Updated on 2024/04/06

写真a

 
MIYAZAKI Yuko
 
Organization
Faculty of Environmental, Life, Natural Science and Technology Associate Professor
Position
Associate Professor
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Degree

  • 博士 ( 2009.3   環境科学(北海道大学) )

Research Interests

  • 植物生態学

  • Plant Ecology

Research Areas

  • Life Science / Forest science

  • Life Science / Ecology and environment

Education

  • Hokkaido University   大学院農学研究科   環境資源学専攻 博士後期課程

    2002.4 - 2003.3

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  • Hokkaido University   大学院農学研究科   環境資源学専攻 博士前期課程

    2000.4 - 2002.3

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    Country: Japan

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  • Hokkaido University    

    - 2000

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  • Hokkaido University    

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Research History

  • Okayama University   学術研究院環境生命科学学域   Associate Professor

    2021.4

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  • Okayama University   The Graduate School of Environmental and Life Science   Associate Professor

    2020.2 - 2021.3

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    Country:Japan

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  • Okayama University   Graduate School of Environmental and Life Science   Assistant Professor

    2012.4 - 2020.1

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  • Okayama University   大学院環境学研究科   Assistant Professor

    2011.4 - 2012.3

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  • 北海道大学大学院 地球環境科学研究科   博士研究員

    2011.1 - 2011.3

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  • Hokkaido University Creative Research Institution   Researcher

    2009.4 - 2010.12

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  • Nara Forest Research Institute

    2003.4 - 2009.3

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Professional Memberships

Committee Memberships

  •   岡山県環境影響評価技術審査委員会委員  

    2023.4   

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    Committee type:Municipal

  •   岡山県環境影響評価技術審査委員会委員  

    2023.4   

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    Committee type:Municipal

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  •   岡山県国土利用計画審議会委員  

    2023.2   

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    Committee type:Municipal

  •   岡山県国土利用計画審議会委員  

    2023.2   

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    Committee type:Municipal

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  •   岡山市自然環境保全審議会委員  

    2019.4   

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    Committee type:Municipal

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Papers

  • A malformation of sex-changing plant Arisaema serratum (Araceae) produces both male and female inflorescences Reviewed

    Tetsuya K. Matsumoto, Ryohei Fujisato, Munetaka Sugiyama, Yuko Miyazaki, Jin Murata

    BOTANY LETTERS   2023.7

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:TAYLOR & FRANCIS LTD  

    The genus Arisaema (Araceae) can change sex expression between male and female with increasing plant size (sequential hermaphrodism). As most Arisaema species have only one inflorescence on each individual, sex expression is seemingly determined at the individual level. However, malformations with male and female inflorescences have occasionally been observed. Unfortunately, their developmental origin is unclear because of a lack of detailed descriptions of the shoot structure. To estimate the unit of sex determination in Arisaema, we observed the morphology of an aberrant individual of Arisaema serratum (Thunberg) Schott with multiple inflorescences for three years. In the second year, the aberrant individual produced pure male and female inflorescences. Based on the phyllotactic pattern of this malformation, we estimated that the inflorescences were duplicated due to a broadening of the apical meristem via fasciation. Contrary to other Arisaema plants with multiple inflorescences reported previously, the corm of the aberrant individual did not subdivide nor branch spontaneously after the growing season for three years. Thus, duplicated inflorescences presumably shared the resource supply without physiological partitioning during sex determination. These results imply that sex expression is determined at the inflorescence level but not at the individual or floret levels in dioecious Arisaema.

    DOI: 10.1080/23818107.2023.2234973

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  • Two closely related species of the Arisaema ovale group (Araceae) selectively attract male fungus gnats of different Anatella species (Diptera: Mycetophilidae) Reviewed

    Tetsuya K. Matsumoto, Masahiro Sueyoshi, Shigetaka Sakata, Yuko Miyazaki, Muneto Hirobe

    Plant Systematics and Evolution   309   4   2023.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s00606-022-01839-7

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  • Gall midge pollination and ant-mediated fruit dispersal of Pinellia tripartita (Araceae)

    Tetsuya K. Matsumoto, Motoya Onoue, Takashi Miyake, Kentaro Ohnishi, Kiyoto Takazoe, Muneto Hirobe, Yuko Miyazaki

    Plant Ecology   2022.12

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    Authorship:Last author   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s11258-022-01278-x

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    Other Link: https://link.springer.com/article/10.1007/s11258-022-01278-x/fulltext.html

  • Evaluating the soil microbe community‐level physiological profile using <scp>EcoPlate</scp> and soil properties at 33 forest sites across Japan

    Masahiro Nakamura, Chisato Terada, Kinya Ito, Kazuaki Matsui, Shigeru Niwa, Masae Ishihara, Tanaka Kenta, Tetsuro Yoshikawa, Taku Kadoya, Tsutom Hiura, Hiroyuki Muraoka, Ken Ishida, Naoki Agetsuma, Ryosuke Nakamura, Hitoshi Sakio, Masahiro Takagi, Akira S. Mori, Megumi K. Kimura, Hiroko Kurokawa, Tsutomu Enoki, Tatsuyuki Seino, Atsushi Takashima, Hajime Kobayashi, Kazuho Matsumoto, Koichi Takahashi, Ryunosuke Tateno, Tomohiro Yoshida, Tatsuro Nakaji, Masayuki Maki, Kazutaka Kobayashi, Karibu Fukuzawa, Kazuhiko Hoshizaki, Kazuhide Ohta, Keito Kobayashi, Motohiro Hasegawa, Satoshi N. Suzuki, Michinori Sakimoto, Yoichiro Kitagawa, Akiko Sakai, Hirofumi Kondo, Tomoaki Ichie, Koji Kageyama, Ayaka Hieno, Shogo Kato, Tatsuya Otani, Yasuhiro Utsumi, Tomonori Kume, Kosuke Homma, Koju Kishimoto, Kazuhiko Masaka, Kenta Watanabe, Motomu Toda, Dai Nagamatsu, Yuko Miyazaki, Tamon Yamashita, Naoko Tokuchi

    Ecological Research   37 ( 3 )   432 - 445   2022.2

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    We present the largest freely available EcoPlate dataset for Japan, comprising data collected from a network of 33 natural forest sites (77 plots) in regions of East Asia ranging from cool temperate to subtropical. EcoPlate is a 96-well microplate that contains three repeated sets of 31 response wells with different sole carbon substrates. The utilization of each carbon substrate by the microbial community is quantified by the color density of the well during incubation. EcoPlate can provide a multifunctional index of a soil microbial community. Soil properties (water content, carbon [C] and nitrogen [N] contents, the C/N ratio, and pH) that are essential for interpreting the EcoPlate results were also measured. The network is part of the Forest and Grassland Survey of the Monitoring Sites 1000 Project established by the Ministry of the Environment. Using a standardized protocol, soil was sampled between September and December 2020. A preliminary principal component analysis was performed on the temporal integration of color density using 31 substrates. For the temporal integration, we calculated the cumulative amount of color development by integrating the color density development curve. PC1 contributed 55.6% and is thought to represent the magnitude of the overall absorbance of all substrates. The model of environmental factors including elevation and the model of soil properties, including water content and pH, were selected as the best-fit models for variation in PC1. EcoPlate data enable the meta-analysis of comparative studies among forest types and testing community ecology and ecosystem function hypotheses at broad spatial scales. The complete data set for this abstract published in the Data Paper section of the journal is available in electronic format in MetaCat in JaLTER at http://db.cger.nies.go.jp/JaLTER/metacat/metacat/ERDP-2022-01.1/jalter-en.

    DOI: 10.1111/1440-1703.12293

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  • Selective pollination by fungus gnats potentially functions as an alternative reproductive isolation among five Arisaema species. International journal

    Tetsuya K Matsumoto, Muneto Hirobe, Masahiro Sueyoshi, Yuko Miyazaki

    Annals of botany   2020.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND AND AIMS: Interspecific difference in pollinators (pollinator isolation) is important for reproductive isolation in flowering plants. Species-specific pollination by fungus gnats has been discovered in several plant taxa, suggesting that they can contribute to reproductive isolation. Nevertheless, their contribution has not been studied in detail, partly because they are too small for field observations during flower visitation. To quantify their flower visitation, we used the genus Arisaema (Araceae) because the pitcher-like spathe of Arisaema can trap all floral visitors. METHODS: We evaluated floral visitor assemblage in an altitudinal gradient including five Arisaema species. We also examined interspecific differences in altitudinal distribution (geographic isolation) and flowering phenology (phenological isolation). To exclude the effect of interspecific differences in altitudinal distribution on floral visitor assemblage, we established 10 experimental plots including the five Arisaema species on high- and low-altitude areas and collected floral visitors. We also collected floral visitors in three additional sites. Finally, we estimated the strength and contribution of these three reproductive barriers using the unified formula for reproductive isolation. KEY RESULTS: Each Arisaema species selectively attracted different fungus gnats in the altitudinal gradient, experimental plots, and additional sites. Altitudinal distribution and flowering phenology differed among the five Arisaema species, whereas the strength of geographic and phenological isolations were distinctly weaker than those in pollinator isolation. Nevertheless, the absolute contribution of pollinator isolation to total reproductive isolation was weaker than geographic and phenological isolations, because pollinator isolation functions after the two early-acting barriers in plant life history. CONCLUSIONS: Our results suggest that selective pollination by fungus gnats potentially contributes to reproductive isolation. Since geographic and phenological isolations can be disrupted by habitat disturbance and interannual climate change, the strong and stable pollinator isolation might compensate for the weakened early-acting barriers as an alternative reproductive isolation among the five Arisaema species.

