会  2008.12 

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    Venue:神戸  

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  • Expression of ChAT mRNA splice variants in mouse CNS

    Stefan Trifonov, 宝谷剛志, 濱田聡子, 加瀬政彦, 丸山正人, 杉本哲夫

    第84回日本解剖学学会近畿支部学術集会  2008.11 

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    Venue:大阪  

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  • ヒトLaeverinの酵素学的性状の発現におけるHis379残基の役割

    有坂尚美, 丸山正人, 服部明, 辻本雅文

    病態と治療におけるプロテアーゼとインヒビター学会  2008.8 

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    Venue:大阪  

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  • 中枢聴覚路におけるコリン線維とムスカリン性受容体サブタイプ発現の解析

    濱田聡子, 宝谷剛志, Stefan Trifonov, 丸山正人, 加瀬政彦, 堤俊之, 杉本哲夫, 友田幸一, 山下敏夫

    第26回頭頚部自律神経研究会  2008.8 

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    Venue:大阪  

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  • ヒトLaeverin/Aminopeptidase QのHis379残基の機能解析

    丸山正人, 有坂尚美, 服部 明, 辻本雅文

    日本薬学会第128年会  2008.3 

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    Venue:横浜、日本  

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  • 新規M1アミノペプチダーゼLaeverinの酵素学的性状の解析

    丸山正人, 服部明, 辻本雅文

    第4回ケミカルバイオロジーシンポジウム  2008.2 

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    Presentation type:Symposium, workshop panel (nominated)  

    Venue:静岡、日本  

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  • 新規ロイシンアミノペプチダーゼLaeverinの機能解析

    服部 明, 丸山 正人, 辻本雅文

    第30回日本分子生物学会年会第80回日本生化学会大会合同大会  2007.12 

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    Presentation type:Symposium, workshop panel (nominated)  

    Venue:横浜、日本  

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  • 新規アミノペプチダーゼLaeverinの酵素学的性状の解析

    丸山 正人, 服部 明, 辻本 雅文

    第30回日本分子生物学会年会第80回日本生化学会大会合同大会  2007.12 

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    Venue:横浜、日本  

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  • Laeverin: a novel bestatin sensitive leucine aminopeptidase belonging to the M1 family of aminopeptidases International conference

    Masato Maruyama, Akira Hattori, Masafumi Tsujimoto

    5th General Meeting of the International Proteolysis Society  2007.10 

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    Venue:Patras, Greece  

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  • 新規酵素Laeverinの酵素学的性状解析

    丸山正人, 服部 明, 辻本雅文

    第12回病態と治療におけるプロテアーゼとインヒビター学会  2007.8 

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    Venue:大阪、日本  

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  • Substance P stimulates the cell surface translocation of angiotensin IV receptor/placental leucine aminopeptidase in PC12 cells

    Masato Maruyama, Akira Hattori, Masafumi Tsujimoto

    RIKEN International Symposium on Chemical Biology  2007.1 

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    Venue:Kanagawa, Japan  

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  • サブスタンスPは神経細胞においてアンギオテンシンIV受容体/胎盤性ロイシンアミノペプチダーゼの細胞膜移行を誘導する

    丸山正人, 服部 明, 辻本雅文

    第28回生体膜と薬物の相互作用シンポジウム  2006.11 

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    Presentation type:Symposium, workshop panel (nominated)  

    Venue:静岡、日本  

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  • サブスタンスPによる胎盤性ロイシンアミノペプチダーゼの細胞膜移行誘導

    丸山正人, 服部 明, 辻本雅文

    第11回病態と治療におけるプロテアーゼとインヒビター研究会  2006.8 

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    Venue:仙台、日本  

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  • 上皮細胞に発現させたインターフェロンの分泌方向性制御の試み

    石田香代, 吉田豊一, 丸山正人, 渡部好彦, 西川元也, 高倉喜信

    日本薬学会第125年会  2005.3 

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    Venue:東京、日本  

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  • 極性上皮細胞へのインターフェロン遺伝子導入後の分泌方向性に関する研究

    丸山正人

    創剤フォーラム第10回若手研究会  2004.11 

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    Venue:京都、日本  

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  • Mechanism of Polarized Secretion Erythropoietin in Mardin-Darby Canine Kidney Cells International conference