    DOI: 10.1093/aob/mcaa204

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  • Predicting effects of climate change on productivity and persistence of forest trees Reviewed

    Russell D. Kramer, H. Roaki Ishii, Kelsey R. Carter, Yuko Miyazaki, Molly A. Cavaleri, Masatake G. Araki, Wakana A. Azuma, Yuta Inoue, Chinatsu Hara

    ECOLOGICAL RESEARCH   35 ( 4 )   562 - 574   2020.7

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY  

    Global climate change increases uncertainty in sustained functioning of forest ecosystems. Forest canopies are a key link between terrestrial ecosystems, the atmosphere, and climate. Here, we introduce research presented at the 66th meeting of the Ecological Society of Japan in the symposium "Structure and function of forest canopies under climate change." Old-growth forest carbon stores are the largest and may be the most vulnerable to climate change as the balance between sequestration and emission could easily be tipped. Detailed structural analysis of individual large, old trees shows they are allocating wood to the trunk and crown in patterns that cannot be deduced from ground, thus can be used to more accurately quantify total forest carbon and sequestration. Slowly migrating species sensitive to novel climatic conditions will have to acclimate at the individual level. Accounting for physiological responses of trees to climate change will improve predictions of future species distributions and subsequent functioning of forest ecosystems. Field experiments manipulating temperature and precipitation show how trees compensate physiologically to mitigate for higher temperatures and drought. However, it is difficult to measure acclimation responses over long timeframes. Intraindividual trait variation is proposed as an indicator of acclimation potential of trees to future conditions and suggests that acclimation potential may vary among regional populations within a species. Integrating whole-tree structural data with physiological data offers a promising avenue for understanding how trees will respond to climatic shifts.

    DOI: 10.1111/1440-1703.12127

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  • Corrigendum: Development of microsatellite markers for the annual andromonoecious herb Commelina communis f. ciliata (Commelinaceae) [Genes Genet. Syst. (2019) 94, p. 133–138]

    Koki R. Katsuhara, Naoyuki Nakahama, Taketo Komura, Masaya Kato, Yuko Miyazaki, Yuji Isagi, Motomi Ito, Atushi Ushimaru

    Genes & Genetic Systems   95 ( 1 )   51 - 51   2020.2

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    Publishing type:Research paper (scientific journal)   Publisher:Genetics Society of Japan  

    DOI: 10.1266/ggs.95.51

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  • Pre-pollination barriers between two sympatric Arisaema species in northern Shikoku Island, Japan Reviewed

    Tetsuya K. Matsumoto, Yuko Miyazaki, Masahiro Sueyoshi, Yoshihiro Senda, Kazuhiro Yamada, Muneto Hirobe

    AMERICAN JOURNAL OF BOTANY   2019.11

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY  

    Premise The genus Arisaema (Araceae) has rapidly diversified in Japan, and multiple species often coexist in the field. Although Japanese Arisaema species hybridize from artificial crossing, hybrid individuals are rare in mixed populations; suggesting the presence of effective pre-pollination barriers. We examined the following reproductive barriers between A. sikokianum and A. tosaense: habitat, phenology, and pollinator isolations. Methods Habitat isolation was examined by interspecific comparisons of microhabitat conditions at a mixed site and of altitude at the sampling site of herbarium specimens. Phenological isolation was evaluated by comparing seasonal transition in apparent spathe condition and frequency of insect visitation. Pollinator isolation was examined by comparing floral visitor assemblages between the two Arisaema species. To avoid overestimation of pollinator isolation due to seasonal changes in insect assemblages, we also compared visitor assemblages between natural and late-flowering A. sikokianum, where the latter was experimentally introduced and blooming with a natural A. tosaense population. Results Microhabitat conditions and sampling elevations of herbarium specimens overlapped between the two Arisaema species. At the population level, A. sikokianum and A. tosaense flowered for 39 and 52 days, respectively, with 13 days overlap. Insect visitation in A. sikokianum decreased before the seasonal overlap. Floral visitor assemblages differed between the two Arisaema species, while the difference between natural and late-flowering A. sikokianum was less distinct. Conclusions Phenological and pollinator isolation contribute to reproductive isolation between the two Arisaema species and should enable the two species to coexist in this area.

    DOI: 10.1002/ajb2.1389

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  • Development of microsatellite markers for the annual andromonoecious herb Commelina communis f. ciliata (Commelinaceae) Reviewed

    Koki R. Katsuhara, Naoyuki Nakahama, Taketo Komura, Masaya Kato, Yuko Miyazaki, Yuji Isagi, Motomi Ito, Atushi Ushimaru

    GENES & GENETIC SYSTEMS   94 ( 3 )   133 - 138   2019.6

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:GENETICS SOC JAPAN  

    Commelina communis f. ciliata (Commelinaceae), a newly distinguished taxon, is an annual andromonoecious herb exhibiting a mixed mating system, the details of which remain unclear. We developed microsatellite markers for use in exploring the evolution of andromonoecy and mixed mating in the species. Fifteen micro satellite loci were developed using next-generation sequencing. The primer sets were used to evaluate 65 C. communis f. ciliata individuals from three populations in Japan; we found 1-13 alleles per locus and the expected heterozygosity ranged from 0.00 to 0.76. The markers are potentially useful to examine intra- and inter-species genetic structure and the mixed mating strategy of Commelina species via paternity analysis.

    DOI: 10.1266/ggs.18-00058

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  • Population structures and spatial patterns of two unpalatable Arisaema species (Araceae) with and without clonal reproduction in riparian forest intensively grazed by Sika deer Reviewed

    Matsumoto TK, Hirobe M, Akaji Y, Miyazaki Y

    Journal of Forestry Research   2019

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  • The opportunities and challenges of applying tree climbing techniques as a canopy access method in canopy biology Reviewed

    Nakanishi Akira, Azuma Wakana, Tanaka Mizue, Miyazaki Yuko, Inui Yoko

    JAPANESE JOURNAL OF ECOLOGY   68 ( 2 )   125 - 139   2018

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    Language:Japanese   Publisher:The Ecological Society of Japan  

    Canopy biology is the natural science that aimed at understanding of the biodiversity, biological processes, and ecological functions of forest canopies. Canopies determine the structural and energetic properties of forest ecosystems. Since the 1980s, canopy biology has progressed rapidly through the development of methods for accessing treetops. The rope climbing techniques used widely in canopy studies have developed from diverse procedures that allow human access to the top layers of forest vegetation. In comparison with other access hardware, e.g., cranes and gantries, rope assemblies have advantages in terms of user mobility, repeated access, and cost. The availability and safety of tree climbing techniques have improved with recent developments in mountain climbing gear and methodologies for their use. In this review, we use candidate studies to introduce the advantages, prospects and challenges of climbing techniques for tree canopy studies. Tree climbing allows excellent access to treetops in all types of forests, across all geographical locations. We expect further progress through combinations of rope climbing and other access methodologies. In the interests of safety and effectiveness, a platform should be developed for the distribution of relevant information to prospective tree climbing researchers and those who may wish to use the procedures for other activities, such as arboriculture.

    DOI: 10.18960/seitai.68.2_125

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    Other Link: https://agriknowledge.affrc.go.jp/RN/2010922617

  • Relationship between seasonal progression of floral meristem development and FLOWERING LOCUS T expression in the deciduous tree Fagus crenata Reviewed

    Yuko Miyazaki, Akiko Satake

    ECOLOGICAL RESEARCH   32 ( 4 )   627 - 631   2017.7

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER JAPAN KK  

    Estimating the timing of flower bud formation in plants is essential to identify environmental factors that regulate floral transition. The presence of winter dormancy between the initiation of flowers and anthesis, characteristic of most trees in the temperate forests, hampers accurate estimation of the timing of floral transition. To overcome this difficulty, expression levels of flowering-time genes could be used as indicators of the timing of floral transition. Here, we evaluated the usefulness of molecular markers in estimating the timing of floral transition in Fagus crenata, a deciduous tree that shows intermittent and synchronized flowering at the population level. We selected FLOWERING LOCUS T (FT) as a candidate molecular marker and quantified the expression levels of its ortholog in F. crenata (FcFT). Subsequently, we analyzed the relationship between morphogenetic changes that occur between the vegetative state of the buds and the initiation of floral organs, and compared the FcFT expression levels in reproductive and vegetative buds, collected from spring to autumn. FcFT expression in leaves peaked at least two weeks before the morphological changes associated with flowering were visible in the buds in late July. FcFT expression levels were significantly higher in the reproductive buds than in the vegetative buds in July. These results suggest that the FcFT expression in July is a reliable indicator of the timing and occurrence of floral transition. This study highlights the utility of molecular tools in unraveling reproductive dynamics in plants, in combination with ecological and physiological approaches.

    DOI: 10.1007/s11284-017-1462-3

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  • Survival and growth of Fagus crenata seedlings in relation to biological and microtopographical factors in a cool temperate broadleaf forest Reviewed

    Yasuaki Akaji, Muneto Hirobe, Yuko Miyazaki, Takushi Makimoto, Shu Kinoshita, Itsuka Hattori, Keiji Sakamoto

    JOURNAL OF FOREST RESEARCH   22 ( 5 )   294 - 302   2017

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:TAYLOR & FRANCIS LTD  

    The seedling stage is critical in the life cycle of trees, and performance is controlled by both abiotic and biotic factors, which are spatially heterogeneous. We investigated the relationships between the seedling survival or growth of Fagus crenata and seven potentially influencing factors (culm density of dwarf bamboo, slope angle, topographic convexity, distance from the nearest conspecific adult, and local density of F. crenata seedlings, and seedling size and age), under consideration of the changes in direction and strength of the microtopographic effects across space. In 2011, we identified all F. crenata seedlings (maximum stem length &lt;50cm) within a 90x30-m plot in a cool-temperate forest in western Japan and then recorded their survival and growth of stem length in 2012. The best model based on Akaike's information criterion indicated that the size, age, and distance had positive effects on the survival while slope and convexity had negative effects, and that the strength of the negative effect of convexity varied spatially even within our plot. Meanwhile, the size and culm density had positive and negative effects on the growth, respectively. These results suggest that F. crenata seedlings have more chance to survive at the site on a gentle slope with planar or relatively concave undulations, where the conspecific adults stood apart, and that the seedlings show higher growth rate at the sites without dense dwarf bamboo. Furthermore, we demonstrate that the spatially nonstationary effect of microtopography controls the spatial pattern of seedling survival.

    DOI: 10.1080/13416979.2017.1354749

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  • Survival and growth of Fagus crenata seedlings in relation to biological and microtopographical factors in a cool temperate broadleaf forest (vol 22, pg 294, 2017)

    Y. Akaji, M. Hirobe, Y. Miyazaki, T. Makimoto, S. Kinoshita, Hattori, I, K. Sakamoto

    JOURNAL OF FOREST RESEARCH   22 ( 5 )   III - III   2017

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    Language:English   Publisher:TAYLOR & FRANCIS LTD  

    DOI: 10.1080/13416979.2017.1362154

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  • The relationship between seedling survival rates and their genetic relatedness to neighboring conspecific adults Reviewed

    Yasuaki Akaji, Yuko Miyazaki, Muneto Hirobe, Takushi Makimoto, Keiji Sakamoto

    PLANT ECOLOGY   217 ( 4 )   465 - 470   2016.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER  

    Seedling genotype is one of the major factors affecting seedling survival when the rate of damage by natural enemies depends on a host genotype. However, to our knowledge, no previous study has examined the mortality of seedlings adjacent to conspecific adults, considering intraspecific variation in seedling genotypes. On the basis of the assumption that natural enemies adapt to adult trees having a unique genotype, we tested the hypothesis that seedling survival decreases when they are more closely genetically related to neighboring adults, by measuring the mortality rate of seedlings of Fagus crenata growing in a cool-temperate forest in Japan. We estimated the genetic relatedness of seedlings (2 and 6 years old) to the neighboring adult F. crenata using microsatellite analysis and measured seedling survival. We determined that there was a non-significant negative correlation between seedling survival and genetic relatedness in the 2-year-old seedling cohort, and a non-significant but positive correlation in the 6-year-old seedlings. Our results call for further studies on the potential relationship between seedling survival and genetic relatedness to neighboring adults.

    DOI: 10.1007/s11258-016-0591-z

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  • Temporal and spatial dynamics of an old-growth beech forest in western Japan Reviewed

    Uyanga Ariya, Ken-Ya Hamano, Takushi Makimoto, Shu Kinoshita, Yasuaki Akaji, Yuko Miyazaki, Muneto Hirobe, Keiji Sakamoto

    JOURNAL OF FOREST RESEARCH   21 ( 2 )   73 - 83   2016.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:TAYLOR & FRANCIS LTD  

    Dendrochronological approaches enable us to understand forest stand dynamics by estimation of disturbance history and age structure. The present study was conducted in an old-growth beech forest in a forest reserve in western Japan. Increment cores were taken for tree ring analysis from all canopy trees in a 50 m x 130 m study plot. Radial growth release criteria were developed to identify significant growth releases in each tree ring series and to characterize the disturbance history of the study site. The age structure of the forest was indicative of continuous establishment by Fagus crenata and simultaneous establishment by Magnolia obovata. A variety of low-intensity disturbances were identified in each decade, especially after the 1900s, but the occurrence of high-intensity catastrophic disturbance was rare, and likely played an important role in maintaining species diversity in the existing forest canopy. The results also suggest that F. crenata regenerates gradually before and after both large- and small-scale disturbances, whereas M. obovata and Betula grossa regenerate only after large-scale catastrophic disturbances.

    DOI: 10.1007/s10310-015-0514-1

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  • Planning a management program for expanding bamboo forests based on scenario analysis Reviewed

    Miyazaki Y, Osawa T, Mitsuhashi H

    Japanese Journal of Conservation Ecology   20 ( 1 )   3 - 14   2015

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:The Ecological Society of Japan  

    To manage the expansion of bamboo forests efficiently, it is necessary to predict where expansion is likely. However, predicting bamboo distribution using statistical models is challenging due to biased distributions, heterogeneous invasion histories, and strong neighbor effects. Furthermore, clonal and invasive species such as bamboo show a non-linear response to environmental factors. Here, we construct a spatial model to predict locations of high penetration probability after controlling for these problems, using data for bamboo distribution in Toyooka, Hyogo Prefecture, Japan. First, we used MaxEnt to reveal the full range of possible bamboo survival and to control for the effects of clonal growth. Second, we constructed a predictive model of invasion using environmental factors. The first model showed that distance from the source patch strongly affected expansion and that constraints from environmental factors were not significant. The final model explained about 70% of actual expansion. Scenario analyses showed that bamboo expansion is best controlled by removing smaller patches. Based on our results, we discuss issues in bamboo forest management and planning.

    DOI: 10.18960/hozen.20.1_3

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  • Nitrogen as a key regulator of flowering in Fagus crenata: understanding the physiological mechanism of masting by gene expression analysis Reviewed

    Yuko Miyazaki, Yosuke Maruyama, Yukako Chiba, Masaki J. Kobayashi, Benesh Joseph, Kentaro K. Shimizu, Keiichi Mochida, Tsutom Hiura, Hirokazu Kon, Akiko Satake

    ECOLOGY LETTERS   17 ( 10 )   1299 - 1309   2014.10

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-BLACKWELL  

    The role of resource availability in determining the incidence of masting has been widely studied, but how floral transition and initiation are regulated by the resource level is unclear. We tested the hypothesis that floral transition is stimulated by high resource availabiltiy in Fagus crenata based on a new technique, the expression analyses of flowering genes. We isolated F. crenata orthologues of FLOWERING LOCUS T, LEAFY and APETALA1, and confirmed their functions using transgenic Arabidopsis thaliana. We monitored the gene expression levels for 5 years and detected a cycle of on and off years, which was correlated with fluctuations of the shoot-nitrogen concentration. Nitrogen fertilisation resulted in the significantly higher expression of flowering genes than the control, where all of the fertilised trees flowered, whereas the control did not. Our findings identified nitrogen as a key regulator of mast flowering, thereby providing new empirical evidence to support the resource budget model.

    DOI: 10.1111/ele.12338

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  • Dynamics of internal carbon resources during masting behavior in trees Reviewed

    Yuko Miyazaki

    ECOLOGICAL RESEARCH   28 ( 2 )   143 - 150   2013.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER JAPAN KK  

    Several proximate factors of masting have been provided. Here, I focus on the role of internal factors, especially the relationship between internal carbon resources and modular structures in trees. I summarize various studies of carbon resource allocation for reproduction during masting events in terms of the proximate factors of masting and discuss the modular structure in which trees accumulate and consume carbon resources as well as the timing when internal carbon resources affect masting since trees have complex resource dynamics among organs. The resource budget model, which provides a simple mechanistic explanation of the masting mechanism, is supported by various study lines. This model assumes decreasing levels of stored photosynthate after flowering and fruiting. According to several studies, however, carbon reserves do not decrease after fruiting in species in which the modules autonomously allocate current photosynthate for fruiting. In addition, it is important to elucidate when carbon resources affect masting events because during their long developmental processes, trees pass through various stages until they produce maturing fruits to create successful masting events. To explore the mechanisms of masting in future studies, it would be important to figure out how and when candidate factors (including nutrients other than carbon) may influence the entire reproduction process, for example, using field manipulation experiments.