    Kayo Ishida, Masato Maruyama, Yoshihiko Watanabe, Yoshinobu Takakura

    5th Globalization of Pharmaceutics Education Network  2004.5 

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    Venue:Kyoto, Japan  

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  • Effects of cholesterol depletion on secretion polarity of human interferon-  in Mardin-Darby canine kidney cells International conference

    Kayo Ishida, Masato Maruyama, Yoshihiko Watanabe, Yoshinobu Takakura

    2nd World Congress of the Board of Pharaceutical Sciences of FIP  2004.5 

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    Venue:Kyoto, Japan  

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  • 上皮細胞におけるインターフェロンの細胞内輸送動態の解析:分泌方向性制御を目指して

    丸山正人, 石田智早希, 渡部好彦, 高倉喜信

    日本DDS学会第19年会  2003.6 

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    Venue:京都、日本  

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  • インターフェロン・GFP融合タンパクの極性上皮細胞内輸送過程の解析

    丸山正人, 石田智早希, 渡部好彦, 高倉喜信

    日本薬剤学会第18年会  2003.4 

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    Venue:京都、日本  

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  • 腎上皮細胞 LLC-PK1におけるインターフェロンの細胞内動態と分泌方向性の解析

    吉田豊一, 西尾照子, 丸山正人, 渡部好彦, 高倉喜信

    日本薬剤学会第18年会  2003.4 

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    Venue:京都、日本  

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  • 細胞内動態解析ツールとしてのインターフェロン・DsRed 融合タンパク発現ベクターの構築

    丸山正人, 西尾照子, 石田智早希, 渡部好彦, 高倉喜信

    日本薬学会第123年会  2003.3 

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    Venue:長崎、日本  

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  • Analysis of secretion polarity after gene delivery to epithelial cells International conference

    Masato Maruyama, Yoshihiko Watanabe, Yoshinobu Takakura

    4th Globalization of Pharmaceutics Education Network  2002.11 

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    Venue:Michigan, USA  

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  • 腎上皮細胞LLC-PK 1におけるインターフェロン・GFP融合タンパクの細胞内動態と分泌方向性の解析

    西尾照子, 丸山正人, 渡部好彦, 高倉喜信

    日本薬物動態学会第17年会  2002.11 

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    Venue:千葉、日本  

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  • Secretion polarity and intracellular transport of interferon-GFP fusion protein after gene delivery to epithelial cells International conference

    Masato Maruyama, Toyokazu Yoshida, Yoshihiko Watanabe, Yoshinobu Takakura

    29th Annual Meeting and Exposition of the Controlled Release Society  2002.7 

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    Venue:Seoul, Korea  

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  • 上皮細胞におけるインターフェロン・GFP 融合タンパクの細胞内輸送過程の解析

    丸山正人, 吉田豊一, 渡部好彦, 高倉喜信

    日本薬学会第122年会  2002.3 

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    Venue:千葉、日本  

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  • 上皮細胞をターゲットとしたインターフェロン遺伝子デリバリーとその分泌方向性: GFP 融合タンパクを用いた解析

    丸山正人, 加藤貴子, 渡部好彦, 高倉喜信

    日本薬学会第121年会  2001.3 

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    Venue:札幌、日本  

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  • 極性を有する上皮細胞へのインターフェロン遺伝子のデリバリーとそのエキソサイトーシス方向性

    丸山正人, 中西貴代, 渡部好彦, 橋田 充, 高倉喜信

    第22回生体膜と薬物の相互作用シンポジウム  2000.11 

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    Presentation type:Symposium, workshop panel (nominated)  

    Venue:京都、日本  

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  • Polarized secretion of IFN-beta in epithelial cells following gene transfer with IFN-beta encoding plasmid International conference

    Masato Maruyama, Kiyo Nakanishi, Yoshihiko Watanabe, Mitsuru Hashida, Yoshinobu Takakura

    Millennial World Congress of Pharmaceutical Sciences  2000.4 

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    Venue:San Francisco, USA  

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Awards

  • ポスター奨励賞

    2006.8   病態と治療におけるプロテアーゼとインヒビター研究会  

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Research Projects

  • Effect of ENS dysfunction on drug absorption from small intestine

    Grant number:20K07176  2020.04 - 2025.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    檜垣 和孝, 丸山 正人, 大河原 賢一

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    Grant amount:\4160000 ( Direct expense: \3200000 、 Indirect expense:\960000 )

    申請者らは、これまでに5-HT代謝異常ラットにおいて、i) Cephalexin (CEX) の経口投与後の吸収が増大すること, ii)CEXの吸収の一翼を担うPEPT1の小腸における発現は、粘膜ホモジネート中では変化が認められなかったが、小腸上皮細胞刷子縁膜上のPEPT1は有意に減少しており、PEPT1を介した膜透過はむしろ低下していること、iii)細胞間隙経路を介した受動拡散による膜透過が増大し、特に回腸において有意な増大となっていること、等を明らかにしてきた。本年度は、5-HT代謝異常ラットにおけるgastrointestinal transitの変動の可能性を検討し、前年までに明らかにした膜透過性変動との関係から、経口投与後の吸収性変動について考察を試みた。難水溶性色素phenol redをマーカーとして胃排出挙動を、また微小なガラスビーズをマーカーとして小腸内移行性を評価したところ、胃、十二指腸、空腸上部では、それぞれの消化管部位における移行性が亢進傾向にあること、一方で、空腸下部、回腸上部、回腸下部では、逆に移行性の低下、即ち滞留性が増大傾向にあることが明らかとなった。これは、CEXの透過亢進が起こっている回腸において滞留性が増大していることを意味しており、このことが経口投与後のCEX吸収増大を促したものと考えられた。また、CEXを5-HT代謝異常ラットに静脈内投与し、吸収過程以外の過程におけるCEXの動態変動の可能性を検討した。その結果、分布や血漿中からの初期の消失には、変動は認められなかったが、血漿中からの消失相における消失が、有意に遅延していることが明らかとなった。CEXは、腎臓の近位尿細管に存在しているPEPT2により再吸収されていることが知られていることから、腎の刷子縁膜上に発現しているPEPT2をWestern blot法により定量的に評価した。その結果、有意な減少が認められたことから、PEPT2を介した再吸収の低下が、血漿中からの消失の遅延の要因の一つと考えられた。

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  • Establishment of a novel drug delivery system for targeting cancer stem cells based on the structual analysis of tumor blood vesseles.

    Grant number:20K07155  2020.04 - 2023.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    丸山 正人, 檜垣 和孝

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    Grant amount:\4290000 ( Direct expense: \3300000 、 Indirect expense:\990000 )

    本研究では、腫瘍血管の構造解析に基づいたがん幹細胞への新規薬物送達法を確立し、がん幹細胞を標的とした新規治療法の開発を目指している。昨年度は、マウス4T1乳がん細胞を用いて、乳がん幹細胞の候補となり得るクローン株を複数(4種類)樹立した。そこで、本年度は、樹立したこれらのクローン株が、がん幹細胞の特性を有することを明らかにすることを目的に、腫瘍形成能とがん幹細胞マーカーの発現について、検討を行った。
    樹立した細胞を、5週齢のBalb/cマウスの皮下に1x106個の細胞を移植し、腫瘍形成能を評価したところ、移植した4種類のクローン株のうち、2種類のクローン株において、親株に比べて有意に高い腫瘍形成能を示すことが確認された。さらに、細胞数を5x105個に少なくして投与した場合についても検討を行った結果、1x106個の細胞を移植したときに高い腫瘍形成能を示した2種類のクローン株が、5x105個の細胞を投与した時にも、親株に比べて有意に高い腫瘍形成能を示すことが確認されたため、これらのクローン株が、がん幹細胞の有用な候補と考えられた。
    次に、がん幹細胞マーカーの発現について検討を行った。樹立した細胞は、ALDH1A1 mRNAの発現量に基づいてスクリーニングされた細胞であるため、既知のがん幹細胞であるALDH1A1に着目し、そのタンパクレベルの発現を免疫染色法を用いて解析した。その結果、高い腫瘍形成能を示した2種類のクローン株において、ALDH1A1の高い発現を確認できた。
    以上のことから、本年度は、樹立したがん幹細胞のモデル細胞のうち、2種類の細胞がin vivoにおいて高い腫瘍形成能を示すこと、これらの細胞では、ALDH1A1ががん幹細胞マーカーとして発現することを明らかにした。今後、これらの細胞が、がん幹細胞への新規薬物送達法を確立するための有用なモデル細胞になると考えらえれた。

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  • グリオーマ癌幹細胞特異的に発現する新規バイオマーカーの機能解析

    2017.04

    基盤研究(C)

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    Grant type:Competitive

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  • Funcional analysis of a novel biomarker specifically expressed in glioma stem cell.