    DOI: 10.1007/s11284-011-0892-6

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  • Dwarf bamboos (Sasa pubiculmis Makino subsp. pubiculmis) are not monocarpic plants : evidence from flowering pattern and genetic structure(<Feature>Why bamboos show gregarious flowering habits? -with special reference to the evolutional tren Reviewed

    Miyazaki Yuko, Ohnishi Naoki, Hino Takafumi, Hiura Tsutom

    JAPANESE JOURNAL OF ECOLOGY   60 ( 1 )   73 - 79   2010

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    Language:Japanese   Publisher:日本生態学会暫定事務局  

    ササ類は開花・結実の豊凶がきわめて明瞭であり、加えて生涯で一度のみ繁殖する一回繁殖型植物と考えられてきた。一方、ササ類は個体群の遺伝構造の把握が困難であったため、これまで一回繁殖性について検証されることはなかった。しかしササ類の特異的な繁殖特性を解明するにはまず一回繁殖性の検証が不可欠である。そこで、オモエザサ個体群において遺伝構造を調べると共に、4年間の開花パターンの調査を行い、一回繁殖性の検証を行った。その結果、開花稈は全て同一ジェネットに属し、ジェネットの占有面積は3ha以上に及んだ。しかし、同一ジェネット内の稈が全て開花したのではなく、大別すると、全ての稈が開花していない範囲、開花稈と非開花稈が混在している範囲、全ての稈が開花している範囲がそれぞれ、同一ジェネット内に同時に存在した。全ての稈が開花した範囲のうち、開花後に全ての地上部が枯死した範囲もあったが、翌年以降も稈が生残する範囲もあった。開花後に地上部が枯死した範囲の地下茎からは、翌年以降に新しい稈や花序が再生する現象がみられた。開花が起こる範囲は年毎に変化したが、同一ジェネットに属していても4年間でまだ一度も開花していない範囲も存在した。また、ラメット間の生理的統合を調べるため、炭素安定同位体(&lt;sup&gt;13&lt;/sup&gt;C)を用いてトレース実験を行った結果、非開花稈から地下茎で繋がった開花稈へ&lt;sup&gt;13&lt;/sup&gt;Cが転流していた。これらのことから、研究対象としたオモエザサは一斉に開花はするものの、必ずしも一回繁殖型の生活史を有していないことが分かった。

    DOI: 10.18960/seitai.60.1_73

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  • Resource level as a proximate factor influencing fluctuations in male flower production in Cryptomeria japonica D. Don Reviewed

    Yuko Miyazaki, Takeshi Osawa, Yoshiaki Waguchi

    JOURNAL OF FOREST RESEARCH   14 ( 6 )   358 - 364   2009.12

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    Airborne pollen of Cryptomeria japonica causes severe pollinosis, and the number of people suffering from pollinosis has increased rapidly during recent decades. Male flower production in C. japonica shows annual variability; thus, predicting male flower production for the following spring could be a countermeasure against pollinosis. We analyzed factors that explain fluctuations in male flower production of C. japonica according to parameters reflecting internal resource dynamics based on field observations. In addition, we carried out experiments to regulate available resource levels using artificial shading to test the effect of internal resource levels on the number of male flowers produced. We found that temperature and precipitation in the previous summer and a relative increase in male flower production in the previous year were negatively related, whereas sunshine duration in the previous summer and diameter at breast height were positively related to the current year increase in male flower production. The number of male flowers was significantly reduced by the shading treatment and was positively correlated with the amount of total non-structural carbohydrates in the root. These results suggest that understanding the parameters controlling internal resource levels could lead to more accurate predictions of male flower production.

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  • Genets of dwarf bamboo do not die after one flowering event: evidence from genetic structure and flowering pattern Reviewed

    Yuko Miyazaki, Naoki Ohnishi, Hino Takafumi, Tsutom Hiura

    JOURNAL OF PLANT RESEARCH   122 ( 5 )   523 - 528   2009.9

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    Dwarf bamboos in the genus Sasa are believed to be long-lived, synchronously flowering, and monocarpic plants. However, the monocarpy of dwarf bamboo has not been confirmed, because whether all ramets within one genet flower at the same time cannot be determined without differentiating the genetic structure among ramets. This study aims to evaluate the reproductive traits of Sasa pubiculmis by verifying the monocarpy and physiological integration between flowering ramets and non-flowering ramets during a 4-year flowering period. One genotypically identified genet, which covered an area of approximately 3 ha, had both flowering and non-flowering patches of ramets during the 4-year flowering period (2004-2007). A fraction of the flowering genet remained non-flowering during the 4 years of observation, and did not die after mass flowering. Flowering ramets were physically connected to non-flowering ramets via rhizomes, and assimilated (13)C was allocated from non-flowering ramets to flowering ramets. Consequently, we clarified that this dwarf bamboo potentially has polycarpic reproductive traits rather than monocarpic, and a genet can keep rhizomes and non-flowering patches alive to sustain the organism after mass flowering.

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  • Development and characterization of polymorphic microsatellite DNA markers for Sasa senanensis (Poaceae: Bambuseae) Reviewed

    Yuko Miyazaki, Naoki Ohnishi, Kimiko Hirayama, Junco Nagata

    CONSERVATION GENETICS   10 ( 3 )   585 - 587   2009.6

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    Sasa senanensis is a dwarf bamboo species distributed on the floors of cool temperate forests in Japan and adjacent regions. We isolated eight polymorphic microsatellite loci from this species. The number of alleles ranged from two to eight and the observed heterozygosity per locus from 0.13 to 0.74. Seven of the eight loci were also polymorphic in Sasa nipponica. Most of these markers were successfully amplified in other dwarf bamboo species. These markers will be useful for investigating clonal structure and population genetics in some dwarf bamboo species.

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  • Phylogeographical analysis by genotyping chloroplast DNA in Zelkova serrata

    Fukatsu Eitaro, Shimada Hiromasa, Takii Tadato, Kabemura Yuji, Ubukata Masatoshi, Takahashi Makoto, Nakada Ryogo, Yano Keisuke, Watanabe Atsushi, Koyama Yasuhiro, Odani Jiro, Saito Maki, Miyazaki Yuko

    The Japanese Forest Society Congress   119   40 - 40   2008

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    DOI: 10.11519/jfsc.119.0.40.0

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  • Allocation of photo-assimilated C-13 from reproductive and non-reproductive shoots to fruits in Styrax obassia Reviewed

    Yuko Miyazaki, Tsutom Hiura, Ryo Funada

    PLANT SPECIES BIOLOGY   22 ( 1 )   53 - 57   2007.4

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    DOI: 10.1111/j.1442-1984.2007.00176.x

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  • Allocation of resources to reproduction in Styrax obassia in a masting year Reviewed

    Y Miyazaki, T Hiura, E Kato, R Funada

    ANNALS OF BOTANY   89 ( 6 )   767 - 772   2002.6

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    An analysis is presented of three possible pathways of reproductive allocation, namely, allocation of resources to reproductive organs from reproductive shoots, from non-reproductive shoots and from the main trunk. These pathways were examined by comparing the amount of storage starch in reproductive shoots, non-reproductive shoots and the main trunk in Styrax obassia, a typical masting tree species, during a year of little flowering (1999) and in a mass-flowering year (2000), In addition, we measured rates of light-saturated photosynthesis in leaves of reproductive and non-reproductive shoots to examine the contribution of photosynthetic production to reproductive costs. In both the main trunk and non-reproductive shoots the pattern of seasonal variation in the amount of starch did not differ between 1999 and 2000. However, in the mass-flowering year, the amount of starch in the reproductive shoots was less than that in non-reproductive shoots during the growing season. Thus, reproductive shoots bore most of the cost of reproduction, although non-reproductive shoots and the main trunk also bore some of the cost. Mass-based rates of light-saturated photosynthesis of the leaves of reproductive shoots were significantly higher than those of non-reproductive shoots during both the flowering and the fruiting period. However, leaves of reproductive shoots had a significantly smaller area, a lower mass per area, and lower concentrations of nitrogen than leaves of non-reproductive shoots, although the number of leaves did not differ between the two types of shoots. Therefore, the amount of photosynthate per shoot was significantly lower in reproductive shoots than in non-reproductive shoots. These results suggest that the cost of reproduction depends predominantly on storage starch in reproductive shoots, although it is still unclear how much photosynthate is allocated to reproductive organs from non-reproductive shoots. (C) 2002 Annals of Botany Company.

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  • 成長特性の異なるスギ品種間での樹幹加温に対する発現変動遺伝子解析

    宮崎祐子, 東若菜, UCHIYAMA Christmas, 高木正博, 石井弘明

    日本森林学会大会学術講演集   134th   2023

  • ブナの花が咲くまで (特集 北限のブナ)

    宮崎 祐子, 佐竹 暁子

    北方林業 = Northern forestry, Japan   69 ( 2 )   69 - 72   2018

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  • 岡山県西粟倉村・若杉天然林

    宮崎 祐子, 赤路 康朗

    森林科学   73 ( 0 )   28 - 29   2015

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    DOI: 10.11519/jjsk.73.0_28

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  • 大和川東部流域における竹林の拡大実態とバイオマス資源としての可能性調査

    宮崎 祐子, 和口 美明

    奈良県森林技術センター研究報告   ( 38 )   61 - 63   2009.3

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    大和川東部地域3市(旧奈良市、天理市、桜井市)について、竹林の拡大実態調査を行った。調査にはオルソ化されたデジタル空中写真と1979年(現状把握に用いた写真撮影年の約20年前)の森林簿を用いた。その結果、大和川東部流域3市における竹林面積は過去約20年間で平均して約2.3倍に拡大していた。本数間伐率と稈(かん;地上部を指す)の再生量の関係を見るため、35%の間伐を行ったプロットでは、翌年には間伐前の密度の93%まで再生し、1年間の稈の炭素固定量は、40年生スギ樹幹の年間平均炭素固定量を上回ることが分かった。また、大和川東部流域3市の竹林面積の10%が持続的に利用可能であると仮定すると、年間5159.9tの竹をバイオマス資源として供給することが可能であると試算された。