    Grant number:17K07183  2017.04 - 2020.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    Maruyama Masato

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    Grant amount:\5070000 ( Direct expense: \3900000 、 Indirect expense:\1170000 )

    We analyzed the expression of PC3-secreted microseminoprotein (MSMP) in glioma stem cells and indicated that MSMP was expressed in patient-derived glioma stem cell lines and human glioma tissues. These results suggested that MSMP expressed in glioma stem cells migrate peripheral blood monocytes from blood vessele to tumor tissues and contributed to the formation of tumor microenvironment.

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  • グリオーマ癌幹細胞特異的に発現する新規バイオマーカーの機能解析

    2017

    日本学術振興会  基盤研究(C)

    丸山 正人

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\780000 ( Direct expense: \780000 )

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  • グリオーマ癌幹細胞特異的に発現する新規バイオマーカーの機能解析

    2017 - 2019

    日本学術振興会  基盤研究(C)

    丸山 正人

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\1820000 ( Direct expense: \1820000 )

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  • グリオーマ癌幹細胞特異的に発現する新規バイオマーカーの機能解析

    2017 - 2019

    日本学術振興会  基盤研究(C)

    丸山 正人

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\2700000 ( Direct expense: \2700000 )

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  • グリオーマ癌幹細胞特異的に発現する新規バイオマーカーの機能解析

    2017 - 2019

    日本学術振興会  基盤研究(C)

    丸山 正人

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\3300000 ( Direct expense: \3300000 )

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  • Novel therapeutic strategy for glioma by construction of glioma stem cell specific gene expression system.

    Grant number:25460049  2013.04 - 2016.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    MARUYAMA Masato, KASE Masahiko, TRIFONOV Stefan, SAKURAI Fuminori

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    Grant amount:\5070000 ( Direct expense: \3900000 、 Indirect expense:\1170000 )

    Gliomas are most common type of brain tumor and malignant gliomas have high recurrence rates and poor prognosis. In recent years, cancer stem cells are considered to be a cause of metastasis and recurrence after treatment. Since past therapeutic strategy against glioma targets whole tumor cells, these strategy are not effective for cancer stem cells which shows resistant to therapy. Here, we established human glioma stem cell lines and constructed molecular basis to specifically express therapeutic genes in glioma stem cells.

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  • Biochemical and physiological studies on deubiquitinating enzymes based on an analysis of the enzymatic activity dynamics

    Grant number:23310160  2011.04 - 2014.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    HATTORI Akira, OISHI Shinya, FUJIWARA Hiroshi, INOUE Hideshi, MARUYAMA Masato, NISHIMURA Shinichi

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    Grant amount:\20020000 ( Direct expense: \15400000 、 Indirect expense:\4620000 )

    Post-translational modification of cellular proteins by ubiquitin (Ub) is involved in various aspects of cell physiology, such as protein degradation via proteasome, DNA repair, and membrane trafficking. Deubiqutinating enzymes liberate a Ub moiety from polyUb chains attached on substrate proteins. In the current study, we investigated novel research tools for the enzymatic characterization of deubiquitinating enzymes. Ub-granzyme B (Ub-GrB), an N-terminal Ub fusion mature granzyme B was expressed in a baculovirus system. By employing Ub-GrB, we showed that human ubiquitin specific protease 47 is active. In addition, we explored oxidative stress-sensitive deubiquitinating enzymes, and found that ubiquitin C-terminal hydrolase-L3 is susceptible to reactive oxygen species by utilizing a Ub activity-based probe, Ub-vinyl sulfone.

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  • iPS cell-based regeneration therapy for Alzheimer's disease.