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  • 奈良県の里山林におけるオサムシ科甲虫相調査

    宮崎 祐子, 染川 知之, 和口 美明

    奈良県森林技術センター研究報告   ( 38 )   65 - 68   2009.3

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    温帯域を中心に世界各地で環境指標生物として注目されているオサムシ科甲虫について、ピットフォールトラップを用いて調査した。4林分で調査を行った結果、4林分で共通して見られたのはオオクロツヤヒラタゴミムシ1種であった。また、樹木の種数が多い林分では、捕獲されたオサムシ科甲虫の種数・個体数が多くなる傾向が見られ、モウソウチクやマダケの優先する林分では種数・個体数が少なくなる傾向が見られた。

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  • 「紀伊半島におけるケヤキ人工林育成マニュアル」の紹介

    宮崎 祐子

    山林   ( 1495 )   43 - 49   2008.12

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  • 葉緑体SNPによるブナの系統地理学的の研究-分布域全体をほぼ網羅したハプロタイプ地図の作成-

    高橋誠, 原正利, 藤井紀行, 陶山佳久, 津田吉晃, 小山泰弘, 片井秀幸, 小谷二郎, 斎藤真己, 上野満, 伊藤聡, 小山浩正, 西川浩己, 小澤創, 宮崎祐子, 瀧井忠人, 和田覚, 島田博匡, 花岡創, 吉丸博志, 松本麻子, 渡邉敦史, 武津英太郎, 岩泉正和, 福田陽子, 橋本光司, 戸丸信弘

    日本生態学会大会講演要旨集   55th   2008

  • ユーカリ材の強度性能

    中田 欣作, 宮崎 祐子

    奈良県森林技術センター研究報告   ( 36 )   13 - 17   2007.3

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    ブラジルパラナ州北部、南部およびウルグアイ産のユーカリ材の強度試験および耐朽性試験を行った。ユーカリ材の密度は0.44~1.05g/cm2と分布範囲が大きく、産地の違いによる密度の違いが認められた。ユーカリ材のヤング係数、曲げ強さ、衝撃曲げ吸収エネルギーおよび収縮率は密度の増加とともに増大した。パラナ州北部産のユーカリ材は、密度が0.93g/cm2と最も高く、ヤング係数が22.2kN/mm2、曲げ強さが155N/mm2と高い強度性能を示した。一般的な木材と比較すると、密度との関係において曲げ強さは同等であり、ヤング係数は高い傾向を示したが、逆に衝撃曲げ吸収エネルギーはやや低い傾向を示した。ユーカリ材のオオウズラタケおよびカワラタケに対する質量減少率は3.1~7.6%であり、中程度の耐朽性を示した。

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  • Evaluation of bonding durability of glued laminated timbers with outdoor exposure test (4) Results obtained after five years outdoor exposure

    柳川 靖夫, 宮崎 祐子, 和田 博

    Bulletin of the Nara Forest Research Institute   ( 36 )   19 - 25   2007.3

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    2種類の木材保存剤(ACQ、AZP)で処理したラミナを、2種類の接着剤(レゾルシノール系樹脂接着剤、水性高分子イソシアネート系樹脂接着剤)で接着して作製した、5プライのスギ集成材の屋外ばくろ試験を実施している。ばくろ5年後のせん断試験の結果、ACQ処理-レゾルシノール系樹脂接着剤の組み合わせで、せん断強度が低下する傾向が認められた。他の組合わせおよび木材保存剤で処理しなかったスギ集成材では、その傾向が観察されなかったことから、ACQの影響によるものと推測された。木材保護塗料は、木材の表面劣化を抑止するには効果的であった。しかし、木材保護塗料を2年ごとに塗布した場合であっても、ACQ処理-レゾルシノール系樹脂接着剤の組み合わせでは、接着はく離が発生するなど、接着強度の低下が観察された。ばくろ3年後までは、いずれの集成材でもほぼ100%であった木部破断率は、ばくろ5年後において低下した。しかし、木部破断率とせん断強度との間に相関は認められなかった。深木部破断率は、ACQ処理-レゾルシノール系樹脂接着剤の組み合わせでより低い値を示し、この組み合わせでは接着層界面近辺での破壊発生が増加することが示唆された。

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  • 竹林の拡大防止法としての竹切株への薬剤注入試験

    宮崎 祐子, 西尾 起一

    奈良県森林技術センター研究報告   ( 36 )   67 - 69   2007.3

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    竹林の拡大防止のため、薬剤を用いた竹の効率的な枯殺方法を検討した。竹林の皆伐のみを行った試験区と、皆伐後に切株へ薬剤を注入した試験区を比較した結果、皆伐後に切株へ薬剤を注入した試験区では、翌春の新稈発生本数が少なかった。また、7月下旬に処理を行った後約2ヶ月経過後までに、皆伐のみを行った試験区において皆伐後に切株へ薬剤の注入を行った試験区に比べて多数のササ状に矮性化した再生稈が認められた。これらのことから、皆伐後に切株へ薬剤の注入を行った試験区では、皆伐のみを行った試験区と比較して当年および翌年の新稈の発生が抑制されていると考えられる。

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  • ラミナを木材保存剤で処理した集成材の接着耐久性評価(第1報)繰り返しはくり試験における接着性能の検証

    増田 勝則, 柳川 靖夫, 宮崎 祐子

    奈良県森林技術センター研究報告   ( 35 )   37 - 41   2006.3

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    ラミナを木材保存剤で注入処理した集成材の接着耐久性評価として5種類(ACQ、AAC、NCU、NZN、CUAZ)の木材保存剤で処理したラミナと2種類のレゾルシノール樹脂系接着剤を組み合わせて作製した集成材の5回繰り返し煮沸はくり試験および減圧加圧試験を実施した。その結果、大部分の木材保存剤で処理したラミナを使用した集成材のはく離率は、ほぼ良好な値を示した。しかし、木材保存剤の種類と接着剤の種類によってはく離率に差があった。また、無処理の集成材と比較すると、保存剤で処理したラミナを使用した集成材には接着性能の低下が認められた。2種類のレゾルシノール樹脂系接着剤の間で接着性能に差が見られ、一方の接着剤ではNZNおよびACQによって注入処理したラミナを使用した集成材のはくり率の平均値が高く、NZNは5%を超えた。また、AACで処理したラミナを使用した集成材のはくり率は、すべての条件において低かった。

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  • スギ接着重ね梁の接着性能

    柳川 靖夫, 中田 欣作, 宮崎 祐子

    奈良県森林技術センター研究報告   ( 34 )   81 - 85   2005.3

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    スギ正角乾燥材および水性高分子-イソシアネート系接着剤を使用して製造された、2種類の接着重ね梁の接着性能を、同一産地のスギ材と同じ接着剤とを使用して製造された集成材の接着性能とともに調べた。浸せきはく離試験、煮沸はく離試験および減圧加圧試験の結果、正角材を2本使用した接着重ね梁のはく離率が最も高かった。次に、正角材2本と一方の外層にラミナを接着した接着重ね梁のはく離率が高かった。それらと比較して、集成材のはく離率はいずれの試験でも低く、ほとんどの試験体が構造用集成材のJASで規定されている接着性能を満たしていた。2種類の接着重ね梁のせん断強度の間に、差は認められなかった。接着重ね梁のせん断強度と、集成材のせん断強度との間には差が認められた。

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  • 屋外ばくろ試験による集成材の接着耐候性評価(第2報)接着耐候性評価に要する期間の短縮のための促進劣化試験

    宮崎 祐子, 和田 博, 満名 香織

    奈良県森林技術センター研究報告   ( 34 )   97 - 102   2005.3

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    防腐処理を施したラミナを接着して製造した集成材の接着耐候性を、短期間で評価することを目的とした促進劣化試験(煮沸はくり試験、減圧加圧試験、ブロックせん断試験片の屋外ばくろ試験、ブロックせん断試験片の減圧加圧試験)を行った。その結果、銅・アルキルアンモニウム化合物系防腐薬剤(ACQ)処理集成材は、プロペタンホス・アゾール化合物系防腐薬剤(AZP)処理集成材および無処理集成材と比較して接着性能が劣っていた。今後、各種の促進劣化試験のうち、どの処理が最も接着耐候性の予測に適しているかについて、集成材の屋外ばくろ試験の結果と照らし合わせ、解析することが必要である。

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  • 屋外ばくろ試験による集成材の接着耐候性評価(第3報)屋外ばくろ試験開始から3年経過後の結果

    宮崎 祐子, 和田 博, 満名 香織

    奈良県森林技術センター研究報告   ( 34 )   103 - 109   2005.3

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    集成材の接着耐候性を評価するため、銅・アルキルアンモニウム化合物系、ならびにプロペタンホス・アゾール化合物系防腐薬剤を加圧注入したスギラミナおよび無処理のスギラミナを接着して集成材を作製し、さらに木材保護塗料を塗布して、屋外ばくろ試験を行った。試験開始から3年経過後、保護塗料を塗布した集成材では、集成材側面に出現するはくり率の増加が抑制されていた。また、JASに準じたブロックせん断試験の結果、薬剤処理集成材のせん断強さが薬剤無処理集成材のせん断強さと比較して低くなる傾向がみられた。また、屋外ばくろ試験に供する前の集成材と比較した場合、保護塗料を塗布しなかった集成材において、せん断強さが若干低下していた。

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  • スギ接着重ね梁の曲げ強度試験

    中田 欣作, 柳川 靖夫, 宮崎 祐子, 小野 広治, 久保 健

    奈良県森林技術センター研究報告   ( 34 )   75 - 80   2005.3

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    奈良県産スギ材の構造用製材品への用途拡大を目的として、上北山村産のスギ材から得られた正角材を使用した接着重ね梁の曲げ試験を行って、以下の結果を得た。接着重ね梁は、高周波・蒸気複合乾燥したスギ正角材を2層重ねに接着したもの、およびこれに厚さ30mmのスギ材ラミナ1枚を接着し3層としたものを作製した。接着重ね梁の曲げ強さは、針葉樹の構造用製材の日本農林規格におけるスギ材の甲種構造用製材1級の基準曲げ強度をすべての試験体で上回っていた。しかし、それらは機械等級区分製材の基準曲げ強度を試験体18体のうち2つの試験体で下回った。2層接着重ね梁と3層接着重ね梁との比較では、曲げヤング係数は同等であったが、曲げ強さは後者のほうが高くなった。荷重とたわみの関係では、荷重が比例限度を越えると破壊に至る場合が多く認められた。

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Presentations

  • 雄性両全性同株ケツユクサにおける個花の環境応答型性決

    宮崎祐子

    日本植物学会第86回大会 シンポジウム「植物の多彩な生殖戦略を支える制御機構〜もう一度花成を考えてみる〜」  2022.9 

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    Event date: 2022.9

    Presentation type:Symposium, workshop panel (public)  

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  • 混殖種ツユクサの先行自家受粉は他殖の機会を減少させうるか?

    増田佳奈, 勝原光希, 宮崎祐子, 丑丸敦史

    日本生態学会第69回全国大会  2022.3 

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    Event date: 2022.3

    Presentation type:Oral presentation (general)  

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  • 西日本のブナ天然林における11年間のブナ実生個体群動態

    畑俊輔, 赤路康朗, 山田和弘, 中山陽介, 石井弘明, 東若菜, 野口結子, 牧本卓史, 坂本圭児, 廣部宗, 松本哲也, 宮崎祐子

    日本生態学会第69回全国大会  2022.3 

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    Event date: 2022.3

    Presentation type:Poster presentation  

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  • Plastic sex determination of flowers in andromonoecious Commelina communis f. ciliata

    2022.3 

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  • Effects of reproductive interference from closely related species on genetic structure and selfing rate in native Commelina species.

    勝原光希, 宮崎祐子, 丑丸敦史

    日本生態学会大会講演要旨(Web)  2021 

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  • Reproductive system of Pinellia tripartita (Araceae)

    尾上元哉, 松本哲也, 廣部宗, 宮崎祐子

    日本生態学会大会講演要旨(Web)  2021 

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  • 岡山県北部に分布する広義マムシグサを対象とした隠蔽種の探索

    平松勅悦, 松本哲也, 柿嶋聡, 宮崎祐子, 末吉昌宏, 廣部宗

    日本植物分類学会大会研究発表要旨集  2021 

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  • Floral sex determination related with resource dynamics within inflorescences in Commelina communis f. ciliata.

    佐藤弘大, 宮崎祐子, 勝原光希, 邑上夏菜, 廣部宗, 兵藤不二夫, 丑丸敦

    日本生態学会大会講演要旨(Web)  2021 

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  • Inter population floral variation caused by adaptation to local pollination environment.

    増田佳奈, 邑上夏菜, 勝原光希, 宮崎祐子, 丑丸敦史

    日本生態学会大会講演要旨(Web)  2021 

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  • Does urbanization influence popultion selfing rate in annual herb Commelina communis?

    増田佳奈, 邑上夏菜, 勝原光希, 宮崎祐子, 丑丸敦史

    日本生態学会大会講演要旨(Web)  2020 

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  • Pre-pollination isolating barriers between two sympatric species, Arisaema minus and A. yamatense

    松本哲也, 小林禧樹, 末吉昌宏, 宮崎祐子, 廣部宗

    日本生態学会大会講演要旨(Web)  2020 

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  • ブナにおける花芽分裂組織発達とFLOWERING LOCUS T相同遺伝子発現との関係

    宮崎祐子, 佐竹暁子, 北村系子

    日本森林学会大会学術講演集  2020 

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  • Relationship between the plasticity of floral sex determination and resources within inflorescences in Commelina communis f. ciliata

    佐藤弘大, 宮崎祐子, 勝原光希, 邑上夏菜, 廣部宗, 兵藤不二夫, 丑丸敦

    日本生態学会大会講演要旨(Web)  2020 

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  • テンナンショウ属5種の共存に対するキノコバエ類の種特異的訪花の寄与

    松本哲也, 廣部宗, 末吉昌宏, 宮崎祐子

    日本植物分類学会大会研究発表要旨集  2020 

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  • Sex determination of flowers depending on day length and nutrient environment in andromonoecious species Commelina communis f. ciliata

    宮崎祐子, 佐藤弘大, 井上智美, 赤路康朗, 勝原光希, 邑上夏菜, 丑丸敦史

    日本生態学会大会講演要旨(Web)  2020 

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  • 局所的環境条件がブナ若齢実生の生残と成長に及ぼす影響

    山田和弘, 赤路康朗, 宮崎祐子, 安達亮太, 谷口武士, 兵藤不二夫, 坂本圭児, 廣部宗

    日本生態学会大会講演要旨(Web)  2019.2.9 

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    Event date: 2019.2.9

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  • 落葉広葉樹二次林における優占種コナラの成長に影響を与える環境要因

    伊藤蒼, 兵藤不二夫, 松本哲也, 赤路康朗, 宮崎祐子, 廣部宗, 西村尚之, 坂本圭児

    日本生態学会大会講演要旨(Web)  2019.2.9 

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    Event date: 2019.2.9

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  • 雄性両全性同株ケツユクサの花の性は資源依存的に決まるのか?

    佐藤弘大, 宮崎祐子, 勝原光希, 邑上夏菜, 廣部宗, 兵藤不二夫, 丑丸敦史

    日本生態学会大会講演要旨(Web)  2019.2.9 

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  • 送粉環境と花形質が自殖率に与える影響:ツユクサの集団間変異を用いた検証

    邑上夏菜, 勝原光希, 宮崎祐子, 丑丸敦史

    日本生態学会大会講演要旨(Web)  2019 

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  • ブナの花芽形成決定への個体内資源量の関与

    宮崎祐子, JOSEPH Benesh, 小林正樹, 清水健太郎, 佐竹暁子

    日本生態学会大会講演要旨集  2010.3.15 

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  • Classical Phenotype-Based Taxonomy Was Ratified by Genome-Based One (II) : Classification of wide range ('Order' difference) insects and identical species of a plant

    KOMAZAKI Shun, AHMED Shamin, TAKAHASHI Masahiro, UENO Sachika, KOMORI Manabu, BIYANI Manish, MIYAZAKI Yuko, SATAKE Akiko, KOSAKU Akinori, MIYAMOTO Kiyoshi, NISHIGAKI Koichi

    形の科学会誌 = Bulletin of the Society for Science on Form  2009.11.1 

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    Event date: 2009.11.1

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  • ササは一回繁殖か?‐クマイザサ個体群の開花様式‐

    宮崎祐子, 大西尚樹, 日野貴文, 日浦勉

    日本生態学会大会講演要旨集  2006.3.24 

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  • 光合成同化産物の果実への転流パターン 13Cトレース法による追跡

    宮崎祐子, 船田良, 日浦勉

    日本生態学会大会講演要旨集  2002.3.25 

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    Language:Japanese  

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  • ハクウンボクの繁殖に対する資源分配

    宮崎祐子, 船田良, 日浦勉

    日本生態学会大会講演要旨集  2001.3.22 

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    Event date: 2001.3.22

    Language:Japanese  

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  • キノコバエ送粉者の花への選好性は日本産テンナンショウ属の種同定に応用できるか?