    Grant number:22790092  2010 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    MARUYAMA Masato

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    Grant amount:\3380000 ( Direct expense: \2600000 、 Indirect expense:\780000 )

    There is no curative treatment for Alzheimer's diseases characterized by neuronal degeneration. To apply iPS cells to Alzheimer's disease, we established a method for efficient purification of neural stem cells derived from mouse iPS cells.

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  • Elucidation of physiological role of Laeverin, a novel aminopeptidase.

    Grant number:20790088  2008 - 2009

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    MARUYAMA Masato

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    Grant amount:\4290000 ( Direct expense: \3300000 、 Indirect expense:\990000 )

    Here, we evaluated the roles of His^<379>, comprising the exopeptidase motif, in the enzymatic properties of human Laeverin. Our results indicate that His^<379> plays essential roles in its distinctive enzymatic properties and contributes to maintaining the appropriate structure of the catalytic cavity of the enzyme.

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  • Mechanisms of Enzymatic Action of the Oxytocinase Subfamily of Aminopeptidases

    Grant number:18390031  2006 - 2008

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    TSUJIMOTO Masafumi, HATTORI Akira, MARUYAMA Masato, GOTO Yoshikuni

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    Grant amount:\17700000 ( Direct expense: \15300000 、 Indirect expense:\2400000 )

    本研究において私たちは妊娠の維持、記憶の維持、血圧調節、ガン細胞の増殖制御、抗原ペプチドのプロセシングなどその生理的/病理的重要性が明らかとなってきたオキシトシナーゼサブファミリーを含むM1アミノペプチダーゼ酵素の反応を点変異体を用いて解析し、M1酵素の基質特異性を決定している残基を同定することに成功した。これらの成果はM1酵素の反応機構および基質結合部位の構造を解明し、M1酵素を標的する医薬品を開発するうえで重要な知見を与えると考えられる。

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  • アンギオテンシンIV受容体の細胞内ダイナミクスとその分子機構の解明

    2005.04 - 2007.03

    若手研究(B)

    丸山 正人

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\3500000 ( Direct expense: \3500000 )

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  • アンギオテンシンIV受容体の細胞内ダイナミクスとその分子機構の解明

    Grant number:17790080  2005 - 2006

    日本学術振興会  科学研究費助成事業 若手研究(B)  若手研究(B)

    丸山 正人

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    Grant amount:\3500000 ( Direct expense: \3500000 )

    学習・記憶改善作用を示すアンギオテンシン(Ang)IVは、特異的な受容体である胎盤性ロイシンアミノペプチダーゼ(P-LAP)を介して作用を発揮することが知られている。P-LAPは細胞内小胞に存在し、脂肪細胞ではインスリン刺激により細胞膜へと移行することが知られているが、神経細胞での膜移行を誘導する生理的因子は全く不明である。そこで本年度は、膜表面ビオチン化法を用いて神経細胞におけるP-LAPの膜移行を惹起するペプチドホルモンを探索した。その結果、サブスタンスP(SP)で刺激した細胞では、SPの濃度及び時間依存的に細胞膜表面P-LAP量が増加することを明らかにした。脂肪細胞では、インスリン刺激によりP-LAPが細胞膜へと移行するが、SP刺激では細胞膜移行が見られなかったこと、また神経細胞では、インスリン刺激による膜表面P-LAP量の増加が認められなかったことから、見出した現象が神経細胞において特異的な現象であることを明らかにした。また、SPはcAMPをセカンドメッセンジャーとして細胞内にシグナルを伝達することが知られているため、細胞外にcAMPを処理した際のP-LAP膜移行について検討した。その結果、cAMPが細胞膜P-LAP量を有意に上昇させる現象が観察されたため、以上の結果と併せて、SPが細胞内cAMPを介して情報伝達されることにより、AngIV受容体の細胞膜移行を促進させ、膜表面上に増加したAngIV受容体が、AngIVの生理作用を増強する可能性が示された。これらの成果を、第11回病態と治療におけるプロテアーゼとインヒビター研究会(ポスター奨励賞受賞)、第28回生体膜と薬物の相互作用シンポジウム、RIKEN International symposium on chemical biology,において発表した。

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