    松本哲也, 柿嶋聡, 小林禧樹, 前川奈々子, 多々納琴音, 大野順一, 廣部宗, 末吉昌宏, 狩山俊悟, 宮崎祐子

    第55回種生物学シンポジウム  2023.12 

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  • 岡山市の都市域-中山間地域における在来一年草ツユクサの集団遺伝学的解析と送粉者調査

    山本倫正, 山口寛登, 藤原日向, 宮崎祐子, 中田泰地, 中濱直之, 中田和義, 勝原光希

    第55回種生物学シンポジウム  2023.12 

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  • 発芽後の環境がブナ当年生実生の定着に与える影響

    多々納琴音, 松本哲也, Vu Khanh Linh, 廣部宗, 坂本圭児, 宮崎祐子

    日本生態学会中国四国地区会第66回大会  2023.5 

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  • 成⻑特性の異なるスギ品種間での樹幹部における発現変動遺伝⼦解析

    宮崎祐子, 東若菜, Uchiyama Christmas, 高木正博, 石井弘明

    第134回日本森林学会大会  2023.3 

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  • Identification of genes regulating the bud growth of Quercus serrata in winter and summer

    2023.3 

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  • 岡山県産広義マムシグサを対象とした訪花キノコバエ相に基づく隠蔽種の探索

    松本哲也, 平松勅悦, 柿嶋 聡, 宮崎祐子, 末吉昌宏, 廣部 宗

    日本植物分類学会 第22回大会  2023.3 

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  • 混生するテンナンショウ属2種の繁殖様式と開花・性転換閾値の比較

    松本哲也, 小林禧樹, 末吉昌宏, 宮﨑祐子, 廣部 宗

    第53回種生物学シンポジウム  2021.12 

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  • 岡山県北部に分布する広義マムシグサにおける訪花昆虫相と形態に基づく隠蔽種の探索

    平松勅悦, 松本哲也, 末吉昌宏, 宮﨑祐子, 廣部 宗

    第52回種生物学シンポジウム  2020.12 

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  • テンナンショウ属5種に訪花するキノコバエ類の種特異性と生殖隔離としての潜在的機能

    松本哲也, 廣部 宗, 末吉昌宏, 宮﨑祐子

    第52回種生物学シンポジウム  2020.12 

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  • Influence of intermittent seed production on canopy growth and seedling recruitment in Fagus crenata

    Yuko Miyazaki

    2019.3 

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  • The effect on growth of young beech seedlings by light environment and soil environment

    Yamada Kazuhiro, Akaji Yasuaki, Hirobe Muneto, Hyoudou Fujio, Miyazaki Yuhko, Sakamoto Keiji

    The Japanese Forest Society Congress  2018 

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    <p>[in Japanese]</p>

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  • Dynamics of dominant tree species for 24 years in an old-growth beech forest, western Japan.

    Makimoto Takushi, Akaji Yasuaki, Kinoshita Shuu, Miyazaki Yuko, Hirobe Muneto, Mizunaga Hiromi, Sakamoto Keiji

    The Japanese Forest Society Congress  2018 

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    <p>[in Japanese]</p>

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  • Spatial and temporal variation in emergence and survival of Fagus crenata seedlings

    Akaji Yasuaki, Hirobe Muneto, Miyazaki Yuko, Makimoto Takushi, Sakamoto Keiji

    The Japanese Forest Society Congress  2016 

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    [in Japanese]

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  • Regeneration dynamics of Fagus crenata in Mt. Hyonosen

    Akaji Yasuaki, Kinoshita Shu, Uyanga Ariya, Miyazaki Yuko, Hirobe Muneto, Makimoto Takushi, Mizunaga Hiromi, Sakamoto Keiji

    The Japanese Forest Society Congress  2015 

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    [in Japanese]

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  • Influence of nitrogen fertilization on reproduction of tree species

    Miyazaki Yuko, Kanehara Yuuki, Lee Jin, Osada Noriyuki, Nakaji Tatsuro, Hiura Tsutom

    The Japanese Forest Society Congress  2015 

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  • Effects of canopy tree species on survival and growth of Fagus crenata understory trees in an old beech forest

    Kinoshita Shu, Akaji Yasuaki, Makimoto Takushi, Miyazaki Yuko, Hirobe Muneto, Mizunaga Hiroki, Sakamoto Keiji, Yamamoto Shin-ichi

    The Japanese Forest Society Congress  2014 

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    [in Japanese]

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  • Relationships between the growth and survival of Fagus crenata seedlings and environmental conditions

    Akaji Yasuaki, Makimoto Takushi, Kinoshita Shu, Miyazaki Yuko, Hirobe Muneto, Sakamoto Keiji

    The Japanese Forest Society Congress  2014 

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  • Stand dynamics and dendroecology of an old-growth beech forest in southeastern Japan

    Ariya Uyanga, Yamanoto Shin-Inchi, Hamano Ken-Ya, Makimoto Takushi, Akazi Yasuaki, Kinoshita Syuu, Miyazaki Yuko, Hirobe Muneto, Mizunaga Hiromi, Sakamoto Keiji

    The Japanese Forest Society Congress  2013 

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    Dendro-ecological analysis is needed to understand stand dynamics of forests by estimating the disturbance history and age structure. The analysis was conducted in the old-growth beech forest of Wakasugi forest reserve (N35&rsquo;14&rsquo;, E135&rsquo;23&rsquo;), southwestern Japan. Increment cores were taken from all the tree individuals with dbh of more than 10cm, for tree ring analysis in the plots of 50m x 130m. For the disturbance history analysis, boundary-line release criteria were adopted to identify significant growth releases in each tree ring series, which are likely to be caused by disturbances. As results, age structure suggested continuous establishment by Fagus crenata and simultaneous establishment by Mognolia obovata. Disturbance history analysis showed that in this beech forest several disturbance events happened for 180 years. We are comparing the result with historical materials and discussing relationships between disturbance history and establishment of tree.

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  • Analysis of flowering gene (FcLFY) expression in Fagus crenata

    Miyazaki Yuko, Kobayashi J. Masaki, Hiura Tsutom, Kon Hirokazu, Shimizu K. Kentaro, Satake Akiko

    Plant and Cell Physiology Supplement  2011 

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    Fagus crenata produce intermittently large numbers of seeds or flowers, not annual reproduction. Several hypotheses have been developed to explain the mechanism of intermittent reproduction; i.e. internal resources, climate cues. These proximate factors assume that the plant sets flowers as a result of that these factors induce differentiation of floral meristem as a starting signal. However, there are no direct evidences about whether these factors function as a signal of floral initiation, because it cannot be detected when the first switch of flowering on. In order to explore these factors on floral initiation, we quantified expression level of flower identity gene; LEAFY homolog (FcLFY) as well as process of flower bud differentiation using mature F. crenata. We measured seasonal pattern of FcLFY expression in bud using real-time quantitative PCR. In addition, we observed the relationship between FcLFY expression in bud and process of flower bud differentiation. We also analyzed relationship between FcLFY expression and internal resources. This approach could allow us to evaluate which proximate factor works as a signal of floral initiation in F. crenata.

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  • Dynamics of the forest communities in old-growth beech forest during 19-year study:western Japan.

    Kinoshita Shu, Akaji Yasuaki, Makimoto Takushi, Miyazaki Yuko, Hirobe Muneto, Mizunaga Hiroki, Sakamoto Keiji, Yamamoto Shinnichi

    The Japanese Forest Society Congress  2011 

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  • Phylogeographical analysis by genotyping chloroplast DNA in Zelkova serrata

    Fukatsu Eitaro, Shimada Hiromasa, Takii Tadato, Kabemura Yuji, Ubukata Masatoshi, Takahashi Makoto, Nakada Ryogo, Yano Keisuke, Watanabe Atsushi, Koyama Yasuhiro, Odani Jiro, Saito Maki, Miyazaki Yuko

    The Japanese Forest Society Congress  2008 

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Awards

  • ブナセンター賞

    2012.2   黒松内生物多様性保全奨励事業運営委員会  

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Research Projects

  • 樹木の結実豊凶に対する散布前種子捕食者の役割の解明

    Grant number:24K01816  2024.04 - 2029.03

    日本学術振興会  科学研究費補助金  基盤研究(B)

    平山貴美子, 宮崎祐子

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  • コナラ属における開芽・展葉制御とその種間・集団間変異の遺伝基盤の解明

    Grant number:22K05756  2022.04 - 2026.03

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    小林 正樹, 宮崎 祐子

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    Grant amount:\4160000 ( Direct expense: \3200000 、 Indirect expense:\960000 )

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  • Evaluating physiological plasticity of trees to climate change using functional trait variation of xylem anatomy

    Grant number:20H03031  2020.04 - 2024.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    石井 弘明, 榎木 勉, 鍋嶋 絵里, 宮崎 祐子, 東 若菜, 鵜川 信

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    Grant amount:\12480000 ( Direct expense: \9600000 、 Indirect expense:\2880000 )

    (1)宮崎大学演習林のスギクローン試験地において、スギ品種ごとの肥大成長速度、木部組織および水分通道の違いについて、木部資料の採取および測定機器の設置によって調査した。
    (2)スギの物質生産に遺伝および環境要因が及ぼす影響を明らかにするために,複数の地域に設置された共通圃場において,品種による生産量,窒素利用,成長様式などの違いを定量
    化する。
    (3)成長特性の異なるスギ2系統の形成層帯で発現する遺伝子群の季節変化を把握するため、2系統4個体ずつから採取した組織についてRNA-seqを行った。
    (4)愛媛大学農学部附属演習林において、スギ品種ごとの年輪試料採取を行い、安定同位体比測定のための試料調整を行った。
    (5)鹿児島大学高隈演習林にて、スギ6品種の細根形質を調べたところ、細根形質の品種間差を確認したが、斜面位置にともなう細根形質の順応的変化は検出されなかった。

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  • Adaptive significance and mechanism of environment-dependent sex determination in flowers

    Grant number:19K06855  2019.04 - 2022.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    宮崎 祐子, 丑丸 敦史, 井上 智美

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    Grant amount:\4290000 ( Direct expense: \3300000 、 Indirect expense:\990000 )

    本年度は雄性両全性同株ケツユクサの栄養状態が両性花を誘導するメカニズムについて,ケツユクサの1番目の両性花の結実成功に伴う子房への資源配分による花序内の資源制限が2番目の花を雄花へ誘導しているという仮説を立て,1番目の花の開花から24時間以内の花序内の資源動態を調べることで,資源制限と雄花誘導の関係性を検証した.
    開花期に無作為に選んだ花序の1番目の両性花に対し,開花直後に結実成功/失敗のどちらかの処理を施し,2番目の花の性を操作し,以下のように花序の各部位を採取した.2番目の花の性が決まる24時間以内の資源配分を調べるために1番目の花の子房を,さらに,その資源配分に伴う花序内の資源濃度の変化を調べるために総苞,花柄,2番目の花のつぼみを採取した.また,両性花および雄花生産に要するコストを調べるために,性を操作した2番目の花のつぼみを処理直後から開花直前の60時間後まで追跡し採取を行った.その後,各器官において繁殖器官に多く含まれ,制限要因となりやすい窒素(N)とリン(P)の濃度を測定し,各処理および採取時間において比較した.
    1番目の花の開花から24時間以内に結実成功に伴う子房への資源配分は確認された一方で,子房への資源配分に同調する花序の各部位での資源濃度の減少はみられなかった.花柄においては結実成功に伴うN濃度の増加がみられ,花序外からのNの流入が示唆された.また,開花直前までのつぼみの資源濃度には花の性による違いがなかったものの,質量として算出したNおよびP含有量で比較したところ,先行研究同様,花生産に要するコストは雄花の方が安価であることが示された.よって,花序内の資源制限が安価な雄花の生産を誘導するという仮説は支持されず,ケツユクサ花序の2番目の花の雄花誘導はNおよびP濃度の制限によるものではないことが考えられた.

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  • ブナ天然林を支える外生菌根菌の多様性と菌糸ネットワークによる実生更新への貢献の解明

    2018.07 - 2019.03

    公益財団法人 八雲環境科学振興財団  環境研究助成(特定研究) 

    宮崎 祐子

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    Authorship:Principal investigator  Grant type:Competitive

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  • 暖温帯林におけるブナ科樹種の構成割合の変化が種子生産の年変動に与える影響の解明

    Grant number:18K05750  2018.04 - 2022.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    平山 貴美子, 宮崎 祐子

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    Grant amount:\4550000 ( Direct expense: \3500000 、 Indirect expense:\1050000 )

    温帯域の森林の主要構成種であるブナ科樹種の種子(堅果)は、開花直後から結実に至るまで多くの昆虫に加害され、それが種子生産に大きな影響を与えることが知られている。西日本の暖温帯域では、遷移の進行などにより林分におけるブナ科樹種の構成割合が変化しており、それが種子を加害する昆虫の密度や加害パターンを変化させ、各ブナ科樹種の種子生産の年変動に影響している可能性がある。本研究では、このような散布前の種子への加害といった生物的要因の着目し、それが種子生産量の変動に及ぼす影響を定量的に明らかにすることを目的としている。2019年度の研究成果は以下の通りである。
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    1.西日本の二次林の代表種であるコナラについて、アベマキ・アラカシといった多種のコナラ属が混交している林と、コナラが優占している林において、コナラの散布前種子の加害に大きく関わっていることが明らかになってきたハイイロチョッキリの個体密度を推定したところ、多種のコナラ属が混交している林においてその個体密度が高く、コナラの種子生産が大きく制限されている可能性が示唆された。
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    2.京都盆地周辺で分布が拡大しているコジイについて、最近コジイが優占してきた林(宝ヶ池)、京都でも古くからコジイが優占している林(東山)、自然状態の照葉樹林が残存する林(宮崎県綾)において種子生産や種子食昆虫の加害の影響を調べたところ、「東山」と「綾」では、種子食昆虫の影響が大きく、それにより種子生産が大きく年変動していることが明らかとなった。一方で、最近コジイが優占してきた「宝ヶ池」では種子食昆虫の影響はとても小さく、毎年多くの種子が生産されていた。林分の古さが種子加害者の密度に影響し、それが種子生産に影響している可能性が示唆された。

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  • Study on the mechanism of sexual expression of individual flowers in plants due to resource limitation

    Grant number:16K07517  2016.04 - 2020.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    MIYAZAKI Yuko

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    Grant amount:\4550000 ( Direct expense: \3500000 、 Indirect expense:\1050000 )

    It has been suggested that internal resources are involved in the mechanism of plastic sexual expression determination of individual flowers in plants, but the details are unknown. In this study, we tested whether locally occurring resource limitations within individuals could explain the sex determination of individual flowers.
    Using Commelina communis f. ciliata, which has hermaphroditic and male flowers in a single plant, we examined the existence of fruiting of first flower (B1) in the inflorescence, and sex expression and resources of next blooming flower (B2). We also performed a comparative transcriptome analysis of male and female flowers. As a result, the decrease of resources in the inflorescence was not remarkable, and the nitrogen and phosphorus concentration in the bud of B2 did not differ by the existence of B1 fruiting. These results suggest that the resource limitation in the inflorescence is not a factor that directly controls the sex of B2.

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  • シカの食害による林床環境の改変がテンナンショウ属の性転換と個体群動態に与える影響

    2015

    公益財団法人 八雲環境科学振興財団  環境研究助成 

    宮崎 祐子

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    Authorship:Principal investigator  Grant type:Competitive

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  • テンナンショウ属を用いた植物の性転換機構の解明

    2015

    公益財団法人 ウエスコ学術振興財団  学術研究費助成 

    宮崎 祐子

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    Authorship:Principal investigator  Grant type:Competitive

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  • テンナンショウ属を用いた植物の性表現決定機構の解明

    2015

    公益財団法人 両備檉園記念財団  研究助成金 

    宮崎 祐子

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  • Initial responses of nutrient cycling and biodiversity to a large-scale N fertilization in a carbon flux monitoring site.

    Grant number:25292079  2013.04 - 2016.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    HIURA Tsutom, TOKUCHI Naoko, MIYAZAKI Yuko, NAKAJI Tatsuro, KATAYAMA Ayumi, MAKITA Naoki, NAKAMURA Masahiro, Lee Jin

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    Grant amount:\17030000 ( Direct expense: \13100000 、 Indirect expense:\3930000 )

    We revealed the initial response of below-ground carbon dynamics, N and P dynamics, reproduction of trees, and herbivory by insects to N deposition in a cool temperate forest ecosystem. We fertilized 10 ha in area, and established 9 ha control site during 3 years in a secondary deciduous broad-leaved forest of Tomakomai Experimental Forest, Hokkaido University.
    Biomass of fine roots, soil respiration rates, and phosphoric acids were increased by N fertilization. Reproductive organs of some tree species were tended to increase by the manipulation. Leaf traits of oak and alder species were altered but herbivory rates were not changed in the second year after treatment. In third year, diversity of herbivory insects decreased in the fertilized site.

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  • Elucidation of impact of environmental change on the flowering and fruiting in Quercus species

    Grant number:24770021  2012.04 - 2016.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    Miyazaki Yuko

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    Grant amount:\4680000 ( Direct expense: \3600000 、 Indirect expense:\1080000 )

    Year-to-year variation in the amount of flower and fruit can be seen in many plant species. In this study, I conducted following analysis. 1) investigating the changes of the amount of the flower and fruit by the field experiment manipulating the environmental conditions, 2) figuring out the gene expression changes that occur from flower bud formation to seed maturation under the same experimental environment, 3) extracting the proximate factor causing the annual variation, using two Quercus species. The high temperature had a possibility to affect positively for current fruiting, while it had negative effect for flowering in the following year. In addition, fertilization experiment suggested that nitrogen may alleviate the requirement for seed production, even if a factor to determine seed amount except nitrogen availability (e.g., temperature or precipitation) was inappropriate.

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  • Effect of long-term application of fertilizer on masting of Betula

    Grant number:23570041  2011.04 - 2016.03

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    Ito Eriko, HASEGAWA Shigeaki, MIYAZAKI Yuko

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    Grant amount:\5330000 ( Direct expense: \4100000 、 Indirect expense:\1230000 )

    To clarify storage resources linking masting based on the resource budget hypothesis, we investigated a sequential variation of quantity of resources (non-structure-related carbohydrate (NSC) and nitrogen (N) ) stored in the Betula maximowicziana trees during five years including two masting years. Masting cycle of B. maximowicziana was related with NSC storage. NSC accumulated in the whole tree body during non-masting years, whereas N storage was relatively constant. The NSC density of the trunk, i.e., a large-capacity and low-priority storage organ, was clearly indicated the masting cycle.

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  • ブナセンター賞助成金

    2011

    平成23年度黒松内生物多様性保全奨励事業 

    宮崎 祐子

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    Authorship:Principal investigator  Grant type:Competitive

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  • Biology (Environment, Ecology and Evolution) (2023academic year) Fourth semester  - 木1~2

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Media Coverage

  • なぜ? 全道でササが一斉開花、枯死 数十年から百数十年に1度 シカ餌不足の恐れも Newspaper, magazine

    北海道新聞  2023.8.14

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